Process Biochemistry最新文献_第2页

Ultrasound-assisted intensified removal of dextran from sugarcane juice using dextranase 超声辅助葡聚糖酶强化脱除甘蔗汁中的葡聚糖

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-05-21 DOI: 10.1016/j.procbio.2025.05.012

Priya Yadav, Viraj N. Khasgiwale, Parag R. Gogate

{"title":"Ultrasound-assisted intensified removal of dextran from sugarcane juice using dextranase","authors":"Priya Yadav, Viraj N. Khasgiwale, Parag R. Gogate","doi":"10.1016/j.procbio.2025.05.012","DOIUrl":"10.1016/j.procbio.2025.05.012","url":null,"abstract":"<div><div>The present work demonstrates the effectiveness of dextranase-based ultrasound-assisted enzymatic treatment in reducing dextran content in sugarcane juice. Effect of various parameters such as enzyme loading (0.01 U – 0.07 U) treatment duration (5–20 min), temperature (40–70°C), pH (4−7), ultrasonic power (20–80 W), and duty cycle (20–50 %) on the dextran removal was studied. The scalability and efficiency of several ultrasonic reactors (US bath, hexagonal, and horn reactors) including the variation of frequencies (22, 33, and 48 kHz) using hexagonal reactor were also examined. Ultrasound-assisted treatment at 50°C, pH 5, a 10-min time, 30 % duty cycle, and 40 W power produced the best results for the highest dextran elimination (93 %). By contrast, only 63 % of the dextran was removed using the traditional method. Ultrasonic horn demonstrated the highest efficiency (93 %) attributed to higher power density followed by hexagonal reactor and ultrasonic baths (81 % and 72 %, respectively). Furthermore, the best results for dextran removal were obtained with single-frequency ultrasound at 22 kHz; whereas higher and combination frequencies resulted in decreased effectiveness because of wave interference and denaturation of the enzyme. The work has clearly demonstrated the effectiveness of ultrasound also elucidating the effect of reactor type and frequency for the first time.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 47-58"},"PeriodicalIF":3.7,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144134059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of the interaction between the de novo biosynthesis of vitamin B6 and its salvage pathway for efficient production of pyridoxine 维生素B6的新生生物合成及其有效生产吡哆醇的补救途径之间相互作用的调控

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-05-19 DOI: 10.1016/j.procbio.2025.05.010

Mengqing Lu, Haoran Sun, Zanwen Chen, Naiqiang Li

{"title":"Regulation of the interaction between the de novo biosynthesis of vitamin B6 and its salvage pathway for efficient production of pyridoxine","authors":"Mengqing Lu, Haoran Sun, Zanwen Chen, Naiqiang Li","doi":"10.1016/j.procbio.2025.05.010","DOIUrl":"10.1016/j.procbio.2025.05.010","url":null,"abstract":"<div><div>Vitamin B6, a micronutrient essential for maintaining healthy growth and normal physiological function, is a generic term for six interconvertible substances, of which pyridoxine hydrochloride is the most common commercial form, produced industrially by chemical processes using expensive and/or toxic chemicals. To date, attempts to engineer microorganisms to produce high-level of vitamin B6 have been unsuccessful due to the toxic intermediates and a lack of understanding of the vitamin B6 metabolism regulation. Here, for the first time, we proposed a two-step process for efficient pyridoxine production. A recombinant <em>Escherichia coli</em> was successfully engineered to produce 1406 mg/L of pyridoxal phosphate by interfering with only one or two key enzyme genes expression in the vitamin B6 salvage pathway. Pyridoxal phosphate was then efficiently converted to pyridoxine by another engineered <em>Escherichia coli</em> overexpressing cell membrane-anchored phosphatase YbhA and pyridoxal reductase PdxI, and 2296 mg/L (calculated as pyridoxine hydrochloride) of pyridoxine was achieved with a yield of 0.29 mol/mol (pyridoxine hydrochloride/pyridoxal phosphate) by shake flask cultivation. This achievement marked a significant advance in the biological synthesis of vitamin B6 and demonstrated an efficient vitamin B6 production strategy by reducing interference between the de novo synthesis pathway and the salvage pathway of vitamin B6.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 1-7"},"PeriodicalIF":3.7,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144105629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic protease-lipase treatment for enhanced blood stain removal from textiles: Process optimization and efficacy evaluation 协同蛋白酶-脂肪酶处理增强纺织品血渍去除：工艺优化和效果评价

