Process Biochemistry最新文献_第4页

The optimized signal peptide and feeding strategy to enhance secretory expression of LegH in Komagataella phaffii 优化信号肽及摄食策略对提高法菲小鹿LegH分泌表达的影响

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-09 DOI: 10.1016/j.procbio.2025.06.005

Hongtao Chen , Yueheng Niu , Liuxia Zhang , Yunpeng Wang , Ao Luo , Zhihui Dai , Guocheng Du , Xinrui Zhao

引用次数: 0

Nutraceuticals enhanced by nanotechnology: A new frontier for obesity treatment 纳米技术增强的营养保健品：肥胖症治疗的新前沿

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-06 DOI: 10.1016/j.procbio.2025.05.021

Sri Renukadevi Balusamy , Selvakumar Vijayalakshmi , Sumathi Sundaravadivelu , Md Amdadul Huq , Sagnik Nag , Sourav Mohanto , Johan Sukweenadhi , Deong Hwan Oh , Haribalan Perumalsamy

{"title":"Nutraceuticals enhanced by nanotechnology: A new frontier for obesity treatment","authors":"Sri Renukadevi Balusamy , Selvakumar Vijayalakshmi , Sumathi Sundaravadivelu , Md Amdadul Huq , Sagnik Nag , Sourav Mohanto , Johan Sukweenadhi , Deong Hwan Oh , Haribalan Perumalsamy","doi":"10.1016/j.procbio.2025.05.021","DOIUrl":"10.1016/j.procbio.2025.05.021","url":null,"abstract":"<div><div>Nutraceuticals encompass bioactive compounds like polyphenols, flavonoids, and essential fatty acids inherent in food that play defensive roles against human ailments. Presently, the pharmaceutical industry is focused on nutraceutical-based nanomaterials to mitigate drawbacks in obesity treatment. The use of nanomaterials enhances bioavailability, stability, and targeted drug delivery, diminishing side effects. This review delineates the significance, limitations, and molecular mechanisms of nutraceuticals' pharmacological properties, emphasizing how nutraceutical-based nanomaterials address challenges for effective obesity treatment. We outline potential nano-based anti-obesity drugs for heightened pharmacological impact and acknowledge associated clinical challenges and limitations. Critically, we evaluate the advantages and disadvantages of nutraceuticals, advocating various delivery systems to enhance their anti-obesity efficacy. Notably, nutraceutical-based nanoparticles offer increased bioavailability, precise distribution, reduced side effects, and prolonged sustainability, revolutionizing obesity management. Conscious implementation of nutraceutical-based nanoparticles holds promise in alleviating complications associated with conventional nutraceuticals in obesity management.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 217-235"},"PeriodicalIF":3.7,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144240181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of ultrasound-assisted pH shift treatment on structure and functional properties of mulberry leaf protein 超声辅助pH转移处理对桑叶蛋白结构和功能特性的影响

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-06 DOI: 10.1016/j.procbio.2025.06.004

Ke Li , Qiongying Wu , Junqiang Jia

{"title":"Effect of ultrasound-assisted pH shift treatment on structure and functional properties of mulberry leaf protein","authors":"Ke Li , Qiongying Wu , Junqiang Jia","doi":"10.1016/j.procbio.2025.06.004","DOIUrl":"10.1016/j.procbio.2025.06.004","url":null,"abstract":"<div><div>This study investigated the impact of multifrequency ultrasound (22/28/40 kHz)-assisted pH shift treatment on the structural and functional attributes of mulberry leaf protein (MLP). The results showed that ultrasound-assisted pH shift increased the solubility of MLP by 281.74 % and significantly increased its emulsifying activity index (from 188.63 ± 3.27–231.41 ± 5.22 m²/g), emulsion stability index (from 51.22 ± 2.67–136.20 ± 9.98) and in vitro protein digestibility (from 41.75 % ± 3.57–72.54 % ± 1.38 %). The reduction in MLP particle size and the increase in zeta potential indicated that the treatment disrupted the tertiary structure. Furthermore, the increases in ultraviolet absorbance, fluorescence intensity, and molecular flexibility revealed significant alterations in the secondary and tertiary structures induced by ultrasound-assisted pH shift. The treatment reduced the α-helix content, while increased the β-sheet content, suggesting partial unfolding in the secondary structure of MLP, which led to the increase of exposed hydrophobic groups. As a result, the surface hydrophobicity of MLP was increased after ultrasound-assisted pH shift treatment. In conclusion, ultrasound-assisted pH shift treatment holds promise for advancing the application of MLP in the food industry.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 244-254"},"PeriodicalIF":3.7,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144262486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Purification of messenger RNA directly from crude IVT using polyethylene glycol and NaCl precipitation 用聚乙二醇和NaCl沉淀法直接从粗IVT中纯化信使RNA

