Process Biochemistry最新文献_第3页

Multi-objective optimization of industrial batch Balhimycin antibiotic and fed-batch Lysine biochemical processes 工业批量巴尔希霉素抗生素和饲料批量赖氨酸生化工艺的多目标优化

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-05-13 DOI: 10.1016/j.procbio.2025.05.003

Swaprabha P. Patel , Ashish M. Gujarathi

{"title":"Multi-objective optimization of industrial batch Balhimycin antibiotic and fed-batch Lysine biochemical processes","authors":"Swaprabha P. Patel , Ashish M. Gujarathi","doi":"10.1016/j.procbio.2025.05.003","DOIUrl":"10.1016/j.procbio.2025.05.003","url":null,"abstract":"<div><div>Lysine is an essential amino acid, and its market value is USD 6.8 billion in 2023, and it is expected to double by 2033 worldwide. Balhimycin belongs to an important group of antibiotics with highly complex structures. With the utilization of advanced computational knowledge and new technologies, even a small improvement in productivity, yield, process time, or product quality can bring noticeable improvement in the production technology of biochemical processes. This study proposes a jumping gene adaptation of the elitist multi-objective differential evolution (Elitist MODE) algorithm. It is tested and applied successfully on the five benchmark test problems and two real-world problems, batch Balhimycin and fed-batch Lysine fermentation. Different constraint-handling techniques are used to test the performance of the proposed algorithm. The result obtained shows that the proposed algorithm can give a widespread Pareto front for both the test and industrial optimization problems. In batch Balhimycin fermentation, the optimal amount of glycerol required is not exceeding 59.84 g/L, and the optimal uptake of phosphate is also very low and doesn’t exceed 0.5 g/L, upon which product inhibition occurs. The uncertainty-specific study reveals that, with variation in upper bound of AMS concentration, product concentration varies significantly from 0.61 to 0.75 g/L.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"155 ","pages":"Pages 93-116"},"PeriodicalIF":3.7,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144070740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the structure and properties of humic acids from Yunnan lignite transformed by Penicillium ortum MJ51 青霉MJ51转化云南褐煤腐植酸的结构与性质评价

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-05-03 DOI: 10.1016/j.procbio.2025.04.024

Shiying Li , Fang Li , Peipei Li , Yanjun Yue , Jinfang Tan , Xiaolei Jie , Wei Zhou , Yanlai Han

{"title":"Evaluation of the structure and properties of humic acids from Yunnan lignite transformed by Penicillium ortum MJ51","authors":"Shiying Li , Fang Li , Peipei Li , Yanjun Yue , Jinfang Tan , Xiaolei Jie , Wei Zhou , Yanlai Han","doi":"10.1016/j.procbio.2025.04.024","DOIUrl":"10.1016/j.procbio.2025.04.024","url":null,"abstract":"<div><div>Biotransformation of low-rank coal into humic acids (HAs) has received increasing attention. In this study, <em>Penicillium ortum</em> MJ51, isolated from coal, was used to transform Yunnan lignite into HAs. Differences in the yield, structure and properties were evaluated for HAs produced from the biodegradation of nitric acid pretreated-lignite (MHA), and those produced via chemical extraction from raw lignite (CHA<sub>1</sub>) and nitric acid pretreated-lignite (CHA<sub>2</sub>). The results show that with the progression of degradation time, the MHA yield gradually increased and peaked on day 12 at 62.39 %, which was 199.60 % and 40.68 % higher than that of CHA<sub>1</sub> and CHA<sub>2</sub>, respectively. Meanwhile, the contents of macro-micro nutrients and active groups (including carbonyl, carboxyl, methoxy, and N-alkyl), as well as the anti-flocculation ability and aliphaticity of MHA also gradually increased. However, the contents of C, H, N, heavy metals and ash, particle size, relative molecular weight, aromaticity, and hydrophobicity of MHA gradually decreased. On days 12–16 of degradation, the MHA had lower heavy metals, rare earth elements and ash content, higher macro-micro nutrients content, lower relative molecular weight and aromaticity, higher anti-flocculation ability and bioactivity than those extracted via the chemical method, making it suitable for agricultural applications.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"155 ","pages":"Pages 70-83"},"PeriodicalIF":3.7,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143921980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stable biofilm formation via nutrition regulation for enhancing toluene removal in gas–solid fluidized-bed bioreactors 通过营养调节形成稳定的生物膜以增强气固流化床生物反应器中甲苯的去除

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-05-02 DOI: 10.1016/j.procbio.2025.05.001

