Reproductive Medicine and Biology最新文献_第5页

Association Between Hysteroscopic Treatment of Cesarean Scar Disorder and Changes in the Endometrial Microbiome and Clinical Outcomes: A Prospective Observational Study. 宫腔镜治疗剖宫产瘢痕障碍与子宫内膜微生物组变化和临床结果的关系：一项前瞻性观察研究。

IF 4.3 3区医学

Reproductive Medicine and Biology Pub Date : 2026-06-11 eCollection Date: 2026-01-01 DOI: 10.1002/rmb2.70067

Emrullah Sezer, Arif Serhan Cevrioglu, Elif Ozozen, Mehmet Koroglu, Hilal Uslu Yuvaci, Mehmet Musa Aslan, Mehmet Suhha Bostanci, Nermin Akdemir

{"title":"Association Between Hysteroscopic Treatment of Cesarean Scar Disorder and Changes in the Endometrial Microbiome and Clinical Outcomes: A Prospective Observational Study.","authors":"Emrullah Sezer, Arif Serhan Cevrioglu, Elif Ozozen, Mehmet Koroglu, Hilal Uslu Yuvaci, Mehmet Musa Aslan, Mehmet Suhha Bostanci, Nermin Akdemir","doi":"10.1002/rmb2.70067","DOIUrl":"10.1002/rmb2.70067","url":null,"abstract":"Purpose: To evaluate the association between hysteroscopic treatment of cesarean scar disorder (CSDi), clinical improvement, and changes in the endometrial microbiome, as this remains unclear.Methods: This prospective observational study included 40 women diagnosed with symptomatic CSDi. Clinical outcomes, including symptom severity and ultrasonographic measurements, were assessed before and after hysteroscopic treatment. Endometrial microbiome analysis using 16S rRNA gene sequencing was performed in a predefined subgroup of 22 patients preoperatively and at 3 months postoperatively. Microbial composition and diversity were analyzed and correlated with clinical findings.Results: Hysteroscopic treatment resulted in a significant reduction in isthmocele dimensions and improvement in clinical symptoms across the entire cohort (p < 0.01). In the subgroup undergoing microbiome analysis, postoperative samples demonstrated a shift from a polymicrobial profile consisting mostly of Clostridia and Bacteroidia to a Lactobacillus-dominant microbial profile, accompanied by a decrease in microbial diversity (p < 0.001). These microbiological changes occurred in parallel with clinical improvement.Conclusions: Hysteroscopic treatment of CSDi is associated with favorable clinical outcomes and concurrent alterations in the endometrial microbiome. Although microbiome analyses were performed in a subset of patients, the findings suggest a potential relationship between anatomical correction and microbial environment. Larger, controlled studies are warranted to further elucidate this association.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"25 1","pages":"e70067"},"PeriodicalIF":4.3,"publicationDate":"2026-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13260867/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148252919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to "Autoimmune Thyroid Disease and Female Fertility: Clinical Evidence on Ovarian Reserve, Infertility Treatment Outcomes, and Pathophysiology". 对“自身免疫性甲状腺疾病和女性生育能力：卵巢储备、不孕症治疗结果和病理生理学的临床证据”的更正。

IF 3.3 3区医学

Reproductive Medicine and Biology Pub Date : 2026-06-10 eCollection Date: 2026-01-01 DOI: 10.1002/rmb2.70065

引用次数: 0

Real-World Data on the Use of Follitropin Delta in a Dutch IVF Center. 荷兰试管婴儿中心使用follitoppin Delta的实际数据。

IF 4.3 3区医学

Reproductive Medicine and Biology Pub Date : 2026-06-05 eCollection Date: 2026-01-01 DOI: 10.1002/rmb2.70050

