Process Biochemistry最新文献

{"title":"Photocatalytic dye detoxification by cost-effective SnO2: Ce thin films and verification of toxicity by histology using Labeo rohita fish","authors":"K. Ravichandran ,&nbsp;K. Gayathri ,&nbsp;M. Baneto ,&nbsp;B. Kaleeswaran ,&nbsp;P.K. Praseetha ,&nbsp;B. Deepa ,&nbsp;N. Anuradha","doi":"10.1016/j.procbio.2025.05.014","DOIUrl":"10.1016/j.procbio.2025.05.014","url":null,"abstract":"<div><div>This study is aimed to provide detailed insights into the photocatalytic degradation ability of cerium-doped tin oxide (SnO₂: Ce) thin films fabricated using a cost-effective nebulizer spray pyrolysis technique. Characterization of the deposited samples was performed using XRD, SEM, EDS, E-mapping, XPS, PL and UV and the obtained results were correlated with the photocatalytic dye detoxification results. The effect of cerium doping at different concentrations (2.5, 5.0 and 7.5 wt%) on the ability of the films to break down methylene blue (MB), a toxic dye commonly found in industrial effluents was studied. The toxicity of the water treated with the doped films was evaluated by exposing freshwater fish <em>Labeo rohita</em> to the treated water and performing the histology study of different organs of the test fish using the paraffin wax method. The results were compared with the toxicity observed in the fish exposed to untreated methylene blue dye solution. The photocatalytic studies revealed a significant enhancement in the photocatalytic dye degrading efficiency of SnO₂ with the addition of Ce. The SnO₂: Ce (5 %) film exhibited 96.44 % decomposition of Methylene Blue (MB) dye molecules.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 118-135"},"PeriodicalIF":3.7,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144178795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fiber composite hydrogels: Advances in food preservation and biomedical applications","authors":"Xiaowei Xu ,&nbsp;Qian Ning ,&nbsp;Hanbin Chen ,&nbsp;Jie Pang","doi":"10.1016/j.procbio.2025.05.019","DOIUrl":"10.1016/j.procbio.2025.05.019","url":null,"abstract":"<div><div>This review synthesizes the recent advances in fiber composite hydrogels, systematically analyzing their structural design, fabrication methods, and applications in biomedical and food science domains. By integrating 100 studies, we highlight structural characteristics and advanced fabrication techniques such as microfluidic spinning, microfluidic blow-spinning and 3D bioprinting. In biomedical fields, quantitative data highlight their efficacy in engineering scaffolds, wound healing, and drug delivery. Current research on fiber composite hydrogels in food applications remains underdeveloped, with predominant focus areas confined to stimuli-responsive nutrient delivery systems and active packaging preservation mechanisms. With the accelerated advancement in intelligent materials research, substantial innovation prospects are emerging for AI-driven flexible material design and sustainable biomass utilization. Fiber composite hydrogels have important implications for translating laboratory innovations into practical solutions.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 164-174"},"PeriodicalIF":3.7,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144203958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SpyTag/SpyCatcher-mediated SpyRing cyclization improves the operational stability and renaturation efficiency of immobilized enzymes after catalytic inactivation","authors":"Yao Ma ,&nbsp;Jincheng Miao ,&nbsp;Qiwei Chen ,&nbsp;Hongxu Sun ,&nbsp;Yanhong Chang ,&nbsp;Hui Luo","doi":"10.1016/j.procbio.2025.05.017","DOIUrl":"10.1016/j.procbio.2025.05.017","url":null,"abstract":"<div><div>L-Phenylserine aldolase (LPA), a promising industrial multi-subunit enzyme, catalyzes the condensation of aldehydes with glycine to form β-hydroxy amino acids. This study investigated the effect of SpyTag/SpyCatcher-mediated SpyRing cyclization on operational stability and renaturation after catalytic inactivation. Two immobilized forms of LPA were prepared on a glyoxyl-agarose support (Gx): the native Gx-LPA and its SpyRing-cyclized variant, Gx-SRLPA. The immobilized cyclized enzyme, Gx-SRLPA, exhibited superior operational stability compared to Gx-LPA. A renaturation strategy involving partial unfolding with low concentrations of guanidine hydrochloride (GuHCl) followed by renaturation, proved effective. Gx-SRLPA recovered up to 87.9 % of its activity at 1 M GuHCl and pH 9, exceeding Gx-LPA’s 52.9 % recovery at 1 M GuHCl and pH 7. The presence of dithiothreitol in the unfolding solution and weak alkaline conditions further enhanced Gx-SRLPA’s renaturation. This study highlights the stabilizing effect of the SpyRing structure on enzymes and provides valuable insights into the renaturation of immobilized multi-subunit proteins.