Yangdan Fang , Lichao Lu , Guangquan Ou , Qinqin Zhu , Zhuqiu Sun , Ziyu Liu , Marvin Yeung , Jinying Xi

{"title":"Stable biofilm formation via nutrition regulation for enhancing toluene removal in gas–solid fluidized-bed bioreactors","authors":"Yangdan Fang , Lichao Lu , Guangquan Ou , Qinqin Zhu , Zhuqiu Sun , Ziyu Liu , Marvin Yeung , Jinying Xi","doi":"10.1016/j.procbio.2025.05.001","DOIUrl":"10.1016/j.procbio.2025.05.001","url":null,"abstract":"<div><div>Gas-solid fluidized-bed bioreactors (FBRs), an innovative equipment for gaseous volatile organic compound (VOC) treatment, are used to realize efficient removal of VOCs characterized by large volumes and low concentrations and avoid clogging due to excessive biofilm accumulation. However, difficulties in biomass accumulation caused by stressful environmental pressures, especially shear force, limit their development. In this study, two laboratory-scale FBRs were built, and toluene removal was nearly twiced with saturated relative humidity and adequate nutrition supplement. Accelerated biofilm formation, activated microbial metabolism, enhanced mechanical strength, and surface smoothness were also observed. Nutrition regulation optimized cell synthesis and extracellular polymeric substance secretion through alternation of the accumulation of elements, especially C/N and Ca, which increased by 84 % and 112 %, respectively. The biofilm loss was reduced by 50–75 % under compression and collision conditions, indicating the promoted biofilm stability under shear force caused by extrusion and collision between particles. With the initial drawbacks partially mitigated, nutrient regulation during FBR operation was found to be useful. This study provides theoretical support for further applications of FBRs and efficient treatment of gaseous VOCs with large gas volumes and low concentrations.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"155 ","pages":"Pages 84-91"},"PeriodicalIF":3.7,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143921981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Purification, characterization, and toxicity of antioxidative peptides from ultrasonicated chickpea (Cicer arietinum L.) protein hydrolysate 超声鹰嘴豆（Cicer arietinum L.）蛋白水解物中抗氧化肽的纯化、表征和毒性研究

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-04-29 DOI: 10.1016/j.procbio.2025.04.026

Deia Tawalbeh , Fisal Ahmad , Shantanu Sengupta , Muhammad Hussin Alu’datt , Wan Amir Nizam Wan Ahmad , Mannur Ismail Shaik , Nanda Kumar Yellapu , Norizah Mhd Sarbon

{"title":"Purification, characterization, and toxicity of antioxidative peptides from ultrasonicated chickpea (Cicer arietinum L.) protein hydrolysate","authors":"Deia Tawalbeh , Fisal Ahmad , Shantanu Sengupta , Muhammad Hussin Alu’datt , Wan Amir Nizam Wan Ahmad , Mannur Ismail Shaik , Nanda Kumar Yellapu , Norizah Mhd Sarbon","doi":"10.1016/j.procbio.2025.04.026","DOIUrl":"10.1016/j.procbio.2025.04.026","url":null,"abstract":"<div><div>This study aimed to assess the purification, characterization and toxicity of antioxidative peptides from ultrasonicated chickpea protein hydrolysate (UCPH) by enzymatic hydrolysis. The UCPH was purified sequentially using ultrafiltration and fast protein liquid chromatography (FPLC). The < 3 kDa fraction (UCPH-1) from ultrafiltration was further purified using FPLC after demonstrating strong lipid-peroxidation inhibition activity (ferric thiocyanate (FTC): 49.54 % and thiobarbituric acid reactive species (TBARS): 55.42 %). The F6 fraction from FPLC was found to be an F6 peptide (UCPH-1-F6) with an amino acid sequence of MLRVYLR, which demonstrated a greater lipid-peroxidation inhibitory activity (FTC: 55.62 % and TBARS: 62.20 %). The UCPH-1-F6 peptide also showed potent antioxidant properties. In addition, the molecular docking studies showed that the MLRVYLR exhibited hydrogen-bonds interaction with 1,1-diphenyl-2-picrylhydrazyl (DPPH) by CDOKER. The MLRVYLR showed no toxicity properties to the brine shrimp lethality, which indicates it is a safe peptide. These results suggest that the purified peptide derived from ultrasound chickpea protein hydrolysate has potential antioxidant properties and can be used in functional food, nutraceuticals, and the pharmaceutical industry.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"154 ","pages":"Pages 220-233"},"PeriodicalIF":3.7,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143892276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comprehensive review of large-scale extraction and purification of food-derived bioactive phenolic components 食品源性生物活性酚类成分大规模提取纯化技术综述

