Process Biochemistry最新文献

{"title":"Phosphorus and sulphur affecting the growth, nutrient uptake and amino acids composition in Dunaliella salina","authors":"Yixing Sui ,&nbsp;Gleison de Souza Celente ,&nbsp;Laurens Bosscher Navarro ,&nbsp;Pablo Garcia-Trinanes ,&nbsp;Patricia J. Harvey","doi":"10.1016/j.procbio.2025.03.026","DOIUrl":"10.1016/j.procbio.2025.03.026","url":null,"abstract":"<div><div>The potential of microalga <em>Dunaliella salina</em> as a protein source and in bioremediation application is still underdeveloped, despite its known carotenoids overproduction. This study focused on investigating the effects of phosphorus (P) and sulphur (S) concentrations on the biomass growth and essential amino acids profile of <em>D. salina</em> PLY DF15 (CCAP 19/41). Additionally, its nutrient removal capacity was analysed in a bioremediation context. From all tested P and S concentrations, <em>D. salina</em> resulted in high-quality protein with essential amino acid index (EAAI) between 1.6 and 1.7 (EAAI ≥ 1 meets FAO/WHO standard) with little variations from all P and S concentrations. In a bioremediation context, <em>D. salina</em> also showed great potential for nutrients removal and recovery with high adaptability to varying nutrient conditions, with overall more than 80 % and 100 % nitrogen (N) and P recovery, respectively. The full recovery of S however was only achieved at lower S levels. Overall <em>D. salina</em> shows great potential in providing high-quality protein for human consumption using economic and sustainable cultivation approach.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"153 ","pages":"Pages 276-283"},"PeriodicalIF":3.7,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143791203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"One-pot chemo-enzymatic cascades using ene-reductase and alcohol dehydrogenase to produce a brivaracetam chiral precursor","authors":"Jiacheng Feng ,&nbsp;Dingjun Chu ,&nbsp;Ruiwei Chen ,&nbsp;Xiaoqiang Xie ,&nbsp;Yongxian Fan ,&nbsp;Xiaolong Chen ,&nbsp;Linjiang Zhu","doi":"10.1016/j.procbio.2025.04.001","DOIUrl":"10.1016/j.procbio.2025.04.001","url":null,"abstract":"<div><div>Brivaracetam is a third-generation antiepileptic drug containing two chiral centers (<em>2S</em>, <em>4 R</em>). To achieve effective asymmetric synthesis of 4 <em>R</em> chiral center, a novel one-pot chemo-enzymatic cascade strategy was developed to synthesize the 4 <em>R</em> chiral precursor, (<em>R</em>)-4-propyldihydrofuran-2(3<em>H</em>)-one (<strong>1b</strong>). The butenolide 5-hydroxy-4-propylfuran-2(5<em>H</em>)-one was selected as the starting substrate due to the presence of an active electron-withdrawing group, which enabled rapid enzymatic reduction by an ene-reductase with &gt; 99 % conversion. However,‌ this reaction generated ‌a racemization-prone intermediate‌. Subsequent reduction ‌using a newly identified alcohol dehydrogenase (YahK)‌ demonstrated high activity but ‌exhibited <em>S</em>-configuration stereoselectivity‌. ‌ Through semi-rational engineering, the stereoselectivity of a YahK triple mutant (G132T/T182A/M313R) was successfully reversed to favor the <em>R</em>-configuration. The chemo-enzymatic cascade combining ADH reduction and chemical cyclization yielded the chiral product <em>4R</em>-<strong>1b</strong> with an enantiomeric excess (<em>ee</em>) of &gt; 91 % (<em>R</em>). Notably, the one-pot mode further enhanced the <em>ee</em> value of the final product to &gt; 98 %. This strategy effectively bypassed the limitations of low-reactivity alkene substrates, enabling efficient construction of the <em>4 R</em> chiral center in brivaracetam.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"153 ","pages":"Pages 267-275"},"PeriodicalIF":3.7,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143785098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shedding light on H. pylori detection: A fusion protein approach unveiled through LIPS method","authors":"Seyedeh Mahsa Farzanfar ,&nbsp;Sedigheh Asad","doi":"10.1016/j.procbio.2025.03.023","DOIUrl":"10.1016/j.procbio.2025.03.023","url":null,"abstract":"<div><div>The Luciferase Immunoprecipitation Systems (LIPS) method is a highly sensitive approach for quantitatively detecting antibodies, offering potential in identifying viral and bacterial infections. However, the substantial size of the luciferase-antigen fusion protein presents challenges in production and folding. Using epitopes rather than the full-length antigenic protein may circumvent issues with recombinant expression. <em>Helicobacter pylori</em>, a gram-negative bacterium, poses a risk of gastric cancer if untreated. This study focuses on producing a fusion protein comprising in silico-designed antigenic epitopes from the <em>H. pylori</em> urease protein and luciferase, aiming to reduce