Process Biochemistry最新文献

Temperature fluctuations drove the interactions dynamic of keystone microbes for Chinese baijiu fermentation 温度波动驱动白酒发酵关键微生物相互作用动态

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-07-21 DOI: 10.1016/j.procbio.2025.07.016

Zijian Miao , Xinlei Wang , Wenjing Wang , Bowen Wang , Jinyuan Sun , Zexia Li , Fuping Zheng , Yuhang Zhang , Baoguo Sun

{"title":"Temperature fluctuations drove the interactions dynamic of keystone microbes for Chinese baijiu fermentation","authors":"Zijian Miao , Xinlei Wang , Wenjing Wang , Bowen Wang , Jinyuan Sun , Zexia Li , Fuping Zheng , Yuhang Zhang , Baoguo Sun","doi":"10.1016/j.procbio.2025.07.016","DOIUrl":"10.1016/j.procbio.2025.07.016","url":null,"abstract":"<div><div>Temperature is an important abiotic factor to drive the spontaneous food fermentations, however, the effects of temperature on these processes still remain unclear. In this study, we revealed the dynamics of temperature, microbiota and metabolites in the typical Chinese baijiu fermentation. Moreover, we established a specific data-driven correlation analysis model based on machine learning. We revealed 13 high-abundance and 6 low-abundance species as the keystone microbes in this fermentation, by overall considering abundance, function and frequency of fermented microorganisms. In addition, the increasing of heating rate (from 2 to 5 ℃/day) strengthened the bacterial-bacterial interactions and weakened the bacterial-fungal interactions among these keystone microbes, subsequently affecting the metabolite shifts in baijiu fermentation. Therefore, we proposed a data-driven keystone microbe’s identification framework, and revealed the effects of temperature fluctuation on the interaction and metabolism of keystone microbes. Moreover, this study would pave the way for the identification of keystone microbes within complex microbial communities, and guide the optimization of fermentation processes for improving the quality of fermented products.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 197-206"},"PeriodicalIF":3.7,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144695201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enzyme supplementation as a strategy for enhancing the cellulose and hemicellulose conversion yields at high solids content of pretreated rice straw 在高固含量条件下，酶补充对提高预处理稻秆纤维素和半纤维素转化率的影响

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-07-21 DOI: 10.1016/j.procbio.2025.07.013

Giovanni H. Silva, Miguel A.D. Flores-Alarcón, Lina M. Durán, Inês C. Roberto

{"title":"Enzyme supplementation as a strategy for enhancing the cellulose and hemicellulose conversion yields at high solids content of pretreated rice straw","authors":"Giovanni H. Silva, Miguel A.D. Flores-Alarcón, Lina M. Durán, Inês C. Roberto","doi":"10.1016/j.procbio.2025.07.013","DOIUrl":"10.1016/j.procbio.2025.07.013","url":null,"abstract":"<div><div>An efficient strategy for the high-solids enzymatic hydrolysis of lignocellulosic biomass is crucial for producing a sugar-rich hydrolysate. In this sense, the effect of Viscozyme L supplementation at different loads (5 – 60 mg protein/g hemicellulose) of Cellic CTec2 on cellulose (GCY) and hemicellulose conversion yield (HCY) was evaluated, using 20 % w/v of pretreated rice straw in shake flasks. At optimum Viscozyme L load (15 mg protein/g hemicellulose), 87 and 66 % of GCY and HCY were achieved, respectively. Then, the release of sugars was further improved by using an anchor-type impeller reactor. The resulting hydrolysate, composed of (g/L): glucose (∼85), xylose (∼38), and arabinose (∼8), was finally fermented by <em>Candida guilliermondii</em> FTI 20037 yeast, showing high efficiency (96 %) and volumetric productivity (2.06 g/L/h) from glucose. Therefore, supplementation with commercially available enzymes in a non-conventional reactor improved the performance of high-solids enzymatic hydrolysis and could be a promising strategy for industrial applications.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 207-215"},"PeriodicalIF":3.7,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144695202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Small yellow ginger extract: Preparation, characterization, antioxidant properties, and protective activities against alcohol-induced HepG2 cell injury 小黄姜提取物的制备、表征、抗氧化性能和对酒精诱导的HepG2细胞损伤的保护作用