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-05-18 DOI: 10.1016/j.procbio.2025.05.009

Mahammed Ilyas Khazi , Dilek Kut , Fakhra Liaqat , Elif Demirkan

{"title":"Synergistic protease-lipase treatment for enhanced blood stain removal from textiles: Process optimization and efficacy evaluation","authors":"Mahammed Ilyas Khazi , Dilek Kut , Fakhra Liaqat , Elif Demirkan","doi":"10.1016/j.procbio.2025.05.009","DOIUrl":"10.1016/j.procbio.2025.05.009","url":null,"abstract":"<div><div>This study investigates the synergistic effects of protease and lipase, produced from strains of <em>Bacillus subtilis</em> and <em>Bacillus cereus</em>, respectively, on blood stain removal from polyester and cotton fabrics. The effectiveness of enzyme - detergent combinations was evaluated using ΔE values as a quantitative measure of stain removal. Optimal culture conditions for enzyme production were determined using Response Surface Methodology (RSM) with Central Composite Design (CCD), identifying key variables such as temperature, pH, agitation, inoculum volume, and incubation time. Under optimized conditions, the enzymatic activity of protease and lipase reached 925.5 U/ml and 20.5 U/ml, respectively, representing a 2.4-fold and 3.1-fold increase compared to unoptimized conditions (376 U/ml for protease and 6.6 U/ml for lipase). Under optimal destaining conditions (pH 7.0, enzyme concentration of 1 mg/ml, and temperature of 60 °C), the combination of detergent, protease, and lipase achieved the highest ΔE values (58.42 ± 0.5 for polyester and 6.60 ± 0.5 for cotton fabric), signifying enhanced stain removal efficacy. Synergy between enzymes and detergents was highlighted, where detergent improved enzyme access to stain components, resulting in markedly improved cleaning efficiency compared to treatments with either enzyme or detergent alone. This study emphasizes the efficacy of enzyme-assisted formulations for sustainable textile cleaning applications.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 20-32"},"PeriodicalIF":3.7,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144116594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving citric acid production in Aspergillus niger by overexpression of citrate synthase with strong promoter 利用强启动子柠檬酸合成酶过表达提高黑曲霉柠檬酸产量

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-05-17 DOI: 10.1016/j.procbio.2025.05.005

Yiyang Tan , Yanhua Zhang , Jia Su , Hui Yu , Depei Wang , Xianli Xue

{"title":"Improving citric acid production in Aspergillus niger by overexpression of citrate synthase with strong promoter","authors":"Yiyang Tan , Yanhua Zhang , Jia Su , Hui Yu , Depei Wang , Xianli Xue","doi":"10.1016/j.procbio.2025.05.005","DOIUrl":"10.1016/j.procbio.2025.05.005","url":null,"abstract":"<div><div><em>Aspergillus niger</em> CGMCC10142, a strain widely used for industrial citric acid (CA) production, encodes four citrate synthase genes (<em>cs1-cs4</em>), yet their specific roles in CA biosynthesis remain poorly understood. We found that the transcript levels of the genes encoding mitochondrial citrate synthases, <em>cs3</em> or <em>cs4</em> were significantly higher than those encoding cytoplasmic citrate synthases <em>cs1</em> and <em>cs2</em> with 94.26–766.0 folds or 219.33–1782.25 folds, from 27.8 (<em>cs2</em>) to 49546.6 (<em>cs4</em>) at 24 h during CA fermentation. Given the naturally low transcription levels and cytoplasmic localization of <em>cs1</em> and <em>cs2</em>, we hypothesized that their overexpression would enhance CA production by increasing the CA available for export. Transformants overexpressing cytoplasmic <em>cs1</em> (T-c1–19) and <em>cs2</em> (T-c2–81) showed increases in CA production of 5.38 % and 18.39 %, respectively. In contrast, no significant change was observed in transformant overexpressing mitochondrial <em>cs3</em> (T-c3–11). The results showed a 6.8 % increase in acid production by strain T-c2–81, reaching 181.2 g/L at 56 h in 30 L bioreactors, compared to the starting strain of 169.6.g/L. qRT-PCR analysis of revealed that the relative expression levels of <em>cs2</em> in the T-c2–81 strain was 8.6 times higher than in the wild-type strain at 24 h. In addition, the expression levels of key genes <em>pfk</em>, <em>pc</em> and the citrate transporter <em>cexA</em> were significantly increased, ranging from 1.3 to 67 times than the control levels. This 6.8 % improvement of CA yield not only reduces unit fermentation costs but also represents a significant breakthrough in regulating intracellular metabolic flow.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 33-46"},"PeriodicalIF":3.7,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144124446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Designing salt-induced polymer-polymer biphasic systems for optimized extraction of Monascus ruber biopigments obtained by fermentation of xylose-based medium 设计盐诱导聚合物-聚合物双相体系，优化提取木糖基培养基发酵获得的红曲橡胶生物色素

