Process Biochemistry最新文献_第4页

Autocatalytic hydrothermal pretreatment for cassava bagasse depolymerization

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-02-28 DOI: 10.1016/j.procbio.2025.02.015

Marcio Ricardo Graff , Edson Antônio da Silva , Thompson Ricardo Weiser Meier , Paulo André Cremonez , Dilcemara Cristina Zenatti , Sílvio Cesar Sampaio

{"title":"Autocatalytic hydrothermal pretreatment for cassava bagasse depolymerization","authors":"Marcio Ricardo Graff , Edson Antônio da Silva , Thompson Ricardo Weiser Meier , Paulo André Cremonez , Dilcemara Cristina Zenatti , Sílvio Cesar Sampaio","doi":"10.1016/j.procbio.2025.02.015","DOIUrl":"10.1016/j.procbio.2025.02.015","url":null,"abstract":"<div><div>This study evaluated pretreatment methods for cassava bagasse (CB) degradation before biodigestion: I) using cassava processing wastewater (CW) and water, and II) using the liquid hydrolysate from the first step as a catalytic agent. Mixtures of CB (6–134 g), CW (190–318 g), and water (76–204 g), were tested to optimize CB depolymerization, measured by yield (%) and increase (g.L<sup>−1</sup>) in reducing sugars (RS). In the first pretreatment (I) the conventional hydrothermal pretreatment (HTP) was performed, the optimal solid-liquid ratio was 0.21 (m/m), using 70 g of CB, 211.34 g of CW, and 118.67 g of water at 120ºC for 75 minutes. This resulted in a 41.32 ± 1.31 % of RS yield and an increase of 0.64 ± 0.02 g.L<sup>−1</sup> RS. In the second pretreatment (II), the optimal ratio from the first step was applied, achieving a higher yield of 73.30 % RS, an increase of 1.22 g.L<sup>−1</sup> RS. Neither furfural nor 5-hydroxymethylfurfural (5-HMF) were detected in the liquid hydrolysates. The study demonstrates that autocatalytic hydrothermal pretreatment (AHP) is an efficient strategy to depolymerize CB, enhancing its digestibility and RS availability. Moreover, incorporating CW into the hydrolysis process contributes to waste valorization, promoting environmentally sustainable practices in the cassava industry.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"153 ","pages":"Pages 110-121"},"PeriodicalIF":3.7,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143681334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-performance carboxymethyl cellulose stabilized Au-Ag nanoparticles using gamma rays for management of the airborne fungus Aspergillus sydowii AGH-5 OP593090

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-02-27 DOI: 10.1016/j.procbio.2025.02.014

Rasha Mohammad Fathy , Ghadir E. Daigham , Amira Y. Mahfouz

{"title":"High-performance carboxymethyl cellulose stabilized Au-Ag nanoparticles using gamma rays for management of the airborne fungus Aspergillus sydowii AGH-5 OP593090","authors":"Rasha Mohammad Fathy , Ghadir E. Daigham , Amira Y. Mahfouz","doi":"10.1016/j.procbio.2025.02.014","DOIUrl":"10.1016/j.procbio.2025.02.014","url":null,"abstract":"<div><h3>Background</h3><div>The emergence of fungi resistant to existing antibiotics poses a challenge to scientists. Therefore, the use of nanoparticles as an alternative to antibiotics has become necessary in diseases treatment. This investigation was conducted to evaluate the antifungal effect of highly stable CMC/Au-Ag NPs against the human pathogenic fungi <em>A. sydowii</em>.</div></div><div><h3>Methods</h3><div>CMC/Au-Ag NPs were synthesized with gamma rays and characterized using “FTIR, XRD, DLS/zeta, SAED, SEM/EDX, and HR-TEM”. Antifungal potential of CMC/Au-Ag NPs against <em>A. sydowii</em> AGH-5 OP593090 was evaluated.</div></div><div><h3>Results</h3><div>The maximum nano-forming dose of gamma irradiation was 50 kGy. CMC/Au-Ag NPs were spherical mono-dispersed with an average diameter of 32.93 nm. CMC/Au-Ag NPs at 400 µL showed significant antifungal activity with an inhibition zone of 34.2 ± 1.23 mm and 86.10 % reduction of mycelia biomass. The CMC/Au-Ag NPs displayed low cytotoxicity on normal human skin cells with IC<sub>50</sub> of 800.6 ± 3.52 µg/mL. At 1000 µg/mL, CMC/Au-Ag NPs revealed anti-inflammatory activity of 71.25 % with human erythrocytes.</div></div><div><h3>Conclusion</h3><div>CMC/Au-Ag NPs exhibited considerable antifungal activity against <em>A. sydowii</em>, suggesting potential usage in medical fields. The novelty of this study is the use of gamma irradiation in low-dose to synthesize CMC/Au-Ag bimetallic nanoparticles and their first use against the human pathogenic fungus, <em>A. sydowii.</em></div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"152 ","pages":"Pages 58-74"},"PeriodicalIF":3.7,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143549768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional evaluation of laminarin butyl esters: Antioxidant, skin-whitening, and anti-wrinkle properties

