Redox Report最新文献_第8页

Redox imbalance in Crohn's disease patients is modulated by Azathioprine. 硫唑嘌呤可调节克罗恩病患者的氧化还原失衡。

IF 3.8 2区生物学

Redox Report Pub Date : 2021-12-01 DOI: 10.1080/13510002.2021.1915665

Mohammad Javad Tavassolifar, Mostafa Changaei, Zahra Salehi, Fatemeh Ghasemi, Moslem Javidan, Mohammad Hossein Nicknam, Mohammad Reza Pourmand

{"title":"Redox imbalance in Crohn's disease patients is modulated by Azathioprine.","authors":"Mohammad Javad Tavassolifar, Mostafa Changaei, Zahra Salehi, Fatemeh Ghasemi, Moslem Javidan, Mohammad Hossein Nicknam, Mohammad Reza Pourmand","doi":"10.1080/13510002.2021.1915665","DOIUrl":"https://doi.org/10.1080/13510002.2021.1915665","url":null,"abstract":"Background: Crohn's disease (CD) is a chronic inflammatory disease without a specific cause. Inflammation in these patients can disturb the oxidants/antioxidants balance and results in oxidative stress that plays a destructive role. This study aimed to evaluate the gene expression of sod1, sod2, cat, nrf2 and gp91phox in CD patients before and after Azathioprine (Aza) consumption.Method: Peripheral bloodmononuclear cells (PBMCs) were separated from CD patients (n= 15, mean age = 33.6 ± 1.8) before and after treatment with Aza and healthy controls (n= 15, mean age = 31.5 ± 1.2). The expression levels of sod1, sod2, cat, nrf2 and gp91phox were measured in byusing real-time qRT-PCR technique.Result: The expression levels of gp91phox (P-value < 0.001), cat (P-value < 0.05), sod1 (P-value < 0.001), nrf2 (P-value < 0.001) were significantly increased compared to control group. Following treatment with Aza, the decreased expression levels of gp91phox (P-value < 0.05), cat (P-value < 0.05), sod1(P-value < 0.001) and nrf2 (P-value < 0.001) were observed in CD patients.Conclusion: Overall, our results showed that prescription of Azathioprine can lead to the altered expression of redox system-related genes in patients with CD.","PeriodicalId":21096,"journal":{"name":"Redox Report","volume":"26 1","pages":"80-84"},"PeriodicalIF":3.8,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/13510002.2021.1915665","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38902502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Can thiol-based redox systems be utilized as parts for synthetic biology applications? 巯基氧化还原系统能否作为合成生物学应用的一部分?

IF 3.8 2区生物学

Redox Report Pub Date : 2021-12-01 DOI: 10.1080/13510002.2021.1966183

Ché S Pillay, Nolyn John

{"title":"Can thiol-based redox systems be utilized as parts for synthetic biology applications?","authors":"Ché S Pillay, Nolyn John","doi":"10.1080/13510002.2021.1966183","DOIUrl":"10.1080/13510002.2021.1966183","url":null,"abstract":"Objectives: Synthetic biology has emerged from molecular biology and engineering approaches and aims to develop novel, biologically-inspired systems for industrial and basic research applications ranging from biocomputing to drug production. Surprisingly, redoxin (thioredoxin, glutaredoxin, peroxiredoxin) and other thiol-based redox systems have not been widely utilized in many of these synthetic biology applications.Methods: We reviewed thiol-based redox systems and the development of synthetic biology applications that have used thiol-dependent parts.Results: The development of circuits to facilitate cytoplasmic disulfide bonding, biocomputing and the treatment of intestinal bowel disease are amongst the applications that have used thiol-based parts. We propose that genetically encoded redox sensors, thiol-based biomaterials and intracellular hydrogen peroxide generators may also be valuable components for synthetic biology applications.Discussion: Thiol-based systems play multiple roles in cellular redox metabolism, antioxidant defense and signaling and could therefore offer a vast and diverse portfolio of components, parts and devices for synthetic biology applications. However, factors limiting the adoption of redoxin systems for synthetic biology applications include the orthogonality of thiol-based components, limitations in the methods to characterize thiol-based systems and an incomplete understanding of the design principles of these systems.","PeriodicalId":21096,"journal":{"name":"Redox Report","volume":"26 1","pages":"147-159"},"PeriodicalIF":3.8,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8366655/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39299669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vitamin D levels and oxidative stress markers in patients hospitalized with COVID-19. COVID-19住院患者的维生素D水平和氧化应激标志物

