Process Biochemistry最新文献_第3页

Development of a versatile and high-loading capacity yeast surface assembly system via SpyC/SpyT conjugation 利用SpyC/SpyT偶联技术开发一种多功能高负荷酵母表面组装系统

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-04-12 DOI: 10.1016/j.procbio.2025.04.004

Ke Xu , Hailin Liu , Chao Du , Faying Zhang , Wenya Wang , Guimin Zhang

{"title":"Development of a versatile and high-loading capacity yeast surface assembly system via SpyC/SpyT conjugation","authors":"Ke Xu , Hailin Liu , Chao Du , Faying Zhang , Wenya Wang , Guimin Zhang","doi":"10.1016/j.procbio.2025.04.004","DOIUrl":"10.1016/j.procbio.2025.04.004","url":null,"abstract":"<div><div>The yeast surface display (YSD) system for enzyme immobilization has a wide range of applications and is of great value in various studies. However, despite the many advantages of the YSD system, the amount of proteins it can display is limited, which restricts its potential applications. To enhance the applicability of the YSD system, we introduced a highly efficient and stable conjugation tool (SpyCatcher-SpyTag (SpyC-SpyT)) into the YSD system and developed a <em>Saccharomyces cerevisiae</em> surface assembly (SSA) system with high loading capacity. The SpyC-SpyT can spontaneously form molecular isopeptide bonds, and the target proteins can be assembled in the SSA system within 5 min. By increasing the copy of SpyC and integrating the expression fragment into the rDNA region, we successfully screened a genetically stable and high-copy strain that could assemble ∼7.8 × 10<sup>6</sup> SpyT-eGFP molecular count/cell. The organophosphate hydrolase immobilized by the SSA system not only has the similar enzyme activity as the free enzyme, but also has improved thermal stability and enhanced tolerance to acidic and alkaline environments. Further, we found that the immobilized enzyme still retained 77.7 % of its enzyme activity after 6 cycles. In conclusion, we successfully constructed a novel, efficient and stable SSA system for the development of the YSD system.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"155 ","pages":"Pages 13-22"},"PeriodicalIF":3.7,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143848702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Covalent immobilization of glucoamylase on 3D-printed poly(lactic acid) carriers for starch hydrolysis in stirred tank reactor 在 3D 打印聚（乳酸）载体上共价固定葡萄糖淀粉酶，用于搅拌罐反应器中的淀粉水解

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-04-11 DOI: 10.1016/j.procbio.2025.04.003

David Spressão de Lima Junior , Ilana Sessak , Daniela Remonatto , Julián Paul Martínez Galán , Murilo Daniel de Mello Innocentini , Ariela Veloso de Paula , Daniela Alonso Bocchini

{"title":"Covalent immobilization of glucoamylase on 3D-printed poly(lactic acid) carriers for starch hydrolysis in stirred tank reactor","authors":"David Spressão de Lima Junior , Ilana Sessak , Daniela Remonatto , Julián Paul Martínez Galán , Murilo Daniel de Mello Innocentini , Ariela Veloso de Paula , Daniela Alonso Bocchini","doi":"10.1016/j.procbio.2025.04.003","DOIUrl":"10.1016/j.procbio.2025.04.003","url":null,"abstract":"<div><div>This study presents a novel 3D-printed poly(lactic acid) (PLA) carrier for the covalent immobilization of a commercial glucoamylase. Enzyme carriers were functionalized by aminolysis with ethylenediamine (EDA) and activated with glutaraldehyde. Since covalent immobilization using glutaraldehyde as activating agent involves the formation of imine bonds which are inherently unstable, the use of borohydride as a reducing agent to stabilize these imine bonds after the functionalization step was considered. The highest enzymatic activity (13.68 U g<sup>−1</sup> carrier) was obtained when immobilization was performed at pH 10 using NaBH<sub>4</sub> (immobilization yield of 32.80 % ± 0.51 %). Optimal activity conditions were pH 4.92 and 50 °C for the soluble enzyme and pH 5.5 and 50 °C for the immobilized enzyme. The operational stability of immobilized glucoamylase was evaluated for 10 consecutive reaction cycles (of 10 minutes each) and the enzyme maintained 65.19 % of its original activity at the end of the third cycle. Starch saccharification in a stirred tank reactor by immobilized glucoamylase reached 95 % conversion after 12 h and 74 % conversion after 12 h in the first reuse cycle. These findings demonstrate the potential of PLA for glucoamylase immobilization, offering promising prospects for efficient and sustainable starch hydrolysis in industrial enzymatic processes.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"154 ","pages":"Pages 52-62"},"PeriodicalIF":3.7,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143834315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Medicinal plants are the basis of natural cosmetics 药用植物是天然化妆品的基础