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-02-26 DOI: 10.1016/j.procbio.2025.02.012

Kit-Leong Cheong , Keying Liu , Suresh Veeraperumal , Gowsika Jaikumar , Sathuvan Malairaj , Xiaofei Liu , Xuejing Jia , Peiying Zheng , Hengyi Jiang , Ruixu Cai , Saiyi Zhong , Udayakumar Veerabagu

{"title":"Functional evaluation of laminarin butyl esters: Antioxidant, skin-whitening, and anti-wrinkle properties","authors":"Kit-Leong Cheong , Keying Liu , Suresh Veeraperumal , Gowsika Jaikumar , Sathuvan Malairaj , Xiaofei Liu , Xuejing Jia , Peiying Zheng , Hengyi Jiang , Ruixu Cai , Saiyi Zhong , Udayakumar Veerabagu","doi":"10.1016/j.procbio.2025.02.012","DOIUrl":"10.1016/j.procbio.2025.02.012","url":null,"abstract":"<div><div>Laminarin, a polysaccharide derived from brown algae, has gained attention for its potential biological activities. However, its application in cosmetic formulations is limited due to its functional properties. To enhance its bioavailability and performance, laminarin was modified through esterification to form laminarin butyl esters (LBE). This study aimed to evaluate the antioxidant, skin-whitening, and anti-wrinkle properties of LBE and assess its potential as a novel ingredient in skincare products. Antioxidant activity was evaluated using in vitro assays, including DPPH and ABTS radical scavenging, both of which demonstrated significant free radical neutralizing capacity. Skin-whitening and anti-wrinkle activities were assessed by measuring the inhibition of tyrosinase and elastase. The results indicated that LBE exhibited significantly higher inhibitory effects than native laminarin, with tyrosinase inhibition reaching 26.7 % (compared to 4.7 % for laminarin) and elastase inhibition at 45.8 % (versus 18.5 % for laminarin). Additionally, LBE showed stronger anti-glycation activity, as it effectively inhibited Amadori products, dicarbonyl compounds, and advanced glycation end-products. These findings provide valuable insights into the modification of laminarin and its potential application in cosmetics, offering a foundation for the development of more effective, bioactive ingredients in the beauty industry.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"152 ","pages":"Pages 29-37"},"PeriodicalIF":3.7,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143520308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of bubble size on microbial community in intermittently-aerated activated sludge process in wastewater treatment

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-02-26 DOI: 10.1016/j.procbio.2025.02.011