IF 3.8 2区生物学

Redox Report Pub Date : 2021-12-01 DOI: 10.1080/13510002.2021.1999126

Emilija Atanasovska, Marija Petrusevska, Dragica Zendelovska, Katerina Spasovska, Milena Stevanovikj, Katerina Kasapinova, Kalina Gjorgjievska, Nikola Labachevski

{"title":"Vitamin D levels and oxidative stress markers in patients hospitalized with COVID-19.","authors":"Emilija Atanasovska, Marija Petrusevska, Dragica Zendelovska, Katerina Spasovska, Milena Stevanovikj, Katerina Kasapinova, Kalina Gjorgjievska, Nikola Labachevski","doi":"10.1080/13510002.2021.1999126","DOIUrl":"https://doi.org/10.1080/13510002.2021.1999126","url":null,"abstract":"Background: COVID-19 is characterized by the presence of oxidative stress. Vitamin D status has been reviewed as one of the factors that may affect disease severity. The aim of this study was to assess the relationship between serum vitamin D levels, oxidative stress markers and disease severity in hospitalized COVID-19 patients.Methods: Vitamin D levels were measured in 33 patients with COVID-19. The total antioxidant power and plasma peroxides were determined in serum.Results: Severe COVID-19 patients have lower vitamin D levels (18.39 ± 2.29 ng/mL vs. 28.47 ± 3.05 ng/mL, p < .05) and higher oxidative stress compared to the moderate group. When divided according to serum vitamin D levels, significantly higher values of LDH (604.8 ± 76.98 IU/mL vs. 261.57 ± 47.33 IU/mL) and D-dimer (5978 ± 2028ng/mL vs. 977.7 ± 172 ng/mL) were obtained in the group with vitamin D below 30 ng/mL, followed with significantly higher levels of plasma peroxides (d-ROMs: 414.9 ± 15.82 U.Carr vs. 352.4 ± 18.77 U.Carr; p < .05) and oxidative stress index (OSI: 92.25 ± 6.60 vs. 51.89 ± 6.45; p < .001).Conclusion: The presented data provide a justification to consider vitamin D as an important factor that could ameliorate disease severity through its anti-inflammatory and antioxidant effects.","PeriodicalId":21096,"journal":{"name":"Redox Report","volume":"26 1","pages":"184-189"},"PeriodicalIF":3.8,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8567917/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39850516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Saliva nitrite is higher in male children with autism spectrum disorder and positively correlated with serum nitrate. 患有自闭症谱系障碍的男性儿童唾液中亚硝酸盐含量较高，且与血清硝酸盐呈正相关。

IF 5.2 2区生物学

Redox Report Pub Date : 2021-12-01 DOI: 10.1080/13510002.2021.1959133

Lulu Yao, Huimin Fu, Lu Bai, Wenwen Deng, Fang Xie, Ying Li, Rong Zhang, Xinjie Xu, Ting Wang, Shenghan Lai, Jun Wang