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-06 DOI: 10.1016/j.procbio.2025.06.002

Maria del Carme Pons Royo , Tyler Arnold , Isabella Perez Rodriguez , Nicole Ostrovsky , Mushriq Al-Jazrawe , Andrew Hatas , Vico Tenberg , Allan S. Myerson , Richard D. Braatz

{"title":"Purification of messenger RNA directly from crude IVT using polyethylene glycol and NaCl precipitation","authors":"Maria del Carme Pons Royo , Tyler Arnold , Isabella Perez Rodriguez , Nicole Ostrovsky , Mushriq Al-Jazrawe , Andrew Hatas , Vico Tenberg , Allan S. Myerson , Richard D. Braatz","doi":"10.1016/j.procbio.2025.06.002","DOIUrl":"10.1016/j.procbio.2025.06.002","url":null,"abstract":"<div><div>The increasing demand for mRNA-based therapeutics requires scalable and cost-effective purification methods. Chromatography-based approaches, such as Oligo-dT affinity chromatography, require multiple processing steps, including buffer exchange and high-temperature treatments, which can lead to mRNA degradation and increased costs. Although precipitation is commonly used at the lab scale, its application in large-scale mRNA purification remains underexplored. In this study, we developed a rapid and efficient precipitation-based method for mRNA purification. The effects of different precipitation conditions, including salt type, co-precipitating agents, and time, were evaluated in terms of recovery yield and purity. Precipitation experiments were conducted for two different in vitro transcription crude materials containing mRNAs of varying lengths and concentrations. The optimal conditions, identified as a combination of NaCl and PEG 6000, achieved recovery yields of 80–93 % and purities of 80–83 %, with no detectable double-stranded RNA formation or fragmentation. The precipitated mRNAs were successfully transfected into 293 T and A549 cells, showing protein expression comparable to commercial and chromatography-purified mRNAs. These findings position PEG/NaCl precipitation as a rapid, scalable, and cost-effective alternative to traditional chromatography methods, significantly reducing process complexity and time for large-scale mRNA production.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 263-273"},"PeriodicalIF":3.7,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144262487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Static magnetic field enhances the purification of gaseous n-hexane in airlift two-phase partitioning bioreactor 静磁场增强了气升式两相分配生物反应器中气态正己烷的净化效果

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-06 DOI: 10.1016/j.procbio.2025.06.003

Qian Li , Xianhao Lu , Keping Zhang , Xianwang Kong , Lichao Lu , Jianmeng Chen , Dongzhi Chen

