New Phytologist最新文献_第6页

Low-nitrogen input enriches Massilia bacteria in the phyllosphere to improve blast resistance in rice. 低氮输入可丰富层层内的麦氏菌，提高水稻抗稻瘟病能力。

IF 9.4 1区生物学

New Phytologist Pub Date : 2025-09-18 DOI: 10.1111/nph.70582

Qiong Huang,Rongfeng Wang,Qihui Ding,Fang Liao,Linxing Zhu,Mengting Huang,Jia Li,Jixing Zeng,Qirong Shen,Min Wang,Shiwei Guo

{"title":"Low-nitrogen input enriches Massilia bacteria in the phyllosphere to improve blast resistance in rice.","authors":"Qiong Huang,Rongfeng Wang,Qihui Ding,Fang Liao,Linxing Zhu,Mengting Huang,Jia Li,Jixing Zeng,Qirong Shen,Min Wang,Shiwei Guo","doi":"10.1111/nph.70582","DOIUrl":"https://doi.org/10.1111/nph.70582","url":null,"abstract":"Excessive nitrogen (N) fertilization exacerbates rice blast disease caused by Magnaporthe oryzae, yet the underlying microbiological mechanisms remain unclear. Through integrated field surveys and controlled experiments, we demonstrate that high-N application worsens disease severity by disrupting microbiome communities in the phyllosphere of rice (Oryza sativa) plants. We identified microorganisms in the phyllosphere through 16S rDNA sequencing. Low-N conditions were associated with a greater Massilia bacterial population, which was negatively correlated with pathogen abundance and disease index. In vitro and in vivo experiments indicated that Massilia enhances rice resistance to rice blast disease. We explored how Massilia confers resistance. Massilia strain z571 directly inhibited M. oryzae spore germination and hyphal invasion while also activating host immunity, including elevated levels of salicylic acid and jasmonic acid, enhanced expression of defense-related genes, and increased activity of antioxidant defense enzymes. Moreover, Massilia exhibited broad-spectrum antagonism against multiple pathogens. Our findings unveil a microbiome-mediated mechanism linking N fertilization to disease susceptibility and highlight Massilia as a potential biocontrol agent, offering a sustainable strategy for managing rice blast through optimized fertilization practices and microbial interventions.","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"1 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145078201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing the benefits of herbarium specimen digitisation for inferring recent and ongoing plant extinctions. 利用植物标本馆标本数字化的好处来推断最近和正在进行的植物灭绝。

IF 9.4 1区生物学

New Phytologist Pub Date : 2025-09-18 DOI: 10.1111/nph.70552

Aelys M Humphreys,Diana O Fisher,Naomi A Witts,Daniele Silvestro,Alexandre Antonelli

{"title":"Harnessing the benefits of herbarium specimen digitisation for inferring recent and ongoing plant extinctions.","authors":"Aelys M Humphreys,Diana O Fisher,Naomi A Witts,Daniele Silvestro,Alexandre Antonelli","doi":"10.1111/nph.70552","DOIUrl":"https://doi.org/10.1111/nph.70552","url":null,"abstract":"Evidence for the ongoing biodiversity crisis rests on assessment of a small fraction of described species, with major knowledge gaps for most organisms, including plants. Here, we highlight how digitised herbarium specimens can be used to accelerate and improve estimates of recent and ongoing plant extinctions. We focus on species already considered extinct because they represent a special category for understanding biodiversity loss and a special scientific challenge, as their 'detection' relies on proving absence. We propose that this challenge is embodied by a neglected biodiversity shortfall, the Katuš shortfall, encompassing all facets of unquantified levels of past, present and future biodiversity loss. To address this shortfall, we review how methods for estimating the probability of species extinction can be scaled up to harness the massive amounts of digitised data being produced across the 'global metaherbarium' using artificial intelligence. Thus, we suggest that the Katuš shortfall can be diminished by shifting focus from proving absence to a probabilistic framework. This can contribute to increasing the accuracy of lists of extinct plants and reveal the true extent of the biodiversity crisis.","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"119 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145078200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multifunctional NAC transcription factor SlJA2L integrates the ethylene pathway to orchestrate thermotolerance and fruit ripening in tomato. 多功能NAC转录因子SlJA2L整合乙烯途径调控番茄的耐热性和果实成熟。

