Process Biochemistry最新文献

Interaction between the electrochemical properties of powdered activated carbon and the biochemical processes within bacteria in Azo dye biodecolorization: An explanatory mechanism 偶氮染料生物脱色过程中粉末活性炭的电化学特性与细菌内部生化过程之间的相互作用：一种解释机制

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2024-09-26 DOI: 10.1016/j.procbio.2024.09.025

{"title":"Interaction between the electrochemical properties of powdered activated carbon and the biochemical processes within bacteria in Azo dye biodecolorization: An explanatory mechanism","authors":"","doi":"10.1016/j.procbio.2024.09.025","DOIUrl":"10.1016/j.procbio.2024.09.025","url":null,"abstract":"<div><div>Drawing on prior reports highlighting the redox mediator properties of powdered activated carbon (PAC), this study was designed to evaluate these properties to enhance the decolorization of azo dye by <em>Klebsiella quasipneumoniae</em> GT7. It was found that the presence of 0.5 % PAC in the medium increased the biodecolorization rate early in incubation. Chemical analysis revealed that dye conversion into aromatic amines occurred in microbial systems both with and without PAC. However, at initial dye concentrations (<span><math><msub><mrow><mi>C</mi></mrow><mrow><mi>id</mi></mrow></msub></math></span>) of 2 mM or higher, some dye remained on the PAC surface and in the medium. In contrast, the PAC-free system achieved nearly 100 % biodecolorization at all initial dye concentrations. The negative impact of PAC on decolorization efficiency in microbial systems with high initial dye concentrations cannot be solely explained by its redox mediator function. This study used the amphoteric-Donnan model for PAC's electrical double layer (EDL) and Mitchell's chemiosmotic model for bacterial proton motive force (PMF) to explore this. It found that charge storage in PAC's EDL regulates electron transfer fluxes, and proton species enhance the proton motive force across the bacterial membrane. These observations improve the understanding of PAC's role in microbial decolorization and its potential future applications.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142359380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antioxidant and anticancer activities on HT-29 colon cancer cells of protein isolate extracted from Cordyceps militaris fruiting body using diverse isolation methods 采用不同分离方法从冬虫夏草子实体中提取的分离蛋白对 HT-29 结肠癌细胞的抗氧化和抗癌活性

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2024-09-24 DOI: 10.1016/j.procbio.2024.09.024

{"title":"Antioxidant and anticancer activities on HT-29 colon cancer cells of protein isolate extracted from Cordyceps militaris fruiting body using diverse isolation methods","authors":"","doi":"10.1016/j.procbio.2024.09.024","DOIUrl":"10.1016/j.procbio.2024.09.024","url":null,"abstract":"<div><div>The present research examines different techniques for isolating proteins from <em>Cordyceps militaris</em> fruiting body powder (COR), namely ammonium sulfate precipitation (ASP), organic solvent precipitation (OSP), and isoelectric precipitation (IEP). ASP yielded the highest protein content (78.90 %), effectively concentrating proteins, while OSP and IEP also produced substantial yields (57.41 % and 69.06 %, respectively). Color analysis revealed differences among isolates, with ASP resulting in a darker hue. ASP and IEP showed higher red and yellow components, respectively. Essential amino acid content was significantly higher in ASP isolate compared to COR, indicating effective amino acid concentration. Thermal analysis revealed different decomposition temperatures and enthalpy values among isolates. OSP displayed the highest phenolic content, while IEP exhibited the highest flavonoid content. Antioxidant assays showed ASP to have the highest DPPH-RSA and ABTS-RSA, while IEP showed the highest FRAP value. ASP demonstrated anticancer activity against HT-29 colon cancer cells inducing cell death apoptosis and inhibiting cell migration, with an IC<sub>50</sub> of 360.63 µg/mL, comparable to Paclitaxel. These findings suggest that <em>Cordyceps militaris</em> protein isolate, particularly from ASP, has significant antioxidant and anticancer potential, supporting its potential as a natural alternative to synthetic drugs with fewer side effects.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142359379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pentagalloyl glucose enhanced the stress resistance to delay aging process in Caenorhabditis elegans 五聚酰基葡萄糖增强草履虫的抗应激能力以延缓衰老过程

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2024-09-24 DOI: 10.1016/j.procbio.2024.09.021

