Process Biochemistry最新文献_第2页

A hybrid XGB-ANN model integrated with Lion Optimization Algorithm for improved prediction accuracy and lactic acid yield optimization 结合Lion优化算法的混合XGB-ANN模型，提高了预测精度和乳酸产率优化

IF 4 3区生物学

Process Biochemistry Pub Date : 2025-09-09 DOI: 10.1016/j.procbio.2025.09.005

Yang Shi , Xinnan Zheng , Xiaoxia Wang , Xiangyi Shi , Cheng Hou , Shizhuo Wang , Zipeng Huang , Zheng Shen

{"title":"A hybrid XGB-ANN model integrated with Lion Optimization Algorithm for improved prediction accuracy and lactic acid yield optimization","authors":"Yang Shi , Xinnan Zheng , Xiaoxia Wang , Xiangyi Shi , Cheng Hou , Shizhuo Wang , Zipeng Huang , Zheng Shen","doi":"10.1016/j.procbio.2025.09.005","DOIUrl":"10.1016/j.procbio.2025.09.005","url":null,"abstract":"<div><div>Machine learning (ML) has been widely applied in industrial fields in recent years. However, ML faces challenges in fermentation production due to complex features and limited data. To overcome the limitations of single models and improve the prediction accuracy and stability of lactic acid fermentation, we combined six classical ML models with Artificial Neural Networks (ANN) separately in a serial manner to construct the Classical-ANN (C-ANN) hybrid models. Meanwhile, implemented the Lion Optimization Algorithm (LOA) to systematically optimize critical fermentation parameters and conducted experimental validation. While single models showed moderate performance (Extreme Gradient Boosting (XGB) R²= 0.9197; ANN R²= 0.9295), C-ANN hybrids demonstrated superior accuracy across all metrics. The XGB-ANN model achieved exceptional performance (R²=0.9850), benefiting from XGB's robust feature selection and ANN's high-dimensional processing capabilities. SHAP analysis revealed fermentation time as the most critical yield determinant, followed by total volume and pH. Lion Optimization Algorithm parameters enabled a predicted yield of 81.21 %, with experimental validation showing only 4.72 % deviation from predictions. This study not only provides valuable assistance for optimization of fermentation production conditions in the short term but also offers an innovative and effective modeling approach for future research in this field.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"159 ","pages":"Pages 1-14"},"PeriodicalIF":4.0,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145061688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of rice straw pretreatment using microwave-assisted tetrahydrofuran-water systems through response surface methodology for improved sugar recovery 利用响应面法优化微波辅助四氢呋喃-水体系预处理水稻秸秆，以提高糖的回收率

IF 4 3区生物学

Process Biochemistry Pub Date : 2025-09-09 DOI: 10.1016/j.procbio.2025.09.003

Lakshana G. Nair, Pradeep Verma

{"title":"Optimization of rice straw pretreatment using microwave-assisted tetrahydrofuran-water systems through response surface methodology for improved sugar recovery","authors":"Lakshana G. Nair, Pradeep Verma","doi":"10.1016/j.procbio.2025.09.003","DOIUrl":"10.1016/j.procbio.2025.09.003","url":null,"abstract":"<div><div>A pretreatment process is indispensable for the reduction of biomass recalcitrance to enhance its hydrolytic bioconversion efficiency. A combination of Microwave heating and Tetrahydrofuran-water co-solvent systems was applied to Rice Straw (RS) to improve its sugar conversions. The current study employed a Central Composite Design-based Response Surface Methodology (CCD-RSM) to statistically optimize and study the interaction of different independent variables, like temperature, time, substrate ratio, and enzyme loading, on the total reducing sugar (TRS) yields on the RS pretreatment. At 100 °C and 40 min with 5.0 % (w/v) substrate concentration, the THF–water pretreatment system enabled the highest sugar recovery (76.60 %) from RS, achieved using an enzyme load of 200 U/g substrate. The significance of the pretreatment model was confirmed by ANOVA, with a p-value < 0.05 and an R<sup>2</sup> value of 0.97. The crystallinity indices calculated from FTIR and XRD were incorporated into a CCD-RSM model with three factors but were found to be non-significant. However, the reduction observed in the crystallinity indices of pretreated samples, concomitant with their higher sugar yields, indicates effective amorphization of cellulose. Furthermore, the detection of 12 distinct platform chemicals in the pretreatment hydrolysates also highlights the potential sustainability of this method for downstream bioconversions.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"158 ","pages":"Pages 203-218"},"PeriodicalIF":4.0,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioremediation of Sr2+ contamination based on microbially induced carbonate precipitation (MICP) simulated by Enterobacter sp. 基于模拟微生物诱导碳酸盐沉淀（MICP）的Sr2+污染生物修复

