Process Biochemistry最新文献_第2页

Pea protein isolate and sour cherry polyphenols interaction improved the stability of polyphenols and the efficacy in alleviating colitis 豌豆分离蛋白与酸樱桃多酚相互作用提高了多酚的稳定性和缓解结肠炎的疗效

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-07-12 DOI: 10.1016/j.procbio.2025.07.008

Guihua Wang, Bingjie Liu, Xianghong Meng

{"title":"Pea protein isolate and sour cherry polyphenols interaction improved the stability of polyphenols and the efficacy in alleviating colitis","authors":"Guihua Wang, Bingjie Liu, Xianghong Meng","doi":"10.1016/j.procbio.2025.07.008","DOIUrl":"10.1016/j.procbio.2025.07.008","url":null,"abstract":"<div><div>To enhance the bioavailability of sour cherry polyphenol extract (SCE), pea protein isolate (PPI) with low allergenicity was selected to form the complex with SCE. The storage and digestion characteristics of the complex, along with its effects on relieving colitis were evaluated. The results showed that after complexing with PPI, the storage stability of SCE was improved. After digestion, the PSA group (PPI-SCE complex prepared at pH 3) delivered more polyphenols to the small intestine. PSA was more effective in alleviating ulcerative colitis compared with SCE alone, which inhibited the excessive activation of the TLR4/NF-κB pathway. The shortening of the colon length was mitigated. It was manifested in a reduction in inflammatory factor levels, alleviation of colonic damage, and less tissue structure destruction. Meanwhile, PSA upregulated the gene expression of tight-junction proteins such as zonula occludens-1, occludin, and claudin-4, enhancing the integrity of the intestinal barrier and thus alleviating the symptoms of ulcerative colitis. This study provided a reference for the high-value utilization of SCE and the extensive application of PPI.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 112-121"},"PeriodicalIF":3.7,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144654916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasound-enhanced delivery of pterostilbene-loaded long-circulating solid lipid nanoparticles for the inhibition of IL-6/STAT3 signaling pathway in ovarian cancer 超声增强的紫芪载长循环固体脂质纳米颗粒对卵巢癌中IL-6/STAT3信号通路的抑制作用

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-07-09 DOI: 10.1016/j.procbio.2025.06.017

Wenjing Wang, Haibin Xi, Yi Ping

{"title":"Ultrasound-enhanced delivery of pterostilbene-loaded long-circulating solid lipid nanoparticles for the inhibition of IL-6/STAT3 signaling pathway in ovarian cancer","authors":"Wenjing Wang, Haibin Xi, Yi Ping","doi":"10.1016/j.procbio.2025.06.017","DOIUrl":"10.1016/j.procbio.2025.06.017","url":null,"abstract":"<div><div>The current investigation explores a novel combinational approach for ovarian cancer treatment by formulating Pterostilbene-loaded solid lipid nanoparticles (PT-SLNPs) and enhancing their therapeutic efficiency using ultrasound stimulation. The formulated PT-SLNPs displays favorable physicochemical properties, with a mean particle size of 133.46 ± 24.21 nm, a low polydispersity index (0.14 ± 0.02), and high entrapment efficiency (87.76 ± 0.5 %). The uniqueness of this work is attributed to the synergistic application of PT-SLNPs and ultrasound (US), which significantly enhanced the cytotoxic effect against human ovarian cancer cell A2780. Further, flowcytometry method confirmed a considerable increase in apoptosis in cells treated with PT-SLNPs + US combination. Mechanistically, exposure of cancer cells to PT-SLNPs + US downregulated the key tumor associated pro-survival and proliferative pathways by suppressing IL-6 expression and inhibiting STAT3 phosphorylation. These findings highlight the innovative potential of US assisted PT-SLNPs as a promising, less toxic alternative to conventional chemotherapy and radiotherapy in ovarian cancer treatment.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 122-135"},"PeriodicalIF":3.7,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144654917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiplex metabolic engineering for enhanced indole-3-acetic acid production via optimized biosynthetic pathways in E. coli 通过优化大肠杆菌生物合成途径提高吲哚-3-乙酸生产的多重代谢工程

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-07-09 DOI: 10.1016/j.procbio.2025.07.006

