Science China Life Sciences最新文献

Research landmarks on the 60th anniversary of Epstein-Barr virus. Epstein-Barr 病毒 60 周年研究里程碑。

IF 8 2区生物学

Science China Life Sciences Pub Date : 2024-11-04 DOI: 10.1007/s11427-024-2766-0

Lan-Yi Zhong, Chu Xie, Le-Le Zhang, Yan-Lin Yang, Yuan-Tao Liu, Ge-Xin Zhao, Guo-Long Bu, Xian-Shu Tian, Zi-Ying Jiang, Bo-Yu Yuan, Peng-Lin Li, Pei-Huang Wu, Wei-Hua Jia, Christian Münz, Benjamin E Gewurz, Qian Zhong, Cong Sun, Mu-Sheng Zeng

引用次数: 0

COVID-19 vaccine updates for people under different conditions. 针对不同人群的 COVID-19 疫苗更新。

IF 8 2区生物学

Science China Life Sciences Pub Date : 2024-11-01 Epub Date: 2024-07-29 DOI: 10.1007/s11427-024-2643-1

Yijiao Huang, Weiyang Wang, Yan Liu, Zai Wang, Bin Cao

{"title":"COVID-19 vaccine updates for people under different conditions.","authors":"Yijiao Huang, Weiyang Wang, Yan Liu, Zai Wang, Bin Cao","doi":"10.1007/s11427-024-2643-1","DOIUrl":"10.1007/s11427-024-2643-1","url":null,"abstract":"SARS-CoV-2 has caused global waves of infection since December 2019 and continues to persist today. The emergence of SARS-CoV-2 variants with strong immune evasion capabilities has compromised the effectiveness of existing vaccines against breakthrough infections. Therefore, it is important to determine the best utilization strategies for different demographic groups given the variety of vaccine options available. In this review, we will discuss the protective efficacy of vaccines during different stages of the epidemic and emphasize the importance of timely updates to target prevalent variants, which can significantly improve immune protection. While it is recognized that vaccine effectiveness may be lower in certain populations such as the elderly, individuals with chronic comorbidities (e.g., diabetes with poor blood glucose control, those on maintenance dialysis), or those who are immunocompromised compared to the general population, administering multiple doses can result in a strong protective immune response that outweighs potential risks. However, caution should be exercised when considering vaccines that might trigger an intense immune response in populations prone to inflammatory flare or other complications. In conclusion, individuals with special conditions require enhanced and more effective immunization strategies to prevent infection or reinfection, as well as to avoid the potential development of long COVID.","PeriodicalId":21576,"journal":{"name":"Science China Life Sciences","volume":null,"pages":null},"PeriodicalIF":8.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141856426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Versatile and efficient mammalian genome editing with Type I-C CRISPR System of Desulfovibrio vulgaris. 利用普通脱硫弧菌的 I-C 型 CRISPR 系统进行多功能、高效的哺乳动物基因组编辑。

IF 8 2区生物学

Science China Life Sciences Pub Date : 2024-11-01 Epub Date: 2024-08-07 DOI: 10.1007/s11427-023-2682-5

Pan Li, Dingcai Dong, Fei Gao, Yuyang Xie, Honglin Huang, Siwei Sun, Zhao Ma, Cheng He, Jinsheng Lai, Xuguang Du, Sen Wu

{"title":"Versatile and efficient mammalian genome editing with Type I-C CRISPR System of Desulfovibrio vulgaris.","authors":"Pan Li, Dingcai Dong, Fei Gao, Yuyang Xie, Honglin Huang, Siwei Sun, Zhao Ma, Cheng He, Jinsheng Lai, Xuguang Du, Sen Wu","doi":"10.1007/s11427-023-2682-5","DOIUrl":"10.1007/s11427-023-2682-5","url":null,"abstract":"CRISPR-Cas tools for mammalian genome editing typically rely on single Cas9 or Cas12a proteins. While type I CRISPR systems in Class I may offer greater specificity and versatility, they are not well-developed for genome editing. Here, we present an alternative type I-C CRISPR system from Desulfovibrio vulgaris (Dvu) for efficient and precise genome editing in mammalian cells and animals. We optimized the Dvu type I-C editing complex to generate precise deletions at multiple loci in various cell lines and pig primary fibroblast cells using a paired PAM-in crRNA strategy. These edited pig cells can serve as donors for generating transgenic cloned piglets. The Dvu type I-C editor also enabled precise large fragment replacements with homology-directed repair. Additionally, we adapted the Dvu-Cascade effector for cytosine and adenine base editing, developing Dvu-CBE and Dvu-ABE systems. These systems efficiently induced C-to-T and A-to-G substitutions in human genes without double-strand breaks. Off-target analysis confirmed the high specificity of the Dvu type I-C editor. Our findings demonstrate the Dvu type I-C editor's potential for diverse mammalian genome editing applications, including deletions, fragment replacement, and base editing, with high efficiency and specificity for biomedicine and agriculture.","PeriodicalId":21576,"journal":{"name":"Science China Life Sciences","volume":null,"pages":null},"PeriodicalIF":8.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141913674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A CRISPR/RfxCas13d-mediated strategy for efficient RNA knockdown in mouse embryonic development. CRISPR/RfxCas13d 介导的小鼠胚胎发育中高效 RNA 敲除策略。

