Process Biochemistry最新文献_第5页

Beyond conductivity: Specific surface area and surface functional groups as determinants for chain elongation on carbon materials 超越电导率：比表面积和表面官能团作为碳材料链延伸的决定因素

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-02-01 Epub Date: 2025-12-10 DOI: 10.1016/j.procbio.2025.12.007

Yuhao Liu , Lei Zhao , Haiyang Lv , Xinyi Wang , Long Chen , Haihua Li , Ruihua Li , Xin Du , Shuli Liu , Haiping Gu

{"title":"Beyond conductivity: Specific surface area and surface functional groups as determinants for chain elongation on carbon materials","authors":"Yuhao Liu , Lei Zhao , Haiyang Lv , Xinyi Wang , Long Chen , Haihua Li , Ruihua Li , Xin Du , Shuli Liu , Haiping Gu","doi":"10.1016/j.procbio.2025.12.007","DOIUrl":"10.1016/j.procbio.2025.12.007","url":null,"abstract":"<div><div>In this study, bamboo charcoal, coconut shell charcoal, flake graphite powder, and graphene were used as mediators to investigate the roles of electrical conductivity, specific surface area (SSA), and surface functional groups in the chain elongation (CE) for medium-chain fatty acid (MCFAs) synthesis. The study compared biomass charcoals (bamboo charcoal and coconut shell charcoal) and graphitic materials (flake graphite powder and graphene), controlling their total SSA at 1800 m<sup>2</sup>/L and 100 m<sup>2</sup>/L, respectively. Both bamboo and coconut shell charcoal significantly promoted MCFAs synthesis, with the latter showing superior performance, likely due to its higher conductivity and richer functional groups. In contrast, graphitic materials, despite their much higher conductivity, showed limited to no promotion of MCFAs production; flake graphite powder mainly enhanced butyrate synthesis. These results demonstrate that for different types of carbon materials, high conductivity does not necessarily lead to an increase in CE. The key functional microorganism, <em>Clostridium_sensu_stricto_12</em>, showed significant correlation with the yield, selectivity, and electron transfer efficiency of MCFAs, and its abundance also showed a notable correlation with the SSA and surface functional group content of the carbon materials, but not their conductivity. Metagenomic analysis revealed that in the CE system, both the reverse β-oxidation (RBO) pathway and the fatty acid biosynthesis (FAB) pathway are present, with the RBO pathway being the dominant pathway.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"161 ","pages":"Pages 164-176"},"PeriodicalIF":4.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145786507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Pectin-ZnO bionanocomposite membrane based on Annona muricata leaf extract for in vitro treatment of skin and soft tissue infections 基于番荔枝叶提取物的果胶- zno生物纳米复合膜体外治疗皮肤和软组织感染

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-02-01 Epub Date: 2025-11-28 DOI: 10.1016/j.procbio.2025.11.013

Suganya Ilango , Biswaranjan Paital , Nirmaladevi Ramalingam , Tejasweta Bhuyan , Tapas Ranjan Behera , Dipak Kumar Sahoo

{"title":"Pectin-ZnO bionanocomposite membrane based on Annona muricata leaf extract for in vitro treatment of skin and soft tissue infections","authors":"Suganya Ilango , Biswaranjan Paital , Nirmaladevi Ramalingam , Tejasweta Bhuyan , Tapas Ranjan Behera , Dipak Kumar Sahoo","doi":"10.1016/j.procbio.2025.11.013","DOIUrl":"10.1016/j.procbio.2025.11.013","url":null,"abstract":"<div><div>Bionanocomposite membranes with increased durability are emerged as promising therapeutic candidates for the treatment of skin and soft tissue infections (SSTIs). In this study, a pectin-ZnO bionanocomposite membrane was synthesized using <em>Annona muricata</em> leaf extract. The formulation was examined with 2.3 % pectin, 5 % glycerol, and green-synthesized ZnO nanoparticles at concentrations of 50, 100, 150, and 200 mg mL<sup>−1</sup>. The structural and functional characterization of the nanoparticles were confirmed using UV–visible spectroscopy (peak at 378 nm), FTIR, XRD (particle size: 18.41 nm), and SEM (particle size: 16.4 nm). The membrane exhibited strong antibacterial activity, being the highest inhibition observed against <em>Streptococcus</em> spp. (with the observed zone of inhibition of 24–26 mm at 200 mg mL-<sup>1</sup> ZnO and minimum inhibitory concentration of 65.68 µg/mL<sup>−1</sup>), followed by <em>Staphylococcus aureus</em> and <em>S. epidermidis</em>. Solubility tests revealed excellent hygroscopic properties, enhancing its potential as a bioadhesive plaster. These results indicate that the eco-friendly biodegradable membrane can be used as a promising topical formulation for the treatment for SSTIs.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"161 ","pages":"Pages 85-98"},"PeriodicalIF":4.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145681643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Composition analysis and prediction of sulfuric acid-assisted steam-exploded corn stover slurry: Guiding parameters selection 硫酸辅助蒸汽爆破玉米秸秆浆料成分分析与预测：指导参数选择