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-04-29 DOI: 10.1016/j.procbio.2025.04.027

Eman Shawky , Lutfun Nahar , Sarah M. Nassief , Satyajit D. Sarker , Reham S. Ibrahim

{"title":"A comprehensive review of large-scale extraction and purification of food-derived bioactive phenolic components","authors":"Eman Shawky , Lutfun Nahar , Sarah M. Nassief , Satyajit D. Sarker , Reham S. Ibrahim","doi":"10.1016/j.procbio.2025.04.027","DOIUrl":"10.1016/j.procbio.2025.04.027","url":null,"abstract":"<div><div>As the market for functional foods and nutraceuticals grows, traditional extraction methods often fall short of meeting large-scale production needs. This review analyzes recent advancements in large-scale extraction and purification methods for phenolic compounds, addressing the industrial demand for high-quality phenolic-rich products. It explores innovative technologies such as ultrasound-assisted extraction, microwave-assisted extraction, and pressurized liquid extraction. The challenges of scaling these technologies from laboratory to industrial scale are discussed, including maintaining bioactivity, optimizing process parameters, and ensuring cost-effectiveness. The review also covers advanced purification techniques like membrane filtration, adsorption processes, and advanced chromatographic methods. Additionally, the review highlights the environmental and economic aspects of large-scale phenolic extraction, emphasizing sustainable practices, such as eco-friendly solvents and waste valorization. It provides insights into the latest trends in process design and optimization, including computational modeling and process intensification strategies. Finally, the review identifies key areas for future research and development.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"154 ","pages":"Pages 196-219"},"PeriodicalIF":3.7,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143892277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sustainable pectin extraction: Navigating industrial challenges and opportunities with fruit by-products – A review 可持续果胶提取：导航工业挑战和机遇与水果副产品-综述

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-04-28 DOI: 10.1016/j.procbio.2025.04.025

Durga Gomathi Arumuganainar , Deepa Lakshmi Ramesh , Prakash Pandurangan , Swetha Sunkar , Stanley Abraham , Antony V. Samrot , Ravi Sainandhini , A. Thirumurugan , Meivelu Moovendhan

{"title":"Sustainable pectin extraction: Navigating industrial challenges and opportunities with fruit by-products – A review","authors":"Durga Gomathi Arumuganainar , Deepa Lakshmi Ramesh , Prakash Pandurangan , Swetha Sunkar , Stanley Abraham , Antony V. Samrot , Ravi Sainandhini , A. Thirumurugan , Meivelu Moovendhan","doi":"10.1016/j.procbio.2025.04.025","DOIUrl":"10.1016/j.procbio.2025.04.025","url":null,"abstract":"<div><div>Fruit by-products from the food processing sector are produced in large quantities and have great potential as sustainable sources of pectin, a bioactive polysaccharide used extensively in the food, pharmaceutical and nutraceutical industries. With a focus on sustainability, this paper investigates the various uses, characterisation and extraction of pectin derived from fruit waste. These processes include traditional acid-based extraction methods, as well as emerging technologies such as microwave-assisted and enzyme-aided approaches. Critical obstacles to scaling up the pectin extraction process, including feedstock availability, financial concerns, and quality variances, are also covered in the review. Using unconventional extraction techniques is highlighted as a viable option to increase pectin production while lessening environmental effects. Insights into pectin's functional qualities, such as its gelling, thickening, and emulsifying capabilities, further demonstrate its versatility in industrial applications. Despite the challenges of industrial-scale implementation, transforming fruit by-products into pectin offers a viable strategy for waste reduction and the creation of valuable commodities. This assessment promotes a circular economy in the fruit processing industry by supporting efficient extraction methods and creative solutions to industrial problems.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"154 ","pages":"Pages 234-245"},"PeriodicalIF":3.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143911742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ginkgo leaf derived amphiphilic and biocompatible carbon dots for sterilization and MRSA biofilm destruction 银杏叶衍生的两亲性和生物相容性碳点用于杀菌和MRSA生物膜破坏

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-04-27 DOI: 10.1016/j.procbio.2025.04.023

Peili Li , Gan Zhao , Long Meng , Jumen Wei , Junhua Chen , Yunhe Xu , Da Zhang , Xiang Ke , Zirong Li

