Process Biochemistry最新文献_第2页

Green utilization of sorghum straw by coupled hydrothermal pretreatment and cellulase hydrolysis for bacillomycin D production under fed-batch mode 通过水热预处理和纤维素酶水解耦合技术实现高粱秸秆的绿色利用，以饲料批量模式生产杆菌霉素 D

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2024-09-19 DOI: 10.1016/j.procbio.2024.09.014

引用次数: 0

Integrated in silico and in vitro evaluation of five anticancer peptides identified from Salvia hispanica 对从丹参中鉴定出的五种抗癌肽进行硅学和体外综合评估

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2024-09-18 DOI: 10.1016/j.procbio.2024.09.012

{"title":"Integrated in silico and in vitro evaluation of five anticancer peptides identified from Salvia hispanica","authors":"","doi":"10.1016/j.procbio.2024.09.012","DOIUrl":"10.1016/j.procbio.2024.09.012","url":null,"abstract":"<div><p>According to the World Health Organization, cancer is a leading cause of death worldwide. Several bioactive molecules, such as peptides, have been developed to adjuvate in cancer therapy. Previous evidence showed that the peptides KLKKNL, MLKSKR, KKYRVF, FRTKKK, and SVVAKAPVGKR, identified from a protein fraction of <em>S. hispanica</em> seeds, may serve as adjuvant therapy based on their physicochemical properties. Thus, this work aimed to evaluate the cytotoxic effect of these peptides on cancer cells through <em>in silico</em> and <em>in vitro</em> assays. Molecular dynamics simulations were performed to determine the interaction of the peptides with a cancer cell membrane model. Additionally, cell viability assays were performed to assess the effect of the peptides on MCF-7, Caco2, HepG2, DU145, HeLa, and hFB cell lines, and on erythrocytes. Both <em>in silico</em> and <em>in vitro</em> evaluations reported that KLKKNL, MLKSKR, KKYRVF, FRTKKK, and SVVAKAPVGKR interacted with the cancer cell membrane, significantly decreasing their viability. Molecular dynamics of KKYRVF exhibited a stable interaction with the cancer cell membrane model (-3.2 Kcal·mol-1), and experimentally showed selective cytotoxicity on the cancer cell lines. These findings support the continuous assessment of these peptides as adjuvants in cancer treatment for their potential as ingredients in functional foods or nutraceuticals.</p></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142274615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation, characterization and enhancement of intestinal iron absorption activity of β-casein phosphopeptides-iron chelate β-酪蛋白磷酸肽-铁螯合物的制备、表征及增强肠道铁吸收活性的作用

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2024-09-18 DOI: 10.1016/j.procbio.2024.09.013

{"title":"Preparation, characterization and enhancement of intestinal iron absorption activity of β-casein phosphopeptides-iron chelate","authors":"","doi":"10.1016/j.procbio.2024.09.013","DOIUrl":"10.1016/j.procbio.2024.09.013","url":null,"abstract":"<div><div>In this study, the β-casein phosphopeptides (β-CPPs) was enzymatically prepared from β-casein, followed by the preparation of β-casein phosphopeptides-iron chelate (β-CPPs-Fe). The results of Ultraviolet–Visible absorption spectroscopy (UV) and Fourier transform infrared spectroscopy (FTIR) indicated that the -COOH, O-H, N-H, and P-O-C of the β-CPPs may chelate with iron during the chelation. The amino acids involved in the chelation process were predominantly pSer, Tyr, Trp, and Phe. The scanning electron microscopy showed that due to β-CPPs combined with iron, the microsurface of β-CPPs-Fe displayed a predominantly loose porous structure. After the chelation with iron, the particle size distribution and zeta potential of β-CPPs-Fe increased. In vitro simulated gastrointestinal digestion revealed that β-CPPs-Fe demonstrated a significant increase in iron solubility compared to FeSO<sub>4</sub>·7 H<sub>2</sub>O (<em>p</em> < 0.05). At the endpoint of transport (180 min) in Caco-2 intestinal epithelial cell model, the amount of transferred iron for β-CPPs and β-CPPs-Fe increased by 30 % and 57 %, respectively, compared to the control group. Both β-CPPs and β-CPPs-Fe exhibited favorable effects on enhancing iron absorption, with the promotional impact of β-CPPs-Fe being notably more pronounced. The experimental findings establish a theoretical foundation for the industrial implementation of iron-fortified food products.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142316244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

