Static magnetic field enhances the purification of gaseous n-hexane in airlift two-phase partitioning bioreactor

IF 4 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Qian Li , Xianhao Lu , Keping Zhang , Xianwang Kong , Lichao Lu , Jianmeng Chen , Dongzhi Chen
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引用次数: 0

Abstract

Two-phase partitioning bioreactor (TPPB) has been developed as a promising approach to improve the biological purification of hydrophobic waste-gas. However, the biodegradation efficiency of highly hydrophobic pollutants remains limited. Herein, static magnetic field (SMF) was innovatively introduced to enhance the biodegradation of the model pollutant n-hexane and the underlying mechanism was revealed. Results showed that the influence of SMF on different n-hexane degrading bacteria varied significantly, and SMF cast the most pronounced enhancement on Pseudomonas HY-4 under the optimized intensity of 20 mT. The removal efficiency of n-hexane with an inlet concentration of 540·mg m−3 using the SMF enhanced TPPB reactor reached 93 % within 5 days, surpassing the unenhanced bioreactor (82 %). And the SMF enhanced reactor retained the original shock load resistance. Mechanism analysis revealed that, under the action of SMF, the microbial cell surface hydrophobicity was sustainably improved, thereby improving the mass transfer of n-hexane diffusing from the liquid to the microbial phase. The electron transport chain activity in the SMF-enhanced TPPB was enhanced by 10.3 %. Furthermore, the abundance of degrading bacteria beneficial to n-hexane degradation was greatly enriched. This work may lay a foundation for the application of SMF in enhancing the biodegradation of hydrophobic gaseous pollutants.
静磁场增强了气升式两相分配生物反应器中气态正己烷的净化效果
两相分配生物反应器(TPPB)是一种很有前途的改善疏水废气生物净化的方法。然而,高疏水性污染物的生物降解效率仍然有限。本文创新性地引入了静磁场(SMF)来增强模型污染物正己烷的生物降解,并揭示了其潜在的机制。结果表明,SMF对不同正己烷降解菌的影响差异显著,在优化强度为20 mT时,SMF对假单胞菌y -4的强化效果最为显著。SMF强化TPPB反应器在进口浓度为540·mg m−3时,5 d内对正己烷的去除率达到93 %,超过未强化的生物反应器(82 %)。SMF增强型反应器保持了原有的抗冲击负荷能力。机理分析表明,在SMF的作用下,微生物细胞表面疏水性持续提高,从而促进了正己烷从液体向微生物相扩散的传质。smf增强的TPPB的电子传递链活性提高了10.3 %。此外,有利于正己烷降解的降解菌的丰度也大大增加。本研究为SMF在提高疏水气体污染物生物降解方面的应用奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Process Biochemistry
Process Biochemistry 生物-工程:化工
CiteScore
8.30
自引率
4.50%
发文量
374
审稿时长
53 days
期刊介绍: Process Biochemistry is an application-orientated research journal devoted to reporting advances with originality and novelty, in the science and technology of the processes involving bioactive molecules and living organisms. These processes concern the production of useful metabolites or materials, or the removal of toxic compounds using tools and methods of current biology and engineering. Its main areas of interest include novel bioprocesses and enabling technologies (such as nanobiotechnology, tissue engineering, directed evolution, metabolic engineering, systems biology, and synthetic biology) applicable in food (nutraceutical), healthcare (medical, pharmaceutical, cosmetic), energy (biofuels), environmental, and biorefinery industries and their underlying biological and engineering principles.
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