IF 9.4 1区生物学

New Phytologist Pub Date : 2025-09-18 DOI: 10.1111/nph.70571

Yunfei Liang,Fang Ma,Shudi Huang,Chao Ma,Hongyan Shi,Fanyi Meng,Yinglu Chen,Han Dong,Rugang Chen,Xiangqiang Zhan

{"title":"Multifunctional NAC transcription factor SlJA2L integrates the ethylene pathway to orchestrate thermotolerance and fruit ripening in tomato.","authors":"Yunfei Liang,Fang Ma,Shudi Huang,Chao Ma,Hongyan Shi,Fanyi Meng,Yinglu Chen,Han Dong,Rugang Chen,Xiangqiang Zhan","doi":"10.1111/nph.70571","DOIUrl":"https://doi.org/10.1111/nph.70571","url":null,"abstract":"High temperatures driven by climate change are increasingly limiting crop growth and quality. Fruit ripening is a genetically programmed process regulated by transcription factors (TFs), while the phytohormone ethylene plays key roles in both ripening and abiotic stress responses. However, the underlying regulatory mechanisms remain poorly understood. We identified Solanum lycopersicum jasmonic acid 2-like (SlJA2L), a tomato NAC TF that is transcriptionally responsive to both heat stress (HS) and fruit ripening, suggesting that it acts as a molecular integrator of HS adaptation and fruit developmental processes. Overexpression of SlJA2L enhanced heat tolerance and promoted fruit ripening, whereas clustered regularly interspaced short palindromic repeats-CRISPR-associated protein 9 (CRISPR-Cas9)-mediated knockout led to heat sensitivity and ripening delay. Mechanistically, SlJA2L directly activates SlHsfA3 and SlCAT3 under HS. During ripening, it promotes ethylene biosynthesis via the SlJA2L-SlACO1 module and enhances carotenoid accumulation by activating SlCRTISO. Moreover, ethylene was shown to contribute to thermotolerance, as exogenous 1-aminocyclopropane-1-carboxylic acid improved heat tolerance, while silencing SlACO1 reduced it. Our findings demonstrate that SlJA2L integrates ethylene signaling to regulate both thermotolerance and fruit ripening in tomato. This study provides new insights into the molecular coordination of stress responses and developmental transitions, offering a potential strategy for improving crop resilience and fruit ripening under climate stress.","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"73 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145078202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The path toward a unified trait space: synthesizing plant functional diversity. 迈向统一性状空间之路：综合植物功能多样性。

IF 9.4 1区生物学

New Phytologist Pub Date : 2025-09-18 DOI: 10.1111/nph.70584

Carlos P Carmona,Eleonora Beccari

引用次数: 0

Modulation of SAL retrograde signalling promotes yield and water productivity responses in dynamic field environments. SAL逆行信号的调制促进了动态田间环境下的产量和水分生产力响应。

IF 9.4 1区生物学

New Phytologist Pub Date : 2025-09-15 DOI: 10.1111/nph.70579

Andrew F Bowerman,Marten Moore,Arun Yadav,Jing Zhang,Matthew D Mortimer,Zuzana Plšková,Estee E Tee,Eng Kee Au,Derek P Collinge,Gonzalo M Estavillo,Crispin A Howitt,Kai X Chan,Greg J Rebetzke,Barry J Pogson