IF 4 3区生物学

Process Biochemistry Pub Date : 2025-09-07 DOI: 10.1016/j.procbio.2025.09.002

Zhimeng Su , Ting Chen , Fuchun Li , Xinyuan Guo , Lunxian Liu , Ximin Zhang , Ming Tang , Jing Tang , Xuelin Li , Yin Yi

{"title":"Bioremediation of Sr2+ contamination based on microbially induced carbonate precipitation (MICP) simulated by Enterobacter sp.","authors":"Zhimeng Su , Ting Chen , Fuchun Li , Xinyuan Guo , Lunxian Liu , Ximin Zhang , Ming Tang , Jing Tang , Xuelin Li , Yin Yi","doi":"10.1016/j.procbio.2025.09.002","DOIUrl":"10.1016/j.procbio.2025.09.002","url":null,"abstract":"<div><div>Microbially induced carbonate precipitation (MICP) has shown significant potential for Sr<sup>2+</sup> bioremediation in both laboratory and field settings. However, previous studies have primarily focused on urease-producing bacteria, with the potential of ammonifying bacteria remaining largely uninvestigated. This study explores the capacity of the ammonifying bacterium <em>Enterobacter</em> sp. JC-1 to promote carbonate mineral formation and enhance Sr<sup>2+</sup> removal under both static and shaking conditions. Solution chemistry analysis indicated that <em>Enterobacter</em> sp. JC-1 strain elevated both the pH value and HCO<sub>3</sub><sup>-</sup> concentration, inducing supersaturation with respect to calcite and strontianite, thereby promoting carbonate precipitation. Mineralogical characterization via XRD and SEM confirmed the formation of calcium-bearing strontianite with a microspherical morphology. During mineralization, Sr<sup>2+</sup> and Ca<sup>2+</sup> co-precipitated at molar ratios of approximately 1:1 (static conditions) and < 1:1 (shaking conditions), achieving Sr<sup>2+</sup> removal rate of 22 % and 33 %. Correlation analysis identified OD<sub>600</sub> and mineral mass as key factors influencing Sr<sup>2+</sup> removal. Comparative analysis with previous studies indicated that the Sr<sup>2+</sup> removal rate of JC-1 strain was lower than that of urease-producing bacteria, it exceeded that of urease-producing fungi. These findings highlight the potential of ammonifying bacteria as viable candidates for MICP-based bioremediation of heavy metals and radionuclides, thereby expanding the applicability of MICP in environmental engineering.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"158 ","pages":"Pages 194-202"},"PeriodicalIF":4.0,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photosynthetic activity and biocomponent production of Isochrysis galbana regulated by manipulated light quality 光质量调控下galbana等chrysis的光合活性和生物成分生产

IF 4 3区生物学

Process Biochemistry Pub Date : 2025-09-06 DOI: 10.1016/j.procbio.2025.09.004

Cuili Jin , Weiqi Tian , Zhixin Wu , Qing Liu , Xiaojian Zhou

{"title":"Photosynthetic activity and biocomponent production of Isochrysis galbana regulated by manipulated light quality","authors":"Cuili Jin , Weiqi Tian , Zhixin Wu , Qing Liu , Xiaojian Zhou","doi":"10.1016/j.procbio.2025.09.004","DOIUrl":"10.1016/j.procbio.2025.09.004","url":null,"abstract":"<div><div>To investigate how light quality regulates the biocomponent production and photosynthetic activities of <em>Isochrysis galbana</em>, monochromatic light of green (G), red (R), blue (B), and combinations of R and B (R: B=n: m, nRmB, n + m=7) were compared with achromatic white light (W) in this study. The results showed that B and blue-dominant light (1R6B-2R5B) reduced pigment concentrations, growth rates, and production of biomass and biocomponents compared to W, whereas G matched W. R had lower growth rate and biomass production than W but achieved the highest contents of carbohydrate, protein, and pigments. The combination 6R1B produced the highest biomass (480.28 mg/L), protein (99.19 mg/L), and lipid (213.44 mg/L), while 4R3B toped carbohydrate production (70.39 mg/L). G showed the highest energy dissipation and lowest photosynthetic parameters, whereas R had the highest electron transfer efficiency and pigment content. Mechanistically, red and blue combinations enabled the reaction center (RC) achieved the high electron transfer energy and low dissipated energy despite low absorption energy. Red-dominant combinations further offset the limit of absorption, boosting photosynthetic performance and biocomponent production. These findings clarify the regulatory role of light quality on algal photosynthesis and guide artificial irradiation for <em>I. galbana</em> biocomponent production.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"158 ","pages":"Pages 171-183"},"PeriodicalIF":4.0,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Micronutrients content directly impact cerulenin production by Sarocladium oryzae BRM 59907 微量元素的含量直接影响米瘟霉brm59907生产粟蓝素