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-04-10 DOI: 10.1016/j.procbio.2025.04.009

Olga Babich , Svetlana Ivanova , Alina Bakhtiyarova , Olga Kalashnikova , Stanislav Sukhikh

{"title":"Medicinal plants are the basis of natural cosmetics","authors":"Olga Babich , Svetlana Ivanova , Alina Bakhtiyarova , Olga Kalashnikova , Stanislav Sukhikh","doi":"10.1016/j.procbio.2025.04.009","DOIUrl":"10.1016/j.procbio.2025.04.009","url":null,"abstract":"<div><div>Plant-based cosmetics are made with a variety of cosmetic ingredients as the base, but they do not contain harmful synthetic chemical compounds that are toxic to humans. Plant-based cosmetics are safer and more natural than traditional cosmetics. The market for natural beauty products is constantly expanding, in parallel with consumer demand. These cosmetics have therapeutic properties ranging from anti-aging to hair care products. Plant-based cosmetics appeal to consumers due to their greater affordability and fewer side effects. This paper aims to analyze the plants that appeal to the cosmetic industry, as well as their primary applications. Currently, there is a lack of research reports on the antioxidant, anti-inflammatory, and antimicrobial potential of plants. In this study, plant bioactive compounds that showed significant bactericidal activity against antibiotic-resistant Gram-positive bacteria <em>in vitro</em> were investigated. Plant extracts act as adjuvants to antibiotics, enhancing the activity of several commonly used antibiotics against both Gram-positive and Gram-negative antibiotic-resistant bacteria without cytotoxicity to cells. In addition, biologically active compounds from plants show significant effects on wound treatment and healing <em>in vivo</em>. It is known that medicinal plants with biological antioxidant, anti-inflammatory, and antimicrobial components can be used in the treatment of skin infections and wound healing.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"154 ","pages":"Pages 35-51"},"PeriodicalIF":3.7,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143834316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An integrated study on the interactions of Lactobacillus brevis components with deoxynivalenol and zearalenone 短乳杆菌成分与脱氧雪腐菌醇和玉米赤霉烯酮相互作用的综合研究

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-04-10 DOI: 10.1016/j.procbio.2025.04.005

Fatemeh Adami Ghamsari , Abdolamir Ghadaksaz , Maryam Tajabadi Ebrahimi , Maryam Bagheri Varzaneh , Alireza Iranbakhsh , Abbas Akhavan Sepahi