the fusion protein's size and augment its expression in the <em>E. coli</em> system. Bioinformatic analysis identified sequences encoding antigenic regions, which were amplified via PCR. A luciferase-linker-epitope construct was then devised and expressed in the <em>E. coli Bl21</em> (DE3) strain. The recombinant protein was primarily purified to homogeneity, yielding a major band at 75 kilodaltons. Verification of the protein's proper folding and functionality was confirmed through a bioluminescence assay with an emission of 13.7 × 10^6 RLU/s. Western blot analysis authenticated the fusion protein's specific binding to <em>H. pylori</em> antibodies. These findings underscore the potential of the protein as a promising candidate for <em>H. pylori</em> detection and streamline LIPS fusion protein production.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"153 ","pages":"Pages 212-219"},"PeriodicalIF":3.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impacts of in vitro gastrointestinal digestion on phenolic compounds bioaccessibility and biological activities of uvaia (Eugenia pyriformis Cambess) fractions","authors":"David de Paulo Farias ,&nbsp;Fábio Fernandes de Araújo ,&nbsp;Alexandra CHF Sawaya ,&nbsp;Iramaia Angélica Neri-Numa ,&nbsp;Glaucia Maria Pastore","doi":"10.1016/j.procbio.2025.03.024","DOIUrl":"10.1016/j.procbio.2025.03.024","url":null,"abstract":"<div><div><em>Uvaia</em> (<em>Eugenia pyriformis</em> Cambess) is a species native to the Atlantic Rainforest (Brazil) that has a great potential for technological applications, but which remains unexplored in relation to its biological potential. Hereby, we evaluated the effect of <em>in vitro</em> gastrointestinal digestion on phenolic compounds bioaccessibility, antioxidant capacity, α-amylase, α-glucosidase inhibition and antiglycation activity of two <em>uvaia</em> portions: edible fraction and seed. The phenolic characterization, antioxidant capacity and antidiabetic potential of the aqueous phenolic extract of both <em>uvaia</em> fractions were also evaluated. UHPLC-MS/MS analysis in the aqueous extract showed that quinic acid was the main organic acid present in <em>uvaia</em> seed (171.91 mg/100 g), whereas quercitrin was found mainly in the edible fraction (16.00 mg/100 g). <em>Uvaia</em> seed had the greatest antioxidant capacity (9951 – 37500 µmol Trolox equivalent (TE)/100 g) compared with edible fraction (1900 – 9123 µmol TE/100 g). Edible fraction showed higher <em>α</em>-amylase inhibitory potential (IC50 = 5.72 mg/mL), while there was no difference between both evaluated fractions for <em>α</em>- glucosidase inhibition (IC50 = 5.24–5.26 mg/mL). <em>Uvaia</em> seed showed higher antiglycation potential (IC50 = 1.09 mg/mL) compared with the edible fraction (IC50 = 8.46 mg/mL). Regarding the phenolics bioaccessibility, rutin and epicatechin significantly decreased after gastrointestinal digestion for both fractions. However, <em>p</em>-coumaric acid and quercitrin significantly increased after digestion for edible fraction (7 and 3-fold, respectively). In general, the antidiabetic potential of both <em>uvaia</em> fractions increased after gastrointestinal digestion. These results could encourage the development of new formulations with functional claims using different parts of <em>uvaia</em>.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"153 ","pages":"Pages 248-258"},"PeriodicalIF":3.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143776737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of operating parameters for pyrite-based electrochemical bioreactor using response surface methodology: C/N ratio, current density, and hydraulic retention time","authors":"Yufan Xie ,&nbsp;Sicheng Yuan ,&nbsp;Guangjun Wang ,&nbsp;Qinglong Wang ,&nbsp;Yuhu Nie ,&nbsp;Weibo Wang ,&nbsp;Shiyang Zhang","doi":"10.1016/j.procbio.2025.03.020","DOIUrl":"10.1016/j.procbio.2025.03.020","url":null,"abstract":"<div><div>This study optimized three important influence factors of C/N ratio, current density (CD), and hydraulic retention time (HRT) using Box-Behnken design and response surface methodology (RSM) for a pyrite-based electrochemical bioreactor (PEBR). Meanwhile, the interactive effects among these factors were investigated. Under the optimal conditions, the NO₃^--N removal efficiency (NRE) peaked at 95.61 % at an HRT of 12 h, a CD of 483 mA/m², and a C/N ratio of 3.55. Among these factors, the C/N ratio was identified as the most influential factor affecting NRE. Under the optimal conditions, Proteobacteria (43.36 %), Campylobacterota (11.14 %), Bacteroidota (8.00 %), and Desulfobacterota (11.36 %) were the dominant phyla, while <em>Sulfurimonas</em> (7.19 %), <em>Acetobacterium</em> (7.11 %) and <em>Desulfovibrio</em> (7.11 %) became the dominant genera. In addition, the relative abundance of functional genes for sulfur metabolism was enhanced obviously after the operating parameter optimization. These findings can help PEBR achieve high efficiency and low energy consumption for nitrate removal.