Molecular Ecology最新文献

{"title":"Islands Promote Diversification of the Silvereye Species Complex: A Phylogenomic Analysis of a Great Speciator","authors":"Andrea Estandía,&nbsp;Nilo Merino Recalde,&nbsp;Ashley T. Sendell-Price,&nbsp;Dominique A. Potvin,&nbsp;William Goulding,&nbsp;Bruce C. Robertson,&nbsp;Sonya Clegg","doi":"10.1111/mec.17830","DOIUrl":"10.1111/mec.17830","url":null,"abstract":"<p>Geographic isolation plays a pivotal role in speciation by restricting gene flow between populations through distance or physical barriers. However, the speciation process is complex, influenced by the interplay between dispersal ability and geographic isolation, as seen in “great speciators” – bird species that simultaneously have broad island distributions but high levels of subspecific diversity. Comparing genomic population differentiation in species that occupy both continental and island settings can reveal the effects of different forms of geographic isolation and validate if the primary mechanism proposed to catalyse a great speciator pattern, that is, dispersal reduction following island colonisation, has occurred. The highly diverse white-eye family Zosteropidae includes several great speciators, including the silvereye (<i>Zosterops lateralis</i>), with 16 subspecies (11 occurring on islands), distributed on the Australian continent and numerous southwest Pacific islands. We compared continental and island patterns of divergence using whole genome and morphological data. Australian mainland populations showed a low genetic population structure, lack of isolation by distance patterns and low morphological diagnosability, suggesting that the species' dispersal propensity in a continental setting is sufficient to overcome multiple forms of geographic barriers and large geographic distances. In contrast, except for island populations less than 200 years old, most island populations were highly genomically structured with clearer morphological diagnosability even if separated by relatively short geographic distances. The inferred reduction of dispersal propensity in island situations is consistent with the proposed model of great speciator formation on islands. Our phylogenomic analyses also allowed resolution of the silvereyes' evolutionary position, showing their relatively early emergence (~1.5 Mya) within the rapidly radiating Zosteropidae, while population-level analyses demonstrated where morphological subspecies and genomic data align and disagree. However, the silvereye example also shows how uncertainties about relationships remain when reconstructing evolutionary history in rapidly radiating groups, even when whole genome data is available. Altogether, our results show how within-species genomic and morphological patterns measured over broad spatial scales and with varying geographic contexts can help reveal when particular stages of speciation such as great speciators are likely to emerge.</p>","PeriodicalId":210,"journal":{"name":"Molecular Ecology","volume":"34 14","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/mec.17830","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144264955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DDM1 Controls Heritable Natural Epigenetic Variation in Arabidopsis","authors":"Peng Zhang,&nbsp;Datong Xing,&nbsp;Muhammad Shahbaz Chishti,&nbsp;Linan Xie,&nbsp;Qingzhu Zhang","doi":"10.1111/mec.17828","DOIUrl":"10.1111/mec.17828","url":null,"abstract":"<div>\u0000 \u0000 <p>As a part of epigenetic modifications, DNA methylation involves the addition of a methyl group to cytosine. In plants, this process occurs in three sequence contexts (CG, CHG and CHH) through two pathways: de novo DNA methylation and DNA methylation maintenance. DNA methylation is highly conserved between ecotypes due to its heritability and role in genomic stability. However, numerous naturally occurring differentially methylated regions (NMRs) exist between ecotypes, which are also heritable and contribute to differential gene expression and phenotypic variation. Decreased DNA methylation observed in mutants of RdDM or DNA methylation maintenance pathways highlights the potential roles of these pathways in NMR formation and inheritance. Nevertheless, the complexity of plant DNA methylation across different contexts and pathways has made the contributions of these two pathways in NMR formation and inheritance remain unclear. To address this, we chose two <i>Arabidopsis thaliana</i> ecotypes, Col-0 (Col) and C24, and utilised mutants of <i>pol4/5</i> and <i>ddm1</i> in both ecotypes. By examining the changes in NMRs within these mutants, we aimed to elucidate the roles of DNA methylation pathways in NMR formation and inheritance. Our results revealed (1) NMRs can be categorised into three types, but classification is not solely based on methylation contexts; (2) compared to RdDM, the DNA methylation maintenance pathway plays a more dominant role in NMR formation and is less influenced by SNPs; (3) DNA methylation maintenance, rather than RdDM, controls NMR inheritance. Our findings highlight the role of the DNA methylation maintenance pathway in NMR formation and inheritance.