Process Biochemistry最新文献

{"title":"Stabilization and conformational alteration of chymotrypsin upon losartan binding: Mechanistic insights from spectroscopy and molecular dynamics simulations","authors":"Saad Ali Alshehri ,&nbsp;Akhtar Atiya","doi":"10.1016/j.procbio.2026.01.014","DOIUrl":"10.1016/j.procbio.2026.01.014","url":null,"abstract":"<div><div>This study investigated the interaction between Chymotrypsin (CHY) and Losartan (LOS) using spectroscopic and computational techniques. UV–visible spectroscopy confirmed the formation of a stable CHY- (LOS) complex. Fluorescence quenching was static (<span><math><msub><mrow><mi>K</mi></mrow><mrow><mi>q</mi></mrow></msub></math></span> &gt; 2 × 10 ¹⁰ M⁻¹), with thermodynamic parameters (<span><math><msup><mrow><mo>∆</mo><mi>H</mi></mrow><mrow><mo>°</mo></mrow></msup></math></span> = −29.87 kJ mol⁻¹, <span><math><msup><mrow><mo>∆</mo><mi>S</mi></mrow><mrow><mo>°</mo></mrow></msup></math></span> = - 20.47 J mol⁻<em>¹</em> K⁻¹, <span><math><msup><mrow><mo>∆</mo><mi>G</mi></mrow><mrow><mo>°</mo></mrow></msup></math></span> = −23.78 to −23.38 kJ mol⁻¹ at 298–318 K) indicating spontaneous binding driven by hydrogen bonds and van der Waals forces. Enzyme kinetics demonstrated that increasing LOS concentrations (0–4 µM) resulted in a decrease in <span><math><msub><mrow><mi>V</mi></mrow><mrow><mi>max</mi></mrow></msub></math></span> (from 2.57 × 10⁻⁴ M s⁻¹ to 2.13 × 10⁻⁴ M s⁻<em>¹</em>) and a change in <span><math><msub><mrow><mi>K</mi></mrow><mrow><mi>m</mi></mrow></msub></math></span> (from 1.2¹ × 10⁻¹ mM to 0.91 × 10⁻¹ mM), suggesting uncompetitive inhibition. Circular dichroism (CD) and Fourier transform infrared (FTIR) spectroscopy provided further evidence of conformational changes, including a reduction in α-helix content and an increase in β-sheet content. Docking and molecular dynamics simulations (MD) supported these findings, revealing stable complex formation and enhanced thermal stability, with <span><math><msub><mrow><mi>T</mi></mrow><mrow><mi>m</mi></mrow></msub></math></span> increasing from 324 K to 332 K. These results suggest that LOS can engage in transient, non-covalent interactions with gastrointestinal proteases like CHY during pre-systemic exposure in the intestine. Such off-target binding may indirectly influence drug pharmacokinetics, affect digestive enzyme activity, and provide insights for developing protein-based carriers to improve the delivery of LOS and bioavailability.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"162 ","pages":"Pages 115-126"},"PeriodicalIF":4.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146039138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Degradation and control strategies for microplastics during aerobic composting: A review","authors":"Ping Liu,&nbsp;Hongli Huang,&nbsp;Xifeng Qin,&nbsp;Yuewei He,&nbsp;Jiahua Liu,&nbsp;Jiachao Zhang,&nbsp;Yuan Yang,&nbsp;Lihua Zhang","doi":"10.1016/j.procbio.2026.01.009","DOIUrl":"10.1016/j.procbio.2026.01.009","url":null,"abstract":"<div><div>Microplastics (MPs) pose a significant threat to the soil environment, food security, and human health. The application of organic fertilizers is recognized as a primary pathway for the introduction of MPs into agricultural soils. Research on the degradation of MPs during aerobic composting is in an early stage, and the degradation and control strategies for MPs during aerobic composting remain insufficiently researched. This paper systematically summarizes the sources, abundance, and occurrence characteristics of MPs in compost raw materials, including municipal sludge, livestock manure, kitchen waste, and household waste. It concludes the changes of MPs during composting and their degradation mechanisms: physical, chemical, and biological mechanisms. Furthermore, the paper summarizes the methods for enhancing the degradation of MPs, such as ultra-high-temperature composting, exogenous additives, and pretreatment technologies. Finally, the research directions are proposed in aspects of the detection methods of MPs, degradation mechanisms, enhancement of degradation approaches, environmental behavior of degradation products, and the risks of combined pollution by MPs. This paper aimed to provide insights for the control and management of MPs pollution.