{"title":"Printability in Multi-material Projection-Based 3-Dimensional Bioprinting.","authors":"Chao-Fan He, Tian-Hong Qiao, Xu-Chao Ren, Mingjun Xie, Qing Gao, Chao-Qi Xie, Peng Wang, Yuan Sun, Huayong Yang, Yong He","doi":"10.34133/research.0613","DOIUrl":"10.34133/research.0613","url":null,"abstract":"<p><p>Accurately reconstructing the intricate structure of natural organisms is the long-standing goal of 3-dimensional (3D) bioprinting. Projection-based 3D printing boasts the highest resolution-to-manufacturing time ratio among all 3D-printing technologies, rendering it a highly promising technique in this field. However, achieving standardized, high-fidelity, and high-resolution printing of composite structures using bioinks with diverse mechanical properties remains a marked challenge. The root of this challenge lies in the long-standing neglect of multi-material printability research. Multi-material printing is far from a simple physical assembly of different materials; rather, effective control of material interfaces is a crucial factor that governs print quality. The current research gap in this area substantively hinders the widespread application and rapid development of multi-material projection-based 3D bioprinting. To bridge this critical gap, we developed a multi-material projection-based 3D bioprinter capable of simultaneous printing with 6 materials. Building upon this, we established a fundamental framework for multi-material printability research, encompassing its core logic and essential process specifications. Furthermore, we clarified several critical issues, including the cross-linking behavior of multicomponent bioinks, mechanical mismatch and interface strength in soft-hard composite structures, the penetration behavior of viscous bioinks within hydrogel polymer networks, liquid entrapment and adsorption phenomena in porous heterogeneous structures, and error source analysis along with resolution evaluation in multi-material printing. This study offers a solid theoretical foundation and guidance for the quantitative assessment of multi-material projection-based 3D bioprinting, holding promise to advance the field toward higher precision and the reconstruction of more intricate biological structures.</p>","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0613"},"PeriodicalIF":11.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11876545/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143557710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ResearchPub Date : 2025-02-28eCollection Date: 2025-01-01DOI: 10.34133/research.0614
Chenxin Lu, Chunjian Li, Ning Gu, Fang Yang
{"title":"Emerging Elastic Micro-Nano Materials for Diagnosis and Treatment of Thrombosis.","authors":"Chenxin Lu, Chunjian Li, Ning Gu, Fang Yang","doi":"10.34133/research.0614","DOIUrl":"https://doi.org/10.34133/research.0614","url":null,"abstract":"<p><p>Thrombus is a blood clot that forms in a blood vessel at the point of flaking. Thrombosis is closely associated with cardiovascular diseases caused by different sources and factors. However, the current clinical methods of thrombus diagnosis and treatment still have problems with targeting, permeability, stability, and biosafety. Therefore, in recent years, based on the development of micro/nano technology, researchers have tried to develop some new strategies for the diagnosis and treatment of thrombosis. Due to the unique structural characteristics, the micro-nano materials in physiological environments show excellent transport and delivery properties such as better in vivo circulation, longer life span, better targeting ability, and controllable cellular internalization. Especially, elasticity and stiffness are inherent mechanical properties of some well-designed micro-nano materials, which can make them better adapted to the needs of thrombosis diagnosis and treatment. Herein, this review first introduces the thrombotic microenvironment to characterize the thrombus development process. Then, to fine-tune the pathological occurrence and development of thrombosis, the role of elastic micro-nano materials for thrombus diagnosis and treatment is summarized. The properties, preparation methods, and biological fate of these materials have been discussed in detail. Following, the applications of elastic micro-nano materials in biomedical imaging, drug delivery, and therapy of thrombosis are highlighted. Last, the shortcomings and future design strategies of elastic micro-nano materials in diagnosis and treatment of clinical thrombosis are discussed. This review will provide new ideas for the use of nanotechnology in clinical diagnosis and treatment of thrombus in the future.