To enhance the ex vivo expansion of CAR-NK-92 cells by regulating intracellular redox status 通过调节细胞内氧化还原状态增强 CAR-NK-92 细胞的体内外扩增

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2024-09-16 DOI: 10.1016/j.procbio.2024.09.010

{"title":"To enhance the ex vivo expansion of CAR-NK-92 cells by regulating intracellular redox status","authors":"","doi":"10.1016/j.procbio.2024.09.010","DOIUrl":"10.1016/j.procbio.2024.09.010","url":null,"abstract":"<div><p>Chimeric antigen receptor-Natural Killer-92 (CAR-NK-92) cell therapy has broad prospects as an effective cellular immunotherapy. Efficient CAR-NK-92 cell expansion <em>ex vivo</em> is crucial for its development and wide use. Unlike NK-92 cells, CAR-NK-92 cells need to maintain the stability of CAR expression during culture, besides keeping cell function. This work compared the growth and metabolism between NK-92 cells and CAR-NK-92 cells and found that the expansion efficiency of CAR-NK-92 cells was significantly lower than that of NK-92 cells. Meanwhile, the amino acid metabolism related to reducing agent production in CAR-NK-92 cells was weaker than in NK-92 cells, resulting in higher intracellular oxidation levels. The antioxidant N-acetylcysteine (NAC) was used to regulate the intracellular redox status of CAR-NK-92 cells. Under 1 mM NAC, the intracellular reactive oxygen species (ROS) level of CAR-NK-92 cells was down-regulated, and the cell expansion ability was improved. Furthermore, the addition of NAC has increased the levels of GSH and NADPH in CAR-NK-92 cells, elevated GSH/GSSG ratio and NADPH/NADP<sup>+</sup> ratio, enhanced the antioxidant capacity and mitochondrial function of cells, and promoted cell expansion. This study aims to promote CAR-NK-92 cell expansion <em>ex vivo</em> by regulating intracellular redox levels to facilitate its clinical application.</p></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142274616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic effects of Paenibacillus mucilaginosus and Penicillium pimiteouiense on the extraction of humic substances from lignite 粘土拟青霉和拟青霉对从褐煤中提取腐殖质的协同效应

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2024-09-15 DOI: 10.1016/j.procbio.2024.09.006

{"title":"Synergistic effects of Paenibacillus mucilaginosus and Penicillium pimiteouiense on the extraction of humic substances from lignite","authors":"","doi":"10.1016/j.procbio.2024.09.006","DOIUrl":"10.1016/j.procbio.2024.09.006","url":null,"abstract":"<div><p>Based on the common step of alkaline extraction-acid precipitation, lignite treatment techniques have been developed to improve extraction efficiency and yield of humic acid (HA). This study explored the combination of bacterium Paenibacillus mucilaginosus (P.m) LT1906 and fungus Penicillium pimiteouiense (P.p) LL2205 strains for pre- and post-treatment of lignite collected from eastern Inner Mongol to enhance HA and fulvic acid (FA) yield and plant growth-promoting activity. The lignite was subjected to P.m followed by HNO3 treatment, HNO3 followed by P.p treatment, and a combination of P.m, HNO3 and P.p treatments, then performed by alkaline extraction-acid precipitation technique. The results indicated that P.m pretreatment increased HA yield by 12.47 %, while P.p posttreatment significantly enhanced FA yield by 13.37 %. Combined bacterial and fungal treatments notably improved the total yields of HA and FA by 19.12 %. Structural analysis showed that P.m pretreatment caused the deformation on the lignite surface and the complete lignite oxidation and leaching reactions, thereby increasing the production of HA, yet had no impact on the elemental composition or chemical structure of HA. P.p posttreatment introduced oxygencontaining functional groups and reduced aromatic components through oxidative depolymerization of lignite, leading to increased FA production and HA seed germination-promoting activity.</p></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142274613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

UHPLC-Q-TOF-MS/MS identification of metabolites in cereal beverage ‘Borde’ and its anti-obesity efficacy in Caenorhabditis elegans model 超高效液相色谱-Q-TOF-MS/MS鉴定谷物饮料 "Borde "中的代谢物及其在秀丽隐杆线虫模型中的抗肥胖功效

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2024-09-13 DOI: 10.1016/j.procbio.2024.09.009

