Process Biochemistry最新文献

Bioconversion of phytosterols to 9-hydroxy-3-oxo-4-pregnene-20-carboxylic acid methyl ester by rerouting of phytosterol degradation pathways in Mycolicibacterium neoaurum 植物甾醇在新金黄色分枝杆菌中转化为9-羟基-3-氧-4-孕烯-20-羧酸甲酯

IF 4 3区生物学

Process Biochemistry Pub Date : 2025-09-26 DOI: 10.1016/j.procbio.2025.09.012

Xinxin Chen , Xiaohan Jiang , Bo Zhang , Zhiqiang Liu , Yuguo Zheng

{"title":"Bioconversion of phytosterols to 9-hydroxy-3-oxo-4-pregnene-20-carboxylic acid methyl ester by rerouting of phytosterol degradation pathways in Mycolicibacterium neoaurum","authors":"Xinxin Chen , Xiaohan Jiang , Bo Zhang , Zhiqiang Liu , Yuguo Zheng","doi":"10.1016/j.procbio.2025.09.012","DOIUrl":"10.1016/j.procbio.2025.09.012","url":null,"abstract":"<div><div>9-Hydroxy-3-oxo-4-pregnene-20-carboxylic acid methyl ester (9-OH-3-OPCM) is a representative C-22 steroid precursor in the synthesis of progestational based drug. However, 9-OH-3-OPCM production from phytosterol in <em>Mycolicibacterium neoaurum</em> (<em>M</em>. <em>neoaurum</em>) remains underdeveloped due to the complexity of the steroid metabolic pathway. In this study, we genetically modified the phytosterol metabolism in <em>M</em>. <em>neoaurum</em> and achieved an 85.9 % molar yield of 9-OH-3-OPCM with minimal by-products. Firstly, the biosynthesis of 9-OH-3-OPCM was achieved by disrupting <em>ChsE1–2</em> and <em>ChsH1–2</em> in <em>KstDs</em>-deficient <em>M</em>. <em>neoaurum</em>. Then, key enzymes encoded by <em>ChsE4–5</em>, <em>Opccr</em>, and <em>SalA</em> were inactivated to reduce the formation of by-products 9-hydroxy-3-oxo-4,17-pregadiene-20-carboxylic acid methyl ester (9-OH-3-OPDCM) and 9,21-dihydroxy-20-methyl-pregna-4-en-3-one (9-OH-4-HBC), as well as to increase 9-OH-3-OPCM production. Notably, multiple methyltransferases were overexpressed and screened to overcome suboptimal bioconversion from precursor 9-hydroxy-3-oxo-4-pregnene-20-carboxylic acid methyl ester (9-OH-3-OPC) to 9-OH-3-OPCM, which was attributed to inadequate methyltransferase activity. Overall, our findings demonstrate a strategy to improve the efficiency and purity of 9-OH-3-OPCM biosynthesis in <em>M</em>. <em>neoaurum</em>.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"159 ","pages":"Pages 73-81"},"PeriodicalIF":4.0,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145221747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deep learning guided mutagenesis of signal peptide in Escherichia coli 深度学习引导大肠杆菌信号肽诱变

IF 4 3区生物学

Process Biochemistry Pub Date : 2025-09-25 DOI: 10.1016/j.procbio.2025.09.011

Ruei-En Hu, Hsueh-Jui Liu, Hui-Chun Chen, I-Son Ng

{"title":"Deep learning guided mutagenesis of signal peptide in Escherichia coli","authors":"Ruei-En Hu, Hsueh-Jui Liu, Hui-Chun Chen, I-Son Ng","doi":"10.1016/j.procbio.2025.09.011","DOIUrl":"10.1016/j.procbio.2025.09.011","url":null,"abstract":"<div><div>Signal peptides (SPs) play critical roles in protein secretion, yet their application in bioengineering remains limited by context-dependent functionality, host specificity, and genetic stability. This study presents a rational mutagenesis strategy for signal peptides in <em>Escherichia coli</em> under deep learning model and identifies the mutation-tolerant variants. At first, we generated 438 signal peptide sequences using SignalP 6.0. Through systematic modification of N-terminal, hydrophobic regions (H-region), and C-terminal sequence of signal peptide, we retrieved the high mutation tolerance existing in H-region. Among seven high mutation-tolerant signal peptides, SP22_54 from YjdP protein demonstrated exceptional robustness, maintaining functionality after extensive mutations. Further analysis revealed that functional SP mutants consistently preserved hydrophobic amino acid content with a minimal charge property. The SP_r1 mutant effectively mediated secretion of human carbonic anhydrase II (hCAII). The identified sequence determinants of mutation tolerance provide valuable insights for rational signal peptide design in biotechnological applications, enhancing reliability and cost-effectiveness of recombinant protein production across diverse expression systems.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"159 ","pages":"Pages 64-72"},"PeriodicalIF":4.0,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145159482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Building microcompartments using chitosan-stabilized water-in-water emulsion as templates for microbial cultivation 以壳聚糖稳定水包水乳液为模板构建微生物培养微室