</p>","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0614"},"PeriodicalIF":11.0,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11868703/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143543257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Millennium-Scale Atlantic Multidecadal Oscillation and Soil Moisture Influence on Western Mediterranean Cloudiness.","authors":"Nazzareno Diodato, Kristina Seftigen, Gianni Bellocchi","doi":"10.34133/research.0606","DOIUrl":"10.34133/research.0606","url":null,"abstract":"<p><p>Understanding long-term historical changes in cloudiness is essential for elucidating Earth's climate dynamics and variability and its extremes. In this study, we present the first millennial-length reconstruction of the annual total cloud cover (TCC) in the western Mediterranean, covering the period from 969 to 2022 CE. Based on a comprehensive set of hydrological and atmospheric variables, our reconstruction reveals a nuanced pattern of cloudiness evolution over the past millennium. We observe an initial increase in cloudiness until 1600 CE, followed by a substantial decrease in TCC. This shift was driven by a confluence of factors, including the eruption of Mount Tambora in Indonesia in 1815, increased solar forcing, and a positive phase of the Atlantic Multidecadal Oscillation. These complex dynamics have brought modern warming cloud patterns closer to those observed during the medieval period before c. 1250, exceeding the background variability of the Little Ice Age (c. 1250 to 1849). In particular, recent decades have witnessed an unprecedented coupling of intense solar activity, high temperatures, and the lowest cloud cover on record. Our results highlight the importance of inter-oceanic-scale relationships between Atlantic forcing mechanisms and the TCC in shaping future trends in western Mediterranean cloudiness. This study provides valuable insights into the long-term dynamics of cloudiness and its implications for regional climate trends in the western Mediterranean and beyond.</p>","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0606"},"PeriodicalIF":11.0,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11862910/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Gecko-Inspired Intelligent Adhesive Structures for Rough Surfaces.","authors":"Yawen Shao, Miao Li, Hongmiao Tian, Fabo Zhao, Jian Xu, Hongrong Hou, Zhijun Zhang, Duorui Wang, Xiaoliang Chen, Wenjun Li, Hongjian Yan, Jinyou Shao","doi":"10.34133/research.0630","DOIUrl":"10.34133/research.0630","url":null,"abstract":"<p><p>Biomimetic dry adhesive structures, inspired by geckos' climbing abilities, have attracted research attention in recent years. However, achieving superior adhesion on a rough surface remains an important challenge, which limits practical applications. Conventional bionic adhesion methods perform well on smooth surfaces, but adhesion strength drastically decreases on rough surfaces due to the reduced contact area. Generally, various adhesive structures have been proposed to increase the contact area without assessing adhesion states, against obtaining good performance on rough surfaces. If an intelligent adhesive approach could be introduced on rough surfaces, it would be beneficial for promoting the development of gecko-inspired adhesives. However, existing adhesive structures with the sensing function usually utilize the adhesive function to support the sensing function, i.e., a sensor with an adhesive function; for other few structures, the sensing function supports adhesion, but they do not focus on improving adhesion performance on rough surfaces. Inspired by the synergistic effect of a kinematic system during the crawling process of geckos, this study proposes an intelligent adhesive structure for rough surfaces. The proposed structure combines a hierarchical bionic dry adhesive structure based on gecko paw microhairs with a flexible capacitive sensor unit. Experimental observations and analytical modeling demonstrate that incorporating mushroom-shaped bionic dry adhesive structures with inclined support micropillars can reduce interface contact stiffness, notably enhancing adhesion on rough surfaces while allowing real-time monitoring of contact states. Moreover, this innovative smart adhesive structure facilitates morphology sensing of contact interfaces, presenting potential advancements in bionic adhesion for morphology sensing applications.</p>","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0630"},"PeriodicalIF":11.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11850978/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143503644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Circ-0001283 Aggravates Cardiac Hypertrophy by Targeting Myosin Light Chain 3 Protein.","authors":"Wenjing Wang, Lili Chen, Yiheng Zhao, Shuchen Zhang, Xiang Zhou","doi":"10.34133/research.0626","DOIUrl":"10.34133/research.0626","url":null,"abstract":"<p><p>Circular RNAs (circRNAs) are differentially expressed in cardiac hypertrophy; however, the exact function and mechanisms during hypertrophy development are still unknown. Here, we explored the role of a newly discovered circRNA in the pathogenesis of myocardial hypertrophy. It was found that circ-0001283 promoted the progression of cardiac hypertrophy by interacting with myosin light chain 3 (MYL3) to inhibit the protein ubiquitination and enhance its protein expression, not by the competitive endogenous RNA mechanism. Further investigation demonstrated that the reduced hypertrophy induced by circ-0001283 knockdown was counteracted by overexpression of MYL3. Mechanistically, MYL3 facilitated myocardial hypertrophy by inducing autophagy in cells via PI3K/Akt/mTOR and ERK signaling pathways. In summary, circ-0001283 can bind directly to MYL3 and up-regulate its expression, thereby promoting autophagy to accelerate cardiac hypertrophy. Circ-0001283 may serve as a potential therapeutic target for cardiac hypertrophy.</p>","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0626"},"PeriodicalIF":11.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11850654/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143503641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ResearchPub Date : 2025-02-24eCollection Date: 2025-01-01DOI: 10.34133/research.0627
