Process Biochemistry最新文献

A Novel Integrated Alginate-Immobilized Biosystem with Pulse Feeding and Cell Recycling for Semi-Continuous Production of the Immunosuppressant Mycophenolic Acid by Penicillium roqueforti 一种新型海藻酸固定化脉冲进料和细胞循环的半连续生产免疫抑制剂霉酚酸的生物系统

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-05-07 DOI: 10.1016/j.procbio.2026.05.002

El-Sayed R. El-Sayed , Mohamed A. Taha , Aliaa A. Elshamy , Gharieb S. El-Sayyad

{"title":"A Novel Integrated Alginate-Immobilized Biosystem with Pulse Feeding and Cell Recycling for Semi-Continuous Production of the Immunosuppressant Mycophenolic Acid by Penicillium roqueforti","authors":"El-Sayed R. El-Sayed , Mohamed A. Taha , Aliaa A. Elshamy , Gharieb S. El-Sayyad","doi":"10.1016/j.procbio.2026.05.002","DOIUrl":"10.1016/j.procbio.2026.05.002","url":null,"abstract":"<div><div>Mycophenolic acid is a valuable fungal metabolite with major pharmaceutical applications. In the present study, <em>Penicillium roqueforti</em> GM1013, was used to enhance mycophenolic acid production through immobilization and feeding strategies. The fungus was immobilized by entrapment of fresh mycelium in calcium alginate beads, and key immobilization parameters were optimized using response surface methodology. Under the optimized immobilization conditions, the predicted maximum mycophenolic acid concentration closely matched the experimental value 3.25 ± 0.76gL⁻¹, confirming the accuracy of the model. In addition, fed-up strategies of Tween 80, glycine, and methionine were studied using immobilized cultures in shake-flask fermentation for 10 days. The four-pulse feeding strategy (Day 2 to Day 5) produced the highest mycophenolic acid titer (5.87 ± 0.92gL⁻¹) and the highest productivity compared with batch addition and other feeding schedules. Delayed four-pulse feeding (Day 3 to Day 6) also improved production but resulted in a lower final titer (4.92 ± 0.67gL⁻¹). Finally, semi-continuous production using bead recycling confirmed that immobilized cultures could be reused for multiple cycles, achieving a total mycophenolic acid production of 30.64gL⁻¹ over eight cycles, although productivity decreased gradually due to culture aging. Overall, the study demonstrates that combining immobilization optimization with controlled fed-up supplementation is an effective approach for improving mycophenolic acid production and process efficiency.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"167 ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147855096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combinatorial optimization of cellular regulation and fermentation for high-efficiency expression of cephalosporin C acylase in Escherichia coli 大肠杆菌中高效表达头孢菌素C酰化酶的细胞调控与发酵组合优化

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-04-01 Epub Date: 2026-02-02 DOI: 10.1016/j.procbio.2026.02.003

Xuyang Huang , Miaomiao Xia , Huina Dong , Xinyi Ren , Gang Fu , Bo Yuan , Aitong Jiang , Long Chen , Zhoutong Sun , Siriguleng Qian , Dawei Zhang

{"title":"Combinatorial optimization of cellular regulation and fermentation for high-efficiency expression of cephalosporin C acylase in Escherichia coli","authors":"Xuyang Huang , Miaomiao Xia , Huina Dong , Xinyi Ren , Gang Fu , Bo Yuan , Aitong Jiang , Long Chen , Zhoutong Sun , Siriguleng Qian , Dawei Zhang","doi":"10.1016/j.procbio.2026.02.003","DOIUrl":"10.1016/j.procbio.2026.02.003","url":null,"abstract":"<div><div>Cephalosporin antibiotics are among the most widely used β-lactam antibiotics in clinical practice, and their industrial production is a critical focus in the pharmaceutical field. The cephalosporin C acylase mutant A14 from <em>Bosea sp</em>. OK403 identified in our previous work exhibited low protein expression levels. In this study, we evaluated three strategies: rare codon optimization, fusion tag addition, and co-expression with molecular chaperone, to improve the expression of A14. Rare codon optimization had little effect on A14 expression. Six fusion tags (NusA, SUMO, GST, MBP, DsbC, TrxA) and ten molecular chaperone plasmids (pG-KJE8, pGro7, pKJE7, pG-Tf2, pTf16, PpiD, PpiA, SurA, FkpA, Skp) were evaluated. Both NusA fusion tag and pGro7 chaperone plasmid effectively enhance A14 expression. The highest A14 expression was observed in strain E-N + p, which co-expressed NusA and pGro7. Additionally, We optimized the fermentation of strain E-A14 in a 5 L bioreactor by adjusting parameters, media, and inducer addition timing. Strain E-N + p showed a 1.7-fold increase in protein titer (7.91 g/L) and a 2.1-fold increase in enzyme activity (340 U/L) compared to the original strain. This study provides an effective strategy for optimizing the production of cephalosporin C acylase and offers valuable insights for industrial-scale production of β-lactam antibiotics.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"163 ","pages":"Pages 16-26"},"PeriodicalIF":4.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146102595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pomegranate waste valorization: A novel approach to eco-friendly soap formulation with enhanced germicidal properties 石榴废料增值：一种新型的环保肥皂配方，具有增强的杀菌性能

