Process Biochemistry最新文献_第4页

Enhanced biodegradation of diesel and phenol by acclimatized freshwater microalgae: A study on adaptation and degradation efficiency 驯化淡水微藻增强柴油和苯酚的生物降解：适应性和降解效率的研究

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-03-01 Epub Date: 2026-01-20 DOI: 10.1016/j.procbio.2026.01.015

Prashant Sinha, Suparna Mukherji

{"title":"Enhanced biodegradation of diesel and phenol by acclimatized freshwater microalgae: A study on adaptation and degradation efficiency","authors":"Prashant Sinha, Suparna Mukherji","doi":"10.1016/j.procbio.2026.01.015","DOIUrl":"10.1016/j.procbio.2026.01.015","url":null,"abstract":"<div><div>A freshwater algal culture was obtained from rock surfaces near Powai Lake and incubated at 30 ± 5⁰C under illumination of 1500 ± 200 lux with light:dark cycle of 16:8 h in Bold Basal medium and BG-11 medium. The culture was acclimatized to diesel (0.6 % v/v) and phenol (200 mg/L) over 15–20 days to enhance its metabolic capacity for biodegradation. Acclimatization reduced the lag phase and increased the chlorophyll-a concentration at the end of log phase, indicating improved growth and pollutant tolerance. Growth and degradation studies were conducted using different initial concentrations of diesel and phenol under static conditions. The acclimatized culture exhibited significant biodegradation capabilities. Diesel biodegradation efficiency over and above the control (D<sub>max</sub>) was found to be 33.3 % for 0.2 % (v/v) diesel and 37.4 % for 0.6 % (v/v) diesel over 25 days for 100 mL cultivation volume. Phenol degradation was rapid and complete degradation was observed within 3 days for initial concentration up to 400 mg/L. Metagenomic analysis identified cyanobacteria as the dominant phylum, with <em>Leptolyngbya</em> as the most abundant genus. <em>Nostoc</em>, <em>Chroococcus</em>, and <em>Synechococcus</em> were also present in the consortium. This study demonstrates the potential of acclimatized freshwater algae for effective bioremediation of pollutants commonly present in refinery wastewater.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"162 ","pages":"Pages 149-158"},"PeriodicalIF":4.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146039130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of tetrahydrocurcumin-loaded hybrid calcium carbonate microcrystals for enhanced collagen synthesis 负载四氢姜黄素的杂化碳酸钙微晶促进胶原合成的评价

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-03-01 Epub Date: 2026-01-13 DOI: 10.1016/j.procbio.2026.01.008

Ee Taek Hwang , Yeahwa Yoon , Ka Ram Kim , Jinhee Jeong , Jung Soo Kim , Dongwon Kim , Seong-Wook Seo , In-Soo Yoon

{"title":"Evaluation of tetrahydrocurcumin-loaded hybrid calcium carbonate microcrystals for enhanced collagen synthesis","authors":"Ee Taek Hwang , Yeahwa Yoon , Ka Ram Kim , Jinhee Jeong , Jung Soo Kim , Dongwon Kim , Seong-Wook Seo , In-Soo Yoon","doi":"10.1016/j.procbio.2026.01.008","DOIUrl":"10.1016/j.procbio.2026.01.008","url":null,"abstract":"<div><div>Plant-derived natural product-encapsulated organic–inorganic hybrid materials have shown promise as biologically active agents for health. Here, we extend our previous work by investigating the collagen synthesis activity and stability of tetrahydrocurcumin (THC) in amphiphilic copolymer-incorporated calcium carbonate biomineral formulations, offering a novel comparison with curcumin (CUR). The encapsulated THC preserved over 90 % of its initial concentration over four weeks under different storage conditions, confirming its high stability. Interestingly, THC induced 1.58- and 1.55-fold higher COL1A1 expression in normal human dermal fibroblasts (NHDFs) than CUR, enhancing collagen synthesis–related gene expression when 500 and 1000 µg mL<sup>−1</sup> hybrid microcrystals were used. These findings contrast with general assumptions that CUR exhibits superior therapeutic effects, illustrating the challenges in predicting the effectiveness of encapsulation technologies. This is the first study to systematically compare the encapsulation and biological efficiencies of CUR and THC in organic–inorganic hybrid materials, contributing to the broader field of natural product-based therapeutics.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"162 ","pages":"Pages 53-61"},"PeriodicalIF":4.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145980656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An integrated hydrothermal-enzymatic biorefinery approach for efficient ferulic acid production from Euryale ferox seed shell via lignin removal 木质素去除法从芡实种子壳中高效生产阿魏酸的一体化水热-酶生物精炼方法