Fabien Nsanzabera , Binbin Liu

{"title":"Multiplex metabolic engineering for enhanced indole-3-acetic acid production via optimized biosynthetic pathways in E. coli","authors":"Fabien Nsanzabera , Binbin Liu","doi":"10.1016/j.procbio.2025.07.006","DOIUrl":"10.1016/j.procbio.2025.07.006","url":null,"abstract":"<div><div>IAA is a key auxin that regulates plant growth and development. Engineering <em>E. coli</em> as a microbial cell factory provides a sustainable alternative to petrochemical-based production methods by using glucose as a feedstock. This review outlines fundamental strategies that enhance microbial IAA synthesis while simplifying technical concepts for broader understanding. Key approaches include overexpressing genes in the shikimate pathway and modifying central carbon metabolism (PP and EMP pathways) to increase precursor availability, particularly E4P and PEP. Enhancing cofactor supply, through improved NAD(P)H and ATP regeneration and FMN and FAD preservation, supports the energy and redox demands of IAA biosynthesis. Enhancing intracellular L-tryptophan levels by boosting import and limiting efflux further contributes to IAA productivity. Balancing growth with metabolite output ensures process efficiency. The review highlights forward-looking strategies, including machine learning for pathway design, genome-scale modeling to identify bottlenecks, and the use of modular or co-culture engineering to optimize microbial IAA biosynthesis. It underscores the potential of engineered <em>E. coli</em> as a sustainable platform for IAA production, paving the way for greener agricultural practices and reduced reliance on petrochemicals.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 147-161"},"PeriodicalIF":3.7,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144654915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic engineering of Yarrowia lipolytica to produce menaquinone-7 溶脂耶氏菌代谢工程生产甲基萘醌-7

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-07-09 DOI: 10.1016/j.procbio.2025.07.007

Yeqiao Shen , Xuezhen Wang , Shoubo Zuo , Zhongmin Tang , Zhengjun Xiong , Jie Xiong , Huili Zhang

{"title":"Metabolic engineering of Yarrowia lipolytica to produce menaquinone-7","authors":"Yeqiao Shen , Xuezhen Wang , Shoubo Zuo , Zhongmin Tang , Zhengjun Xiong , Jie Xiong , Huili Zhang","doi":"10.1016/j.procbio.2025.07.007","DOIUrl":"10.1016/j.procbio.2025.07.007","url":null,"abstract":"<div><div>Menaquinone-7 (MK-7), a vital subtype of fat-soluble vitamin K2, plays essential physiological roles in preventing osteoporosis, vascular calcification, and reducing vascular damage. Its broad applications in functional foods and pharmaceuticals have attracted significant attention. <em>Yarrowia lipolytica</em>, with its abundant acetyl-CoA supply and functional mevalonate (MVA) pathway for synthesizing the polyprenyl side chain of MK-7, demonstrates exceptional capabilities in transporting and storing hydrophobic compounds, making it an ideal host for MK-7 production. In this study, we developed an engineered strain of <em>Y. lipolytica</em> to achieve high-titer production of MK-7 through supplementation with exogenous precursors, 1,4-dihydroxy-2-naphthoic acid (DHNA) or menadione (VK3). By employing metabolic engineering, peroxisome engineering, and fermentation optimization, the final strain YQ-9 produced 255 ± 0.58 mg/L MK-7 after 144 h of shake-flask fermentation, representing the highest reported titer in <em>Y. lipolytica</em> to date. This study not only lays the foundation for the future de novo MK-7 synthesis in <em>Y. lipolytica</em> but also provides novel insights for industrial-scale MK-7 production<em>.</em></div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 101-110"},"PeriodicalIF":3.7,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144604886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Promiscuous enzymatic synthesis of (S)-ibuprofen amides and assessment of their geroprotective and gastroprotective activities in Drosophila melanogaster (S)-布洛芬酰胺在黑腹果蝇中的混酶合成及其对衰老和胃保护作用的评价

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-07-04 DOI: 10.1016/j.procbio.2025.07.004

Nour elhouda Guenifi , Samira Kilani-Morakchi , Mounia Merabet-Khelassi , Maroua Ferdenache , Karima Sifi , Saoussen Zeror

