Process Biochemistry最新文献_第3页

Preparation, characterization and evaluation properties of a high-methoxyl pectin-iron (Ⅲ) from Actinidia arguta fruits 软枣猕猴桃果实高甲氧基果胶铁的制备、表征及性能评价（Ⅲ）

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-23 DOI: 10.1016/j.procbio.2025.06.013

Tienan Wang , Xinning Du , Yuhan Sui, Lina Chen

{"title":"Preparation, characterization and evaluation properties of a high-methoxyl pectin-iron (Ⅲ) from Actinidia arguta fruits","authors":"Tienan Wang , Xinning Du , Yuhan Sui, Lina Chen","doi":"10.1016/j.procbio.2025.06.013","DOIUrl":"10.1016/j.procbio.2025.06.013","url":null,"abstract":"<div><div>Iron deficiency anemia (IDA) was the most common nutritional disorder worldwide. As an effective and stable iron supplement, the physicochemical properties of iron complexes prepared using plant active ingredients have been extensively studied. In this study, a high methoxyl pectin AAP was extracted from the <em>Actinidia arguta</em> fruits. The synthesis process of AAP-iron (III) was optimized by single factor test and response surface methodology test. The physicochemical properties, structural characterization, and in vitro bioactivity were systematically investigated. Under the optimal synthesis conditions of pH 8.1, reaction time of 40 min, and temperature of 48 ℃, the iron content of AAP-iron (III) reached 26.62 ± 0.31 %. In addition, the -OH and -COOH groups in AAP coordinated with Fe<sup>3 +</sup> to form β-FeOOH groups. Compared with AAP, although the antioxidant capacity and α-glucosidase inhibitory activity of AAP-iron (III) were weakened, its α-amylase inhibitory activity was enhanced. In vitro digestion tests showed that AAP-iron (III) had a slow-release effect. Compared to FeSO<sub>4</sub>, AAP-iron (III) was more stable and not easily damaged by the simulated digestive solution. This study demonstrated that AAP-iron (III) was a promising new iron supplement.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 339-349"},"PeriodicalIF":3.7,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144481042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of exogenous combinations of fatty acids on the production of monensin by Streptomyces cinnamonensis 外源脂肪酸组合对肉桂链霉菌产莫能菌素的影响

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-23 DOI: 10.1016/j.procbio.2025.06.014

Shanfei Zhang , Zhenhua Wang , Dandan Tian , Yan Zhang , Chihe Sun , Qingming Hou , Jingle Liang , Qun Wu , Fubao Sun

{"title":"Effect of exogenous combinations of fatty acids on the production of monensin by Streptomyces cinnamonensis","authors":"Shanfei Zhang , Zhenhua Wang , Dandan Tian , Yan Zhang , Chihe Sun , Qingming Hou , Jingle Liang , Qun Wu , Fubao Sun","doi":"10.1016/j.procbio.2025.06.014","DOIUrl":"10.1016/j.procbio.2025.06.014","url":null,"abstract":"<div><div>Monensin is produced by the fermentation of <em>Streptomyces cinnamonensis</em>, which is in wide use in the agricultural and pharmaceutical industries. To improve the monensin yield, this study focused on evaluating the role of fatty acids in vegetable oil on the strain growth and secondary metabolism. Results showed that soybean oil was the most effective for monensin fermentation, followed by olive, peanut, camellia, corn, and linseed oils. Four fatty acids, oleic acid, linoleic acid, stearic acid, and palmitic acid, predominantly consisting of these vegetable oils, were observed to exert a positive effect on the monensin fermentation, thus resulting in the monensin titer increasing by 8-, 6-, 2-, and 3-fold, though far inferior to the soybean oil (10-fold). Optimization of fatty acid addition, comprising 1.38 % oleic, 2.78 % linoleic, 0.31 % stearic, and 0.50 % palmitic acid, elevated the monensin titer to 15.0 g/L. With a fed-batch fermentation in a 10-L tank, the fermentation reached 26.71 g/L of the monensin, above 10 % higher than that of the soybean oil. The suitable combination of fatty acids in vegetable oil was favorable for the microbial growth and monensin synthesis, which can shed light on the desirable application of different oil varieties in the industrial.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 350-358"},"PeriodicalIF":3.7,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144481040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polycaprolactone nanoparticles as a delivery vehicle for styrax liquidus: Therapeutic effects on ulcer and gastric cancer cells 聚己内酯纳米颗粒作为液体styrus的递送载体：对溃疡和胃癌细胞的治疗作用