IF 4 3区生物学

Process Biochemistry Pub Date : 2025-09-21 DOI: 10.1016/j.procbio.2025.09.010

Ruirui Liu , Qi Li , Huaqiang Cao , Sha Ao , Fanqiong Li , Yan Li , Bin Li , Haiqing Chen , Shilin Liu

{"title":"Building microcompartments using chitosan-stabilized water-in-water emulsion as templates for microbial cultivation","authors":"Ruirui Liu , Qi Li , Huaqiang Cao , Sha Ao , Fanqiong Li , Yan Li , Bin Li , Haiqing Chen , Shilin Liu","doi":"10.1016/j.procbio.2025.09.010","DOIUrl":"10.1016/j.procbio.2025.09.010","url":null,"abstract":"<div><div>In this study, chitosan-stabilized water in water (W/W) emulsions had been fabricated by using polyethylene oxide (PEO) and maltodextrin (MD) to encapsulate <em>Lactobacillus plantarum</em> for high-density culture. Systematic investigations had been conducted to evaluate the effects of chitosan viscosity, concentration, MRS broth concentration, ionic strength, and pH on the stability of the emulsions. The chitosan-stabilized W/W emulsions demonstrated high stability across diverse environmental conditions, exhibiting no phase separation after 7 days storage even at minimal chitosan concentrations of 0.02 wt% (weight percentage, wt%). Notably, the pH-responsive chitosan-stabilized W/W emulsions maintained structural integrity for 30 days at pH 6.0. The semi-permeable characteristics of W/W interfaces enabled efficient nutrient uptake and metabolite exchange, facilitating successful encapsulation and cultivation of <em>Lactobacillus plantarum</em> within the emulsion matrix. Optimal cultivation was achieved using emulsions containing 1.0 wt% MRS broth, yielding a peak viable cell count of 1.09 × 10<sup>9</sup> CFU/mL. These findings demonstrated that chitosan-stabilized W/W emulsions hold significant potential for probiotic protection and the development of microreactors enabling high-density microbial cultivation.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"159 ","pages":"Pages 41-50"},"PeriodicalIF":4.0,"publicationDate":"2025-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145159480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aeration influence on synergistic effects of hybrid water hyacinth and moving bed biofilm reactor in polishing low-strength synthetic wastewater 曝气对混合水葫芦与移动床生物膜反应器处理低强度合成废水协同效应的影响

IF 4 3区生物学

Process Biochemistry Pub Date : 2025-09-19 DOI: 10.1016/j.procbio.2025.09.008

Junaidah Buhari , Hassimi Abu Hasan , Siti Rozaimah Sheikh Abdullah , Seeralakandapalan Sayanthan , Masli Irwan Rosli

{"title":"Aeration influence on synergistic effects of hybrid water hyacinth and moving bed biofilm reactor in polishing low-strength synthetic wastewater","authors":"Junaidah Buhari , Hassimi Abu Hasan , Siti Rozaimah Sheikh Abdullah , Seeralakandapalan Sayanthan , Masli Irwan Rosli","doi":"10.1016/j.procbio.2025.09.008","DOIUrl":"10.1016/j.procbio.2025.09.008","url":null,"abstract":"<div><div>The hybridizing water hyacinth (WH) with a moving bed biofilm reactor (MBBR) offers a nature-based polishing strategy, in which aeration plays a key role in determining oxygen availability, biofilm stability, and pollutants removal performance. Aeration is critical part to MBBR performance, however operational guidelines for plant–biofilm hybrids remain insufficiently underdeveloped. This study investigates the effects of aeration on NH₃-N and COD removal performance that sustains the integration of macrophytes and biofilms. A two-phase laboratory scale study was conducted using WH–MBBR hybrid system to treat domestic wastewater. Phase I was operated without aeration, while Phase II was operated with continuous aeration at 2.5 L min⁻¹ with 72 h performance monitoring. The results showed that NH₃-N removal achieved 88 % at 72 h without aeration, whereas with aeration it reached 95–99 %. In addition, COD was completely removed within 28 h under aerated conditions, indicating accelerated reaction rates and improved effluent quality. Aeration is the primary factor influencing WH–MBBR polishing. Continuous low-intensity aeration enables near-complete nitrogen and organic pollutant removal. These findings provide a comparative assessment that demonstrates the role of aeration in macrophyte–MBBR performance, supporting design standards and the selection of appropriate DO range for polishing.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"159 ","pages":"Pages 33-40"},"PeriodicalIF":4.0,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145119930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of non-noble bimetallic oxides on bioelectrochemical reduction of carbon dioxide to volatile fatty acids 非贵重双金属氧化物对二氧化碳生物电化学还原为挥发性脂肪酸的影响

