New Phytologist最新文献

{"title":"MYB47 delays leaf senescence by modulating jasmonate pathway via direct regulation of CYP94B3/CYP94C1 expression in Arabidopsis","authors":"Jie Cao, Qi Yang, Yaning Zhao, Shuya Tan, Shichun Li, Dawei Cheng, Ruxue Zhang, Murao Zhang, Zhonghai Li","doi":"10.1111/nph.70133","DOIUrl":"https://doi.org/10.1111/nph.70133","url":null,"abstract":"Summary<jats:list list-type=\"bullet\"> <jats:list-item>Leaf senescence is a complex genetic process intricately regulated by multiple layers of control. Transcription factors, as master regulators of gene expression, play crucial roles in initiating and progressing leaf senescence.</jats:list-item> <jats:list-item>Through screening an activation‐tagged mutant library, we identified MYB47 as a negative regulator of leaf senescence. Constitutive or inducible overexpression of <jats:italic>MYB47</jats:italic> significantly delays leaf senescence, while loss‐of‐function mutants exhibit accelerated senescence. Transcriptome analysis revealed a marked suppression of jasmonic acid (JA) signaling in <jats:italic>MYB47</jats:italic> overexpression lines. Conversely, the <jats:italic>myb47</jats:italic> mutants display elevated JA levels and reduced expression of JA catabolic genes, <jats:italic>CYP94B3</jats:italic> and <jats:italic>CYP94C1</jats:italic>.</jats:list-item> <jats:list-item>Biochemical evidence demonstrated that MYB47 directly binds to the promoters of <jats:italic>CYP94B3</jats:italic> and <jats:italic>CYP94C1</jats:italic>, upregulating their expression. Consequently, JA contents are significantly reduced in <jats:italic>MYB47</jats:italic> overexpression lines. Overexpressing <jats:italic>CYP94B3</jats:italic> or <jats:italic>CYP94C1</jats:italic> in <jats:italic>myb47</jats:italic> mutants alleviates their early senescence phenotype. Furthermore, JA induces <jats:italic>MYB47</jats:italic> expression, forming a negative feedback loop (JA‐MYB47‐CYP94B3/C1‐JA) that fine‐tunes leaf senescence.</jats:list-item> <jats:list-item>Our findings reveal a novel regulatory module involving MYB47 and JA signaling that governs leaf senescence. By stimulating JA catabolism and attenuating JA signaling, MYB47 plays a crucial role in delaying leaf senescence.</jats:list-item> </jats:list>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"20 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143784675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non‐photochemical quenching (NPQ) in photoprotection: insights into NPQ levels required to avoid photoinactivation and photoinhibition","authors":"Guanqiang Zuo","doi":"10.1111/nph.70121","DOIUrl":"https://doi.org/10.1111/nph.70121","url":null,"abstract":"SummaryPlant photosynthesis is highly responsive to fluctuations in environmental cues. To achieve optimal photosynthetic performance, plants must accurately regulate light absorption, maintaining a dynamic balance between energy supply and consumption in the field. Understanding the potential damage and imbalances caused by excessive light during photosynthesis necessitates a comprehensive insight into the protective role of non‐photochemical quenching (NPQ). This rapid photoprotective mechanism dissipates excess excitation energy as heat and is ubiquitous throughout the plant kingdom. Previous reviews have primarily focused on the regulation of NPQ amplitude, often overlooking its efficiency in photoprotection. This review outlines the significance, components, and mechanisms of NPQ, presenting fundamental equations that quantitatively describe both NPQ amplitude and its protective functions. I highlight the methodological approaches to quantify the NPQ levels necessary to prevent photoinactivation and photoinhibition, respectively. I conclude by identifying key open questions regarding NPQ and suggesting directions for future research.","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"183 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143782638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of array tomography to elucidate nuclear clustering architecture in giant-feeding cells induced by root-knot nematodes","authors":"François Orange, Sophie Pagnotta, Olivier Pierre, Janice de Almeida Engler","doi":"10.1111/nph.70066","DOIUrl":"https://doi.org/10.1111/nph.70066","url":null,"abstract":"<p>\u0000</p><ul>\u0000<li>Plant-parasitic nematodes like root-knot nematodes (RKN; <i>Meloidogyne</i> spp.) cause great losses in agriculture by inducing root swellings, named galls, in host roots disturbing plant growth and development. Previous two-dimensional studies using different microscopy techniques revealed the presence of numerous nuclear clusters in nematode-induced giant cells within galls.</li>\u0000<li>Here, we show in three dimensions (3D) that nuclear clustering occurring in giant cells is revealed to be much more complex, illustrating subclusters built of multiple nuclear lobes. These nuclear subclusters are unveiled to be interconnected and likely communicate via nucleotubes, highlighting the potential relevance of this nuclear transfer for disease. In addition, microtubules and microtubule organizing centers are profusely present between the densely packed nuclear lobes, suggesting that the cytoskeleton might be involved in anchoring nuclear clusters in giant cells.