{"title":"Pentagalloyl glucose enhanced the stress resistance to delay aging process in Caenorhabditis elegans","authors":"","doi":"10.1016/j.procbio.2024.09.021","DOIUrl":"10.1016/j.procbio.2024.09.021","url":null,"abstract":"<div><div>Aging is a complex biological process characterized by gradual and irreversible functional deterioration, strongly associated with oxidative stress. Pentagalloyl glucose (PGG) has attracted increasing attention due to its potent antioxidant and anti-stress properties. This study investigated the potential of PGG to mitigate the aging process under stress in RAW 264.7 cells and <em>Caenorhabditis elegans</em> models. The expression of vital genes associated with stress was also measured to explain the action mechanism of PGG in <em>C. elegans</em>. The findings showed that PGG supplementation not only significantly enhanced the stress tolerance of RAW 264.7 cells, but also prolonged lifespan and reduced the ROS and lipofuscin accumulation in <em>C. elegans</em> induced by stress. Meanwhile, the improvement effect of PGG on delaying aging development was also manifested in the protection of mitochondrial function and neuronal integrity. Moreover, <em>daf-16</em> nuclear translocation and <em>sod-3</em> expression were significantly enhanced by PGG to delay the aging process. Mechanistically, PGG might alleviate aging by improving <em>daf-16</em>, <em>sod-3</em>, <em>ctl-1</em>, and <em>gst-4</em> levels in the DAF-16/FOXO pathway and upregulating <em>skn-1</em> and <em>gst-4</em> expression in the SKN-1/Nrf2 pathway. Our study provided novel insights into the role of PGG in combating stress-induced aging.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142327943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insight into microorganisms and flavor substances in traditional Chinese fermented food starter: Daqu 洞察中国传统发酵食品开胃菜中的微生物和风味物质：大曲

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2024-09-24 DOI: 10.1016/j.procbio.2024.09.015

{"title":"Insight into microorganisms and flavor substances in traditional Chinese fermented food starter: Daqu","authors":"","doi":"10.1016/j.procbio.2024.09.015","DOIUrl":"10.1016/j.procbio.2024.09.015","url":null,"abstract":"<div><div>Daqu, a crucial fermentation starter in the production of various Chinese fermented foods, plays a pivotal role in shaping complex enzyme profiles and diverse flavour precursors. This review aims to elucidate the microbial communities within Daqu, focusing on their functionalities in the context of flavour development. We delve into the detection methods of microorganisms and flavour substances in Daqu, employing advanced technologies including high-performance liquid chromatography, gas chromatography, pseudo-targeted metabolomics, and headspace solid-phase microextraction-gas chromatography-mass spectrometry. This review explores high throughput sequencing technologies, including pyrosequencing, clonal library sequencing, metaproteomic, and metagenomics, to gain a comprehensive understanding of the intricate microbial dynamics. Additionally, we discuss the metabolic pathways involved in flavour substance production within Daqu. By synthesizing information on Daqu types, microorganisms present, detection methodologies, and flavour substance metabolic pathways, this review contributes to a deeper comprehension of the intricate processes underpinning the Flavors of Chinese fermented foods.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142322063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding Bacillus response to salt stress: Growth inhibition, enhanced EPS secretion, and molecular adaptation mechanisms 了解芽孢杆菌对盐胁迫的反应：生长抑制、EPS 分泌增强和分子适应机制

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2024-09-24 DOI: 10.1016/j.procbio.2024.09.023

{"title":"Understanding Bacillus response to salt stress: Growth inhibition, enhanced EPS secretion, and molecular adaptation mechanisms","authors":"","doi":"10.1016/j.procbio.2024.09.023","DOIUrl":"10.1016/j.procbio.2024.09.023","url":null,"abstract":"<div><div>This study investigates the secretion pattern of extracellular polymeric substances (EPS) by <em>Bacillus</em> sp. under varying salt concentrations and elucidates the molecular mechanisms governing EPS synthesis and secretion. Salt stress inhibited cell proliferation, while optimal salt stimulation promoted EPS secretion, resulting in increased viscosity of the culture medium and the formation of bacterial clusters. Fourier infrared spectrum analysis revealed functional groups such as C-O-C and N-H within the EPS. Soluble-EPS (S-EPS) contained sulfur and phosphorus groups associated with heavy metal ions adsorption. The study also identified a novel polysaccharide formed through bonding EPS (B-EPS). High salt concentrations correlated with elevated levels of tryptophan protein and its derivatives, increased tyrosine polysaccharide derivatives, and decreased aromatic polysaccharides. B-EPS exhibited higher levels of aromatic polysaccharides, with Na<sup>+</sup> promoting detachment of B-EPS from the cell surface. Transcriptome sequencing (RNA-seq) analysis under salt stress revealed significant expression of spore kinase (<em>Kin</em>D) and response regulatory protein <em>Spo0</em>A in the phosphoric acid relay system. Key transcriptional regulatory factors, including <em>Omp</em>R and exopolysaccharide biosynthesis, were closely associated with EPS synthesis and secretion. This study establishes a theoretical foundation for the industrial production and practical application of EPS by elucidating the molecular mechanisms underlying <em>Bacillus</em>' response to salt stress.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142316249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation of a novel laccase-modified Fe3O4/TiO2 catalyst for simultaneous delignification and saccharification of Spartina alterniflora Loisel 制备新型漆酶改性的 Fe3O4/TiO2 催化剂，用于同时进行交替叶斯巴达Loisel的脱木质和糖化处理