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-04-01 Epub Date: 2026-02-09 DOI: 10.1016/j.procbio.2026.02.006

Dikshita Aneja , Shantam Gautam , Pratiksha Gondkar , Mousumi Debnath

{"title":"Pomegranate waste valorization: A novel approach to eco-friendly soap formulation with enhanced germicidal properties","authors":"Dikshita Aneja , Shantam Gautam , Pratiksha Gondkar , Mousumi Debnath","doi":"10.1016/j.procbio.2026.02.006","DOIUrl":"10.1016/j.procbio.2026.02.006","url":null,"abstract":"<div><div>The study focuses on valorizing pomegranate peel and leaves as agro-waste to develop eco-friendly soap formulations. Pomegranate peel showed ∼2–3 fold higher phenolics, flavonoids, and antioxidants as compared to leaves. Green synthesized zinc oxide nanoparticles were characterized and incorporated into soap formulations, which exhibited 89–90 % total fatty matter, neutral pH, and a moisture content of 12–14 %. The optimized Grade 1 peel-based and leaf-based nano herbal soap formulations showed emulsification indices of 61.03 % and 57.14 %, respectively. The formulations showed antimicrobial activity against <em>B. subtilis</em>, <em>P. aeruginosa,</em> and <em>S. aureus</em>. The common metabolites present in both pomegranate peel and leaves were subjected to drug docking and ADME analysis. Docking studies identified punicalagin as a potential anti-inflammatory compound with the maximum binding affinity against COX-2 and TNF-α. In contrast, ADME analysis showed that ellagic acid has the highest skin permeability. The findings demonstrate the potential of pomegranate agro-waste for sustainable product development, showing greater industrial viability while contributing to waste valorization.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"163 ","pages":"Pages 87-104"},"PeriodicalIF":4.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146191855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stimulation of toluene biofiltration under continuous and intermittent operation by a static magnetic field 静电磁场对连续和间歇操作下甲苯生物过滤的刺激作用

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-04-01 Epub Date: 2026-02-11 DOI: 10.1016/j.procbio.2026.02.012

Estheisy López-Bello , Aitor Aizpuru , Sonia Arriaga

{"title":"Stimulation of toluene biofiltration under continuous and intermittent operation by a static magnetic field","authors":"Estheisy López-Bello , Aitor Aizpuru , Sonia Arriaga","doi":"10.1016/j.procbio.2026.02.012","DOIUrl":"10.1016/j.procbio.2026.02.012","url":null,"abstract":"<div><div>The impact of static magnetic field exposure (SMF) was assessed during the biological treatment of toxic toluene vapors. Two identical biofilters were operated, one in the presence of a 50 mT SMF, the other without. The treatment was first carried out with a continuous toluene inlet load of 60 g m<sup>−3</sup>h<sup>−1</sup>, then with discontinuous airflow. During steady-state operation under continuous toluene load, the presence of SMF improved the toluene removal by 23 %, achieving a significantly higher elimination capacity (EC). During the intermittent load, higher robustness regarding shutdowns was noted in the biofilter exposed, with a prompter recovery of removal efficiency (RE) after shutdowns (1.5 h vs.≈2.5 h). Although impacted by the discontinuous load operation, the EC remained significantly higher in the presence of a SMF (42.2 vs. 36.1 g m<sup>−3</sup>h<sup>−1</sup>). The SMF boosted biofilm formation during the startup, generating a 30 % higher biomass. The better toluene removal under the SMF could be linked with higher microbial activity, confirmed by higher CO<sub>2</sub> production. The observed consumption of extracellular polymeric substances (EPS) as an alternative carbon source during discontinuous toluene loads was reduced in the presence of a SMF. These results demonstrated that applying SMFs can be an environmentally friendly technology to enhance the performance of biofiltration.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"163 ","pages":"Pages 127-138"},"PeriodicalIF":4.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146192412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of biomass solid loading on ethanol fermentation with an engineered yeast 生物质固体负荷对工程酵母乙醇发酵的影响