{"title":"Static magnetic field enhances the purification of gaseous n-hexane in airlift two-phase partitioning bioreactor","authors":"Qian Li , Xianhao Lu , Keping Zhang , Xianwang Kong , Lichao Lu , Jianmeng Chen , Dongzhi Chen","doi":"10.1016/j.procbio.2025.06.003","DOIUrl":"10.1016/j.procbio.2025.06.003","url":null,"abstract":"<div><div>Two-phase partitioning bioreactor (TPPB) has been developed as a promising approach to improve the biological purification of hydrophobic waste-gas. However, the biodegradation efficiency of highly hydrophobic pollutants remains limited. Herein, static magnetic field (SMF) was innovatively introduced to enhance the biodegradation of the model pollutant <em>n</em>-hexane and the underlying mechanism was revealed. Results showed that the influence of SMF on different <em>n</em>-hexane degrading bacteria varied significantly, and SMF cast the most pronounced enhancement on <em>Pseudomonas</em> HY-4 under the optimized intensity of 20 mT. The removal efficiency of <em>n</em>-hexane with an inlet concentration of 540·mg m<sup>−3</sup> using the SMF enhanced TPPB reactor reached 93 % within 5 days, surpassing the unenhanced bioreactor (82 %). And the SMF enhanced reactor retained the original shock load resistance. Mechanism analysis revealed that, under the action of SMF, the microbial cell surface hydrophobicity was sustainably improved, thereby improving the mass transfer of <em>n</em>-hexane diffusing from the liquid to the microbial phase. The electron transport chain activity in the SMF-enhanced TPPB was enhanced by 10.3 %. Furthermore, the abundance of degrading bacteria beneficial to <em>n</em>-hexane degradation was greatly enriched. This work may lay a foundation for the application of SMF in enhancing the biodegradation of hydrophobic gaseous pollutants.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 209-216"},"PeriodicalIF":3.7,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144241090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combinatorial engineering of microbial cell factory for 2′-fucosyllactose biosynthesis 2′-焦酰基乳糖生物合成微生物细胞工厂的组合工程

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-04 DOI: 10.1016/j.procbio.2025.05.024

Jingyu Zhou , Xinyue Su , Yongzhong Wang , Qiang Ding

{"title":"Combinatorial engineering of microbial cell factory for 2′-fucosyllactose biosynthesis","authors":"Jingyu Zhou , Xinyue Su , Yongzhong Wang , Qiang Ding","doi":"10.1016/j.procbio.2025.05.024","DOIUrl":"10.1016/j.procbio.2025.05.024","url":null,"abstract":"<div><div>2’-fucosyllactose (2’-FL), a predominant human milk oligosaccharide (HMOs), has showed significant potential in infant health applications including intestinal protection, cognitive development, and immune system modulation. Therefore, to enhance microbial biosynthesis of 2’-FL, we engineered a de novo synthesis pathway through deleting key enzymes gene: <em>wecB</em> (encoding UDP-N-acetyl glucosamine-2-epimerase), <em>lacZ</em> (encoding β-galactosidase), <em>wcaJ</em> (encoding UDP-glucose lipid carrier transferase), which could increase the 2’-FL titer and productivity to 2.25 g/L and 0.03 g/L/h, respectively. Furthermore, optimization of induction time further improved production performance to 4.06 g/L and 0.06 g/L/h. Through systematic gene deletion analysis, we identified <em>lacA</em> and <em>iclR</em> as critical metabolic nodes. Their knockout negatively impacted 2’-FL biosynthesis by reducing lactose availability and disturbing metabolic flux. Finally, the 2’-FL was furthermore improved to 4.75 g/L through expressing <em>gsk</em> and <em>zwf</em> genes for NADPH and GTP regeneration. This study can effectively increase the production of 2’-fucosyllactose, which can provide a research basis for the large-scale bioproduction of 2’-fucosyllactose in the industrial factory.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 202-208"},"PeriodicalIF":3.7,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144230402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tannic acid degradation potential and biochemical characterization of Paenibacillus lautus BCA501 isolated from the gut of Silver therapon (Leiopotherapon plumbeus) 银鲢肠道中劳氏芽孢杆菌BCA501单宁酸降解潜力及生化特性研究

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-04 DOI: 10.1016/j.procbio.2025.05.022

Nico G. Dumandan , Christian James B. Raiz , Annie Cita T. Kagaoan , Leo Joseph F. Labitag , Jay Ronel V. Conejos , Francisco B. Elegado , Amelia B. Hizon-Fradejas , Annabelle T. Abrera , Sheryl Lozel B. Arreola