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"162 ","pages":"Pages 83-96"},"PeriodicalIF":4.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145980655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in protein substrate specificity during inactivation of different Alcalase formulations depends on inactivation conditions","authors":"Alan Portal D'Almeida ,&nbsp;Pedro Abellanas- Perez ,&nbsp;Tiago Lima de Albuquerque ,&nbsp;Luciana Rocha Barros Gonçalves ,&nbsp;Ivanildo José da Silva Junior ,&nbsp;Roberto Fernandez-Lafuente","doi":"10.1016/j.procbio.2026.01.003","DOIUrl":"10.1016/j.procbio.2026.01.003","url":null,"abstract":"<div><div>Free and carrageenan immobilized Alcalase preparations were incubated at 70ºC and pHs 5, 7, and 10, and their activities were monitored with the synthetic substrate Boc-<span>L</span>-Ala-4-nitrophenyl ester (NPA), casein, bovine serum albumin and hemoglobin. The inactivation profiles were fully different when followed using the different substrates. Stability was much lower when using NPA compared to the results obtained when using the substrate proteins. A great diversity could also be observed using the different protein substrates, however, the highest stability for the free and immobilized Alcalase was observed using different substrate proteins depending on the inactivation conditions, thus affecting the stabilization factors achieved by the immobilization (e.g., stabilization caused by the immobilization was negligible at pH 10 using HG, but very relevant at pH 5 using NPA). This was explained because the new enzyme conformations generated during inactivation, which depend on the inactivation conditions and enzyme formulations, have new protein substrate specificities.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"162 ","pages":"Pages 1-6"},"PeriodicalIF":4.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145908770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extraction of wool polypeptides using L-cysteine and protease: a study of the successive hydrolysis of the fibres’ regions","authors":"Clémence Marie-Renée Belbéoch,&nbsp;Jinsong Shen,&nbsp;Edward Smith","doi":"10.1016/j.procbio.2026.01.011","DOIUrl":"10.1016/j.procbio.2026.01.011","url":null,"abstract":"<div><div>Wool is a natural fibre with a diversity of applications in the textile industry. Its nature as a protein-rich fibre constituted of several distinct but interrelated regions, confers its unique mechanical properties. Due to the competition with superfine wools and the emergence of synthetic alternatives, coarser wools from the Northern hemisphere have suffered from a lack of valorising paths. Recently, studies successfully extracted wool polypeptides using sulphur-based reducing agents, such as sodium sulphite. These methods can separate fibres from textile blends for recycling purposes. In addition, the different wool protein types could be isolated to explore their post-consumer valorisation into new applications. In this study, the effectiveness of the amino acid, <span>L</span>-cysteine, as a sustainable reducing agent, combined with the activity of a protease on wool hydrolysis and proteins’ extraction was assessed, under different concentrations of <span>L</span>-cysteine and enzyme and duration of the reaction. Understanding the chronological order of the wool regions’ lysis during the extraction process is crucial for the control of specific parameters to selectively recover microfibrils or matrix proteins – which exhibit different protein structures. This selective recovery of the different wool proteins could support their regeneration into innovative materials, for applications in composites, packaging or construction.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"162 ","pages":"Pages 105-114"},"PeriodicalIF":4.