Tetsuji Okamoto , Fumitake Takahashi , Mitsuhiko Koyama , Kiyohiko Nakasaki

{"title":"Effect of bubble size on microbial community in intermittently-aerated activated sludge process in wastewater treatment","authors":"Tetsuji Okamoto , Fumitake Takahashi , Mitsuhiko Koyama , Kiyohiko Nakasaki","doi":"10.1016/j.procbio.2025.02.011","DOIUrl":"10.1016/j.procbio.2025.02.011","url":null,"abstract":"<div><div>Although finer aeration bubbles improve the oxygen dissolution efficiency and activated sludge treatment performance, the effects of the changes in bubble size on the microbial community in activated sludge during intermittent aeration are often unknown. This study investigated the effect of aeration bubble size on the total microbial community, including prokaryotes (bacteria) and eukaryotes, during wastewater treatment at a food-processing plant with intermittent aeration in a single-tank reactor. Two types of diffusers were employed, showing distinctly different bubble size distributions, as confirmed by the Sauter mean diameter and bubble diameter histograms. Although the wastewater was well treated regardless of the bubble size, the microorganisms involved in the treatment process varied widely in terms of prokaryotes and eukaryotes. In prokaryotes, the genus <em>Thiothrix</em> characteristically appeared in the fine-bubble reactor, whereas the genus <em>Kouleothrix</em> appeared in the large-bubble reactor. In eukaryotes, osmotrophs of the phylum <em>Cryptomycota</em>, a fungus, were found to be dominant in the fine-bubble reactors, whereas phagotrophs of the genera <em>Arcella</em>, <em>Euglypha</em>, and <em>Parachela</em> were found to be dominant in the large-bubble reactors. The anaerobic time per day (dissolved oxygen concentration of less than 0.1 mg/L) differed depending on the bubble size, and this was thought to be reflected in the differences in the microbial communities.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"152 ","pages":"Pages 38-46"},"PeriodicalIF":3.7,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143534684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of dynamic down-regulation systems in Clostridium tyrobutyricum for butyrate production

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-02-26 DOI: 10.1016/j.procbio.2025.02.013

Lingkai Jin, Haoran Lin, Xingjie Ma, Rui Hong, Weijian Ding, Jin Huang

{"title":"Construction of dynamic down-regulation systems in Clostridium tyrobutyricum for butyrate production","authors":"Lingkai Jin, Haoran Lin, Xingjie Ma, Rui Hong, Weijian Ding, Jin Huang","doi":"10.1016/j.procbio.2025.02.013","DOIUrl":"10.1016/j.procbio.2025.02.013","url":null,"abstract":"<div><div>The acetate biosynthesis pathway is an important component of central carbon metabolism, providing adenosine triphosphate (ATP) and acetate for cell growth. However, enhanced acetate production tends to be toxic to the cell and inhibits butyrate production by <em>Clostridium tyrobutyricum</em>. In this study, we evaluated the expression strength of 22 endogenous promoters, whose expression levels ranged from 4.4 % to 121 % of the strong promoter P<sub><em>thl</em></sub>. By integrating the endogenous promoters with 8 degradation tag mutants, we obtained the optimal combination of P<sub><em>sigK</em></sub>-LAA+ 4 after evaluating the constructed dynamic down-regulation systems. P<sub><em>sigK</em></sub>-LAA+ 4 was used for the dynamic downregulation of the acetate branch pathway to improve butyrate production. Although the titers, yields and selectivity of butyrate production were not improved significantly, productivities of P<sub><em>sigK</em></sub>-<em>pta-ack</em>-LAA+ 4 and P<sub><em>sigK</em></sub>-<em>pta</em>-LAA+ 4-<em>ack</em> were increased by 84 % and 121 %, due to the shortened fermentation period and higher tolerance to acetate. Additionally, downregulating the acetate branch pathway in the logarithmic growth phase and overexpressing key enzymes involved in the acetate branch pathway in the stationary phase could effectively mitigate acetate toxicity and improve butyrate production. To our knowledge, this study is the first to report the dynamic downregulation systems in <em>C. tyrobutyricum</em>, providing potential regulation strategies for biofuel production in the future<em>.</em></div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"152 ","pages":"Pages 47-57"},"PeriodicalIF":3.7,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143549859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lactic acid bacteria: Nature, characterization, mode of action, products and applications

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-02-21 DOI: 10.1016/j.procbio.2025.02.010