{"title":"An integrated study on the interactions of Lactobacillus brevis components with deoxynivalenol and zearalenone","authors":"Fatemeh Adami Ghamsari , Abdolamir Ghadaksaz , Maryam Tajabadi Ebrahimi , Maryam Bagheri Varzaneh , Alireza Iranbakhsh , Abbas Akhavan Sepahi","doi":"10.1016/j.procbio.2025.04.005","DOIUrl":"10.1016/j.procbio.2025.04.005","url":null,"abstract":"<div><div>Deoxynivalenol (DON) and zearalenone (ZEN) are toxic <em>Fusarium</em> mycotoxins that frequently contaminate food and feed, posing significant public health risks. Among mitigation strategies, biological methods using lactic acid bacteria (LAB; e.g., <em>Lactobacillus</em> spp.) show particular promise, as these microorganisms can effectively biodegrade mycotoxins into less toxic or inactive metabolites. This study explored the binding and biotransformation capabilities of <em>Lactobacillus brevis</em> components (S-layer, aryl-alcohol dehydrogenase, carboxylesterase, and lipoteichoic acid repeats) using computational docking and Fourier transform infrared (FTIR) spectroscopy. Docking simulations revealed stronger binding affinities for ZEN than DON, involving interactions with hydroxyl groups, oxygen atoms, alkyl chains, and aromatic rings. Enzymes formed stable complexes with mycotoxins, suggesting biotransformation potential. FTIR spectra after 24 hours of incubation revealed mycotoxin adsorption through interactions with bacterial cell wall components, indicated by C-H and C-C peak shifts. For DON, changes in OH and C<img>O peaks suggested oxidation-reduction, likely mediated by aryl-alcohol dehydrogenase, producing 3-keto-DON and 3-epi-DON. For ZEN, shifts in OH, C-O, and C<img>O peaks indicated lactone ring hydrolysis, likely catalyzed by carboxylesterase. These findings highlight <em>L. brevis</em> as a promising biocontrol agent that detoxifies DON and ZEN through binding and biotransformation, offering a potential strategy to mitigate mycotoxin contamination in food and feed.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"154 ","pages":"Pages 12-21"},"PeriodicalIF":3.7,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Affinity resin selection for efficient capture of bispecific antibodies as guided by domain composition 根据结构域组成选择亲和树脂，高效捕获双特异性抗体

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-04-08 DOI: 10.1016/j.procbio.2025.04.007

Nattha Ingavat , Xinhui Wang , Yee Jiun Kok , Nuruljannah Dzulkiflie , Han Ping Loh , Eunice Leong , Kia Ngee Low , Amihan Anajao , Say Kong Ng , Yuansheng Yang , Xuezhi Bi , Wei Zhang

{"title":"Affinity resin selection for efficient capture of bispecific antibodies as guided by domain composition","authors":"Nattha Ingavat , Xinhui Wang , Yee Jiun Kok , Nuruljannah Dzulkiflie , Han Ping Loh , Eunice Leong , Kia Ngee Low , Amihan Anajao , Say Kong Ng , Yuansheng Yang , Xuezhi Bi , Wei Zhang","doi":"10.1016/j.procbio.2025.04.007","DOIUrl":"10.1016/j.procbio.2025.04.007","url":null,"abstract":"<div><div>Affinity chromatography is a cornerstone of bispecific antibody (bsAb) purification, with resin selection playing a critical role in developing downstream processes to ensure both process efficiency and product quality. Unlike monoclonal antibody purification, where Protein A chromatography is the gold standard for antibody capture, affinity chromatography in bsAb purification is often employed not only for capture but also for the removal of hard-to-eliminate product-related impurities. This study demonstrates that affinity resin selection can be effectively guided by analysing the domain composition of the target bsAb molecule and its potential impurities. Using faricimab, a CH1-CL CrossMab, as a model, Protein L — an affinity resin targeting the variable region of the light chain — was predicted to be the most effective affinity chromatography due to its different binding avidity towards faricimab from its major product-related impurities. Validation through screening four different types of affinity chromatography, each binding to distinct regions of faricimab, confirmed this prediction. Under optimized elution conditions, the purification process achieved ∼73 % purity from ∼30 % in the culture with ∼86 % monomeric yield, as well as decent removal of host cell proteins and host cell DNA.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"154 ","pages":"Pages 1-11"},"PeriodicalIF":3.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143821411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient removal of atrazine in wastewater by washed peanut shells biochar: Adsorption behavior and biodegradation 花生壳生物炭对废水中阿特拉津的高效去除：吸附行为和生物降解