{"title":"Modulation of SAL retrograde signalling promotes yield and water productivity responses in dynamic field environments.","authors":"Andrew F Bowerman,Marten Moore,Arun Yadav,Jing Zhang,Matthew D Mortimer,Zuzana Plšková,Estee E Tee,Eng Kee Au,Derek P Collinge,Gonzalo M Estavillo,Crispin A Howitt,Kai X Chan,Greg J Rebetzke,Barry J Pogson","doi":"10.1111/nph.70579","DOIUrl":"https://doi.org/10.1111/nph.70579","url":null,"abstract":"Chloroplast-to-nucleus retrograde signalling enables rapid stress responses in plants, but whether these signals accumulate to affect crop performance across entire growing seasons under field conditions remains unknown. We generated wheat mutants with targeted deletions in specific SAL gene copies from two distinct homeologous groups (TaSAL1 and TaSAL2), creating lines with enhanced stress signal responsiveness. We tested these lines across 15 field trials spanning diverse Australian environments with varying temperatures, rainfall, and irrigation regimes, measuring physiological responses, yield, biomass, and water productivity. Lines with TaSAL2 gene deletions showed 4-8% yield improvements with enhanced water productivity, while TaSAL1 deletions reduced yields. The TaSAL2 mutants maintained superior photosynthetic function under drought stress, showed improved relative water content, and demonstrated enhanced yield stability across environments. Canopy temperature measurements revealed dynamic stomatal regulation, with increased closure during midday stress periods but normal aperture under benign conditions. Significantly, specific SAL modifications enhanced photosynthetic efficiency and stress resilience without traditional yield penalties. Targeted modification of specific SAL homeologous groups can simultaneously improve both yield and stress tolerance in wheat. This demonstrates that retrograde signalling integrates environmental information across the plant lifecycle and highlights the importance of locus-specific targeting and multienvironment field validation for crop modifications.","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"28 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145059217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methylome and transcriptome landscapes reveal Tetraspanin8 as a key player of cold tolerance in Citrus. 甲基组和转录组分析表明，Tetraspanin8是柑橘抗寒性的关键基因。

IF 9.4 1区生物学

New Phytologist Pub Date : 2025-09-15 DOI: 10.1111/nph.70464

Yilei Wang,Xin Jiang,Yue Wang,Jing Qu,Peng Xiao,Wei Xiao,Yike Zeng,Xi Zeng,Tian Fang,Xiangming Shang,Haowei Chen,Chunlong Li,Ji-Hong Liu

{"title":"Methylome and transcriptome landscapes reveal Tetraspanin8 as a key player of cold tolerance in Citrus.","authors":"Yilei Wang,Xin Jiang,Yue Wang,Jing Qu,Peng Xiao,Wei Xiao,Yike Zeng,Xi Zeng,Tian Fang,Xiangming Shang,Haowei Chen,Chunlong Li,Ji-Hong Liu","doi":"10.1111/nph.70464","DOIUrl":"https://doi.org/10.1111/nph.70464","url":null,"abstract":"DNA methylation is a critical epigenetic mark regulating a diversity of physiological and biological processes in plants. However, the role of DNA methylation in the regulation of cold stress response remains poorly elucidated, particularly in perennial plants like citrus. In this study, 33 genes exhibited greater upregulation but more pronounced hypomethylation in Ichang papeda (Citrus ichangensis), a cold-hardy plant, relative to lemon (Citrus limon, cold-sensitive) by an integrated analysis of transcriptome and methylome. Tetraspanin8 (TET8) exhibited the most substantial changes in both expression and methylation levels. TET8, localized in the plasma membrane and endoplasmic reticulum, played a positive role in cold tolerance. TET8 was mainly methylated in the context of CpG in the exon and 3' untranslated region. 5-Azacytidine treatment led to noticeable inhibition of CpG methylation in TET8, significant upregulation of TET8 and enhanced cold tolerance. Moreover, the expression level of Methyltransferase1 (MET1) was lower in Ichang papeda than in lemon. Silencing of MET1 suppressed CpG methylation, but upregulated TET8 and conferred cold tolerance. These findings indicate that MET1-mediated CpG methylation in TET8 plays a key role in the modulation of cold tolerance, which provides valuable insights into the understanding on epigenetic regulation of cold stress response in plants.","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"41 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145059010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Co-occurrence of chloroplastic ROS production and salicylic acid induction in plant immunity. 叶绿体活性氧产生与水杨酸诱导在植物免疫中的共现。