Pauline Boom, Eva C Creutzberg, Maria P H Koster, Joop S E Laven

{"title":"Real-World Data on the Use of Follitropin Delta in a Dutch IVF Center.","authors":"Pauline Boom, Eva C Creutzberg, Maria P H Koster, Joop S E Laven","doi":"10.1002/rmb2.70050","DOIUrl":"10.1002/rmb2.70050","url":null,"abstract":"Research question: How does follitropin delta administered with an individualized dose based on body weight and AMH perform in daily practice, and is this comparable to results from a randomized phase III clinical trial in terms of ovarian response, pregnancy, and safety?Design: This is a descriptive analysis of real-world data obtained from the Erasmus University Medical Centre (EMC). Patients were treated with the dose of follitropin delta calculated according to the algorithm described in the ESTHER-1 trial. Outcome data were restricted to the first IVF cycle in patients with a regular ovulatory cycle treated with a GnRH antagonist protocol for whom the intended day of embryo transfer was day 5.Results: The ongoing pregnancy rate was 30.8% per started cycle, which is equivalent to the ESTHER-1 trial. The number of oocytes retrieved (10.3 ± 5.4) was also comparable. All other ovarian response parameters were within the range that was expected on the basis of the results of the ESTHER-1 trial.Conclusions: The use of follitropin delta in a real-world clinical setting results in ovarian response outcomes comparable in range to those in the follitropin delta arm of the ESTHER-1 registration trial. No differences were observed in treatment outcomes.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"25 1","pages":"e70050"},"PeriodicalIF":4.3,"publicationDate":"2026-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13238530/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148199942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transfer Strategy Minimizing Twin Risk of Double Blastocyst Transfers Based on Prediction Models. 基于预测模型的双囊胚移植风险最小化转移策略。

IF 4.3 3区医学

Reproductive Medicine and Biology Pub Date : 2026-05-28 eCollection Date: 2026-01-01 DOI: 10.1002/rmb2.70064

Kazuma Onishi, Daichi Inoue, Yuta Kida, Masae Kojima, Noritaka Fukunaga, Yoshimasa Asada

{"title":"Transfer Strategy Minimizing Twin Risk of Double Blastocyst Transfers Based on Prediction Models.","authors":"Kazuma Onishi, Daichi Inoue, Yuta Kida, Masae Kojima, Noritaka Fukunaga, Yoshimasa Asada","doi":"10.1002/rmb2.70064","DOIUrl":"10.1002/rmb2.70064","url":null,"abstract":"Purpose: To develop a simulation model to guide single embryo transfer (SET) versus double embryo transfer (DET) decisions by minimizing twin risk per transfer while maintaining treatment efficiency.Methods: This retrospective observational study included individuals undergoing SET or DET with frozen blastocysts in hormone replacement therapy. Three generalized estimating equation-based models were developed: ongoing pregnancy after SET (Model 1) and DET (Model 2), and twin pregnancy after DET (Model 3). Model 1 was constructed using propensity score-matched SETs comparable to DETs. The simulation aimed to identify the optimal transfer sequence for patients with multiple blastocysts using a stepwise optimization that selects SET or DET at each transfer while maximizing pregnancy probability and constraining twin risk below predefined thresholds.Results: 5721 DET and 5719 matched SET cycles were included in the analysis. The ongoing pregnancy rate was 20.1% in matched SET and 25.1% in DET, while the ongoing twin pregnancy rate following DET was 5.5%. Area under the curve was 0.77, 0.73, and 0.82 for Model 1-3, respectively. Simulation demonstrated an optimal sequence combining SET/DET under the individualized twin risk in a variety of scenarios (https://sites.google.com/view/det-simulator/).Conclusions: This model supports individualized embryo transfer planning by balancing treatment efficiency and twin risk.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"25 1","pages":"e70064"},"PeriodicalIF":4.3,"publicationDate":"2026-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13239488/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148199917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hyperosmotic HEPES-CZB Enhances Post-ICSI Survival of C57BL/6N Mouse Oocytes. 高渗HEPES-CZB提高C57BL/6N小鼠卵母细胞icsi后的存活率。

IF 4.3 3区医学

Reproductive Medicine and Biology Pub Date : 2026-05-25 eCollection Date: 2026-01-01 DOI: 10.1002/rmb2.70063