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-03-01 Epub Date: 2026-01-14 DOI: 10.1016/j.procbio.2026.01.013

Shiquan Qian , Xinran Cheng , Wenxing Song , Youyou Zheng , Ruojun Tang , Jingjing Fan , Ci Jin , Enjie Diao

{"title":"An integrated hydrothermal-enzymatic biorefinery approach for efficient ferulic acid production from Euryale ferox seed shell via lignin removal","authors":"Shiquan Qian , Xinran Cheng , Wenxing Song , Youyou Zheng , Ruojun Tang , Jingjing Fan , Ci Jin , Enjie Diao","doi":"10.1016/j.procbio.2026.01.013","DOIUrl":"10.1016/j.procbio.2026.01.013","url":null,"abstract":"<div><div>The effectiveness of combined hydrothermal and enzymic hydrolysis for ferulic acid recovery was investigated. Combined hydrothermal, cellulase, xylanase and ferulic acid esterase hydrolysis (HCXF) proved to be resulted in disruption of lignocellulose fibers and removal of hemicellulose and lignin in pretreated <em>Euryale ferox</em> seed shell, thus improved enzymatic saccharification. Interestingly, when performed using HCXF via hydrothermal hydrolysis (29.7 ml/g, 110 °C, 1.0 h) followed by enzymatic hydrolysis with 1:2:2 (v/v) of cellulase (31.3 ml/g, 1.0 %): xylanase (34.5 ml/g, 0.7 %): ferulic acid esterase (16.6 ml/g, 0.7 %) for 36 h, 2.21 mg/g of ferulic acid recovery and 30.82 % of lignin removal rate were obtained. Moreover, the ferulic acid extracts obtained by HCXF exhibited strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radicals scavenging effects, showing the IC<sub>50</sub> value at 0.19 and 0.11 mg/ml, respectively. HCXF process efficiently improved the recovery of ferulic acid through lignin removal in pretreated <em>E. ferox</em> seed shell, leading to having the potential application in food or pharmaceutical industry as antioxidant ingredients.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"162 ","pages":"Pages 74-82"},"PeriodicalIF":4.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145980659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Boosting Arthrospira platensis metabolism during biogas upgrading via carbon coated iron-based nanoparticle addition 通过添加碳包覆铁基纳米颗粒促进沼气升级过程中平台节螺旋体的代谢

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-03-01 Epub Date: 2026-01-12 DOI: 10.1016/j.procbio.2026.01.004

Chrysa Anagnostopoulou , Laura Vargas-Estrada , Panagiotis G. Kougias , Raúl Muñoz