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-07-17 DOI: 10.1016/j.procbio.2025.07.011

Qiubian Yang , Peihang Chen , Ruimin Zhong , Jianyin Miao

{"title":"Small yellow ginger extract: Preparation, characterization, antioxidant properties, and protective activities against alcohol-induced HepG2 cell injury","authors":"Qiubian Yang , Peihang Chen , Ruimin Zhong , Jianyin Miao","doi":"10.1016/j.procbio.2025.07.011","DOIUrl":"10.1016/j.procbio.2025.07.011","url":null,"abstract":"<div><div>Small yellow ginger (<em>Zingiber officinale Roscoe</em>) is a traditional medicinal and edible plant. In this study, response surface methodology was used to optimize the preparation process of small yellow ginger extract using total polyphenols content as an indicator, and the chemical composition, antioxidant capacity and protective effects of the extract on alcohol-induced injury in HepG2 cells were investigated. The results implied that the best conditions to reach the highest value of total polyphenols content (10.59 mg GAE/g) were an extraction time of 32 min, ethanol concentration of 71 % v/v, solid-liquid ratio of 1:19 g/mL, and temperature of 60 ℃. The 67 main components were identified by UPLC-MS/MS. The extract exhibited excellent DPPH and ABTS radical scavenging effects (IC<sub>50</sub> of 0.11 mg/mL and 28.83 μg/mL, respectively) and ORAC values (758.36 μmol TE/g lyophilized powder). The CAA assay indicated that the extract possessed potent antioxidant activity (46.22 μg/mL) against HepG2 cells. Furthermore, the extract exhibited a significant protective effect on ethanol-induced HepG2 cell injury. This study provides a scientific basis for the development of functional foods and natural antioxidants based on small yellow ginger and promotes its application in the prevention and treatment of alcoholic liver disease.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 183-196"},"PeriodicalIF":3.7,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144695203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fermentation-assisted enzymatic hydrolysis improves the antioxidant activity of egg white peptides 发酵辅助酶解提高了蛋清肽的抗氧化活性

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-07-17 DOI: 10.1016/j.procbio.2025.07.010

Lihui Zhang , Feng Zang , Jinyan Gao , Hongbing Chen , Ping Tong

引用次数: 0

Microalgae-based nutritional supplements: Sustainable applications for high-nutritional-value food production 基于微藻的营养补充剂：高营养价值食品生产的可持续应用

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-07-16 DOI: 10.1016/j.procbio.2025.07.009

Ricky Rajamanickam , Satwika Das , Chandukishore T , Shagun Sharma , Rajesh R O , Ashish A. Prabhu , Sanjukta Banerjee , Nur Izyan Wan Azelee , Sankaran Krishnamoorthy , Rangabhashiyam Selvasembian

{"title":"Microalgae-based nutritional supplements: Sustainable applications for high-nutritional-value food production","authors":"Ricky Rajamanickam , Satwika Das , Chandukishore T , Shagun Sharma , Rajesh R O , Ashish A. Prabhu , Sanjukta Banerjee , Nur Izyan Wan Azelee , Sankaran Krishnamoorthy , Rangabhashiyam Selvasembian","doi":"10.1016/j.procbio.2025.07.009","DOIUrl":"10.1016/j.procbio.2025.07.009","url":null,"abstract":"<div><div>Microalgae, photosynthetic organisms that flourish in many aquatic habitats and are rich in vital nutrients, making them a valuable resource for humans and animals, have gained growing interest for their potential to transform into food production. Despite their microscopic size, microalgae are packed with essential nutrients like protein, vitamins, and antioxidants, offering a concentrated source of nutrition. This review aims to explore the nutritional, functional, and commercial potential of microalgae in food applications, focusing on their role in the development of food supplements and novel food formulations. Over recent decades, microalgae have been steadily introduced into the food industry and have seen modest expansion. Due to their high content of beneficial fatty acids (linoleic acid, gamma-linolenic acid, and arachidonic acid), carotenoids, vitamins, phycobilin pigments, highly digestible proteins, lipids, and carbohydrates, microalgae are becoming recognised for their sustainable valorization potential. This review highlights key microalgal species generally recognized as safe (GRAS) and their integration into functional foods. It also discusses emerging trends and biotechnological advancements in microalgae-based food products, underscoring their potential to address global nutritional and sustainability challenges.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 162-182"},"PeriodicalIF":3.7,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144678838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “Progress in chitosan-based materials: Enhancing edible coatings and films through modifications and functionalization for food preservation” [Process Biochem. 156 (2025) 175–190] “壳聚糖基材料的进展：通过改性和功能化增强可食用涂层和薄膜用于食品保存”的勘误表[过程生物化学，156 (2025)175-190]

