New Phytologist最新文献

{"title":"Consolidating Ulva functional genomics: gene editing and new selection systems","authors":"Jonas Blomme, Júlia Arraiza Ribera, Olivier De Clerck, Thomas B. Jacobs","doi":"10.1111/nph.70068","DOIUrl":"https://doi.org/10.1111/nph.70068","url":null,"abstract":"<p>\u0000</p><ul>\u0000<li>The green seaweed <i>Ulva compressa</i> is a promising model for functional biology. In addition to historical research on growth and development, -omics data and molecular tools for stable transformation are available. However, more efficient tools are needed to study gene function.</li>\u0000<li>Here, we expand the molecular toolkit for <i>Ulva</i>.</li>\u0000<li>We screened the survival of <i>Ulva</i> and its mutualistic bacteria on 14 selective agents and established that Blasticidin deaminases (BSD or bsr) can be used as selectable markers to generate stable transgenic lines. We show that Cas9 and Cas12a RNPs are suitable for targeted mutagenesis and can generate genomic deletions of up to 20 kb using the marker gene <i>ADENINE PHOSPHORIBOSYLTRANSFERASE</i> (<i>APT</i>). We demonstrate that the targeted insertion of a selectable marker via homology-directed repair or co-editing with <i>APT</i> is possible for nonmarker genes. We evaluated 31 vector configurations and found that the bicistronic fusion of Cas9 to a resistance marker or the incorporation of introns in Cas9 led to the most mutants. We used this to generate mutants in three nonmarker genes using a co-editing strategy.</li>\u0000<li>This expanded molecular toolkit now enables us to reliably make gain- and loss-of-function mutants; additional optimizations will be necessary to allow for vector-based multiplex genome editing in <i>Ulva</i>.</li>\u0000</ul><p></p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"204 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143627621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stomatal-based immunity differentiation across vascular plant lineages","authors":"Yuan-Yuan Zeng, Xu-Dong Liu, Guang-Qian Yao, Min-Hui Bi, Xiangling Fang, Kailiang Yu, Jinsheng He, Jianquan Liu, Timothy J. Brodribb, Xiang-Wen Fang","doi":"10.1111/nph.70077","DOIUrl":"https://doi.org/10.1111/nph.70077","url":null,"abstract":"<p>\u0000</p><ul>\u0000<li>Some plants are known to actively close their stomata in the presence of foliar pathogens, inhibiting pathogen entry into leaves, leading to ‘stoma-based immunity’ as the first line of defense. However, the variation in stoma-based innate immunity across the diversity of vascular plants remains unclear.</li>\u0000<li>Here, we investigated the stomatal response and guard cell signaling pathway in various seed plant, fern, and lycophyte species when exposed to the bacterial pathogens or pathogen-associated molecular patterns (PAMPs).</li>\u0000<li>We observed active stomatal closure in 10 seed plants when exposed to bacteria or PAMPs, whereas none of the nine fern and one lycophyte species exhibited this response. The PAMP flg22-induced reactive oxygen species burst was observed in all species, but the downstream signaling events, including cytosolic Ca²⁺ accumulation, nitric oxide production, ion fluxes, vacuolar acidification, cytoplasmic pH elevation, vacuolar compartmentation, and disaggregation of the actin cytoskeleton in guard cells, were only observed in seed plants. No such changes were observed in the representatives of ferns and lycophytes.</li>\u0000<li>Our findings suggest a major difference in the regulation of stomatal immunity between seed plants and ferns and lycophytes under this study's conditions, unveiling physiological and biophysical mechanisms that may have underpinned the evolutionary adaptation of stomatal responses to pathogen attacks in seed plants.</li>\u0000</ul><p></p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"23 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143627471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Defining the pyro-thermal niche: do seed traits, ecosystem type and phylogeny influence thermal thresholds in seeds with physical dormancy?","authors":"Ryan Tangney, Sarah J. McInnes, Emma L. Dalziell, William K. Cornwell, Ben P. Miller, Tony D. Auld, Mark K. J. Ooi","doi":"10.1111/nph.70061","DOIUrl":"https://doi.org/10.1111/nph.70061","url":null,"abstract":"&lt;h2&gt; Introduction&lt;/h2&gt;\u0000&lt;p&gt;Regeneration of plants from seeds is fundamental for species persistence and population expansion (Nolan &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2021&lt;/span&gt;). Seeds are one of the key pathways for plant populations to recover following disturbances and are essential both for dispersal and as a means for adaptation to environmental conditions (Baskin &amp; Baskin, &lt;span&gt;2001&lt;/span&gt;). At a population level, seed dormancy enables the formation