Process Biochemistry最新文献

Carbonate-enhanced cultivation of microalgae biofilm on the bovine serum albumin coated hollow fiber membranes 微藻生物膜在牛血清白蛋白包被中空纤维膜上的碳酸盐强化培养

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-17 DOI: 10.1016/j.procbio.2025.06.010

Jia Xin Yap , C.P. Leo , D.J.C. Chan , Nazlina Haiza Mohd Yasin

{"title":"Carbonate-enhanced cultivation of microalgae biofilm on the bovine serum albumin coated hollow fiber membranes","authors":"Jia Xin Yap , C.P. Leo , D.J.C. Chan , Nazlina Haiza Mohd Yasin","doi":"10.1016/j.procbio.2025.06.010","DOIUrl":"10.1016/j.procbio.2025.06.010","url":null,"abstract":"<div><div>Microalgae has been commercially grown to support the growth of biorefineries. Since microalgae are commonly grown using carbon dioxide from the air during biofilm cultivation, their efficiency in capturing carbon from a liquid medium remains unclear. In this study, sodium bicarbonate (NaHCO<sub>3</sub>) with varied concentrations was added as the additional carbon source during the cultivation of <em>Navicula incerta</em> on the bovine serum albumin (BSA)-coated hollow fiber membranes. <em>N. incerta</em>, a biofilm-forming diatom known for its surface adherence and environmental robustness, was selected for its suitability in the hollow fiber membrane cultivation system. The highest growth rate of 81.7 % was obtained by adding 8 g/L of NaHCO<sub>3</sub>, with a harvesting efficiency of 81.02 %. The carbon fixation rate of 375.45 ± 15.89 mg˖L<sup>−1</sup>˖d<sup>−1</sup> and carbon consumption efficiency of 58.91 ± 6.96 % were further attained. The chlorophyll content of <em>N. incerta</em> also increased from 2.61 ± 0.22–4.22 ± 0.28 mg/L. The microalgae biofilm on the BSA-coated PVDF hollow fiber membranes not only absorbed carbon dioxide from the air, but also removed the carbon source from the liquid.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 300-307"},"PeriodicalIF":3.7,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144322564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel spider-toxin-derived anti-gout peptide NCTX14 alleviated NLRP3-mediated pyroptosis in hyperuricemia 一种新型蜘蛛毒素衍生的抗痛风肽NCTX14减轻了nlrp3介导的高尿酸血症热亡

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-16 DOI: 10.1016/j.procbio.2025.06.009

Naixin Liu , Yixiang Liu , Junyuan Wang , Yuliu Yang , Yujing Ding , Xiaohan Sun , Mengfan Ma , Xin Yu , Shaoyang Zhang , Jing Tang , Xinwang Yang , Ying Wang

{"title":"A novel spider-toxin-derived anti-gout peptide NCTX14 alleviated NLRP3-mediated pyroptosis in hyperuricemia","authors":"Naixin Liu , Yixiang Liu , Junyuan Wang , Yuliu Yang , Yujing Ding , Xiaohan Sun , Mengfan Ma , Xin Yu , Shaoyang Zhang , Jing Tang , Xinwang Yang , Ying Wang","doi":"10.1016/j.procbio.2025.06.009","DOIUrl":"10.1016/j.procbio.2025.06.009","url":null,"abstract":"<div><div>Gout is a serious metabolic disorder mainly caused by high levels of serum uric acid (HUA). Due to the limitations of current treatments, it is important to investigate new urate-lowering compounds. In this study, a novel peptide, NCTX14 (CRPPVMLPKCEEFMK), was discovered within the venom gland of <em>Nephila clavata</em>. Research shows that NCTX14 doesn't exhibit cytotoxicity or hemolytic activity <em>in vitro</em> and is non-toxic when administered <em>in vivo</em>. This peptide effectively reduces serum uric acid levels and inhibits xanthine oxidase activity in mice with high uric acid levels. This is achieved through the downregulation of URAT1 and GLUT9, along with the upregulation of OAT1, ultimately enhancing uric acid excretion. Moreover, NCTX14 alleviates kidney damage by inhibiting the expression of inflammatory cytokines, such as IL-6, IL-1β, TNF-α. In HUA nephropathy, NCTX14 alleviates renal injury by inhibiting NLRP3 mediated pyroptosis injury. Additionally, NCTX14 demonstrates anti-inflammatory and pain-relieving effects in mice. The potential of NCTX14 in the treatment of gout suggests its practical application value as an anti-gout medicine candidate molecular.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 10-20"},"PeriodicalIF":3.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144297810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fast and green process to obtain astaxanthin-rich extracts from shrimp shell waste for dye sensitized solar cells 从虾壳废料中提取富含虾青素的染料敏化太阳能电池的快速绿色工艺