Kango Yamaji, Rei Inoue, Sayaka Wakayama, Daiyu Ito, Teruhiko Wakayama

{"title":"Hyperosmotic HEPES-CZB Enhances Post-ICSI Survival of C57BL/6N Mouse Oocytes.","authors":"Kango Yamaji, Rei Inoue, Sayaka Wakayama, Daiyu Ito, Teruhiko Wakayama","doi":"10.1002/rmb2.70063","DOIUrl":"10.1002/rmb2.70063","url":null,"abstract":"Purpose: This study aimed to determine whether performing intracytoplasmic sperm injection (ICSI) under transiently increased osmolality in HEPES-CZB can improve post-ICSI survival as well as embryo and offspring production in C57BL/6N mice.Methods: HEPES-CZB was supplemented with 0-200 mM NaCl or KCl, or 0-400 mM D(+)-glucose or D(-)-mannitol. Oocytes were transferred to each hyperosmotic medium and then subjected to ICSI. Post-ICSI survival, normal fertilization, and blastocyst development were evaluated as rates per injected oocytes. Blastocyst quality was examined by immunostaining to quantify cell types and numbers. Two-cell embryos generated under the best conditions were transferred to evaluate full-term development.Results: Across supplements, increased osmolality consistently reduced oocyte diameter. Following ICSI, the survival rate of these oocytes was significantly increased, and development to the blastocyst stage was maximized under conditions of 100 mM NaCl, 100 mM KCl, 150 mM glucose, and 200 mM mannitol. Under these conditions, the full-term development per injected oocyte remained comparable to the control.Conclusion: Hyperosmotic ICSI significantly improves post-ICSI survival but may compromise developmental competence at higher concentrations, resulting in no net increase in offspring production. Further optimization may increase the number of embryos produced using this approach and reduce workload on the embryologist.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"25 1","pages":"e70063"},"PeriodicalIF":4.3,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13240058/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148199887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revisiting the Trigger-to-Oocyte Retrieval Interval: A Machine Learning-Guided Strategy for Improving Oocyte Yield in Diminished Ovarian Reserve. 重新审视触发到卵母细胞的检索间隔：在减少卵巢储备中提高卵母细胞产量的机器学习指导策略。

IF 4.3 3区医学

Reproductive Medicine and Biology Pub Date : 2026-05-18 eCollection Date: 2026-01-01 DOI: 10.1002/rmb2.70058

Yoko Suzuki, Ying Liu, Michihiro Tanikawa, Yasushi Hirota, Yanpin Kuang

{"title":"Revisiting the Trigger-to-Oocyte Retrieval Interval: A Machine Learning-Guided Strategy for Improving Oocyte Yield in Diminished Ovarian Reserve.","authors":"Yoko Suzuki, Ying Liu, Michihiro Tanikawa, Yasushi Hirota, Yanpin Kuang","doi":"10.1002/rmb2.70058","DOIUrl":"10.1002/rmb2.70058","url":null,"abstract":"Purpose: In IVF, the trigger-to-oocyte retrieval interval (TOI) is typically 34-36 h to avoid spontaneous ovulation. In women with diminished ovarian reserve (DOR) or advanced age, standard protocols often result in premature ovulation or empty follicles. Nonsteroidal anti-inflammatory drugs (NSAIDs) can extend the ovulatory window, enabling exploration of extending TOI. We aimed to evaluate the impact of TOI and to develop a machine learning model for individualized TOI optimization.Methods: Retrospective study on 15,033 IVF cycles to evaluate the effects of TOI extension (≥ 36 h) on the mature oocyte rate (MOR) and oocyte retrieval rate (ORR). Seven machine learning models were trained to predict matured oocytes counts (MII) and retrieved oocyte count (Oocount), then calculate for MOR and ORR as the primary endpoints, following external validation of 387 unseen cases.Results: TOI extension significantly improved MOR p = 0.00034 and ORR p = 0.0051 in POSEIDON groups 3 and 4. Extreme gradient boosting (XGBoost) model exhibited the best predictive accuracy. In external validation, R 2 decreased while MAE and RMSE remained.Conclusions: TOI extension enhances both MOR and ORR in DOR patients. Machine learning models reliably predict MII and Oocount, enabling the calculation of MOR and ORR across different TOIs and facilitating the optimal retrieval timing.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"25 1","pages":"e70058"},"PeriodicalIF":4.3,"publicationDate":"2026-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13184380/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147982976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Effectiveness of Follitropin Delta, Follitropin Alpha, and hMG in ART Cycles: A Single-Center Retrospective Cohort Study With Propensity Score Matching. 促卵泡素δ、促卵泡素α和hMG在ART周期中的比较有效性：一项倾向评分匹配的单中心回顾性队列研究。

IF 4.3 3区医学

Reproductive Medicine and Biology Pub Date : 2026-05-13 eCollection Date: 2026-01-01 DOI: 10.1002/rmb2.70060

Noritoshi Enatsu, Kunihiro Enatsu, Yihsien Enatsu, Ai Yamada, Koji Yamada, Nao Hayashi, Eri Okamoto, Shoji Kokeguchi, Hiroaki Shibahara, Masahide Shiotani

