Revista Brasileira De Ciencias Farmaceuticas最新文献_第2页

Síntese e atividade biológica do derivado 6-formil-oxamniquina

Revista Brasileira De Ciencias Farmaceuticas Pub Date : 2008-12-01 DOI: 10.1590/S1516-93322008000400022

Adélia Emília de Almeida, A. L. G. D. Souza, Silmara Marques Allegretti, Tarsila Ferraz Frezza, M. C. Pinto, Antonio G. Ferreira, Man Chin Chung

引用次数: 4

Propriedades antioxidantes em subproduto do pedúnculo de caju (Anacardium occidentale L.): efeito sobre a lipoperoxidação e o perfil de ácidos graxos poliinsaturados em ratos 腰果梗副产物的抗氧化性能:对大鼠脂质过氧化和多不饱和脂肪酸谱的影响

Revista Brasileira De Ciencias Farmaceuticas Pub Date : 2008-12-01 DOI: 10.1590/S1516-93322008000400025

P. R. B. Broinizi, Elma R. S. Andrade-Wartha, A. L. D. O. E. Silva, Rosângela Pavan Torres, H. M. Azeredo, R. Alves, J. Mancini-Filho

{"title":"Propriedades antioxidantes em subproduto do pedúnculo de caju (Anacardium occidentale L.): efeito sobre a lipoperoxidação e o perfil de ácidos graxos poliinsaturados em ratos","authors":"P. R. B. Broinizi, Elma R. S. Andrade-Wartha, A. L. D. O. E. Silva, Rosângela Pavan Torres, H. M. Azeredo, R. Alves, J. Mancini-Filho","doi":"10.1590/S1516-93322008000400025","DOIUrl":"https://doi.org/10.1590/S1516-93322008000400025","url":null,"abstract":"The cashew apple (Anacardium occidentale L.) contains phenolic compounds usually related with antioxidant properties. Then, the aim of this study was to investigate its antioxidant capacity. The antioxidant capacity of the hydroalcoholic extract of the cashew apple pulp (EHAlc.) was assessed for the scavenging of the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) by in vitro method and by an in vivo essay. For this essay a 30-day oral (gavage, EHAlc. 200 and 400 mg/kg) study was conducted in Wistar male rats, evaluating hepatic, plasma and brain tissues. In DPPH model, the extract demonstrated antioxidant activity of 95 % (largest concentration, 1000 µg/ mL). There were found no relevant peroxidation comparing the treated animals with the control group. However, the treated group presented a lower level of brain lipoperoxidation. Also in the treated animals brain tissue was found the largest amount of polyunsaturated fatty acids (PUFA), mainly docosahexaenoic (DHA). Therefore, the analyzed extract from cashew apple pulp clone CCP-76 contains effective natural antioxidants, responsible for free radical scavenging in vitro and also for decreasing the brain lipoperoxidation and keeping the PUFAS levels in Wistar rats.","PeriodicalId":21193,"journal":{"name":"Revista Brasileira De Ciencias Farmaceuticas","volume":"5 1","pages":"773-781"},"PeriodicalIF":0.0,"publicationDate":"2008-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78600753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Validação de método analítico espectrofotométrico UV para determinação de ácido úsnico em lipossomas 紫外分光光度法测定脂质体中松香酸的分析方法的验证

Revista Brasileira De Ciencias Farmaceuticas Pub Date : 2008-12-01 DOI: 10.1590/S1516-93322008000400008