Jian He, Jiawei Li, Yihan Sun, Yuanyuan Shen, Qi Wei, Dun Zhang, Danqing Feng, Peng Wang
{"title":"Molecular Mechanism of Oil-Infused Silicone Preventing Mussel Biofouling.","authors":"Jian He, Jiawei Li, Yihan Sun, Yuanyuan Shen, Qi Wei, Dun Zhang, Danqing Feng, Peng Wang","doi":"10.34133/research.0627","DOIUrl":"10.34133/research.0627","url":null,"abstract":"<p><p>Marine biofouling causes severe economical and environmental challenges to marine industries and maritime activities. Biofouling prevention has emerged as one of the most pressing issues in water-related industries. Recently, the slippery liquid-infused porous surfaces (SLIPSs) have shown great potential for biofouling prevention across a broad spectrum of fouling organisms. However, our understanding of the mechanisms by which SLIPSs prevent biofouling remains limited. In this study, we discovered that oil-infused polydimethylsiloxane elastomer (i-PDMS), a silicone-based SLIPS variant, significantly inhibited the sensory responses of the fouling mussel <i>Mytilopsis sallei</i>, particularly at its sensory organ, the foot. Using bioinformatics and molecular biology analyses, we demonstrated that i-PDMS disrupts larval settlement of <i>M. sallei</i> by interfering with the mechanosensitive transient receptor potential melastatin-subfamily member 7 (TRPM7) channel, which is highly expressed in the foot during the settlement process. Furthermore, adhesion assays and molecular dynamics simulations revealed that the secreted foot proteins of the mussel are unable to effectively interact with the i-PDMS surface due to nanoscale fluctuations at the material interface. These findings enhance our understanding of how fouling organisms sense and adhere to surfaces and provide deeper insights into the antifouling mechanisms of SLIPS.</p>","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0627"},"PeriodicalIF":11.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11848653/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143493450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ResearchPub Date : 2025-02-24eCollection Date: 2025-01-01DOI: 10.34133/research.0624
Anna Jancik-Prochazkova, Katsuhiko Ariga
{"title":"Nano-/Microrobots for Environmental Remediation in the Eyes of Nanoarchitectonics: Toward Engineering on a Single-Atomic Scale.","authors":"Anna Jancik-Prochazkova, Katsuhiko Ariga","doi":"10.34133/research.0624","DOIUrl":"10.34133/research.0624","url":null,"abstract":"<p><p>Nano-/microrobots have been demonstrated as an efficient solution for environmental remediation. Their strength lies in their propulsion abilities that allow active \"on-the-fly\" operation, such as pollutant detection, capture, transport, degradation, and disruption. Another advantage is their versatility, which allows the engineering of highly functional solutions for a specific application. However, the latter advantage can bring complexity to applications; versatility in dimensionality, morphology, materials, surface decorations, and other modifications has a crucial effect on the resulting propulsion abilities, compatibility with the environment, and overall functionality. Synergy between morphology, materials, and surface decorations and its projection to the overall functionality is the object of nanoarchitectonics. Here, we scrutinize the engineering of nano-/microrobots with the eyes of nanoarchitectonics: we list general concepts that help to assess the synergy and limitations of individual procedures in the fabrication processes and their projection to the operation at the macroscale. The nanoarchitectonics of nano-/microrobots is approached from microscopic level, focusing on the dimensionality and morphology, through the nanoscopic level, evaluating the influence of the decoration with nanoparticles and quantum dots, and moving to the decorations on molecular and single-atomic level to allow very fine tuning of the resulting functionality. The presented review aims to lay general concepts and provide an overview of the engineering of functional advanced nano-/microrobot for environmental remediation procedures and beyond.</p>","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0624"},"PeriodicalIF":11.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11848434/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143493452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ResearchPub Date : 2025-02-24eCollection Date: 2025-01-01DOI: 10.34133/research.0615
Minjie Mou, Zhichao Zhang, Ziqi Pan, Feng Zhu
