Process Biochemistry最新文献_第4页

Production of single cell protein from sugarcane molasses for feed protein and techno-economic analysis 用甘蔗糖蜜生产饲料蛋白用单细胞蛋白及其技术经济分析

IF 4 3区生物学

Process Biochemistry Pub Date : 2025-08-13 DOI: 10.1016/j.procbio.2025.08.003

Yilian Li , Jing Wu , Pengfei Wu , Chen Wang , Ruichao Huang , Jianan Zhang

{"title":"Production of single cell protein from sugarcane molasses for feed protein and techno-economic analysis","authors":"Yilian Li , Jing Wu , Pengfei Wu , Chen Wang , Ruichao Huang , Jianan Zhang","doi":"10.1016/j.procbio.2025.08.003","DOIUrl":"10.1016/j.procbio.2025.08.003","url":null,"abstract":"<div><div>The livestock industry has undergone rapid expansion in recent years, resulting in an increased demand for feed protein. The production of plant and animal proteins cannot sustainably meet the expanding demand for protein, and the development of single cell protein (SCP) as a feed protein is a promising avenue. Molasses is a cheap and nutritious by-product of sugar production from sugarbeet and sugarcane, and is one of the common substrates available to microorganisms. In this study, <em>R. glutinis</em> As 2.703 was used to produce SCP by fermentation with inexpensive carbon source molasses and nitrogen source corn steep liquor instead of glucose and yeast extract, respectively. The results revealed that when the concentration of molasses in the substrate was 61 g/L, the biomass concentration reached 21.0 g/L, and the protein, lipid, carotenoid contents in dry cell were 30.4 %, 7.8 %, and 31.7 μg/g, respectively. SuperPro Designer was used to simulate the process and carry out the economic analysis for producing SCP from 100,000 tons of molasses annually. A plant of this size could produce 32,908 tons/year of SCP at cost of 988.2 USD/ton with payback period of 6.3 years. Sensitivity analysis showed that the selling price of SCP has the greatest impact on the economics of industrial production of SCP, and that by increasing its protein and carotenoid content, its value can be increased, and further increasing its selling price is the key to achieving industrialisation. This study provides new ideas to cope with the future protein feed crisis.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 308-317"},"PeriodicalIF":4.0,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144852924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipase separation from a new source of Bacillus cereus NC7401 using a cost-effective and eco-friendly approach of surfactant/xylitol biphasic system 表面活性剂/木糖醇双相体系分离蜡样芽孢杆菌NC7401脂肪酶的研究

IF 4 3区生物学

Process Biochemistry Pub Date : 2025-08-09 DOI: 10.1016/j.procbio.2025.08.001

Kulsoom Akhter , Irsa Zaheer , Azeem Bibi , Uzma Azeem Awan , Aamir Rasheed , Zeeshan Anjum

{"title":"Lipase separation from a new source of Bacillus cereus NC7401 using a cost-effective and eco-friendly approach of surfactant/xylitol biphasic system","authors":"Kulsoom Akhter , Irsa Zaheer , Azeem Bibi , Uzma Azeem Awan , Aamir Rasheed , Zeeshan Anjum","doi":"10.1016/j.procbio.2025.08.001","DOIUrl":"10.1016/j.procbio.2025.08.001","url":null,"abstract":"<div><div>In the present study, lipase was successfully separated for the first time from <em>Bacillus cereus</em> NC7401 using a novel micellar aqueous biphasic system (ATPS) composed of xylitol and surfactants. ATPS offered eco-friendly, simple, and cost-effective enzyme separation. A fresh culture of <em>Bacillus cereus</em> NC7401 was inoculated, and enzyme production was carried out using a basal medium. After centrifugation to separate cells, the culture filtrate was used for enzyme activity assays. Three ATPS systems composed of xylitol and surfactants (Triton X-100, tween-80, and sodium dodecyl sulfate) were evaluated to identify the optimal composition. Phase diagrams were constructed to determine appropriate concentrations of surfactants and xylitol. ATPS composed of Triton X-100 (6 % w/w) and xylitol (40 % w/w) achieved a maximum purification fold of 15.4 and a yield of 96 %. Adding 5 % NaCl to the crude lipase extract raised the separation factor to 16.9. The separated lipase exhibited optimal activity at 40–50 <strong>°C</strong> and pH 7.0. Fe<sup>+ 2</sup> stimulated, while Ni<sup>+2</sup> showed an inhibitory effect on lipase partitioning as determined by titrimetric analysis. The high separation efficiency, yield, and reduced processing time of the surfactant/xylitol ATPS highlighted its potential for industrial applications in biocatalysis, pharmaceutical processing, and other enzyme-dependent processes.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 291-299"},"PeriodicalIF":4.0,"publicationDate":"2025-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144829739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Untargeted exometabolic profiling unveils natural product repertoire of the fast-growing cyanobacterium Synechococcus elongatus PCC 11801 非靶向外代谢分析揭示了快速生长的蓝细菌长聚球菌PCC 11801的天然产物库