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-07-15 DOI: 10.1016/j.procbio.2025.07.005

Maricarmen Iñiguez-Moreno , Josué David Hernández-Varela , Manuel Burelo , Joel H. Elizondo-Luevano , Rafael G. Araújo , Cecilia D. Treviño-Quintanilla , Dora I. Medina

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Pea protein isolate and sour cherry polyphenols interaction improved the stability of polyphenols and the efficacy in alleviating colitis 豌豆分离蛋白与酸樱桃多酚相互作用提高了多酚的稳定性和缓解结肠炎的疗效

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-07-12 DOI: 10.1016/j.procbio.2025.07.008

Guihua Wang, Bingjie Liu, Xianghong Meng

{"title":"Pea protein isolate and sour cherry polyphenols interaction improved the stability of polyphenols and the efficacy in alleviating colitis","authors":"Guihua Wang, Bingjie Liu, Xianghong Meng","doi":"10.1016/j.procbio.2025.07.008","DOIUrl":"10.1016/j.procbio.2025.07.008","url":null,"abstract":"<div><div>To enhance the bioavailability of sour cherry polyphenol extract (SCE), pea protein isolate (PPI) with low allergenicity was selected to form the complex with SCE. The storage and digestion characteristics of the complex, along with its effects on relieving colitis were evaluated. The results showed that after complexing with PPI, the storage stability of SCE was improved. After digestion, the PSA group (PPI-SCE complex prepared at pH 3) delivered more polyphenols to the small intestine. PSA was more effective in alleviating ulcerative colitis compared with SCE alone, which inhibited the excessive activation of the TLR4/NF-κB pathway. The shortening of the colon length was mitigated. It was manifested in a reduction in inflammatory factor levels, alleviation of colonic damage, and less tissue structure destruction. Meanwhile, PSA upregulated the gene expression of tight-junction proteins such as zonula occludens-1, occludin, and claudin-4, enhancing the integrity of the intestinal barrier and thus alleviating the symptoms of ulcerative colitis. This study provided a reference for the high-value utilization of SCE and the extensive application of PPI.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 112-121"},"PeriodicalIF":3.7,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144654916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasound-enhanced delivery of pterostilbene-loaded long-circulating solid lipid nanoparticles for the inhibition of IL-6/STAT3 signaling pathway in ovarian cancer 超声增强的紫芪载长循环固体脂质纳米颗粒对卵巢癌中IL-6/STAT3信号通路的抑制作用

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-07-09 DOI: 10.1016/j.procbio.2025.06.017

Wenjing Wang, Haibin Xi, Yi Ping

{"title":"Ultrasound-enhanced delivery of pterostilbene-loaded long-circulating solid lipid nanoparticles for the inhibition of IL-6/STAT3 signaling pathway in ovarian cancer","authors":"Wenjing Wang, Haibin Xi, Yi Ping","doi":"10.1016/j.procbio.2025.06.017","DOIUrl":"10.1016/j.procbio.2025.06.017","url":null,"abstract":"<div><div>The current investigation explores a novel combinational approach for ovarian cancer treatment by formulating Pterostilbene-loaded solid lipid nanoparticles (PT-SLNPs) and enhancing their therapeutic efficiency using ultrasound stimulation. The formulated PT-SLNPs displays favorable physicochemical properties, with a mean particle size of 133.46 ± 24.21 nm, a low polydispersity index (0.14 ± 0.02), and high entrapment efficiency (87.76 ± 0.5 %). The uniqueness of this work is attributed to the synergistic application of PT-SLNPs and ultrasound (US), which significantly enhanced the cytotoxic effect against human ovarian cancer cell A2780. Further, flowcytometry method confirmed a considerable increase in apoptosis in cells treated with PT-SLNPs + US combination. Mechanistically, exposure of cancer cells to PT-SLNPs + US downregulated the key tumor associated pro-survival and proliferative pathways by suppressing IL-6 expression and inhibiting STAT3 phosphorylation. These findings highlight the innovative potential of US assisted PT-SLNPs as a promising, less toxic alternative to conventional chemotherapy and radiotherapy in ovarian cancer treatment.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 122-135"},"PeriodicalIF":3.7,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144654917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiplex metabolic engineering for enhanced indole-3-acetic acid production via optimized biosynthetic pathways in E. coli 通过优化大肠杆菌生物合成途径提高吲哚-3-乙酸生产的多重代谢工程