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 93-103"},"PeriodicalIF":3.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144166434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient degradation of azo dyes by an engineered myoglobin with a modified heme active site showing high peroxidase activity","authors":"Jing-Jing Liu ,&nbsp;Bin-Bin Jiang ,&nbsp;Ya-Han Zhu ,&nbsp;Shu-Qin Gao ,&nbsp;Ying-Wu Lin","doi":"10.1016/j.procbio.2025.05.018","DOIUrl":"10.1016/j.procbio.2025.05.018","url":null,"abstract":"<div><div>Azo dyes, widely used in global textiles, persist in wastewater causing significant ecological and health risks, necessitating efficient degradation to protect ecosystems and public health. The development of artificial metalloenzymes has provided advanced biotechnological solutions to address this challenge. This study presents a highly efficient artificial metalloenzyme, F43Y/H64D myoglobin (F43Y/H64D Mb), engineered from sperm whale myoglobin with a modified heme active site by replacement of Phe43 with Tyr and His64 with Asp, respectively, for azo dye degradation. The engineered enzyme achieved degradation efficiencies of approximately 93 %, 93 %, and 90 % for Acid Orange 7, Methyl Orange, and Acid Blue 92, respectively, within 10 seconds. Its overall catalytic efficiency was found to be approximately 3.6 times higher than that of horseradish peroxidase, a widely used natural enzyme. HPLC and ESI-MS analyses proposed degradation pathways, and bacterial toxicity tests confirmed reduced toxicity post-degradation. Furthermore, F43Y/H64D Mb exhibited excellent performance in natural aquatic environments, surpassing many recently reported results in efficiency, reaction speed, and operational simplicity. These findings highlight the feasibility of F43Y/H64D Mb for efficient azo dye decolorization and degradation in wastewater, offering a novel enzymatic tool for sustainable environmental remediation.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 153-163"},"PeriodicalIF":3.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144194426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Platelets as circulatory drug carriers: Emerging potential and its advantages over conventional delivery systems","authors":"Farzana Naushin ,&nbsp;Aryesh Acharjee ,&nbsp;Antik Chakraborty ,&nbsp;Md Tarique Waheed ,&nbsp;Jaydeep Bhattacharya","doi":"10.1016/j.procbio.2025.05.016","DOIUrl":"10.1016/j.procbio.2025.05.016","url":null,"abstract":"<div><div>Traditional systemic drug delivery methods are often limited by faster clearance rates and high dose requirements. While innovative drug delivery systems, such as liposomes, polymersomes, and solid lipid nanoparticles etc., offer the ability to carry hydrophilic and hydrophobic drugs. But the lack of specificity in the current delivery systems leads to reduced therapeutic efficacy. To overcome these, functionalization with small molecules like peptides, aptamers, and antibodies is done. But they introduce new immunogenicity, stability, and complexity challenges, making these methods costly and less practical. To address these issues, circulatory cell-based drug delivery systems have gained attention as a promising alternative. Unlike red blood cells (RBCs), which have the Rh factor that can complicate, platelets are devoid of this factor and possess inherent advantages. Their natural properties, including non-immunogenicity, hemostasis, pathogen interaction, tissue regeneration, and wound healing, make them highly effective for targeted drug delivery. Furthermore, platelets can form leaky junctions at tumor sites, enhancing localized drug delivery and improving therapeutic outcomes. Thus, platelet-based drug delivery systems are gaining popularity. Moreover, the lower production costs make platelets an efficient and promising tool for a smart drug delivery system. This review highlights the significant role of platelets in advancing targeted drug delivery and their potential to revolutionize therapeutic strategies.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 136-152"},"PeriodicalIF":3.7,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144185011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The zero-valent iron mediated anaerobic phosphate reduction system drives the recovery of dual products from phosphorus-enriched sludge","authors":"Zhixuan Fan ,&nbsp;Bing Wang ,&nbsp;Yunlong Liu ,&nbsp;Jiyuan Li","doi":"10.1016/j.procbio.2025.05.015","DOIUrl":"10.1016/j.procbio.2025.05.015","url":null,"abstract":"<div><div>This study developed a zero-valent iron (ZVI) mediated anaerobic phosphate reduction system that innovatively recovers resources from phosphorus-rich sludge by coupling phosphine (PH₃) generation with vivianite crystallization. The system overcomes the low recovery efficiency and limited product diversity of traditional methods by enabling multiphase