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2024-09-22 DOI: 10.1016/j.procbio.2024.09.019

{"title":"Preparation of a novel laccase-modified Fe3O4/TiO2 catalyst for simultaneous delignification and saccharification of Spartina alterniflora Loisel","authors":"","doi":"10.1016/j.procbio.2024.09.019","DOIUrl":"10.1016/j.procbio.2024.09.019","url":null,"abstract":"<div><div>The development of a cost-effective and environment-friendly conversion method to overcome the recalcitrance of biomass is a challenging task for enhancing the value-added potential of lignocellulosic compounds. In this study, a photo-enzyme composite catalyst by immobilizing laccase on Fe<sub>3</sub>O<sub>4</sub>/TiO<sub>2</sub> (denoted as LC-Fe<sub>3</sub>O<sub>4</sub>/TiO<sub>2</sub>) was applied to the pretreatment and enzymatic saccharification of <em>Spartina alterniflora Loisel</em> (SAL) under the action of alternating light/dark cycles. The effect of the pretreatment temperature, pretreatment time, LC-Fe<sub>3</sub>O<sub>4</sub>/TiO<sub>2</sub> concentration, mass concentration of straw particles, and hydrolysis time on delignification efficiency and reducing sugar yield was investigated by a central composite design (CCD). Under the selected conditions (48 ℃, pretreatment time of 70 min, LC-Fe<sub>3</sub>O<sub>4</sub>/TiO<sub>2</sub> concentration of 4.9 mg/mL, mass concentration of straw particles of 22 % (w/v), hydrolysis time of 55 h), 92.53 % delignification and 129.85 mg/g reducing sugar yield were achieved. The enzymatic hydrolysis efficiency was higher compared to conventional methods. Moreover, the LC-Fe<sub>3</sub>O<sub>4</sub>/TiO<sub>2</sub> showed good recyclability and reusability. Developed LC-Fe<sub>3</sub>O<sub>4</sub>/TiO<sub>2</sub> particles have a high potential for use in biomass utilization.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142311427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beta-glucan: An overview in biological activities, derivatives, properties, modifications and current advancements in food, health and industrial applications β-葡聚糖：概述生物活性、衍生物、特性、改性以及在食品、健康和工业应用方面的最新进展

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2024-09-21 DOI: 10.1016/j.procbio.2024.09.011

{"title":"Beta-glucan: An overview in biological activities, derivatives, properties, modifications and current advancements in food, health and industrial applications","authors":"","doi":"10.1016/j.procbio.2024.09.011","DOIUrl":"10.1016/j.procbio.2024.09.011","url":null,"abstract":"<div><div>β-glucans have garnered significant attention due to their numerous health-promoting and prebiotic attributes. β-glucans, a group of bioactive substance, are known to have biological effects such as immune-modulating, anti-inflammatory, anti-cancer, anti-senescence, hypoglycemic and hypocholesterolemic properties. Owing to its unique physical characteristics including its solubility, viscosity and gelation, the food industry and other sectors have witnessed a rise in the utilization of β-glucan. This study aims to present an overview of β-glucan manufacturing technologies, including extraction, isolation, and purification methods from different sources, comprising of bacteria, fungi and plants such as cereals, with the intention of enhancing these techniques for more productive manufacturing procedures. Additionally, β-glucans' physical characteristics, chemical alterations, prospective industrial uses, and future application prospects in food, pharmaceuticals, cosmetics, and other potentially value-added product are outlined research suggest that β-glucans will become more significant in the global food and health sectors in the future.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142311147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extraction methods of dietary fiber and effect on bioactivity: A review 膳食纤维的提取方法及其对生物活性的影响：综述

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2024-09-20 DOI: 10.1016/j.procbio.2024.09.017

