Small yellow ginger extract: Preparation, characterization, antioxidant properties, and protective activities against alcohol-induced HepG2 cell injury

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry Pub Date : 2025-07-17 DOI:10.1016/j.procbio.2025.07.011

Qiubian Yang , Peihang Chen , Ruimin Zhong , Jianyin Miao

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引用次数: 0

Abstract

Small yellow ginger (Zingiber officinale Roscoe) is a traditional medicinal and edible plant. In this study, response surface methodology was used to optimize the preparation process of small yellow ginger extract using total polyphenols content as an indicator, and the chemical composition, antioxidant capacity and protective effects of the extract on alcohol-induced injury in HepG2 cells were investigated. The results implied that the best conditions to reach the highest value of total polyphenols content (10.59 mg GAE/g) were an extraction time of 32 min, ethanol concentration of 71 % v/v, solid-liquid ratio of 1:19 g/mL, and temperature of 60 ℃. The 67 main components were identified by UPLC-MS/MS. The extract exhibited excellent DPPH and ABTS radical scavenging effects (IC₅₀ of 0.11 mg/mL and 28.83 μg/mL, respectively) and ORAC values (758.36 μmol TE/g lyophilized powder). The CAA assay indicated that the extract possessed potent antioxidant activity (46.22 μg/mL) against HepG2 cells. Furthermore, the extract exhibited a significant protective effect on ethanol-induced HepG2 cell injury. This study provides a scientific basis for the development of functional foods and natural antioxidants based on small yellow ginger and promotes its application in the prevention and treatment of alcoholic liver disease.

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小黄姜提取物的制备、表征、抗氧化性能和对酒精诱导的HepG2细胞损伤的保护作用

小黄姜（Zingiber officinale Roscoe）是一种传统药用和食用植物。本研究以总多酚含量为指标，采用响应面法优化小黄姜提取物的制备工艺，考察其化学成分、抗氧化能力及对HepG2细胞酒精性损伤的保护作用。结果表明，提取时间为32 min，乙醇浓度为71 % v/v，料液比为1:19 g/mL，温度为60℃，总多酚含量最高（10.59 mg GAE/g）的最佳条件为提取时间为32 min。通过UPLC-MS/MS对67种主要成分进行鉴定。提取物具有良好的DPPH和ABTS自由基清除率（IC50分别为0.11 mg/mL和28.83 μg/mL）和ORAC值（758.36 μmol TE/g冻干粉）。CAA测定表明，提取物对HepG2细胞具有较强的抗氧化活性（46.22 μg/mL）。此外，提取物对乙醇诱导的HepG2细胞损伤具有显著的保护作用。本研究为开发以小黄姜为基础的功能性食品和天然抗氧化剂提供了科学依据，促进了其在酒精性肝病防治中的应用。

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来源期刊

Process Biochemistry 生物-工程：化工

CiteScore

8.30

自引率

4.50%

发文量

374

审稿时长

53 days

期刊介绍： Process Biochemistry is an application-orientated research journal devoted to reporting advances with originality and novelty, in the science and technology of the processes involving bioactive molecules and living organisms. These processes concern the production of useful metabolites or materials, or the removal of toxic compounds using tools and methods of current biology and engineering. Its main areas of interest include novel bioprocesses and enabling technologies (such as nanobiotechnology, tissue engineering, directed evolution, metabolic engineering, systems biology, and synthetic biology) applicable in food (nutraceutical), healthcare (medical, pharmaceutical, cosmetic), energy (biofuels), environmental, and biorefinery industries and their underlying biological and engineering principles.