Research最新文献_第9页

Delivery-Graded Programmable Micelles Achieve Enhanced Tumor Starvation through Combined Glutamine Deprivation and Angiogenesis Inhibition. 递送分级可编程胶束通过联合谷氨酰胺剥夺和血管生成抑制实现增强肿瘤饥饿。

IF 10.7 1区综合性期刊

Research Pub Date : 2025-09-05 eCollection Date: 2025-01-01 DOI: 10.34133/research.0858

Xuan Wei, Jiamin Cheng, Meijuan Geng, Siyu Chen, Liyang Gong, Siyu Meng, Keying Chen, Ziyan Wang, Zhang Yuan, Kaiyong Cai, Liangliang Dai

{"title":"Delivery-Graded Programmable Micelles Achieve Enhanced Tumor Starvation through Combined Glutamine Deprivation and Angiogenesis Inhibition.","authors":"Xuan Wei, Jiamin Cheng, Meijuan Geng, Siyu Chen, Liyang Gong, Siyu Meng, Keying Chen, Ziyan Wang, Zhang Yuan, Kaiyong Cai, Liangliang Dai","doi":"10.34133/research.0858","DOIUrl":"10.34133/research.0858","url":null,"abstract":"The inhibition of dependent glutamine metabolism is an effective treatment for triple-negative breast cancer (TNBC) starvation, but it is limited by compensatory glycolysis and inadequate delivery efficiency. Herein, we construct a pH-responsive size/charge-reprogrammed micelle with hierarchical delivery characteristics for TNBC suppression with glutamine depletion and vessel blockade. It consists of a positively charged prodrug micelle chemically grafted with the glutamine transport inhibitor V9302 as the inner core layer, the neovascular disruptor CA4P adsorbed in the middle layer, and a pH-responsive peelable polymer as the outer shell. The nanosystem PPD/PPQV@C could effectively reduce size and reverse charge in response to the tumor acidic microenvironment by removing the outer polymer PPD, as accompanying the release of CA4P. Furthermore, the remaining PPQV could responsively release V9302 in the cytoplasm of tumor cells, improving the bioavailability of cargoes and overcoming permeability barrier through precise hierarchical release strategy. Importantly, V9302 and CA4P localized in the tumor intracellular and extracellular matrix could effectively block TNBC-dependent glutamine metabolism and inhibit compensatory nutrient by blocking angiogenesis, achieving the desired tumor suppression with prolonged survival time. This work exhibits a smart nanoplatform for efficient TNBC treatment via dual blockade of the dependent glutamine metabolism and angiogenesis.","PeriodicalId":21120,"journal":{"name":"Research","volume":"5 ","pages":"0858"},"PeriodicalIF":10.7,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12411305/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145016177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Focusing on Microenvironmental Disturbance to Potentiate Reparative Ability of Neural Stem Cell After Stroke. 微环境干扰增强脑卒中后神经干细胞修复能力的研究。

IF 10.7 1区综合性期刊

Research Pub Date : 2025-09-05 eCollection Date: 2025-01-01 DOI: 10.34133/research.0829

Hongfei Ge, Haomiao Wang, Rong Hu

引用次数: 0

Direct Identification of O-Glycopeptides by Low-Temperature Assisted Nanopore Technique. 低温辅助纳米孔技术直接鉴定o型糖肽。

IF 10.7 1区综合性期刊

Research Pub Date : 2025-09-04 eCollection Date: 2025-01-01 DOI: 10.34133/research.0850

Jia-Hong Wang, Wenjing Ma, Zheng-Li Hu, Zhaobing Gao, Yi-Tao Long, Tiehai Li, Yi-Lun Ying

引用次数: 0

E-SegNet: E-Shaped Structure Networks for Accurate 2D and 3D Medical Image Segmentation. E-SegNet：用于精确分割二维和三维医学图像的e形结构网络。

IF 10.7 1区综合性期刊

Research Pub Date : 2025-09-03 eCollection Date: 2025-01-01 DOI: 10.34133/research.0869