</p>\u0000 </div>","PeriodicalId":210,"journal":{"name":"Molecular Ecology","volume":"34 14","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144264883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assigning Phenologically Asynchronous Moths to Source Populations Using Individual Genotypes","authors":"Patrick M. A. James,&nbsp;Julia T. Fang,&nbsp;Julian Wittische,&nbsp;Michel Cusson,&nbsp;Jeremy Larroque,&nbsp;Amanda Roe,&nbsp;Rob Johns","doi":"10.1111/mec.17832","DOIUrl":"10.1111/mec.17832","url":null,"abstract":"<p>The spruce budworm (<i>Choristoneura fumiferana</i>; SBW) is a periodically outbreaking forest insect pest that affects the boreal forests of North America through extensive defoliation and tree mortality. Causes of widespread spatial synchrony of SBW outbreaks remain a key question in the ecology and management of this species. While the Moran effect (correlated favourable environmental conditions) and density-dependent dispersal (from epicentres of demographic explosions) have been proposed and supported as drivers of synchronised outbreaks, the relative contribution of long-distance dispersal is still poorly understood. In this study, we use a novel approach to distinguish resident from migrant moths and to assign migrants to likely source clusters with the goal of better characterising regional dispersal. First, we characterise the genetic diversity and structure of resident SBW larvae and three phenologically separated groups of moths over one flight season using Genotyping-by-Sequencing. Then, using a novel machine learning approach, we assign putative migrants to their likely source populations. We hypothesised that migrant moths and resident larvae would be genetically distinct and could be assigned to source populations. Our findings revealed complex patterns of moth dispersal and population differentiation within a single season, including two spatially overlapping genetic clusters. We observed subtle but significant genetic differences between resident larvae and migrant moths, supporting the hypothesis that long-distance dispersal contributes to outbreak dynamics and synchrony. These insights enhance our understanding of SBW population dynamics and suggest that effective management strategies, such as the Early Intervention Strategy (EIS), must account for the role of dispersal in mitigating the detrimental effects of major outbreaks.</p>","PeriodicalId":210,"journal":{"name":"Molecular Ecology","volume":"34 14","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/mec.17832","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144264882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-Wide Adaptation to a Complex Environmental Gradient in a Keystone Phytoplankton Species","authors":"Eveline Pinseel,&nbsp;Elizabeth C. Ruck,&nbsp;Teofil Nakov,&nbsp;Per R. Jonsson,&nbsp;Olga Kourtchenko,&nbsp;Anke Kremp,&nbsp;Matthew I. M. Pinder,&nbsp;Wade R. Roberts,&nbsp;Conny Sjöqvist,&nbsp;Mats Töpel,&nbsp;Anna Godhe,&nbsp;Matthew W. Hahn,&nbsp;Andrew J. Alverson","doi":"10.1111/mec.17817","DOIUrl":"10.1111/mec.17817","url":null,"abstract":"<p>Marine phytoplankton play essential roles in global primary production and biogeochemical cycles. Yet, the evolutionary genetic underpinnings of phytoplankton adaptation to complex marine and coastal environments, where many environmental variables fluctuate and interact, remain unclear. We combined population genomics with experimental transcriptomics to investigate the genomic basis underlying a natural evolutionary experiment that has played out over the past 8000 years in one of the world's largest brackish water bodies: the colonisation of the Baltic Sea by the ancestrally marine diatom <i>Skeletonema marinoi</i>. To this end, we combined target capture of the entire nuclear genome with pooled shotgun sequencing, and showed that the method performs well on both cultures and single cells. Genotype–environment association analyses identified &gt; 1000 genes with signals of selection in response to major environmental