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-02-01 Epub Date: 2025-12-06 DOI: 10.1016/j.procbio.2025.12.004

Yinghui He, Hui Zhang, Kaili Ding, Xueyan Liang, Xiangjie Zuo, Nan Zhang, Li Liu, Suan Shi, Lujia Han, Weihua Xiao

{"title":"Composition analysis and prediction of sulfuric acid-assisted steam-exploded corn stover slurry: Guiding parameters selection","authors":"Yinghui He, Hui Zhang, Kaili Ding, Xueyan Liang, Xiangjie Zuo, Nan Zhang, Li Liu, Suan Shi, Lujia Han, Weihua Xiao","doi":"10.1016/j.procbio.2025.12.004","DOIUrl":"10.1016/j.procbio.2025.12.004","url":null,"abstract":"<div><div>The slurry compositions of sulfuric acid-assisted steam explosion (SASE) corn stover are strongly dependent on process parameters. Preliminary assessment of these compositions facilitates the rapid selection of SASE parameters. In this study, curve fitting and support vector regression (SVR) were employed to elucidate the variation trends of components in SASE slurry and achieve prediction. Curve fittings clarified the variation patterns of components with the combined severity factor (CSF), exhibiting high goodness-of-fit (<em>R</em><sup>2</sup> > 0.942 and RMSE < 0.287), and were utilized to identify optimal CSF balancing inhibitors, cellulose, and xylooligosaccharides (XOS). The co-production of 8.20 g/L XOS and 40.48 g/L enzymatic hydrolysis sugars was achieved. Additionally, the SVR model realized the multivariate nonlinear fitting of pressure, time, and acid concentration, and analyzed the contribution degree of operating parameters, thereby providing more robust guidance for parameter selection. The SVR model demonstrated exceptional predictive performance, achieving <em>R</em><sup><em>2</em></sup> > 0.969 and RMSE < 0<sup>.</sup>436. The variation between experimental values and predicted values was less than 20 %. In conclusion, the integrated framework of curve fitting and SVR offers a reliable, rapid approach that not only simplifies the parameter optimization process of SASE pretreatment but also provides a practical basis for industrial application.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"161 ","pages":"Pages 126-138"},"PeriodicalIF":4.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145736804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Removal selectivity of His6-SUMO-Tag in recombinant protein production based on skin cell behaviors 基于皮肤细胞行为的His6-SUMO-Tag在重组蛋白生产中的去除选择性

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-02-01 Epub Date: 2025-11-13 DOI: 10.1016/j.procbio.2025.11.006

Zilong Zhao , Weigang Yuwen , Linlin Qu , Daidi Fan

{"title":"Removal selectivity of His6-SUMO-Tag in recombinant protein production based on skin cell behaviors","authors":"Zilong Zhao , Weigang Yuwen , Linlin Qu , Daidi Fan","doi":"10.1016/j.procbio.2025.11.006","DOIUrl":"10.1016/j.procbio.2025.11.006","url":null,"abstract":"<div><div>His<sub>6</sub>-SUMO-tag, widely used in recombinant protein production for enhancing solubility and folding, has seen increased use due to its removable nature via SUMO proteases. However, the necessity of removing the His<sub>6</sub>-SUMO-tag during the production of recombinant proteins remains controversial in various studies, primarily due to differing views on whether the His<sub>6</sub>-SUMO-tag may impede the functional activity of the recombinant protein. This study aims to validate His6-SUMO-tag's effects on cell proliferation, adhesion, and migration, and explore its role in skin cell metabolism using transcriptomics. His<sub>6</sub>-SUMO-tag produced in <em>Escherichia coli</em> was purified with Ni-IDA Magbeads, characterized, and yielded 0.25 ± 0.08 g/L. Optimal proliferative concentrations for HaCaT and HSF cells were 1 mg/mL and 0.5 mg/mL, respectively, upregulating MKI67 and PCNA gene expression. His<sub>6</sub>-SUMO-tag may have complex adhesion mechanisms with varying effects on cell types and no significant migratory effect on skin cells. Its impact on gene transcription in HaCaT and HSF cells was similar, focusing on immune and inflammatory responses. This indicates that the influence of the His<sub>6</sub>-SUMO-tag on the activity of recombinant proteins that do not relate to immune-activation is minimal, suggesting that it may optionally be retained without removal. This study underscores His<sub>6</sub>-SUMO-tag's potential in assessing cellular activity and supports its use in protein and fermentation engineering.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"161 ","pages":"Pages 1-9"},"PeriodicalIF":4.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145519249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chitosan-modified carboxylated magnetic nanoparticles immobilized glycosidases for the production of icaritin 壳聚糖修饰羧化磁性纳米颗粒固定化糖苷酶制备淫羊藿苷