Wei Wu, Xin Yang, Chenggui Yao, Ou Liu, Qi Zhao, Jianwei Shuai

{"title":"E-SegNet: E-Shaped Structure Networks for Accurate 2D and 3D Medical Image Segmentation.","authors":"Wei Wu, Xin Yang, Chenggui Yao, Ou Liu, Qi Zhao, Jianwei Shuai","doi":"10.34133/research.0869","DOIUrl":"10.34133/research.0869","url":null,"abstract":"U-structure has become a foundational approach in medical image segmentation, consistently demonstrating strong performance across various segmentation tasks. Most current models are based on this framework, customizing encoder-decoder components to achieve higher accuracy across various segmentation challenges. However, this often comes at the cost of increased parameter counts, which inevitably limit their practicality in real-world applications. In this study, we provide an E-shaped segmentation framework that discards the traditional step-by-step resolution recovery decoding process, instead directly aggregating multi-scale features extracted by the encoder at each stage for deep cross-level integration. Additionally, we propose an innovative multi-scale large-kernel convolution (MLKConv) module, designed to enhance high-level feature representation by effectively capturing both local and global contextual information. Compared to U-structure, the proposed E-structured approach substantially reduces parameters while delivering superior performance, especially in complex segmentation tasks. Based on this structure, we develop 2 segmentation networks specifically for 2-dimensional (2D) and 3D medical images. 2D E-SegNet is evaluated on four 2D segmentation benchmark datasets (Synapse multi-organ, ACDC, Kvasir-Seg, and BUSI), while 3D E-SegNet is assessed on four 3D segmentation benchmark datasets (Synapse, ACDC, NIH Pancreas, and Lung). Experimental results demonstrate that our approach outperforms the current leading U-shaped models across multiple datasets, achieving new state-of-the-art (SOTA) performance with fewer parameters. In summary, our research introduces a novel approach to medical image segmentation, offering potential improvements and contributing to ongoing advancements in the field. Our code is publicly available on https://github.com/zhaoqi106/E-SegNet.","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0869"},"PeriodicalIF":10.7,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12408157/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145001407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteogenomic Analysis Identifies Clinically Relevant Subgroups of Collecting Duct Carcinoma. 蛋白质基因组学分析鉴定收集管癌临床相关亚群。

IF 10.7 1区综合性期刊

Research Pub Date : 2025-09-03 eCollection Date: 2025-01-01 DOI: 10.34133/research.0859

Yuanyuan Qu, Xiaoru Pei, Jinwen Feng, Xin Yan, Linhui Zhang, Jun Wang, Xin Yao, Jiasheng Bian, Yu Gan, Hualei Gan, Xuewen Jiang, Ping Yang, Maoping Cai, Liqing Li, Xinqiang Wu, Weiwei Jing, Chao Zhang, Jianyuan Zhao, Hailiang Zhang, Guohai Shi, Xiang Zhou, Dingwei Ye, Chen Ding

{"title":"Proteogenomic Analysis Identifies Clinically Relevant Subgroups of Collecting Duct Carcinoma.","authors":"Yuanyuan Qu, Xiaoru Pei, Jinwen Feng, Xin Yan, Linhui Zhang, Jun Wang, Xin Yao, Jiasheng Bian, Yu Gan, Hualei Gan, Xuewen Jiang, Ping Yang, Maoping Cai, Liqing Li, Xinqiang Wu, Weiwei Jing, Chao Zhang, Jianyuan Zhao, Hailiang Zhang, Guohai Shi, Xiang Zhou, Dingwei Ye, Chen Ding","doi":"10.34133/research.0859","DOIUrl":"10.34133/research.0859","url":null,"abstract":"Collecting duct carcinoma (CDC) is a rare but aggressive form of renal cell carcinoma (RCC) that has limited understanding and an undefined systemic therapeutic regimen. Herein, we conducted a comprehensive proteogenomic analysis of CDC tumors and normal adjacent tissues to elucidate the biology of the disease. CDC exhibited high heterogeneity in tumor mutational burden, and enhanced ribosome biogenesis was the most striking malignant feature of CDC, even compared with other common kidney carcinomas. Genomic data indicated that UTP6 and HN1 amplification on chromosome 17q were associated with the activations of ribosome biogenesis and cell migration, respectively, which were relevant to tumor proliferation and metastasis. Proteomic-based classification identified 3 clusters, among which, tumors overexpressing ribosome biogenesis signaling (GP1) clustered into the most aggressive subtype, while tumors with increased energy metabolism (GP3) exhibited significant sensitivity to anti-vascular endothelial growth factor agents. Immune subtyping revealed a complex immune landscape of CDC. Additionally, increased RPF2, contributing to ribosome production, was validated to be associated with malignant phenotypes, and targeting RPF2 could exert an anti-oncogenic role by disrupting ribosome biogenesis and perturbing the MDM2-p53 interaction.","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0859"},"PeriodicalIF":10.7,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12408255/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145016139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ligustilide Suppresses Macrophage-Mediated Intestinal Inflammation and Restores Gut Barrier via EGR1-ADAM17-TNF-α Pathway in Colitis Mice. ligusliide通过EGR1-ADAM17-TNF-α途径抑制结肠炎小鼠巨噬细胞介导的肠道炎症并恢复肠道屏障