IF 9.4 1区生物学

New Phytologist Pub Date : 2025-09-15 DOI: 10.1111/nph.70569

Charles Roussin-Léveillée,Méliane St-Amand,Philippe Desbiens-Fortin,Rosaëlle Perreault,Antoine Pelletier,Sabrina Gauthier,Faye Gaudreault-Lafleur,Isabelle Laforest-Lapointe,Peter Moffett

{"title":"Co-occurrence of chloroplastic ROS production and salicylic acid induction in plant immunity.","authors":"Charles Roussin-Léveillée,Méliane St-Amand,Philippe Desbiens-Fortin,Rosaëlle Perreault,Antoine Pelletier,Sabrina Gauthier,Faye Gaudreault-Lafleur,Isabelle Laforest-Lapointe,Peter Moffett","doi":"10.1111/nph.70569","DOIUrl":"https://doi.org/10.1111/nph.70569","url":null,"abstract":"Chloroplasts are important sites of metabolite biosynthesis, including precursors of the defense-related phytohormone salicylic acid (SA). Chloroplastic reactive oxygen species (cROS) have previously been shown to contribute significantly to disease resistance in Arabidopsis thaliana L. (Heyn). To understand the function of cROS in plant immunity, we coupled confocal cROS imaging with gene expression, mass spectrometry, and hyperspectral imaging analysis. Here, we show that cROS production correlates with the onset of SA biosynthesis and that abrogating cROS production during immune elicitation prevents the initiation of SA-dependent, but not SA-independent, immune responses. Furthermore, we show that the Pseudomonas syringae effector proteins HopM1 and AvrE1 suppress cROS production, contributing to virulence by facilitating the induction of water-soaking lesions through immune inhibition. We propose that certain ROS produced in the chloroplasts serve as triggers for the induction of the SA signaling cascade in plants and that they are targeted by a conserved family of pathogen effector proteins.","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"63 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145059225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strigolactone signaling repressor SMXL7 modulates genome architecture via JMJ30-mediated H3K27me3 demethylation in Arabidopsis. 拟南芥中独角麦内酯信号抑制因子SMXL7通过jmj30介导的H3K27me3去甲基化调节基因组结构。

IF 9.4 1区生物学

New Phytologist Pub Date : 2025-09-15 DOI: 10.1111/nph.70580

Jindong Li,Gang Xu,Guangfeng Qu,Huihui Ma,Zhiyao He,Mingyu Sun,Lu Gao,Haiyang Yu,Yuehua Wang,Yunwei Zhang,Meng Zhang,Li Chen,Ruifeng Yao

引用次数: 0

Physical constraints and environmental factors shape phloem anatomical traits in woody angiosperm species. 物理约束和环境因素影响木本被子植物韧皮部解剖特征。

IF 9.4 1区生物学

New Phytologist Pub Date : 2025-09-15 DOI: 10.1111/nph.70578

Yan Wang,Johannes Liesche,Alan Crivellaro,Jiří Doležal,Jan Altman,Donato Chiatante,Anastazija Dimitrova,Zexin Fan,Peili Fu,Félix Forest,Jozica Gričar,Patrick Heuret,Sandrine Isnard,Olivier Maurin,Antonio Montagnoli,Cyrille B K Rathgeber,Enkhchimeg Tsedensodnom,Santiago Trueba,Yann Salmon