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-04-01 Epub Date: 2026-02-02 DOI: 10.1016/j.procbio.2026.02.002

Ying Tan , Haixin Peng , Mairui Zhang, Linjing Jia, Jikai Zhao

引用次数: 0

Optimizing trickle-bed reactor performance for hydrogenotrophic methane production in power-to-methane systems 电力制甲烷系统中氢营养化甲烷生产的滴流床反应器性能优化

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-04-01 Epub Date: 2026-02-10 DOI: 10.1016/j.procbio.2026.02.010

Claudio L. Kalbermatten , Sara Jordan , Joël F. Pothier , Hans-Joachim Nägele , Theo H.M. Smits , Wolfgang Merkle

{"title":"Optimizing trickle-bed reactor performance for hydrogenotrophic methane production in power-to-methane systems","authors":"Claudio L. Kalbermatten , Sara Jordan , Joël F. Pothier , Hans-Joachim Nägele , Theo H.M. Smits , Wolfgang Merkle","doi":"10.1016/j.procbio.2026.02.010","DOIUrl":"10.1016/j.procbio.2026.02.010","url":null,"abstract":"<div><div>Biomethanation is a process enabling both power-to-methane and biogas upgrading by using methanogenic archaea to convert hydrogen and carbon dioxide to methane. This study describes the long-term operation of a lab-scale mixed culture ex-situ biomethanation trickle bed reactor (TBR) under varying operating conditions such as medium feeding rate, gas loading rate, temperature, pressure, and pH. Additionally, nutrient medium variations with a focus on trace elements were examined. Community analysis of the TBR startup revealed that the fraction of archaeal taxa increased from 0.24 % to 5.1 % and a stable archaeal consortium was established within 21 days after inoculation. Despite this, switching to thermophilic conditions after 239 days of mesophilic operation did not impair reactor performance. Nutrient starvation caused rapid drops in productivity which could be alleviated by higher medium dosing without long adaptation periods. Elevated sodium/potassium ratio and additional trace elements (zinc, boron, calcium, copper, manganese) significantly increased the methane production in batch culture, but this could not be replicated in the TBR. The final TBR methane evolution rate after 388 days of operation was 6.06 ± 0.04 m<sup>3</sup>/m<sup>3</sup>/d at 96.1 % ± 2.8 % CH<sub>4</sub>. These results represent an addition to the growing body of operational data for TBR biomethanation.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"163 ","pages":"Pages 66-76"},"PeriodicalIF":4.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146172126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytodetoxification and microbial community dynamics during the bioremediation of sulfonylurea herbicide contaminated soils by esterase SulE in cross-linked gelatin/chitosan 明胶/壳聚糖交联酯酶胶囊修复磺胺类除草剂污染土壤的植物解毒作用及微生物群落动态

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-04-01 Epub Date: 2026-02-11 DOI: 10.1016/j.procbio.2026.02.011