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-16 DOI: 10.1016/j.procbio.2025.06.006

Francesco Milano , Federica Mancarella , Livia Giotta , Ilaria Citro , Stefano Trocino , Giuseppe Calogero , Donatella Spadaro

{"title":"Fast and green process to obtain astaxanthin-rich extracts from shrimp shell waste for dye sensitized solar cells","authors":"Francesco Milano , Federica Mancarella , Livia Giotta , Ilaria Citro , Stefano Trocino , Giuseppe Calogero , Donatella Spadaro","doi":"10.1016/j.procbio.2025.06.006","DOIUrl":"10.1016/j.procbio.2025.06.006","url":null,"abstract":"<div><div>Aiming to address the critical need for renewable energy, this study explores the potential of astaxanthin-rich extracts from shrimp waste as natural sensitizers for dye-sensitized solar cells (DSSCs) using an eco-friendly and economically viable process. Photoconversion efficiencies (PCE) of astaxanthin-based DSSCs were evaluated through I-V measurements while charge transfer resistances (R<sub>CT</sub>) were assessed by electrochemical impedance spectroscopy. The sensitizer preparation involved extraction of astaxanthin from waste shrimp (<em>Aristaeomorpha foliacea</em>) cephalothoraxes, which were desiccated, finely milled and treated with ethyl acetate, to obtain a crude extract very rich in astaxanthin mainly present in esterified forms. The extraction process was fast and avoided extreme experimental conditions in terms of energy consumption, ultra-vacuum, high temperatures and use of toxic solvents. Direct application of the crude extract as sensitizer for DSSCs resulted in low PCE (0.09 %) and high R<sub>CT</sub>, due to the limited ability of esterified astaxanthin to interact with the TiO₂ layer. This limitation was overcome through saponification of the extract with methanolic NaOH, leading to free astaxanthin with high recovery yield and limited degradation. The saponified extract showed lower R<sub>CT</sub> and higher PCE (0.30 %). These results highlight the promising potential of suitably processed shrimp waste as a source of sensitizers for DSSCs.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 308-316"},"PeriodicalIF":3.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144322565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and optimization of a biological coating process based on chitosan from edible cricket to control Aspergillus niger-decay and weight loss of the Algerian dates cv. Deglet Nour 食用蟋蟀壳聚糖生物包衣工艺的开发与优化，以防治阿尔及利亚枣黑曲霉的腐烂和失重。Deglet努尔

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-16 DOI: 10.1016/j.procbio.2025.06.007

Imane Brahimi , Khadidja Allali , Abderrahmane Benadjila , Miyada Zamoum , Yacine Goudjal