gradients in the Baltic Sea, which apart from salinity, include marked differences in temperature and nutrient supply. Locally adapted genes were related to diverse metabolic processes, including signal transduction, cell cycle, DNA methylation and maintenance of homeostasis. The locally adapted genes showed significant overlap with salinity-responsive genes identified in a laboratory common garden experiment, suggesting the Baltic salinity gradient contributes to local adaptation of <i>S. marinoi</i>. Taken together, our data show that local adaptation of phytoplankton to complex coastal environments, which are characterised by a multitude of environmental gradients, is driven by widespread changes in diverse metabolic pathways and functions.</p>","PeriodicalId":210,"journal":{"name":"Molecular Ecology","volume":"34 13","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/mec.17817","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144256982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dispersal, Isolation and Local Adaptation Promote Speciation in South American Savannas as Indicated by a Phylogenomic Analysis of a Passerine","authors":"Alejandro Manuel Ferreiro,&nbsp;Renato Caparroz,&nbsp;Alexandre Aleixo,&nbsp;Luís Fábio Silveira,&nbsp;Samira Rezende Duarte,&nbsp;Cássia Alves Lima-Rezende,&nbsp;Martín Carboni,&nbsp;Gustavo Sebastián Cabanne","doi":"10.1111/mec.17827","DOIUrl":"10.1111/mec.17827","url":null,"abstract":"<div>\u0000 \u0000 <p>South American savannas are a disjunct biome with an unclear evolutionary history. We tested hypotheses about their Quaternary history and the evolution of savanna cores through fragmentation or dispersal from the Cerrado. We used genomic data (genotyping-by-sequencing) and ecological niche models of the Burnished-buff Tanager (<i>Stilpnia cayana</i> Linnaeus 1766) to evaluate intraspecific differentiation, gene flow, past range shifts and landscape genomics association. We found clear genomic differences between populations on each side of the Amazon basin and high admixture in Marajó Island and Bolivia. Landscape genomics analysis indicated that the Amazon River, isolation by distance and temperature predict genomic differentiation in this bird. Taken together, the results suggest that a combination of dispersal from the Cerrado, isolation due to geographic distance, and the Amazon River basin, and local adaptation shaped species diversification.</p>\u0000 </div>","PeriodicalId":210,"journal":{"name":"Molecular Ecology","volume":"34 13","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144264884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome Analyses Reveal Diverse Riverine Genetic Contributions to the Lake Malawi Cichlid Radiation","authors":"Sophie Gresham,&nbsp;Bosco Rusuwa,&nbsp;Maxon Ngochera,&nbsp;George F. Turner,&nbsp;Martin J. Genner,&nbsp;Milan Malinsky,&nbsp;Hannes Svardal","doi":"10.1111/mec.17786","DOIUrl":"10.1111/mec.17786","url":null,"abstract":"<p>Comparative studies of whole genomes have increasingly shown that genetic introgression between closely related species is surprisingly common across the tree of life, making the description of biodiversity and understanding the process of speciation complex and challenging. The adaptive radiation of cichlid fishes in Lake Malawi, that is characterised by hybrid origins and cases of recent introgression, provides a valuable model system to study the evolutionary implications of introgression. However, many potential sources of introgression into the radiation have not yet been investigated. Here we use whole genome data from 239 species from Lake Malawi and 76 species from surrounding African river and lake systems to identify previously unknown introgression events involving the Malawi radiation. Computing genome-wide excess allele sharing (ABBA-BABA statistics) and window-based statistics, we find that three independent riverine cichlid lineages show significantly higher allele sharing with the Malawi radiation than expected, suggesting historical genetic exchange. Introgressed haplotypes are distributed relatively uniformly across the Malawi radiation, indicating that most hybrid-derived polymorphism was acquired and sorted before the formation of the contemporary Malawi radiation. Our results point towards several previously unknown contributors to the Malawi cichlid hybrid swarm and show that the history of one of the largest vertebrate radiations is more complex than previously thought.