{"title":"Boosting Arthrospira platensis metabolism during biogas upgrading via carbon coated iron-based nanoparticle addition","authors":"Chrysa Anagnostopoulou , Laura Vargas-Estrada , Panagiotis G. Kougias , Raúl Muñoz","doi":"10.1016/j.procbio.2026.01.004","DOIUrl":"10.1016/j.procbio.2026.01.004","url":null,"abstract":"<div><div>Carbon-coated iron-based nanoparticles have demonstrated a significant potential to enhance the performance of biological processes, particularly microalgal cultivation. In this study, the effect of two types of commercially available iron-based nanoparticles, namely CALPECH and SMALLOPS, was evaluated on <em>Arthrospira platensis</em> metabolism during biogas upgrading in batch cultures. Both nanoparticles enhanced CO<sub>2</sub> capture, O<sub>2</sub> production, cyanobacterial growth and phycocyanin synthesis across all tested concentrations. However, the highest phycocyanin content (180 mg·g<sup>−1</sup>) was reached by the addition of CALPECH nanoparticles at 100 mg·L<sup>−1</sup>. Further experiments under stress conditions, including increased light intensity (300 and 600 μmol·m<sup>−2</sup>·s<sup>−1</sup>) and salinity (0.1–0.5 M NaCl) confirmed the beneficial effects of CALPECH. At 600 μmol·m<sup>−2</sup>·s<sup>−1</sup> CALPECH nanoparticles enhanced biomass productivity and increased CO<sub>2</sub> capture by 33 % while maintaining phycocyanin content at 178 mg·g<sup>−1</sup>. Moreover, the addition of 100 mg·L<sup>−1</sup> of CALPECH nanoparticles at 0.1 M NaCl slightly improved biogas upgrading performance and increased phycocyanin content to 192.7 mg·g<sup>−1</sup>. In this context, increasing salinity to 0.5 M caused stress in <em>Arthrospira platensis</em> cells, reducing photosynthetic efficiency regardless of nanoparticle addition. These outcomes highlight the potential of carbon-coated iron-based nanoparticles to improve <em>Arthrospira platensis</em> growth and pigment production, which would ultimately increase the techno-economic feasibility of photosynthetic biogas upgrading.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"162 ","pages":"Pages 32-42"},"PeriodicalIF":4.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145980660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering a self-sufficient nitric oxide-generating module (BsNOS-YkuN) for versatile P450-catalyzed nitration platforms 设计一个自给自足的一氧化氮生成模块（BsNOS-YkuN），用于多功能p450催化硝化平台

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-02-01 Epub Date: 2025-11-17 DOI: 10.1016/j.procbio.2025.11.008

Haiying Lin , Yun Chen , Yijie Yu , Yanmei Wang , Jianru Pan

{"title":"Engineering a self-sufficient nitric oxide-generating module (BsNOS-YkuN) for versatile P450-catalyzed nitration platforms","authors":"Haiying Lin , Yun Chen , Yijie Yu , Yanmei Wang , Jianru Pan","doi":"10.1016/j.procbio.2025.11.008","DOIUrl":"10.1016/j.procbio.2025.11.008","url":null,"abstract":"<div><div>Nitrotryptophan is a high-value compound, yet its production via traditional chemical synthesis suffers from low selectivity and environmental concerns. Biocatalysis using P450 \"nitrases\" offers a promising green alternative, but its efficiency is often limited by the supply of the crucial co-substrate, nitric oxide (NO), often due to electron transfer limitations in NO synthases. To address this, we engineered a self-sufficient <em>Escherichia coli</em> biocatalyst based on a novel fusion enzyme, BsNOS-YkuN, which links a nitric oxide synthase with its essential reductase to ensure efficient electron transfer. This fusion biocatalyst exhibited superior specific activity (13.5 U/mg) and enabled a robust cell-free nitration system, yielding 86.5 mg/L of product after optimization. When implemented in a whole-cell system with the P450 nitrase TxtE-BM3R, this module proved highly effective. Systematic optimization of fermentation (25°C, pH 7.7) and a fed-batch strategy boosted the titer to 220.6 mg/L. Furthermore, deleting the competing gene <em>pheA</em> accelerated production from endogenous precursors. This work demonstrates a multi-layered engineering strategy, creating a versatile platform for nitroaromatic production proven in both in vitro and in vivo contexts.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"161 ","pages":"Pages 26-34"},"PeriodicalIF":4.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145570631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative biodegradation of lignite by Bacillus cereus and Lelliottia amnigena: Elucidating enzymatic, adhesion, and metabolic mechanisms through multi-technique analysis 蜡样芽孢杆菌和羊水小叶对褐煤的比较生物降解：通过多技术分析阐明酶、粘附和代谢机制