IF 4 3区生物学

Process Biochemistry Pub Date : 2025-09-19 DOI: 10.1016/j.procbio.2025.09.009

Tambakassi Mihin , Oumarou Savadogo , Boris Tartakovsky

{"title":"Impact of non-noble bimetallic oxides on bioelectrochemical reduction of carbon dioxide to volatile fatty acids","authors":"Tambakassi Mihin , Oumarou Savadogo , Boris Tartakovsky","doi":"10.1016/j.procbio.2025.09.009","DOIUrl":"10.1016/j.procbio.2025.09.009","url":null,"abstract":"<div><div>The conversion of CO<sub>2</sub> into value-added chemicals via microbial electrosynthesis (MES) offers a promising route for sustainable production of high-value chemicals. The study evaluates five bimetallic oxides (FeCuO, FeSnO, SnCuO, MnCuO, and CoMnO) for their impact on mixed microbial cultures capable of CO<sub>2</sub> utilization. FeSnO significantly enhanced acetogenic and chain-elongating activity while suppressing acetoclastic methanogenesis. When used to coat carbon felt cathodes in MES cells, FeSnO increased CO₂ conversion efficiency to over 90 %, and enabled the production of butyrate and caproate at rates up to 566 mg (L<sub>c</sub> d)<sup>−1</sup> and 89 mg (L<sub>c</sub> d)<sup>−1</sup>, respectively. Maximum product selectivity reached 53 % for butyrate and 10 % for caproate, surpassing by 4-fold those achieved with unmodified cathodes. Electrochemical impedance spectroscopy revealed that FeSnO coatings decreased charge transfer resistance from 209.4 Ω to 0.9 Ω, while promoting biofilm formation. These findings suggest that FeSnO coating not only enhances electrochemical performance but also facilitates microbial chain elongation critical for successful CO<sub>2</sub> conversion to medium-chain fatty acids.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"159 ","pages":"Pages 51-63"},"PeriodicalIF":4.0,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145159481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ligand recognition mechanism in genetically encoded fluorescent biosensors 基因编码荧光生物传感器中的配体识别机制

IF 4 3区生物学

Process Biochemistry Pub Date : 2025-09-17 DOI: 10.1016/j.procbio.2025.09.007

Yawen Fu, Houjin Zhang

引用次数: 0

Exploring lipids and citric acid production by Yarrowia lipolytica in lignocellulosic biomass hydrolysate-mimicking media: Effect of cultivation operation modes 探索在木质纤维素生物质水解模拟培养基中解脂耶氏菌的脂质和柠檬酸生产：培养操作模式的影响

