Revista Brasileira De Ciencias Farmaceuticas最新文献_第3页

Excreção de gordura fecal de ratos (Rattus norvegicus, Wistar), submetidos a dietas hiperlipídicas e hipercolesterolêmicas suplementadas com quitosana 添加壳聚糖的高脂肪和高胆固醇饮食对褐家鼠粪便脂肪的排泄

Revista Brasileira De Ciencias Farmaceuticas Pub Date : 2008-12-01 DOI: 10.1590/S1516-93322008000400017

A.D.R. Cherem, Adriana Bramosrki

{"title":"Excreção de gordura fecal de ratos (Rattus norvegicus, Wistar), submetidos a dietas hiperlipídicas e hipercolesterolêmicas suplementadas com quitosana","authors":"A.D.R. Cherem, Adriana Bramosrki","doi":"10.1590/S1516-93322008000400017","DOIUrl":"https://doi.org/10.1590/S1516-93322008000400017","url":null,"abstract":"The effect of two comercial brands of chitosan in the faecal fat excretion was investigated in mice submitted to hypercholesterolemic and hyperlipidic diets. 32 rats Wistar, recently weaned, distributed randomly in four groups (n=8): control group (CG), received diet of AIN93G; hyperlipidic group (HG): diet of AIN-93G modified in the lipids content (12%) and supplemented with cholesterol (1%); and the chitosan hyperlipidic groups 1 (QHG1) and chitosan hyperlipidic 2 (QHG2), that received the same diet of the HG, supplemented with their respective chitosan (5%). The faecal collection was conducted on the days 0, 14th and 28th of the experiment. The faecal fat determination (g) was conducted through the Soxhlet method. No significant statistical differences (p>0.05) were observed among the 4 groups as to the fat content in the beginning of the experiment. On the 14th day, the GHQ1 group had a significant increase of faecal fat when compared to the other three groups (p<0.05). On the 28th day, in the GHQ1 group, the faecal fat excretion was larger than in the other groups (p<0.05), while the group GHQ2 had not statistically differed from the group GH. The results suggest that the differences between these two groups can be caused by the purity degree, viscosity and deacetylation degree of the chitosans.","PeriodicalId":21193,"journal":{"name":"Revista Brasileira De Ciencias Farmaceuticas","volume":"27 1","pages":"701-706"},"PeriodicalIF":0.0,"publicationDate":"2008-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75053705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Análise da atividade antimicobacteriana de 36 extratos vegetais da Mata Atlântica brasileira 巴西大西洋森林36种植物提取物的抑菌活性分析

Revista Brasileira De Ciencias Farmaceuticas Pub Date : 2008-12-01 DOI: 10.1590/S1516-93322008000400013

Daniela Fernandes Ramos, Gilda Guimarães Leitão, Fernanda das Neves Costa, Lisandra Ferreira de Abreu, J. V. Villarreal, Suzana G. Leitão, Salvador Said Fernández, P. Silva

引用次数: 23

Validação de metodologia espectrofotométrica para quantificação dos flavonóides de Bauhinia cheilantha (Bongard) Steudel 紫荆花黄酮类化合物分光光度法定量方法的验证

Revista Brasileira De Ciencias Farmaceuticas Pub Date : 2008-12-01 DOI: 10.1590/S1516-93322008000400015

T. P. Sobrinho, C. E. D. Silva, J. Nascimento, Júlio Marcelino Monteiro, Ulysses Paulino de Albuquerque, E. L. C. D. Amorim

{"title":"Validação de metodologia espectrofotométrica para quantificação dos flavonóides de Bauhinia cheilantha (Bongard) Steudel","authors":"T. P. Sobrinho, C. E. D. Silva, J. Nascimento, Júlio Marcelino Monteiro, Ulysses Paulino de Albuquerque, E. L. C. D. Amorim","doi":"10.1590/S1516-93322008000400015","DOIUrl":"https://doi.org/10.1590/S1516-93322008000400015","url":null,"abstract":"Phytotherapeutic laboratories require access to methodologies that guarantee quality control for their products when these items are not already registered in pharmacopeias or official monographs. As such, the present study sought to validate analytical methodologies for quantifying natural flavonoids contained in extracts of \"pata-de-vaca\" (Bauhinia cheilantha [Bong.] Steudel) using visible light spectrophotometry and a rutin standard (µg/ml). All of the parameters required by ANVISA were evaluated. The specificity test revealed a maximum absorption peak at 420 nm. The methodology was considered linear and of high quantification sensitivity (2.51 µg/ml). The methodology also proved to be robust, and had high recovery levels (98.36%). The results obtained for repeatability, intermediate precision (intra- and inter-day), and reproducibility all certified the precision of the method, with values between 0.31 and 3.58%; the methodology was also considered exact (95.71-105.50%). This study demonstrated that the proposed methodology can be considered specific, precise, reproducible, exact, of low cost, and easy to perform.","PeriodicalId":21193,"journal":{"name":"Revista Brasileira De Ciencias Farmaceuticas","volume":"36 1","pages":"683-689"},"PeriodicalIF":0.0,"publicationDate":"2008-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90730391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 78

