Process Biochemistry最新文献_第5页

Novel extraction and purification technologies for anthraquinones: Towards sustainable food preservation solutions

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-01-27 DOI: 10.1016/j.procbio.2025.01.020

Juanjuan Yang , Zerong Pei , Hongyi Yang , Wenwen Deng , Hui Li

{"title":"Novel extraction and purification technologies for anthraquinones: Towards sustainable food preservation solutions","authors":"Juanjuan Yang , Zerong Pei , Hongyi Yang , Wenwen Deng , Hui Li","doi":"10.1016/j.procbio.2025.01.020","DOIUrl":"10.1016/j.procbio.2025.01.020","url":null,"abstract":"<div><div>Food preservation technologies are pivotal for extending the shelf life of food products by incorporating chemical preservatives, thus ensuring the maintenance of flavor, nutritional content, and safety. As consumer demand for food safety and quality continues to escalate, there has been a burgeoning interest in natural alternatives due to their gentle antimicrobial properties and enhanced safety profiles. Among these, anthraquinones, predominantly derived from botanical sources such as rhubarb, senna, aloe vera, knotweed, and madder, have garnered significant attention. Recent findings have underscored the exceptional antimicrobial and antioxidant properties of anthraquinones, positioning them as promising candidates for natural food preservatives with substantial developmental and application prospects within the food preservation sector. Therefore, there has been a surge in the development of innovative extraction and purification methodologies aimed at harnessing natural anthraquinones from their botanical reservoirs. This review summarizes the current state of research on extraction and purification techniques for anthraquinones from a variety of natural plant species, along with an evaluation of their antimicrobial and antioxidant efficacies in the context of food preservation. Additionally, it delves into the preservative properties of anthraquinone compounds to offer insights that may facilitate efficient production and utilization of natural anthraquinones for food preservation.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"150 ","pages":"Pages 328-341"},"PeriodicalIF":3.7,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143099625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extraction, purification, component analysis and bioactivity of polyphenols from wampee

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-01-27 DOI: 10.1016/j.procbio.2025.01.025

Yuan-yuan Ren, Mosa Makhele, Jue-jun Zhou, Peng-peng Sun

{"title":"Extraction, purification, component analysis and bioactivity of polyphenols from wampee","authors":"Yuan-yuan Ren, Mosa Makhele, Jue-jun Zhou, Peng-peng Sun","doi":"10.1016/j.procbio.2025.01.025","DOIUrl":"10.1016/j.procbio.2025.01.025","url":null,"abstract":"<div><div>Wampee, a kind of medicinal and edible fruit, contains abundant bioactive compounds. In order to realize comprehensive utilization of wampee by-products, the extraction parameters for wampee polyphenols were optimized using single-factor and response surface tests, followed by the purification with AB-8 macroporous resin. Then component analysis and bioactivity evaluation in vitro were conducted. Result indicated the optimal extraction parameters of wampee polyphenols were: ethanol concentration 56 %, extraction temperature 62℃, material-liquid ratio being 1:19 g/mL, and extraction time 1 h. AB-8 resin exhibited high adsorption rate (91.42 % ± 0.18 %) and desorption rate (89.60 % ± 0.90 %), leading to increased purity of wampee polyphenols (WPP) from 12.51 % ± 0.36–53.8 % ± 1.87 %. UV and FTIR spectra confirmed the presence of polyphenolic compounds in WPP, including unique phenolic Vc-O and primary alcohol structures. LC-MS analysis identified six distinct polyphenolic compounds in WPP. In addition, the bioactivity of WPP enhanced, especially hydroxyl radical scavenging ability. Our research would provide guidance for the comprehensive utilization of wampee resources and its application in functional foods.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"150 ","pages":"Pages 318-327"},"PeriodicalIF":3.7,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143099609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transforming dairy effluent into valuable resources: Harnessing microalgae for sustainable production of nutraceuticals and pharmaceuticals

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-01-27 DOI: 10.1016/j.procbio.2025.01.026