of soil seed banks, allowing for the emergence of seedlings in response to ecosystem disturbances, including fire (Baskin &amp; Baskin, &lt;span&gt;2001&lt;/span&gt;; Penfield, &lt;span&gt;2017&lt;/span&gt;). Seed dormancy is a critical mechanism for species persistence in ecosystems that experience sporadic ecological disturbances, for example fire, as it ensures seeds germinate at the most opportune time to maximise recruitment success (Ooi &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2022&lt;/span&gt;). Understanding how seed traits and dormancy mechanisms differ between species can allow us to predict species responses to disturbances such as fire (Saatkamp &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2019&lt;/span&gt;), which are projected to become more frequent and severe under global climate change (Boer &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2016&lt;/span&gt;).&lt;/p&gt;\u0000&lt;p&gt;Seed dormancy is common amongst vascular plants, occurring in &gt; 50% of all wild species globally (Baskin &amp; Baskin, &lt;span&gt;2001&lt;/span&gt;; Kildisheva &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2020&lt;/span&gt;). Seeds with physical dormancy (PY) are released from the mother plant with a water-impermeable seed coat which restricts their ability to hydrate and thus germinate (Baskin &amp; Baskin, &lt;span&gt;2004&lt;/span&gt;), but once the seed coat is ruptured, hydration can occur, and germination will proceed given suitable moisture and temperature conditions. Physical dormancy is a derived trait, having evolved relatively recently (Willis &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2014&lt;/span&gt;), and is known to occur in at least 18 families of angiosperms (Baskin &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2000&lt;/span&gt;, &lt;span&gt;2006&lt;/span&gt;), including Fabaceae, Rhamnaceae, Sapindaceae and Malvaceae (Baskin, &lt;span&gt;2003&lt;/span&gt;; Baskin &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2006&lt;/span&gt;). Dormancy loss in PY seeds represents a critical life stage transition for plants, as the process of dormancy break is often irreversible (Baskin &amp; Baskin, &lt;span&gt;2001&lt;/span&gt;) and usually results in seeds germinating quickly once hydrated (Ryan &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2023&lt;/span&gt;). Therefore, the mechanisms responsible for the alleviation of PY are fine-tuned to ensure germination occurs when seedling emergence and survival are most likely optimal for that population of seeds (Overton &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2024&lt;/span&gt;).&lt;/p&gt;\u0000&lt;p&gt;Seed dormancy is particularly common among species in fire-prone ecosystems, with germination cues aligned with fire-derived stimulants (Collette &amp; Ooi, &lt;span&gt;2021&lt;/span&gt;; Pausas &amp; Lamont, &lt;span&gt;2022&lt;/span&gt;), and fire promoting a pulse of seedling emergence for many species. Fire creates a unique window in time, providing condi","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"183 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143619037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The E3 ubiquitin ligase BRG3 and the protein kinase MPK7 antagonistically regulate LBD36 turnover, a key node for integrating nitrate and gibberellin signaling in apple","authors":"Xin-Long Guo, Da-Ru Wang, Baoyou Liu, Yuepeng Han, Chun-Xiang You, Jian-Ping An","doi":"10.1111/nph.70040","DOIUrl":"https://doi.org/10.1111/nph.70040","url":null,"abstract":"<p>\u0000</p><ul>\u0000<li>Nitrate is the main source of nitrogen in plants. Nitrate stimulation causes changes in plant secondary metabolites, including anthocyanins. However, the molecular mechanism underlying how nitrate regulates anthocyanin biosynthesis remains unclear. In this study, we identified a nitrate response factor MdLBD36 in apple. This factor positively regulated nitrate deficiency-induced anthocyanin biosynthesis by promoting the transcriptional activity of MdABI5, an important regulator of anthocyanins, and directly activated <i>MdABI5</i> expression.</li>\u0000<li>The E3 ubiquitin ligase MdBRG3 promoted the ubiquitinated degradation of MdLBD36 to reduce anthocyanin biosynthesis under nitrate-sufficient conditions. Nitrate deficiency-activated MdMPK7 maintained the stimulating effect of MdLBD36 on anthocyanin biosynthesis by counteracting the MdBRG3-mediated degradation of MdLBD36.</li>\u0000<li>Nitrate coordinated gibberellin (GA) signaling to regulate anthocyanin biosynthesis. The GA signaling repressor MdRGL2a contributed to MdLBD36-promoted anthocyanin biosynthesis by enhancing the MdLBD36–MdABI5 interaction and increasing the MdLBD36 transcriptional activation of <i>MdABI5</i>.