IF 4 3区生物学

Process Biochemistry Pub Date : 2025-09-03 DOI: 10.1016/j.procbio.2025.08.014

João Rogério Borges de Amorim Rodrigues , Marcio Vinicius de Carvalho Barros Cortes , Elisa D.’Avila Costa Cavalcanti , Denise Maria Guimarães Freire

{"title":"Micronutrients content directly impact cerulenin production by Sarocladium oryzae BRM 59907","authors":"João Rogério Borges de Amorim Rodrigues , Marcio Vinicius de Carvalho Barros Cortes , Elisa D.’Avila Costa Cavalcanti , Denise Maria Guimarães Freire","doi":"10.1016/j.procbio.2025.08.014","DOIUrl":"10.1016/j.procbio.2025.08.014","url":null,"abstract":"<div><div>Fungal secondary metabolites, renewable compounds with diverse bioactivities, offer potential for medicines, colorants, and agrochemicals. Their biotechnological production bottlenecks, such as low production and variable yield, can be overcome through bioprocess engineering techniques and experimental designs. Cerulenin, a broad-spectrum antimicrobial compound produced by <em>Sarocladium oryzae</em>, can be obtained with higher yields and lower costs using these strategies. In this work, cerulenin production by <em>Sarocladium oryzae</em> BRM 59907 was optimized considering its micronutrient requirements. Plackett-Burman and Doehlert designs showed that magnesium supplementation inhibits cerulenin biosynthesis, while the addition of iron source stimulates it. Thus, it was possible to reach an optimal condition (20 g L<sup>−1</sup> glycerol, 5 g L<sup>−1</sup> glucose, 2.5 g L<sup>−1</sup> peptone, 0.025 g L<sup>−1</sup> NaCl, 0.029 g L<sup>−1</sup> FeCl<sub>3</sub>, 28 ºC and 150 rpm) with a reduction of 74 % in the cerulenin production cost considering only culture medium components from the original condition. Bioprocess scale-up in a bioreactor resulted in a cerulenin productivity of 1.39 mg L<sup>−1</sup> h<sup>−1</sup> in 72 h. This work presents the first bioprocess that efficiently utilizes glycerol as the primary carbon source for the secondary metabolite production. Magnesium supplementation increased cell growth but inhibited cerulenin production, while iron addition increased the concentration of cerulenin.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"158 ","pages":"Pages 184-193"},"PeriodicalIF":4.0,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimized production, purification and biochemical characterization of a novel glycerophosphodiesterase from a hyperthermophilic archaeon Pyrococcus abyssi 一种新型甘油磷酸二酯酶的优化生产、纯化和生化表征