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-04-08 DOI: 10.1016/j.procbio.2025.04.008

Yidan Wang , Yuhang Chen , Lianying Wang , Yayuan Mo , Xiangfeng Lin , Shu Gao , Menglin Chen

{"title":"Efficient removal of atrazine in wastewater by washed peanut shells biochar: Adsorption behavior and biodegradation","authors":"Yidan Wang , Yuhang Chen , Lianying Wang , Yayuan Mo , Xiangfeng Lin , Shu Gao , Menglin Chen","doi":"10.1016/j.procbio.2025.04.008","DOIUrl":"10.1016/j.procbio.2025.04.008","url":null,"abstract":"<div><div>Peanut shell powder was washed with water and then calcined to produce biochar, which acted as an adsorbent and biofilm supporter for the removal of atrazine (ATR). Biochar sample prepared by washing 6 h (named BC-W) achieved the highest biodegradation amount (<em>Q</em><sub><em>m</em></sub><em>,</em> 12.48 mg/g). The adsorption process of ATR on BC-W involved hydrogen bonding and π-π interactions. We found that the removal rate of ATR by biodegradation was 6.34–6.75 times that of adsorption, with an ATR concentration of 30 mg/L under half aeration. The results of high-throughput sequencing showed that there were two dominant bacterial phyla in the BC-W samples after biofilm culture: <em>Firmicutes</em> (51.89 %) and <em>Proteobacteria</em> (47.79 %), among which <em>Bacillus</em>, <em>Clostridium</em> (<em>sensu stricto</em>), and <em>Pseudomonas</em>, played a major role in the biodegradation of ATR. These findings showed that biochar could be employed as a low-cost adsorbent for the removal of pesticides from wastewater, also a biofilm supporter for enhanced biodegradation of organic pollutants.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"154 ","pages":"Pages 22-34"},"PeriodicalIF":3.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143825807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Valorization of Solanum melongena L. crop by-products: Phenolic composition and in vitro antioxidant, antidiabetic, anti-inflammatory, cytotoxic, and antimicrobial properties 茄类作物副产品的增值：酚类成分及其体外抗氧化、抗糖尿病、抗炎、细胞毒性和抗菌特性

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-04-07 DOI: 10.1016/j.procbio.2025.04.002

Mikel Añibarro-Ortega , Maria Inês Dias , Jovana Petrović , Sonia Núñez , Ricardo C. Calhelha , Eduardo M. Costa , M. Machado , Manuela Pintado , Marina Soković , Víctor López , Lillian Barros , José Pinela

{"title":"Valorization of Solanum melongena L. crop by-products: Phenolic composition and in vitro antioxidant, antidiabetic, anti-inflammatory, cytotoxic, and antimicrobial properties","authors":"Mikel Añibarro-Ortega , Maria Inês Dias , Jovana Petrović , Sonia Núñez , Ricardo C. Calhelha , Eduardo M. Costa , M. Machado , Manuela Pintado , Marina Soković , Víctor López , Lillian Barros , José Pinela","doi":"10.1016/j.procbio.2025.04.002","DOIUrl":"10.1016/j.procbio.2025.04.002","url":null,"abstract":"<div><div>This study explored the valorization of post-harvest eggplant aerial parts as a sustainable source of value-added ingredients by investigating their phenolic composition and <em>in vitro</em> bioactive properties. HPLC-DAD-ESI/MS<sup><em>n</em></sup> analysis identified chlorogenic acid derivatives as the predominant phenolic compounds (53 % of the phenolic fraction), followed by <em>O</em>-glycosylated kaempferol and quercetin. The extract displayed antioxidant activity in physiologically relevant cell-based assays and significant α-glucosidase inhibitory capacity that far exceeded that of the standard drug acarbose. It also inhibited the formation of advanced glycation end-products (AGEs), suggesting its potential to mitigate diabetes-related complications. Furthermore, the extract showed a modest pancreatic lipase inhibitory effect and capacity to suppress interleukin 6 production. Selective cytotoxicity against human gastric and colon adenocarcinoma cell lines and strong antimicrobial activity against foodborne pathogens were observed. Given the growing demand for natural alternatives to synthetic drugs, these findings position eggplant crop biomass as a promising, sustainable source of active compounds with potential applications in food, nutraceutical, and pharmaceutical formulations for managing type 2 diabetes and other oxidative stress-mediated conditions. This study not only contributes to the valorization of agricultural waste but also expands the research on by-products of Solanaceae crops, offering a pathway for sustainable resource utilization.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"153 ","pages":"Pages 315-324"},"PeriodicalIF":3.7,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143816455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of lactic acid bacteria fermentation on biotransformation of phenolic compounds and bioactivities of hawthorn pulp based on metabolomics 基于代谢组学的乳酸菌发酵对山楂果肉酚类化合物生物转化及生物活性的影响