{"title":"Physical constraints and environmental factors shape phloem anatomical traits in woody angiosperm species.","authors":"Yan Wang,Johannes Liesche,Alan Crivellaro,Jiří Doležal,Jan Altman,Donato Chiatante,Anastazija Dimitrova,Zexin Fan,Peili Fu,Félix Forest,Jozica Gričar,Patrick Heuret,Sandrine Isnard,Olivier Maurin,Antonio Montagnoli,Cyrille B K Rathgeber,Enkhchimeg Tsedensodnom,Santiago Trueba,Yann Salmon","doi":"10.1111/nph.70578","DOIUrl":"https://doi.org/10.1111/nph.70578","url":null,"abstract":"Xylem trait studies have enhanced our understanding of how plants strategically adapt their morphological and anatomical features to diverse climates. Despite the importance of the phloem in plant functioning, similar studies of phloem traits are lacking. To tackle this knowledge gap, we analyzed phloem anatomical traits of woody angiosperm species in relation to climate and the distance of samples to the stem tip. We collected main stem or branch cross-sections of 188 angiosperm woody species, which represent a wide range of climates and diverse families. Measurements of xylem vessel and phloem sieve element diameter, density, and lumen fraction were used in phylogenetic structural equation models to disentangle internal and climatic constraints on their morphological and anatomical features. Our results showed that distance-to-tip mainly affects sieve element and vessel diameter and density, while climate more strongly influenced conduit lumen fraction. Vessel size was positively correlated with temperature after correcting for the distance-to-tip, while sieve element diameter was correlated with water availability. Our results highlight the need to account for distance-to-tip when accessing anatomical variations linked to the environment, and show that sieve element traits respond to other climatic drivers than vessel traits rather than simply mirroring them.","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"24 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145068333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arabidopsis thaliana exhibits wide within-species variation in tolerance to boron limitation and root and shoot trait resilience associate with a pleiotropic locus. 拟南芥（Arabidopsis thaliana）对硼限制的耐受性和根、梢性状的恢复力在种内具有广泛的差异，这与多效性位点有关。

IF 9.4 1区生物学

New Phytologist Pub Date : 2025-09-15 DOI: 10.1111/nph.70570

Thomas D Alcock,Manuela Désirée Bienert,Astrid Junker,Rhonda C Meyer,Henning Tschiersch,Sreelekha Kudamala,Nicolaus von Wirén,Thomas Altmann,Gerd Patrick Bienert

{"title":"Arabidopsis thaliana exhibits wide within-species variation in tolerance to boron limitation and root and shoot trait resilience associate with a pleiotropic locus.","authors":"Thomas D Alcock,Manuela Désirée Bienert,Astrid Junker,Rhonda C Meyer,Henning Tschiersch,Sreelekha Kudamala,Nicolaus von Wirén,Thomas Altmann,Gerd Patrick Bienert","doi":"10.1111/nph.70570","DOIUrl":"https://doi.org/10.1111/nph.70570","url":null,"abstract":"To improve plant tolerance to suboptimal availability of the micronutrient boron (B), it is crucial to understand the mechanisms plants have evolved to tolerate B-limited conditions. We assessed temporal physiological, ionomic and molecular responses to B deficiency across 185 Arabidopsis thaliana accessions grown in soil-substrate in an automated phenotyping system and on agar plates. Whilst profound shoot- and root-growth inhibition was observed in most accessions under B limitation, seven highly B-deficiency tolerant accessions with < 20% reduced fresh and digital biomass accumulation were identified. Boron-efficient accessions were characterised by sustaining lateral more than primary root growth under B limitation. Whilst expression of B transporters increased under B limitation, no correlations between expression and B uptake or B efficiency were observed, suggesting increased B-use efficiency in B-efficient accessions. Phylogenetic analysis suggests B efficiency evolved independently multiple times in response to local environmental needs. Genome-wide association analyses identified a QTL on chromosome 4 that is associated with both root and shoot resilience to B limitation. Our results suggest that an optimised root system contributes to maintaining shoot productivity in B-limited conditions. Further dissection of the identified QTL and candidate genes will form an important strategy for elucidating the molecular control of B efficiency.","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"17 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145068334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0