IF 4 3区生物学

Process Biochemistry Pub Date : 2025-09-15 DOI: 10.1016/j.procbio.2025.09.006

Bruna Dias , Helena Fernandes , Isabel Belo , Marlene Lopes

{"title":"Exploring lipids and citric acid production by Yarrowia lipolytica in lignocellulosic biomass hydrolysate-mimicking media: Effect of cultivation operation modes","authors":"Bruna Dias , Helena Fernandes , Isabel Belo , Marlene Lopes","doi":"10.1016/j.procbio.2025.09.006","DOIUrl":"10.1016/j.procbio.2025.09.006","url":null,"abstract":"<div><div>This study explores the potential of two strains of <em>Yarrowia lipolytica</em>, W29 and NCYC 2904, to produce lipids-rich biomass and citric acid under batch, fed-batch, and repeated-batch cultivation in a eucalyptus bark hydrolysate-mimicking medium (EH-MM). Considering the variability in lignocellulosic hydrolysates composition, two EH-MM formulations, differing in glucose, xylose, acetic acid, formic acid, and 5-hydroxymethylfurfural concentration, were evaluated in batch mode. No growth inhibition was observed at 100 g⋅L<sup>−1</sup> glucose, but lipids accumulation was highest at 50 g⋅L<sup>−1</sup> glucose for both strains. The highest citric acid production (44 g⋅L<sup>−1</sup>) was achieved with W29 in the most concentrated medium. Fed-batch mode increased lipids content in NCYC 2904 (51 %) and citric acid in W29, whereas repeated-batch enhanced biomass production and lipids titers compared to the other strategies. The highest biomass (66 g⋅L<sup>−1</sup>) and lipids concentration (25 g⋅L<sup>−1</sup>) were obtained with NCYC 2904 in two-stage repeated-batch culture, with a C/N ratio of 10 in 1st batch and 75 in 2nd batch. The accumulated lipids were mainly composed of oleic, palmitoleic, and palmitic acids, suggesting their potential as biofuel feedstock. Overall, the results indicate that batch cultivation of W29 for citric acid and two-stage repeated-batch cultivation of NCYC 2904 for lipids are promising strategies for valorizing lignocellulose-derived sugars, underscoring the importance of strain selection and operation mode in biorefinery design.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"159 ","pages":"Pages 25-32"},"PeriodicalIF":4.0,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145098111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A hybrid XGB-ANN model integrated with Lion Optimization Algorithm for improved prediction accuracy and lactic acid yield optimization 结合Lion优化算法的混合XGB-ANN模型，提高了预测精度和乳酸产率优化

IF 4 3区生物学

Process Biochemistry Pub Date : 2025-09-09 DOI: 10.1016/j.procbio.2025.09.005

Yang Shi , Xinnan Zheng , Xiaoxia Wang , Xiangyi Shi , Cheng Hou , Shizhuo Wang , Zipeng Huang , Zheng Shen

{"title":"A hybrid XGB-ANN model integrated with Lion Optimization Algorithm for improved prediction accuracy and lactic acid yield optimization","authors":"Yang Shi , Xinnan Zheng , Xiaoxia Wang , Xiangyi Shi , Cheng Hou , Shizhuo Wang , Zipeng Huang , Zheng Shen","doi":"10.1016/j.procbio.2025.09.005","DOIUrl":"10.1016/j.procbio.2025.09.005","url":null,"abstract":"<div><div>Machine learning (ML) has been widely applied in industrial fields in recent years. However, ML faces challenges in fermentation production due to complex features and limited data. To overcome the limitations of single models and improve the prediction accuracy and stability of lactic acid fermentation, we combined six classical ML models with Artificial Neural Networks (ANN) separately in a serial manner to construct the Classical-ANN (C-ANN) hybrid models. Meanwhile, implemented the Lion Optimization Algorithm (LOA) to systematically optimize critical fermentation parameters and conducted experimental validation. While single models showed moderate performance (Extreme Gradient Boosting (XGB) R²= 0.9197; ANN R²= 0.9295), C-ANN hybrids demonstrated superior accuracy across all metrics. The XGB-ANN model achieved exceptional performance (R²=0.9850), benefiting from XGB's robust feature selection and ANN's high-dimensional processing capabilities. SHAP analysis revealed fermentation time as the most critical yield determinant, followed by total volume and pH. Lion Optimization Algorithm parameters enabled a predicted yield of 81.21 %, with experimental validation showing only 4.72 % deviation from predictions. This study not only provides valuable assistance for optimization of fermentation production conditions in the short term but also offers an innovative and effective modeling approach for future research in this field.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"159 ","pages":"Pages 1-14"},"PeriodicalIF":4.0,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145061688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of rice straw pretreatment using microwave-assisted tetrahydrofuran-water systems through response surface methodology for improved sugar recovery 利用响应面法优化微波辅助四氢呋喃-水体系预处理水稻秸秆，以提高糖的回收率