{"title":"Tannic acid degradation potential and biochemical characterization of Paenibacillus lautus BCA501 isolated from the gut of Silver therapon (Leiopotherapon plumbeus)","authors":"Nico G. Dumandan , Christian James B. Raiz , Annie Cita T. Kagaoan , Leo Joseph F. Labitag , Jay Ronel V. Conejos , Francisco B. Elegado , Amelia B. Hizon-Fradejas , Annabelle T. Abrera , Sheryl Lozel B. Arreola","doi":"10.1016/j.procbio.2025.05.022","DOIUrl":"10.1016/j.procbio.2025.05.022","url":null,"abstract":"<div><div>Microbial degradation of tannic acid offers significant potential for mitigating the negative effects of tannins while enabling various biotechnological applications. In this study, <em>Paenibacillus lautus</em> BCA501, isolated from the gut of the Silver therapon (<em>Leiopotherapon plumbeus</em>), is reported for the first time as a tannic acid-degrading bacterium. The strain effectively utilized tannic acid for growth, achieving a biomass yield coefficient (Y<sub>X/S</sub>) of 1.30 g<sub>biomass</sub> per g<sub>substrate</sub> at 2 g/L tannic acid, which decreased to 0.594 g<sub>biomass</sub> per g<sub>substrate</sub> at 10 g/L. Kinetic analysis revealed a maximum growth rate of 0.562h<sup>-1</sup>, with substrate inhibition at 11.26 g/L based on the Monod model. Tannase activity, though growth-associated, declined from 0.300 to 0.046 U/mL with increasing substrate concentration. Despite this reduction, gallic acid release increased significantly, with its growth-associated production coefficient peaking at 2.22 g/L at 10 g/L tannic acid, demonstrating efficient hydrolysis into phenolic compounds. This study provides the first evidence of tannic acid degradation by <em>Paenibacillus lautus</em> underscoring its potential for microbial tannase production and phenolic biotransformation.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 236-243"},"PeriodicalIF":3.7,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144241089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Progress in chitosan-based materials: Enhancing edible coatings and films through modifications and functionalization for food preservation 壳聚糖基材料的进展：通过改性和功能化来增强可食用涂层和薄膜用于食品保鲜

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-03 DOI: 10.1016/j.procbio.2025.06.001

Maricarmen Iñiguez-Moreno , Josué David Hernández-Varela , Manuel Burelo , Joel H. Elizondo-Luevano , Rafael G. Araújo , Cecilia D. Treviño-Quintanilla , Dora I. Medina

{"title":"Progress in chitosan-based materials: Enhancing edible coatings and films through modifications and functionalization for food preservation","authors":"Maricarmen Iñiguez-Moreno , Josué David Hernández-Varela , Manuel Burelo , Joel H. Elizondo-Luevano , Rafael G. Araújo , Cecilia D. Treviño-Quintanilla , Dora I. Medina","doi":"10.1016/j.procbio.2025.06.001","DOIUrl":"10.1016/j.procbio.2025.06.001","url":null,"abstract":"<div><div>Chitosan has emerged as a versatile biopolymer with significant potential in food technology, particularly for developing edible coatings aimed at preserving perishable foods. This review highlights recent advancements in chitosan extraction, modification, and functionalization, emphasizing its applications in extending the shelf life of fruits, vegetables, meat, seafood, and dairy products. Chitosan-based coatings effectively reduce moisture, oxygen, and carbon dioxide permeability, thereby delaying oxidation and respiration. Their functionality can be further enhanced by incorporating nanomaterials and bioactive compounds, which improve mechanical strength, antimicrobial efficacy, and antioxidant activity while preserving the physicochemical and sensory quality of food. The development of smart chitosan coatings, responsive to changes in pH, temperature, and humidity, offers innovative possibilities for real-time food quality monitoring. Although chitosan is generally recognized as safe (GRAS), long-term toxicity studies and regulatory harmonization are necessary to ensure consumer safety. Moreover, alternative sources such as fungal-derived chitosan present sustainable, hypoallergenic options that help address the limitations of traditional crustacean-based chitosan. Overall, the use of chitosan in food preservation not only supports improved product quality and safety but also contributes to environmentally responsible and health-conscious packaging approaches, aligning with current sustainability goals in the food industry.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 175-190"},"PeriodicalIF":3.7,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144205772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of high hydrostatic pressure on Alcalase-assisted hydrolysis of soy protein isolate and the anti-aging activity of the hydrolysates 高静水压力对alcalase辅助水解大豆分离蛋白及其抗衰老活性的影响

