Process Biochemistry最新文献_第4页

Unlocking the potential of CMP synthesis: Merging enzyme design, host genome editing, and fermentation optimization strategy

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-02-01 DOI: 10.1016/j.procbio.2024.12.017

Zhou-Lei Qing , Chuan-Qi Sun , Zong-Lin Li , Zhi-Min Li

{"title":"Unlocking the potential of CMP synthesis: Merging enzyme design, host genome editing, and fermentation optimization strategy","authors":"Zhou-Lei Qing , Chuan-Qi Sun , Zong-Lin Li , Zhi-Min Li","doi":"10.1016/j.procbio.2024.12.017","DOIUrl":"10.1016/j.procbio.2024.12.017","url":null,"abstract":"<div><div>Cytidine-5′-monophosphate (CMP) and its derivatives play an important role in the fields of food, medicine and cosmetics. In this study, we used crude enzyme preparations of nucleoside kinase (NK) and polyphosphate kinase (PPK) to synthesize CMP in a synergistic reaction. A variant NK enzyme A220L/A236V/V237A/H252A was developed through a rational channel engineering approach, and its activity was increased by 9.17 times compared with the wild type. However, when cytidine was used as a substrate, the accumulation of a large amount of byproducts hindered the reaction efficiency, resulting in CMP yields that were always less than 80 %. By knocking out the <em>cdd</em>, <em>rihA</em> and <em>rihC</em> genes related to cytidine metabolism in the <em>Escherichia coli</em> host genome, the CMP yield was significantly increased to more than 95 %. Subsequently, we optimized the fermentation process in a 5-L bioreactor to further improve the crude enzyme activity. The optimized crude enzyme coupling reaction successfully generated 291 mM CMP within 20 h with a yield of up to 97 %. This study significantly improved the production efficiency of CMP by integrating key enzyme engineering, host bacteria modification and fermentation process optimization, laying a solid foundation for its industrial-scale production.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"149 ","pages":"Pages 204-212"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143139248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro enzymatic production of 3-hydroxypropionic acid from glycerol via CoA-independent pathway

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-02-01 DOI: 10.1016/j.procbio.2024.12.023

Hanjoo Jo , Byeongsu Gu , Minkyeong Jeon , Sung In Lim

{"title":"In vitro enzymatic production of 3-hydroxypropionic acid from glycerol via CoA-independent pathway","authors":"Hanjoo Jo , Byeongsu Gu , Minkyeong Jeon , Sung In Lim","doi":"10.1016/j.procbio.2024.12.023","DOIUrl":"10.1016/j.procbio.2024.12.023","url":null,"abstract":"<div><div>3-Hydroxypropionic acid (3-HP) is a C3-based versatile platform chemical offering significant industrial and environmental advantages over petrochemical derivatives. Microbial cell factories are commonly employed to produce 3-HP from various carbon sources, with glycerol being a key substrate via CoA-dependent and -independent pathways. However, challenges such as inhibited growth and reduced productivity, often due to substrate inhibition and toxic byproducts like 3-hydroxypropionaldehyde (3-HPA), limit the efficiency of glycerol-based microbial 3-HP production and its industrial scalability. To address these challenges, we propose an alternative <em>in vitro</em> approach for 3-HP production from glycerol through a coupled enzymatic reaction. We optimized the recombinant production of glycerol dehydratase (GDHt) and alpha-ketoglutaric semialdehyde dehydrogenase (KGSADH), two key enzymes from <em>Escherichia coli</em>. After purification, GDHt and KGSADH showed 23.4 ± 4.6 s<sup>−1</sup> mM<sup>−1</sup> and 28.7 ± 5.0 s<sup>−1</sup> mM<sup>−1</sup> of k<sub>cat</sub>/K<sub>m</sub>, respectively. We then systematically varied enzyme concentrations, reaction temperature, and pH to determine the optimal conditions for 3-HP biosynthesis. The chain reaction, conducted with 100 nM of each enzyme, produced 43.5 μg/mL of 3-HP within 20 min under optimal conditions of 45 ºC and pH 9.0. This approach demonstrated the potential for a clean and efficient <em>in vitro</em> system for 3-HP production from renewable sources.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"149 ","pages":"Pages 288-294"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143139493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel myco-fabrication of copper and nickel nanoparticles and evaluation of their effects against antibiotic resistance genes in different bacterial strains and anticancer potentials