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-02-01 Epub Date: 2025-11-15 DOI: 10.1016/j.procbio.2025.11.007

Yuxuan Zhao , Ye Li , Wenting Fei , Hao Liang

{"title":"Chitosan-modified carboxylated magnetic nanoparticles immobilized glycosidases for the production of icaritin","authors":"Yuxuan Zhao , Ye Li , Wenting Fei , Hao Liang","doi":"10.1016/j.procbio.2025.11.007","DOIUrl":"10.1016/j.procbio.2025.11.007","url":null,"abstract":"<div><div>In recent years, nanomaterials have emerged as prominent carriers in biomedical applications. This trend aims to address limitations inherent in traditional enzyme immobilization methods and reduce the costs associated with enzyme biocatalysts in industrial processes. In this study, we selected non-toxic magnetic nanoparticles (MNPs), distinguished by their exceptional biocompatibility, as immobilization carriers to overcome the inherent instability and non-reusability of free enzymes. The MNPs were coated with chitosan to form a protective layer, thereby significantly enhancing enzyme stability. This approach also circumvented the need for precipitants, thereby avoiding the associated damage observed in conventional cross-linking. Building upon this foundation, glucosidase and rhamnosidase were immobilized onto these modified MNPs for converting Epimedin C into Icaritin. Compared to conventional cross-linked enzyme aggregation techniques, this system exhibited superior catalytic efficiency. Furthermore, the immobilized enzyme system enables rapid magnetic separation of the reaction product. Notably, the immobilized enzymes retained approximately 70 % of their initial activity after eight reuse cycles, demonstrating significant potential for industrial-scale applications. These findings demonstrate the potential of chitosan-modified magnetic nanoparticles as efficient, reusable enzyme carriers for sustainable biocatalytic processes in pharmaceutical and industrial applications.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"161 ","pages":"Pages 75-84"},"PeriodicalIF":4.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145570702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immobilization of glutamate decarboxylase on tussah silk modified magnetic nanoparticles via laccase catalyzed oxidative coupling 漆酶催化氧化偶联固定化柞蚕丝磁性纳米颗粒上谷氨酸脱羧酶的研究

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-02-01 Epub Date: 2025-11-11 DOI: 10.1016/j.procbio.2025.11.004

Hanhan Xie , Kaixuan Liu , Yuanrui Gao, Ruofu Shi, Chengli Yang, Dali Li

{"title":"Immobilization of glutamate decarboxylase on tussah silk modified magnetic nanoparticles via laccase catalyzed oxidative coupling","authors":"Hanhan Xie , Kaixuan Liu , Yuanrui Gao, Ruofu Shi, Chengli Yang, Dali Li","doi":"10.1016/j.procbio.2025.11.004","DOIUrl":"10.1016/j.procbio.2025.11.004","url":null,"abstract":"<div><div>Gamma-aminobutyric acid (GABA), the primary inhibitory neurotransmitter in the central nervous system, exhibits multiple physiological functions. The GABA is synthesized through the decarboxylation of glutamic acid, a reaction catalyzed by the enzyme glutamate decarboxylase (GAD). In this study, magnetic nanoparticles were coated with tussah silk (TS) protein. The GAD was then immobilized via laccase-catalyzed oxidative coupling. Characterization revealed that the TS protein coated magnetic nanoparticles exhibited spherical morphology with rough surfaces, an average particle size of 27 nm, and excellent magnetic separation capability. Compared to the free GAD, the immobilized GAD exhibited significantly enhanced pH and thermal stability. After 15 days at 4 °C, the immobilized GAD retained 76.3 % relative activity, whereas the free enzyme showed a complete loss of activity. Furthermore, the immobilized GAD maintained approximately 50 % residual activity after seven operational recycles. This work successfully established an enzymatic crosslinking strategy for GAD immobilization on magnetic nanoparticles, providing both an efficient approach for GABA production and a novel methodology for enzyme immobilization technology.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"161 ","pages":"Pages 10-17"},"PeriodicalIF":4.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145519254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modelling growth-arrested production of polyhydroxybutyrate by Cupriavidus necator DSM 545 模拟Cupriavidus necator DSM 545生长停滞生产聚羟基丁酸酯