{"title":"Promiscuous enzymatic synthesis of (S)-ibuprofen amides and assessment of their geroprotective and gastroprotective activities in Drosophila melanogaster","authors":"Nour elhouda Guenifi , Samira Kilani-Morakchi , Mounia Merabet-Khelassi , Maroua Ferdenache , Karima Sifi , Saoussen Zeror","doi":"10.1016/j.procbio.2025.07.004","DOIUrl":"10.1016/j.procbio.2025.07.004","url":null,"abstract":"<div><div>This study focuses on the optimization of the biocatalytic amidation of (<em>S</em>)-Ibuprofen [IBU], with primary amines under promiscuous enzymatic conditions. The use of drying agent as magnesium sulfate and Montmorillonite K10 instead of molecular sieves 4 Å, improved significantly the amidation rates, achieving yields of up to 90 %. The reaction was efficiently promoted by the <em>Novozym</em>®<em>435</em> and enabled the synthesis of three (<em>S</em>)-Ibuprofen amide derivatives: (<em>S</em>)-2-(4-isobutylphenyl)-<em>N</em>-phenyl propanamide <strong>(2)</strong>, (<em>S</em>)-2-(4-isobutylphenyl)-<em>N</em>-(4-(trifluoromethyl) phenyl) propanamide <strong>(3)</strong> and (<em>S</em>)-<em>N</em>-benzyl-2-(4-iso-butylphenyl) propanamide (<strong>4</strong>). In order to select the most suitable prodrug candidate, the synthesized amides were evaluated for predicted toxicity risks, drug-like properties and bioactivity scores using the online tools OSIRIS property explorer and Molinspiration. The compound <strong>(</strong><em><strong>S</strong></em><strong>)-4</strong> [BZA] appeared to be the most promising and was selected to evaluate its geroprotective and gastroprotective activity using <em>Drosophila melanogaster</em> as a powerful model for drug discovery and screening. The <em>in vivo</em> results indicate that this amide prodrug enhances the geroprotective potency by increasing female’s survival, male’s locomotors reactivity and female’s fecundity, as compared to its parent drugs. A significant reduction in the gastric side effects associated with (<em>S</em>)-ibuprofen was also observed after the amidation process.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 93-100"},"PeriodicalIF":3.7,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144589080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic engineering Corynebacterium glutamicum for production of γ-aminobutyric acid by glutamate decarboxylase active at near-neutral pH and displaying at cell surface 代谢工程谷氨酸棒状杆菌利用谷氨酸脱羧酶生产γ-氨基丁酸，该酶在接近中性的pH值下具有活性，并在细胞表面显示

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-07-04 DOI: 10.1016/j.procbio.2025.07.002

Haoqian Li , Hao Yang , Feng Shi

{"title":"Metabolic engineering Corynebacterium glutamicum for production of γ-aminobutyric acid by glutamate decarboxylase active at near-neutral pH and displaying at cell surface","authors":"Haoqian Li , Hao Yang , Feng Shi","doi":"10.1016/j.procbio.2025.07.002","DOIUrl":"10.1016/j.procbio.2025.07.002","url":null,"abstract":"<div><div>γ-Aminobutyric acid (GABA) is widely applied in pharmaceuticals, foods and feeds. <em>Corynebacterium glutamicum</em> that expresses exogenous glutamate decarboxylase (GAD) gene <em>gad</em> can produce GABA from glucose using self-produced L-glutamate. However, the incongruity between optimal pH for cell growth (7.0–7.5) and GAD (4.0–5.0) severely restricts the production of GABA. In this study, several GADs active at near-neutral pH were separately expressed in <em>C. glutamicum</em> by plasmid<em>, Ls</em>GAD derived from <em>Lactobacillus senmaizukei</em> performed better and generated 10.9 g/L GABA. Subsequently, to perform the GAD reaction at the more acidic extracellular environment, <em>Ls</em>GAD was displayed on cell surface by several anchoring motifs, and displaying by PorH and NCgl1307 motifs produced 9.9 g/L and 1.3 g/L GABA, respectively. To further improve GABA production, the metabolic pathways were modified and accompanied by integrating several <em>gad</em> genes in the chromosome, the best strain GSL-6 could produce 15.6 g/L GABA. Finally, the surface display plasmid of <em>Ls</em>GAD was introduced into the chromosomally modified strain GSL-6 to catalyze GAD reaction both intracellularly and extracellularly, and 25.3 g/L and 42.3 g/L GABA was finally produced by shake flask and fed-batch fermentation, respectively. Thereby, this synergistic strategy is beneficial for GABA production in <em>C. glutamicum</em>.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 75-83"},"PeriodicalIF":3.7,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144580830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Properties of Leuconostoc lactis isolated from traditional fermented rice beverages as a starter 从传统发酵米酒中分离的乳酸菌的特性研究