Marigilson P. Siqueira-Moura, M. C. B. Lira, Nereide S. Santos-Magalhães

{"title":"Validação de método analítico espectrofotométrico UV para determinação de ácido úsnico em lipossomas","authors":"Marigilson P. Siqueira-Moura, M. C. B. Lira, Nereide S. Santos-Magalhães","doi":"10.1590/S1516-93322008000400008","DOIUrl":"https://doi.org/10.1590/S1516-93322008000400008","url":null,"abstract":"The secondary lichen metabolite usnic acid [2,6-diacetyl-7,9-dihydroxy-8,9b-dimethyl-1,3(2H,9bH)-dibenzofuran] has demonstrated pharmacological potential activities such as antitumor, antimicrobial, antiviral, antiproliferative, and anti-inflammatory. Liposomes are vesicles composed of phospholipid bilayers surrounding aqueous compartments and they have been used as colloidal drug carriers. The aim of this study was to develop and validate a quantitative UV spectrophotometric method for determination of usnic acid in liposomal formulations. The validation parameters were assessed according to The International Conference on Harmonization (ICH) and American Pharmacopoeia guidelines. The linearity range was of 3-15 µg.mL-1, regression equation: absorbance = 0.070 x UA concentration (µg.mL-1) + 0.013, and r = 0.9997. The repeatability (relative standard deviation) of the method was 1.96% and intermediate precision indicated that the difference among mean was statistically insignificant (P < 0.05). The accuracy revealed a mean percentage recovery of 100.4% of usnic acid. The method was robust for the variation of temperature and solvent. The detection and quantization limits were found to be 0.34 and 1.13 µg.mL-1, respectively. The content of usnic acid in liposomes was of 96.8% (± 0.2). The proposed method is accurate, precise and reproducible for estimation of usnic acid as raw material and in pharmaceutical dosage forms such as liposomes.","PeriodicalId":21193,"journal":{"name":"Revista Brasileira De Ciencias Farmaceuticas","volume":"117 1","pages":"621-628"},"PeriodicalIF":0.0,"publicationDate":"2008-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80384054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Hormônio do crescimento e exercício físico: considerações atuais 生长激素和体育锻炼:当前的考虑

Revista Brasileira De Ciencias Farmaceuticas Pub Date : 2008-12-01 DOI: 10.1590/S1516-93322008000400003

Vinicius Fernandes Cruzat, J. D. Júnior, Júlio Tirapegui, C. D. Schneider

{"title":"Hormônio do crescimento e exercício físico: considerações atuais","authors":"Vinicius Fernandes Cruzat, J. D. Júnior, Júlio Tirapegui, C. D. Schneider","doi":"10.1590/S1516-93322008000400003","DOIUrl":"https://doi.org/10.1590/S1516-93322008000400003","url":null,"abstract":"Although growth hormone (GH) is one of the most extensively studied hormones, various aspects related to this hormone have not been completely established, including its relationship with physical exercise. Recent studies have contributed to the understanding of the mechanisms of action of GH, which can be divided into 1) direct actions mediated by intracellular signals that are triggered by the binding of GH to its receptor on the plasma membrane, and 2) indirect actions mediated mainly by the regulation of the synthesis of insulin-like growth factors (IGF). Physical exercise has been shown to be a potent stimulator of GH release, especially in young men and women. The magnitude of this increase is influenced by several factors, especially the intensity and volume of exercise, in addition to training status. In this respect, athletes normally present a lower exercise-induced GH release than sedentary or poorly trained individuals. Experimental evidence indicates that GH may 1) favor the mobilization of free fatty acids from adipose tissue for energy generation, 2) increase the capacity of fat oxidation, and 3) increase energy expenditure.","PeriodicalId":21193,"journal":{"name":"Revista Brasileira De Ciencias Farmaceuticas","volume":"73 1","pages":"549-562"},"PeriodicalIF":0.0,"publicationDate":"2008-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75126552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Avaliação da atividade antiamnésica da casca de C. phlomidis Linn. em camundongos 金龟子树皮抗遗忘活性的评价。在小鼠体内