Estudo de utilização de medicamentos parenterais em uma unidade de internação pediátrica de um hospital universitário 某大学医院儿科住院病房肠外药物使用研究

Revista Brasileira De Ciencias Farmaceuticas Pub Date : 2008-12-01 DOI: 10.1590/S1516-93322008000400014

Maria Clara Porfirio de Souza, M. Goulart, V. Rosado, A. M. Reis

{"title":"Estudo de utilização de medicamentos parenterais em uma unidade de internação pediátrica de um hospital universitário","authors":"Maria Clara Porfirio de Souza, M. Goulart, V. Rosado, A. M. Reis","doi":"10.1590/S1516-93322008000400014","DOIUrl":"https://doi.org/10.1590/S1516-93322008000400014","url":null,"abstract":"A administracao de medicamentos parenterais tem grande importância na assistencia pediatrica e no risco para aquisicao de infeccoes hospitalares. Este estudo observacional transversal visou descrever a utilizacao de medicamentos em uma unidade de internacao pediatrica. Elaborou-se um instrumento de coleta de dados, as variaveis relativas a farmacoterapia foram coletadas da prescricao medica e a analise estatistica descritiva foi realizada no SPSS. A amostra foi constituida de 75 pacientes pediatricos, sendo 56,0 % do sexo masculino; apresentando como predominante a faixa etaria de lactentes. O tempo de internacao mais frequente foi maior que 20 dias (24,0%) seguida de 6 a 10 dias (21,3%) e menos de 3 dias (17,3%). A via parenteral foi prescrita para 56 pacientes (74,7%) e 19 (25,3%) utilizaram outras vias ou nao utilizaram medicamentos; sendo que a via parenteral endovenosa foi utilizada por 52 (92,9%) dos pacientes. O numero de medicamentos parenterais prescritos abrangeu 47 farmacos. A media do numero de medicamentos por paciente foi quatro, o que implica em exigencia de maior tempo da equipe de enfermagem em atividades relacionadas a administracao de medicamentos. O numero elevado de medicamentos prescritos desperta preocupacoes em relacao a seguranca. O farmaceutico deve estimular a conversao da via de administracao parenteral para a oral.","PeriodicalId":21193,"journal":{"name":"Revista Brasileira De Ciencias Farmaceuticas","volume":"41 1","pages":"675-682"},"PeriodicalIF":0.0,"publicationDate":"2008-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81550447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Avaliação da produção de óxido nítrico em ratos, submetidos aos exercícios aeróbio e anaeróbio 有氧和无氧运动大鼠一氧化氮产生的评价

Revista Brasileira De Ciencias Farmaceuticas Pub Date : 2008-12-01 DOI: 10.1590/S1516-93322008000400023

V. D'Ávila, Nozelmar Borges de Sousa Júnior, Fábio Borges de Sousa, Lídia Andreu Guillo

{"title":"Avaliação da produção de óxido nítrico em ratos, submetidos aos exercícios aeróbio e anaeróbio","authors":"V. D'Ávila, Nozelmar Borges de Sousa Júnior, Fábio Borges de Sousa, Lídia Andreu Guillo","doi":"10.1590/S1516-93322008000400023","DOIUrl":"https://doi.org/10.1590/S1516-93322008000400023","url":null,"abstract":"Nitric Oxide (NO) exerts important influences in several physiological processes. In this work we evaluated the production of sanguine NO in Wistar rats, submitted to the acute aerobic and anaerobic exercises. The formation of nitric oxide was verified through the dosage of the end products of oxidation of the metabolism of nitric oxide (nitrates). For this we used the colorimetric Griess method. We verified the existence of a significant difference (p = 0.000261) in the production of NO among the accomplishment of the aerobic swimming and the anaerobic, where the aerobic was shown more efficient in the promotion of higher levels. The acute aerobic exercise with duration of at least 10 minutes was shown more effective in the requirement production of NO in relation to the 5 minutes exercise. The positive relationship observed between the aerobic exercise and the formation of NO can help to explain the beneficial effects of the exercise in the cardiovascular health. We know that the practice of aerobic exercise and your duration increases the biodisponibility of NO, which is an important physiologic regulator of the blood pressure.","PeriodicalId":21193,"journal":{"name":"Revista Brasileira De Ciencias Farmaceuticas","volume":"20 1","pages":"755-761"},"PeriodicalIF":0.0,"publicationDate":"2008-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83931976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Efeito do beneficiamento sobre o valor nutricional do peixe mandim (Arius spixii) 加工对mandim鱼营养价值的影响