{"title":"Comparative Effectiveness of Follitropin Delta, Follitropin Alpha, and hMG in ART Cycles: A Single-Center Retrospective Cohort Study With Propensity Score Matching.","authors":"Noritoshi Enatsu, Kunihiro Enatsu, Yihsien Enatsu, Ai Yamada, Koji Yamada, Nao Hayashi, Eri Okamoto, Shoji Kokeguchi, Hiroaki Shibahara, Masahide Shiotani","doi":"10.1002/rmb2.70060","DOIUrl":"10.1002/rmb2.70060","url":null,"abstract":"Objective: To compare clinical outcomes of ART cycles stimulated with follitropin delta, follitropin alpha, or human menopausal gonadotropin (hMG) using a single-center retrospective cohort with propensity score matching (PSM).Methods: We analyzed 20 809 ART cycles (2021-2024). PSM adjusted for age, body weight, anti-Müllerian hormone, antral follicle count, prior ART attempts, stimulation protocol, and trigger method. The matched cohort comprised 7,647 cycles (2549/group). The primary outcome was oocyte yield. Secondary outcomes included blastocyst development, ovarian enlargement ≥ 5 cm, and clinical pregnancy rates.Results: The primary outcome regarding oocyte yield was statistically indeterminate across different analytical approaches, and the observed differences were clinically marginal. Both recombinant FSH preparations showed higher blastocyst yields and a lower observed incidence of ovarian enlargement ≥ 5 cm compared with hMG; the latter finding, however, does not establish a clinical safety advantage because clinically diagnosed OHSS was not evaluated. Clinical pregnancy rates were comparable among the three groups.Conclusions: Differences in oocyte yield among follitropin delta, follitropin alpha, and hMG were small and statistically inconsistent. Secondary findings should be interpreted as exploratory, suggesting broadly comparable clinical performance among the three gonadotropin preparations.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"25 1","pages":"e70060"},"PeriodicalIF":4.3,"publicationDate":"2026-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13172277/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147964633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endobiota-Estrobolome Profiles in Reproductive-Aged Women With Ovarian Endometriosis. 卵巢子宫内膜异位症的育龄妇女的内源性雌激素谱。

IF 4.3 3区医学

Reproductive Medicine and Biology Pub Date : 2026-05-12 eCollection Date: 2026-01-01 DOI: 10.1002/rmb2.70061

Chia-Yu Hu, Angel Hsin-Yu Pai, Yi-Wen Wang, Pei-Chen Lu, Hsien-Ming Wu, Hong-Yuan Huang

{"title":"Endobiota-Estrobolome Profiles in Reproductive-Aged Women With Ovarian Endometriosis.","authors":"Chia-Yu Hu, Angel Hsin-Yu Pai, Yi-Wen Wang, Pei-Chen Lu, Hsien-Ming Wu, Hong-Yuan Huang","doi":"10.1002/rmb2.70061","DOIUrl":"10.1002/rmb2.70061","url":null,"abstract":"Purpose: This case-control study investigated whether ovarian endometriosis is associated with altered estrogen metabolism and gut or urogenital microbiota by analyzing enzyme activity, bacterial composition, and variations of estrogen metabolites in fecal, vaginal, and urinary samples.Methods: Thirty-eight reproductive-aged women were enrolled, including 24 with pathologically confirmed ovarian endometriosis and 14 controls. Stool, urine, and vaginal samples were collected preoperatively. Gut β-glucuronidase and β-glucosidase activities were measured, estrogen and 14 metabolites were quantified using liquid chromatography-mass spectrometry, and gut and vaginal microbiota were analyzed by 16S rRNA gene sequencing. Microbial composition, diversity, and abundance were compared between groups.Results: Gut β-glucuronidase activity and overall microbial diversity were comparable between groups; however, the control group showed a higher prevalence of the genus Rothia, whereas several genera, including Megamonas and [Eubacterium] coprostanoligenes_group, were enriched in the ovarian endometriosis group. In contrast, vaginal samples from patients with ovarian endometriosis demonstrated significantly reduced bacterial abundance and diversity, accompanied by lower levels of 4-methoxyestrone, 2-methoxyestrone, and 2-hydroxyestrone-3-methyl ether.Conclusions: Although overt dysbiosis was not observed, specific microbial shifts and altered estrogen metabolites may reflect disturbances in estrogen metabolism and urogenital-gastrointestinal microbiota in ovarian endometriosis.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"25 1","pages":"e70061"},"PeriodicalIF":4.3,"publicationDate":"2026-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13168752/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147942756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel Insights into Normal Karyotype Miscarriage: Distinctive Patterns and Implications for Diagnosis Based on Next-Generation Sequencing Analysis. 对正常核型流产的新见解：基于下一代测序分析的独特模式和诊断意义。