transformation of elemental phosphorus. This was achieved by optimizing the ZVI-driven carbon and phosphorus metabolic network (influent COD 3000 mg/L, TP 50 mg/L). Cumulative PH₃ production reached 46.15 mg/L, vivianite yield was 810 mg, and the iron utilization rate increased to 58 %. Microbiome analysis revealed multi-level regulatory effects of ZVI on microbial community. At the phylum level, <em>Bacteroidetes</em> (+8.1 %) promoted ZVI corrosion; <em>Spirochaetes</em> (+7.44 %) facilitated electron transfer network formation; <em>Caldiserica</em> (+9.3 %) and <em>Actinobacteria</em> (+2.7 %) synergistically catalyze phosphate reduction and enhance PO₄³⁻ availability. At the genus level, <em>Atopobium</em> (+7 %) promoted organophosphorus mineralization; <em>Caldisericum</em> (+8 %) and <em>Prevotellaceae</em> (+8.67 %) activated phosphorus-reducing bacteria via H₂ oxidation and fatty acid metabolism; <em>Megasphaera</em> (+4.33 %) regulated pH to optimize Fe²⁺–PO₄^3- co-precipitation, creating a “phosphorus release-reduction-fixation” microenvironment. Thermodynamic analysis confirmed that the strong reducing capacity of ZVI drives the coupled pathways of vivianite precipitation (Δ_rG_m^θ = −397.78 kJ/mol) and PH₃ biosynthesis (Δ_rG_m^θ = −110.82 kJ/mol). Through chemical–biological synergy, this study proposes a novel strategy for the efficient and sustainable utilization of phosphorus-rich sludge.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 104-117"},"PeriodicalIF":3.7,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144166435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of pasteurization and thermosonication on phytocompounds in Titay juice: Predictive modelling with ANN-GA and extraction kinetics","authors":"Puja Das ,&nbsp;Prakash Kumar Nayak ,&nbsp;Radha krishnan Kesavan","doi":"10.1016/j.procbio.2025.05.011","DOIUrl":"10.1016/j.procbio.2025.05.011","url":null,"abstract":"<div><div>This study aimed to enhance the extraction of phytocompounds from Titay (<em>Phlogacanthus thyrsiformis</em>) vegetable juice through the application of thermosonication (TS). An artificial neural network (ANN) combined with a genetic algorithm (GA) was utilized to model and optimize the process. The effects of ultrasonic amplitude (30–50 %), temperature (30–50°C), and sonication duration (15–60 minutes) on total polyphenolic content (TPC), total flavonoid content (TFC), antioxidant activity (AOA), ascorbic acid content (AA), total anthocyanin content (TAC), and total chlorophyll content (TCH) were investigated. Optimal TS conditions were identified as 40 % amplitude, 40°C temperature, and 60 minutes sonication time. Following this, an investigation into the extraction kinetics and thermodynamics of phytochemical compounds from TJ using the TS process was conducted across various combinations of temperature (30–50°C) and extraction time (0–60 mins). Extraction kinetics demonstrated R² (0.98354) and χ² (0.2992) for TPC with E_a 73.26, R² (0.99023) and χ² (0.1387) for TFC with E_a 71.46, R² (0.98842) and χ² (0.0076) for AOA with E_a 46.77, R² (0.98250) and χ² (0.0394) for AA with E_a 97.97, R² (0.99441) and χ² (0.0051) for TAC with E_a 84.98, R² (0.98449) and χ² (0.0238) for TCH with E_a 158.55 using pseudo second order (PSO) kinetic model. The experimental results demonstrated a consistent trend across all six responses: as the temperature rose, both the maximum concentration of the phytochemical compound achievable in the liquid extract and the speed of the extraction process increased. Specifically, the saturation concentration (S_C) and the rate constant (k) for the PSO kinetic model showed positive correlations with temperature. These findings provide valuable insights into the extraction dynamics, enabling researchers to forecast extraction efficiency and determine optimal parameters for scaling up the process to industrial levels.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 76-92"},"PeriodicalIF":3.7,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144146837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metaproteomics of human microbiota: Progress and key challenges for precision medicine","authors":"Feng Yu ,&nbsp;Dongbo Liu ,&nbsp;Yongheng Chen ,&nbsp;Wei Zhang ,&nbsp;Yijing He","doi":"10.1016/j.procbio.2025.05.013","DOIUrl":"10.1016/j.procbio.2025.05.013","url":null,"abstract":"<div><div>Human metaproteomics, the large-scale study of proteins from microbiota in different parts of human body, has gained significant attention over the past few decades due to its potential to provide comprehensive insights into microbial communities and their functions. In terms of experimental techniques, continuous advancements in mass spectrometry and bioinformatics have propelled the development of metaproteomics. The emergence of artificial intelligence enables more specific