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"153 ","pages":"Pages 191-197"},"PeriodicalIF":3.7,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143746586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AChE inhibition sensing through SiO₂ and GQDs interface on screen-printed electrode for malathion detection","authors":"Jyoti Yadav ,&nbsp;Vinita Hooda ,&nbsp;Nidhi Chauhan","doi":"10.1016/j.procbio.2025.03.019","DOIUrl":"10.1016/j.procbio.2025.03.019","url":null,"abstract":"<div><div>Organophosphorus pesticides like malathion, adversely affect and cause significant risks to human health and the environment. Conventional detection methods are often pricey and tedious and need specialists. In this study, an electrochemical biosensor for malathion detection was developed using acetylcholinesterase (AChE) inhibition. The sensor was created by modifying a screen-printed electrode with silica nanoparticles (SiO₂) and graphene quantum dots (GQDs), followed by the immobilization of AChE. Square wave voltammetry was used to evaluate of the sensor performance using acetylthiocholine chloride (ATCl) with a detection range of 40–100 pM with a significant current difference of 9 µA. The biosensor demonstrated a sensitivity of 0.10 µA/pM for the substrate ATCl and achieved a detection limit of 0.01 ppm for malathion. It effectively detected malathion in various vegetable samples, highlighting its potential for on-site pesticide monitoring and rapid detection.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"153 ","pages":"Pages 238-247"},"PeriodicalIF":3.7,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143776736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic engineering of Mycobacterium fortuitum for high-level production of sitolactone from biorenewable phytosterols","authors":"Xinghui Zhai ,&nbsp;Chenyang Yuan ,&nbsp;Xiaoxuan Sun ,&nbsp;Wen Sun ,&nbsp;Beiru He ,&nbsp;Ruijie Zhang ,&nbsp;Baoguo Zhang","doi":"10.1016/j.procbio.2025.03.022","DOIUrl":"10.1016/j.procbio.2025.03.022","url":null,"abstract":"<div><div>Recent advances in steroid drug synthesis have increasingly focused on leveraging biosynthetic intermediates derived from phytosterols, an economical and abundant raw material for microbial metabolism. Sitolactone (3aα-H-4α-(3’-Propionic acid)-5α-hydroxy-7aβ-methylhexahydro-1-indanone-Δ-lactone, or HIL) serves as a key precursor for the synthesis of steroid drugs, particularly those either devoid of a methyl group or featuring an α-methyl group instead of a β-methyl group at the C-10 position. In preliminary investigations, we achieved HIL production with a purity of only 63.9 % through the inactivation of the CAR1, CAR2, and FadE30 genes in <em>Mycobacterium fortuitum</em> ATCC 6842. To address the accumulation of by-products, we subsequently identified and inactivated three isoenzymes of FadE30 (FadE31, FadE32, FadE33), leading to an HIL purity exceeded 99 %. The optimized strain demonstrated the ability to convert 10 g/L of phytosterols into 4.84 g/L of HIL within 72 hours, achieving a molar yield of 90.2 %—the highest reported yield to date. Bioconversion experiments using phytosterol concentrations at 50 g/L yielded a maximum space-time productivity of 3.66 g/L/d with an 81.87 % molar yield, marks the highest yield recorded. These findings provide a cost-effective and efficient method for producing 19-norsteroid intermediates in pharmaceutical manufacturing while contributing valuable insights into steroid nucleus metabolism.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"153 ","pages":"Pages 230-237"},"PeriodicalIF":3.7,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fermentation of dehydrated fruit juices by Lactiplantibacillus plantarum with prebiotic supplementation as non-dairy functional beverages","authors":"Nurhazwani Sa’aid,&nbsp;Joo Shun Tan","doi":"10.1016/j.procbio.2025.03.015","DOIUrl":"10.1016/j.procbio.2025.03.015","url":null,"abstract":"<div><div>This study elucidates the impact of prebiotics, specifically inulin and galacto-oligosaccharides (GOS), on the fermentation of dehydrated fruit juices. <em>Lactiplantibacillus plantarum</em>, a probiotic strain, was employed as the fermentative agent, with growth dynamics monitored over 72 hours across various fruit juice substrates; mango, pineapple, dragon fruit, cranberry and mixed berry juices. Results revealed that inulin supplementation notably enhanced <em>L. plantarum</em> proliferation, surpassing GOS and lactose controls across diverse fruit juice matrices, with the highest growth observed at 12 hours in mango juice (1.32 ± 0.014) and 24 hours in pineapple