{"title":"Development and optimization of a biological coating process based on chitosan from edible cricket to control Aspergillus niger-decay and weight loss of the Algerian dates cv. Deglet Nour","authors":"Imane Brahimi , Khadidja Allali , Abderrahmane Benadjila , Miyada Zamoum , Yacine Goudjal","doi":"10.1016/j.procbio.2025.06.007","DOIUrl":"10.1016/j.procbio.2025.06.007","url":null,"abstract":"<div><div>This study aims at reducing the post-harvest <em>Aspergillus niger</em> decay incidence of date palm through the biological coating process. To determine the most effective process, chitosan extracted from edible cricket (<em>Schistocerca gregaria</em>) combined with Arabic gum, Aloe vera gel or carboxymethyl cellulose was screened in comparison with the chemical fungicide Benomyl®. Similarly to the Benomyl®, coating dates with chitosan combined with Arabic gum significantly reduced the decay incidence (10 %) compared to untreated dates (100 %) and the weight losses were decreased from 6.02 % to 3.36 %. Coating dates with chitosan added to Arabic gum was selected for optimization using the Plackett-Burman and response surface methodologies approach. Acetic acid, gum Arabic and chitosan quantities were found to be the significant factors influencing the efficiency of the coating bioprocess. The optimized coating bioprocess was experimentally confirmed for the stored dates at 4 °C for 30 days and showed a complete elimination of <em>A</em>. <em>niger</em> decay incidence. The enhanced biological coating process based on chitosan added to gum can be proposed as a promising sustainable solution to reduce the abusive uses of chemical fungicides in <em>Deglet Nour</em> dates and to cut down weight losses during storage.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 21-32"},"PeriodicalIF":3.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144322761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The optimized signal peptide and feeding strategy to enhance secretory expression of LegH in Komagataella phaffii 优化信号肽及摄食策略对提高法菲小鹿LegH分泌表达的影响

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-09 DOI: 10.1016/j.procbio.2025.06.005

Hongtao Chen , Yueheng Niu , Liuxia Zhang , Yunpeng Wang , Ao Luo , Zhihui Dai , Guocheng Du , Xinrui Zhao

引用次数: 0

Nutraceuticals enhanced by nanotechnology: A new frontier for obesity treatment 纳米技术增强的营养保健品：肥胖症治疗的新前沿

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-06 DOI: 10.1016/j.procbio.2025.05.021

Sri Renukadevi Balusamy , Selvakumar Vijayalakshmi , Sumathi Sundaravadivelu , Md Amdadul Huq , Sagnik Nag , Sourav Mohanto , Johan Sukweenadhi , Deong Hwan Oh , Haribalan Perumalsamy

{"title":"Nutraceuticals enhanced by nanotechnology: A new frontier for obesity treatment","authors":"Sri Renukadevi Balusamy , Selvakumar Vijayalakshmi , Sumathi Sundaravadivelu , Md Amdadul Huq , Sagnik Nag , Sourav Mohanto , Johan Sukweenadhi , Deong Hwan Oh , Haribalan Perumalsamy","doi":"10.1016/j.procbio.2025.05.021","DOIUrl":"10.1016/j.procbio.2025.05.021","url":null,"abstract":"<div><div>Nutraceuticals encompass bioactive compounds like polyphenols, flavonoids, and essential fatty acids inherent in food that play defensive roles against human ailments. Presently, the pharmaceutical industry is focused on nutraceutical-based nanomaterials to mitigate drawbacks in obesity treatment. The use of nanomaterials enhances bioavailability, stability, and targeted drug delivery, diminishing side effects. This review delineates the significance, limitations, and molecular mechanisms of nutraceuticals' pharmacological properties, emphasizing how nutraceutical-based nanomaterials address challenges for effective obesity treatment. We outline potential nano-based anti-obesity drugs for heightened pharmacological impact and acknowledge associated clinical challenges and limitations. Critically, we evaluate the advantages and disadvantages of nutraceuticals, advocating various delivery systems to enhance their anti-obesity efficacy. Notably, nutraceutical-based nanoparticles offer increased bioavailability, precise distribution, reduced side effects, and prolonged sustainability, revolutionizing obesity management. Conscious implementation of nutraceutical-based nanoparticles holds promise in alleviating complications associated with conventional nutraceuticals in obesity management.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 217-235"},"PeriodicalIF":3.7,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144240181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of ultrasound-assisted pH shift treatment on structure and functional properties of mulberry leaf protein 超声辅助pH转移处理对桑叶蛋白结构和功能特性的影响

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-06 DOI: 10.1016/j.procbio.2025.06.004

