Process Biochemistry最新文献

Statistical optimization and kinetic modeling simulation for fibrinolytic enzyme production by Bacillus tropicus from mahewu, a traditional South African fermented food

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-03-20 DOI: 10.1016/j.procbio.2025.03.010

Chinmay Hazare , Prashant Bhagwat , Neeraj Kumar Singh , Suren Singh , Santhosh Pillai

{"title":"Statistical optimization and kinetic modeling simulation for fibrinolytic enzyme production by Bacillus tropicus from mahewu, a traditional South African fermented food","authors":"Chinmay Hazare , Prashant Bhagwat , Neeraj Kumar Singh , Suren Singh , Santhosh Pillai","doi":"10.1016/j.procbio.2025.03.010","DOIUrl":"10.1016/j.procbio.2025.03.010","url":null,"abstract":"<div><div>Cardiovascular diseases are a leading cause of global mortality, with existing thrombolytic drugs having side effects, short half-life, and high costs, warranting alternatives. Since the discovery of nattokinase from Japanese natto, fibrinolytic enzymes from fermented foods have been explored as promising thrombolytic agents. However, most research has focused on Asian fermented foods, with limited studies on African sources. In this study, <em>Bacillus tropicus</em> DUT M1, an extracellular fibrinolytic enzyme producer, was isolated for the first time from mahewu, an indigenous fermented food. Optimization was performed using a combination of OFAT and RSM. Glucose and casein were the optimal carbon and nitrogen sources identified through OFAT, while casein, meat extract, and pH were significant in the Plackett-Burman design. CCD optimized the medium to 28.23 g/L casein, 6.41 g/L meat extract, and pH 7.90, increasing enzyme production 13.9-fold, 12 U/mL to 167 U/mL. Kinetic modeling validated S-Gompertz as the best fit (R² = 0.97) compared to Logistic-1, Richards, and S- Weibull- 2 models. Fibrinolytic activity was confirmed via quantitative tube assay, fibrin zymography, and blood clot lysis experiments. These findings establish mahewu as a novel source of fibrinolytic enzyme producers, highlighting the therapeutic and industrial potential of African fermented foods.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"153 ","pages":"Pages 122-134"},"PeriodicalIF":3.7,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143681332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The utilization of biopolymer hydrogels to encapsulate and protect probiotics in foods

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-03-17 DOI: 10.1016/j.procbio.2025.03.008

Great Iruoghene Edo , Alice Njolke Mafe , Patrick Othuke Akpoghelie , Tayser Sumer Gaaz , Emad Yousif , Obadiah Saveni Yusuf , Endurance Fegor Isoje , Ufuoma Augustina Igbuku , Rapheal Ajiri Opiti , Jimoh Lawal Ayinla , Arthur Efeoghene Athan Essaghah , Dina S. Ahmed , Huzaifa Umar

{"title":"The utilization of biopolymer hydrogels to encapsulate and protect probiotics in foods","authors":"Great Iruoghene Edo , Alice Njolke Mafe , Patrick Othuke Akpoghelie , Tayser Sumer Gaaz , Emad Yousif , Obadiah Saveni Yusuf , Endurance Fegor Isoje , Ufuoma Augustina Igbuku , Rapheal Ajiri Opiti , Jimoh Lawal Ayinla , Arthur Efeoghene Athan Essaghah , Dina S. Ahmed , Huzaifa Umar","doi":"10.1016/j.procbio.2025.03.008","DOIUrl":"10.1016/j.procbio.2025.03.008","url":null,"abstract":"<div><div>Biopolymer hydrogels have emerged as a transformative technology for enhancing the stability, delivery, and efficacy of probiotics in food systems. These hydrogels provide robust protection against environmental stressors such as pH fluctuations, temperature extremes, and moisture, ensuring probiotic survival during processing, storage, and digestion. Their versatility allows for seamless integration into a wide range of food matrices, including beverages, baked goods, and plant-based products, resolving the growing consumer demand for functional foods that promote gut health. This review explores the potential of biopolymer hydrogels in probiotic encapsulation, emphasizing their role in controlled release and targeted delivery within the gastrointestinal tract. Despite their advantages, hurdles remain in optimizing formulations, refining encapsulation techniques, and expanding their application to non-traditional food systems. Future research should focus on strain-specific hydrogel designs, innovative probiotic carriers, and advanced delivery mechanisms to maximize probiotic functionality. From an industrial and commercial perspective, continued advancements in hydrogel technology could revolutionize the functional food and nutraceutical industries. By improving probiotic stability and bioavailability, biopolymer hydrogels present a promising avenue for developing next-generation health-promoting foods. This review highlights the latest advancements in hydrogel technology and its applications, underscoring its potential to shape the future of functional foods and contribute to a more sustainable and health-conscious food industry.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"153 ","pages":"Pages 66-91"},"PeriodicalIF":3.7,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143643165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipid-lowering effects and metabolomic investigation of Polygonatum sibiricum fermented by Lactobacillus plantarum NX-1