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-02-01 Epub Date: 2025-12-25 DOI: 10.1016/j.procbio.2025.12.014

Yaya Wang , Weilong Cao , Damir Nussipov , Yuan Bao , Jiaxuan Li , Zhiwei Shi , Anning Zhou , Kuanysh Tastambek , Xiangrong Liu

{"title":"Comparative biodegradation of lignite by Bacillus cereus and Lelliottia amnigena: Elucidating enzymatic, adhesion, and metabolic mechanisms through multi-technique analysis","authors":"Yaya Wang , Weilong Cao , Damir Nussipov , Yuan Bao , Jiaxuan Li , Zhiwei Shi , Anning Zhou , Kuanysh Tastambek , Xiangrong Liu","doi":"10.1016/j.procbio.2025.12.014","DOIUrl":"10.1016/j.procbio.2025.12.014","url":null,"abstract":"<div><div>China's abundant low-rank coal suffers from high moisture and low calorific value, limiting its energy efficiency. Biodegradation offers a sustainable quality enhancement strategy. This study assessed the lignite degradation capabilities of bacterial strains Bacillus cereus (ESM1) and Lelliottia amnigena (P1) using enzymatic, interfacial, and metabolomic analyses. ESM1 demonstrated significantly higher degradation efficiency (41.7 %) than P1 (19.4 %). Superior degradation by ESM1 was confirmed by elevated humic acid-like substances detected via fluorescence spectroscopy. Comparative enzyme assays revealed ESM1 produced substantially higher activities of lignin peroxidase (61.32 U/mL vs. 38.25 U/mL), manganese peroxidase (11.23 U/mL vs. 6.67 U/mL), and lipase (13.1 U/mL vs. 7.2 U/mL). Gas chromatography-mass spectrometry showed ESM1 treatment generated more long-chain alkanes (77.7 % vs. 66.0 %). Scanning electron microscopy indicated stronger ESM1 adhesion (adsorption rate: 56.5 % vs. 23.8 %), attributed to polysaccharide-rich extracellular substances and favorable electrostatic interactions. Metabolomic profiling identified 205 differentially expressed metabolites in ESM1, enriched in cofactor biosynthesis, phenylalanine metabolism, pyrimidine metabolism, and styrene degradation pathways, suggesting their critical role in its enhanced degradation ability. These findings provide a foundation for optimizing microbial consortia for sustainable coal conversion.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"161 ","pages":"Pages 249-260"},"PeriodicalIF":4.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145883314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of technological, chemical, biological aspects, and in-silico pharmacokinetic and docking predictions of winter savory (Satureja montana L.) essential oil 技术，化学，生物方面的调查，以及冬季风味（Satureja montana L.）精油的计算机药代动力学和对接预测

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-02-01 Epub Date: 2025-12-13 DOI: 10.1016/j.procbio.2025.12.009

Snežana Filip , Saša D. Đurović , Slavica Blagojević , Olivera Delić , Angi E. Skhvediani , Andrey V. Vasin , Darko M. Micić