Udaypal, Rahul Kumar Goswami, Pradeep Verma

{"title":"Transforming dairy effluent into valuable resources: Harnessing microalgae for sustainable production of nutraceuticals and pharmaceuticals","authors":"Udaypal, Rahul Kumar Goswami, Pradeep Verma","doi":"10.1016/j.procbio.2025.01.026","DOIUrl":"10.1016/j.procbio.2025.01.026","url":null,"abstract":"<div><div>The dairy industry generates nutrient-rich effluent, significantly contributing to global eutrophication and waterborne diseases. Dairy effluent (DE) can be transformed from waste into valuable products by using it as a cultivation medium for microalgae. Microalgae have high biomass productivity and are rich in lipids, proteins, carbohydrates, vitamins, and carotenoids. These compounds are in high demand in the nutraceutical and pharmaceutical industries. This review examines DE as a sustainable alternative to synthetic media for cultivating microalgae, which is rich in nutrients like nitrogen, phosphorus, and lactose. It compares the composition of processed dairy effluent (PDE) with synthetic media, showcasing its potential for microalgae cultivation for nutraceutical and pharmaceutical applications. The review also highlights the environmental risks of untreated DE and advocates for PDE-based cultivation as an eco-friendly solution. It discusses photobioreactor (PBR) designs optimized for PDE treatment and explores the global market for microalgal products. Challenges such as scaling production and regulatory hurdles have been discussed, emphasizing the need for sustainable microalgae cultivation. This review advances algal biorefinery research by presenting DE as a sustainable cultivation medium for producing high-value microalgal products using suggested PBRs. It highlights strategies to integrate wastewater treatment with bioproduct generation, fostering scalable and eco-friendly solutions.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"150 ","pages":"Pages 342-356"},"PeriodicalIF":3.7,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143156045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing polymeric nano-composite ceramic membrane performance and sustainable recovery for palm oil mill effluent (POME) wastewater treatment using advanced chemometric algorithms

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-01-26 DOI: 10.1016/j.procbio.2025.01.022

Jamilu Usman , Yusuf Olabode Raji , Sani. I. Abba , A.G. Usman , Lukka Thuyavan Yogarathinam , Fahad Jibrin Abdu , Mohd Hafiz Dzarfan Othman , Isam H. Aljundi

{"title":"Enhancing polymeric nano-composite ceramic membrane performance and sustainable recovery for palm oil mill effluent (POME) wastewater treatment using advanced chemometric algorithms","authors":"Jamilu Usman , Yusuf Olabode Raji , Sani. I. Abba , A.G. Usman , Lukka Thuyavan Yogarathinam , Fahad Jibrin Abdu , Mohd Hafiz Dzarfan Othman , Isam H. Aljundi","doi":"10.1016/j.procbio.2025.01.022","DOIUrl":"10.1016/j.procbio.2025.01.022","url":null,"abstract":"<div><div>This study investigates the enhancement of emulsified oily wastewater treatment using high-performance poly (diallyldimethylammonium chloride) PDADMAC ultrafiltration membranes through a multi-model machine learning (ML) approach. The study was based on experimental scenarios and more emphasis on computational learning applications. In this context, kernel Gaussian Process Regression (GPR), Linear Regression (LR), Stepwise Regression (SWR), and Multiple Linear Regression (MLR) were employed to predict water flux (WF) and oil rejection (OR). Subsequently, traditional Response Surface Methodology (RSM) was developed for predictive comparison. The predictive skills were evaluated and visualized using statistical indicators and 2-dimensional diagrams. GPR achieved the highest predictive accuracy for OR, with an NSE of 99.32 %, zero bias (PBIAS 0.0000), and the lowest MAE (0.0010). For WF, the RSM-W) model outperformed others with an NSE of 82.03 %, the lowest MAE (0.0051), and a slight underestimation bias (PBIAS −0.0587). These models significantly outperformed RLR, SWR, and MLR, which showed moderate accuracy and higher prediction errors. The environmental implications align with the goals of the Environmental Protection Agency (EPA) and the United Nations Sustainable Development Goals (SDGs). Enhanced treatment processes contribute to cleaner water bodies, protect marine ecosystems, and promote sustainable industrial practices. Future research should focus on field trials to validate these models under real-world conditions, integration with real-time monitoring systems for dynamic adjustments, and life cycle assessments to evaluate long-term sustainability.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"150 ","pages":"Pages 306-317"},"PeriodicalIF":3.7,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143099602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosynthesis and modification strategies of novel cyclic lipopeptide secreted by Bacillus spp.: Research progress

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-01-26 DOI: 10.1016/j.procbio.2025.01.023

Zhengjun Pang , Wenshuo Zhang , Bo Zhang , Shiza Navaz , Fenghuan Wang , Yonghong Liao

引用次数: 0

Toward sustainable bioplastics: The potential of algal biomass in PHA production and biocomposites fabrication

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-01-24 DOI: 10.1016/j.procbio.2025.01.019