IF 10.7 1区综合性期刊

Research Pub Date : 2025-09-02 eCollection Date: 2025-01-01 DOI: 10.34133/research.0864

Yanyang Li, Yequn Wu, Jing Liang, Peiqi Chen, Shihua Xu, Yumei Wang, Zhi Jiang, Xudong Zhu, Chaozhan Lin, Yang Yu, Hailin Tang

{"title":"Ligustilide Suppresses Macrophage-Mediated Intestinal Inflammation and Restores Gut Barrier via EGR1-ADAM17-TNF-α Pathway in Colitis Mice.","authors":"Yanyang Li, Yequn Wu, Jing Liang, Peiqi Chen, Shihua Xu, Yumei Wang, Zhi Jiang, Xudong Zhu, Chaozhan Lin, Yang Yu, Hailin Tang","doi":"10.34133/research.0864","DOIUrl":"10.34133/research.0864","url":null,"abstract":"Ulcerative colitis is a chronic nonspecific intestinal inflammatory disease, which usually occurs in the rectal and colonic mucosa and submucosa. Ligustilide, a major component derived from Angelica sinensis (Oliv.) Diels, exerts anti-inflammation effect. However, its impact and molecular mechanism on colitis remain obscure. In this study, in vivo and in vitro experiments verified that ligustilide protected against colitis by suppressing macrophage-mediated inflammation and repairing intestinal barrier. Of note, we utilized a thermal proteome profiling strategy to preliminarily find early growth response factor 1 (EGR1) as a target of ligustilide. Cellular thermal shift assay, drug affinity responsive target stability, and surface plasmon resonance analysis revealed that ligustilide directly targeted His386 to bind to EGR1. Furthermore, RNA-sequencing, dual luciferase reporter gene assay, and rescue experiments illustrated that ligustilide disturbed the nuclear translocation of EGR1 and broke its combination with a disintegrin and metalloproteinase 17 (ADAM17) promoter, thereby inhibiting ADAM17 transcription and downstream tumor necrosis factor-α (TNF-α) production, as well as expression of inflammatory proteins cyclooxygenase 2 and inducible nitric oxide synthase. Finally, the in vivo experiment with EGR1 overexpression proved that EGR1 was essential for the protective effects of ligustilide on colitis mice. Taken together, our study demonstrates that ligustilide targets EGR1 to inhibit the EGR1-ADAM17-TNFα pathway, thus alleviating macrophage-mediated intestinal inflammation and restoring gut barrier.","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0864"},"PeriodicalIF":10.7,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12404647/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144993399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D Genome Engineering: Current Advances and Therapeutic Opportunities in Human Diseases. 3D基因组工程：人类疾病的最新进展和治疗机会。

IF 10.7 1区综合性期刊

Research Pub Date : 2025-09-01 eCollection Date: 2025-01-01 DOI: 10.34133/research.0865

Xing Jiang, Xiaoli Wang, Song Shen, Shangguo Hou, Chen Yu

引用次数: 0

Recent Advances in Metasurfaces: From THz Biosensing to Microwave Wireless Communications. 超表面的最新进展：从太赫兹生物传感到微波无线通信。

IF 10.7 1区综合性期刊

Research Pub Date : 2025-08-29 eCollection Date: 2025-01-01 DOI: 10.34133/research.0820

Yue Wang, Xiang Zhang, Yuxiang Wang, Yunfei Liu, Jiaxue Li, Xiangdong Chen, Zijian Cui, Shah Nawaz Burokur, Jingdi Zhang, Xiaoguang Zhao, Kuang Zhang, Zheng You

{"title":"Recent Advances in Metasurfaces: From THz Biosensing to Microwave Wireless Communications.","authors":"Yue Wang, Xiang Zhang, Yuxiang Wang, Yunfei Liu, Jiaxue Li, Xiangdong Chen, Zijian Cui, Shah Nawaz Burokur, Jingdi Zhang, Xiaoguang Zhao, Kuang Zhang, Zheng You","doi":"10.34133/research.0820","DOIUrl":"10.34133/research.0820","url":null,"abstract":"In recent years, important progress has been made in the field of biosensing and wireless communications by using metamaterials and metasurfaces. These technologies enable efficient manipulation of electromagnetic waves through judiciously designed subwavelength structural units. This review begins by focusing on the design and optimization of terahertz metasurface sensors, emphasizing their unique advantages in biomedical diagnostics. It explores key technical challenges, such as material selection, device integration, and development of robust sensor for surface-specific modifications. Furthermore, the review discusses how metasurfaces, particularly as reconfigurable intelligent surfaces, dynamically modulate electromagnetic wave propagation in the microwave communications domain to enhance signal quality, improve communication efficiency, and showcase their potential in 5G and future 6G technologies. Finally, a comprehensive overview is provided regarding the challenges and future research directions for metamaterial and metasurface technologies in both biosensing and wireless communications, with the ultimate goal of promoting their applications in point-of-care devices and efficient communication systems.","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0820"},"PeriodicalIF":10.7,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12395562/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GCDH Acetylation Orchestrates DNA Damage Response and Autophagy via Mitochondrial ROS to Suppress Hepatocellular Carcinoma Progression. GCDH乙酰化通过线粒体ROS调控DNA损伤反应和自噬抑制肝癌进展。