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-07-09 DOI: 10.1016/j.procbio.2025.07.006

Fabien Nsanzabera , Binbin Liu

{"title":"Multiplex metabolic engineering for enhanced indole-3-acetic acid production via optimized biosynthetic pathways in E. coli","authors":"Fabien Nsanzabera , Binbin Liu","doi":"10.1016/j.procbio.2025.07.006","DOIUrl":"10.1016/j.procbio.2025.07.006","url":null,"abstract":"<div><div>IAA is a key auxin that regulates plant growth and development. Engineering <em>E. coli</em> as a microbial cell factory provides a sustainable alternative to petrochemical-based production methods by using glucose as a feedstock. This review outlines fundamental strategies that enhance microbial IAA synthesis while simplifying technical concepts for broader understanding. Key approaches include overexpressing genes in the shikimate pathway and modifying central carbon metabolism (PP and EMP pathways) to increase precursor availability, particularly E4P and PEP. Enhancing cofactor supply, through improved NAD(P)H and ATP regeneration and FMN and FAD preservation, supports the energy and redox demands of IAA biosynthesis. Enhancing intracellular L-tryptophan levels by boosting import and limiting efflux further contributes to IAA productivity. Balancing growth with metabolite output ensures process efficiency. The review highlights forward-looking strategies, including machine learning for pathway design, genome-scale modeling to identify bottlenecks, and the use of modular or co-culture engineering to optimize microbial IAA biosynthesis. It underscores the potential of engineered <em>E. coli</em> as a sustainable platform for IAA production, paving the way for greener agricultural practices and reduced reliance on petrochemicals.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 147-161"},"PeriodicalIF":3.7,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144654915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic engineering of Yarrowia lipolytica to produce menaquinone-7 溶脂耶氏菌代谢工程生产甲基萘醌-7

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-07-09 DOI: 10.1016/j.procbio.2025.07.007

Yeqiao Shen , Xuezhen Wang , Shoubo Zuo , Zhongmin Tang , Zhengjun Xiong , Jie Xiong , Huili Zhang

{"title":"Metabolic engineering of Yarrowia lipolytica to produce menaquinone-7","authors":"Yeqiao Shen , Xuezhen Wang , Shoubo Zuo , Zhongmin Tang , Zhengjun Xiong , Jie Xiong , Huili Zhang","doi":"10.1016/j.procbio.2025.07.007","DOIUrl":"10.1016/j.procbio.2025.07.007","url":null,"abstract":"<div><div>Menaquinone-7 (MK-7), a vital subtype of fat-soluble vitamin K2, plays essential physiological roles in preventing osteoporosis, vascular calcification, and reducing vascular damage. Its broad applications in functional foods and pharmaceuticals have attracted significant attention. <em>Yarrowia lipolytica</em>, with its abundant acetyl-CoA supply and functional mevalonate (MVA) pathway for synthesizing the polyprenyl side chain of MK-7, demonstrates exceptional capabilities in transporting and storing hydrophobic compounds, making it an ideal host for MK-7 production. In this study, we developed an engineered strain of <em>Y. lipolytica</em> to achieve high-titer production of MK-7 through supplementation with exogenous precursors, 1,4-dihydroxy-2-naphthoic acid (DHNA) or menadione (VK3). By employing metabolic engineering, peroxisome engineering, and fermentation optimization, the final strain YQ-9 produced 255 ± 0.58 mg/L MK-7 after 144 h of shake-flask fermentation, representing the highest reported titer in <em>Y. lipolytica</em> to date. This study not only lays the foundation for the future de novo MK-7 synthesis in <em>Y. lipolytica</em> but also provides novel insights for industrial-scale MK-7 production<em>.</em></div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 101-110"},"PeriodicalIF":3.7,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144604886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0