</p>","PeriodicalId":210,"journal":{"name":"Molecular Ecology","volume":"34 13","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/mec.17786","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144264954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contrasting Evolutionary Trajectories Under Paternal Genome Elimination in Male and Female Citrus Mealybugs","authors":"Andrew J. Mongue,&nbsp;Tamsin E. Woodman,&nbsp;Hollie Marshall,&nbsp;Arkadiy Garber,&nbsp;José C. Franco,&nbsp;John P. McCutcheon,&nbsp;Laura Ross","doi":"10.1111/mec.17826","DOIUrl":"10.1111/mec.17826","url":null,"abstract":"<p>Most studies of sex-biased genes explore their evolution in familiar chromosomal sex determination systems, leaving the evolution of sex differences under alternative reproductive systems unknown. Here we explore the system of paternal genome elimination employed by mealybugs (Hemiptera: Pseudococcidae) which have no sex chromosomes. Instead, all chromosomes are autosomal and inherited in two copies, but sex is determined by the ploidy of expression. Females express both parental alleles, but males reliably silence their paternally inherited chromosomes, creating genome-wide haploid expression in males and diploid expression in females. Additionally, sons do not express alleles directly inherited from their fathers, potentially disrupting the evolution of male-benefiting traits. To understand how these dynamics impact molecular evolution, we generated sex-specific RNAseq, a new gene annotation, and whole-genome population sequencing of the citrus mealybug, <i>Planococcus citri</i>. We found that genes expressed primarily in females hold more variation and evolve more quickly than those expressed in males or both sexes. Conversely, we found more apparent adaptation in genes expressed mainly in males than in those expressed in females. Put together, in this paternal genome elimination system there is slower change on the male side but, by increasing selective scrutiny, an increase in the degree of adaptation in these genes. These results expand our understanding of evolution in a non-Mendelian genetic system and the data we generated should prove useful for future research on this pest insect.</p>","PeriodicalId":210,"journal":{"name":"Molecular Ecology","volume":"34 13","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/mec.17826","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144245453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bumblebee Pollination and Herbivory Alter Genomic Adaptation of Plants to Soil","authors":"Tyler Figueira,&nbsp;Léa Frachon,&nbsp;Florian P. Schiestl","doi":"10.1111/mec.17811","DOIUrl":"10.1111/mec.17811","url":null,"abstract":"<div>\u0000 \u0000 <p>Adaptation to environmental heterogeneity is a key driver of between-population genomic differentiation, yet we know very little about how genomic divergence is affected by adaptation to multiple ecological factors. Using an experimental evolution approach, we whole-genome re-sequenced ‘fast-cycling’ <i>Brassica rapa</i> plants which had evolved during eight generations of selection from different combinations of soil type, aphid herbivore presence or absence, and either bee- or hand pollination. Our results show that bumblebee pollination was the strongest driver of genomic divergence and that the degree of genomic divergence was strongly associated with the number of SNP markers identified in genomic selection scans. Furthermore, we found that the number of SNPs under selection was affected by herbivory in a soil-dependent way. More specifically, aphid herbivory was associated with an increased number of selected SNPs for bee-pollinated plants that evolved in tuff soil but was associated with a decreased number of selected SNPs for bee-pollinated plants that evolved in the more resource-limiting limestone soil. We also found that a higher number of selected SNPs was associated with higher rates of phenotypic evolution for 27 phenotypic traits including morphology and scent. Finally, we found that variation in pleiotropy between treatments was related to both the degree of genomic divergence and the number of SNPs under selection. Our results demonstrate that different soil types promote unique adaptive genomic architectures in response to biotic interactions, thus increasing genomic divergence between plant populations.