Raul E. Martínez-Herrera , Georgia M. González-Meza , Edgar R. Meléndez-Sánchez

{"title":"Toward sustainable bioplastics: The potential of algal biomass in PHA production and biocomposites fabrication","authors":"Raul E. Martínez-Herrera , Georgia M. González-Meza , Edgar R. Meléndez-Sánchez","doi":"10.1016/j.procbio.2025.01.019","DOIUrl":"10.1016/j.procbio.2025.01.019","url":null,"abstract":"<div><div>This review hypothesizes that algal biomass, an underexplored and promising raw material, offers significant potential for polyhydroxyalkanoate (PHA) production within sustainable bioprocess frameworks. The increasing urgency to find alternative, eco-friendly solutions to traditional plastic production is driven by growing environmental concerns over plastic pollution. The review highlights novel interdisciplinary connections between algal biomass utilization, bioremediation, and PHA biosynthesis, revealing new pathways that optimize renewable resource use and enhance waste valorization. These hybrid processes, combining microalgal and cyanobacterial biomass after bioactive compound extraction, have not been sufficiently explored, presenting significant environmental and economic opportunities for the bioplastics industry. Additionally, the integration of artificial intelligence (AI) in the development of PHA + algal biomass biocomposites, with desirable properties for industrial and medical applications, presents a breakthrough opportunity. Future research should focus on advancing pretreatment methods to improve fermentable carbohydrate availability, scaling up production, and addressing challenges related to energy efficiency, cost-effectiveness, and the commercial viability of these biocomposites in the bioplastics market.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"150 ","pages":"Pages 276-287"},"PeriodicalIF":3.7,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143099603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A review on biodesulfurization of crude oil using different microorganisms: Reaction mechanisms, effective factors, and removal efficiency of organic sulfur compounds

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-01-23 DOI: 10.1016/j.procbio.2025.01.018

Sajad Tamjidi , Hossein Esmaeili

{"title":"A review on biodesulfurization of crude oil using different microorganisms: Reaction mechanisms, effective factors, and removal efficiency of organic sulfur compounds","authors":"Sajad Tamjidi , Hossein Esmaeili","doi":"10.1016/j.procbio.2025.01.018","DOIUrl":"10.1016/j.procbio.2025.01.018","url":null,"abstract":"<div><div>Thiophene, benzothiophene and dibenzothiophene are the main organic sulfur compounds in crude oil, which reduce the quality of crude oil. Previous studies have shown that biodesulfurization (BDS) is the most efficient method for the separation of complex organic sulfur compounds from crude oil. BDS is performed at ambient pressure and temperature with excellent selectivity, leading to reduced energy cost, low emissions of CO<sub>2</sub> and lack of production of unfavorable by-products. In this review study, the advantages and disadvantages of BDS compared to other processes, the performance of various microorganisms in the removal of complex sulfur compounds, environmental impacts, and biodesulfurization kinetics were thoroughly discussed. Also, different mechanisms of biodesulfurization process and their pathways in removing sulfur compounds were investigated. Moreover, the utilization of genetic engineering to improve biodesulfurization efficiency was studied. According to previous studies, the Bintulu oil field in Malaysia and the Boscan oil field in Venezuela have the lowest (0.03 %) and the highest sulfur content (5.7 %), respectively. Also, some microorganisms such as <em>Mycobacterium goodie</em> X7B, <em>Rhodococcus erythropolis</em> XP, <em>Rhodococcus sp</em>. ECRD-1, and <em>Gordonia alkanivorans</em> have shown significant biodesulfurization efficiency. In general, the integration of BDS and hydrodesulfurization processes is recommended as an efficient and environmentally friendly process in removing complex sulfur compounds.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"150 ","pages":"Pages 288-305"},"PeriodicalIF":3.7,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143099612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxygen uptake rate (OUR) guided nitrogen control strategy for improving nemadectin production by Streptomyces cyaneogriseus ssp. noncyanogenus

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-01-20 DOI: 10.1016/j.procbio.2025.01.017

Xiaoqing Song , Zishu Zhang , Junxiong Yu , Yun Zhang , Jiayun Xue , Yuanxin Guo , Jianguang Liang , Min Ren , Qingyun Ling , Ali Mohsin , Jiangchao Qian , Zejian Wang , Yonghong Wang

{"title":"Oxygen uptake rate (OUR) guided nitrogen control strategy for improving nemadectin production by Streptomyces cyaneogriseus ssp. noncyanogenus","authors":"Xiaoqing Song , Zishu Zhang , Junxiong Yu , Yun Zhang , Jiayun Xue , Yuanxin Guo , Jianguang Liang , Min Ren , Qingyun Ling , Ali Mohsin , Jiangchao Qian , Zejian Wang , Yonghong Wang","doi":"10.1016/j.procbio.2025.01.017","DOIUrl":"10.1016/j.procbio.2025.01.017","url":null,"abstract":"<div><div>Oxygen supply and oxygen uptake rate (OUR) significantly influences both the physiological state of cells and nemadectin biosynthesis by <em>Streptomyces cyaneogriseus ssp. noncyanogenus.</em> In this study, a real-time monitoring approach for nemadectin fermentation was developed for the first time by an online OUR control strategy. The effect of OUR levels during the mycelium differentiation phase on nemadectin biosynthesis was studied and optimized by adjusting feeding rates of (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub>. Results showed that controlling the OUR at approximately 17.5 mmol·L<sup>−1</sup>·h<sup>−1</sup> during the mycelium differentiation phase effectively enhanced nemadectin production, reaching 2805.1 μg·mL<sup>−1</sup>, making a 129.5 % increase compared to the control. This OUR control strategy was successfully scaled-up to a 500 L pilot scale, achieving the highest ever nemadectin production of 2867.3 μg·mL<sup>−1</sup>. These findings demonstrate that this OUR control strategy provides an effective and scalable approach for industrial nemadectin production.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"150 ","pages":"Pages 248-256"},"PeriodicalIF":3.7,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143094486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mathematical prediction of COD removal using interrelation of operating variables of the aerobic inverse fluidized bed biofilm reactor (AIFBBR)