IF 4 3区生物学

Process Biochemistry Pub Date : 2025-08-07 DOI: 10.1016/j.procbio.2025.08.002

Virmal S. Jain , Gaurav G. Bingi , Snehal D. Ganjave , Renu Pandey , Avinash Sunder , Pramod P. Wangikar

{"title":"Untargeted exometabolic profiling unveils natural product repertoire of the fast-growing cyanobacterium Synechococcus elongatus PCC 11801","authors":"Virmal S. Jain , Gaurav G. Bingi , Snehal D. Ganjave , Renu Pandey , Avinash Sunder , Pramod P. Wangikar","doi":"10.1016/j.procbio.2025.08.002","DOIUrl":"10.1016/j.procbio.2025.08.002","url":null,"abstract":"<div><div><em>Synechococcus elongatus</em> PCC 11801 is a fast-growing cyanobacterium with demonstrated potential to be employed as cell factory for photosynthetic biomanufacturing. Cyanobacteria are also a source of several natural products and bioactive compounds. We employed untargeted metabolomics of spent culture media to (i) characterize extracellular metabolite profiles associated with varying nutrient availability, and (ii) explore the natural product biosynthesis capacity of PCC 11801. We identified over 200 metabolites with high-confidence spectral matching against MS2 library, including several terpenoid secondary metabolites and bioactive compounds widely used in the cosmetic, fragrance, and pharmaceutical industries. We observed higher diversity and concentrations of such metabolites in 1X BG-11 medium, while PCC 11801 displayed extended growth phase and 6-fold higher biomass on 5X BG-11. Our findings highlight the diversity and abundance of primary and secondary metabolites produced by PCC 11801, and the potential of the cyanobacterial strain as a host for sustainable production of bioactive compounds.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 300-307"},"PeriodicalIF":4.0,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144842429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlations between characteristic flavor substances and microbial diversity in Guizhou Douchiba 贵州豆赤叶特有风味物质与微生物多样性的相关性研究

IF 4 3区生物学

Process Biochemistry Pub Date : 2025-07-30 DOI: 10.1016/j.procbio.2025.07.018

Yurou Yang , Panpan Yang , Qin Wang , Anyan Wen , Likang Qin , Haiying Zeng

{"title":"Correlations between characteristic flavor substances and microbial diversity in Guizhou Douchiba","authors":"Yurou Yang , Panpan Yang , Qin Wang , Anyan Wen , Likang Qin , Haiying Zeng","doi":"10.1016/j.procbio.2025.07.018","DOIUrl":"10.1016/j.procbio.2025.07.018","url":null,"abstract":"<div><div>Douchiba, a unique fermented food of Douchi in Guizhou, China, is well known for its rich and long-lasting flavor and dark-oily appearance. However, flavor substances and microbial community of Douchiba and the correlations between them are poorly understood. In this study, the differences in taste components, volatile flavor substances, and microbial diversity of Douchiba from seven regions and corresponding correlations were explored. Taste components in Douchiba were mainly sweet and bitter amino acids, succinic acid, and lactic acid. A total of 71 volatile compounds were identified and the main volatile flavor contributors were mainly classified into acids, pyrazines, and esters. Notably, 29 characteristic compounds were screened via OPLS-DA and VIP analysis, including tetramethylpyrazine, tartaric acid, isovaleric acid, and glutamic acid. The bacterial communities (mainly <em>Bacillus</em>) dominated over fungi. <em>Tetragenococcus</em> and <em>Aspergillus</em> were the core genera. The correlation analysis confirmed that <em>Bacillus</em> exhibited the significant correlations with pyrazines mainly generated via biotransformation and Maillard reaction pathways, whereas <em>Aspergillus</em> synergistically enhanced acidic compounds through carbohydrate metabolism. These findings provide novel insights into the microbial-driven flavor formation mechanism and a theoretical basis for screening industrial starter cultures and flavor regulation in Douchiba.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 281-290"},"PeriodicalIF":4.0,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144780460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Paenibacillus mucilaginosus-induced CO2 fixation into amorphous and crystalline carbonate minerals 粘支芽孢杆菌诱导二氧化碳固定成无定形和结晶碳酸盐矿物