IF 8 2区生物学

Science China Life Sciences Pub Date : 2024-11-01 Epub Date: 2024-08-02 DOI: 10.1007/s11427-023-2572-6

Lin Zhang, Shi-Meng Cao, Hao Wu, Meng Yan, Jinsong Li, Ling-Ling Chen

引用次数: 0

Activation of the PGC-1α-mediated mitochondrial glutamine metabolism pathway attenuates female offspring osteoarthritis induced by prenatal excessive prednisone. 激活 PGC-1α 介导的线粒体谷氨酰胺代谢途径可减轻产前过量泼尼松诱导的雌性后代骨关节炎。

IF 8 2区生物学

Science China Life Sciences Pub Date : 2024-11-01 Epub Date: 2024-08-21 DOI: 10.1007/s11427-023-2593-4

Qingxian Li, Fan Zhang, Yongguo Dai, Liang Liu, Liaobin Chen, Hui Wang

{"title":"Activation of the PGC-1α-mediated mitochondrial glutamine metabolism pathway attenuates female offspring osteoarthritis induced by prenatal excessive prednisone.","authors":"Qingxian Li, Fan Zhang, Yongguo Dai, Liang Liu, Liaobin Chen, Hui Wang","doi":"10.1007/s11427-023-2593-4","DOIUrl":"10.1007/s11427-023-2593-4","url":null,"abstract":"Osteoarthritis is a chronic, age-related joint disease. Previous studies have shown that osteoarthritis develops during intrauterine development. Prednisone is frequently used to treat pregnancies complicated by autoimmune diseases. However, limited research has been conducted on the enduring effects of prednisone use during pregnancy on the offspring. In this study, we investigated the effect of excessive prednisone exposure on cartilage development and susceptibility to osteoarthritis in the offspring. We found that prenatal prednisone exposure (PPE) impaired cartilage extracellular matrix (ECM) synthesis, resulting in poor cartilage pathology in female offspring during the adult period, which was further exacerbated after long-distance running stimulation. Additionally, PPE suppressed cartilage development during the intrauterine period. Tracing back to the intrauterine period, we found that Pred, rather than prednisone, decreased glutamine metabolic flux, which resulted in increased oxidative stress, and decreased histone acetylation, and expression of cartilage phenotypic genes. Further, PGC-1α-mediated mitochondrial biogenesis, while PPE caused hypermethylation in the promoter region of PGC-1α and decreased its expression in fetal cartilage by activating the glucocorticoid receptor, resulting in a reduction of glutamine flux controlled by mitochondrial biogenesis. Additionally, overexpression of PGC-1α (either pharmacological or through lentiviral transfection) reversed PPE- and Pred-induced cartilage ECM synthesis impairment. In summary, this study demonstrated that PPE causes chondrodysplasia in female offspring and increases their susceptibility to postnatal osteoarthritis. Hence, targeting PGC-1α early on could be a potential intervention strategy for PPE-induced osteoarthritis susceptibility.","PeriodicalId":21576,"journal":{"name":"Science China Life Sciences","volume":null,"pages":null},"PeriodicalIF":8.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142047147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innovative genome editing in plants: a transposase and CRISPR combination approach. 创新的植物基因组编辑：转座酶和 CRISPR 组合方法。

IF 8 2区生物学

Science China Life Sciences Pub Date : 2024-11-01 DOI: 10.1007/s11427-024-2729-2

Hamza Sohail, Iqra Noor, Xuehao Chen, Xiaodong Yang

引用次数: 0

Androgens exert multifaceted functions in sex differences analyzed through single-cell transcriptome. 通过单细胞转录组分析雄激素在性别差异中发挥的多方面功能

IF 8 2区生物学

Science China Life Sciences Pub Date : 2024-11-01 Epub Date: 2024-06-26 DOI: 10.1007/s11427-024-2652-y

Xinxin Tang, Yinkun Fu, Zhihui Zou, Yue Li, Ming He

引用次数: 0

Zika virus infection induces glycometabolic disorder in northern pig-tailed macaques. 寨卡病毒感染会诱发北方猪尾猕猴糖代谢紊乱。

IF 8 2区生物学

Science China Life Sciences Pub Date : 2024-11-01 Epub Date: 2024-08-30 DOI: 10.1007/s11427-024-2663-6

Qing Li, Ren-Hua Yang, Yan Hu, Bei-Bei Tang, Ying-Jie Jiang, Chang-Bo Zheng, Tian-Zhang Song

引用次数: 0

Temperature regulates negative supercoils to modulate meiotic crossovers and chromosome organization. 温度调节负超螺旋，从而调节减数分裂交叉和染色体组织。