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-03-17 DOI: 10.1016/j.procbio.2025.03.007

Wenjuan Cao , Yan Liu , Zhao Zhang , Xiaodong Xu

引用次数: 0

Bioethanol production from delignified rice straw by using a novel crude recombinant enzyme cocktail in pre-saccharification simultaneous saccharification and fermentation process

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-03-11 DOI: 10.1016/j.procbio.2025.03.005

Premeshworii Devi Maibam , Arun Goyal

{"title":"Bioethanol production from delignified rice straw by using a novel crude recombinant enzyme cocktail in pre-saccharification simultaneous saccharification and fermentation process","authors":"Premeshworii Devi Maibam , Arun Goyal","doi":"10.1016/j.procbio.2025.03.005","DOIUrl":"10.1016/j.procbio.2025.03.005","url":null,"abstract":"<div><div>This study highlights the problem of differences in the optimum temperatures for the operation of enzymatic saccharification and fermentation processes for lignocellulosic bioethanol production. Therefore, to address this problem, the pre-saccharification simultaneous saccharification and fermentation (PSSF) was carried out for bioethanol production from deep eutectic pretreated rice straw using crude recombinant bacterial enzyme cocktail and the process was statistically optimized by Central Composite Design approach. The three variables <em>viz.</em> pre-saccharification time, enzyme dosage and fermentation temperature were considered for maximizing the ethanol yield and productivity. Statistical analysis of PSSF optimization inferred that low pre-saccharification time (9.6–15 h) and crude enzyme dosage (above 60 FPU/g) significantly influence higher ethanol yield and productivity. Statistical optimization resulted in optimum conditions: 12.2 h pre-saccharification time, crude enzyme dosage (83.7 FPU/g), fermentation temperature (33.7°C) with predicted ethanol yield, 0.35 g/g and productivity, 0.63 g/L/h. The validation of the PSSF process at bioreactor-level in 1.0 L working volume under optimized conditions gave ethanol yield, 0.37 g/g and productivity, 0.82 g/L/h resulting in an overall yield of 182 L ethanol/tonne raw-RS. This study shows the applicability of crude recombinant enzyme cocktail preparation and the modeled PSSF process as a promising industrial approach for bioethanol production using rice straw.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"153 ","pages":"Pages 26-33"},"PeriodicalIF":3.7,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143628261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cracking the lignocellulosic code: Comparative insights into various pretreatment techniques for enhanced degradation in cattle manure

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-03-11 DOI: 10.1016/j.procbio.2025.03.006