identification and quantification of proteins from complex microbial ecosystems. Additionally, in the realm of clinical applications, metaproteomics offers critical contributions to disease diagnosis and treatment by elucidating the taxonomic and functional dynamics within the human microbiota. However, the complexity of metaproteomics data requires high sensitivity, robust bioinformatics pipelines and large-scale, systematic clinical validation, which restrict the widespread adoption of metaproteomics in routine clinical settings. In this review, we summarize recent advancements in metaproteomics, highlight novel technologies, and discuss their practical applications in multiple diseases, such as cancer and COVID-19. We also provide an overview of the challenges and future directions in metaproteomics, emphasizing its role in advancing our understanding of microbiota.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 59-75"},"PeriodicalIF":3.7,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144138382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound-assisted intensified removal of dextran from sugarcane juice using dextranase","authors":"Priya Yadav,&nbsp;Viraj N. Khasgiwale,&nbsp;Parag R. Gogate","doi":"10.1016/j.procbio.2025.05.012","DOIUrl":"10.1016/j.procbio.2025.05.012","url":null,"abstract":"<div><div>The present work demonstrates the effectiveness of dextranase-based ultrasound-assisted enzymatic treatment in reducing dextran content in sugarcane juice. Effect of various parameters such as enzyme loading (0.01 U – 0.07 U) treatment duration (5–20 min), temperature (40–70°C), pH (4−7), ultrasonic power (20–80 W), and duty cycle (20–50 %) on the dextran removal was studied. The scalability and efficiency of several ultrasonic reactors (US bath, hexagonal, and horn reactors) including the variation of frequencies (22, 33, and 48 kHz) using hexagonal reactor were also examined. Ultrasound-assisted treatment at 50°C, pH 5, a 10-min time, 30 % duty cycle, and 40 W power produced the best results for the highest dextran elimination (93 %). By contrast, only 63 % of the dextran was removed using the traditional method. Ultrasonic horn demonstrated the highest efficiency (93 %) attributed to higher power density followed by hexagonal reactor and ultrasonic baths (81 % and 72 %, respectively). Furthermore, the best results for dextran removal were obtained with single-frequency ultrasound at 22 kHz; whereas higher and combination frequencies resulted in decreased effectiveness because of wave interference and denaturation of the enzyme. The work has clearly demonstrated the effectiveness of ultrasound also elucidating the effect of reactor type and frequency for the first time.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 47-58"},"PeriodicalIF":3.7,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144134059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of the interaction between the de novo biosynthesis of vitamin B6 and its salvage pathway for efficient production of pyridoxine","authors":"Mengqing Lu,&nbsp;Haoran Sun,&nbsp;Zanwen Chen,&nbsp;Naiqiang Li","doi":"10.1016/j.procbio.2025.05.010","DOIUrl":"10.1016/j.procbio.2025.05.010","url":null,"abstract":"<div><div>Vitamin B6, a micronutrient essential for maintaining healthy growth and normal physiological function, is a generic term for six interconvertible substances, of which pyridoxine hydrochloride is the most common commercial form, produced industrially by chemical processes using expensive and/or toxic chemicals. To date, attempts to engineer microorganisms to produce high-level of vitamin B6 have been unsuccessful due to the toxic intermediates and a lack of understanding of the vitamin B6 metabolism regulation. Here, for the first time, we proposed a two-step process for efficient pyridoxine production. A recombinant <em>Escherichia coli</em> was successfully engineered to produce 1406 mg/L of pyridoxal phosphate by interfering with only one or two key enzyme genes expression in the vitamin B6 salvage pathway. Pyridoxal phosphate was then efficiently converted to pyridoxine by another engineered <em>Escherichia coli</em> overexpressing cell membrane-anchored phosphatase YbhA and pyridoxal reductase PdxI, and 2296 mg/L (calculated as pyridoxine hydrochloride) of pyridoxine was achieved with a yield of 0.29 mol/mol (pyridoxine hydrochloride/pyridoxal phosphate) by shake flask cultivation. This achievement marked a significant advance in the biological synthesis of vitamin B6 and demonstrated an efficient vitamin B6 production strategy by reducing interference between the de novo synthesis pathway and the salvage pathway of vitamin B6.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 1-7"},"PeriodicalIF":3.7,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144105629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}