juice (0.68 ± 0.003). The prebiotic index for inulin-supplemented juices was consistently above one, indicating positive probiotic growth. Lactic acid production was also higher in inulin-supplemented juices, with mango juice reaching 10.22 ± 0.004 g/L compared to 8.66 ± 0.007 g/L in the control. Further investigations encompassed free radical scavenging activity, carbohydrate-hydrolysing enzymes and lipid digestion enzyme inhibition, unveiling nuanced effects influenced by prebiotics and fermentation. Notably, a comprehensive heat-map analysis provided insights into the metabolic alterations induced by fermentation and prebiotic supplementation. These findings suggest that fermented fruit juices supplemented with inulin have the potential to offer enhanced nutritional value and health benefits.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"153 ","pages":"Pages 220-229"},"PeriodicalIF":3.7,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of the production of a lipase from Staphylococcus pasteuri and its immobilization/stabilization on a hydrophobic support","authors":"Amina Tiouche ,&nbsp;Hayate Haddadi-Guemghar ,&nbsp;Naima Guendouz ,&nbsp;Atika Meklat ,&nbsp;El Hocine Siar ,&nbsp;Tahar Benayad ,&nbsp;Amina Kamah ,&nbsp;Samir Hadjal ,&nbsp;Lila Boulakbeche ,&nbsp;Roberto Fernandez-Lafuente","doi":"10.1016/j.procbio.2025.03.017","DOIUrl":"10.1016/j.procbio.2025.03.017","url":null,"abstract":"<div><div>The production of an extracellular lipase from the <em>Staphylococcus pasteuri</em> 3''- 4₂ strain was optimized using a design of experiments methodology. Firstly, the Plackett-Burman Design revealed that olive oil, meat extract, and ammonium sulfate had the strongest positive influence on lipase production among 15 tested variables. Next, a three-level Central Composite Design resulted in a validated model with a p-value &lt; 0.001 and an R² of 0.983. Under optimal conditions—1.32 % (v/v) olive oil, 0.92 % (w/v) meat extract, 0.5 % (w/v) ammonium sulfate, and a 5.08 % (v/v) inoculum size—the lipase activity increased threefold, reaching 31.26 U/mL after 16 hours. The semi-purified lipase was immobilized by adsorption on a hydrophobic support via interfacial activation. Initially, the immobilization yield was quite poor, and this was explained by the formation of lipase aggregates. However, 100 % of the lipase activity was immobilized in 30 minutes when adding 3 M guanidine, with 95 % recovery of activity. The immobilized lipase showed higher activity and stability in the presence of organic solvents than the free enzyme.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"153 ","pages":"Pages 198-211"},"PeriodicalIF":3.7,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and development of pilot photobioreactor for simultaneous microalgae cultivation and aquaculture wastewater treatment","authors":"X. Christodoulou ,&nbsp;C. M'ahmed ,&nbsp;F. Zili ,&nbsp;B. Bessadok ,&nbsp;S. Sadok ,&nbsp;I. Monney ,&nbsp;R. Rothlisberger ,&nbsp;M. Bagnoud","doi":"10.1016/j.procbio.2025.03.016","DOIUrl":"10.1016/j.procbio.2025.03.016","url":null,"abstract":"<div><div>This study presents an innovative and modular phototrophic biofilm photobioreactor (PBR) designed for the simultaneous cultivation of algae and the treatment of aquaculture wastewater (AWW). The vertical flat-plate BPR allows for stable microalgae growth while efficiently removing nutrients from wastewater under controlled conditions, including light, CO₂, supplementation, water recirculation and continuous monitoring of parameters such as pH, nitrate (NO₃^-N) and phosphate (PO₄^3-P). The PBR was operated at an aquaculture facility using AWW, with nutrient removal and microalgal growth being monitored. The microalgae consortium composed of <em>Chlorella sp.</em>, <em>Scenedesmus sp.</em> and <em>Phormidium sp.</em> were evaluated for their growth potential and wastewater remediation capabilities. Results showed high nutrient removal efficiencies with 92 % reduction of PO₄^3-P (removal rate: 0.07 mg/L d) and a 62 % reduction of NO₃^--N (removal rate: 1.1 mg/L d), bringing nutrient concentrations below the limits set by the Waters Protection Ordinance. Maximum biomass production reached a growth rate on land surface of 25 g/m²/d, with a favorable biochemical composition of 51 % proteins, 25 % carbohydrates and up to 8 % lipids, indicating the potential for use animal feed. This study demonstrates the feasibility of using AWW as a growth medium for microalgae while simultaneously achieving wastewater remediation, offering a sustainable solution for nutrient recycling in aquaculture operations.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"153 ","pages":"Pages 182-190"},"PeriodicalIF":3.7,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143746585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}