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-23 DOI: 10.1016/j.procbio.2025.06.015

Eda Ülkeryıldız-Balçık , Hilal Sahin-Nadeem , Gulderen Cosgun , Dilara Konuk-Takma , Mahmut Yıldıztekin , Aleyna Gezen , Emine Yazici , Ece Oylumlu , Gamze Tanriover , Mehmet Torun

{"title":"Polycaprolactone nanoparticles as a delivery vehicle for styrax liquidus: Therapeutic effects on ulcer and gastric cancer cells","authors":"Eda Ülkeryıldız-Balçık , Hilal Sahin-Nadeem , Gulderen Cosgun , Dilara Konuk-Takma , Mahmut Yıldıztekin , Aleyna Gezen , Emine Yazici , Ece Oylumlu , Gamze Tanriover , Mehmet Torun","doi":"10.1016/j.procbio.2025.06.015","DOIUrl":"10.1016/j.procbio.2025.06.015","url":null,"abstract":"<div><div>Styrax liquidus (SL), a balsam from <em>Liquidambar orientalis</em> Mill., possesses high antioxidant, anti-inflammatory, anti-ulcer, and anti-cancer properties but is limited in food and medicine applications due to the immiscibility, high molecular size, and unable to pass the lipid membranes of the cells. Nano-delivery systems enhance bioavailability, stability, and controlled release of bioactive compounds. In this study, SL-loaded poly(ε-caprolactone) (PCL+SL) nanoparticles were manufactured by nanoprecipitation (NP) and flash nanoprecipitation (FNP) methods. Characterizations included size, PDI, zeta potential, morphology, encapsulation efficiency, and <em>in vitro</em> digestion release studies. SL concentration influenced nanoparticle properties, with FNP yielding efficient and size-controlled particles more readily. Spherical-smooth nanoparticles were produced within size ranges of 220–510 nm for NP and 171–383 nm for FNP, with narrow size distribution PDI (0.2 %). High encapsulation efficiencies and sustained gastric release (69–75 %) with low oral release (1.59–2.88 %) were observed. Optimal formulations showed a gastroprotective effect with high urease inhibition rates in range of 55.80 % - 54.69 % at 0.2 mg/mL concentration. An increased cytotoxicity effect of SL (IC<sub>50</sub> of 15.8 µg/mL, 81 % p < 0.0001) against AGS human gastric adenocarcinoma cells, followed by PCL+SL nanoparticles which have potential as “a delivery vehicle” in cancer treatment.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 42-55"},"PeriodicalIF":3.7,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144489595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anaerobic digestion of fats, oils, and greases (FOG) combined with carboxylic acids: Biogas kinetics, microbiome, and metabolic pathways 脂肪、油和油脂（FOG）与羧酸结合的厌氧消化：沼气动力学、微生物组和代谢途径

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-19 DOI: 10.1016/j.procbio.2025.06.011

Nandini Thakur , Ali S. Alkorbi , Mohammed Jalalah , Farid A. Harraz , Mostafa M. Koutb , El-Sayed Salama