{"title":"Investigation of technological, chemical, biological aspects, and in-silico pharmacokinetic and docking predictions of winter savory (Satureja montana L.) essential oil","authors":"Snežana Filip , Saša D. Đurović , Slavica Blagojević , Olivera Delić , Angi E. Skhvediani , Andrey V. Vasin , Darko M. Micić","doi":"10.1016/j.procbio.2025.12.009","DOIUrl":"10.1016/j.procbio.2025.12.009","url":null,"abstract":"<div><div>Hydrodistillation (HD 205 W and 410 W) and microwave-assisted hydrodistillation (MWHD 90–800 W) were used to prepare essential oil samples from winter savory. Isolated essential oils were analyzed to determine their chemical profiles. Carvacrol was the principal compound, followed by <em>p</em>-cymene and γ-terpinene. Biological activity was assessed using six antioxidant assays, an antimicrobial assay (6 different microbes), and a cytotoxic test (4 cell lines). The MWHD 360 W was the most potent antioxidant agent. The HD 205 W and MWHD 360 W showed the best antimicrobial activity, while the HD essential oil samples were the most potent cytotoxic agents. Molecular docking was performed for the eight most abundant compounds on three proteins: <em>E. coli</em> type I signal peptidase (PDB code 1T7D), <em>P. aeruginosa</em> arginine rhamosyltransferase (PDB code 6J7J), and <em>S. aureus</em> penicillin G acyl-Penicillin binding protein 2a (PDB code 1MWT). Carvacryl acetate and <em>trans-</em>caryophyllene provide the most promising results (the lowest binding energy). The ADMET (absorption, distribution, metabolism, excretion, and toxicity) calculations revealed low toxicity, high skin, blood-brain barrier, and CNS permeability, and high intestinal absorption.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"161 ","pages":"Pages 188-203"},"PeriodicalIF":4.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145786576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of C-phycocyanin purification by aqueous two-phase system based on a thermo-sensitive copolymer with mild critical solution temperature 基于温和临界温度的热敏共聚物的两水相体系纯化c -藻蓝蛋白的优化

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-02-01 Epub Date: 2025-11-19 DOI: 10.1016/j.procbio.2025.11.010

Shadi Azar, Alireza Habibi , Farshad Rahimpour

{"title":"Optimization of C-phycocyanin purification by aqueous two-phase system based on a thermo-sensitive copolymer with mild critical solution temperature","authors":"Shadi Azar, Alireza Habibi , Farshad Rahimpour","doi":"10.1016/j.procbio.2025.11.010","DOIUrl":"10.1016/j.procbio.2025.11.010","url":null,"abstract":"<div><div>This study focuses on the purification of phycocyanin from <em>spirulina platensis</em> (C-PC) by a novel aqueous two-phase system (ATPS) including poly(EO<sub>50</sub>-ran-PO<sub>50</sub>) (Mn ∼ 12000 Da) + phosphate salts + water. The advance of this system was the separation of C-PC in the polymer phase after reaching the equilibrium state where 77.5 % of the polymer could be recovered by heating at a temperature above 48 °C. Optimization of the process was carried out by the response surface methodology and setting pH at 6, temperature of 25 °C, phosphate salt solution content of 30 wt%, and polymer content of 28.9 wt% obtained the best condition for achievement of the highest purification fold (<em>PF</em>), purity index (<em>PI</em>) and C-PC recovery yield (<em>R</em>) equaled to 1.68, 1.06 and 95.51 %, respectively. The reusability of the recovered polymer at the three next purifications showed reasonable sustainability with the preservation of more than 90 % of the original performance. The main advantage of this ATPS for C-PC purification is a fast partitioning of C-PC into the polymer phase after 3 h, where the mild critical solution temperature of the copolymer offers a safe thermal recovery of the polymer from purified C-PC without the need to centrifugation or filtration.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"161 ","pages":"Pages 63-74"},"PeriodicalIF":4.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145570699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel and high-efficiency supplementary lighting strategy to enhance the growth of Synechococcus sp. PCC 7002 一种促进聚珠球菌pcc7002生长的新型高效辅助照明策略

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-02-01 Epub Date: 2025-12-19 DOI: 10.1016/j.procbio.2025.12.013

Tao Yu , Jie Ling , Fei Fan , Weiliang Wang , Wenmin Bai , Minxi Wan , Yuanguang Li