</li>\u0000<li>In summary, our results elucidate the molecular framework of the coordinated regulation of the nitrate signaling response and anthocyanin biosynthesis by ubiquitination and phosphorylation. This study revealed the cross talk between nitrate and GA signaling in the regulation of anthocyanin biosynthesis and provides references for an in-depth exploration of the nitrate signal transduction pathway and its interactions with hormones.</li>\u0000</ul><p></p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"213 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143619036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular and physiological characterization of brassinosteroid receptor BRI1 mutants in Sorghum bicolor","authors":"Andrés Rico-Medina, Natalie Laibach, Juan B. Fontanet-Manzaneque, David Blasco-Escámez, Fidel Lozano-Elena, Damiano Martignago, Ana I. Caño-Delgado","doi":"10.1111/nph.20443","DOIUrl":"https://doi.org/10.1111/nph.20443","url":null,"abstract":"<p>\u0000</p><ul>\u0000<li>The high sequence and structural similarities between BRASSINOSTEROID INSENSITIVE 1 (BRI1) brassinosteroid (BR) receptors of Arabidopsis (<i>AtBRI1</i>) and sorghum (<i>SbBRI1</i>) prompted us to study the functionally conserved roles of BRI1 in both organisms.</li>\u0000<li>Introducing sorghum SbBRI1 in Arabidopsis <i>bri1</i> mutants restores defective growth and developmental phenotypes to wild-type levels.</li>\u0000<li>Sorghum mutants for <i>SbBRI1</i> show defective BR sensitivity and impaired plant growth and development throughout the entire sorghum life cycle. Embryonic analysis of sorghum primary root techniques permits to trace back root growth and development to early stages in an unprecedented way, revealing the functionally conserved roles of the SbBRI1 receptor in BR perception during meristem development. RNA-seq analysis uncovers the downstream regulation of the <i>SbBRI1</i> pathway in cell wall biogenesis during cell growth.</li>\u0000<li>Together, these results uncover that the sorghum SbBRI1 protein plays functionally conserved roles in plant growth and development, while encouraging the study of BR pathways in sorghum and its implications for improving resilience in cereal crops.</li>\u0000</ul><p></p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"1 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143608694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elevated CO₂: a double-edged sword for plant defence against pathogens","authors":"Rosa Sanchez-Lucas, Estrella Luna","doi":"10.1111/nph.70048","DOIUrl":"https://doi.org/10.1111/nph.70048","url":null,"abstract":"<p>Human activities have caused atmospheric carbon dioxide (CO₂) concentrations to double since the onset of the industrial era, with levels continuing to rise at an unprecedented rate. This dramatic increase has far-reaching consequences for plant physiology, ecosystems, and global agriculture. While rising CO₂ may enhance plant growth through the well-known ‘CO₂ fertilisation’ effect (Ainsworth &amp; Long, <span>2021</span>), its influence on plant health is far more complex. Recent research has demonstrated that elevated CO₂ (eCO₂) can alter plant immune responses to pathogens, with highly variable outcomes. Reports indicate that eCO₂ can enhance, suppress or have no effect on plant resistance, depending on the specific host–pathogen interactions, environmental conditions, and pathogen lifestyles (Bazinet <i>et al</i>., <span>2022</span>; Li &amp; Ahammed, <span>2023</span>; Sanchez-Lucas <i>et al</i>., <span>2023</span>; Smith &amp; Luna, <span>2023</span>). This variability underscores the need to better understand the interplay between eCO₂ and plant immunity to predict the potential impacts of climate change on plant health. Plant diseases, caused by a wide range of pathogens, are among the most significant threats to global food security, biodiversity, and forest health. The spread and virulence of plant pathogens are already being shaped by climate change, making it critical to investigate how rising CO₂ interacts with plant defence mechanisms. The study by Bredow <i>et al</i>. recently published in <i>New Phytologist</i> (<span>2025</span>; doi: 10.1111/nph.20364) takes an important step towards addressing this knowledge gap. By evaluating how eCO₂ affects soybean in response to three distinct pathogen lifestyles (biotrophic, hemibiotrophic, and necrotrophic), the research provides a valuable framework for understanding the contrasting effects of eCO₂ reported in previous studies. Crucially, Bredow <i>et al</i>. explore the influence of eCO₂ under a concentration of 550 ppm, which reflects realistic projections for atmospheric CO₂ levels by 2050 (Hamdan <i>et al</i>., <span>2023</span>). This approach enhances the relevance of their findings for understanding plant–pathogen dynamics in the near future, making their results directly applicable to ongoing discussions about the impacts of climate change on agriculture and ecosystems.