{"title":"Anaerobic digestion of fats, oils, and greases (FOG) combined with carboxylic acids: Biogas kinetics, microbiome, and metabolic pathways","authors":"Nandini Thakur , Ali S. Alkorbi , Mohammed Jalalah , Farid A. Harraz , Mostafa M. Koutb , El-Sayed Salama","doi":"10.1016/j.procbio.2025.06.011","DOIUrl":"10.1016/j.procbio.2025.06.011","url":null,"abstract":"<div><div>The addition of carboxylic acids (C2 and C3) in anaerobic digestion (AD) can stimulate microbial growth and improve biogas production. Thus, C2 and C3 were added separately in two phases with an increasing fat, oil, and grease (FOG) concentration. The effects of C2 and C3 on biogas and volatile fatty acids (VFAs) production were studied. The microbial community shifts among bacteria and archaea were also examined, along with the metabolic pathways and functional enzymes. Biogas production during FOG digestion was increased by 1.69- and 1.47-fold upon the addition of C2 and C3, respectively, at the end of phase I. The accumulation of VFAs and long-chain fatty acids limited the biogas production in phase II. The <em>Clostridium</em> (28.8–48.3 %) and <em>Turicibacter</em> (20.1–8.7 %) were abundant in C2 and C3 amended FOG reactors. The addition of C3 also upsurged <em>Pseudigracilibacillus</em> (27.5 %) and <em>Thiopseudomonas</em> (9.08 %). <em>Methanosphaera</em> was the predominant archaea accounted for 79.1 % and 84.6 % in C2 and C3 amended reactors, respectively. The major predicted metabolic pathways were carbon metabolism (ko01200) and ABC transporters (ko02010). Glucosidase and coenzyme F<sub>420</sub> hydrogenase were the foremost bacterial and archaeal enzymes, respectively. Digestion of complex substrates (such as FOG) could be effectively enhanced by the addition of carboxylic acids.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 329-338"},"PeriodicalIF":3.7,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144481041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the interaction of preosteoblast cells with poly-L lysine surface-modified chitosan/hydroxyapatite scaffolds and their potential applications in bone tissue engineering 聚l赖氨酸表面修饰壳聚糖/羟基磷灰石支架与成骨前细胞的相互作用及其在骨组织工程中的潜在应用

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-19 DOI: 10.1016/j.procbio.2025.06.012

Tuğçe Kan Mutlu , Betül Mutlu Özçınar , İsmet Köksal , Rabia Çakır

{"title":"Investigating the interaction of preosteoblast cells with poly-L lysine surface-modified chitosan/hydroxyapatite scaffolds and their potential applications in bone tissue engineering","authors":"Tuğçe Kan Mutlu , Betül Mutlu Özçınar , İsmet Köksal , Rabia Çakır","doi":"10.1016/j.procbio.2025.06.012","DOIUrl":"10.1016/j.procbio.2025.06.012","url":null,"abstract":"<div><div>Bone is a multifunctional organ that undergoes constant structural and biological changes. In cases of damage due to trauma, cancer, infection or hormonal imbalances, medical intervention is required for bone regeneration. This study aims to develop a tissue scaffold that promotes bone tissue regeneration by enhancing cell adhesion, proliferation, and mineralization. For this purpose, tissue scaffolds with varying contents were produced using chitosan (Ch), hydroxyapatite (HA), and poly-L-lysine (PLL) as scaffold materials by freeze-drying method and characterized. In studies conducted to evaluate the biological activities of the scaffolds on MC3T3-E1 preosteoblast, the PLL-Ch/HA 1:2 scaffold exhibited approximately 20 % higher cell viability than the control on days 3 and 7 of extract analysis. In cells cultured on the scaffold, PLL-coated Ch/HA scaffolds showed a greater proliferative effect than uncoated Ch/HA scaffolds on day 7 of culture, resulting in a significant increase in cell viability. Furthermore, the observed increase in calcification and mineralization when cells were cultured on PLL-modified scaffolds could be attributed to PLL promoting cell adhesion and proliferation, resulting in increased calcium deposition. The surface modification of Ch/HA composite scaffolds with PLL has revealed optimal performance in bone tissue engineering due to their favorable performance.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 33-41"},"PeriodicalIF":3.7,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144489596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carbonate-enhanced cultivation of microalgae biofilm on the bovine serum albumin coated hollow fiber membranes 微藻生物膜在牛血清白蛋白包被中空纤维膜上的碳酸盐强化培养