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-02-01 DOI: 10.1016/j.procbio.2024.12.008

Aisha Shaban , Zainab Zakaria , Ismail A. Abdelhamid , Heba K.A. Elhakim , El-Sayed R. El-Sayed

{"title":"Novel myco-fabrication of copper and nickel nanoparticles and evaluation of their effects against antibiotic resistance genes in different bacterial strains and anticancer potentials","authors":"Aisha Shaban , Zainab Zakaria , Ismail A. Abdelhamid , Heba K.A. Elhakim , El-Sayed R. El-Sayed","doi":"10.1016/j.procbio.2024.12.008","DOIUrl":"10.1016/j.procbio.2024.12.008","url":null,"abstract":"<div><div>Several endophytic fungal species were isolated and tested for their biosynthetic capabilities of Cu-NPs and Ni-NPs, as a potentially green and simple method with low cost. A promising fungus <em>Aspergillus terreus</em> was found to synthesize the two types of NPs. The synthesis of Cu-NPs and Ni-NPs was confirmed by several techniques. The antibacterial activity of the synthesized NPs alone and in combinations with different antibiotics (Benzathine penicillin and Ceftriaxone) against some antibiotic-resistant bacterial strains was assessed. The results showed that the antibiotic resistance genes of the tested bacterial strains were expressed when treated with either antibiotics, Cu-NPs, or Ni-NPs alone. Otherwise, it were down-regulated when treated with a combination of NPs and antibiotics, indicating the synergistic and additive effects of such combinations. Moreover, the cytotoxic activities of Cu-NPs and Ni-NPs were evaluated, and the results confirmed their moderate safety margin against several cancer and normal cell lines. Finally, the synthesis efficiency of both NPs was optimized using the response surface methodology. Under optimal conditions, the synthesis efficiency was significantly enhanced reaching 85.08 (Cu-NPs) and 80.71 % (Ni-NPs). These findings indicate that the fungal strain is a promising biofactory for Cu-NPs and Ni-NPs with promising activities to combat antibiotic-resistant bacteria and cancer.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"149 ","pages":"Pages 192-203"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143139503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pathogen-targeting bismuth nanocluster via photothermally-mediated NDM-1 inactivation and bacterial membrane destabilization combat NDM-1-producing bacteria

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-02-01 DOI: 10.1016/j.procbio.2024.12.011

Xichan He , Shaobing Liu , Jianan Tong , Yuhui Qiao , Xue Li , Kaixuan Dong , Dongsheng Yu , Yanshu Gu

{"title":"Pathogen-targeting bismuth nanocluster via photothermally-mediated NDM-1 inactivation and bacterial membrane destabilization combat NDM-1-producing bacteria","authors":"Xichan He , Shaobing Liu , Jianan Tong , Yuhui Qiao , Xue Li , Kaixuan Dong , Dongsheng Yu , Yanshu Gu","doi":"10.1016/j.procbio.2024.12.011","DOIUrl":"10.1016/j.procbio.2024.12.011","url":null,"abstract":"<div><div>New Delhi metallo-β-lactamase-1 (NDM-1) is the most prevalent type of metallo-β-lactamase and hydrolyzes almost all clinically used β-lactam antibiotics, including last line antibiotic - carbapenems. Inactivating NDM-1 and then reviving carbapenems holds great promise in treating NDM-1-producing bacteria, while better intracellular antibiotic accumulation and precise NDM-1 inactivation are challenges. Herein, a photothermal-assisted biomimetic antibiotic booster (PBM) is engineered for repurposing carbapenems against NDM-1-producing bacteria by photothermal-induced bacterial membrane destabilizing and synchronous NDM-1 inactivation. In the PBM nanomedicine, stomach medicine-converted bismuth nanoclusters (BiNCs) with excellent photothermal effect are used as photothermal skeleton, loading meropenem (MEM) on the inside and coating platelet membrane vesicles (PMVs) on the outside. Due to the inherent inflammatory properties of platelets, PBM exhibits an excellent homing effect on infectious sites and precise homologous targeting of pathogens. Meanwhile, due to the excellent photothermal properties of bismuth nanoclusters, PBM enhanced the intracellular accumulation of meropenem via breaking the bacterial outer membrane barrier, and then effectively inactivated NDM-1. Benefiting from the combination of photothermal-assisted bacterial membrane destabilizing and NDM-1 inactivation, PBM (100 μg/mL) effectively reversed the resistance of NDM-1-producing <em>Escherichia coli</em> to meropenem, exhibiting a more significant antibacterial effect (sterilization rate reached 98.9 %) than equivalent meropenem alone by time-killing curve.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"149 ","pages":"Pages 158-168"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143139252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and characterization of novel D-lyxose isomerases from the goat rumen metagenome