Revista Brasileira De Ciencias Farmaceuticas Pub Date : 2008-12-01 DOI: 10.1590/S1516-93322008000400019

anumanthachar Joshi, Krupa Megeri

{"title":"Avaliação da atividade antiamnésica da casca de C. phlomidis Linn. em camundongos","authors":"anumanthachar Joshi, Krupa Megeri","doi":"10.1590/S1516-93322008000400019","DOIUrl":"https://doi.org/10.1590/S1516-93322008000400019","url":null,"abstract":"Clerodendron phlomidis Linn. (Verbenaceae) e conhecida como Agnimantha em sânscrito. A casca da planta e utilizada no tratamento de varias disfuncoes neurologicas. No presente estudo, C. phlomidis foi investigada pelo seu potencial como agente nootropico em camundongos. O extrato aquoso de C. phlomidis (100 e 200 mg/kg, p.o.) foi administrado por seis dias consecutivos tanto para camundongos jovens quanto para idosos. Modelos comportamentais exteroceptivos, tais como labirinto em cruz elevada e paradigma de esquiva passiva foram empregados para avaliar memoria recente e tardia, respectivamente. Escopolamina (0,4 mg/kg i.p.), diazepam (1 mg/kg i.p.) foram empregados para induzir amnesia em camundongos. A fim de delinear o mecanismo pelo qual C. phlomidis exerce acao nootropica, determinaram-se seus efeitos nos niveis cerebrais de acetilcolinesterase. Utilizau-se piracetam (200 mg/kg i.p.) como nootropico padrao. O pre-tratamento com C. phlomidis (100 e 200 mg/kg, p.o.) por seis dias sucessivos melhorou, significativamente, o aprendizado e a memoria em camundongos. Ela reverteu a amnesia induzida por escopolamina, diazepam e pelo envelhecimento normal. Tambem, diminuiram-se os niveis de acetilcolinesteraseem todo o cerebro. A casca de C. phlomidis pode ser de grande uso no tratamento de disfuncoes cognitivas, como amnesia e doenca de Alzheimer.","PeriodicalId":21193,"journal":{"name":"Revista Brasileira De Ciencias Farmaceuticas","volume":"140 1","pages":"717-725"},"PeriodicalIF":0.0,"publicationDate":"2008-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88213859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Importância de polimorfismos de genes reguladores de citocinas em transplantes de células progenitoras hematopoiéticas 细胞因子调控基因多态性在造血祖细胞移植中的重要性

Revista Brasileira De Ciencias Farmaceuticas Pub Date : 2008-12-01 DOI: 10.1590/S1516-93322008000400021

Jeane Eliete Laguila Visentainer, A. M. Sell, D. A. S. Franceschi, S. R. Lieber, C. A. Souza

引用次数: 4

Excreção de gordura fecal de ratos (Rattus norvegicus, Wistar), submetidos a dietas hiperlipídicas e hipercolesterolêmicas suplementadas com quitosana 添加壳聚糖的高脂肪和高胆固醇饮食对褐家鼠粪便脂肪的排泄

Revista Brasileira De Ciencias Farmaceuticas Pub Date : 2008-12-01 DOI: 10.1590/S1516-93322008000400017