{"title":"Saliva nitrite is higher in male children with autism spectrum disorder and positively correlated with serum nitrate.","authors":"Lulu Yao, Huimin Fu, Lu Bai, Wenwen Deng, Fang Xie, Ying Li, Rong Zhang, Xinjie Xu, Ting Wang, Shenghan Lai, Jun Wang","doi":"10.1080/13510002.2021.1959133","DOIUrl":"10.1080/13510002.2021.1959133","url":null,"abstract":"Objectives: Nitric oxide (NO) plays a vital role in neurological development. As an easily accessible and non-invasive fluid, saliva hasn't been evaluated for nitrite among children with autism spectrum disorder (ASD). This study aims to quantify saliva nitrite and explore its relation with serum NO.Methods: Saliva sampling and pretreatment methods were optimized, followed by NO measurement via chemiluminescence for 126 ASD children and 129 normally developing children (ND).Results: In the ASD group, saliva nitrite was significantly higher than that in the ND, with concentrations of 4.97 ± 3.77 μM and 2.66 ± 2.07 μM (p < 0.0001), respectively. Positive correlation was observed between saliva NO2- and serum NO3- in ASD children, which didn't exist in the ND group. Male children in the ASD group had significantly higher NO than that in boys of the ND group, without significant difference between girls in both groups. Correlation was not found between saliva or serum NO and severity of these ASD children.Discussion: It is reported for the first time that saliva nitrite was positively correlated with serum nitrate in ASD children, with significantly higher NO only in autistic boys. Non-invasive saliva might serve as a predictor of health status of ASD children.","PeriodicalId":21096,"journal":{"name":"Redox Report","volume":"26 1","pages":"124-133"},"PeriodicalIF":5.2,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/90/2f/YRER_26_1959133.PMC8330712.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39255635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ROS-induced dramatic lipid changes in Arabidopsis. ros诱导拟南芥脂质发生剧烈变化。

IF 3.8 2区生物学

Redox Report Pub Date : 2021-12-01 DOI: 10.1080/13510002.2021.2002001

Tianlin Jin, Xue Wang, Zhuying Deng, Xiaofang Liu, Dacheng Liang

{"title":"ROS-induced dramatic lipid changes in Arabidopsis.","authors":"Tianlin Jin, Xue Wang, Zhuying Deng, Xiaofang Liu, Dacheng Liang","doi":"10.1080/13510002.2021.2002001","DOIUrl":"https://doi.org/10.1080/13510002.2021.2002001","url":null,"abstract":"Objectives: The beneficial role of ROS was probably in promoting intercellular communication by modifying membrane constituents [Liang D. A salutary role of reactive oxygen species in intercellular tunnel-mediated communication. Front Cell Dev Biol. 2018;6:2]. We investigated how the membrane lipids were responding to ROS and ROS inhibitors.Methods: To examine how ROS affected the lipid profiles, we used thin-layer chromatography to characterize lipid profiles in Arabidopsis plants. Then, the confocal microscopy imaging was used to confirm the change of membrane lipid in a plasma membrane marker line exposed to ROS and ROS inhibitors.Results: We found the relative contents of most lipids in H2O2-treated Arabidopsis plants were increased in roots, rather than in shoots. The increased fluorescent signal of membrane marker induced by H2O2 was mainly enriched in the conductive parts of roots. Several ROS inhibitors also strongly affected the lipid profiles. Among them, diethyldithiocarbamate (DDC) can progressively change the lipid profiles with treatment going on. Membrane marker signal was mainly accumulated in the root tips and epidermal cells after treatment by DDC.Discussion: H2O2 may enhance intercellular communication by inducing different lipid species in the conductive parts of roots. The lipid profiles were widely responding to various ROS reagents and might play a role in intercellular signaling.","PeriodicalId":21096,"journal":{"name":"Redox Report","volume":"26 1","pages":"190-196"},"PeriodicalIF":3.8,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8583927/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39859368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Redox imbalance induced by docetaxel in the neuroblastoma SH-SY5Y cells: a study of docetaxel-induced neuronal damage. 多西紫杉醇诱导神经母细胞瘤SH-SY5Y细胞氧化还原失衡:多西紫杉醇诱导神经元损伤的研究

IF 3.8 2区生物学

Redox Report Pub Date : 2021-12-01 DOI: 10.1080/13510002.2021.1884802

Lucia Micheli, Giulia Collodel, Elena Moretti, Daria Noto, Andrea Menchiari, Daniela Cerretani, Sergio Crispino, Cinzia Signorini

