Revista Brasileira De Ciencias Farmaceuticas最新文献_第10页

Determinação da composição físico-química de produtos lácteos: estudo exploratório de comparação dos resultados obtidos por metodologia oficial e por ultra-som 乳制品理化成分的测定:用官方方法和超声方法比较结果的探索性研究

Revista Brasileira De Ciencias Farmaceuticas Pub Date : 2007-12-01 DOI: 10.1590/S1516-93322007000400014

Raphael C. Venturoso, Keila Emílio de Almeida, Alexandre Mariani Rodrigues, M. R. Damin, Maricê Nogueira de Oliveira

{"title":"Determinação da composição físico-química de produtos lácteos: estudo exploratório de comparação dos resultados obtidos por metodologia oficial e por ultra-som","authors":"Raphael C. Venturoso, Keila Emílio de Almeida, Alexandre Mariani Rodrigues, M. R. Damin, Maricê Nogueira de Oliveira","doi":"10.1590/S1516-93322007000400014","DOIUrl":"https://doi.org/10.1590/S1516-93322007000400014","url":null,"abstract":"The present paper aims to compare the official methods of physical-chemical determination of dairy products (defatted dry matter, protein, fat and density) with methodology using ultra-sound. Different products were analyzed: milks (integral and skimmed conventional and organic), commercial fermented milks and dairy milk bases prepared in laboratory and whey totalizing twenty dairy products of different characteristics. The method using ultra-sound is fast and the results are correlated with those of the official analyses. However, the operator of the equipment should be attentive to employ a specific profile of the equipment according to the analysis to be carried out and, to calibrate the equipment based in the information obtained by the official analyses. The results of this exploratory article suggest the need to perform a study with a great number of samples in order to validate the obtained conclusions. The evidences described in this article are important for the Brazilian industry of dairy products.","PeriodicalId":21193,"journal":{"name":"Revista Brasileira De Ciencias Farmaceuticas","volume":"1 1","pages":"607-613"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73810967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 31

Validação de metodologia para dosagem de porfirinas urinárias por cromatografia líquida de alta eficiência 高效液相色谱法测定尿卟啉的方法验证

Revista Brasileira De Ciencias Farmaceuticas Pub Date : 2007-12-01 DOI: 10.1590/S1516-93322007000400011

Atecla Nunciata Lopes Alves, Marcelo N Burattini, N. Sumita, H. Rosa

{"title":"Validação de metodologia para dosagem de porfirinas urinárias por cromatografia líquida de alta eficiência","authors":"Atecla Nunciata Lopes Alves, Marcelo N Burattini, N. Sumita, H. Rosa","doi":"10.1590/S1516-93322007000400011","DOIUrl":"https://doi.org/10.1590/S1516-93322007000400011","url":null,"abstract":"Porphyrins are products that originate from the heme biosyntetic pathway. Enzymes that take part in this route can have their activity inhibited due to inherited/acquired or both factors resulting in increased serum heme precursor that will be eliminated in urine. Considering the importance of early detection of heme biosynthesis alterations, the purpose of this study was to establish a high pressure liquid chromatographic (HPLC) method with fluorescence detection, to detect five fractions of porphyrins: uroporphyrin (8-carboxyporphyrin), heptaporphyrin (7-carboxyporphyrin), hexaporphyrin (6-carboxyporphyrin), pentaporphyrin (5-carboxyporphyrin) and coproporphyrin I and III (4- carboxyporphyrin). Extraction methods with spectrophotometric detection are not sensitive enough for this purpose. The HPLC (Shimadzu Co., Kioto, Japan) was composed of two high-pressure pumps, auto-sampler and fluorescence detector (RF-535) with excitation at 400 nm and emission at 620 nm. The sample was eluted from a reversed-phase column with a linear gradient. The linearity of the method was from 8.0 to 120 µg/L for all fractions, proving its ablility to identify and quantify porphyrins with differents carboxylic groups for early diagnosis and follow-up of porphyrias.","PeriodicalId":21193,"journal":{"name":"Revista Brasileira De Ciencias Farmaceuticas","volume":"10 1","pages":"581-588"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87664034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Immunogenicity and allergenicity of 2S, 7S and 11S soy protein fractions 大豆蛋白2S、7S和11S组分的免疫原性和致敏性

Revista Brasileira De Ciencias Farmaceuticas Pub Date : 2007-12-01 DOI: 10.1590/S1516-93322007000400013