</li>\u0000<li>This study illustrates that it is possible to apply volume electron microscopy (EM) approaches such as array tomography (AT) to roots infected by nematodes using basic equipment found in most EM facilities. The application of AT was valuable to observe the cellular ultrastructure in 3D, revealing the remarkable nuclear architecture of giant cells in the model host <i>Arabidopsis thaliana</i>.</li>\u0000<li>The discovery of nucleotubes, as a unique component of nuclear clusters present in giant cells, can be potentially exploited as a novel strategy to develop alternative approaches for RKN control in crop species.</li>\u0000</ul><p></p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"23 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143784730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Out of site, out of mind? Considering pesticide drift and plant mutualisms","authors":"Charlie C. Nicholson","doi":"10.1111/nph.70135","DOIUrl":"https://doi.org/10.1111/nph.70135","url":null,"abstract":"","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"29 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143782637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circadian regulation of key physiological processes by the RITMO1 clock protein in the marine diatom Phaeodactylum tricornutum","authors":"Alessandro Manzotti,&nbsp;Raphaël Monteil,&nbsp;Soizic Cheminant Navarro,&nbsp;Dany Croteau,&nbsp;Lucie Charreton,&nbsp;Antoine Hoguin,&nbsp;Nils Fabian Strumpen,&nbsp;Denis Jallet,&nbsp;Fayza Daboussi,&nbsp;Peter G. Kroth,&nbsp;François-Yves Bouget,&nbsp;Marianne Jaubert,&nbsp;Benjamin Bailleul,&nbsp;Jean-Pierre Bouly,&nbsp;Angela Falciatore","doi":"10.1111/nph.70099","DOIUrl":"10.1111/nph.70099","url":null,"abstract":"<p>\u0000 </p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"246 4","pages":"1724-1739"},"PeriodicalIF":8.3,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/nph.70099","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143765456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phosphorylation of the strawberry MADS-box CMB1 regulates ripening via the catabolism of abscisic acid","authors":"Haoran Jia,&nbsp;Yanna Shi,&nbsp;Zhengrong Dai,&nbsp;Yunfan Sun,&nbsp;Xiu Shu,&nbsp;Baijun Li,&nbsp;Rongrong Wu,&nbsp;Shouzheng Lv,&nbsp;Jiahan Shou,&nbsp;Xiaofang Yang,&nbsp;Guihua Jiang,&nbsp;Yuchao Zhang,&nbsp;Andrew C. Allan,&nbsp;Kunsong Chen","doi":"10.1111/nph.70065","DOIUrl":"10.1111/nph.70065","url":null,"abstract":"<p>\u0000 </p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"246 4","pages":"1627-1646"},"PeriodicalIF":8.3,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/nph.70065","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143765493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sliding-window phylogenetic analyses uncover complex interplastomic recombination in the tropical Asian–American disjunct plant genus Hedyosmum (Chloranthaceae)","authors":"Peng-Wei Li, Yong-Bin Lu, Alexandre Antonelli, Zheng-Juan Zhu, Wei Wang, Xin-Mei Qin, Xue-Rong Yang, Qiang Zhang","doi":"10.1111/nph.70120","DOIUrl":"https://doi.org/10.1111/nph.70120","url":null,"abstract":"&lt;h2&gt; Introduction&lt;/h2&gt;\u0000&lt;p&gt;The chloroplast genomic (i.e. plastomic) sequences have long been used for inferring phylogenetic relationships of green plants. Current major plant classifications (e.g. Angiosperm Phylogeny Group classification, APG IV, &lt;span&gt;2016&lt;/span&gt;; Pteridophyte Phylogeny Group classification, PPG I, &lt;span&gt;2016&lt;/span&gt;) are predominantly based on the plastid phylogenies (Stull &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2023&lt;/span&gt;). Due to the rapid progress in DNA sequencing technologies along with decreasing costs, phylogenetic analyses using whole plastomes have become a routine practice (Wang &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2024&lt;/span&gt;). Plastomes have been presumed to be single double-stranded circular DNA molecules that are inherited uniparentally, with maternal inheritance in most angiosperms and paternal inheritance in gymnosperms (Birky, &lt;span&gt;1995&lt;/span&gt;; Dong &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2012&lt;/span&gt;; Greiner &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2015&lt;/span&gt;). These characteristics led to the general belief that plastomes are free from or less likely to undergo intermolecular recombination (Walker &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2019&lt;/span&gt;). Therefore, different plastomic genes or regions, which are assumed to share the same evolutionary trajectory, are often concatenated directly for phylogenetic analyses (Jansen &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2007&lt;/span&gt;; Moore &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2010&lt;/span&gt;; Li &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2021&lt;/span&gt;).