{"title":"UHPLC-Q-TOF-MS/MS identification of metabolites in cereal beverage ‘Borde’ and its anti-obesity efficacy in Caenorhabditis elegans model","authors":"","doi":"10.1016/j.procbio.2024.09.009","DOIUrl":"10.1016/j.procbio.2024.09.009","url":null,"abstract":"<div><p>This research explores the remarkable impact of fermented cereals such as sorghum, maize, and wheat with <em>Pediococcus acidilactici</em> WS07, a strain isolated from Ethiopia's traditional <em>borde</em> beverage. The fermented cereal extracts were thoroughly evaluated for antioxidant activity, inhibition of pancreatic lipase and α-glucosidase, metabolite identification through UHPLC-Q-TOF-MS/MS, and <em>in vivo</em> efficacy using the <em>C. elegans</em> model. The results revealed significant enhancements in the bioactive compounds of fermented cereals, demonstrating promising directions for obesity prevention strategies. Notably, the fermented sorghum extracts improved lipase (88.23 %) and α-glucosidase (85.62 %) inhibitory activities compared to its unfermented counterparts. The antioxidant properties of all fermented samples were confirmed through improved DPPH (67.77–71.86 %) and ABTS (59.91–65.49 %) scavenging activities. Fermentation also led to a notable increase in polyphenols and flavonoids, with detailed metabolite analysis revealing a dynamic shift in the composition of these bioactive compounds. Additionally, <em>C. elegans</em> indicates that fermented extracts extend lifespan, reduce lipid accumulation, and lower triglycerides, highlighting their potential as functional foods for health enhancement and obesity management. This study not only underscores the efficacy of <em>P. acidilactici</em> WS07 fermentation in transforming cereals into nutrient-rich functional foods but also provides insight into how microbial fermentation can unlock the health-promoting potential of traditional diets.</p></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142273967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design of a biolubricant by the enzymatic esterification of the free fatty acids from castor oil with neopentylglycol 通过酶法酯化蓖麻油中的游离脂肪酸和新戊二醇，设计一种生物润滑剂

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2024-09-12 DOI: 10.1016/j.procbio.2024.09.007

{"title":"Design of a biolubricant by the enzymatic esterification of the free fatty acids from castor oil with neopentylglycol","authors":"","doi":"10.1016/j.procbio.2024.09.007","DOIUrl":"10.1016/j.procbio.2024.09.007","url":null,"abstract":"<div><p>The optimization of the esterification of the free fatty acids (FFAs) from castor oil with neopentylglycol (NPG) catalyzed by the commercially immobilized lipase from <em>Thermomyces lanuginosus</em> (Lipozyme® TL IM) is herein reported. An 95 ± 3.3 % acid consumption was achieved at 55 °C, 6 wt% biocatalyst content and a substrates molar ratio (acid/alcohol)) of 2:1 after 24 h of reaction in a solvent-free medium. The addition of 1 M heptane seemed to lead to biocatalyst inactivation. The final product was composed of over 85 % of diesters of NPG, 5 % of monoesters of NPG and 5 % estolides (estolide number of 7), together to 2 % of unreacted FFAs. This product improved the feedstock viscosity index from 87.15 to 119.21 and the oxidative stability from 0.18 h to 24 h. It also exhibited a lower friction coefficient (0.0518 against 0.0592) and a lower wear scar diameter (117.99 against 136.78) than the commercial lubricant 15 W-50. Lipozyme® TL IM retained approximately half of its initial FFAs conversion after eight consecutive reaction cycles of 24 h.</p></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1359511324003040/pdfft?md5=30a7644048bf16b4ccf351cd431ea5b2&pid=1-s2.0-S1359511324003040-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142230124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of thermal treatment and secondary bonds on the storage stability of ready-to-eat sea cucumbers 热处理和二次粘合对即食海参储存稳定性的影响

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2024-09-11 DOI: 10.1016/j.procbio.2024.09.008