Qian Zhang , Zhixuan Yin , Deming He , Wenjun Yan , Changqing Liu

{"title":"Cracking the lignocellulosic code: Comparative insights into various pretreatment techniques for enhanced degradation in cattle manure","authors":"Qian Zhang , Zhixuan Yin , Deming He , Wenjun Yan , Changqing Liu","doi":"10.1016/j.procbio.2025.03.006","DOIUrl":"10.1016/j.procbio.2025.03.006","url":null,"abstract":"<div><div>High content of refractory lignocelluloses in cattle manure has long been a major bottleneck, severely hindering its anaerobic biogas production potential. In this study, a comparative analysis was conducted to evaluate the effects of thermal pretreatment, alkali pretreatment, and their combined approach on the degradation of lignocellulosic components within cattle manure. The results revealed that thermal-alkali pretreatment achieved the most significant improvement in lignocelluloses degradation, outperforming both alkali-only and thermal-only pretreatments, particularly in terms of lignin degradation. Notably, NaOH dosage was found to have a more pronounced impact on degradation efficiency than temperature. Under the most favorable pretreatment conditions, which involved a 2 % NaOH dosage and a temperature of 110 °C, the degradation efficiencies of hemicellulose, cellulose, and lignin reached 51.3 %, 26.5 %, and 45.7 %, respectively. This marked improvement led to the release of soluble biodegradable organics, including protein and carbohydrate, thereby enhancing the bioconversion potential in subsequent processes. Moreover, the release of ammonia and phosphate further elevated the possibility of efficient nutrients recovery, adding additional value to the pretreatment. Scanning electron microscopy analysis further revealed substantial alterations to the morphological characteristics of coarse fibers in cattle manure after the pretreatments, providing visual evidence of the enhanced degradation of lignocellulosic structure.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"153 ","pages":"Pages 34-46"},"PeriodicalIF":3.7,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143628157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbiological mechanisms and effects of dissimilatory iron reduction on combined contaminants bio-transformation: A review

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-03-11 DOI: 10.1016/j.procbio.2025.03.004

Yue Yang, Haibo Li, Yajue Yuan, Yaqin Zheng, Tianle Zhang, Yichen Wu

{"title":"Microbiological mechanisms and effects of dissimilatory iron reduction on combined contaminants bio-transformation: A review","authors":"Yue Yang, Haibo Li, Yajue Yuan, Yaqin Zheng, Tianle Zhang, Yichen Wu","doi":"10.1016/j.procbio.2025.03.004","DOIUrl":"10.1016/j.procbio.2025.03.004","url":null,"abstract":"<div><div>Although dissimilatory iron-reducing bacteria (DIRB) has been executed to degrade organic matters and reduce heavy metals simultaneously due to its unique extracellular electron transfer (EET) mechanism, there is still a challenge to define a synergistic way for combined pollution control. Organic matters and heavy metals can serve as electron donators and acceptors respectively, and electrons will be indirectly transferred interactively <em>via</em> bio-transmembrane. Many literatures indicated that dissimilatory iron reduction (DIR) can induce the ferric redox formation, and consequently promote the bio-metabolic activity, which could stimulate a series of beneficial reactions, such as bio-Fenton. Unfortunately, up to now, little was known about how and why DIRB can execute the above bio-transformation. A deep insight into EET and a systematic illustration of the DIRB-driving reactions will be helpful for understanding how DIRB works. The objective of this review is to focus on clarifying the EET of typical DIRB and revealing how DIR strengthen the combined pollution treatment. Most importantly, several gaps in the application of DIRB have been identified, along with proposed solutions, offering an innovative approach to addressing these challenges.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"153 ","pages":"Pages 47-65"},"PeriodicalIF":3.7,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143636442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trehalose-powered membraneless enzymatic fuel cell based on flexible alginate composite hydrogel bioelectrodes

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-03-07 DOI: 10.1016/j.procbio.2025.03.003

Said Nawab , Chong Sha , Syed Bilal Shah , Hareef Ahmed Keerio , Zhiguang Zhu , Yang-Chun Yong