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-04-04 DOI: 10.1016/j.procbio.2025.03.021

Wanting Zhang, Xiaomin Yang, Yan Xu, Bingjie Liu, Xianghong Meng

{"title":"Effect of lactic acid bacteria fermentation on biotransformation of phenolic compounds and bioactivities of hawthorn pulp based on metabolomics","authors":"Wanting Zhang, Xiaomin Yang, Yan Xu, Bingjie Liu, Xianghong Meng","doi":"10.1016/j.procbio.2025.03.021","DOIUrl":"10.1016/j.procbio.2025.03.021","url":null,"abstract":"<div><div>Lactic acid bacteria (LAB) fermentation can offer an improvement in the nutritional quality and biological activity of foods. This study aimed to explain the impacts of LAB fermentation on bioactivities and biotransformation of polyphenols in hawthorn pulp. Firstly, a strain with better polyphenol transformation capacity was selected from four LAB strains; then, microbial characteristics, polyphenols content, antioxidant capacity, and digestive enzymes inhibitory capacity of fermented hawthorn pulp were evaluated and their correlation was established; finally, metabolomics was used for analyzing the biotransformation of polyphenols. The results showed that <em>Lactobacillus rhamnosus</em> FN518 exhibited the best efficiency, which reached viable counts of 8.28 ± 0.03 log CFU/mL after 48 h of fermentation and exhibited strong malolactic-conversion ability. The total phenolic content significantly increased to 11.25 ± 0.12 mg GAE/g and DPPH radical scavenging capacity, FRAP, and α-amylase inhibitory capacity significantly increased by 30.80 %, 13.21 %, and 16.82 %, respectively (<em>p</em> < 0.05), which were significantly and positively correlated with phenolic compounds. Metabolomics revealed that flavonoid biosynthesis and enzymatic degradation of macromolecular phenolic compounds were primary biotransformation pathways of polyphenols in fermented hawthorn pulp. This study contributes precious insights into biotransformation of hawthorn polyphenols via LAB fermentation and development of fermented hawthorn products.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"153 ","pages":"Pages 304-314"},"PeriodicalIF":3.7,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143808155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A mechanistic insight into polyethylene degradation by Bacillus sp. and, Lysinibacillus sp. from mangrove soil 芽孢杆菌和赖氨酸芽孢杆菌从红树林土壤中降解聚乙烯的机理研究

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-04-04 DOI: 10.1016/j.procbio.2025.03.018