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-07-22 DOI: 10.1016/j.procbio.2025.07.017

Mengdie Huang , Xuecheng Zheng , Xin He , Xiaosha Lin , Jun Ye

{"title":"Paenibacillus mucilaginosus-induced CO2 fixation into amorphous and crystalline carbonate minerals","authors":"Mengdie Huang , Xuecheng Zheng , Xin He , Xiaosha Lin , Jun Ye","doi":"10.1016/j.procbio.2025.07.017","DOIUrl":"10.1016/j.procbio.2025.07.017","url":null,"abstract":"<div><div>Biomineralization offers a potential solution to CO<sub>2</sub> fixation during oil exploitation. This study investigates the pathways of CO<sub>2</sub> conversion and crystal nucleation mechanisms induced by <em>Paenibacillus mucilaginosus ZH-28</em>. At 16 % CO<sub>2</sub> (v/v), Carbonic Anhydrase (CA) activity produced by ZH-28 reached 156.68 ng/mL, promoting mineral deposition of 6.55 mg/mL, indicating potential for in-situ carbon fixation. The study reveals that biological acids promote amorphous calcium carbonate (ACC) precipitation, while high calcium ion concentrations accelerate ACC conversion to crystalline calcite. The thermogravimetric results showed that during the gradual transformation of ACC into calcite, large numbers of free cells and organic matter around ACC were encapsulated by the calcite. Molecular dynamics simulations verified that CA catalyzes CO<sub>2</sub> to HCO<sub>3</sub><sup>-</sup> through a bicarbonate tetrahedral intermediate. The plugging potential of microbial-induced minerals was evaluated by microscopic displacement experiments and simulated core plugging experiment. Micro-morphological observations suggest that mineralization occurs via contact-mediated secondary nucleation, propagating in a zigzag pattern within 8–30 μm amorphous units. These findings provide insights into the transformation of amorphous materials into crystalline forms and offer new perspectives for CO<sub>2</sub>-microbial enhanced oil recovery (MEOR).</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 242-255"},"PeriodicalIF":3.7,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144702650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temperature fluctuations drove the interactions dynamic of keystone microbes for Chinese baijiu fermentation 温度波动驱动白酒发酵关键微生物相互作用动态

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-07-21 DOI: 10.1016/j.procbio.2025.07.016

Zijian Miao , Xinlei Wang , Wenjing Wang , Bowen Wang , Jinyuan Sun , Zexia Li , Fuping Zheng , Yuhang Zhang , Baoguo Sun

{"title":"Temperature fluctuations drove the interactions dynamic of keystone microbes for Chinese baijiu fermentation","authors":"Zijian Miao , Xinlei Wang , Wenjing Wang , Bowen Wang , Jinyuan Sun , Zexia Li , Fuping Zheng , Yuhang Zhang , Baoguo Sun","doi":"10.1016/j.procbio.2025.07.016","DOIUrl":"10.1016/j.procbio.2025.07.016","url":null,"abstract":"<div><div>Temperature is an important abiotic factor to drive the spontaneous food fermentations, however, the effects of temperature on these processes still remain unclear. In this study, we revealed the dynamics of temperature, microbiota and metabolites in the typical Chinese baijiu fermentation. Moreover, we established a specific data-driven correlation analysis model based on machine learning. We revealed 13 high-abundance and 6 low-abundance species as the keystone microbes in this fermentation, by overall considering abundance, function and frequency of fermented microorganisms. In addition, the increasing of heating rate (from 2 to 5 ℃/day) strengthened the bacterial-bacterial interactions and weakened the bacterial-fungal interactions among these keystone microbes, subsequently affecting the metabolite shifts in baijiu fermentation. Therefore, we proposed a data-driven keystone microbe’s identification framework, and revealed the effects of temperature fluctuation on the interaction and metabolism of keystone microbes. Moreover, this study would pave the way for the identification of keystone microbes within complex microbial communities, and guide the optimization of fermentation processes for improving the quality of fermented products.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 197-206"},"PeriodicalIF":3.7,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144695201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a flow microreactor with immobilized catechol-1,2-dioxygenase for the biocatalytic production of cis,cis-muconic acid 固定化儿茶酚-1,2-双加氧酶流动微反应器的研制及其生物催化生产顺式、顺式粘膜酸的研究