Patrick Othuke Akpoghelie , Great Iruoghene Edo , Ali B.M. Ali , Emad Yousif , Khalid Zainulabdeen , Joseph Oghenewogaga Owheruo , Endurance Fegor Isoje , Ufuoma Augustina Igbuku , Arthur Efeoghene Athan Essaghah , Raghda S. Makia , Dina S. Ahmed , Huzaifa Umar , Ahmed A. Alamiery

{"title":"Lactic acid bacteria: Nature, characterization, mode of action, products and applications","authors":"Patrick Othuke Akpoghelie , Great Iruoghene Edo , Ali B.M. Ali , Emad Yousif , Khalid Zainulabdeen , Joseph Oghenewogaga Owheruo , Endurance Fegor Isoje , Ufuoma Augustina Igbuku , Arthur Efeoghene Athan Essaghah , Raghda S. Makia , Dina S. Ahmed , Huzaifa Umar , Ahmed A. Alamiery","doi":"10.1016/j.procbio.2025.02.010","DOIUrl":"10.1016/j.procbio.2025.02.010","url":null,"abstract":"<div><div>Lactic acid bacteria (LAB) are a type of non-spore-forming, non-respiring, Gram-positive cocci or rods primarily responsible for producing lactic acid through the fermentation of carbohydrates. Homofermentative and heterofermentative LAB are the two main types that are known. The majority of these groups' members have been eaten for thousands of years, are considered “Generally Regarded As Safe” organisms, and do not offer any risks to human health apart from the pathogenic species. Due to their extensive use as starter cultures for fermentation in food production, which preserves and improves the flavour of a variety of dairy, meat, vegetable, and cereal products, LAB have a substantial market impact. Their market value is multibillion dollars, and the market for LAB as functional food ingredients with potential health benefits has also increased due to the growing interest in probiotics. There are numerous uses for LAB, including the food and dairy sectors, as probiotics to enhance human and animal health, as biofertilizers, and in a variety of industries as a source of exoplysaccharide. <em>Aerococcus, Carnobacterium, Enterococcus, Lactobacillus, Lactococcus, Leuconostoc, Streptococcus, Tetragenococcus, Vagococcus,</em> and <em>Weissella</em> are among the numerous genera that make up the group. The application of lactic acid bacteria in the food, agricultural, and human health industries should be further guided by research on the metabolic pathways and traits of these bacteria. The study of LAB and their functional elements is still a developing field of research that has not yet reached its full potential, despite recent advancements.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"152 ","pages":"Pages 1-28"},"PeriodicalIF":3.7,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143480669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced off-flavor compounds biotransformation by microbial interaction between Saccharomyces cerevisiae and Lactobacillus plantarum assisted by physical excitor and yeast quorum sensing

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-02-18 DOI: 10.1016/j.procbio.2025.02.008

Xiaohan Tan , Yuanrong Zheng , Changyu Zhou , Daodong Pan , Jinxuan Cao , Dong Yang , Anderson S. Sant'Ana , Hao Zhang , Qiang Xia