{"title":"Redox imbalance induced by docetaxel in the neuroblastoma SH-SY5Y cells: a study of docetaxel-induced neuronal damage.","authors":"Lucia Micheli, Giulia Collodel, Elena Moretti, Daria Noto, Andrea Menchiari, Daniela Cerretani, Sergio Crispino, Cinzia Signorini","doi":"10.1080/13510002.2021.1884802","DOIUrl":"https://doi.org/10.1080/13510002.2021.1884802","url":null,"abstract":"Objectives: In cancer survivors, chemotherapy-associated adverse neurological effects are described as side effects in non-targeted tissue. We investigated the role of redox-imbalance in neuronal damage by a relative low dose of Docetaxel (DTX).Methods: The neuroblastoma cells (SH-SY5Y cells) were exposed to DTX at a dose of 1.25 nM for 6 h. Antioxidant defenses (i.e. ascorbic acid, glutathione, and catalase) and lipid oxidation products (i.e. F2-isoprostanes) were evaluated. To investigate cell ultrastructure and tubulin localisation, transmission electron microscopy (TEM) and immunofluorescence techniques were applied.Results: In the SH-SY5Y cells, DTX induced a significant reduction of total glutathione (P < 0.001) and ascorbic acid (P < 0.05), and an increase in both total F2-Isoprostanes (P < 0.05) and catalase activity (P < 0.05), as compared to untreated cells. Additionally, TEM showed a significant increase in cells with apoptotic characteristics. Immunolocalisation of tubulin showed a compromised cytoskeletal organisation.Discussion: The investigated sublethal dose of DTX, to which non-targeted cells may be exposed throughout the duration of chemotherapy treatment, induces a redox imbalance resulting in a specific modulation of the antioxidant response. This study provides new insights into DTX-induced cellular mechanisms useful for evaluating whether the concomitant use of antioxidants associated with chemotherapy mitigates chemotherapy side effects in cancer survivors.","PeriodicalId":21096,"journal":{"name":"Redox Report","volume":"26 1","pages":"18-28"},"PeriodicalIF":3.8,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/13510002.2021.1884802","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25350361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Comparison of the protective effect of cytosolic and mitochondrial Peroxiredoxin 5 against glutamate-induced neuronal cell death. 胞质过氧化氧还蛋白5和线粒体过氧化氧还蛋白5对谷氨酸诱导的神经元细胞死亡的保护作用比较。

IF 3.8 2区生物学

Redox Report Pub Date : 2021-12-01 DOI: 10.1080/13510002.2021.1901028

Mi Hye Kim, Da Yeon Kim, Hong Jun Lee, Young-Ho Park, Jae-Won Huh, Dong-Seok Lee

{"title":"Comparison of the protective effect of cytosolic and mitochondrial Peroxiredoxin 5 against glutamate-induced neuronal cell death.","authors":"Mi Hye Kim, Da Yeon Kim, Hong Jun Lee, Young-Ho Park, Jae-Won Huh, Dong-Seok Lee","doi":"10.1080/13510002.2021.1901028","DOIUrl":"https://doi.org/10.1080/13510002.2021.1901028","url":null,"abstract":"Objectives: Although glutamate is an essential factor in the neuronal system, excess glutamate can produce excitotoxicity. We previously reported that Peroxiredoxin 5 (Prx5) protects neuronal cells from glutamate toxicity via its antioxidant effects. However, it is unclear whether cytosolic or mitochondrial Prx5 provides greater neuroprotection. Here, we investigated differences in the neuroprotective effects of cytosolic and mitochondrial Prx5.Methods: We analyzed patterns of cytosolic and mitochondrial H2O2 generation in glutamate toxicity using HyPer protein. And then, we confirmed the change of intracellular ROS level and apoptosis with respective methods. The mitochondrial dynamics was assessed with confocal microscope imaging and western blotting.Results: We found that the level of mitochondrial H2O2 greatly increased compared to cytosolic H2O2 and it affected cytosolic H2O2 generation after glutamate treatment. In addition, we confirmed that mitochondrial Prx5 provides more effective neuroprotection than cytosolic Prx5.Discussion: Overall, our study reveals the mechanisms of cytosolic and mitochondrial ROS in glutamate toxicity. Our findings suggest that mitochondrial ROS and Prx5 are attractive therapeutic targets and that controlling these factors be useful for the prevention of neurodegenerative diseases.","PeriodicalId":21096,"journal":{"name":"Redox Report","volume":"26 1","pages":"53-61"},"PeriodicalIF":3.8,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/13510002.2021.1901028","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25479038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Possible relationship between mitochondrial changes and oxidative stress under low dose-rate irradiation. 低剂量率辐照下线粒体变化与氧化应激之间的可能关系

