超声增强的紫芪载长循环固体脂质纳米颗粒对卵巢癌中IL-6/STAT3信号通路的抑制作用

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry Pub Date : 2025-07-09 DOI:10.1016/j.procbio.2025.06.017

Wenjing Wang, Haibin Xi, Yi Ping

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引用次数: 0

摘要

本研究探索了一种新的卵巢癌联合治疗方法，即制备载紫芪固体脂质纳米颗粒（PT-SLNPs），并利用超声刺激提高其治疗效率。所制得的PT-SLNPs具有良好的物理化学性能，平均粒径为133.46 ± 24.21 nm，低多分散性指数（0.14 ± 0.02），高捕集效率（87.76 ± 0.5 %）。这项工作的独特之处在于PT-SLNPs与超声（US）的协同应用，显著增强了对人卵巢癌细胞A2780的细胞毒作用。此外，流式细胞术方法证实PT-SLNPs + US联合处理的细胞凋亡显著增加。从机制上讲，癌细胞暴露于PT-SLNPs + US通过抑制IL-6表达和抑制STAT3磷酸化，下调了肿瘤相关的关键促生存和增殖途径。这些发现突出了US辅助PT-SLNPs作为一种有希望的、毒性更低的卵巢癌化疗和放疗替代方案的创新潜力。

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Ultrasound-enhanced delivery of pterostilbene-loaded long-circulating solid lipid nanoparticles for the inhibition of IL-6/STAT3 signaling pathway in ovarian cancer

The current investigation explores a novel combinational approach for ovarian cancer treatment by formulating Pterostilbene-loaded solid lipid nanoparticles (PT-SLNPs) and enhancing their therapeutic efficiency using ultrasound stimulation. The formulated PT-SLNPs displays favorable physicochemical properties, with a mean particle size of 133.46 ± 24.21 nm, a low polydispersity index (0.14 ± 0.02), and high entrapment efficiency (87.76 ± 0.5 %). The uniqueness of this work is attributed to the synergistic application of PT-SLNPs and ultrasound (US), which significantly enhanced the cytotoxic effect against human ovarian cancer cell A2780. Further, flowcytometry method confirmed a considerable increase in apoptosis in cells treated with PT-SLNPs + US combination. Mechanistically, exposure of cancer cells to PT-SLNPs + US downregulated the key tumor associated pro-survival and proliferative pathways by suppressing IL-6 expression and inhibiting STAT3 phosphorylation. These findings highlight the innovative potential of US assisted PT-SLNPs as a promising, less toxic alternative to conventional chemotherapy and radiotherapy in ovarian cancer treatment.

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来源期刊

Process Biochemistry 生物-工程：化工

CiteScore

8.30

自引率

4.50%

发文量

374

审稿时长

53 days

期刊介绍： Process Biochemistry is an application-orientated research journal devoted to reporting advances with originality and novelty, in the science and technology of the processes involving bioactive molecules and living organisms. These processes concern the production of useful metabolites or materials, or the removal of toxic compounds using tools and methods of current biology and engineering. Its main areas of interest include novel bioprocesses and enabling technologies (such as nanobiotechnology, tissue engineering, directed evolution, metabolic engineering, systems biology, and synthetic biology) applicable in food (nutraceutical), healthcare (medical, pharmaceutical, cosmetic), energy (biofuels), environmental, and biorefinery industries and their underlying biological and engineering principles.