IF 4.3 3区医学

Reproductive Medicine and Biology Pub Date : 2026-05-11 eCollection Date: 2026-01-01 DOI: 10.1002/rmb2.70053

Yumene Kubota, Tomoko Ichikawa, Shigeru Matsuda, Yoshimitsu Kuwabara, Shunji Suzuki, Yasuyuki Negishi

{"title":"Novel Insights into Normal Karyotype Miscarriage: Distinctive Patterns and Implications for Diagnosis Based on Next-Generation Sequencing Analysis.","authors":"Yumene Kubota, Tomoko Ichikawa, Shigeru Matsuda, Yoshimitsu Kuwabara, Shunji Suzuki, Yasuyuki Negishi","doi":"10.1002/rmb2.70053","DOIUrl":"10.1002/rmb2.70053","url":null,"abstract":"Purpose: This study aimed to identify the clinical differences between normal and abnormal karyotype miscarriages and evaluate whether clinical findings could predict the presence or absence of chromosomal abnormalities.Methods: A total of 117 patients with early miscarriage who underwent chorionic villus chromosomal testing using next-generation sequencing were retrospectively analyzed. Clinical findings were compared between the normal (euploid; n = 47) and abnormal karyotype (aneuploid; n = 70) groups.Results: Normal karyotype miscarriages occurred significantly more often before yolk sac visualization (29.8% vs. 0%, p < 0.0001) and before fetal heartbeat confirmation (51.1% vs. 15.7%, p = 0.0001) than abnormal karyotype miscarriages. The mean crown-rump length was larger in the normal karyotype group (15.3 ± 8.2 mm vs. 11.8 ± 6.8 mm, p = 0.037). Normal karyotype miscarriages exhibited a bimodal distribution in gestational age at miscarriage. In addition, the normal karyotype was associated with a history of two or more biochemical pregnancy losses (21.3% vs. 7.1%, p = 0.045).Conclusion: Distinct clinical and ultrasonographic patterns were observed in normal and abnormal karyotype miscarriages. These features have limited diagnostic value, and their usefulness in supporting counseling or decision-making is also limited when genetic testing is not available.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"25 ","pages":"e70053"},"PeriodicalIF":4.3,"publicationDate":"2026-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13161466/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147932824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reversibility of Spermatogonial Stem Cell Injury After Single Acute Scrotal Hyperthermia in Mouse and Rat Models: A Systematic Review. 小鼠和大鼠单次急性阴囊高温后精原干细胞损伤的可逆性：系统综述。

IF 4.3 3区医学

Reproductive Medicine and Biology Pub Date : 2026-05-11 eCollection Date: 2026-01-01 DOI: 10.1002/rmb2.70055

Rashid A Aldahhan

{"title":"Reversibility of Spermatogonial Stem Cell Injury After Single Acute Scrotal Hyperthermia in Mouse and Rat Models: A Systematic Review.","authors":"Rashid A Aldahhan","doi":"10.1002/rmb2.70055","DOIUrl":"10.1002/rmb2.70055","url":null,"abstract":"Background: Acute scrotal hyperthermia is a recognized spermatogenesis stressor, but recovery of spermatogonial stem cells after a single mild heat exposure remains unclear, particularly over cycle-relevant timeframes. This systematic review evaluates the in vivo local scrotal hyperthermia across at least one full spermatogenic cycle.Methods: A PICO-driven search identified 18 mouse and rat studies using a single scrotal heating exposure. Outcomes were synthesized thematically across structural, cellular, endocrine/apoptotic, and sperm domains.Results: Injury patterns were graded and temperature- and species-dependent, with more consistent abnormalities at 43°C and milder effects in rats than in mice. Structural endpoints often remained abnormal at the longest follow-up, and architectural repair did not reliably achieve functional recovery. Mouse stereology at 43°C indicated long-term SSC and germ-cell depletion, with Leydig-cell and partial Sertoli-cell losses; rat stereology was sparse and absent at 42°C. Testosterone responses were heterogeneous, whereas tubular apoptosis was more consistently elevated in mice at 43°C. Sperm concentration/count declined where assessed, with more frequent impairments of motility and other quality indices, including DNA integrity, at 43°C.Conclusion: Recovery was often incomplete and endpoint-specific, with impairment more consistently detected at 43°C, particularly in mice. Future studies require standardized thermal dosing, transparent bias-mitigation, and SSC/niche-resolved functional assays.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"25 ","pages":"e70055"},"PeriodicalIF":4.3,"publicationDate":"2026-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13160925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147932842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0