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146038544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Moderate static magnetic fields induce higher coupling of the P-type H+-ATPase of fermentative yeast cells","authors":"Antonio Jesus Dorighetto Cogo ,&nbsp;Cristilane Macharete de Andrade ,&nbsp;Victor Haber Perez ,&nbsp;Oselys Rodriguez Justo ,&nbsp;Anna Lvovna Okorokova-Façanha ,&nbsp;Arnoldo Rocha Façanha","doi":"10.1016/j.procbio.2026.01.012","DOIUrl":"10.1016/j.procbio.2026.01.012","url":null,"abstract":"<div><div>An up-regulation of the proton pumping capacity of <em>S. cerevisiae</em> cells has previously been correlated with the magnetic field effects on alcoholic fermentation. Here, we further explore the mechanism behind this phenomenon by determining the ATP hydrolysis and H⁺ transport activities in the total membrane vesicles isolated by cell fractionation of fermentation yeast cells. The results revealed a contrasting effect as the hydrolysis of ATP decreased under the same magnetic field (10 mT) treatment that induced an increase in H⁺ uptake by cell membranes. This behavior can be interpreted as a more efficient fermentative metabolism that occurs during exposure to magnetic fields with moderate magnetic flux density, since higher transmembrane electrochemical H⁺ gradients are sustained at the expense of less ATP expenditure (energy consumption) as the membrane proton pump appears to be operating in a higher coupling state to maintain yeast metabolic activity. These findings seem particularly compelling from a biophysical standpoint, as they reveal a previously uncharacterized mechanism by which magnetic fields can induce metabolic reprogramming in yeast, potentially extending to other eukaryotic systems, by integrating modulations in energy transduction pathways and actin cytoskeleton dynamics.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"162 ","pages":"Pages 97-104"},"PeriodicalIF":4.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146038545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microaerobic bio-pretreatment of straw with fermentation broth as inoculum: Enhancing hydrolysis-acidification efficiency","authors":"Chuqiao Wang ,&nbsp;Xiaoqian Liu ,&nbsp;Jialong Pan ,&nbsp;Shan Huang ,&nbsp;Xizi Long ,&nbsp;Yuying Hu ,&nbsp;Jiajie Zhang","doi":"10.1016/j.procbio.2026.01.005","DOIUrl":"10.1016/j.procbio.2026.01.005","url":null,"abstract":"<div><div>Anaerobic digestion (AD) has been recognized as a promising technology for the disposal of agricultural residues, such as straw. Nonetheless, AD encounters a bottleneck of low hydrolysis rate. Microaerobic pretreatment significantly enhances straw AD efficiency, but its effectiveness depends on factors like pretreatment time and water content, with their interactions remaining unclear. Therefore, this study employed a response surface methodology (RSM) to investigate the interactions among pretreatment time, water content, and volatile fatty acids (VFAs) concentration in inoculum during the microaerobic pretreatment. The results showed that the highest hydrolysis-acidification efficiency was achieved with a pretreatment time of 10 days and a VFAs concentration of 10,000 mg/L, and there was a notable interaction between these two factors in the inoculum. Additionally, microbial community analysis showed that microaerobic pretreatment altered the microbial community structure to adapt to micro-oxygen environments, promoting the hydrolysis-acidification of straw. Specifically, <em>Proteobacteria</em>, <em>Firmicutes</em>, and <em>Actinobacteria</em> became the predominant acid-producing bacteria following microaerobic pretreatment. Within the fungus communities, <em>Aspergillus niger</em> and <em>Penicillium citrinum</em>, known for lignin degradation, increased by 4.4-fold and 1.2-fold, respectively. And the <em>Methanobacteria</em> became the main group in the archaeal communities. This paper presents a new perspective on the pretreatment of lignocellulose in commercial anaerobic reactors.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"162 ","pages":"Pages 127-135"},"PeriodicalIF":4.