&lt;/p&gt;\u0000&lt;p&gt;Despite the widespread use of plastomes in phylogenetics, both biparental inheritance and recombination of plastomes – processes that could inadvertently affect inference – have been increasingly detected. The mechanisms that maintain uniparental inheritance, including elimination or degradation of the organelle during male gametophyte development or after pollen mitosis or fertilization, may break down and lead to biparental inheritance (Nagata, &lt;span&gt;2010&lt;/span&gt;). Biparental inheritance of plastomes has been reported in some plant groups, such as &lt;i&gt;Passiflora&lt;/i&gt; (Passifloraceae; Hansen &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2007&lt;/span&gt;; Shrestha &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2021&lt;/span&gt;), &lt;i&gt;Cicer arietinum&lt;/i&gt; (Fabaceae; Kumari &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2011&lt;/span&gt;), and &lt;i&gt;Actinidia&lt;/i&gt; (Actinidiaceae; Li &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2013&lt;/span&gt;). It is believed that heteroplasmy, that is the mixture of different organelle genomes within a cell or individual, is widespread in both animals and plants (Nagata, &lt;span&gt;2010&lt;/span&gt;; Ramsey &amp; Mandel, &lt;span&gt;2019&lt;/span&gt;; Camus &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2022&lt;/span&gt;), and &lt;i&gt;c&lt;/i&gt;. 20% of angiosperm genera may have undergone biparental inheritance (Zhang &amp; Sodmergen., &lt;span&gt;2010&lt;/span&gt;; Sakamoto &amp; Takami, &lt;span&gt;2024&lt;/span&gt;). The biparental inheritance allows the coexistence of both maternal and paternal plastids in the same offspring cell, creating opportunities for interplastomic recombination. Interspecific plastomic recombination has been created and detected in experimental studies (Medgyesy &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;1985&lt;/span&gt;). However, unlike in","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"58 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143745309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogenetic relatedness predicts plant–plant nitrogen transfer better than the duration of water scarcity periods","authors":"Alicia Montesinos-Navarro,&nbsp;Sarah Collins,&nbsp;Cristina Dumitru,&nbsp;Miguel Verdú","doi":"10.1111/nph.70116","DOIUrl":"10.1111/nph.70116","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 </p><ul>\u0000 \u0000 <li>Intermittent water availability is a significant stress factor for plants, particularly in arid and semi-arid ecosystems. Plant nutrient demands often do not align with precipitation pulses that trigger nutrient mobilization and availability, but biotic interactions like plant facilitation (e.g. through nitrogen transfer among distant relatives) and mycorrhizal symbiosis may mitigate this asynchrony, enabling nutrient access despite temporal disparities.</li>\u0000 \u0000 <li>We conducted a field experiment with 324 plant individuals to test two hypotheses: (1) greater mycorrhizal fungi abundance increases the amount of ¹⁵N transferred between plants, particularly under conditions of fluctuating water availability, and (2) the amount of ¹⁵N transferred is affected by the phylogenetic relatedness between donor and receiver plants.</li>\u0000 \u0000 <li>We show that ¹⁵N transfer is prevalent in the studied semi-arid communities, occurring between all species pairs in 68% of the trials. Interestingly, we observed an increase in ¹⁵N transfer between distantly related species, and this phylogenetic pattern remained consistent across fungicide and water regime treatments, which did not affect ¹⁵N transfer.</li>\u0000 \u0000 <li>Elucidating the drivers of N transfer between plants under different environmental conditions can improve our predictions on how plant communities will respond to future climate challenges, especially prolonged droughts in Mediterranean ecosystems.</li>\u0000 </ul>\u0000 </div>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"246 4","pages":"1848-1860"},"PeriodicalIF":8.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143765495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The MYC2-JAMYB transcriptional cascade regulates rice resistance to brown planthoppers","authors":"Mengyu Liu,&nbsp;Huijing Li,&nbsp;Yumeng Chen,&nbsp;Zhixin Wu,&nbsp;Siwen Wu,&nbsp;Jing Zhang,&nbsp;Rui Sun,&nbsp;Yonggen Lou,&nbsp;Jing Lu,&nbsp;Ran Li","doi":"10.1111/nph.70059","DOIUrl":"10.1111/nph.70059","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 </p><ul>\u0000 \u0000 <li>Plant defense against herbivores is primarily regulated by the phytohormone jasmonate (JA). At the core, JA signaling is the MYC2 transcription factor (TF) that regulates the expression of an extensive array of defense-related genes. However, the regulatory mechanisms underlying MYC2-mediated herbivore resistance in rice are not fully understood.