Ke Li , Qiongying Wu , Junqiang Jia

{"title":"Effect of ultrasound-assisted pH shift treatment on structure and functional properties of mulberry leaf protein","authors":"Ke Li , Qiongying Wu , Junqiang Jia","doi":"10.1016/j.procbio.2025.06.004","DOIUrl":"10.1016/j.procbio.2025.06.004","url":null,"abstract":"<div><div>This study investigated the impact of multifrequency ultrasound (22/28/40 kHz)-assisted pH shift treatment on the structural and functional attributes of mulberry leaf protein (MLP). The results showed that ultrasound-assisted pH shift increased the solubility of MLP by 281.74 % and significantly increased its emulsifying activity index (from 188.63 ± 3.27–231.41 ± 5.22 m²/g), emulsion stability index (from 51.22 ± 2.67–136.20 ± 9.98) and in vitro protein digestibility (from 41.75 % ± 3.57–72.54 % ± 1.38 %). The reduction in MLP particle size and the increase in zeta potential indicated that the treatment disrupted the tertiary structure. Furthermore, the increases in ultraviolet absorbance, fluorescence intensity, and molecular flexibility revealed significant alterations in the secondary and tertiary structures induced by ultrasound-assisted pH shift. The treatment reduced the α-helix content, while increased the β-sheet content, suggesting partial unfolding in the secondary structure of MLP, which led to the increase of exposed hydrophobic groups. As a result, the surface hydrophobicity of MLP was increased after ultrasound-assisted pH shift treatment. In conclusion, ultrasound-assisted pH shift treatment holds promise for advancing the application of MLP in the food industry.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 244-254"},"PeriodicalIF":3.7,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144262486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Purification of messenger RNA directly from crude IVT using polyethylene glycol and NaCl precipitation 用聚乙二醇和NaCl沉淀法直接从粗IVT中纯化信使RNA

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-06 DOI: 10.1016/j.procbio.2025.06.002

Maria del Carme Pons Royo , Tyler Arnold , Isabella Perez Rodriguez , Nicole Ostrovsky , Mushriq Al-Jazrawe , Andrew Hatas , Vico Tenberg , Allan S. Myerson , Richard D. Braatz

{"title":"Purification of messenger RNA directly from crude IVT using polyethylene glycol and NaCl precipitation","authors":"Maria del Carme Pons Royo , Tyler Arnold , Isabella Perez Rodriguez , Nicole Ostrovsky , Mushriq Al-Jazrawe , Andrew Hatas , Vico Tenberg , Allan S. Myerson , Richard D. Braatz","doi":"10.1016/j.procbio.2025.06.002","DOIUrl":"10.1016/j.procbio.2025.06.002","url":null,"abstract":"<div><div>The increasing demand for mRNA-based therapeutics requires scalable and cost-effective purification methods. Chromatography-based approaches, such as Oligo-dT affinity chromatography, require multiple processing steps, including buffer exchange and high-temperature treatments, which can lead to mRNA degradation and increased costs. Although precipitation is commonly used at the lab scale, its application in large-scale mRNA purification remains underexplored. In this study, we developed a rapid and efficient precipitation-based method for mRNA purification. The effects of different precipitation conditions, including salt type, co-precipitating agents, and time, were evaluated in terms of recovery yield and purity. Precipitation experiments were conducted for two different in vitro transcription crude materials containing mRNAs of varying lengths and concentrations. The optimal conditions, identified as a combination of NaCl and PEG 6000, achieved recovery yields of 80–93 % and purities of 80–83 %, with no detectable double-stranded RNA formation or fragmentation. The precipitated mRNAs were successfully transfected into 293 T and A549 cells, showing protein expression comparable to commercial and chromatography-purified mRNAs. These findings position PEG/NaCl precipitation as a rapid, scalable, and cost-effective alternative to traditional chromatography methods, significantly reducing process complexity and time for large-scale mRNA production.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 263-273"},"PeriodicalIF":3.7,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144262487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Static magnetic field enhances the purification of gaseous n-hexane in airlift two-phase partitioning bioreactor 静磁场增强了气升式两相分配生物反应器中气态正己烷的净化效果

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-06 DOI: 10.1016/j.procbio.2025.06.003