IF 4 3区生物学

Process Biochemistry Pub Date : 2025-09-03 DOI: 10.1016/j.procbio.2025.09.001

Arshia Nazir , Muhammad Irfan , Naeem Rashid , Muhammad Sajjad

{"title":"Optimized production, purification and biochemical characterization of a novel glycerophosphodiesterase from a hyperthermophilic archaeon Pyrococcus abyssi","authors":"Arshia Nazir , Muhammad Irfan , Naeem Rashid , Muhammad Sajjad","doi":"10.1016/j.procbio.2025.09.001","DOIUrl":"10.1016/j.procbio.2025.09.001","url":null,"abstract":"<div><div>Organophosphate (OP) based pesticides, insecticides and nerve agents can cause nervous, respiratory, reproductive and hepatic problems in living organisms by serving as inhibitors of acetylcholinesterase. Recently, glycerophosphodiesterases (GDPDs) emerged as useful tools in degradation of organophosphate containing pesticides and nerve agents. The present work aims at recombinant production of a thermostable GDPD from <em>Pyrococcus abyssi</em> (Pa-GDPD) in <em>E. coli</em> under optimized cultural conditions. The actual molecular weight of the soluble recombinant enzyme was estimated to be ∼45 kDa with the help of gel filtration chromatography. Moreover, the optimum temperature and pH for Pa-GDPD’s activity were found to be 70 °C and 7.0, respectively. Activity of Pa-GDPD was found to be stimulated by Mn<sup>2 +</sup> followed by Fe<sup>2+</sup> and Mg<sup>2+</sup>. The recombinant enzyme exhibited its hydrolytic activity towards glycerophosphodiester as well as bis (<em>p</em>-nitrophenyl) phosphate (B<em>p</em>NPP) at elevated temperatures (50–90 °C). <em>K</em><sub>m</sub>, <em>V</em><sub>max</sub> and <em>K</em><sub>cat</sub> for B<em>p</em>NPP hydrolysis were determined to be 1 ± 0.145 mM, 88 ± 2.538 U/mg and 2.654 <span><math><mo>×</mo></math></span> 10<sup>3</sup> min<sup>−1</sup>, respectively<em>.</em> Thermoactivity, broad substrate spectrum and stability in organic solvents make recombinant Pa-GDPD an ideal candidate for organophosphate bioremediation.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"158 ","pages":"Pages 140-155"},"PeriodicalIF":4.0,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145004733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Major metabolites and biochemical basis of broad-spectrum antibacterial activity in marine-derived Streptomyces sp. EMB26 海源链霉菌EMB26的主要代谢产物及广谱抗菌活性的生化基础

IF 4 3区生物学

Process Biochemistry Pub Date : 2025-09-02 DOI: 10.1016/j.procbio.2025.08.016

Saniya Zaidi , Nitin Srivastava , Nikky Goel , Seema Sood , Sunil Kumar Khare

{"title":"Major metabolites and biochemical basis of broad-spectrum antibacterial activity in marine-derived Streptomyces sp. EMB26","authors":"Saniya Zaidi , Nitin Srivastava , Nikky Goel , Seema Sood , Sunil Kumar Khare","doi":"10.1016/j.procbio.2025.08.016","DOIUrl":"10.1016/j.procbio.2025.08.016","url":null,"abstract":"<div><div>As antimicrobial resistance becomes more prevalent, the need for new antibiotics against drug-resistant bacteria grows. Marine actinobacteria produce novel bioactive compounds with unique chemical structures that combat pathogens. This study investigated <em>Streptomyces</em> sp. EMB26, isolated from coastal Maharashtra, India, for its bactericidal activities against MDR bacteria, particularly MRSA. The crude extract strongly inhibited MRSA growth with an MIC of ∼2.5 mg/mL and caused bacterial cell disintegration, as seen in FESEM images. Fluorescent microscopy and cellular content leakage assays on MRSA cells confirmed <em>Streptomyces</em> sp. EMB26's bactericidal activity against MRSA. GC-MS profiling and molecular docking studies revealed that compound 7,9-Di-tert-butyl-1-oxaspiro(4,5)deca-6,9-diene-2,8-dione is primarily responsible for the bioactivity. The compound showed the highest binding affinity when docked against target proteins Fem A, MurB, MurE, and PBP2a, which are involved in MRSA's peptidoglycan cell wall biosynthesis. The findings suggest that <em>Streptomyces</em> sp. EMB26 is a promising marine actinomycete for developing an inhibitory drug against MDR bacteria.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"158 ","pages":"Pages 108-117"},"PeriodicalIF":4.0,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144989126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated optimization of EGFP production and direct recovery using stirred fluidized bed adsorption: Effects of culture and stirring conditions 搅拌流化床吸附生产和直接回收EGFP的综合优化：培养和搅拌条件的影响

IF 4 3区生物学

Process Biochemistry Pub Date : 2025-09-02 DOI: 10.1016/j.procbio.2025.08.017

Jyun-Liang Liu , Nguyen The Duc Hanh , Chanin Panjapornpon , Paweena Prapainainar , Kulpavee Jitapunkul , Chen‑Yaw Chiu , Bing-Lan Liu , Kuei Hsiang Chen , Yu-Kaung Chang