IF 5.2 2区生物学

Redox Report Pub Date : 2021-12-01 DOI: 10.1080/13510002.2021.1971363

Qingmei Meng, Elena Karamfilova Zaharieva, Megumi Sasatani, Junya Kobayashi

{"title":"Possible relationship between mitochondrial changes and oxidative stress under low dose-rate irradiation.","authors":"Qingmei Meng, Elena Karamfilova Zaharieva, Megumi Sasatani, Junya Kobayashi","doi":"10.1080/13510002.2021.1971363","DOIUrl":"10.1080/13510002.2021.1971363","url":null,"abstract":"Objectives: High dose-rate ionizing radiation (IR) causes severe DSB damage, as well as reactive oxygen species (ROS) accumulation and oxidative stress. However, it is unknown what biological processes are affected by low dose-rate IR; therefore, the molecular relationships between mitochondria changes and oxidative stress in human normal cells was investigated after low dose-rate IR.Methods: We compared several cellular response between high and low dose-rate irradiation using cell survival assay, ROS/RNS assay, immunofluorescence and western blot analysis.Results: Reduced DSB damage and increased levels of ROS, with subsequent oxidative stress responses, were observed in normal cells after low dose-rate IR. Low dose-rate IR caused several mitochondrial changes, including morphology mass, and mitochondrial membrane potential, suggesting that mitochondrial damage was caused. Although damaged mitochondria were removed by mitophagy to stop ROS leakage, the mitophagy-regulatory factor, PINK1, was reduced following low dose-rate IR. Although mitochondrial dynamics (fission/fusion events) are important for the proper mitophagy process, some mitochondrial fusion factors decreased following low dose-rate IR.Discussion: The dysfunction of mitophagy pathway under low dose-rate IR increased ROS and the subsequent activation of the oxidative stress response.","PeriodicalId":21096,"journal":{"name":"Redox Report","volume":"26 1","pages":"160-169"},"PeriodicalIF":5.2,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8405122/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39345222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum of limb remote ischemic postconditioning inhibits fMLP-triggered activation and reactive oxygen species releasing of rat neutrophils. 肢体远端缺血后适应血清抑制fmlp触发的大鼠中性粒细胞激活和活性氧释放。

IF 3.8 2区生物学

Redox Report Pub Date : 2021-12-01 DOI: 10.1080/13510002.2021.1982515

Gangling Chen, Jiangwei Zhang, Mingyue Sheng, Sanli Zhang, Qi Wu, Lei Liu, Boyang Yu, Junping Kou