</li>\u0000 \u0000 <li>We employed brown planthopper (BPH) bioassays, transcriptional activation assays, transcriptome profiling, targeted metabolomics and cleavage under targets and tagmentation-sequencing analysis to investigate the biological function and regulatory mechanism of the JAMYB TF.</li>\u0000 \u0000 <li><i>JAMYB</i> is induced by BPH infestation and is transcriptionally regulated by MYC2. Mutations of <i>JAMYB</i> rendered rice plants susceptible to BPH attacks under laboratory and field conditions, indicating that JAMYB positively contributes to BPH resistance. BPH-elicited biosynthesis of phenolamides and volatile compounds was reduced in <i>jamyb</i> mutants compared with wild-type plants. These specialized metabolites, regulated by JAMYB, function as direct and indirect defenses to deter BPH damage or attract parasitoid wasps of BPH eggs. Furthermore, we found that JAMYB directly binds to AC motifs of key phenylpropanoid pathway genes and activates their expression, likely altering the metabolic flux for phenolamide biosynthesis.</li>\u0000 \u0000 <li>This study reveals the role of the MYC2-JAMYB module in JA-mediated rice resistance to BPH.</li>\u0000 </ul>\u0000 </div>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"246 4","pages":"1834-1847"},"PeriodicalIF":8.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143765499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Symplastic and apoplastic pathways for local distribution of silicon in rice leaves","authors":"Sheng Huang, Naoki Yamaji, Noriyuki Konishi, Namiki Mitani-Ueno, Jian Feng Ma","doi":"10.1111/nph.70110","DOIUrl":"https://doi.org/10.1111/nph.70110","url":null,"abstract":"&lt;h2&gt; Introduction&lt;/h2&gt;\u0000&lt;p&gt;Rice (&lt;i&gt;Oryza sativa&lt;/i&gt;) is a typical accumulating plant of silicon (Si), which is able to accumulate Si in the shoots up to 10% of dry weight (Ma &amp; Takahashi, &lt;span&gt;2002&lt;/span&gt;). This increase in accumulation is essential for high and stable production of rice (Tamai &amp; Ma, &lt;span&gt;2008&lt;/span&gt;). Silicon is actively absorbed by the roots in the form of silicic acid, a noncharged molecule (Ma &amp; Takahashi, &lt;span&gt;2002&lt;/span&gt;). After that, &gt; 95% of Si absorbed is immediately translocated to the aboveground parts, including the leaf sheath and blade, and husk. In these tissues, silicic acid is polymerized to silica via transpiration, which is deposited beneath the cuticle of leaves and inside particular cells of leaf epidermis, forming silica cells and silica bodies or silica bulliform cells (motor cells; Ma &amp; Takahashi, &lt;span&gt;2002&lt;/span&gt;). This deposition forms a mechanical barrier, which is important in protecting the plants from various stresses, such as pathogens, insect pests, drought, high salinity, metal toxicity, lodging, and nutrient imbalance stresses (Ma &amp; Takahashi, &lt;span&gt;2002&lt;/span&gt;).&lt;/p&gt;\u0000&lt;p&gt;To transport Si from soil solution to different organs and tissues, different transporters involved in uptake, root-to-shoot translocation, and distribution, at least, are required. During the last two decades, transporters involved in different transport steps have been identified in rice (Huang &amp; Ma, &lt;span&gt;2024&lt;/span&gt;). In terms of uptake, two transporters, including OsLsi1 and OsLsi2, have been identified. OsLsi1 belongs to the Nod26-like major intrinsic protein (NIP) subfamily of aquaporin-like proteins and functions as an influx transporter of Si (Ma &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2006&lt;/span&gt;), while OsLsi2 belongs to a putative anion transporter family without any similarity to OsLsi1 (Ma &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2007&lt;/span&gt;) and functions as an efflux transporter of Si. Both OsLsi1 and OsLsi2 are localized at the exodermis and endodermis in the mature root regions but show different polar localization. OsLsi1 is localized at the distal side, while OsLsi2 is localized at the proximal side (Ma &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2006&lt;/span&gt;; Yamaji &amp; Ma, &lt;span&gt;2007&lt;/span&gt;), forming an efficient uptake system for Si (Huang &amp; Ma, &lt;span&gt;2024&lt;/span&gt;). After uptake, Si as silicic acid is loaded into the root xylem by OsLsi3 (Huang &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2022&lt;/span&gt;), while it is unloaded from the xylem by OsLsi6 (Yamaji &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2008&lt;/span&gt;). OsLsi3, a homolog of OsLsi2, is localized at the root pericycle cells without polarity, while OsLsi6, a homolog of OsLsi1, is polarly localized at the adaxial side of the xylem parenchyma cells in leaf sheaths and leaf blades (Yamaji &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2008&lt;/span&gt;). Finally, the preferential distribution of Si to the husk is mediated by three different Si transporters: OsLsi6, OsLsi2, and OsLsi3, which are highly expressed in the nodes, especially in the node I (Yamaji &","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"20 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143745308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}