N.D. Dhanraj , U.P. Sreelakshmi , P. Sneha , M.S. Jisha

{"title":"A mechanistic insight into polyethylene degradation by Bacillus sp. and, Lysinibacillus sp. from mangrove soil","authors":"N.D. Dhanraj , U.P. Sreelakshmi , P. Sneha , M.S. Jisha","doi":"10.1016/j.procbio.2025.03.018","DOIUrl":"10.1016/j.procbio.2025.03.018","url":null,"abstract":"<div><div>This study aims to establish a sustainable method for plastic remediation, particularly focusing on polyethylene (PE). Bacteria capable of breaking down PE were identified and characterized from plastic-contaminated mangrove soils in Mangalavanam and Puthuvype, where limited efforts had been made to explore PE-degrading microbes. Two isolates, Ma21 and Pu9, showed efficient PE degradation, which exhibited 7.8 % and 4.2 % weight loss of PE film respectively. This degradation was supported by FTIR analysis, indicating initial degradation steps, and FE-SEM analysis, showing surface deterioration of PE films due to bacterial action. GC-MS analysis of reaction intermediates detected phthalic acid compounds, suggesting the ability of these bacteria to degrade PE and indicating the presence of plastic additives in the degradation medium. Screening for biosurfactant production revealed that both Ma21 and Pu9 can produce biosurfactants. The production of alkane hydroxylase enzyme and laccase enzyme by Ma21 and Pu9 further supported their biodegradation capabilities. Molecular identification through 16S rDNA sequencing confirmed that Ma21 is <em>Bacillus thuringiensis</em>, and Pu9 is <em>Lysinibacillus boronitolerans</em>.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"153 ","pages":"Pages 294-303"},"PeriodicalIF":3.7,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143808156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced 2-phenylethanol and co-production of pullulan from synthetic medium by Aureobasidium pullulans 普鲁兰小孢子菌合成培养基增强2-苯乙醇及协同生产普鲁兰

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-04-04 DOI: 10.1016/j.procbio.2025.03.025

Lijun Wu , Guoqiang Chen , Xinruitong Liu , Yuxi Wei , Lijuan Liu , Weiyao Hu , Haibo Zhang

{"title":"Enhanced 2-phenylethanol and co-production of pullulan from synthetic medium by Aureobasidium pullulans","authors":"Lijun Wu , Guoqiang Chen , Xinruitong Liu , Yuxi Wei , Lijuan Liu , Weiyao Hu , Haibo Zhang","doi":"10.1016/j.procbio.2025.03.025","DOIUrl":"10.1016/j.procbio.2025.03.025","url":null,"abstract":"<div><div>2-phenylethanol (2-PE) and its derivatives play crucial roles in food flavoring and fine chemical synthesis. However, their industrial-scale production yields remain a key constraint for broader industrial implementation. To overcome this challenge, we implemented an integrated <em>in-situ</em> product adsorption (ISPR) strategy combined with nitrogen source restriction and a high-glucose medium formulation during 2-PE biosynthesis using <em>Aureobasidium pullulans</em> (<em>A</em>. <em>pullulans</em>) A5. Notably, this approach enabled simultaneous co-production of 2-PE and the commercially valuable exopolysaccharide pullulan. The experimental protocol comprised two phases: First, we established a basal production medium (medium-1) containing 20 % (w/v) glucose and 1.8 % (w/v) Yeast Nitrogen Base (YNB). Subsequently, we systematically screened adsorption agents, identifying Trialkylphosphine Oxide (TRPO) as an optimal extractant that demonstrated selective 2-PE adsorption capacity but not for L-phenylalanine (L-phe). In the optimized system, supplementation of 10 mL TRPO to 50 mL medium-1 (pH 6.0) supplemented with 4 g/L of L-phe as precursor resulted in 2.39 g/L 2-PE production (60 % molar conversion efficiency) and 5.16 g/L pullulan accumulation after 48 h fermentation. Process analysis revealed that TRPO-mediated ISPR enhanced 2-PE volumetric productivity, while the high-carbon medium configuration synergistically increased both target metabolite titers. These technical improvements significantly improved process economics while simultaneously streamlining downstream processing for industrial-scale operations</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"153 ","pages":"Pages 259-266"},"PeriodicalIF":3.7,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143776735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0