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-05-16 DOI: 10.1016/j.procbio.2025.05.004

Nayara K.B. Lino , Gabriel L. Arruda , Felipe R. Lourenço , Valéria C. Santos-Ebinuma , Silvio S. da Silva , Júlio C. Santos , André M. Lopes

{"title":"Designing salt-induced polymer-polymer biphasic systems for optimized extraction of Monascus ruber biopigments obtained by fermentation of xylose-based medium","authors":"Nayara K.B. Lino , Gabriel L. Arruda , Felipe R. Lourenço , Valéria C. Santos-Ebinuma , Silvio S. da Silva , Júlio C. Santos , André M. Lopes","doi":"10.1016/j.procbio.2025.05.004","DOIUrl":"10.1016/j.procbio.2025.05.004","url":null,"abstract":"<div><div>The widespread use of synthetic colorants raises biosafety and environmental concerns, highlighting the need for natural alternatives like <em>Monascus</em> biopigments. These pigments possess antimicrobial and antioxidant properties, offering biotechnological and biopharmaceutical potential. Cost-effective biopigment production using xylose-rich hydrolysates from lignocellulosic biomass has been explored, but its sustainable downstream processing is crucial. Traditional liquid-liquid extraction with volatile organic compounds (VOCs) is harmful, prompting the use of eco-friendly aqueous biphasic systems (ABS) based on green chemistry principles. This study investigated the stability, partitioning, and purification of <em>Monascus ruber</em> biopigments (yellow, orange, and red) from fermented broth using ABS composed of polyethylene glycol (PEG), sodium polyacrylate (NaPA), and salts (MgSO<sub>4</sub> and CaCl<sub>2</sub>). Binodal curves showed MgSO<sub>4</sub> enhanced phase separation more effectively than CaCl<sub>2</sub>. Biopigments demonstrated high stability over 65 days and preferentially partitioned into the PEG-rich phase (<em>K</em><sub>Biopigments</sub> = 3–14), with recovery (<em>REC</em><sub>Top</sub>) values of 52–79 %. Optimal ABS conditions (18 % wt/wt PEG8000, 10 % wt/wt NaPA1200, and 0.3 M MgSO<sub>4</sub>) yielded biopigment <em>REC</em><sub>Top</sub> of 71–80 %, <em>K</em><sub>Biopigments</sub> of 12–15, and high selectivity over proteins (<em>S</em> = 14–19). Protein contaminants predominantly partitioned into the NaPA-rich phase, achieving a 10-fold purification. These findings support the stabilization and eco-friendly extraction of biopigments for bio-industrial applications.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 8-19"},"PeriodicalIF":3.7,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144105630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Continuous production of tagatose through isomerization of galactose using a tubular reactor filled with eggshell powder 用装满蛋壳粉的管状反应器通过半乳糖异构化连续生产塔格糖

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-05-15 DOI: 10.1016/j.procbio.2025.05.008