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-07-21 DOI: 10.1016/j.procbio.2025.07.014

Samuele Rosso , Fernando Almazán , Ismael Pellejero , Luis M. Gandía , Sabrina Dezzani , Lisa Barbieri , Gianfranco Gilardi , Francesca Valetti , Giovanna Di Nardo

{"title":"Development of a flow microreactor with immobilized catechol-1,2-dioxygenase for the biocatalytic production of cis,cis-muconic acid","authors":"Samuele Rosso , Fernando Almazán , Ismael Pellejero , Luis M. Gandía , Sabrina Dezzani , Lisa Barbieri , Gianfranco Gilardi , Francesca Valetti , Giovanna Di Nardo","doi":"10.1016/j.procbio.2025.07.014","DOIUrl":"10.1016/j.procbio.2025.07.014","url":null,"abstract":"<div><div>A low cost microreactor was developed by stable immobilization of catechol-1,2-dioxygenase for the flow biocatalysis of catechol to produce <em>cis,cis</em>-muconic acid, a direct precursor of industrially relevant adipic acid. The microreactors of polydimethylsiloxane were manufactured with different designs by straightforward, cost-effective soft lithography techniques, and were tested with different immobilization strategies focused on preserving enzyme catalytic activity and stability over time. The best immobilization strategy was covalent immobilization by amino-silanization followed by glutaraldehyde linker grafting. The biocatalytic activity of the immobilized enzyme was assessed by measuring muconic acid production through the flow of 50 µM catechol at room temperature. Catechol conversion rates of up to 95 % were achieved over a 5-hour period, and volumes of up to 180 mL were processed using only 0.32 nmol of immobilized protein, resulting in the production of 1.33 mg·L<sup>−1</sup>·h<sup>−1</sup>. This work demonstrates the feasibility of using microreactor-based flow biocatalysis for sustainable muconic acid production and highlights its promising application at industrial scale.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 232-241"},"PeriodicalIF":3.7,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144702754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enzyme supplementation as a strategy for enhancing the cellulose and hemicellulose conversion yields at high solids content of pretreated rice straw 在高固含量条件下，酶补充对提高预处理稻秆纤维素和半纤维素转化率的影响

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-07-21 DOI: 10.1016/j.procbio.2025.07.013

Giovanni H. Silva, Miguel A.D. Flores-Alarcón, Lina M. Durán, Inês C. Roberto

{"title":"Enzyme supplementation as a strategy for enhancing the cellulose and hemicellulose conversion yields at high solids content of pretreated rice straw","authors":"Giovanni H. Silva, Miguel A.D. Flores-Alarcón, Lina M. Durán, Inês C. Roberto","doi":"10.1016/j.procbio.2025.07.013","DOIUrl":"10.1016/j.procbio.2025.07.013","url":null,"abstract":"<div><div>An efficient strategy for the high-solids enzymatic hydrolysis of lignocellulosic biomass is crucial for producing a sugar-rich hydrolysate. In this sense, the effect of Viscozyme L supplementation at different loads (5 – 60 mg protein/g hemicellulose) of Cellic CTec2 on cellulose (GCY) and hemicellulose conversion yield (HCY) was evaluated, using 20 % w/v of pretreated rice straw in shake flasks. At optimum Viscozyme L load (15 mg protein/g hemicellulose), 87 and 66 % of GCY and HCY were achieved, respectively. Then, the release of sugars was further improved by using an anchor-type impeller reactor. The resulting hydrolysate, composed of (g/L): glucose (∼85), xylose (∼38), and arabinose (∼8), was finally fermented by <em>Candida guilliermondii</em> FTI 20037 yeast, showing high efficiency (96 %) and volumetric productivity (2.06 g/L/h) from glucose. Therefore, supplementation with commercially available enzymes in a non-conventional reactor improved the performance of high-solids enzymatic hydrolysis and could be a promising strategy for industrial applications.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 207-215"},"PeriodicalIF":3.7,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144695202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced nitrogen removal in single-stage sequencing batch rotating biological contactors 单级测序间歇式旋转生物接触器对氮的强化去除

IF 4 3区生物学

Process Biochemistry Pub Date : 2025-07-21 DOI: 10.1016/j.procbio.2025.07.015

Liang Cheng , Can Wang , Shining Xu , Shenliang Chen , Hui Jia , Abdallah Abdelfattah