A.D.R. Cherem, Adriana Bramosrki

{"title":"Excreção de gordura fecal de ratos (Rattus norvegicus, Wistar), submetidos a dietas hiperlipídicas e hipercolesterolêmicas suplementadas com quitosana","authors":"A.D.R. Cherem, Adriana Bramosrki","doi":"10.1590/S1516-93322008000400017","DOIUrl":"https://doi.org/10.1590/S1516-93322008000400017","url":null,"abstract":"The effect of two comercial brands of chitosan in the faecal fat excretion was investigated in mice submitted to hypercholesterolemic and hyperlipidic diets. 32 rats Wistar, recently weaned, distributed randomly in four groups (n=8): control group (CG), received diet of AIN93G; hyperlipidic group (HG): diet of AIN-93G modified in the lipids content (12%) and supplemented with cholesterol (1%); and the chitosan hyperlipidic groups 1 (QHG1) and chitosan hyperlipidic 2 (QHG2), that received the same diet of the HG, supplemented with their respective chitosan (5%). The faecal collection was conducted on the days 0, 14th and 28th of the experiment. The faecal fat determination (g) was conducted through the Soxhlet method. No significant statistical differences (p>0.05) were observed among the 4 groups as to the fat content in the beginning of the experiment. On the 14th day, the GHQ1 group had a significant increase of faecal fat when compared to the other three groups (p<0.05). On the 28th day, in the GHQ1 group, the faecal fat excretion was larger than in the other groups (p<0.05), while the group GHQ2 had not statistically differed from the group GH. The results suggest that the differences between these two groups can be caused by the purity degree, viscosity and deacetylation degree of the chitosans.","PeriodicalId":21193,"journal":{"name":"Revista Brasileira De Ciencias Farmaceuticas","volume":"27 1","pages":"701-706"},"PeriodicalIF":0.0,"publicationDate":"2008-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75053705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Uma reviso sobre metalo--lactamases 金属内酰胺酶综述

Revista Brasileira De Ciencias Farmaceuticas Pub Date : 2008-12-01 DOI: 10.1590/S1516-93322008000400005

Claudia de Mello Bertoncheli, Rosmari Hörner

{"title":"Uma reviso sobre metalo--lactamases","authors":"Claudia de Mello Bertoncheli, Rosmari Hörner","doi":"10.1590/S1516-93322008000400005","DOIUrl":"https://doi.org/10.1590/S1516-93322008000400005","url":null,"abstract":"A emergencia e disseminacao da resistencia aos antimicrobianos sao problemas de grande importância mundial, particularmente entre patogenos nosocomiais de importância clinica como os bacilos Gram negativos nao fermentadores e membros da familia Enterobacteriaceae. O principal mecanismo de resistencia desses patogenos e a producao de β-lactamases, que sao enzimas que hidrolisam o anel β-lactâmico impedindo assim a acao dos antimicrobianos β-lactâmicos. As β-lactamases foram dividas em quatro classes de acordo com a sua estrutura primaria e podem tambem ser classificadas dentro de dois grupos com base no seu mecanismo catalitico, isto e, serina-β-lactamases (Classes A, C e D) e metalo-β-lactamases (Classe B). As metalo-β-lactamases (MβL) utilizam ions divalentes, comumente zinco, como co-fator para sua atividade catalitica e sao atualmente uma das classes que mais merece destaque, devido a sua capacidade de hidrolisar todos os antimicrobianos β-lactâmicos, incluindo os carbapenens. Estes antimicrobianos sao utilizados no tratamento de infeccoes causadas por bacterias Gram-negativas multirresistentes e conseguem se manter estaveis frente as serina-β-lactamases. A deteccao de microrganismos produtores de MβL tem por finalidade auxiliar a Comissao de Controle de Infeccao Hospitalar (CCIH) na prevencao da disseminacao desse mecanismo de resistencia no ambiente hospitalar e impedir que ele chegue ate a comunidade, bem como enfatizar o uso racional dos antimicrobianos disponiveis para uso clinico, pois, atualmente,ha poucos investimentos da industria farmaceutica na pesquisa de novos agentes antimicrobianos.","PeriodicalId":21193,"journal":{"name":"Revista Brasileira De Ciencias Farmaceuticas","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2008-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89424231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Validação de metodologia espectrofotométrica para quantificação dos flavonóides de Bauhinia cheilantha (Bongard) Steudel 紫荆花黄酮类化合物分光光度法定量方法的验证

Revista Brasileira De Ciencias Farmaceuticas Pub Date : 2008-12-01 DOI: 10.1590/S1516-93322008000400015

T. P. Sobrinho, C. E. D. Silva, J. Nascimento, Júlio Marcelino Monteiro, Ulysses Paulino de Albuquerque, E. L. C. D. Amorim