{"title":"Enhanced off-flavor compounds biotransformation by microbial interaction between Saccharomyces cerevisiae and Lactobacillus plantarum assisted by physical excitor and yeast quorum sensing","authors":"Xiaohan Tan , Yuanrong Zheng , Changyu Zhou , Daodong Pan , Jinxuan Cao , Dong Yang , Anderson S. Sant'Ana , Hao Zhang , Qiang Xia","doi":"10.1016/j.procbio.2025.02.008","DOIUrl":"10.1016/j.procbio.2025.02.008","url":null,"abstract":"<div><div>This study explored the mutualistic effects of yeast and <em>Lactobacillus</em> co-culture fermentation on degrading off-flavor compounds in duck liver (DL) like trimethylamine (TMA), dimethylamine (DMA), and histamine (HA). Ultrasonication (US) stimuli were applied to adjust microbial interactions and assess their impact on off-flavor metabolism. Degradation rates of TMA, DMA, and HA in single-strain fermentations reached 33 %, 24 %, and 60 %, respectively. In contrast, co-culture fermentation improved these rates to 39 %, 39.7 %, and 75 %. After US pretreatment (40 kHz, 400 W, 30 min), degradation rates further increased to 56 %, 54.7 %, and 90 %, respectively. Non-targeted analysis revealed that co-culture fermentation achieved the highest off-flavor removal, as evidenced by increased esters and decreased aldehydes. Key metabolites, including phenylacetaldehyde, phenylethanol, and D-limonene, were linked to fermentation patterns. US also elevated phenylethanol levels, a yeast quorum sensing (QS) signaling molecule, suggesting that QS-mediated interactions influenced off-flavor compound degradation. Enhanced yeast QS signaling molecules (phenylethanol, tryptophan, tyrosol) contributed to further off-flavor metabolism in US-stimulated fermentation, improving TMA degradation by 18 %-53 %, DMA by 16 %-30 %, and HA by 17 %-53 %. These findings demonstrate that US enhances the biodegradation of fishy substances by modulating yeast-Lactobacillus interactions, offering new insights into biotransformation processes.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"151 ","pages":"Pages 152-166"},"PeriodicalIF":3.7,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143465370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bridging seaweed-derived polysaccharides and gut health: An integrated perspective on intestinal morphology, microbiota, and immunity

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-02-18 DOI: 10.1016/j.procbio.2025.02.009

Jiang Zhan , Kai Liu , Huahua Yu , Ronge Xing , Song Liu , Xiaolin Chen

{"title":"Bridging seaweed-derived polysaccharides and gut health: An integrated perspective on intestinal morphology, microbiota, and immunity","authors":"Jiang Zhan , Kai Liu , Huahua Yu , Ronge Xing , Song Liu , Xiaolin Chen","doi":"10.1016/j.procbio.2025.02.009","DOIUrl":"10.1016/j.procbio.2025.02.009","url":null,"abstract":"<div><div><em>Enteromorpha prolifera</em>, a widely distributed marine alga in the Yellow Seas, has caused severe environmental and economic problems due to its bloom-induced green tides. Consequently, the efficient development and utilization of <em>Enteromorpha prolifera</em> hold significant importance. Recent studies have shown that <em>Enteromorpha prolifera</em> is abundant in bioactive substances, especially polysaccharides, which have been proven to possess antioxidant, anticancer, and anti-inflammatory activities. In recent years, more and more studies have shown that seaweed polysaccharides show great potential in improving intestinal health, and the intestinal microbiota has emerged as a promising target for seaweed polysaccharides to exert their efficacy. However, a comprehensive summary of the interactions between <em>Enteromorpha prolifera</em> polysaccharides (EP) and the gut remained limited. This review aimed to fill this gap by summarizing the latest studies of EP on gut health and their mechanisms from maintaining the integrity of intestinal structure and morphology, regulating the diversity and abundance of the intestinal microbiota and modulating intestinal immune response. By systematically synthesizing existing research, this review provided a robust theoretical foundation for the development of EP as a functional food and therapeutic agent, thereby broadening its potential applications in the health domain.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"151 ","pages":"Pages 146-151"},"PeriodicalIF":3.7,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143465371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation and identification of a novel antioxidative peptide from fermented protein hydrolysate of chicken (Gallus gallus domesticus) meat

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-02-17 DOI: 10.1016/j.procbio.2025.02.006