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-01-20 DOI: 10.1016/j.procbio.2025.01.014

Mallikarjuna Challa, Rajesh Roshan Dash

{"title":"Mathematical prediction of COD removal using interrelation of operating variables of the aerobic inverse fluidized bed biofilm reactor (AIFBBR)","authors":"Mallikarjuna Challa, Rajesh Roshan Dash","doi":"10.1016/j.procbio.2025.01.014","DOIUrl":"10.1016/j.procbio.2025.01.014","url":null,"abstract":"<div><div>The present study aimed to develop a mathematical model for predicting COD removal using the interrelation of operating variables. The model for predicting COD removal is named the dimensionless number for COD removal (DNC). The DNC was formulated based on the critical variables of the AIFBBR process for the rice mill wastewater treatment. HRT, OLR, and biomass concentration (M<sub>b</sub>) are considered for the generation of DNC for the AIFBBR system. In the present attempt of framing such a dimensionless number DNC, many experimental runs were conducted by varying the COD strength of 0.8–3.2 kg/m<sup>3</sup>, OLR of 0.80–6.40 kg/m<sup>3</sup>.d, and M<sub>b</sub> of 0.90–7.43 kg/m<sup>3</sup>. The developed DNC was subjected to sensitivity analysis, and biomass concentration in the system was observed to be the most sensitive among other parameters incorporated in the model. In addition, from the sensitivity analysis, it was also revealed that the removal efficiencies were observed to increase with the increase in DNC values in the COD range of 0.8–3.2 kg/m<sup>3</sup>. In addition, the plot between the reported removals from the literature on biofilm processes for wastewater treatment and DNC values yielded an <em>R</em><sup><em>2</em></sup> value of 0.79.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"150 ","pages":"Pages 257-264"},"PeriodicalIF":3.7,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143094489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RSM-based optimization and scale-up production of hIL-2 in Escherichia coli

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-01-20 DOI: 10.1016/j.procbio.2025.01.015

Yuxiao Liu, Mingfang Zhang, Yueying Xu, Lin Yang, Fei Wang, Yuming Fang, Jiayu Wang, Xiaoran Jing, Junjun Jiang

{"title":"RSM-based optimization and scale-up production of hIL-2 in Escherichia coli","authors":"Yuxiao Liu, Mingfang Zhang, Yueying Xu, Lin Yang, Fei Wang, Yuming Fang, Jiayu Wang, Xiaoran Jing, Junjun Jiang","doi":"10.1016/j.procbio.2025.01.015","DOIUrl":"10.1016/j.procbio.2025.01.015","url":null,"abstract":"<div><div>Human interleukin 2 (hIL-2) has become a pivotal molecule widely used in the treatment of various diseases. The development of a robust and highly productive high cell density cultivation process for producing hIL-2 from inclusion bodies represents a promising and attractive strategy for its commercial production. In this study, a 12-bioreactor 250 mL DASbox mini bioreactor system was employed to develop a model using Response Surface Methodology (RSM) aimed at understanding the impacts of cultivation parameters on cell growth and product yiled as well as maximizing hIL-2 production. The optimized process was further validated in a 5-L stirred tank bioreactor: an induction temperature of 31.3°C, an isopropyl β-D-1-thiogalactopyranoside (IPTG) concentration of 1.0 mM, a post-induction feeding rate of 103.80 mL/h, and an induction OD<sub>600</sub> of 150.0. The maximum hIL-2 titer achieved 4.90 g/L, which correlated well with the predicted range. Subsequent solubilization and in vitro refolding process resulted in hIL-2 purity exceeding 99 %, and a the bioactivity of 0.88 × 10<sup>7</sup> U/mL, comparable to that of commercial products. This study demonstrates the effectiveness of optimizing cultivation parameters to enhance hIL-2 yield and highlights the value of employing statistical methods as a powerful tool to guide manufacturing operations in recombinant protein production.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"150 ","pages":"Pages 265-275"},"PeriodicalIF":3.7,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143094479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0