{"title":"Integrated optimization of EGFP production and direct recovery using stirred fluidized bed adsorption: Effects of culture and stirring conditions","authors":"Jyun-Liang Liu , Nguyen The Duc Hanh , Chanin Panjapornpon , Paweena Prapainainar , Kulpavee Jitapunkul , Chen‑Yaw Chiu , Bing-Lan Liu , Kuei Hsiang Chen , Yu-Kaung Chang","doi":"10.1016/j.procbio.2025.08.017","DOIUrl":"10.1016/j.procbio.2025.08.017","url":null,"abstract":"<div><div>An integrated approach was developed to optimize upstream production and downstream purification of enhanced green fluorescent protein (EGFP) in <em>Escherichia coli</em> BL21. Culture medium components were optimized using a statistical design of experiments, including a two-level Fractional Factorial Design (FFD) to screen key variables and a Central Composite Design (CCD) with Response Surface Methodology (RSM) to refine critical components. The optimal concentrations of glucose (4.215 g/L) and FeSO₄ (0.297 mg/L) resulted in a 333.1 % increase in EGFP production compared to a modified M9 medium. For purification, STREAMLINE DEAE ion-exchange chromatography was implemented in a stirred fluidized bed adsorption (SFBA) system designed to handle unclarified <em>E. coli</em> lysates (50 %, w/v). Stirring speed was optimized across the 0–200 rpm range, with 100–150 rpm providing the highest adsorption efficiency, recovery yield (∼87 %), and protein purity (purification factor ∼2.7). Unlike traditional packed-bed systems, the SFBA process enabled direct capture from crude lysates, improving throughput, reducing processing time, and enhancing scalability. This study demonstrates the effectiveness of coupling culture optimization with hydrodynamic tuning in SFBA for high-yield, clarification-free recovery of EGFP, offering a practical platform for producing and purifying recombinant proteins in industrial bioprocessing.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"158 ","pages":"Pages 156-170"},"PeriodicalIF":4.0,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Verification of mass transfer kinetics for chemical oxygen demand removal during the early stages of biogranulation 生物造粒初期化学需氧量去除传质动力学的验证

IF 4 3区生物学

Process Biochemistry Pub Date : 2025-09-02 DOI: 10.1016/j.procbio.2025.08.018

Farhan Mohd Pauzi , Khalida Muda , Mohamad Ali Fulazzaky

引用次数: 0

Statistical optimization of production and characterization of biosurfactant produced by Lactobacillus plantarum JBC5 strain in a ghee (clarified butter) medium: Assessment of its antimicrobial activity and stain removal property 植物乳杆菌JBC5菌株在酥油（澄清黄油）培养基中生产生物表面活性剂的统计优化及其特性：抑菌活性和去污性能的评估

IF 4 3区生物学

Process Biochemistry Pub Date : 2025-08-31 DOI: 10.1016/j.procbio.2025.08.015

Anushree Roy , Mojibur R. Khan , Ashis K. Mukherjee

{"title":"Statistical optimization of production and characterization of biosurfactant produced by Lactobacillus plantarum JBC5 strain in a ghee (clarified butter) medium: Assessment of its antimicrobial activity and stain removal property","authors":"Anushree Roy , Mojibur R. Khan , Ashis K. Mukherjee","doi":"10.1016/j.procbio.2025.08.015","DOIUrl":"10.1016/j.procbio.2025.08.015","url":null,"abstract":"<div><div>This investigation focused on optimizing and characterizing a biosurfactant derived from <em>Lactobacillus plantarum</em> JBC5, which was isolated from local curd. Ghee, a clarified butter that has not previously been explored as a substrate, was used for biosurfactant production. The Plackett-Burman factorial design identified significant parameters affecting bacterial growth and biosurfactant production, whereas response surface methodology (RSM) predicted optimal production at 3.2 g/L ± 0.08 g/L, which was experimentally validated at 3.1 ± 0.09 g/L.The column-purified biosurfactant demonstrated a critical micelle concentration of 100 mg/L, reducing water's surface tension to 34.0 ± 0.03 mN/m. The emulsification index (E<sub>24</sub>) of biosurfactants with edible oils was 59.8 %, making them a viable alternative to synthetic surfactants. Lipopeptide composition of biosurfactant was confirmed using nuclear magnetic resonance (NMR) and liquid chromatography-mas spectrometry analyses (LC-MS). Thermal analyses showed excellent heat stability as the crystallization and melting points were determined at 95.68°C and 276.72°C, respectively, suggesting its suitability for high-temperature industrial use. Combined with commercial facewash, the biosurfactant improved wash performance, as evidenced by increased post-wash solution absorbance. Antibacterial assay of biosurfactant showed bacteriostatic action against <em>Staphylococcus aureus</em>, with 61.3 % growth inhibition at 500 μg/mL. These findings position the biosurfactant as a promising candidate for cosmetic and industrial applications.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"158 ","pages":"Pages 129-139"},"PeriodicalIF":4.0,"publicationDate":"2025-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145004732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0