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-02-01 DOI: 10.1016/j.procbio.2024.12.014

Xin Xu , Mingyi Chen , Yali Su , Jiayun Ding , Xiuye Xie , Xinyi Zhang , Feng Li , Xian’ai Shi , Guozeng Wang

{"title":"Identification and characterization of novel D-lyxose isomerases from the goat rumen metagenome","authors":"Xin Xu , Mingyi Chen , Yali Su , Jiayun Ding , Xiuye Xie , Xinyi Zhang , Feng Li , Xian’ai Shi , Guozeng Wang","doi":"10.1016/j.procbio.2024.12.014","DOIUrl":"10.1016/j.procbio.2024.12.014","url":null,"abstract":"<div><div>Biocatalytic technology has emerged as a powerful tool for synthesizing functional sugars with a wide range of physiological functions. D-lyxose isomerase is an important aldose–ketose isomerase used for synthesizing functional sugars such as L-ribose and D-mannose; however, at present, only a few D-lyxose isomerases have been studied. In this study, a metagenomic approach was employed to mine novel D-lyxose isomerases from the goat rumen microbiome. Eleven full-length D-lyxose isomerase genes were identified using sequence alignment and phylogenetic analysis. All 11 genes were classified as Group Ⅱ D-lyxose isomerases and display low similarity to previously characterized D-lyxose isomerases. Five of the genes were selected for heterologous expression in <em>Escherichia coli</em>, and all were expressed successfully and had detectable enzyme activity. Further characterization revealed that optimum temperatures for the five enzymes range from 45 °C to 60 °C, and the optimum pH range is 7.0–8.0, with high relative residual activities under weakly acidic conditions. The recombinant enzymes have a broad substrate spectrum and are active against L-ribose, L-ribulose, D-fructose, and D-mannose, in addition to exhibiting the highest activity against D-lyxose. Among the five enzymes, GR-LI2 displays the highest activity toward L-ribulose, and GR-LI5 displays the highest activity against fructose, suggesting that these two enzymes have significant potential for L-ribose and D-mannose synthesis, respectively.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"149 ","pages":"Pages 213-221"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143139661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comprehensive review on integration of cellular metabolic engineering and cell-free systems for microbial platforms

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-02-01 DOI: 10.1016/j.procbio.2024.12.010

Arunangshu Das , Anita Verma , Naba Hazarika

{"title":"A comprehensive review on integration of cellular metabolic engineering and cell-free systems for microbial platforms","authors":"Arunangshu Das , Anita Verma , Naba Hazarika","doi":"10.1016/j.procbio.2024.12.010","DOIUrl":"10.1016/j.procbio.2024.12.010","url":null,"abstract":"<div><div>In response to the demand for environment friendly synthesis of chemical feedstocks, two disciplines have emerged: cellular metabolic engineering (CME) and cell-free metabolic engineering (CFME). Cell free systems largely replicate cellular pathways <em>in vitro</em> to bypass the need of live cells to achieve greater control and process simplicity. However, not all cellular biochemical aspects can be replicated <em>in vitro</em>, as some are not hardwired but crucial modulators of biochemical processes. These include metabolic states defined by ensemble of small molecules that influence proteostasis, catalytic activity of enzymes, and redox power, influencing cellular anabolic and catabolic decisions. Despite the advancement of molecular biology techniques engineering such control systems remains largely a challenge for cellular metabolic engineering. This review thoroughly examines these limitations in both fields and explores the potential for implementing non-hardwired control systems in cell-free metabolic engineering, either independently or in combination with cellular metabolic engineering. Further, the integration of chemistry and machine learning models is considered, with a focus on how their combined strengths can be leveraged to develop novel synthetic schemes.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"149 ","pages":"Pages 222-236"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143139495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The start-up of red blood cell-like Anammox granular sludge based on substrate volume flux