</p>\u0000<div>\u0000<blockquote><p><i>Understanding the broader implications of eCO₂ on plant performance remains a significant challenge due to the contrasting effects reported across diverse studies.</i></p>\u0000<div></div>\u0000</blockquote>\u0000</div>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"81 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143608693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soybean RING-type E3 ligase GmCHYR16 ubiquitinates the GmERF71 transcription factor for degradation to negatively regulate bicarbonate stress tolerance","authors":"Tong Wu, Yan Wang, Jun Jin, Bingqian Zhao, Shengyang Wu, Bowei Jia, Xiaoli Sun, Dajian Zhang, Mingzhe Sun","doi":"10.1111/nph.70041","DOIUrl":"https://doi.org/10.1111/nph.70041","url":null,"abstract":"<p>\u0000</p><ul>\u0000<li>Plant AP2/ERF (APETALA2/ethylene response factor) transcription factors are key regulators of environmental stress tolerance. We previously characterized that the wild soybean ERF71 transcription factor conferred bicarbonate stress tolerance; however, the underlying mechanism still remains elusive.</li>\u0000<li>Here, multiple approaches were used to identify the E3 ubiquitin ligase GmCHYR16 as an interactor of GmERF71. Ubiquitination and protein degradation of GmERF71 mediated by GmCHYR16 were then analyzed. Overexpression transgenic lines were generated to evaluate the function of GmCHYR16 and GmERF71 in bicarbonate stress response.</li>\u0000<li>GmCHYR16 interacts with GmERF71. GmERF71 proteins undergo ubiquitination and 26S proteasome-mediated degradation, and GmCHYR16 mediates the ubiquitination of GmERF71 for degradation. The GmCHYR16-mediated ubiquitination and proteasome-dependent degradation of GmERF71 are reduced under bicarbonate stress. <i>GmCHYR16</i> expression in transgenic Arabidopsis, soybean hairy roots, and stable transgenic soybean reduces bicarbonate stress tolerance. GmERF71 degradation is decreased in the protein extracts of <i>atchyr1/7</i> mutants, and <i>atchyr1/7</i> mutants display higher bicarbonate tolerance. Overexpression of <i>GmERF71</i> in transgenic soybean obviously increases bicarbonate tolerance, and <i>GmCHYR16</i> reduces the bicarbonate tolerance of transgenic hairy root composite soybean plants by repressing GmERF71.</li>\u0000<li>Our results demonstrate that GmCHYR16 directly ubiquitinates GmERF71 for degradation and negatively regulates bicarbonate stress tolerance.</li>\u0000</ul><p></p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"1 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143608695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using long-term field data to quantify water potential regulation in response to VPD and soil moisture in a conifer tree","authors":"Ibrahim Bourbia, Luke A. Yates, Timothy J. Brodribb","doi":"10.1111/nph.70056","DOIUrl":"https://doi.org/10.1111/nph.70056","url":null,"abstract":"&lt;h2&gt; Introduction&lt;/h2&gt;\u0000&lt;p&gt;As plants emerged from swamps and oceans onto the terrestrial earth, a fundamental new pressure came to dominate evolutionary selection in land plants. Water pressure, or more correctly, apoplastic water potential inside the walls of the plant body, is the tension that connects living cells in the plant to water in the soil. This tension enables plants to pull water passively from the soil, but it can also pose a lethal risk when unregulated by the plant. Decreasing soil moisture levels or high rates of transpiration caused by high vapour pressure deficit (VPD) or a combination of both force plant water potential to become more negative, increasing the water tension in the plant body towards damaging values. Under such conditions, the water column in the vascular system begins to break down in a process commonly called xylem cavitation, which occurs as air bubbles are pulled into xylem conduits where they expand, creating embolisms that block the xylem. Xylem embolism disconnects the plant from the soil and has been causally linked to leaf tissue damage and strongly correlated with plant death (Choat &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2018&lt;/span&gt;; Brodribb &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2021&lt;/span&gt;; McDowell &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2022&lt;/span&gt;).