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-07-04 DOI: 10.1016/j.procbio.2025.06.019

Hikaru Ogura, Nanase Okamoto, Ayaka Nakamura, Hajime Takahashi, Riko Kamata, Takashi Kuda

{"title":"Properties of Leuconostoc lactis isolated from traditional fermented rice beverages as a starter","authors":"Hikaru Ogura, Nanase Okamoto, Ayaka Nakamura, Hajime Takahashi, Riko Kamata, Takashi Kuda","doi":"10.1016/j.procbio.2025.06.019","DOIUrl":"10.1016/j.procbio.2025.06.019","url":null,"abstract":"<div><div>The health benefits of traditional fermented rice beverages have attracted considerable attention. “Miki” from the Amami Ohshima island is made from spontaneously fermented rice saccharified with sweet potato <em>β</em>-amylase. <em>Leuconostoc lactis</em> is the dominant species isolated from Miki. In this study, we aimed to clarify the role of indigenous <em>Ln. lactis</em> in fermented rice beverages by determining the antimicrobial activity, exopolysaccharide (EPS) production capacity, and Miki fermentation properties. Among the isolates, <em>Ln. lactis</em> Miki-A3 showed superior anti-listerial activity in the agar overlay method and EPS production with antioxidant capacity (O<sub>2</sub><sup>-</sup> radical scavenging and Fe-reducing power) compared with those of the type strain. The antibiotic resistance and related genes of Miki-A3 were similar to those of the type strain. The positions and orders of EPS-related genes differed between the two strains. In the Miki fermentation model, Miki-A3 fermented well at 30 ℃ and decreased <em>Listeria monocytogenes</em> (Lm) count from 5 log to below the detection limit (< 3.3 log CFU/mL) in 24 h. In the fermented Miki stored at 10 ℃, the Lm count immediately decreased. These results indicate that Miki-A3 is a promising starter for rice-fermented beverages in terms of health functionality and hygiene.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 84-92"},"PeriodicalIF":3.7,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144580829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Water chestnut shell as a novel substrate for biosurfactant production: Enhanced oil recovery and soil decontamination 荸荠壳作为生产生物表面活性剂的新型底物：提高原油采收率和土壤净化

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-07-03 DOI: 10.1016/j.procbio.2025.07.001

Varsha Tripathi , Vivek Kumar Gaur , Natesan Manickam

{"title":"Water chestnut shell as a novel substrate for biosurfactant production: Enhanced oil recovery and soil decontamination","authors":"Varsha Tripathi , Vivek Kumar Gaur , Natesan Manickam","doi":"10.1016/j.procbio.2025.07.001","DOIUrl":"10.1016/j.procbio.2025.07.001","url":null,"abstract":"<div><div>In the realm of green chemistry, biosurfactants derived from fruit and vegetable wastes have garnered substantial interest due to their environmentally friendly applications. This study investigates the utilization of water chestnut shell powder (WCSP) as a novel substrate for biosurfactant production from <em>Pseudomonas aeruginosa</em> IITR110 and <em>Bacillus velezensis</em> IITR111. The extracted biosurfactants were identified as rhamnolipid and lipopeptide, with a yield of 1.18 g/L and 0.99 g/L, respectively. Their emulsification activities against crude oil demonstrated an E<sub>24</sub> value of 60.5 ± 1.8 % with rhamnolipid and 64.9 ± 1.4 % with lipopeptide. These biosurfactants exhibited robust stability across a wide range of pH 2.0–13.0, temperatures 4–120 ºC, and salinity levels 1–20 %, NaCl. The biosurfactants were employed in soil washing assay in which biosurfactants from IITR110 and IITR111 resulted in crude oil removal rates of 52.12 % and 58.03 %, respectively, outperforming the synthetic surfactant tween 20 (50.71 %). The sand pack column experiments revealed a substantially enhanced oil recovery percentage of 36 % for rhamnolipid and 43 % for lipopeptide. The novelty and significance of this study lie in the innovative use of water chestnut shell powder as an alternative substrate for biosurfactant production, thus presenting a sustainable and previously untapped approach.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 65-74"},"PeriodicalIF":3.7,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144549426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eco-friendly films from lemon peel pectin including essential oils for the sustainable tomato preservation 由柠檬皮果胶制成的环保薄膜，包括可持续保存番茄的精油