</p>\u0000 </div>","PeriodicalId":210,"journal":{"name":"Molecular Ecology","volume":"34 13","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144245452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Genomic Signature of Demographic Decline in a Long-Distance Migrant in a Range-Extreme Population","authors":"George Day,&nbsp;Thomas Bolderstone,&nbsp;Greg J. Conway,&nbsp;Tony Cross,&nbsp;Tony Davis,&nbsp;Matilda Dolan,&nbsp;Mervyn Greening,&nbsp;Colin Neale,&nbsp;Ian Nicholson,&nbsp;Kim Nicholson,&nbsp;Ann Ward,&nbsp;Nik Ward,&nbsp;Graeme Fox,&nbsp;Ewan Harney,&nbsp;Helen Hipperson,&nbsp;Kathryn Maher,&nbsp;Jamie Thompson,&nbsp;Rachel Tucker,&nbsp;Dean Waters,&nbsp;Kate L. Durrant,&nbsp;Terry Burke,&nbsp;Jon Slate,&nbsp;Kathryn E. Arnold","doi":"10.1111/mec.17805","DOIUrl":"10.1111/mec.17805","url":null,"abstract":"<p>Migratory birds are inherently vagile, a strategy that may reduce the impacts of habitat loss and fragmentation on genetic diversity. However, specialist resource requirements and range-edge distribution can counteract these benefits. The European nightjar (<i>Caprimulgus europaeus</i>) is a long-distance migratory bird and resource specialist. Like other long-distance migrants, nightjar populations have declined across the British Isles and Northwestern Europe over the past century. With this decline well documented in the British Isles, there is a need to quantify its genetic impacts. We applied full genome resequencing to 60 historic (1841–1980) and 36 contemporary British nightjars. Nightjars exhibited a statistically significant 34.8% loss in heterozygosity and an increase in inbreeding over the last ~180 years, showing a departure from panmixia towards weak spatial structure in the modern population. Such fine-scale structuring in migratory birds is rare. Our results provide a case study of fragmentation's impact on a species with specialist resource requirements at its range limit. Similar demographic declines in nightjars and other long-distance migrants across Northern and Western Europe suggest that genetic patterns seen in the British population may reflect those in other nightjar populations and European avifauna. Whilst our results indicate no immediate conservation concern, they depict a trajectory of declining genetic diversity, increasing inbreeding and genetic structure, potentially shared with other migratory species. Our study highlights the value of applying spatiotemporal population genetics analysis to migratory birds, despite their inherent vagility.</p>","PeriodicalId":210,"journal":{"name":"Molecular Ecology","volume":"34 13","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/mec.17805","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144223864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Admixture Affects the Rate and Repeatability of Experimental Adaptation to a Stressful Environment in Callosobruchus maculatus.","authors":"Amy Springer, Brian Kissmer, Zachariah Gompert","doi":"10.1111/mec.17809","DOIUrl":"https://doi.org/10.1111/mec.17809","url":null,"abstract":"<p><p>Hybridisation and admixture are common in nature and can serve as important sources of adaptive potential by generating novel genotype combinations and phenotypes. However, hybrid incompatibilities can also reduce hybrid fitness. Given the pervasiveness of admixture and its potential role in facilitating adaptation, understanding how admixture influences the rate and repeatability of evolution is critical for advancing our understanding of evolutionary dynamics. Yet, few studies have examined how patterns of evolutionary repeatability in admixed lineages are shaped by strong ecological pressures. In this experiment, we evaluated patterns of evolution and repeatability in admixed and non-admixed cowpea seed beetles (Callosobruchus maculatus) adapting to a novel, stressful host: lentil. Specifically, we asked (1) whether admixture facilitates adaptation to lentil, (2) whether repeatability is greater in admixed or non-admixed lineages, and (3) to what extent repeatability in admixed lineages is driven by selection on globally adaptive alleles versus epistatic effects and hybrid incompatibilities. We found that admixture facilitated adaptation to lentil, and evolutionary rescue-defined as adaptation that prevents population extinction-occurred in all lineages. Evolutionary repeatability was highest in two admixed lineages, though evident across all lineages. Adaptation to lentil appeared largely driven by selection on globally adaptive alleles. Nevertheless, even under conditions of evolutionary rescue in a marginal environment, the purging of hybrid incompatibilities contributed substantially to repeated evolution in admixed lineages.</p>","PeriodicalId":210,"journal":{"name":"Molecular Ecology","volume":" ","pages":"e17809"},"PeriodicalIF":4.5,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144214412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}