Xiang Li , Xuanhe Fu , Xu Li , Zhencheng Su , Huiwen Zhang , Zhixiong Yu

{"title":"Phytodetoxification and microbial community dynamics during the bioremediation of sulfonylurea herbicide contaminated soils by esterase SulE in cross-linked gelatin/chitosan","authors":"Xiang Li , Xuanhe Fu , Xu Li , Zhencheng Su , Huiwen Zhang , Zhixiong Yu","doi":"10.1016/j.procbio.2026.02.011","DOIUrl":"10.1016/j.procbio.2026.02.011","url":null,"abstract":"<div><div>Sulfonylurea herbicides exhibit persistence in alkaline soils, which consequently poses risks to both crops and soil microbial communities. This study evaluated the efficacy of gelatin/chitosan-immobilized SulE (GLT/CTS-SulE) for remediating soil contaminated tribenuron-methyl or metsulfuron-methyl, focusing on its phytoremediation effectiveness and impact on soil microbial dynamics. The results demonstrate that GLT/CTS-SulE effectively improved multiple growth indicators of maize, including plant height, main root length, fresh weight and root weight. GLT/CTS-SulE can also effectively maintain the balance of soil microorganisms. It has a mitigating effect on the inhibition of total phospholipid fatty acid (PLFA) concentration, bacterial PLFA concentration and Shannon-Wiener index, as well as the promotion of fungal PLFA concentration by sulfonylurea herbicides. Furthermore, GLT/CTS-SulE demonstrates enhanced remediation capabilities for soil contaminated with tribenuron-methyl or metsulfuron-methyl in comparison to free SulE. In summary, GLT/CTS-SulE presents a stable and efficient strategy for enhancing remediation of soils contaminated with sulfonylurea herbicides <em>in situ</em>.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"163 ","pages":"Pages 105-113"},"PeriodicalIF":4.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146192413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous yeast oil production and in-situ recovery from Rhodotorula toruloides using oil-capturing agents for eco-friendly biolubricant synthesis 利用捕油剂同时从红酵母中提取酵母油并原位回收用于生态友好型生物润滑剂合成

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-04-01 Epub Date: 2026-02-10 DOI: 10.1016/j.procbio.2026.02.014

Kessuda Ngernsombat , Sasitorn Khuntong , Parichat Koedprasong , Thidarat Samranrit , Utt Eiamprasert , Witsanu Srila , Sujaya Ritthisorn , Siritorn Teeravet , Chatrudee Suwannachart , Jantima Teeka , Chiu-Hsia Chiu , Atsadawut Areesirisuk

{"title":"Simultaneous yeast oil production and in-situ recovery from Rhodotorula toruloides using oil-capturing agents for eco-friendly biolubricant synthesis","authors":"Kessuda Ngernsombat , Sasitorn Khuntong , Parichat Koedprasong , Thidarat Samranrit , Utt Eiamprasert , Witsanu Srila , Sujaya Ritthisorn , Siritorn Teeravet , Chatrudee Suwannachart , Jantima Teeka , Chiu-Hsia Chiu , Atsadawut Areesirisuk","doi":"10.1016/j.procbio.2026.02.014","DOIUrl":"10.1016/j.procbio.2026.02.014","url":null,"abstract":"<div><div>Yeast oil is gaining recognition as a sustainable feedstock for biolubricant synthesis. This study focused on enhancing the efficiency of yeast oil production and downstream processing through the simultaneous production of glycerol-based yeast oil by <em>Rhodotorula toruloides</em> TISTR 5186, coupled with an <em>in-situ</em> oil recovery process using oil-capturing agents (OCAs). The Sepabeads™ SP70 OCA was more suitable for simultaneous production and recovery of yeast oil than Amberlite™ XAD-16. Scaling up the fed-batch process with online oil recovery improved biomass yield, total oil yield, and oil content by 2.83, 1.95, and 1.36 folds, respectively, compared to batch operations. The extracted yeast oil was effectively converted to trimethylolpropane ester with an 85.9 % yield, establishing its potential as a viable biolubricant precursor. This approach demonstrated the potential for simultaneous yeast oil production and recovery without cell disruption, offering a sustainable and cost-effective bioprocess for eco-friendly biolubricant synthesis.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"163 ","pages":"Pages 139-149"},"PeriodicalIF":4.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146172127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improved sustainable production of fusel esters from various oleaginous raw materials: Exploring the efficient catalytic performance and reusability of immobilized lipase Eversa® Transform 2.0 从各种含油原料中提高燃料酯的可持续生产：探索固定化脂肪酶Eversa®Transform 2.0的高效催化性能和可重复使用性

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-04-01 Epub Date: 2026-02-02 DOI: 10.1016/j.procbio.2026.01.019

Felipe R. Carlos , Lucas B. Matheus , Raynara P. da Silva , Johan M. Rios Marin , Milson S. Barbosa , Cleide M.F. Soares , Jaine H.H. Luiz , Adriano A. Mendes