{"title":"Deep Learning for Predicting Biomolecular Binding Sites of Proteins.","authors":"Minjie Mou, Zhichao Zhang, Ziqi Pan, Feng Zhu","doi":"10.34133/research.0615","DOIUrl":"10.34133/research.0615","url":null,"abstract":"<p><p>The rapid evolution of deep learning has markedly enhanced protein-biomolecule binding site prediction, offering insights essential for drug discovery, mutation analysis, and molecular biology. Advancements in both sequence-based and structure-based methods demonstrate their distinct strengths and limitations. Sequence-based approaches offer efficiency and adaptability, while structure-based techniques provide spatial precision but require high-quality structural data. Emerging trends in hybrid models that combine multimodal data, such as integrating sequence and structural information, along with innovations in geometric deep learning, present promising directions for improving prediction accuracy. This perspective summarizes challenges such as computational demands and dynamic modeling and proposes strategies for future research. The ultimate goal is the development of computationally efficient and flexible models capable of capturing the complexity of real-world biomolecular interactions, thereby broadening the scope and applicability of binding site predictions across a wide range of biomedical contexts.</p>","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0615"},"PeriodicalIF":11.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11848751/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143493447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ResearchPub Date : 2025-02-21eCollection Date: 2025-01-01DOI: 10.34133/research.0622
Junjie Zhang, Kaiyuan Tang, Yongbin Yang, Dongliang Yang, Wenpei Fan
{"title":"Advanced Nanoprobe Strategies for Imaging Macrophage Polarization in Cancer Immunology.","authors":"Junjie Zhang, Kaiyuan Tang, Yongbin Yang, Dongliang Yang, Wenpei Fan","doi":"10.34133/research.0622","DOIUrl":"10.34133/research.0622","url":null,"abstract":"<p><p>Macrophages are ubiquitous within the human body and serve pivotal roles in immune surveillance, inflammation, and tissue homeostasis. Phenotypic plasticity is a hallmark of macrophages, allowing their polarization into distinct phenotypes M1 (pro-inflammatory, anti-tumor) and M2 (anti-inflammatory, pro-tumor) in response to local microenvironmental cues. In tumor tissues, the polarization of tumor-associated macrophages profoundly shapes the tumor microenvironment, influencing tumor progression, immune evasion, and metastasis. Therefore, the ability to image and monitor macrophage polarization is essential for comprehending tumor biology and optimizing therapeutic strategies. With the rapid advancement of nanomedicine, a diverse array of nanoprobes has been engineered to specifically target tumor-associated macrophages, offering new avenues for noninvasive in vivo imaging and real-time monitoring of macrophage dynamics within the tumor microenvironment. This perspective highlights recent advancements in macrophage-targeting nanoprobes for imaging macrophage polarization both in vitro and in vivo. It also addresses the current challenges in the field, such as enhancing probe sensitivity, specificity, and biocompatibility, while outlining the future directions for the development of next-generation nanoprobes aimed at precision oncology.</p>","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0622"},"PeriodicalIF":11.0,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11842672/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A Systematic Study on Digital Light Processing 3D Printing of 0-3 Ceramic Composites for Piezoelectric Metastructures.","authors":"Huiru Wang, Qingbo Lai, Dingcong Zhang, Xin Li, Jiayi Hu, Hongyan Yuan","doi":"10.34133/research.0595","DOIUrl":"10.34133/research.0595","url":null,"abstract":"<p><p>Digital light processing (DLP) is a high-speed, high-precision 3-dimensional (3D) printing technique gaining traction in the fabrication of ceramic composites. However, when printing 0-3 composites containing lead zirconate titanate (PZT) particles, a widely used piezoelectric ceramic, severe density and refractive index mismatches between the 2 phases pose challenges for ink synthesis and the printing process. Here, we systematically and quantitatively optimized DLP printing of PZT composites, streamlining process development and providing a solid theoretical and experimental foundation for broader applications of DLP technology. PZT particles were pretreated with air plasma to improve slurry uniformity and enhance stress transfer at the composite interface, leading to improved chemical modification, mechanical strength, and piezoelectric properties. We investigated the effects of key process parameters on printability and accuracy by analyzing the curing behavior of PZT-polymer composites. A quantitative model of the DLP curing process was introduced. Unlike stereolithography (SLA), DLP curing depth was found to depend on energy dose and light intensity, with higher intensities proving more favorable for printing 0-3 PZT composites. From depth/width-energy curves, optimal process parameters were determined. We designed and fabricated a soft piezoelectric metamaterial-based touch sensor using these parameters, achieving a customized output profile. This work offers critical insights into optimizing DLP for functional materials and expands the potential of 3D-printed piezoelectric composites.</p>","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0595"},"PeriodicalIF":11.0,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11842675/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}