{"title":"Trehalose-powered membraneless enzymatic fuel cell based on flexible alginate composite hydrogel bioelectrodes","authors":"Said Nawab , Chong Sha , Syed Bilal Shah , Hareef Ahmed Keerio , Zhiguang Zhu , Yang-Chun Yong","doi":"10.1016/j.procbio.2025.03.003","DOIUrl":"10.1016/j.procbio.2025.03.003","url":null,"abstract":"<div><div>This study investigated the design and optimization of a membraneless flexible gel-type enzymatic biofuel cell (EBFC) driven by trehalose for electricity generation. The EBFC was fabricated by printing bioelectrodes on non-woven fabrics encapsulating the enzymes in alginate composite hydrogel, and a conductive carbon layer was incorporated to improve electrochemical transfer. Optimization of trehalase loading revealed that a maximum power density of 4.69 μW/cm<sup>2</sup> was obtained with a trehalase loading of 3.5 U per 6 mL of 50 mM trehalose. Further optimization of the GOx-to-hydrogel ratio in the anode resulted in a maximum power density of 7.45 μW/cm<sup>2</sup>. Evaluations at different trehalose concentrations demonstrated proportionate increases in voltage, power density, and current density, achieving the highest power density of 10.74 μW/cm<sup>2</sup> at 100 mM. Connecting three EBFCs in series considerably enhanced power output, achieving a four-fold improvement in power density to 41.32 μW/cm<sup>2</sup> compared to a single cell. The EBFC also generated power from cockroach hemolymph, with a maximum power density of 8.05 μW/cm<sup>2</sup>. These results highlight that this flexible gel-type EBFC has the potential to be employed in the development of both trehalose-powered and glucose-powered EBFC.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"153 ","pages":"Pages 18-25"},"PeriodicalIF":3.7,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143577981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving 3′-sialyllactose biosynthesis in Escherichia coli by engineering Neisseria meningitidis 406Y α2,3-sialyltransferase

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-03-07 DOI: 10.1016/j.procbio.2025.03.002

Qian Lin , Yuanlin Liu , Hao Wang , Zhaolin Huang , Wenbo Zhang , Mian Sheng , Yingying Zhu , Ruiyan Wang , Wanmeng Mu

{"title":"Improving 3′-sialyllactose biosynthesis in Escherichia coli by engineering Neisseria meningitidis 406Y α2,3-sialyltransferase","authors":"Qian Lin , Yuanlin Liu , Hao Wang , Zhaolin Huang , Wenbo Zhang , Mian Sheng , Yingying Zhu , Ruiyan Wang , Wanmeng Mu","doi":"10.1016/j.procbio.2025.03.002","DOIUrl":"10.1016/j.procbio.2025.03.002","url":null,"abstract":"<div><div>3′-Sialyllactose (3′-SL, Neu5Ac<em>α</em>2,3 Gal<em>β</em>1,4Glc), a prominent sialylated human milk oligosaccharide (HMO), has attracted significant attention due to its diverse physiological properties. The efficient <em>α</em>2,3-sialyltransferase (<em>α</em>2,3-SiaT) is crucial for the biosynthesis of 3′-SL. In this study, the 3′-SL biosynthetic pathway was constructed in EZAK (<em>E. coli</em> BL21(DE3) Δ<em>lacZ</em>Δ<em>nanA</em>Δ<em>nanK</em>). 406NST, which exhibits a high 3′-SL yield and low by-product formation, was chosen for molecular modification. Five amino acid differences between 406NST and NST were targeted for site-directed mutagenesis. Subsequently, saturation mutagenesis was carried out at the D40 position, followed by superimposed multipoint mutagenesis to generate the optimal strain Z28 (406NST-D40R-N113D-P120H), resulting in an extracellular 3′-SL yield of 4.67 g/L in shake flasks. In a 5 L bioreactor, the extracellular 3′-SL yield reached 29.54 g/L, achieving an overall 3′-SL yield of 0.52 g/L/h and a lactose conversion yield of 0.62 mol 3′-SL/mol. In conclusion, a highly active and specific <em>α</em>2,3-SiaT was successfully constructed, significantly improving the yield of 3′-SL.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"153 ","pages":"Pages 11-17"},"PeriodicalIF":3.7,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143577980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A higher C/N ratio enhances nitrogen treatment and the biodiesel characteristics of algal-bacterial bioflocs derived from aquaculture wastewater

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-03-05 DOI: 10.1016/j.procbio.2025.02.007