Qian Li , Xianhao Lu , Keping Zhang , Xianwang Kong , Lichao Lu , Jianmeng Chen , Dongzhi Chen

{"title":"Static magnetic field enhances the purification of gaseous n-hexane in airlift two-phase partitioning bioreactor","authors":"Qian Li , Xianhao Lu , Keping Zhang , Xianwang Kong , Lichao Lu , Jianmeng Chen , Dongzhi Chen","doi":"10.1016/j.procbio.2025.06.003","DOIUrl":"10.1016/j.procbio.2025.06.003","url":null,"abstract":"<div><div>Two-phase partitioning bioreactor (TPPB) has been developed as a promising approach to improve the biological purification of hydrophobic waste-gas. However, the biodegradation efficiency of highly hydrophobic pollutants remains limited. Herein, static magnetic field (SMF) was innovatively introduced to enhance the biodegradation of the model pollutant <em>n</em>-hexane and the underlying mechanism was revealed. Results showed that the influence of SMF on different <em>n</em>-hexane degrading bacteria varied significantly, and SMF cast the most pronounced enhancement on <em>Pseudomonas</em> HY-4 under the optimized intensity of 20 mT. The removal efficiency of <em>n</em>-hexane with an inlet concentration of 540·mg m<sup>−3</sup> using the SMF enhanced TPPB reactor reached 93 % within 5 days, surpassing the unenhanced bioreactor (82 %). And the SMF enhanced reactor retained the original shock load resistance. Mechanism analysis revealed that, under the action of SMF, the microbial cell surface hydrophobicity was sustainably improved, thereby improving the mass transfer of <em>n</em>-hexane diffusing from the liquid to the microbial phase. The electron transport chain activity in the SMF-enhanced TPPB was enhanced by 10.3 %. Furthermore, the abundance of degrading bacteria beneficial to <em>n</em>-hexane degradation was greatly enriched. This work may lay a foundation for the application of SMF in enhancing the biodegradation of hydrophobic gaseous pollutants.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 209-216"},"PeriodicalIF":3.7,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144241090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combinatorial engineering of microbial cell factory for 2′-fucosyllactose biosynthesis 2′-焦酰基乳糖生物合成微生物细胞工厂的组合工程

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-04 DOI: 10.1016/j.procbio.2025.05.024

Jingyu Zhou , Xinyue Su , Yongzhong Wang , Qiang Ding

{"title":"Combinatorial engineering of microbial cell factory for 2′-fucosyllactose biosynthesis","authors":"Jingyu Zhou , Xinyue Su , Yongzhong Wang , Qiang Ding","doi":"10.1016/j.procbio.2025.05.024","DOIUrl":"10.1016/j.procbio.2025.05.024","url":null,"abstract":"<div><div>2’-fucosyllactose (2’-FL), a predominant human milk oligosaccharide (HMOs), has showed significant potential in infant health applications including intestinal protection, cognitive development, and immune system modulation. Therefore, to enhance microbial biosynthesis of 2’-FL, we engineered a de novo synthesis pathway through deleting key enzymes gene: <em>wecB</em> (encoding UDP-N-acetyl glucosamine-2-epimerase), <em>lacZ</em> (encoding β-galactosidase), <em>wcaJ</em> (encoding UDP-glucose lipid carrier transferase), which could increase the 2’-FL titer and productivity to 2.25 g/L and 0.03 g/L/h, respectively. Furthermore, optimization of induction time further improved production performance to 4.06 g/L and 0.06 g/L/h. Through systematic gene deletion analysis, we identified <em>lacA</em> and <em>iclR</em> as critical metabolic nodes. Their knockout negatively impacted 2’-FL biosynthesis by reducing lactose availability and disturbing metabolic flux. Finally, the 2’-FL was furthermore improved to 4.75 g/L through expressing <em>gsk</em> and <em>zwf</em> genes for NADPH and GTP regeneration. This study can effectively increase the production of 2’-fucosyllactose, which can provide a research basis for the large-scale bioproduction of 2’-fucosyllactose in the industrial factory.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 202-208"},"PeriodicalIF":3.7,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144230402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0