IF 4 3区生物学

Process Biochemistry Pub Date : 2025-09-09 DOI: 10.1016/j.procbio.2025.09.003

Lakshana G. Nair, Pradeep Verma

{"title":"Optimization of rice straw pretreatment using microwave-assisted tetrahydrofuran-water systems through response surface methodology for improved sugar recovery","authors":"Lakshana G. Nair, Pradeep Verma","doi":"10.1016/j.procbio.2025.09.003","DOIUrl":"10.1016/j.procbio.2025.09.003","url":null,"abstract":"<div><div>A pretreatment process is indispensable for the reduction of biomass recalcitrance to enhance its hydrolytic bioconversion efficiency. A combination of Microwave heating and Tetrahydrofuran-water co-solvent systems was applied to Rice Straw (RS) to improve its sugar conversions. The current study employed a Central Composite Design-based Response Surface Methodology (CCD-RSM) to statistically optimize and study the interaction of different independent variables, like temperature, time, substrate ratio, and enzyme loading, on the total reducing sugar (TRS) yields on the RS pretreatment. At 100 °C and 40 min with 5.0 % (w/v) substrate concentration, the THF–water pretreatment system enabled the highest sugar recovery (76.60 %) from RS, achieved using an enzyme load of 200 U/g substrate. The significance of the pretreatment model was confirmed by ANOVA, with a p-value < 0.05 and an R<sup>2</sup> value of 0.97. The crystallinity indices calculated from FTIR and XRD were incorporated into a CCD-RSM model with three factors but were found to be non-significant. However, the reduction observed in the crystallinity indices of pretreated samples, concomitant with their higher sugar yields, indicates effective amorphization of cellulose. Furthermore, the detection of 12 distinct platform chemicals in the pretreatment hydrolysates also highlights the potential sustainability of this method for downstream bioconversions.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"158 ","pages":"Pages 203-218"},"PeriodicalIF":4.0,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioremediation of Sr2+ contamination based on microbially induced carbonate precipitation (MICP) simulated by Enterobacter sp. 基于模拟微生物诱导碳酸盐沉淀（MICP）的Sr2+污染生物修复

IF 4 3区生物学

Process Biochemistry Pub Date : 2025-09-07 DOI: 10.1016/j.procbio.2025.09.002

Zhimeng Su , Ting Chen , Fuchun Li , Xinyuan Guo , Lunxian Liu , Ximin Zhang , Ming Tang , Jing Tang , Xuelin Li , Yin Yi

{"title":"Bioremediation of Sr2+ contamination based on microbially induced carbonate precipitation (MICP) simulated by Enterobacter sp.","authors":"Zhimeng Su , Ting Chen , Fuchun Li , Xinyuan Guo , Lunxian Liu , Ximin Zhang , Ming Tang , Jing Tang , Xuelin Li , Yin Yi","doi":"10.1016/j.procbio.2025.09.002","DOIUrl":"10.1016/j.procbio.2025.09.002","url":null,"abstract":"<div><div>Microbially induced carbonate precipitation (MICP) has shown significant potential for Sr<sup>2+</sup> bioremediation in both laboratory and field settings. However, previous studies have primarily focused on urease-producing bacteria, with the potential of ammonifying bacteria remaining largely uninvestigated. This study explores the capacity of the ammonifying bacterium <em>Enterobacter</em> sp. JC-1 to promote carbonate mineral formation and enhance Sr<sup>2+</sup> removal under both static and shaking conditions. Solution chemistry analysis indicated that <em>Enterobacter</em> sp. JC-1 strain elevated both the pH value and HCO<sub>3</sub><sup>-</sup> concentration, inducing supersaturation with respect to calcite and strontianite, thereby promoting carbonate precipitation. Mineralogical characterization via XRD and SEM confirmed the formation of calcium-bearing strontianite with a microspherical morphology. During mineralization, Sr<sup>2+</sup> and Ca<sup>2+</sup> co-precipitated at molar ratios of approximately 1:1 (static conditions) and < 1:1 (shaking conditions), achieving Sr<sup>2+</sup> removal rate of 22 % and 33 %. Correlation analysis identified OD<sub>600</sub> and mineral mass as key factors influencing Sr<sup>2+</sup> removal. Comparative analysis with previous studies indicated that the Sr<sup>2+</sup> removal rate of JC-1 strain was lower than that of urease-producing bacteria, it exceeded that of urease-producing fungi. These findings highlight the potential of ammonifying bacteria as viable candidates for MICP-based bioremediation of heavy metals and radionuclides, thereby expanding the applicability of MICP in environmental engineering.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"158 ","pages":"Pages 194-202"},"PeriodicalIF":4.0,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0