A. L. Bittencourt, M. F. Soares, R. Pirès, Cristina Sayuri Honmoto, M. Tanaka, C. Jacob, D. Abdalla

{"title":"Immunogenicity and allergenicity of 2S, 7S and 11S soy protein fractions","authors":"A. L. Bittencourt, M. F. Soares, R. Pirès, Cristina Sayuri Honmoto, M. Tanaka, C. Jacob, D. Abdalla","doi":"10.1590/S1516-93322007000400013","DOIUrl":"https://doi.org/10.1590/S1516-93322007000400013","url":null,"abstract":"It is known that in a part of the population, mainly among children, some are hypersensitive to soybean protein, although it is not yet completely elucidated which protein fraction is more immunogenic/allergenic. The objective of the study was to compare the immunogenicity and allergenicity of the soy protein fractions. The 2S (conglycinin), 7S (b conglycinin) and 11S (glycinin) fractions were isolated from soy protein by affinity chromatography. These purified soy protein fractions were used as antigens for immunizing BALB/c mice to evaluate their immunogenicity by following the appearance of specific IgM and IgG antibodies in blood serum by ELISA. The allergenicity of these soy protein fractions was evaluated by the following approaches: i) the production of IgE antibodies against 2S, 7S and 11S soy protein fractions by BALBc mice in the anaphylactic cutaneous passive test (PCA), and ii) the production of IgG1 specific antibodies against the 7S fraction in BALB/c mice. The 7S and 11S fractions induced the formation of IgM and IgG antibodies. The PCA test showed that only the 7S fraction was allergenic leading to the production of IgE antibodies. Another evidence that reinforces the allergenicity of the 7S soy protein fraction is the presence of IgG1 specific antibodies reactive to this protein fraction in immunized mice. Our study shows that the 7S soy protein fraction is important to elicit allergic reactions in mice and may contribute to elucidate the allergenicity of soy-derived products.","PeriodicalId":21193,"journal":{"name":"Revista Brasileira De Ciencias Farmaceuticas","volume":"29 1","pages":"597-606"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88095901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Comparative study on two rapid and sensitive methods for quantitative determination of tenoxicam in tablets 两种快速灵敏方法测定片中替诺昔康含量的比较研究

Revista Brasileira De Ciencias Farmaceuticas Pub Date : 2007-12-01 DOI: 10.1590/S1516-93322007000400015

Ashutosh Kumar Singh, P. García, F. Gomes, É. Kedor-Hackmann, M. Santoro

{"title":"Comparative study on two rapid and sensitive methods for quantitative determination of tenoxicam in tablets","authors":"Ashutosh Kumar Singh, P. García, F. Gomes, É. Kedor-Hackmann, M. Santoro","doi":"10.1590/S1516-93322007000400015","DOIUrl":"https://doi.org/10.1590/S1516-93322007000400015","url":null,"abstract":"Tenoxicam, a piroxicam analogue, is an NSAID (Non-Steroid Antinflamatory Drug). It is used in the symptomatic management of musculoskeletal and joint disorders such as osteoarthritis and rheumatoid arthritis, and also in the short-term management of soft-tissue injury. Its quantitative determination in pharmaceutical formulations is important to guarantee the desired therapeutic effects. The objective of this research was to develop, validate and compare spectrophotometric and chromatographic methods in the quantitative determination of tenoxicam in tablet preparations. In this work, tablets containing 20.0 mg of tenoxicam from different origins were analyzed. The spectrophotometric method was validated using 0.1 mol/L NaOH as solvent and a signal at 368 nm was taken. The HPLC method was validated using Synergi Hydro-RP® C18 column (250x4.6 mm, 4 µm). The mobile phase was constituted of methanol-water (61:39 v/v) with pH adjusted to 2.5 with formic acid, at a flow rate of 1.0 mL/min. UV detection was made at 375 nm. All analyses were performed with a column temperature of 25 °C ± 1. The calibration curves were linear over a concentration range from 4.0-24.0 µg/mL with a correlation coefficient better than 0.9999. The detection limit (DL) and quantitation limit (QL) were 0.25 µg/mL and 0.90 µg/mL for UV method and 0.35 µg/mL and 1.20 µg/mL for HPLC method respectively. The intra-day and inter-day precision expressed as RSD were below 2% for both methods. The mean recovery of tenoxicam was found to be in the range of 98.5-101.25% for UV method and 99.01-101.93% for HPLC method. The UV and HPLC methods were found to be rapid, precise and accurate. Statistically there was no significant difference between proposed UV spectrophotometric and HPLC methods.","PeriodicalId":21193,"journal":{"name":"Revista Brasileira De Ciencias Farmaceuticas","volume":"50 1","pages":"615-622"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86140399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Formas farmacêuticas sólidas orais de liberação prolongada: sistemas monolíticos e multiparticulados 口服固体缓释剂型:单片和多颗粒体系

Revista Brasileira De Ciencias Farmaceuticas Pub Date : 2007-12-01 DOI: 10.1590/S1516-93322007000400002

Bianca Ramos Pezzini, M. Silva, H. Ferraz

引用次数: 68

Cotton gauze bandage: a support for protease immobilization for use in biomedical applications 棉纱布绷带:用于生物医学应用的蛋白酶固定化的支持物

Revista Brasileira De Ciencias Farmaceuticas Pub Date : 2007-12-01 DOI: 10.1590/S1516-93322007000400006