Fangzheng Hu , Ruoyu Chen , Yao Li , Dachuan Sun , Hongxin Tan

{"title":"A higher C/N ratio enhances nitrogen treatment and the biodiesel characteristics of algal-bacterial bioflocs derived from aquaculture wastewater","authors":"Fangzheng Hu , Ruoyu Chen , Yao Li , Dachuan Sun , Hongxin Tan","doi":"10.1016/j.procbio.2025.02.007","DOIUrl":"10.1016/j.procbio.2025.02.007","url":null,"abstract":"<div><div>One of the major issues facing intensive aquaculture is controlling pollution and converting nutrients in the effluent into usable biomass. This study investigated the generation of algal-bacterial bioflocs (ABBs) from <em>Chlorella pyrenoidosa</em> at different C/N ratios in aquaculture wastewater supplemented with glucose. Subsequently, we evaluated the nitrogen and phosphorus metabolism of this biomass, analyzed nutrient composition and potential as a biodiesel, conducted a thorough analysis of the microbial community. During the ABBs production, higher C/N ratio promoted the utilization of various forms of nitrogen, but resulted in more nitrogen losses. ABBs produced at high C/N ratio was more favorable for nitrogen conversion in light and dark conditions, especially at C/N 15, but unfavorable for crude protein (35.08 ± 1.89 %) and crude fat (13.01 ± 1.79 %) accumulation. Additionally, ABBs produced with glucose supplementation exhibited a higher cetane number, which is advantageous for producing eco-friendly, high-performance biodiesel. The high-throughput sequencing analysis demonstrated a significant abundance of <em>Pesudomonas</em> and <em>Bacillus</em> in high C/N ratio groups, which explained the higher removal efficiency and removal rate of nitrogen from wastewater. In conclusion, the addition of carbon sources improved both nitrogen removal and biodiesel performance of ABBs.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"153 ","pages":"Pages 1-10"},"PeriodicalIF":3.7,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143577982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impact mechanism of Manno-oligosaccharides on ammonia oxidation and microbial communities during aerobic composting

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-03-03 DOI: 10.1016/j.procbio.2025.03.001

Manli Duan , Mingxiu Li , Risheng Li , Xin Zhang , Xu Guo , Yuhua Zhang

{"title":"The impact mechanism of Manno-oligosaccharides on ammonia oxidation and microbial communities during aerobic composting","authors":"Manli Duan , Mingxiu Li , Risheng Li , Xin Zhang , Xu Guo , Yuhua Zhang","doi":"10.1016/j.procbio.2025.03.001","DOIUrl":"10.1016/j.procbio.2025.03.001","url":null,"abstract":"<div><div>The ammonium oxidation process is a critical step in the nitrogen cycle during aerobic composting, affecting the efficiency of nitrogen transformation and the quality of the compost. This study employed quantitative PCR and high-throughput sequencing to investigate the effects of different concentrations of Manno-oligosaccharides (MOS) (0.1 %, 0.5 %, 1.0 %, and 2.0 %) on nitrogen dynamics and ammonia-oxidizing microbial communities in composting cattle manure and straw, with a control. The results indicated that different concentrations of MOS increased the peak temperatures during the thermophilic stage of composting to above 60°C, higher than that of the CK (57.4°C), while ensuring that all treatments met the requirements for harmless disposal. Particularly, the germination index (GI) of the 0.5 % manno-oligosaccharide treatment reached 109.3 %, demonstrating excellent treatment efficacy. The 0.5 % manno-oligosaccharide treatment significantly increased the NO<sub>3</sub><sup>-</sup>-N content in compost (<em>P</em> < 0.05). The copy number of AOB <em>amoA</em> gene in the M0.5 and M1 treatments during the compost maturation stage was significantly higher than that in other treatments. <em>Norank_p_environmental_samples</em> and <em>Nitrosomonas</em> are the dominant microorganisms driving ammonia oxidation in the compost. Especially in the treatment with 0.5 % MOS, they play a crucial role in the immobilization of ammonia nitrogen. Structural equation modeling (SEM) analysis showed a significant positive correlation between AOB <em>amoA</em> genes and NO<sub>3</sub><sup>-</sup>-N in the 0.5 % oligosaccharide treatment, indicating effective promotion of nitrogen conversion by ammonia-oxidizing bacteria in the compost. These findings highlight the potential of manno-oligosaccharide as a biological modifier to improve composting efficiency and nitrogen management.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"153 ","pages":"Pages 92-101"},"PeriodicalIF":3.7,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143681335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0