{"title":"Validação de metodologia espectrofotométrica para quantificação dos flavonóides de Bauhinia cheilantha (Bongard) Steudel","authors":"T. P. Sobrinho, C. E. D. Silva, J. Nascimento, Júlio Marcelino Monteiro, Ulysses Paulino de Albuquerque, E. L. C. D. Amorim","doi":"10.1590/S1516-93322008000400015","DOIUrl":"https://doi.org/10.1590/S1516-93322008000400015","url":null,"abstract":"Phytotherapeutic laboratories require access to methodologies that guarantee quality control for their products when these items are not already registered in pharmacopeias or official monographs. As such, the present study sought to validate analytical methodologies for quantifying natural flavonoids contained in extracts of \"pata-de-vaca\" (Bauhinia cheilantha [Bong.] Steudel) using visible light spectrophotometry and a rutin standard (µg/ml). All of the parameters required by ANVISA were evaluated. The specificity test revealed a maximum absorption peak at 420 nm. The methodology was considered linear and of high quantification sensitivity (2.51 µg/ml). The methodology also proved to be robust, and had high recovery levels (98.36%). The results obtained for repeatability, intermediate precision (intra- and inter-day), and reproducibility all certified the precision of the method, with values between 0.31 and 3.58%; the methodology was also considered exact (95.71-105.50%). This study demonstrated that the proposed methodology can be considered specific, precise, reproducible, exact, of low cost, and easy to perform.","PeriodicalId":21193,"journal":{"name":"Revista Brasileira De Ciencias Farmaceuticas","volume":"36 1","pages":"683-689"},"PeriodicalIF":0.0,"publicationDate":"2008-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90730391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 78

Atenção farmacêutica no contexto da estratégia de saúde da família 家庭保健战略范围内的药学服务

Revista Brasileira De Ciencias Farmaceuticas Pub Date : 2008-12-01 DOI: 10.1590/S1516-93322008000400020

A. Foppa, G. Bevilacqua, Luciano Henrique Pinto, C. Blatt

{"title":"Atenção farmacêutica no contexto da estratégia de saúde da família","authors":"A. Foppa, G. Bevilacqua, Luciano Henrique Pinto, C. Blatt","doi":"10.1590/S1516-93322008000400020","DOIUrl":"https://doi.org/10.1590/S1516-93322008000400020","url":null,"abstract":"A assistencia farmaceutica, no âmbito do Sistema Unico de Saude (SUS), e muitas vezes realizada com o objetivo primordial de garantir o abastecimento dos medicamentos nas Unidades Locais de Saude. Frequentemente, os cuidados com os pacientes e a promocao do uso racional dos medicamentos sao marginalizados. Contudo, durante o desenvolvimento do seguimento farmacoterapeutico, sao identificados problemas relacionados aos medicamentos (PRM) e dificuldades na adesao ao tratamento farmacologico, os quais estao relacionados a questoes familiares e sociais, e tem contribuido para o nao-sucesso terapeutico, portanto pontos-chave a serem considerados durante a Atencao Farmaceutica. Foi realizado o seguimento farmacoterapeutico de quatro pacientes atendidos em uma unidade de saude do municipio de Florianopolis. Para a resolucao dos PRMs, adotou-se abordagem que inclui os contextos familiar e social e, que utiliza ferramentas como o genograma e o ecomapa, alem do trabalho interdisciplinar. Observou-se a importância da familia e dos grupos de apoio para a resolucao dos PRMs e a necessidade do farmaceutico de aprimorar a pratica de atencao farmaceutica mediante a utilizacao de tais ferramentas. Percebeu-se, ainda, a importância das visitas domiciliares para conhecer melhor o ambiente familiar e a necessidade de trabalho interdisciplinar para melhorar a qualidade do atendimento.","PeriodicalId":21193,"journal":{"name":"Revista Brasileira De Ciencias Farmaceuticas","volume":"44 1","pages":"727-737"},"PeriodicalIF":0.0,"publicationDate":"2008-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77948103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17