Revista Brasileira De Ciencias Farmaceuticas Pub Date : 2008-12-01 DOI: 10.1590/S1516-93322008000400012

Fabiana Rodrigues de Oliveira, G. M. Lira, E. Torres, R. Soares, Simone Mendonça, K. W. B. Silva, Sarah J. G. B. Simon, Tatiana Maria Palmeira dos Santos, Cyro Rego Cabral Júnior

引用次数: 9

Desenvolvimento e validação de método analítico para nistatina creme vaginal por cromatografia líquida de alta eficiência 高效液相色谱法制霉菌素阴道乳膏分析方法的建立与验证

Revista Brasileira De Ciencias Farmaceuticas Pub Date : 2008-12-01 DOI: 10.1590/S1516-93322008000400010

Zênia Maria Maciel Lavra, Fabiane Sônego, Rosali Maria Ferreira Silva, Flávia Patrícia Morais de Medeiros

{"title":"Desenvolvimento e validação de método analítico para nistatina creme vaginal por cromatografia líquida de alta eficiência","authors":"Zênia Maria Maciel Lavra, Fabiane Sônego, Rosali Maria Ferreira Silva, Flávia Patrícia Morais de Medeiros","doi":"10.1590/S1516-93322008000400010","DOIUrl":"https://doi.org/10.1590/S1516-93322008000400010","url":null,"abstract":"Nystatin is a polyenic antibiotic with fungistatic and fungicide characteristics that acts by de-structuring the cellular membrane of fungi and yeast. The nystatin vaginal cream is used for the treatment of vaginal candidiasis. Until recently, the official compendia professed the microbiological trial for dosing this antibiotic, method considered as non-feasible in the routine of quality control centers due to the excessive time for release of results. Aiming at obtaining an alternative method for dosing nystatin vaginal cream, a chromatographic method (HPLC) was developed and validated. The method developed used a reversible phase column of C18, 3.9 x 150 mm, 4 mm, at 30 oC. The mobile phase was made up of a 0.25 mM sodium phosphate buffer and 0.025 mM EDTA, pH 6.00, methanol and acetonitrile (40:30:30), rate of 1.0 mL/minute and wavelength of 305 nm. The validated method showed to be accurate, precise, robust, linear and specific, in addition to being fast and practical, able to be used for analytic dosing of nystatin vaginal cream.","PeriodicalId":21193,"journal":{"name":"Revista Brasileira De Ciencias Farmaceuticas","volume":"07 1","pages":"637-643"},"PeriodicalIF":0.0,"publicationDate":"2008-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85978739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Aspectos atuais sobre aminocidos de cadeia ramificada e exerccio fsico

Revista Brasileira De Ciencias Farmaceuticas Pub Date : 2008-12-01 DOI: 10.1590/S1516-93322008000400004

Marcelo Macedo Rogero, Júlio Tirapegui

{"title":"Aspectos atuais sobre aminocidos de cadeia ramificada e exerccio fsico","authors":"Marcelo Macedo Rogero, Júlio Tirapegui","doi":"10.1590/S1516-93322008000400004","DOIUrl":"https://doi.org/10.1590/S1516-93322008000400004","url":null,"abstract":"ABSTRACT Current aspects of branched chainamino acid and exercise In healthy humans, nine amino acids are considered to beessential once they cannot be endogenously synthesisedand must therefore be ingested in the diet. Amongst theessential amino acids are the three branched chain aminoacids, namely, leucine, valine and isoleucine. These aminoacids participate in the regulation of protein balance inaddition to being nitrogen sources for the synthesis ofalanine and glutamine. As to the regulation of muscleprotein synthesis, leucine acts in the stimulation ofinitiation of mRNA translation into protein, both throughmechanisms that are dependent and independent ofinsulin. In the physiology of physical exercise, thesebranched amino acids play a role in central fatiguehypothesis, in muscle protein balance, in the secretion ofinsulin, in the modulation of the immune response, inperformance enhancement of individuals who work out inhot environments, and in avoiding muscle lesion. Thisreview approaches all aspects of the metabolism of andsupplementation with branched chain amino acids inphysical exercise.UNITERMS: Branched chain amino acids. Physicalexercise. Central fatigue. Immune response. Musclelesion. Protein synthesis.","PeriodicalId":21193,"journal":{"name":"Revista Brasileira De Ciencias Farmaceuticas","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2008-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83626699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 37