{"title":"Extraction methods of dietary fiber and effect on bioactivity: A review","authors":"","doi":"10.1016/j.procbio.2024.09.017","DOIUrl":"10.1016/j.procbio.2024.09.017","url":null,"abstract":"<div><div>A large number of food processing by-products are consumed every day that are not utilized and are rich in highly active nutrients: dietary fiber (DF). DF has been defined by nutritionists as the seventh macronutrient with many benefits to our health, such as improving intestinal flora, reducing obesity and lowering cardiovascular morbidity. Thus, suitable extraction methods are needed to obtain high yields of high and quality DFs (including the soluble dietary fiber and insoluble dietary fiber). DF extraction methods have received increasing attention because it is effective in improving the structure and functional properties of DF. However, to the best of our knowledge, no study has systematically analyzed and summarized the differences in different DF extraction methods and their exert on DF composition, structure and functional activities. In this paper, the extraction methods (physical method, chemical method, biological method and combined method) for DF which have been explored by researchers in the last decade are reviewed, and the effects caused by different extraction methods on the structure and functional activity of DF from different sources are summarized. Moreover, the mechanism, comparative analysis and future trends of different extraction methods for DF are discussed. This review will greatly promote the development and utilization of plant and by-product resources in China, and provide a theoretical basis for the demand of high-quality DFs for our people. Meanwhile, this review can provide theoretical guidance for selecting suitable DF extraction methods and achieving better industrialization of extracted DF.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142322064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of composite microbial agent with straw or sawdust on organic matter degradation, enzyme activity and functional genes during aerobic composting of food waste 秸秆或锯末复合微生物制剂对厨余垃圾好氧堆肥过程中有机物降解、酶活性和功能基因的影响

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2024-09-20 DOI: 10.1016/j.procbio.2024.09.018

{"title":"Effects of composite microbial agent with straw or sawdust on organic matter degradation, enzyme activity and functional genes during aerobic composting of food waste","authors":"","doi":"10.1016/j.procbio.2024.09.018","DOIUrl":"10.1016/j.procbio.2024.09.018","url":null,"abstract":"<div><div>Bulking agent and microbial agent are widely used to improving the aerobic composting condition. This study aimed to evaluate the effects of composite microbial agent which is consisted of <em>Azotobacter salinestris, Aspergillus oryzae, Bacillus licheniformis, Bacillus cereus</em> and <em>Trichoderma viride</em> with different bulking agents (straw or sawdust) on organic matter degradation, enzyme activities, and functional genes associated with carbon and nitrogen cycling during aerobic composting of food waste. The results demonstrated that the inoculation of composite microbial agent significantly improved the degradation of starch, protein, cellulose, and grease, along with boosting microbial enzyme activity and the abundance of carbon degradation genes. Furthermore, the degradation of organic matter (OM), protein, cellulose, and grease were higher in the straw bulked composting with composite microbial agent treatment (B). Meanwhile, B also exhibited a higher abundance of <em>cdh</em> and <em>rbcL</em> genes compared to the other treatments. Pearson regression analysis identified temperature as the primary factor controlling organic matter degradation, while <em>cdh</em> and <em>cex</em> genes exhibited strong associations with cellulose degradation during food waste composting. These findings suggest that incorporating straw as bulking agent with composite microbial agent during food waste composting can effectively enhance organic matter degradation.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142318958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced enzymatic hydrolysis of corn stover by γ-valerolactone pretreatment and the characteristics of enzymatic residues γ-戊内酯预处理对玉米秸秆酶水解的促进作用及酶残留物的特征

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2024-09-19 DOI: 10.1016/j.procbio.2024.09.016

{"title":"Enhanced enzymatic hydrolysis of corn stover by γ-valerolactone pretreatment and the characteristics of enzymatic residues","authors":"","doi":"10.1016/j.procbio.2024.09.016","DOIUrl":"10.1016/j.procbio.2024.09.016","url":null,"abstract":"<div><p>Efficient pretreatment methods are crucial for the generation of fermentable sugars from the renewable lignocellulose. In this study, γ-valerolactone (GVL), a biomass-derived green solvent, was used to fractionate corn stover (CS). The impact of different additives on the enzymatic hydrolysis and pretreatment of CS within the GVL/water system was carefully investigated. The results demonstrate that the use of H<sub>2</sub>SO<sub>4</sub>-assisted GVL/H<sub>2</sub>O (80:20, w/w) pretreatment at mild conditions successfully facilitates the depolymerization of CS, thereby improving the cellulase accessibility. Furthermore, by increasing the GVL pretreatment temperature to 160 °C with 100 mM H<sub>2</sub>SO<sub>4</sub> addition, the pretreated CS led to nearly 100 % of hydrolysis yield at 2 % (w/v) solid load. Finally, the thermogravimetric analysis (TGA) was performed to evaluate the thermal decomposition characteristics of untreated CS and enzymatic residues based on the H<sub>2</sub>SO<sub>4</sub>-assisted GVL/H<sub>2</sub>O pretreatment. The results indicate that the enzymatic residues had two depolymerization stages from 200 °C to 500 °C with a final residual mass of 22.73 %, which was higher than that of untreated CS (20.56 %). Overall, this study provides an efficient lignocellulose pretreatment for fermentable sugars production and valorization of enzymatic residues.</p></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142274614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0