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-02-01 DOI: 10.1016/j.procbio.2024.11.035

Wenqiang Wang , Li Wang , Qu Wang , Yang Su , Jun Li , Dong Li

{"title":"The start-up of red blood cell-like Anammox granular sludge based on substrate volume flux","authors":"Wenqiang Wang , Li Wang , Qu Wang , Yang Su , Jun Li , Dong Li","doi":"10.1016/j.procbio.2024.11.035","DOIUrl":"10.1016/j.procbio.2024.11.035","url":null,"abstract":"<div><div>Enhancing substrate transfer is crucial for promoting the nitrogen removal rate of Anammox granular sludge, as the shape of the granules significantly influences substrate diffusion. In this study, inspired by biomimicry principles, we propose a novel red blood cell-like granular sludge to increase the contact area and improve substrate transfer. The startup and operation of Anammox granular sludge in low-strength ammonia nitrogen wastewater were successfully achieved through an increase in substrate volume flux (SVF). As the SVF increased from 2.38 to 7.43 L²/(g VSS·h), specific Anammox activity (SAA) rose from 0.138 to 0.483 g N/(g VSS·d). The Anammox granular sludge system achieved a total nitrogen removal efficiency of 85.70 % and a nitrogen removal rate (NRR) of 0.792 kg N/(m³·d) after 126 days. Notably, red blood cell-like granular sludge was observed by day 116. The rise in SVF induced a granule structure more conducive to substrate transport, improving the granules' efficiency in substrate uptake and thereby enhancing their SAA. High-throughput pyrosequencing revealed that the dominant bacteria using the SVF-enhancing strategy remained anaerobic ammonium oxidizing bacteria (AnAOB) (29.53 %-29.89 %), with an increased diversity of species, including <em>Candidatus Kuenenia</em>, <em>Candidatus Anammoxoglobus</em>, and <em>Candidatus Brocadia</em>.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"149 ","pages":"Pages 121-127"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143139498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasound-assisted deep eutectic solvent extraction of flavonoids from Hippophae plants: Optimization and α-glucosidase inhibition studies

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-01-31 DOI: 10.1016/j.procbio.2025.01.027

Yuyuan Huang , Hong Yang , Ying Zhang , Tongtong Zhang , Xiaoqiang Chen

{"title":"Ultrasound-assisted deep eutectic solvent extraction of flavonoids from Hippophae plants: Optimization and α-glucosidase inhibition studies","authors":"Yuyuan Huang , Hong Yang , Ying Zhang , Tongtong Zhang , Xiaoqiang Chen","doi":"10.1016/j.procbio.2025.01.027","DOIUrl":"10.1016/j.procbio.2025.01.027","url":null,"abstract":"<div><div>To make full use of <em>Hippophae</em> plant resources, we use the green extraction method of deep eutectic solvent (DES) combined with ultrasonication to extract flavonoids. HPLC was used to measure four flavonoids (isorhamnetin-3-<em>O</em>-glucoside-7-<em>O</em>-rhamnoside, rutin, isorhamnetin-3-<em>O</em>-rutinoside, and isorhamnetin) in fruit samples and five flavonoids (quercetin-7-<em>O</em>-glucoside, isorhamnetin-3-<em>O</em>-glucoside-7-<em>O</em>-rhamnoside, rutin, guaijaverin, and kaempferol-3-<em>O</em>-rutinoside) in leaf samples. The best solvent was chosen from 12 kinds of DES. With betaine/acetic acid (molar ratio of 1:2) as the extraction solvent, a liquid-solid ratio of 21:1 (mL/g), an extraction temperature of 37 °C, and an extraction time of 22 min, the total content of four flavonoids in the fruit was 10.547 mg/g. With L-proline/levulinic acid (molar ratio of 1:2) as the extraction solvent, a liquid-solid ratio of 31:1 (mL/g), an extraction temperature of 49 °C, and an extraction time of 22 min, the yield of five flavonoids in the leaves was 17.921 mg/g. The extraction efficiency of this method was significantly better than that of hot reflux extraction and 60 % ethanol extraction (<em>p</em> < 0.05). The flavonoids obtained by further purification of the extracts from the fruit and leaves exhibited inhibitory ability to α-glucosidase in <em>vitro</em>, IC<sub>50</sub> = 28.189 and 19.675 μg/mL, respectively. Above all, the use of optimized DES could efficiently extract flavonoids from <em>Hippophae</em> plants, which have promising applications in the regulation of blood glucose.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"151 ","pages":"Pages 43-51"},"PeriodicalIF":3.7,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143271148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in enzyme-based metal nanoparticles as colorimetric biosensors for the detection of pesticides: A review