{"title":"Enhanced nitrogen removal in single-stage sequencing batch rotating biological contactors","authors":"Liang Cheng , Can Wang , Shining Xu , Shenliang Chen , Hui Jia , Abdallah Abdelfattah","doi":"10.1016/j.procbio.2025.07.015","DOIUrl":"10.1016/j.procbio.2025.07.015","url":null,"abstract":"<div><div>The Rotating Biological Contactor (RBC), recognized for its energy efficiency, eliminates the need for aeration and sludge return in wastewater treatment. This study evaluates a Biocage-modified RBC, aimed at enhancing biomass retention and optimizing treatment efficacy. We compare continuous (C-DRBC) and sequential batch influent modes (SB-DRBC) concerning denitrification effectiveness. The continuous mode shows limited total nitrogen (TN) removal at 32.8 %, constrained by higher dissolved oxygen levels in the biofilm, which impede stable denitrification. Conversely, the SB-DRBC, operating at a hydraulic retention time of 6 h and a rotation speed of 3 rpm, substantially increases TN removal to 64.1 %. This enhancement is attributed to the development of an anoxic zone within the biofilm, conducive to denitrification. The biofilm's uptake of polyhydroxyalkanoates and glycogen metabolism by glycogen-accumulating organisms further augments nitrogen removal. Microbial analyses indicate a predominance of denitrifying bacteria such as <em>Paracoccus</em>, <em>Thauera</em>, and <em>Azoarcus</em> in the SB-DRBC mode. Reducing rotational speed fosters larger anoxic zones, thus elevating denitrification efficiency. These findings advocate the SB-DRBC approach as a formidable strategy for achieving efficient nitrogen removal and energy conservation in wastewater treatment.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 270-280"},"PeriodicalIF":4.0,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144721284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nitrogen removal characteristics and auto-aggregation capacity of the heterotrophic nitrification–aerobic denitrification bacterium, Bacillus amyloliquefaciens N8 异养硝化-好氧反硝化细菌解淀粉芽孢杆菌N8的脱氮特性及自聚集能力

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-07-19 DOI: 10.1016/j.procbio.2025.07.012

Bo Liu , Xingyuan Liu , Wenqian Zhang , Tingyu Wang , Beiping Tan , Chaoxia Ye

{"title":"Nitrogen removal characteristics and auto-aggregation capacity of the heterotrophic nitrification–aerobic denitrification bacterium, Bacillus amyloliquefaciens N8","authors":"Bo Liu , Xingyuan Liu , Wenqian Zhang , Tingyu Wang , Beiping Tan , Chaoxia Ye","doi":"10.1016/j.procbio.2025.07.012","DOIUrl":"10.1016/j.procbio.2025.07.012","url":null,"abstract":"<div><div>At present, the main factors restricting the development of high-salinity recirculating aquaculture systems are high costs and the control of inorganic nitrogen pollutants, but only a few studies have reported on salt-tolerant HN-AD strains and their denitrification mechanisms and quantification capabilities. Strain N8 was obtained from seawater biofilter and identified as <em>Bacillus amyloliquefaciens</em> based on the analysis of 16S rRNA gene sequence. Strain N8 has a good ability to remove ammonia nitrogen, nitrite and nitrate at three different concentrations (20, 50, 100 mg L<sup>−1</sup>) with little accumulation of intermediates, and the maximum removal rate of ammonia nitrogen (50 mg L<sup>−1</sup>), nitrite (100 mg L<sup>−1</sup>), and nitrate (100 mg L<sup>−1</sup>) were 96.56 %, 99.20 % and 90.20 % after 72 h incubation. Through inhibitor and nitrogen balance experiments, it was found that the first step of degradation of ammonia nitrogen by strain N8 was to oxidize it to hydroxylamine, and 72.28 % of ammonia nitrogen and 46.31 % of nitrate nitrogen were eventually converted into intracellular nitrogen. Two denitrification related genes, <em>nirK</em> and <em>nirS</em>, were successfully amplified from strain N8. The ideal conditions for growth and nitrogen removal of strain N8 were sodium succinate as the carbon source, C/N of 20, and salinity between 0 and 30 ‰. Furthermore, the highest auto-aggregation rate of the strain was above 25 % under different nitrogen sources, and the biofilm growth amount of the strain was 3.04 after 24 h culture. All results demonstrate that strain N8 has great practical application potential in nitrogen removal in high-salinity recirculating aquaculture systems.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 256-269"},"PeriodicalIF":3.7,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144702753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0