&lt;/p&gt;\u0000&lt;p&gt;Xylem embolism may be the most obvious damage linked to uncontrolled decline in plant water potential, but other symptoms of excessive xylem tension, such as tissue collapse in leaves (Zhang &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2016&lt;/span&gt;; Corso &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2020&lt;/span&gt;) and roots (North &amp; Nobel, &lt;span&gt;1991&lt;/span&gt;; Cuneo &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2016&lt;/span&gt;; Bourbia &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2021&lt;/span&gt;; Harrison Day &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2023&lt;/span&gt;), also present a clear selection pressure pushing plants to regulate their xylem water potential by the action of stomata. Stomatal pores on the leaf surface enable strong control of transpiration, providing the mechanism for plants to regulate water potential and to restrict soil dehydration. The stringency with which stomata control water potential has been suggested to vary between species and may be an important axis of variation in water use and survival strategy (Gilbert &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2011&lt;/span&gt;; Gholipoor &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2013&lt;/span&gt;; Choudhary &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2014&lt;/span&gt;; Cooper &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2014&lt;/span&gt;; Gleason &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2022&lt;/span&gt;). Because of its potential implications for plant performance and survival during drought, various metrics have emerged that attempt to classify plant species based on the degree of homeostasis in stem water potential (Ψ&lt;sub&gt;stem&lt;/sub&gt;) as soil dehydrates (declining soil water potential; Ψ&lt;sub&gt;soil&lt;/sub&gt;), such as hydroscape area (i.e. the water potential landscape over which stomata regulate Ψ&lt;sub&gt;stem&lt;/sub&gt;) and isohydricity (i.e. the slope of the linear relationship between midday and predawn Ψ&lt;sub&gt;stem&lt;/sub&gt; describing the stringency of stomatal control) (Martínez-Vilalta &lt;i&gt;et al&lt;/i&gt;., &lt;s","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"19 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143608692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing hyperaccumulator plants to recover technology-critical metals: where are we at?","authors":"Elizabeth L. Rylott, Antony van der Ent","doi":"10.1111/nph.20449","DOIUrl":"https://doi.org/10.1111/nph.20449","url":null,"abstract":"Since its inception over three decades ago, phytomining has finally reached the stage of commercial-scale implementation, at least for nickel. Much potential remains to be realised for other elements, notably cobalt, selenium, and thallium, but this requires scientific impetus leveraging recent advances in insights garnered from molecular mechanisms of hyperaccumulation, domestication and agronomic development. These advances will also enable us to (genetically) improve hyperaccumulators for use in phytomining by targeted breeding, as well as synthetic biology approaches.","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"31 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143599515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coordinated variations in leaf and root biogeochemical niches","authors":"Chao Wang, Zhihui Yang, Mingzhu He, Yu-Kun Hu, Yanbao Lei, Yanhui Hou, Ji Suonan, Yonghui Wang, Lingfei Yu, Josep Peñuelas, Jordi Sardans, Xiaona Li","doi":"10.1111/nph.70042","DOIUrl":"https://doi.org/10.1111/nph.70042","url":null,"abstract":"<p>\u0000</p><ul>\u0000<li>The biogeochemical niche (BN) hypothesis posits that each species has a specific elemental composition. However, the BN of roots and its interaction with leaf BN have largely been neglected until now across diverse environmental conditions.</li>\u0000<li>We investigated the relationships between the elemental compositions of leaves and roots, phylogeny, and environmental variables, as well as the connection between leaf and root BN. We analyzed the concentrations of carbon, nitrogen, phosphorus, potassium, calcium, and magnesium in the leaves and roots of 12 394 individuals from 1238 species.</li>\u0000<li>Consistent with the BN hypothesis, despite significant differences in elemental concentrations and their ratios between leaves and roots, we observed strong legacy (phylogenetic + species) signals in the species-specific elemental compositions. This finding confirms that the elemental compositions of leaves and roots can contribute to identifying species niches. Our study revealed a higher phylogenetic conservatism for BN in leaves than in roots and provided evidence of a tight association between the species-specific BN of leaves and roots.</li>\u0000<li>Our results underscore the broad applicability of the BN hypothesis across diverse species and biomes and demonstrate the critical role of evolutionary legacy in driving coordinated dynamics in both above- and belowground ecological niches.</li>\u0000</ul><p></p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"19 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143582540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}