I. J. Seabra, M. H. Gil

{"title":"Cotton gauze bandage: a support for protease immobilization for use in biomedical applications","authors":"I. J. Seabra, M. H. Gil","doi":"10.1590/S1516-93322007000400006","DOIUrl":"https://doi.org/10.1590/S1516-93322007000400006","url":null,"abstract":"Neste trabalho foi efetuada a imobilizacao de tripsina numa gaze esterilizada de algodao. Foram determinadas as condicoes otimas de imobilizacao: foi estudada a influencia do pH, concentracao e volume da solucao de tripsina usada na imobilizacao na hidrolise da N-benzoil-DL-arginina p-nitroanilida. As propriedades cataliticas, os parâmetros cineticos e as condicoes de estabilidade das enzimas livre e imobilizada foram comparadas. Os resultados mostraram que o pH e a temperatura otimos para a tripsina imobilizada foram 9.5 e 55 oC, respectivamente, maiores que os correspondentes da forma livre (7,5 e 45 oC). A 37 oC e a pH 7,0 (aproximadamente as condicoes fisiologicas) Km (constante de Michaelis) foi 3,98 µmol/mL e Vmax (velocidade maxima de reaccao) foi 0,719 µmol/(min mg) para a tripsina imobilizada; para a tripsina livre os valores correspondentes foram 2,46 µmol/mL e 2,89 µmol/(min mg). A quantidade de enzima imobilizada foi de 6 mg/g (base seca). Apos 30 dias nao se verificou libertacao de tripsina do suporte. O bom desempenho da tripsina imobilizada na gaze esterilizada de algodao comprova a sua potencial utilizacao como agente anti-inflamatorio no tratamento de lesoes cutâneas.","PeriodicalId":21193,"journal":{"name":"Revista Brasileira De Ciencias Farmaceuticas","volume":"22 1","pages":"535-542"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81918458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 28

Herbal products toxicology and clinical pharmacology 草药产品毒理学和临床药理学

Revista Brasileira De Ciencias Farmaceuticas Pub Date : 2007-12-01 DOI: 10.1590/S1516-93322007000400024

E. M. Bacchi

引用次数: 14

Caracterização físico-química de complexos de insulina: dimetil-beta-ciclodextrina e insulina: hidroxipropil-beta-ciclodextrina e avaliação da influência do tipo de complexo na produção de microesferas biodegradáveis 胰岛素配合物:二甲基-环糊精和胰岛素:羟丙基-环糊精的理化表征及配合物类型对生物可降解微球生产的影响评价

Revista Brasileira De Ciencias Farmaceuticas Pub Date : 2007-12-01 DOI: 10.1590/S1516-93322007000400007

V. C. Fernandes, Â. Denadai, R. D. S. Millán, Ricardo José Alves, Armando Da Silva Braga Junior

{"title":"Caracterização físico-química de complexos de insulina: dimetil-beta-ciclodextrina e insulina: hidroxipropil-beta-ciclodextrina e avaliação da influência do tipo de complexo na produção de microesferas biodegradáveis","authors":"V. C. Fernandes, Â. Denadai, R. D. S. Millán, Ricardo José Alves, Armando Da Silva Braga Junior","doi":"10.1590/S1516-93322007000400007","DOIUrl":"https://doi.org/10.1590/S1516-93322007000400007","url":null,"abstract":"The main stage in the linking and activation of the specific receptors by the insulin is the dissociation of this peptide hexamers, normally present in pharmaceutical formulations, in the monomeric active form. Because of this, the use of different cyclodextrins as adjuvants in the formulations containing insulin has been explored and the realized studies have demonstrated that the cyclodextrins can increase the absorption of the insulin mainly by reducing the ability of insulin oligomerization in aqueous media. In this work, complexes of INS:HP-b-CD and INS:DM-b-CD have been characterized by the use of isothermal calorimetry titration (ICT) and dynamic scattering of light. By means of ICT, the thermodynamic parameters of interaction between insulin and the cyclodextrins have been determined, and it was observed that the complexation occurs with an increase of entropy for both systems. The experiments of dynamic scattering of light have not showed reduction in the size of insulin particles, which could indicate the dissociation of insulin hexamers after the complexation with cyclodextrins. Then, the INS: HP-b-CD and INS:DM-b-CD complexes were encapsulated in PLGA microspheres. These systems were characterized and it was not observed any significant difference in the microspheres diameter, but a considerable increase in the hormone loading after the complexation with HP-b-CD and DM-b-CD was shown.","PeriodicalId":21193,"journal":{"name":"Revista Brasileira De Ciencias Farmaceuticas","volume":"46 1","pages":"543-553"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88631776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Target discovery and validation reviews and protocols 目标发现和验证评审和协议

Revista Brasileira De Ciencias Farmaceuticas Pub Date : 2007-12-01 DOI: 10.1590/S1516-93322007000400021

S. P. Farsky

引用次数: 2

Polyamine cell signaling 多胺细胞信号

Revista Brasileira De Ciencias Farmaceuticas Pub Date : 2007-12-01 DOI: 10.1590/S1516-93322007000400025

R. Hirata

引用次数: 4