Shin Futamata, Yuichiro Onishi, Fumito Tani, Takashi Kobayashi

{"title":"Continuous production of tagatose through isomerization of galactose using a tubular reactor filled with eggshell powder","authors":"Shin Futamata, Yuichiro Onishi, Fumito Tani, Takashi Kobayashi","doi":"10.1016/j.procbio.2025.05.008","DOIUrl":"10.1016/j.procbio.2025.05.008","url":null,"abstract":"<div><div>Galactose solution was passed through a tubular reactor filled with eggshell powder, and isomerization of galactose to rare sugars was continuously performed under subcritical conditions at 120–150°C with the reaction time (RT) between 3–37 min. Isomerization produced rare sugars (tagatose, talose, and sorbose). At 135°C and RT= 6 min, the maximum yield of tagatose (14.5 %) was achieved. Compared to this result, the yield of tagatose was lower at 150°C or 120°C, and the yield of byproducts was also especially lower at 120°C. However, when RT was prolonged to 37 min at 120°C, the yield of tagatose improved to about 10 %, while the formation of byproducts was kept under control. Furthermore, during the continuous use of the reactor (up to 30 h), the yield of byproducts significantly decreased with increasing usage time. Therefore, it was suggested that continuous contact of eggshell with the reaction mixture may change properties of the powder and affect the isomerization behavior of galactose. Although the eggshell powder became light brown after use for the reaction, an apparent change of the surface could not be observed by scanning electron microscope.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"155 ","pages":"Pages 128-133"},"PeriodicalIF":3.7,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144090344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioactivity of autotrophic and mixotrophic Tetraselmis chuii (CCAP 66/21B) extracts against H2O2-mediated stress in A549 cells. 自养和混合养四叶鼠（ccap66 / 21b）提取物对h2o2介导的A549细胞应激的生物活性

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-05-15 DOI: 10.1016/j.procbio.2025.05.007

Thomas Conlon , Helen Herbert , Eva Campion , Nicolas Touzet

{"title":"Bioactivity of autotrophic and mixotrophic Tetraselmis chuii (CCAP 66/21B) extracts against H2O2-mediated stress in A549 cells.","authors":"Thomas Conlon , Helen Herbert , Eva Campion , Nicolas Touzet","doi":"10.1016/j.procbio.2025.05.007","DOIUrl":"10.1016/j.procbio.2025.05.007","url":null,"abstract":"<div><div>The trophic regime experienced by microalgae can influence their accumulation of antioxidant products such as phenolic compounds. This study investigated the antioxidant capacity and bioactivity of Dulbecco's phosphate-buffered saline (DPBS) extracts from the marine chlorophyte <em>Tetraselmis chuii</em> (CCAP 66/21B) cultivated under autotrophic and mixotrophic regimes. Compared to autotrophic conditions, mixotrophic cultivation significantly increased biomass yield (6.0-fold), soluble protein yield (5.7-fold) and Trolox-equivalent antioxidant capacity as measured by Folin-Ciocalteu (6.8-fold), DPPH (42.2-fold) and ABTS (15.3-fold) assays. For bioactivity, DPBS extracts (normalised by soluble protein content) were examined for their protective effects against hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)-mediated apoptosis in pulmonary carcinoma epithelial cells (A549). Both autotrophic and mixotrophic-derived extracts exhibited a dose-dependent response in H<sub>2</sub>O<sub>2</sub>-stressed A549 cells, with significant cytoprotection observed at higher concentrations (200 µg soluble protein mL<sup>−1</sup>), while lower concentrations were insufficient to mitigate oxidative damage and were associated with reduced cell viability. These effects involved modulation of apoptosis-related mRNA expression (<em>BCL2</em> and <em>BAX</em>). The bioactive content of both autotrophic and mixotrophic-derived extracts exhibited similar biological activity with properties that may be beneficial in pulmonary pharmacology. Importantly, mixotrophy significantly upregulated the bioactive content of <em>T. chuii</em> (CCAP 66/21B) compared to obligate autotrophy (34.2-fold accounting for soluble protein yield and biomass generation).</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"155 ","pages":"Pages 117-127"},"PeriodicalIF":3.7,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144084382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to 'Biosorption isotherm and composition of Amido black 10B decolorizing metal and salt tolerant bacterial consortium' [Process Biochem. 155 (2025) 60–69]

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-05-14 DOI: 10.1016/j.procbio.2025.05.002