{"title":"A novel and high-efficiency supplementary lighting strategy to enhance the growth of Synechococcus sp. PCC 7002","authors":"Tao Yu , Jie Ling , Fei Fan , Weiliang Wang , Wenmin Bai , Minxi Wan , Yuanguang Li","doi":"10.1016/j.procbio.2025.12.013","DOIUrl":"10.1016/j.procbio.2025.12.013","url":null,"abstract":"<div><div>The objective of this study was to establish an effective night-time supplementary lighting strategy for improving the growth rate of <em>Synechococcus</em> sp. PCC 7002. Based on the indoor experimental data collected under varying light intensities, light qualities, light-dark cycle durations, and light-dark ratios, Response Surface Methodology (RSM) was employed to determine the optimal parameters for night-time illumination at different growth stages. The optimal culture conditions during the early growth stage (0–4 days) were identified as: light intensity of 140 μmol m<sup>−2</sup>·s<sup>−1</sup>, red-blue light ratio of 2.6:1, light-dark cycle duration of 110 min, light-dark ratio of 6.3:1, and inoculation age of 6 days. For the middle growth stage (5–8 days), the culture conditions that produced the highest biomass concentration were: light intensity of 173 μmol m<sup>−2</sup>·s<sup>−1</sup>, red-blue light ratio of 3.2:1, light-dark cycle duration of 116 min, light-dark ratio of 6.4:1, and inoculation age of 6 days. In the late cultivation period (9–12 days), no supplementary lighting was required. The indoor and outdoor cultivation were then compared under the different supplementary lighting strategies. The results demonstrated that the staged night-time supplementary lighting strategy achieved the highest biomass productivity under both indoor and outdoor conditions. During the initial 0–8 days of cultivation, the biomass productivity reached 0.58 g L<sup>−1</sup> d<sup>−1</sup> indoors and 0.50 g L<sup>−1</sup> d<sup>−1</sup>, outdoors, more than 42.00 % higher than other lighting strategies. The staged, RSM-optimized night-time supplementary lighting strategy significantly improve the biomass concentration of <em>Synechococcus</em> sp. PCC 7002 while also reducing the overall light energy consumption.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"161 ","pages":"Pages 225-235"},"PeriodicalIF":4.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145839655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Valorization of polyester wastes into polyhydroxyalkanoates via a one-step microbial fermentation process 通过一步微生物发酵工艺将聚酯废料转化为聚羟基烷酸酯

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-02-01 Epub Date: 2026-01-01 DOI: 10.1016/j.procbio.2025.12.019

Fernando Santos-Beneit , Sergio Bordel , Diego Martín-González , Carlos de la Fuente , Octavio García-Depraect , Tim Börner , Rosa Aragão Börner , Raúl Muñoz

{"title":"Valorization of polyester wastes into polyhydroxyalkanoates via a one-step microbial fermentation process","authors":"Fernando Santos-Beneit , Sergio Bordel , Diego Martín-González , Carlos de la Fuente , Octavio García-Depraect , Tim Börner , Rosa Aragão Börner , Raúl Muñoz","doi":"10.1016/j.procbio.2025.12.019","DOIUrl":"10.1016/j.procbio.2025.12.019","url":null,"abstract":"<div><div>Polyhydroxyalkanoates (PHAs) are promising biodegradable alternatives to petroleum-based plastics, yet their large-scale application remains hindered by high production costs. Here, a direct and cost-effective microbial strategy to upcycle polyester waste into PHAs using <em>Paracoccus denitrificans</em> was investigated. Remarkably, the bacterium metabolized 10 out of 12 tested plastic-derived monomers and efficiently accumulated intracellular PHAs, even when fed with pretreated mixed polyester waste instead of purified substrates. More than 80 % of polyester monomers supported growth, and medium optimization through nitrogen reduction boosted PHA content up to 30 % of cell dry weight. This one-step process circumvents costly separation steps, enabling the valorization of heterogeneous plastic mixtures and significantly reducing both economic and processing burdens. By transforming post-consumer plastics into high-value biopolymers, this work positions <em>P. denitrificans</em> as a versatile platform for circular bioeconomy strategies, directly supporting global sustainability agendas and advancing sustainable bioprocessing for the green industry.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"161 ","pages":"Pages 283-289"},"PeriodicalIF":4.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145924345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0