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-02-01 Epub Date: 2026-01-03 DOI: 10.1016/j.procbio.2025.12.018

Ernesto González, Alberto García-Martín, Juan Manuel Bolívar, Miguel Ladero, Victoria E. Santos

{"title":"Modelling growth-arrested production of polyhydroxybutyrate by Cupriavidus necator DSM 545","authors":"Ernesto González, Alberto García-Martín, Juan Manuel Bolívar, Miguel Ladero, Victoria E. Santos","doi":"10.1016/j.procbio.2025.12.018","DOIUrl":"10.1016/j.procbio.2025.12.018","url":null,"abstract":"<div><div>Production of polyhydroxyalkanoates has gained attention because they are biobased and biodegradable plastics. Among them, polyhydroxybutyrate (PHB) is the most common type of polyhydroxyalkanoate produced by several bacteria including <em>Cupriavidus necator</em>. Hence, this work aims to use resting cells of <em>C. necator</em> DSM 545 because this strategy is simpler than culture processes and this strain can assimilate glucose, a widely available monomer. <em>C. necator</em> DSM 545 was grown using a defined culture medium. The biomass was harvested and utilized to carry out resting cells experiments using phosphate buffer amended with glucose in different concentrations (2–20 g/L). Additional experiments include tests with different cell concentrations (1–8 g/L), shaking rates (150 and 180 rpm), and measurement of dissolved oxygen in 250 mL Erlenmeyer flasks to determinate the volumetric mass transfer coefficient. Experiments with resting cells showed PHB production while the PHB-free biomass remains constant. The glucose uptake rate has a lineal behavior regarding initial PHB-free biomass (q<sub>S</sub>=0.37 ± 0.01 g<sub>glucose</sub>/g<sub>PHB-free biomass</sub>·h) and a saturation trend regarding glucose concentration (K<sub>S</sub>=0.88 ± 0.05 g/L). The observed yield for PHB was 0.482 ± 0.003 g<sub>PHB</sub>/g<sub>glucose</sub> (Y<sub>P/S</sub>), coinciding with the maximum reported for the metabolic route. Measurements of dissolved oxygen showed that the process may be limited by the oxygen transfer rate.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"161 ","pages":"Pages 290-297"},"PeriodicalIF":4.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145924344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shortening composting period of swine waste: A two-step method combining rotary drum and vermicomposting with bulking agents 缩短猪粪堆肥周期：转鼓和蚯蚓堆肥加膨化剂两步法

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-02-01 Epub Date: 2025-12-18 DOI: 10.1016/j.procbio.2025.12.012

C. Lalthlansanga , Bijayananda Mohanty , Ajay S. Kalamdhad

{"title":"Shortening composting period of swine waste: A two-step method combining rotary drum and vermicomposting with bulking agents","authors":"C. Lalthlansanga , Bijayananda Mohanty , Ajay S. Kalamdhad","doi":"10.1016/j.procbio.2025.12.012","DOIUrl":"10.1016/j.procbio.2025.12.012","url":null,"abstract":"<div><div>A novel strategy is investigated to optimize swine dung composting time and quality by sequential integration of Rotary Drum Composting (RDC) and Vermicomposting technologies. The pre-thermophilic treatment for 7 days in the rotary drum composter (RDC), followed by 30 days of vermicomposting with bulking agents, significantly reduced the overall composting duration and improved the quality of the final bioproducts. Post vermicomposting, enhanced levels of TKN, TP, and TK were observed in the final compost. Thus, at the end of the 30th day, the treatment using rice straw RV3 (rice straw + swine waste) outperformed with TKN (2.54 %), TP (11.58 g kg<sup>−1</sup>) and TK (21.6 g kg<sup>−1</sup>). Furthermore, the final compost satisfied heavy metal concentration safety standards, minimizing possible environmental risks with final concentrations of Cu < 200 mg kg<sup>−1</sup>, Pb < 100 mg kg<sup>−1</sup> and Zn < 300 mg kg<sup>−1</sup> for the three reactors. This integrated composting technology offers a fast and efficient solution for swine waste composting, enhancing earthworm population growth, making it a viable and sustainable agriculture option.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"161 ","pages":"Pages 236-248"},"PeriodicalIF":4.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145839656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced ergosterol biosynthesis in Saccharomyces cerevisiae via rational multigenetic modification and two-stage fermentation 通过合理的多基因修饰和两段发酵增强酿酒酵母麦角甾醇的生物合成