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-17 DOI: 10.1016/j.procbio.2025.06.010

Jia Xin Yap , C.P. Leo , D.J.C. Chan , Nazlina Haiza Mohd Yasin

{"title":"Carbonate-enhanced cultivation of microalgae biofilm on the bovine serum albumin coated hollow fiber membranes","authors":"Jia Xin Yap , C.P. Leo , D.J.C. Chan , Nazlina Haiza Mohd Yasin","doi":"10.1016/j.procbio.2025.06.010","DOIUrl":"10.1016/j.procbio.2025.06.010","url":null,"abstract":"<div><div>Microalgae has been commercially grown to support the growth of biorefineries. Since microalgae are commonly grown using carbon dioxide from the air during biofilm cultivation, their efficiency in capturing carbon from a liquid medium remains unclear. In this study, sodium bicarbonate (NaHCO<sub>3</sub>) with varied concentrations was added as the additional carbon source during the cultivation of <em>Navicula incerta</em> on the bovine serum albumin (BSA)-coated hollow fiber membranes. <em>N. incerta</em>, a biofilm-forming diatom known for its surface adherence and environmental robustness, was selected for its suitability in the hollow fiber membrane cultivation system. The highest growth rate of 81.7 % was obtained by adding 8 g/L of NaHCO<sub>3</sub>, with a harvesting efficiency of 81.02 %. The carbon fixation rate of 375.45 ± 15.89 mg˖L<sup>−1</sup>˖d<sup>−1</sup> and carbon consumption efficiency of 58.91 ± 6.96 % were further attained. The chlorophyll content of <em>N. incerta</em> also increased from 2.61 ± 0.22–4.22 ± 0.28 mg/L. The microalgae biofilm on the BSA-coated PVDF hollow fiber membranes not only absorbed carbon dioxide from the air, but also removed the carbon source from the liquid.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 300-307"},"PeriodicalIF":3.7,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144322564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel spider-toxin-derived anti-gout peptide NCTX14 alleviated NLRP3-mediated pyroptosis in hyperuricemia 一种新型蜘蛛毒素衍生的抗痛风肽NCTX14减轻了nlrp3介导的高尿酸血症热亡

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-16 DOI: 10.1016/j.procbio.2025.06.009

Naixin Liu , Yixiang Liu , Junyuan Wang , Yuliu Yang , Yujing Ding , Xiaohan Sun , Mengfan Ma , Xin Yu , Shaoyang Zhang , Jing Tang , Xinwang Yang , Ying Wang

{"title":"A novel spider-toxin-derived anti-gout peptide NCTX14 alleviated NLRP3-mediated pyroptosis in hyperuricemia","authors":"Naixin Liu , Yixiang Liu , Junyuan Wang , Yuliu Yang , Yujing Ding , Xiaohan Sun , Mengfan Ma , Xin Yu , Shaoyang Zhang , Jing Tang , Xinwang Yang , Ying Wang","doi":"10.1016/j.procbio.2025.06.009","DOIUrl":"10.1016/j.procbio.2025.06.009","url":null,"abstract":"<div><div>Gout is a serious metabolic disorder mainly caused by high levels of serum uric acid (HUA). Due to the limitations of current treatments, it is important to investigate new urate-lowering compounds. In this study, a novel peptide, NCTX14 (CRPPVMLPKCEEFMK), was discovered within the venom gland of <em>Nephila clavata</em>. Research shows that NCTX14 doesn't exhibit cytotoxicity or hemolytic activity <em>in vitro</em> and is non-toxic when administered <em>in vivo</em>. This peptide effectively reduces serum uric acid levels and inhibits xanthine oxidase activity in mice with high uric acid levels. This is achieved through the downregulation of URAT1 and GLUT9, along with the upregulation of OAT1, ultimately enhancing uric acid excretion. Moreover, NCTX14 alleviates kidney damage by inhibiting the expression of inflammatory cytokines, such as IL-6, IL-1β, TNF-α. In HUA nephropathy, NCTX14 alleviates renal injury by inhibiting NLRP3 mediated pyroptosis injury. Additionally, NCTX14 demonstrates anti-inflammatory and pain-relieving effects in mice. The potential of NCTX14 in the treatment of gout suggests its practical application value as an anti-gout medicine candidate molecular.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 10-20"},"PeriodicalIF":3.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144297810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fast and green process to obtain astaxanthin-rich extracts from shrimp shell waste for dye sensitized solar cells 从虾壳废料中提取富含虾青素的染料敏化太阳能电池的快速绿色工艺