{"title":"Serum of limb remote ischemic postconditioning inhibits fMLP-triggered activation and reactive oxygen species releasing of rat neutrophils.","authors":"Gangling Chen, Jiangwei Zhang, Mingyue Sheng, Sanli Zhang, Qi Wu, Lei Liu, Boyang Yu, Junping Kou","doi":"10.1080/13510002.2021.1982515","DOIUrl":"https://doi.org/10.1080/13510002.2021.1982515","url":null,"abstract":"Objectives: The study explores the protective role of the peripheral serum of limb remote ischemic postconditioning (LRIP) in reducing the reactive oxygen species (ROS) levels and neutrophil activation, which are responsible for the deleterious reperfusion injury.Methods: LRIP was induced in Sprague-Dawley rats by three cycles of 5 min occlusion /5 min reperfusion on the left hind limb. The blood samples were collected before LRIP or 0 and 1 h after LRIP (named SerumSham, SerumLRIP0, SerumLRIP1, respectively). The effects of LRIP serum on ROS level and neutrophils activation were determined. The expression of MyD88-TRAF6-MAPKs and PI3K/AKT pathways in neutrophils were examined.Results: When compared with SerumSham, SerumLRIP0 and SerumLRIP1 significantly reduced the ROS released from neutrophils activated by fMLP. Meanwhile, the mRNA expression levels of NADPH oxidase subunit p22phox and multiple ROS-producing related key proteins, such as NADPH oxidase subunit p47phox ser 304, ser 345. MyD88, p-ERK, p-JNK and p-P38 expression of neutrophils were downregulated by SerumLRIP0 and SerumLRIP1. SerumLRIP1 also downregulated p47phox mRNA expression and tumor necrosis factor receptor-associated factor 6 (TRAF6) protein expression.Conclusion: LRIP serum protects against ROS level and neutrophils activation involving the MyD88-TRAF6-MAPKs. This finding provides new insight into the understanding of LRIP mechanisms.","PeriodicalId":21096,"journal":{"name":"Redox Report","volume":"26 1","pages":"176-183"},"PeriodicalIF":3.8,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f2/28/YRER_26_1982515.PMC8530488.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39529022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Zingerone (4-(four-hydroxy-3-methylphenyl) butane-two-1) modulates adjuvant-induced rheumatoid arthritis by regulating inflammatory cytokines and antioxidants. 姜酮(4-(4-羟基-3-甲基苯基)丁烷- 2 -1)通过调节炎症细胞因子和抗氧化剂调节佐剂诱导的类风湿性关节炎。

IF 3.8 2区生物学

Redox Report Pub Date : 2021-12-01 DOI: 10.1080/13510002.2021.1907518

Nazirah Bashir, Sheikh Bilal Ahmad, Muneeb U Rehman, Showkeen Muzamil, Rahil Razak Bhat, Manzoor Ur Rahman Mir, Gamal A Shazly, Mohamed A Ibrahim, Gehan M Elossaily, Abdelrahman Y Sherif, Mohsin Kazi

{"title":"Zingerone (4-(four-hydroxy-3-methylphenyl) butane-two-1) modulates adjuvant-induced rheumatoid arthritis by regulating inflammatory cytokines and antioxidants.","authors":"Nazirah Bashir, Sheikh Bilal Ahmad, Muneeb U Rehman, Showkeen Muzamil, Rahil Razak Bhat, Manzoor Ur Rahman Mir, Gamal A Shazly, Mohamed A Ibrahim, Gehan M Elossaily, Abdelrahman Y Sherif, Mohsin Kazi","doi":"10.1080/13510002.2021.1907518","DOIUrl":"10.1080/13510002.2021.1907518","url":null,"abstract":"Objective: Ginger (Zingiber officinale Roscoe) is considered to be one of the most commonly consumed dietary condiments of the world. The present study was designed to explicate the protective role of zingerone; an active ingredient of ginger in complete Freund's adjuvant (FCA)-immunized arthritic rats.Methods: 24 Wistar rats were divided into 4 groups with 6 rats each. Group I as control followed by group II, III and IV were treated with single intradermal injection of FCA (0.1 ml = 100 µg) to induce rheumatoid arthritis. Group III and IV were also administered with zingerone orally at 25 mg/kg b.w for 3 weeks at two different time points.Results: Adjuvant-treated rats exhibited a significant increase in lipid peroxidation and a reduction in the enzymatic antioxidants such as SOD, catalase and GPx, in the liver and joint tissues. Moreover, FCA inoculation resulted in the increase in levels of NF-κB, TGF-β, TNF-α, IL-1β, IL-6 and Hs-CRP and a decrease in IL-10 levels. Zingerone significantly reduced the levels of NF-κB, TGF-β, TNF-α, IL-1β, IL-6 and Hs-CRP and markedly increased IL-10 levels. Levels of antioxidant enzymes were also restored by zingerone treatment.Discussion: Oral administration of zingerone ameliorated inflammatory outburst and decreased oxidative stress, suggesting its role in the prevention of rheumatoid arthritis. Further mechanistic insights are necessary to study the exact mechanism involved.","PeriodicalId":21096,"journal":{"name":"Redox Report","volume":"26 1","pages":"62-70"},"PeriodicalIF":3.8,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/13510002.2021.1907518","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25533504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16