Neelu Suresh Babu , P.V. Suresh , Tanaji G. Kudre

{"title":"Preparation and identification of a novel antioxidative peptide from fermented protein hydrolysate of chicken (Gallus gallus domesticus) meat","authors":"Neelu Suresh Babu , P.V. Suresh , Tanaji G. Kudre","doi":"10.1016/j.procbio.2025.02.006","DOIUrl":"10.1016/j.procbio.2025.02.006","url":null,"abstract":"<div><div>Present investigation deals with the preparation, purification, and identification of antioxidant peptides from fermented chicken (<em>Gallus gallus</em> domesticus) meat protein hydrolysate (CMPH). Antioxidant CMPH was produced by <em>Pediococcus pentosaceus</em> fermentation using 45 % chicken meat concentration, 2 % dextrose, and 48 h of fermentation time. Antioxidant peptides were separated from CMPH by employing ultrafiltration (3 kDa MWCO), Sephadex G-15 gel filtration chromatography, and RP-HPLC, respectively. Ultrafiltration (3 kDa MWCO) revealed that CMPH-UF-1 (MW < 3 kDa) exhibited significantly higher ferric-reducing antioxidant power (FRAP) (22.70 µM TE/mg), DPPH (30.77 µM TE/mg), and ABTS radical scavenging activity (39.41 µM TE/mg) and Fe<sup>2+</sup> chelating activity (21.45 µM EDTA/mg) than CMPH-UF-2 (<em>p</em> < 0.05). Six fractions of antioxidant peptides were separated from CMPH-UF-1 by Sephadex G-15 chromatography. Among these, CMPH-GF-5 peptide fraction unveiled significantly higher antioxidant activities with FRAP (38.21 µM TE/mg), DPPH radical scavenging activity (55.91 µM TE/mg), and ABTS radical scavenging activity (68.41 µM TE/mg) and Fe<sup>2+</sup> chelating activity (36.96 µM EDTA/mg) (<em>p</em> < 0.05). Subsequent RP-HPLC purification of CMPH-GF-5 produced eleven fractions, among which CMPH-RPH-8 showed superior antioxidant activities with FRAP (65.06 µM TE/mg), DPPH radical scavenging activity (110.2 µM TE/mg), and ABTS radical scavenging activity (94.07 µM TE/mg) and Fe<sup>2+</sup> chelating activity (56.19 µM EDTA/mg). LC-MS/MS analysis identified a novel peptide in CMPH-RPH-8 (857.898 Da) with the sequence Leu-Gly-Gln-Glu-Ser-Leu-Ser-Pro-Asp, which has not been previously reported. Identified peptide was synthesized and verified the antioxidant activities. Therefore, the purified antioxidant peptide from CMPH is novel and can serve as a promising antioxidant in functional foods and nutraceutical products.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"151 ","pages":"Pages 167-176"},"PeriodicalIF":3.7,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143474225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating the storage stability of a Plasmodium vivax circumsporozoite protein vaccine candidate

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-02-13 DOI: 10.1016/j.procbio.2025.02.003

Janaína Tenorio Novais , Rodolfo Ferreira Marques , Alba Marina Gimenez , Irene Silva Soares

{"title":"Evaluating the storage stability of a Plasmodium vivax circumsporozoite protein vaccine candidate","authors":"Janaína Tenorio Novais , Rodolfo Ferreira Marques , Alba Marina Gimenez , Irene Silva Soares","doi":"10.1016/j.procbio.2025.02.003","DOIUrl":"10.1016/j.procbio.2025.02.003","url":null,"abstract":"<div><div>Malaria is a serious public health problem, with approximately 263 million cases recorded worldwide in 2023. The prevention of this disease and the development of vaccines against it remain critical issues to resolve. However, the ability of newly developed formulations to remain stable and biologically active under storage is a challenge to overcome but highly relevant, considering the places where such vaccines are needed. In this study, we evaluated the six-month storage stability of yPvCSP-All epitopes, a <em>Plasmodium vivax</em> malaria vaccine candidate, comprising a recombinant protein expressed in the yeast <em>Pichia pastoris</em>. The stability of the liquid and lyophilized formulations after storage at three different temperatures was determined using SDS-PAGE, immunoblotting, circular dichroism, SEC, RP-HPLC, and immunization assays. In liquid formulation, only the recombinant protein stored at –20°C remained stable for 180 days. By contrast, the lyophilized formulations showed no signs of degradation for six months when stored at –20°C and 5°C, and they all induced high antibody titers. These results demonstrate that the vaccine formulations can remain stable under some of the tested conditions, which is promising considering the difficulty of maintaining the cold chain in regions where malaria is endemic.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"151 ","pages":"Pages 126-136"},"PeriodicalIF":3.7,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143436751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0