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-01-30 DOI: 10.1016/j.procbio.2025.01.028

Nor Khairun Bariah Kamaruddin , Shahrul Ainliah Alang Ahmad , Anis Abdul Ghani

{"title":"Recent advances in enzyme-based metal nanoparticles as colorimetric biosensors for the detection of pesticides: A review","authors":"Nor Khairun Bariah Kamaruddin , Shahrul Ainliah Alang Ahmad , Anis Abdul Ghani","doi":"10.1016/j.procbio.2025.01.028","DOIUrl":"10.1016/j.procbio.2025.01.028","url":null,"abstract":"<div><div>Pesticides residues determination has received significant interest due to the high acute toxicity and their potential to cause long-term damage to the environment and human lives. Traditional analytical methods such as HPLC and GC-MS have been widely employed to analyse pesticides in various samples. However, these methods have drawbacks such as tedious sample preparation, complexity, expensive, and the need for highly specialized staff. As a result, there is an increasing demand for analytical methods that enable easy, quick, sensitive, selective, low-cost, and reliable detection of pesticides at trace levels. Enzyme-based biosensors offer a promising alternative, providing rapid, sensitive, and cost-effective pesticide detection. Recent advancements in colorimetric biosensors using metal nanoparticles enable on-site monitoring visible by the naked eye. These innovations may lead to portable instruments for rapid toxicity testing, revolutionizing pesticide analysis. This paper reviews insecticide types, effects, challenges, and the development of metal nanoparticle-based colorimetric biosensors for identifying pesticide.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"151 ","pages":"Pages 99-112"},"PeriodicalIF":3.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143378886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reaction pathways and integral kinetics for hydrolysis of soybean oligosaccharides by α-galactosidase and invertase

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-01-30 DOI: 10.1016/j.procbio.2025.01.021

Md Fauzul Kabir , Calum Bochenek , Chrys Wesdemiotis , Lu-Kwang Ju

{"title":"Reaction pathways and integral kinetics for hydrolysis of soybean oligosaccharides by α-galactosidase and invertase","authors":"Md Fauzul Kabir , Calum Bochenek , Chrys Wesdemiotis , Lu-Kwang Ju","doi":"10.1016/j.procbio.2025.01.021","DOIUrl":"10.1016/j.procbio.2025.01.021","url":null,"abstract":"<div><div>The increasingly produced soybean molasses lacks value-added applications because its oligosaccharides stachyose and raffinose are poorly digestible/hydrolysable by many organisms. Enzymatic upgrading can be effective, but no study has previously examined and modeled its full complexity. This study addresses this knowledge gap by developing comprehensive models and evaluating them with results of enzymatic hydrolysis of, progressively, pure sucrose, melibiose, raffinose and stachyose, and then soybean molasses. The models considered all possible reaction pathways, competitive inhibition among substrates and intermediates, and kinetic decline of enzyme activities. The kinetic parameters, obtained from regression of concentration profiles for all carbohydrates (substrate, intermediates, and products), offered important insights into enzymatic hydrolysis of soybean galacto-oligosaccharides. Most importantly, digalactose was, for the first time, shown to form appreciably during stachyose hydrolysis (by breakage of mid-structure α-1,6 galactose-glucose bond), with rates comparable to the commonly recognized raffinose formation (by cleavage of terminal galactose). On the other hand, the invertase-catalyzed breakdown of stachyose to manninotriose (+ fructose) was much less significant. Further, the <em>A. niger</em> invertase could remove fructose from sucrose, raffinose, and stachyose, but with substantially different effectiveness: sucrose > raffinose > stachyose. Practically, the models developed are valuable to the development of enzymatic soybean-molasses upgrading process.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"150 ","pages":"Pages 357-370"},"PeriodicalIF":3.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143094485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0