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-16 DOI: 10.1016/j.procbio.2025.06.006

Francesco Milano , Federica Mancarella , Livia Giotta , Ilaria Citro , Stefano Trocino , Giuseppe Calogero , Donatella Spadaro

{"title":"Fast and green process to obtain astaxanthin-rich extracts from shrimp shell waste for dye sensitized solar cells","authors":"Francesco Milano , Federica Mancarella , Livia Giotta , Ilaria Citro , Stefano Trocino , Giuseppe Calogero , Donatella Spadaro","doi":"10.1016/j.procbio.2025.06.006","DOIUrl":"10.1016/j.procbio.2025.06.006","url":null,"abstract":"<div><div>Aiming to address the critical need for renewable energy, this study explores the potential of astaxanthin-rich extracts from shrimp waste as natural sensitizers for dye-sensitized solar cells (DSSCs) using an eco-friendly and economically viable process. Photoconversion efficiencies (PCE) of astaxanthin-based DSSCs were evaluated through I-V measurements while charge transfer resistances (R<sub>CT</sub>) were assessed by electrochemical impedance spectroscopy. The sensitizer preparation involved extraction of astaxanthin from waste shrimp (<em>Aristaeomorpha foliacea</em>) cephalothoraxes, which were desiccated, finely milled and treated with ethyl acetate, to obtain a crude extract very rich in astaxanthin mainly present in esterified forms. The extraction process was fast and avoided extreme experimental conditions in terms of energy consumption, ultra-vacuum, high temperatures and use of toxic solvents. Direct application of the crude extract as sensitizer for DSSCs resulted in low PCE (0.09 %) and high R<sub>CT</sub>, due to the limited ability of esterified astaxanthin to interact with the TiO₂ layer. This limitation was overcome through saponification of the extract with methanolic NaOH, leading to free astaxanthin with high recovery yield and limited degradation. The saponified extract showed lower R<sub>CT</sub> and higher PCE (0.30 %). These results highlight the promising potential of suitably processed shrimp waste as a source of sensitizers for DSSCs.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 308-316"},"PeriodicalIF":3.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144322565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and optimization of a biological coating process based on chitosan from edible cricket to control Aspergillus niger-decay and weight loss of the Algerian dates cv. Deglet Nour 食用蟋蟀壳聚糖生物包衣工艺的开发与优化，以防治阿尔及利亚枣黑曲霉的腐烂和失重。Deglet努尔

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-16 DOI: 10.1016/j.procbio.2025.06.007

Imane Brahimi , Khadidja Allali , Abderrahmane Benadjila , Miyada Zamoum , Yacine Goudjal