Roselin Neihsial, Waniabha J. Lyngdoh, Imtiwala, Arvind Kumar Singh

引用次数: 0

Streamlined ultrasonication-assisted lipid extraction and direct transesterification of Rhodosporidium toruloides 1588 single-cell oil 流线型超声辅助下红孢子虫1588单细胞油的脂质提取和直接酯交换

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-05-14 DOI: 10.1016/j.procbio.2025.05.006

Luis Fernando Flores , Carlos S. Osorio-Gonzalez , Rahul Saini , Satinder Kaur Brar

{"title":"Streamlined ultrasonication-assisted lipid extraction and direct transesterification of Rhodosporidium toruloides 1588 single-cell oil","authors":"Luis Fernando Flores , Carlos S. Osorio-Gonzalez , Rahul Saini , Satinder Kaur Brar","doi":"10.1016/j.procbio.2025.05.006","DOIUrl":"10.1016/j.procbio.2025.05.006","url":null,"abstract":"<div><div><em>Rhodosporidium toruloides</em>-1588 efficiently utilizes various renewable residues for lipid synthesis, that potentially can be used for advanced drop-in biofuel production. However, to improve the feasibility, a streamlined sequential lipid extraction-transesterification process with minimal unit operations is essential. To enhance lipid extraction from <em>R. toruloides</em>-1588 fresh biomass, environmentally friendly solvents in combination with ultrasound-assisted extraction (UAE) were evaluated and compared to the highly used Folch-modified method. The results demonstrated a 291.45 % extraction efficiency in comparison to the reference method using methanol-UAE at the 1:1 ratio (broth removed: solvent added). Subsequent one- or two-step transesterification using KOH and H<sub>2</sub>SO<sub>4</sub> produced fatty acid methyl esters (FAME’s) with a similar distribution to the reference method being oleic, linoleic, palmitic, and stearic fatty acids, the most abundant. Thus, a potentially energy-saving method was developed by removing cell drying and thermochemical hydrolysis steps. Likewise, the proposed method decreases solvent consumption during the ultrasonication-assisted lipid extraction and direct transesterification of <em>R. toruloides</em>-1588 single-cell oil.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 1-9"},"PeriodicalIF":3.7,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144194940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-objective optimization of industrial batch Balhimycin antibiotic and fed-batch Lysine biochemical processes 工业批量巴尔希霉素抗生素和饲料批量赖氨酸生化工艺的多目标优化

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-05-13 DOI: 10.1016/j.procbio.2025.05.003

Swaprabha P. Patel , Ashish M. Gujarathi

{"title":"Multi-objective optimization of industrial batch Balhimycin antibiotic and fed-batch Lysine biochemical processes","authors":"Swaprabha P. Patel , Ashish M. Gujarathi","doi":"10.1016/j.procbio.2025.05.003","DOIUrl":"10.1016/j.procbio.2025.05.003","url":null,"abstract":"<div><div>Lysine is an essential amino acid, and its market value is USD 6.8 billion in 2023, and it is expected to double by 2033 worldwide. Balhimycin belongs to an important group of antibiotics with highly complex structures. With the utilization of advanced computational knowledge and new technologies, even a small improvement in productivity, yield, process time, or product quality can bring noticeable improvement in the production technology of biochemical processes. This study proposes a jumping gene adaptation of the elitist multi-objective differential evolution (Elitist MODE) algorithm. It is tested and applied successfully on the five benchmark test problems and two real-world problems, batch Balhimycin and fed-batch Lysine fermentation. Different constraint-handling techniques are used to test the performance of the proposed algorithm. The result obtained shows that the proposed algorithm can give a widespread Pareto front for both the test and industrial optimization problems. In batch Balhimycin fermentation, the optimal amount of glycerol required is not exceeding 59.84 g/L, and the optimal uptake of phosphate is also very low and doesn’t exceed 0.5 g/L, upon which product inhibition occurs. The uncertainty-specific study reveals that, with variation in upper bound of AMS concentration, product concentration varies significantly from 0.61 to 0.75 g/L.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"155 ","pages":"Pages 93-116"},"PeriodicalIF":3.7,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144070740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0