IF 10.7 1区综合性期刊

Research Pub Date : 2025-08-29 eCollection Date: 2025-01-01 DOI: 10.34133/research.0862

Wei Tian, Yue Yang, Lili Meng, Chao Ge, Yuqi Liu, Canxue Zhang, Zhihong Huang, Chi Zhang, Hua Tian

{"title":"GCDH Acetylation Orchestrates DNA Damage Response and Autophagy via Mitochondrial ROS to Suppress Hepatocellular Carcinoma Progression.","authors":"Wei Tian, Yue Yang, Lili Meng, Chao Ge, Yuqi Liu, Canxue Zhang, Zhihong Huang, Chi Zhang, Hua Tian","doi":"10.34133/research.0862","DOIUrl":"10.34133/research.0862","url":null,"abstract":"Metabolic enzyme dysregulation promotes hepatocellular carcinoma (HCC) progression through metabolic reprogramming and lysine acetylation. Glutaryl-CoA dehydrogenase (GCDH), a key enzyme in lysine metabolism, has been demonstrated to play an essential role in modulating lysine crotonylation, which impacts the progression of HCC. However, the specific mechanisms by which GCDH influences lysine acetylation in HCC have not been completely clarified. In this study, GCDH was found to be acetylated at lysine 438 by acetyltransferase P300 and deacetylated by HDAC1. GCDH K438 acetylation was critical for its tumor-suppressive function in HCC cells. Overexpression of GCDH led to elevated levels of reactive oxygen species (ROS) and reduced oxidative phosphorylation (OXPHOS), thereby triggering ATR/Chk1-mediated DNA damage repair dysfunction and promoting autophagy in HCC cells. Furthermore, our investigation demonstrated that decreased GCDH expression was markedly associated with shorter overall survival in HCC patients and served as an independent prognostic indicator. Collectively, our findings demonstrate that the acetylation of GCDH at lysine 438 (K438), mediated by P300 and HDAC1, plays a vital role in the tumor-suppressive activities of HCC cells. GCDH inhibits HCC progression through ROS-mediated DNA repair dysfunction and autophagy.","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0862"},"PeriodicalIF":10.7,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12395558/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ionic Transport Aspects of Water Electrolysis in Alkaline Media. 碱性介质中电解水的离子传输。

IF 10.7 1区综合性期刊

Research Pub Date : 2025-08-28 eCollection Date: 2025-01-01 DOI: 10.34133/research.0788

F ElBachraoui, D Aymé-Perrot, H H Girault

{"title":"Ionic Transport Aspects of Water Electrolysis in Alkaline Media.","authors":"F ElBachraoui, D Aymé-Perrot, H H Girault","doi":"10.34133/research.0788","DOIUrl":"10.34133/research.0788","url":null,"abstract":"Water electrolysis is a key industrial process for producing green hydrogen. To avoid the use of noble metals and fluorinated polymer membranes, liquid water electrolysis is often carried out in alkaline conditions. It is common to distinguish between 3 processes: alkaline electrolysis at high electrolyte concentrations (≥7 M) with porous membranes, alkaline electrolysis at high electrolyte concentrations (≥7 M) with ion-solvating membranes, and alkaline electrolysis at moderate electrolyte concentrations (<2 M) with anion-exchange membranes. Here, we consider the fundamental aspects of water and ion fluxes and of conductivity across the 3 types of membranes. We discuss ionic currents governed by ion-ion interactions and those resulting from a Grotthuss mechanism. Furthermore, in the case of porous membranes made of a polymeric fabric with compressed inorganic fillers such as zirconia, which are negatively charged in the presence of KOH, and of ion-solvating membranes such as polybenzimidazole, which also become negatively charged by deprotonation at high pH, those membranes should be a weak cation exchanger. We here address this dichotomy. All in all, we show that in all 3 cases, the membrane is an anion exchanger where hydroxide ion mobility differs from that of adjacent aqueous solution due to confinement favoring a Grotthuss-type transport and a jump mechanism.","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0788"},"PeriodicalIF":10.7,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12393796/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0