{"title":"Development and optimization of a biological coating process based on chitosan from edible cricket to control Aspergillus niger-decay and weight loss of the Algerian dates cv. Deglet Nour","authors":"Imane Brahimi , Khadidja Allali , Abderrahmane Benadjila , Miyada Zamoum , Yacine Goudjal","doi":"10.1016/j.procbio.2025.06.007","DOIUrl":"10.1016/j.procbio.2025.06.007","url":null,"abstract":"<div><div>This study aims at reducing the post-harvest <em>Aspergillus niger</em> decay incidence of date palm through the biological coating process. To determine the most effective process, chitosan extracted from edible cricket (<em>Schistocerca gregaria</em>) combined with Arabic gum, Aloe vera gel or carboxymethyl cellulose was screened in comparison with the chemical fungicide Benomyl®. Similarly to the Benomyl®, coating dates with chitosan combined with Arabic gum significantly reduced the decay incidence (10 %) compared to untreated dates (100 %) and the weight losses were decreased from 6.02 % to 3.36 %. Coating dates with chitosan added to Arabic gum was selected for optimization using the Plackett-Burman and response surface methodologies approach. Acetic acid, gum Arabic and chitosan quantities were found to be the significant factors influencing the efficiency of the coating bioprocess. The optimized coating bioprocess was experimentally confirmed for the stored dates at 4 °C for 30 days and showed a complete elimination of <em>A</em>. <em>niger</em> decay incidence. The enhanced biological coating process based on chitosan added to gum can be proposed as a promising sustainable solution to reduce the abusive uses of chemical fungicides in <em>Deglet Nour</em> dates and to cut down weight losses during storage.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 21-32"},"PeriodicalIF":3.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144322761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Refining measurement techniques for N2H4, NH2OH, and NH4+-N in nitrogen-rich environments: Mitigating interference for accurate detection 改进富氮环境中N2H4、NH2OH和NH4+-N的测量技术：减轻干扰，实现准确检测

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-16 DOI: 10.1016/j.procbio.2025.06.008

Tugba Sari , Deniz Akgul

{"title":"Refining measurement techniques for N2H4, NH2OH, and NH4+-N in nitrogen-rich environments: Mitigating interference for accurate detection","authors":"Tugba Sari , Deniz Akgul","doi":"10.1016/j.procbio.2025.06.008","DOIUrl":"10.1016/j.procbio.2025.06.008","url":null,"abstract":"<div><div>In biological studies, several nitrogenous compounds interfere with each other’s measurements, which may result in unreliable results and misinterpretation. In this context, this study aims to refine the analytical techniques for N<sub>2</sub>H<sub>4</sub>, NH<sub>2</sub>OH, and NH<sub>4</sub><sup>+</sup>-N by eliminating interferences to obtain accurate data from the anammox system as a case study. Results indicated that NO<sub>2</sub><sup>-</sup>-N interferes with N<sub>2</sub>H<sub>4</sub> and NH<sub>2</sub>OH measurements, while N<sub>2</sub>H<sub>4</sub> and NH<sub>2</sub>OH interfere with NH<sub>4</sub><sup>+</sup>-N measurements. In N<sub>2</sub>H<sub>4</sub> determination, different NO<sub>2</sub><sup>-</sup>-N levels resulted in different utilization of sulfamic acid (SA) in terms of volume and concentration. The samples containing ≤ 2 mg/L NO<sub>2</sub><sup>-</sup>-N were pretreated with 400 µL 0.5 % SA to prevent NO<sub>2</sub><sup>-</sup>-N interference, while 10 % SA is required for NO<sub>2</sub><sup>-</sup>-N concentrations between 27.25 mg/L and 46.62 mg/L. The 10 % SA treatment also significantly mitigated NH<sub>2</sub>OH degradation in solutions, ensuring precise and steady results even in the presence of NO<sub>2</sub><sup>-</sup>-N. Adding 0.1 g of KIO<sub>3</sub> to 50 mL of samples containing N<sub>2</sub>H<sub>4</sub> (≤ 10 mg/L) produced findings most comparable to the control group in NH<sub>4</sub><sup>+</sup>-N tests. Furthermore, without any pretreatment, it is possible to measure NH<sub>4</sub><sup>+</sup>-N accurately if the NH<sub>2</sub>OH concentration is ≤ 0.01 mM. Overall, this study offers insightful data for further biological research in nitrogen-rich environments.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 317-328"},"PeriodicalIF":3.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144470500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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