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146038546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glycerol as a carbon source to enhance apo-carotenes production in Azospirillum brasilense Cd","authors":"Claudia A. Contreras ,&nbsp;Vanesa Pelayo ,&nbsp;Carolina Garciglia ,&nbsp;Francisco J. Choix ,&nbsp;Guadalupe V. Nevárez-Moorillón ,&nbsp;Daniela Sánchez-Aldana ,&nbsp;Juan A. Ascacio-Valdés ,&nbsp;David Chávez-Flores ,&nbsp;Oskar A. Palacios","doi":"10.1016/j.procbio.2026.01.016","DOIUrl":"10.1016/j.procbio.2026.01.016","url":null,"abstract":"<div><div><em>Azospirillum brasilense</em> Cd is a non-photosynthetic pigmented bacterium, which has been mainly studied for its plant and microalgae growth-promoting effects. The bacterial growth conditions that enhance pigment production have been scarcely studied, with the effects of oxygen tension and some carbon sources on pigment production being explored. Moreover, the biochemical pathway for pigment production, its chemical characterization, and its biological function remain under debate. Therefore, the effects of different culture media, atmospheric conditions (air or biogas), carbon concentration, and the presence or absence of light on pigment production by <em>A. brasilense</em> were evaluated. Moreover, the chemical characterization, antioxidant activity, and changes in gene expression by <em>A. brasilense</em> under the growth conditions that yielded the best pigment production were evaluated. Culture media with glycerol as the carbon source under an air atmosphere yielded better pigment production than culture media with gluconate and/or malic acid as the carbon source. The presence of air and light increases twofold pigment production in <em>A. brasilense</em> Cd. The higher glycerol concentration tested (64 mg·L⁻¹) under air and light was the best growth condition for biomass and pigment production, yielding 36.1 ± 1.1 mg·g⁻¹ dry weight of the pigment, which is, to date, the highest pigment production reported for this bacterium. The chemical characterization of the orange-red pigment revealed structures corresponding to C30 aporacotenes, specifically diapolycopene-dial and diaponeurosporenic acid. Microarray analysis results enable elucidation of the possible metabolic pathway, via the squalene route, used by <em>A. brasilense</em> Cd to metabolize glycerol as an energy source and for pigment production.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"162 ","pages":"Pages 159-169"},"PeriodicalIF":4.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146078951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis, characterization, antifungal and SERS analysis of mycosynthesized silver nanoparticles against Aspergillus parasiticus","authors":"Anam Ijaz,&nbsp;Muhammad Rizwan Javed","doi":"10.1016/j.procbio.2026.01.017","DOIUrl":"10.1016/j.procbio.2026.01.017","url":null,"abstract":"<div><div>Myco-nanotechnology has gained significant momentum in the current decade in producing high yield of key enzymes and secondary metabolites as natural reducing agents to reduce metal ions into nanoparticles. In the current study, synthesis of AgNPs has been evaluated by CRISPR-modified (M-AgNPs) and its parent <em>Aspergillus niger</em> (P-AgNPs) strains. These AgNPs were characterized by UV-Vis spectroscopy, FTIR, TEM, SEM, DLS, and XRD. The analyses revealed that the synthesized AgNPs have characteristic peak at 420 nm, associated respective functional groups, spherical in shape, having average diameters of 8 and 14 nm, respectively, with crystalline structure. Furthermore, antifungal activities of AgNPs against aflatoxin producing <em>Aspergillus parasiticus</em> were evaluated, followed by the determination of MIC and MFC<em>.</em> The MICs of M-AgNPs and P-AgNPs were 18.75 and 37.2 μg/mL, while their MFCs were 75 and 150 μg/mL, respectively. The biochemical changes in fungal biomolecules were also investigated by SERS, showing an increasing pattern with higher concentrations of AgNPs. The M-AgNPs exhibited higher antifungal activity against <em>A. parasiticus</em> compared with P-AgNPs. This study provides, for the first time, a detailed SERS analysis of AgNPs against <em>A. parasiticus</em>, which may help to elucidate the action mechanism of antifungal agents and their potential to control fungal infections.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"162 ","pages":"Pages 170-182"},"PeriodicalIF":4.