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-01 DOI: 10.1016/j.procbio.2025.05.020

Xiaorui Liu , Lidan Pan , Yaxin Cheng , Die Dong , Haibo Zhao , Xiuzhen Ding , Chao Yuan , Bo Cui

{"title":"Effect of high hydrostatic pressure on Alcalase-assisted hydrolysis of soy protein isolate and the anti-aging activity of the hydrolysates","authors":"Xiaorui Liu , Lidan Pan , Yaxin Cheng , Die Dong , Haibo Zhao , Xiuzhen Ding , Chao Yuan , Bo Cui","doi":"10.1016/j.procbio.2025.05.020","DOIUrl":"10.1016/j.procbio.2025.05.020","url":null,"abstract":"<div><div>In this study, soy protein isolate hydrolysates (SPIH) with enhanced antiaging activity were produced. SPIH were generated by Alcalase hydrolysis of soy protein isolate (SPI) pretreated under high hydrostatic pressure (HHP: 200–500 MPa, 5–15 min). Results showed HHP treatment led to the transition of secondary structures of SPI, withα-helix and β-sheet transforming into random coil and β-turn. The dense network structure of SPI was disrupted, increasing the hydrolysis degree by 19.2 % (300 MPa, 5 min). Attributed to the radical scavenging effect by the surface exposure of hydrophobic residues, the antioxidant activity of HHP-treated SPIH were increased and reached a peak under the condition of 300 MPa for 5 min. In yeast cell anti-aging assays, HHP-treated SPIH decreased the accumulation of reactive oxygen species and malondialdehyde content, thereby delaying cellular aging. These findings demonstrated that HHP-assisted enzymatic hydrolysis enhanced the yield and bioactivity of anti-aging ingredients derived from SPI, providing a novel strategy for developing functional food.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 191-201"},"PeriodicalIF":3.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144203957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

On digital twins in bioprocessing: Opportunities and limitations 生物加工中的数字孪生：机遇与局限

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-05-31 DOI: 10.1016/j.procbio.2025.05.023

Mehrdad Shariatifar , Mohammadsadegh Salimian Rizi , Rahmat Sotudeh-Gharebagh , Reza Zarghami , Navid Mostoufi

{"title":"On digital twins in bioprocessing: Opportunities and limitations","authors":"Mehrdad Shariatifar , Mohammadsadegh Salimian Rizi , Rahmat Sotudeh-Gharebagh , Reza Zarghami , Navid Mostoufi","doi":"10.1016/j.procbio.2025.05.023","DOIUrl":"10.1016/j.procbio.2025.05.023","url":null,"abstract":"<div><div>Integrating Digital Twins (DTs) in bioprocessing has become a prominent focus within the industry. Despite the challenges associated with implementing this technology in the field, the bioprocessing sector is interested in utilizing it. This is due to its potential to enhance process efficiency and overall profitability. The adoption of DTs is driven by the prospect of online monitoring, control, and optimization, enabling the products with precise and desired characteristics. To realize this objective, researchers propose a novel strategy for implementing DTs in bioprocessing. This involves the development of a hybrid model that combines first principal models and Machine Learning (ML) algorithms. This approach effectively addresses the limitations of previous methods and establishes a closed control loop system, continuously monitoring the system and adjusting input variables to achieve optimal outcomes. This study comprehensively explores various aspects of DTs. Firstly, it discusses the concept and characteristics of DTs, along with an examination of the advantages and challenges associated with their implementation. Secondly, it comprehensively analyzes key factors that directly influence DT implementation, including sensors, data collection, and models. Thirdly, it reviews the implications of Digital Solutions (DS) and DT in downstream and upstream bioprocessing. By providing theories, case studies, and practical frameworks, this work seeks to motivate both researchers and industry practitioners to adopt DT methodologies, thereby facilitating the emergence of enhanced precision, operational efficiency, and economic viability within biomanufacturing.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 274-299"},"PeriodicalIF":3.7,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144270006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0