{"title":"Improved sustainable production of fusel esters from various oleaginous raw materials: Exploring the efficient catalytic performance and reusability of immobilized lipase Eversa® Transform 2.0","authors":"Felipe R. Carlos , Lucas B. Matheus , Raynara P. da Silva , Johan M. Rios Marin , Milson S. Barbosa , Cleide M.F. Soares , Jaine H.H. Luiz , Adriano A. Mendes","doi":"10.1016/j.procbio.2026.01.019","DOIUrl":"10.1016/j.procbio.2026.01.019","url":null,"abstract":"<div><div>This study aims to improve the production of fusel esters from various oleaginous raw materials using lipase Eversa® Transform 2.0 (ET2.0) immobilized on poly(styrene-divinylbenzene) beads (PSty-DVB-ET2.0) via interfacial activation. The influence of key operational parameters on the esterification of free fatty acids (FFAs) from used soybean cooking oil (USCO) and fusel oil (FO) in solvent-free systems was evaluated using a statistical approach. Under optimal conditions (40 ºC, FFAs:FO molar ratio of 1:2.5, and biocatalyst concentration of 21 wt%), the FFAs conversion reached nearly 90 % within 60 min. By contrast, using free ET2.0 under the same conditions resulted in a maximum FFAs conversion of only 25 %. Similar trends were observed for FFAs derived from canola, corn, soybean, and macauba pulp oils, while free lipase showed the highest productivity in esterification reactions with FFAs from castor oil. PSty-DVB-ET2.0 exhibited excellent reusability, retaining nearly all of its activity after twenty esterification batches. The fusel esters produced from USCO demonstrated to be biodegradable, highly thermally stable up to 200 ºC, and exhibited good fluidity even at low temperatures. This study highlights the promising potential of PSty-DVB-ET2.0 for the sustainable production of fusel esters from a range of oleaginous raw materials, including waste oil (USCO).</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"163 ","pages":"Pages 1-15"},"PeriodicalIF":4.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146102597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anaerobic treatment of real cosmetic wastewater in a lab-scale UASB using fungal biocatalysts: COD/TOC removal and methane yield (Pleurotus ostreatus vs Trametes versicolor) 在实验室规模的UASB中使用真菌生物催化剂厌氧处理真实化妆品废水：COD/TOC去除率和甲烷产率（平菇vs彩板菌）

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-04-01 Epub Date: 2026-02-09 DOI: 10.1016/j.procbio.2026.02.007

Barbaros Durmuş, Nilüfer Nacar Koçer, Gülşad Uslu şenel

{"title":"Anaerobic treatment of real cosmetic wastewater in a lab-scale UASB using fungal biocatalysts: COD/TOC removal and methane yield (Pleurotus ostreatus vs Trametes versicolor)","authors":"Barbaros Durmuş, Nilüfer Nacar Koçer, Gülşad Uslu şenel","doi":"10.1016/j.procbio.2026.02.007","DOIUrl":"10.1016/j.procbio.2026.02.007","url":null,"abstract":"<div><div>Real cosmetic wastewater is chemically complex and often difficult to treat using conventional processes. This study investigated continuous anaerobic treatment in a 3.0-L upflow anaerobic sludge blanket (UASB) reactor operated with polyurethane-immobilized white-rot fungi, Pleurotus ostreatus and <em>Trametes versicolor</em>. Real plant effluent was fed under hydraulic retention times (HRT) of 15, 30, and 45 h to evaluate pollutant removal and biogas performance. The reactor remained stable throughout operation, with effluent pH between 6.4 and 6.7 and intact fungal pellets. COD removal increased with HRT, reaching 84.0 % for <em>P. ostreatus</em> and 90.4 % for <em>T. versicolor</em> at 45 h. TOC removal followed a similar trend, attaining 78.0 % and 76.0 %, respectively. Color removal reached 68.7 % for <em>P. ostreatus</em> and 79.5 % for <em>T. versicolor</em>. Cumulative biogas production was 300 mL for <em>P. ostreatus</em> and 250 mL for <em>T. versicolor</em>, with methane fractions of 60 % and 55 %. Specific methane yields increased with HRT, achieving 178 and 165 mL CH₄ g⁻¹ VS at 45 h. Overall<em>, T. versicolor</em> exhibited superior decolorization, whereas <em>P. ostreatus</em> produced more methane-rich biogas. These findings demonstrate the potential of fungal UASB systems as low-energy, biologically driven treatment options for dye-rich cosmetic wastewater.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"163 ","pages":"Pages 58-65"},"PeriodicalIF":4.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146191853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0