Qian Li , Xianhao Lu , Keping Zhang , Xianwang Kong , Lichao Lu , Jianmeng Chen , Dongzhi Chen
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引用次数: 0
Abstract
Two-phase partitioning bioreactor (TPPB) has been developed as a promising approach to improve the biological purification of hydrophobic waste-gas. However, the biodegradation efficiency of highly hydrophobic pollutants remains limited. Herein, static magnetic field (SMF) was innovatively introduced to enhance the biodegradation of the model pollutant n-hexane and the underlying mechanism was revealed. Results showed that the influence of SMF on different n-hexane degrading bacteria varied significantly, and SMF cast the most pronounced enhancement on Pseudomonas HY-4 under the optimized intensity of 20 mT. The removal efficiency of n-hexane with an inlet concentration of 540·mg m−3 using the SMF enhanced TPPB reactor reached 93 % within 5 days, surpassing the unenhanced bioreactor (82 %). And the SMF enhanced reactor retained the original shock load resistance. Mechanism analysis revealed that, under the action of SMF, the microbial cell surface hydrophobicity was sustainably improved, thereby improving the mass transfer of n-hexane diffusing from the liquid to the microbial phase. The electron transport chain activity in the SMF-enhanced TPPB was enhanced by 10.3 %. Furthermore, the abundance of degrading bacteria beneficial to n-hexane degradation was greatly enriched. This work may lay a foundation for the application of SMF in enhancing the biodegradation of hydrophobic gaseous pollutants.
期刊介绍:
Process Biochemistry is an application-orientated research journal devoted to reporting advances with originality and novelty, in the science and technology of the processes involving bioactive molecules and living organisms. These processes concern the production of useful metabolites or materials, or the removal of toxic compounds using tools and methods of current biology and engineering. Its main areas of interest include novel bioprocesses and enabling technologies (such as nanobiotechnology, tissue engineering, directed evolution, metabolic engineering, systems biology, and synthetic biology) applicable in food (nutraceutical), healthcare (medical, pharmaceutical, cosmetic), energy (biofuels), environmental, and biorefinery industries and their underlying biological and engineering principles.