{"title":"Ginkgo leaf derived amphiphilic and biocompatible carbon dots for sterilization and MRSA biofilm destruction","authors":"Peili Li , Gan Zhao , Long Meng , Jumen Wei , Junhua Chen , Yunhe Xu , Da Zhang , Xiang Ke , Zirong Li","doi":"10.1016/j.procbio.2025.04.023","DOIUrl":"10.1016/j.procbio.2025.04.023","url":null,"abstract":"<div><div>Multidrug-resistant (MDR) bacterial infections, typified by methicillin-resistant <em>Staphylococcus aureus</em> (MRSA), have become a severe global challenge to healthcare systems. In response, antibacterial carbon dots (CDs) have attracted growing interest in the fight against MRSA. In this study, nitrogen self-doped ginkgo leaf (GL) carbon dots (GLCDs) were synthesized through a facile one-step solvothermal method combined with extraction technology. The prepared GLCDs are spherical nanoparticles with a crystalline structure and carry a positive charge of + 7.62 mV. The minimum inhibitory concentration (MIC) of GLCDs against MRSA was found to be 250 μg/mL. Notably, at a concentration of 750 μg/mL, GLCDs could completely eliminate 1 × 10<sup>6</sup> MRSA within 240 minutes and effectively suppress MRSA biofilm formation in a dose-dependent manner. The antimicrobial mechanisms involve electrostatic interaction enabling penetration into MRSA, disruption of the cell structure leading to intracellular component leakage, induction of oxidative stress, and inhibition of antioxidant enzymes and respiratory function. Moreover, GLCDs demonstrated excellent biocompatibility in hemolysis and <em>in vitro</em> cytotoxicity tests. This research presents a novel natural-plant-based antimicrobial nanomaterial, providing valuable insights into the rational utilization of natural biomass to combat MRSA infections.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"154 ","pages":"Pages 172-183"},"PeriodicalIF":3.7,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143887302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sterilized and raw tequila vinasse as a medium for ethanol production via S. cerevisiae and K. marxianus: Estimation and comparison of its kinetic parameters 以酿酒酵母和马氏酵母生产乙醇为培养基的灭菌生龙舌兰酒asse：动力学参数的估计和比较

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-04-25 DOI: 10.1016/j.procbio.2025.04.019

Juan Alberto Regalado-Aguirre , Fernando López-Caamal , Héctor Hernández-Escoto , María Cristina Del Rincón-Castro , Juan Antonio Ramírez-Vázquez , Glenda Edith Cea-Barcia

{"title":"Sterilized and raw tequila vinasse as a medium for ethanol production via S. cerevisiae and K. marxianus: Estimation and comparison of its kinetic parameters","authors":"Juan Alberto Regalado-Aguirre , Fernando López-Caamal , Héctor Hernández-Escoto , María Cristina Del Rincón-Castro , Juan Antonio Ramírez-Vázquez , Glenda Edith Cea-Barcia","doi":"10.1016/j.procbio.2025.04.019","DOIUrl":"10.1016/j.procbio.2025.04.019","url":null,"abstract":"<div><div>Tequila production generates vinasse as wastewater that may be valorized to produce ethanol. To assess vinasse’s value as a source of biofuel in circular bioeconomy, culture media were formulated with sterilized and raw vinasse using glucose as model substrate and two conventional yeasts, for comparison. One of them is <em>S. cerevisiae,</em> and the other strain, <em>K. marxianus,</em> was isolated from a tequila vinasse. In sterilized vinasse, results suggest that both yeasts activate secondary metabolic pathways as stress response to inhibitory compounds and salt, being <em>S. cerevisiae</em> the strain that showed better ethanol production (1.3 g L<sup>−1</sup> h<sup>−1</sup>). However, such a remark is opposite when <em>K. marxianus</em> was subjected to ALE strategy to improve its tolerance to vinasse’s stressors. Adapted <em>K. marxianus</em> enhanced its ethanol production (1.2 g L<sup>−1</sup> h<sup>−1</sup>) as compared to <em>S. cerevisiae</em> (1.1 g L<sup>−1</sup> h<sup>−1</sup>) in raw vinasse. To quantify how media and strains affect ethanol production, a dynamic mathematical model was formulated, and its parameter values were fitted for each scenario. A comparison of such values shows that ALE strategy adequates the reaction rate to the media, agreeing with experimental data. Both the maximum reaction rate and the half velocity constant increased after ALE, when considering a Monod reaction rate; thus, leading to a quicker reaction and to a reaction rate that saturates at larger substrate concentrations. In turn, the parameters values with both yeasts are similar to each other. These results confirm that tequila vinasse medium is suitable for ethanol production.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"154 ","pages":"Pages 119-128"},"PeriodicalIF":3.7,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143883136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In situ insertion of one or two types of hydroxy-rich noncanonical amino acids into one protein 将一种或两种富含羟基的非规范氨基酸原位插入到一个蛋白质中