Influência do hábito de fumar na excreção urinária do ácido trans, trans-mucônico 吸烟习惯对尿中反式、反式粘液酸排泄的影响

Revista Brasileira De Ciencias Farmaceuticas Pub Date : 2008-09-01 DOI: 10.1590/S1516-93322008000300016

Maico de Menezes, Marina Salviato Balbão, M. E. Siqueira, I. Martins

{"title":"Influência do hábito de fumar na excreção urinária do ácido trans, trans-mucônico","authors":"Maico de Menezes, Marina Salviato Balbão, M. E. Siqueira, I. Martins","doi":"10.1590/S1516-93322008000300016","DOIUrl":"https://doi.org/10.1590/S1516-93322008000300016","url":null,"abstract":"Tobacco smoking is the major individual benzene source for no occupationally exposed subjects. This solvent is a volatile carcinogen and can induce serious health problems. Several biomarkers for benzene have been proposed, the most used is its metabolite trans,trans-muconic acid (ttMA) in urine, a suitable indicator since it reflects the internal dose of low benzene exposure. The present study aimed to evaluate the influence of smoking on urinary ttMA excretion. The analysis was performed by High Pressure Liquid Chromatography in a reversed-phase column and UV detection, after extraction of ttAM from urine using SAX cartridges. The method was validated, showing linearity between 0.006 to 10 µg mL-1 (r= 0.996), detection and quantification limits of 1.41 x 10-3 µg mL-1 and 6.0 x 10-3 µg mL-1 respectively, CVs from 1.79 to 2.91% (intra assay precision) and 3.53 to 18.37% (interassay precision). A significant positive correlation was observed between ttMA excretion and smoking (p= 0.005388). However, the cigarette number smoked by day seems have no influence on the urinary ttMA excretion.","PeriodicalId":21193,"journal":{"name":"Revista Brasileira De Ciencias Farmaceuticas","volume":"40 1","pages":"459-464"},"PeriodicalIF":0.0,"publicationDate":"2008-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75748974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Efeito de promotores na absorção percutânea in vitro do piroxicam a partir de formulações magistrais 促进剂对吡罗昔康体外经皮吸收的影响

Revista Brasileira De Ciencias Farmaceuticas Pub Date : 2008-09-01 DOI: 10.1590/S1516-93322008000300013

Francieli Furlan Bortolon, M. E. Sato, Regina Celis da Silveira Andreazza, Tania Mari Bellé Bresolin

{"title":"Efeito de promotores na absorção percutânea in vitro do piroxicam a partir de formulações magistrais","authors":"Francieli Furlan Bortolon, M. E. Sato, Regina Celis da Silveira Andreazza, Tania Mari Bellé Bresolin","doi":"10.1590/S1516-93322008000300013","DOIUrl":"https://doi.org/10.1590/S1516-93322008000300013","url":null,"abstract":"Formulacoes de piroxicam com base Lanette® (L) ou Net Fs ® (N), com ou sem promotores de absorcao (etanol-E ou propilenoglicol-P), foram desenvolvidas. O estudo de permeacao de piroxicam das formulacoes foi realizado em celula de Franz, usando pele de porco e o desempenho foi comparado com a especialidade farmaceutica. O farmaco permeado foi analisado por metodo desenvolvido por CLAE usando uma coluna analitica C18 de 15 x 0,46 cm, com fase movel metanol: tampao fosfato (60:40), comprimento de onda de 354 nm e o tempo de corrida foi de 10 min. O ensaio foi validado e apresentou limite de quantificacao de 0,138 mg/mL , linearidade na faixa de 0,02-5 µg/mL e nao ocorreu interferencia de substâncias endogenas da pele ou da formulacao. A ordem de permeacao de piroxicam, apos 24 horas, foi LE > L> Feldene® > N > LP > NP > NE. As formulacoes com L mostraram maior permeacao de piroxicam quando comparado com as formulacoes de base N. Entre os promotores, o etanol promoveu a maior permeacao do farmaco. Os resultados mostraram o desenvolvimento de formulacoes topicas economicas, de facil obtencao, com efetiva permeacao de piroxicam possibilitando a escolha de formulacoes com maior ou menor permeacao do farmaco.","PeriodicalId":21193,"journal":{"name":"Revista Brasileira De Ciencias Farmaceuticas","volume":"38 1","pages":"433-440"},"PeriodicalIF":0.0,"publicationDate":"2008-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76155362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5