{"title":"Effect of thermal treatment and secondary bonds on the storage stability of ready-to-eat sea cucumbers","authors":"","doi":"10.1016/j.procbio.2024.09.008","DOIUrl":"10.1016/j.procbio.2024.09.008","url":null,"abstract":"<div><p>Ready-to-eat sea cucumbers (RSC) are highly sensitive to quality deterioration during storage at room temperature. The study investigated the anti-deterioration effects of high-pressure steam sterilization (HPSS) and high-temperature boiling (HTB) on RSC, as well as the impact of secondary bonds on HTB-treated RSC. The results indicated that there were no significant changes in the secondary structure following thermal treatment. Compared to day 0, the maximum thermal denaturation temperature of collagen in the control (CT) group decreased by 24.49℃, while decreases in the HPSS and HTB groups were 8.53℃ and 9.8℃, respectively. This confirmed that the stabilization of RSC could be enhanced through HPSS and HTB treatment during storage. When hydrogen bonds are destroyed, the texture properties of HTB-treated RSC significantly decrease, with hardness values dropping to 3.65±0.514 N and 2.41±0.615 N, springiness to 7.52±1.342 mm and 7.69±0.066 mm, and chewiness to 19.7±1.211 mj and 16.6±1.837 mj, respectively. Furthermore, the degradation of collagen fibers due to the breaking of hydrogen bonds prior to storage was more pronounced. These findings indicate that hydrogen bonds play a crucial role in maintaining the storage stability of RSC. These results offer a theoretical foundation and technical assistance for the processing and storage of RSC.</p></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142230123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extraction of polyphenols from Apocynum venetum leaves using customized deep eutectic solvents: Process optimization and antioxidant evaluation 使用定制的深共晶溶剂萃取鸦胆子叶中的多酚：工艺优化和抗氧化评估

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2024-09-11 DOI: 10.1016/j.procbio.2024.09.005

{"title":"Extraction of polyphenols from Apocynum venetum leaves using customized deep eutectic solvents: Process optimization and antioxidant evaluation","authors":"","doi":"10.1016/j.procbio.2024.09.005","DOIUrl":"10.1016/j.procbio.2024.09.005","url":null,"abstract":"<div><p>To encourage the commercial utilization of <em>A. venetum</em> polyphenols as bio-colorant on textile, five different combinations of deep eutectic solvents (DESs) were evaluated using a single-factor approach and the extraction of polyphenols from <em>A. venetum</em> leaves by ultrasound-assisted DESs was optimized. At the same time, the antioxidant activity of polyphenols prepared were investigated. The DESs composed of choline chloride and glycerol with a molar ratio of 1:2 and a water content of 20 % showed higher extraction performance than other DESs and traditional solvents (water, 60 % ethanol and 60 % methanol). With the most effective solvent the maximum yield 8.24 % of polyphenols was obtained under the optimal extraction conditions were as follows: the ratio of liquid volume (mL) to solid mass (g) is 49:1, extraction temperature of 77 ℃, and extraction time of 55 min. In addition, the polyphenols that have been separated and purified from the DESs extract exhibited antioxidant activities in three kinds of assays including 2,2-diphenyl-1-picrylhydrazyl stable free radical (DPPH), 2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulfonic) acid free radicals (ABTS) and hydroxyl radical (•OH) scavenging activity. Therefore, this paper demonstrates that DESs are effective and environmentally friendly solvents extraction of antioxidant polyphenols pigments from the leaves of <em>A. venetum</em>.</p></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142230125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel strategy for Klebsiella sp. to resist high salt and high phenol environmental stress 克雷伯氏菌抵抗高盐和高酚环境压力的新策略

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2024-09-11 DOI: 10.1016/j.procbio.2024.09.004

{"title":"A novel strategy for Klebsiella sp. to resist high salt and high phenol environmental stress","authors":"","doi":"10.1016/j.procbio.2024.09.004","DOIUrl":"10.1016/j.procbio.2024.09.004","url":null,"abstract":"<div><p>Isolating high-performance phenol degradation microorganisms with high salt tolerance and studying their resistance mechanisms are urgent issues. To address these issues, a typical bacteria (<em>Klebsiella</em> sp. YP-1) with high salt and phenol tolerance was isolated. Its strategies for resisting high salt and high phenol stress were studied. The results indicated that <em>Klebsiella</em> sp. YP-1 was able to degrade 1000 mg/L phenol within 44 h at 70 g/L NaCl, which was faster than most microorganisms reported in the literature. Lyxose secreted by <em>Klebsiella</em> sp. YP-1 played an important role on assisting <em>Klebsiella</em> sp. YP-1 to resist stress. Lyxose increased phenol degradation rate by microorganisms due to its protection on cell membrane. Quantum chemical calculation results indicated that lyxose was more likely attacked by free radical than cell membrane. In addition, lyxose could bind to the cell membrane through hydrogen bonds. Thus, lyxose prevented reactive oxygen species from harming cell membranes. Moreover, lyxose has broad protective effect on microbial cell membranes. This study provides a novel idea for microorganisms to resist oxidative stresses.</p></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142240523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0