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-02-01 Epub Date: 2025-11-19 DOI: 10.1016/j.procbio.2025.11.011

Yufeng Xie , Chenxu Fan , Minhao Piao , Longgang Jia

{"title":"Enhanced ergosterol biosynthesis in Saccharomyces cerevisiae via rational multigenetic modification and two-stage fermentation","authors":"Yufeng Xie , Chenxu Fan , Minhao Piao , Longgang Jia","doi":"10.1016/j.procbio.2025.11.011","DOIUrl":"10.1016/j.procbio.2025.11.011","url":null,"abstract":"<div><div>Ergosterol is a valuable precursor for synthesis of vitamin D2. However, low-cost production of ergosterol by yeast cell fermentation is still a major challenge due to low ergosterol content in production strains. Here, we screened a high-yield ergosterol producing <em>Saccharomyces cerevisiae</em> strain YX5 that shown to accumulate the highest contents of ergosterol [9.6 mg/g dry cell weight (DCW)]. Overexpression in strain YX5 of the truncated <em>tHMG1</em> encoding the rate-limiting enzyme HMG-CoA reductase 1 in the ergosterol biosynthesis pathway resulted in a 64 % increase in ergosterol yield (15.7 mg/g DCW). Combined overexpression of <em>tHMG1</em> and <em>ERG1</em> encoding the squalene epoxidase or <em>ERG11</em> encoding the sterol C14-demethylase further enhanced the ergosterol yields by 71 % (26.8 mg/g DCW), 34 % (21.0 mg/g DCW), respectively. Simultaneous overexpression of <em>tHMG1</em>, <em>ERG1</em> and <em>ERG11</em> in strain YX5 led to ergosterol yield of 36.2 mg/g DCW, a 276.8 % increase compared to the parental strain. High-cell-density fermentation for strain YX5-tHMG1<em>/</em>ERG1/ERG11 based on a two-stage feeding strategy led to ergosterol titers of 2077.0 mg/L and ergosterol contents of 35.9 mg/g DCW. The engineered strain YX5-tHMG1<em>/</em>ERG1/ERG11 from this study could be a useful chassis for further engineering of more efficient ergosterol production strains.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"161 ","pages":"Pages 18-25"},"PeriodicalIF":4.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145570632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural characterization and anti-atherosclerotic potential of low molecular weight fucoidan isolated from Ishige okamurae 石重冈村菌低分子量岩藻糖聚糖的结构表征及抗动脉粥样硬化潜力

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-02-01 Epub Date: 2025-12-26 DOI: 10.1016/j.procbio.2025.12.016

Chathuri Kaushalya Marasinghe , Jun-Geon Je , Bomi Ryu , Jae-Young Je

{"title":"Structural characterization and anti-atherosclerotic potential of low molecular weight fucoidan isolated from Ishige okamurae","authors":"Chathuri Kaushalya Marasinghe , Jun-Geon Je , Bomi Ryu , Jae-Young Je","doi":"10.1016/j.procbio.2025.12.016","DOIUrl":"10.1016/j.procbio.2025.12.016","url":null,"abstract":"<div><div>In this study, the anti-atherosclerotic potential of <em>Ishige okamurae</em>-derived fucoidan (IOF) fractions was evaluated, with F4 fraction identified as the most bioactive due to its high fucose content and superior bioactivity in <em>in vitro</em> and <em>in vivo</em> assays. The F4 fraction inhibited foam cell formation in oxLDL-treated RAW264.7 macrophages by reducing intracellular lipid accumulation, total cholesterol content, cholesterol influx, and associated protein expression. Conversely, it enhanced cholesterol efflux by upregulating efflux-related proteins via the PPAR-γ/LXR-α signaling pathway, mediated through AMPK phosphorylation. Validation using human aortic smooth muscle cells (HASMCs) confirmed the foam cell formation inhibitory effects of the F4 fraction in human-derived models. The F4 fraction also demonstrated significant anti-inflammatory properties by suppressing inflammatory mediators, including nitric oxide (NO) and downregulating iNOS and COX-2 protein expression. Additionally, it inhibited NF-κB nuclear translocation, thereby attenuating inflammatory signaling. Furthermore, the F4 fraction exhibited potent antioxidant activity by activating the HO-1/Nrf2 signaling pathway, enhancing cellular defenses against oxidative stress. These findings highlight the multifaceted therapeutic potential of the F4 fraction in mitigating key pathological processes of atherosclerosis, including foam cell formation, inflammation, and oxidative stress, suggesting its promise as a novel anti-atherosclerotic agent.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"161 ","pages":"Pages 269-282"},"PeriodicalIF":4.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145883313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0