IF 8 2区生物学

Science China Life Sciences Pub Date : 2024-11-01 Epub Date: 2024-07-23 DOI: 10.1007/s11427-024-2671-1

Yingjin Tan, Taicong Tan, Shuxian Zhang, Bo Li, Beiyi Chen, Xu Zhou, Ying Wang, Xiao Yang, Binyuan Zhai, Qilai Huang, Liangran Zhang, Shunxin Wang

{"title":"Temperature regulates negative supercoils to modulate meiotic crossovers and chromosome organization.","authors":"Yingjin Tan, Taicong Tan, Shuxian Zhang, Bo Li, Beiyi Chen, Xu Zhou, Ying Wang, Xiao Yang, Binyuan Zhai, Qilai Huang, Liangran Zhang, Shunxin Wang","doi":"10.1007/s11427-024-2671-1","DOIUrl":"10.1007/s11427-024-2671-1","url":null,"abstract":"Crossover recombination is a hallmark of meiosis that holds the paternal and maternal chromosomes (homologs) together for their faithful segregation, while promoting genetic diversity of the progeny. The pattern of crossover is mainly controlled by the architecture of the meiotic chromosomes. Environmental factors, especially temperature, also play an important role in modulating crossovers. However, it is unclear how temperature affects crossovers. Here, we examined the distribution of budding yeast axis components (Red1, Hop1, and Rec8) and the crossover-associated Zip3 foci in detail at different temperatures, and found that both increased and decreased temperatures result in shorter meiotic chromosome axes and more crossovers. Further investigations showed that temperature changes coordinately enhanced the hyperabundant accumulation of Hop1 and Red1 on chromosomes and the number of Zip3 foci. Most importantly, temperature-induced changes in the distribution of axis proteins and Zip3 foci depend on changes in DNA negative supercoils. These results suggest that yeast meiosis senses temperature changes by increasing the level of negative supercoils to increase crossovers and modulate chromosome organization. These findings provide a new perspective on understanding the effect and mechanism of temperature on meiotic recombination and chromosome organization, with important implications for evolution and breeding.","PeriodicalId":21576,"journal":{"name":"Science China Life Sciences","volume":null,"pages":null},"PeriodicalIF":8.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141760792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling potential sex-determining genes and sex-specific markers in autotetraploid Carassius auratus. 揭示自交系鲫鱼的潜在性别决定基因和性别特异性标记。

IF 8 2区生物学

Science China Life Sciences Pub Date : 2024-11-01 Epub Date: 2024-08-09 DOI: 10.1007/s11427-023-2694-5

Kun Zhang, Xu Huang, Chongqing Wang, Xidan Xu, Xiaowei Xu, Xiaoping Dong, Qingwen Xiao, Jinhai Bai, Yue Zhou, Zhengkun Liu, Xinyi Deng, Yan Tang, Siyang Li, Enkui Hu, Wanjing Peng, Ling Xiong, Qinbo Qin, Shaojun Liu

{"title":"Unveiling potential sex-determining genes and sex-specific markers in autotetraploid Carassius auratus.","authors":"Kun Zhang, Xu Huang, Chongqing Wang, Xidan Xu, Xiaowei Xu, Xiaoping Dong, Qingwen Xiao, Jinhai Bai, Yue Zhou, Zhengkun Liu, Xinyi Deng, Yan Tang, Siyang Li, Enkui Hu, Wanjing Peng, Ling Xiong, Qinbo Qin, Shaojun Liu","doi":"10.1007/s11427-023-2694-5","DOIUrl":"10.1007/s11427-023-2694-5","url":null,"abstract":"Autotetraploid Carassius auratus is a stable hereditary autotetraploid fish resulting from the hybridization of Carassius auratus red var. (RCC, ♀) × Megalobrama amblycephala (BSB, ♂), containing four sets of RCC chromosomes. However, the molecular mechanism underlying the determination of sex in this species remains largely unknown. Currently, there lacks a full understanding of the molecular mechanisms governing sex determination and specific molecular markers to differentiate sex in this species. In this study, 25,801,677 SNPs (Single-nucleotide polymorphism) and 6,210,306 Indels (insertion-deletion) were obtained from whole-genome resequencing of 100 individuals (including 50 female and 50 male). Further identification confirmed the candidate chromosomes as Chr46B, with the sex-determining region located at Chr46B: 22,500,000-22,800,000 bp. Based on the male-specific insertion (26 bp) within the candidate sex-determining region, a pair of sex-specific molecular markers has been identified. In addition, based on the screening of candidate sex-determining region genes and RT-qPCR validation analysis, ADAM10, AQP9 and tc1a were identified as candidate sex-determining genes. These findings provide a robust foundation for investigating sex determination mechanisms in fish, the evolution of sex chromosomes, and the development of monosex populations.","PeriodicalId":21576,"journal":{"name":"Science China Life Sciences","volume":null,"pages":null},"PeriodicalIF":8.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141971799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0