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-04-25 DOI: 10.1016/j.procbio.2025.04.022

Xuanhe Fan , Yumei Liu , Zhenya Chen

{"title":"In situ insertion of one or two types of hydroxy-rich noncanonical amino acids into one protein","authors":"Xuanhe Fan , Yumei Liu , Zhenya Chen","doi":"10.1016/j.procbio.2025.04.022","DOIUrl":"10.1016/j.procbio.2025.04.022","url":null,"abstract":"<div><div>Noncanonical amino acids (ncAAs) possess unique properties owing to their distinct functional groups. The predominant method for inserting ncAAs into proteins is genetic code expansion, which mimics the natural translation process and requires exogenous ncAAs supplementation. However, microbial cells often lack specific transporters for ncAAs uptake across the cell membrane and do not recognise them as essential nutrients, leading to limited insertion efficiency. To address these challenges, we developed an <em>in situ</em> insertion method to enhance ncAAs insertion efficiency and explored the feasibility of co-inserting two types of ncAAs into a single protein. Initially, orthogonal translation systems specific to the hydroxy-rich ncAAs 5-hydroxytryptophan (5-HTP) and 4-hydroxyisoleucine (4-HiL) were constructed and introduced into microbial cells. Subsequently, the biosynthetic pathways for 5-HTP and 4-HiL were engineered in <em>Escherichia coli</em>, which harboured the corresponding orthogonal translation systems. Furthermore, a co-insertion strategy was developed based on the dual use of UGA and UAG codons. <em>In situ</em> co-insertion of 5-HTP and 4-HiL into one protein was achieved by introducing biosynthetic pathways and orthogonal translation systems for 5-HTP and 4-HiL into the same microbial cell. These findings establish a robust framework for the precise and scalable insertion of ncAAs in advanced protein design.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"154 ","pages":"Pages 129-142"},"PeriodicalIF":3.7,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143883138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on the antibacterial activity and mechanism of prodigiosin against Methicillin-resistant Staphylococcus aureus: Insights from metabolomic and transcriptomic analyses 对耐甲氧西林金黄色葡萄球菌的抑菌活性及机制研究：代谢组学和转录组学分析的见解

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-04-25 DOI: 10.1016/j.procbio.2025.04.021

Xin Wang, Zongyu Zhang, Jiacheng Zhao, Xinying Liu, Jinghua Wang, Zongyong Lv, Qianxi Song, Dongliang Hua, Guangfan Meng, Jie Zhang, Jing Zhang

{"title":"Study on the antibacterial activity and mechanism of prodigiosin against Methicillin-resistant Staphylococcus aureus: Insights from metabolomic and transcriptomic analyses","authors":"Xin Wang, Zongyu Zhang, Jiacheng Zhao, Xinying Liu, Jinghua Wang, Zongyong Lv, Qianxi Song, Dongliang Hua, Guangfan Meng, Jie Zhang, Jing Zhang","doi":"10.1016/j.procbio.2025.04.021","DOIUrl":"10.1016/j.procbio.2025.04.021","url":null,"abstract":"<div><div>Methicillin-Resistant <em>Staphylococcus aureus</em> (MRSA) is one of the pathogens that cause food poisoning. Prodigiosin (PG) is a kind of microbial secondary metabolites with antibacterial, anti-inflammatory, anti-tumor and other biological activities. In the present study, PG as the natural metabolite of <em>Serratia marcescens</em> was found to have the significant bacteriostatic activity against MRSA undergoing division and proliferation. It can damage cell walls, cell membranes and biofilms of MRSA, and cause leakage of proteins and nucleic acids. PG also could cause cells die due to the oxidative stress by inhibition of SOD and CAT activities. The differences of accumulation metabolites (DAMs) and expressed genes (DEGs) were mainly involved in ribosome, ABC transporters, DNA replication and repair, amino acid synthesis and metabolism, and carbohydrate metabolism according to the metabolomics and transcriptomics. In conclusion, the present study explored the bacteriostatic activity and inhibitory mechanism of PG, which will provide a research basis for PG’s development and application in food safety.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"154 ","pages":"Pages 143-156"},"PeriodicalIF":3.7,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143883137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0