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146079051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Encapsulation promotes the anti-osteoporosis effects of vitamin D3 with enhanced bioavailability and activation efficiency","authors":"Weifeng Hu ,&nbsp;Min Zhu ,&nbsp;Yihan Wen ,&nbsp;Yi Guo ,&nbsp;Pei Qiao","doi":"10.1016/j.procbio.2026.01.006","DOIUrl":"10.1016/j.procbio.2026.01.006","url":null,"abstract":"<div><div>Vitamin D3 (Cholecalciferol, Chc) plays a critical role in the prevention and treatment of osteoporosis. However, Chc’s clinical and nutritional applications are substantially limited by poor aqueous solubility, low stability, and inadequate bioavailability. In this study, we developed a nano-micellar delivery system by encapsulating Chc with disodium glycyrrhizin (Na₂GA) to form Chc-Na₂GA micelles, aiming to overcome these limitations. The resulting formulation exhibited markedly improved solubility, enhanced physicochemical stability, increased bioavailability, and a higher hepatic accumulation rate. Importantly, Chc-Na₂GA treatment significantly enhanced anti-osteoporotic efficacy, accompanied by the upregulation of key osteogenesis-related genes, including <em>bmp2a</em>, <em>bump4</em>, <em>col2a</em>, <em>runx2a</em>, and <em>sp7</em>. Structural analysis revealed that Chc was present in an amorphous state within the micelles, stabilized primarily through hydrogen bonding and Pi-alkyl interactions. Overall, this study demonstrates that Na₂GA-based nano-micellization is an effective strategy to improve the delivery and biological efficacy of hydrophobic vitamin D3, highlighting its potential significance for pharmaceutical and food applications in osteoporosis management.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"162 ","pages":"Pages 24-31"},"PeriodicalIF":4.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145980661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The influence and correlation analysis of environmental factors and saturation index on the calcium carbonate precipitation induced by carbonic anhydrase-producing bacteria","authors":"Jiahua Zhu ,&nbsp;Jing Sun ,&nbsp;Nan Hu ,&nbsp;Zhihui Yang ,&nbsp;Aishu Li ,&nbsp;Shunchao Yao ,&nbsp;Guangyue Li","doi":"10.1016/j.procbio.2026.01.010","DOIUrl":"10.1016/j.procbio.2026.01.010","url":null,"abstract":"<div><div>Microbially induced carbonate precipitation (MICP) has garnered widespread attention as a promising biomineralization technology. However, existing studies predominantly focus on single-factor effects, lacking systematic analysis of multi-factor interactions and mineralization kinetics. This study systematically investigated the dynamic influences of environmental factors on the mineralization process and precipitation characteristics induced by a carbonic anhydrase-producing strain. Through correlation analysis, a multi-parameter heatmap was constructed to reveal biomineralization kinetic patterns and clarify the role of the saturation index (SI) in linking microbial metabolism with precipitation characteristics. The results indicated that both precipitation yield and SI exhibit dynamic variations during biomineralization. Under the optimized conditions of 1.0 OD₆₀₀ bacterial solution (BS), 0.2 M cementation solution (CS), 30°C and pH 10, precipitation yield and SI peaked at 3.85 g/L and 3.96, respectively. Higher BS concentration, temperature, pH and SI, combined with a lower CS concentration, favored the formation of regularly shaped calcite. Conversely, lower BS/CS concentrations, pH and SI significantly increased the particle size. Notably, CS concentration and pH exerted more pronounced effects on precipitation characteristics and SI. This study systematically elucidates the kinetic mechanisms of biomineralization and coupling regulatory relationship of environmental factors, providing a theoretical basis for predicting, regulating and optimizing the mineralization process.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"162 ","pages":"Pages 136-148"},"PeriodicalIF":4.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146039137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}