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-27 DOI: 10.1016/j.procbio.2025.06.018

Sirine Ben Dlala , Zeineb Mzoughi , Mariem Itaimi Dammak , Khaoula Khwaldia , Didier Le Cerf , Hamdi Ben Halima , Nicole Jaffrezic-Renault , Hafsa Korri-Youssoufi , Hatem Majdoub

{"title":"Eco-friendly films from lemon peel pectin including essential oils for the sustainable tomato preservation","authors":"Sirine Ben Dlala , Zeineb Mzoughi , Mariem Itaimi Dammak , Khaoula Khwaldia , Didier Le Cerf , Hamdi Ben Halima , Nicole Jaffrezic-Renault , Hafsa Korri-Youssoufi , Hatem Majdoub","doi":"10.1016/j.procbio.2025.06.018","DOIUrl":"10.1016/j.procbio.2025.06.018","url":null,"abstract":"<div><div>Several fruits and vegetables deteriorate within a few days, which poses a major challenge for their preservation and commercialization. To address this issue, this study reports on developing eco-friendly and safe films based on pectin extracted from lemon peel, enriched with essential oils, for a sustainable preservation of tomatoes. Pectin was extracted from <em>Citrus limon</em> peels using an innovative high-pressure treatment, achieving higher yields than conventional methods. The extracted pectin was characterized using advanced analytical techniques, FT-IR, Nuclear Magnetic Resonance (NMR), Size Exclusion Chromatography (SEC), and Gas Chromatography-Mass Spectrometry (GC-MS). Additionally, essential oils extracted from lemon and laurel were characterized by GC-MS to identify their active components. Pectin-chitosan-based films, with or without laurel and lemon essential oils, were prepared and evaluated for their mechanical properties, antimicrobial activity, moisture resistance, solubility, and biodegradability. These films were then tested for tomato preservation, demonstrating a significant extension of their shelf life beyond one month. Acting as a protective second skin, these films extend the freshness of fruits and vegetables by preventing microbial growth and reducing moisture loss. The results highlight the potential of these safe and eco-friendly films as a promising alternative to conventional plastic films.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 56-64"},"PeriodicalIF":3.7,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144535535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of novel dead-end filtration strategy for isolation of pullulan broth 蒲兰兰肉汤分离的新型终端过滤策略的开发

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-25 DOI: 10.1016/j.procbio.2025.06.016

Linyu Lu , Yiwen Li , Qingli Guan , Tingbin Zhao , Weifeng Cao , Changsheng Qiao

{"title":"Development of novel dead-end filtration strategy for isolation of pullulan broth","authors":"Linyu Lu , Yiwen Li , Qingli Guan , Tingbin Zhao , Weifeng Cao , Changsheng Qiao","doi":"10.1016/j.procbio.2025.06.016","DOIUrl":"10.1016/j.procbio.2025.06.016","url":null,"abstract":"<div><div>Pullulan, a natural extracellular polysaccharide, is widely used in food and related industries, cosmetics, and pharmaceuticals. The high viscosity of its fermentation broth poses significant challenges for efficient separation, making the process both difficult and time-consuming. In this study, cross-flow membrane filtration was first applied to separate a two-fold diluted broth (feed solution), where membranes with pore sizes ranging from 150 kDa to 0.8 μm rejected over 99 % of cells and approximately 50 % of pullulan. This phenomenon was attributed to higher shear forces increasing pullulan particle size in solution, thereby reducing its recovery rate. To address this limitation, a dead-end filtration strategy was developed using a 1–3 μm filtration medium with 1 % filter aid. During concentration-mode filtration, fouling mechanisms in the first stage showed indistinct differences between standard and intermediate blocking, while the second stage exhibited unclear distinctions between complete and intermediate blocking. A favorable membrane regeneration strategy was identified: sequential washing with fresh water followed by detergent powder. This method achieved 99.54 % biomass removal and a pullulan recovery rate of 90.67 %. Overall, the dead-end filtration strategy presents a novel approach for separating high-viscosity biopolymers, offering both efficiency and practicality for industrial applications.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 359-368"},"PeriodicalIF":3.7,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144490150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0