Process Biochemistry最新文献_第6页

Metabolic engineering Corynebacterium glutamicum for production of γ-aminobutyric acid by glutamate decarboxylase active at near-neutral pH and displaying at cell surface 代谢工程谷氨酸棒状杆菌利用谷氨酸脱羧酶生产γ-氨基丁酸，该酶在接近中性的pH值下具有活性，并在细胞表面显示

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-07-04 DOI: 10.1016/j.procbio.2025.07.002

Haoqian Li , Hao Yang , Feng Shi

{"title":"Metabolic engineering Corynebacterium glutamicum for production of γ-aminobutyric acid by glutamate decarboxylase active at near-neutral pH and displaying at cell surface","authors":"Haoqian Li , Hao Yang , Feng Shi","doi":"10.1016/j.procbio.2025.07.002","DOIUrl":"10.1016/j.procbio.2025.07.002","url":null,"abstract":"<div><div>γ-Aminobutyric acid (GABA) is widely applied in pharmaceuticals, foods and feeds. <em>Corynebacterium glutamicum</em> that expresses exogenous glutamate decarboxylase (GAD) gene <em>gad</em> can produce GABA from glucose using self-produced L-glutamate. However, the incongruity between optimal pH for cell growth (7.0–7.5) and GAD (4.0–5.0) severely restricts the production of GABA. In this study, several GADs active at near-neutral pH were separately expressed in <em>C. glutamicum</em> by plasmid<em>, Ls</em>GAD derived from <em>Lactobacillus senmaizukei</em> performed better and generated 10.9 g/L GABA. Subsequently, to perform the GAD reaction at the more acidic extracellular environment, <em>Ls</em>GAD was displayed on cell surface by several anchoring motifs, and displaying by PorH and NCgl1307 motifs produced 9.9 g/L and 1.3 g/L GABA, respectively. To further improve GABA production, the metabolic pathways were modified and accompanied by integrating several <em>gad</em> genes in the chromosome, the best strain GSL-6 could produce 15.6 g/L GABA. Finally, the surface display plasmid of <em>Ls</em>GAD was introduced into the chromosomally modified strain GSL-6 to catalyze GAD reaction both intracellularly and extracellularly, and 25.3 g/L and 42.3 g/L GABA was finally produced by shake flask and fed-batch fermentation, respectively. Thereby, this synergistic strategy is beneficial for GABA production in <em>C. glutamicum</em>.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 75-83"},"PeriodicalIF":3.7,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144580830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Properties of Leuconostoc lactis isolated from traditional fermented rice beverages as a starter 从传统发酵米酒中分离的乳酸菌的特性研究

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-07-04 DOI: 10.1016/j.procbio.2025.06.019

Hikaru Ogura, Nanase Okamoto, Ayaka Nakamura, Hajime Takahashi, Riko Kamata, Takashi Kuda

{"title":"Properties of Leuconostoc lactis isolated from traditional fermented rice beverages as a starter","authors":"Hikaru Ogura, Nanase Okamoto, Ayaka Nakamura, Hajime Takahashi, Riko Kamata, Takashi Kuda","doi":"10.1016/j.procbio.2025.06.019","DOIUrl":"10.1016/j.procbio.2025.06.019","url":null,"abstract":"<div><div>The health benefits of traditional fermented rice beverages have attracted considerable attention. “Miki” from the Amami Ohshima island is made from spontaneously fermented rice saccharified with sweet potato <em>β</em>-amylase. <em>Leuconostoc lactis</em> is the dominant species isolated from Miki. In this study, we aimed to clarify the role of indigenous <em>Ln. lactis</em> in fermented rice beverages by determining the antimicrobial activity, exopolysaccharide (EPS) production capacity, and Miki fermentation properties. Among the isolates, <em>Ln. lactis</em> Miki-A3 showed superior anti-listerial activity in the agar overlay method and EPS production with antioxidant capacity (O<sub>2</sub><sup>-</sup> radical scavenging and Fe-reducing power) compared with those of the type strain. The antibiotic resistance and related genes of Miki-A3 were similar to those of the type strain. The positions and orders of EPS-related genes differed between the two strains. In the Miki fermentation model, Miki-A3 fermented well at 30 ℃ and decreased <em>Listeria monocytogenes</em> (Lm) count from 5 log to below the detection limit (< 3.3 log CFU/mL) in 24 h. In the fermented Miki stored at 10 ℃, the Lm count immediately decreased. These results indicate that Miki-A3 is a promising starter for rice-fermented beverages in terms of health functionality and hygiene.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 84-92"},"PeriodicalIF":3.7,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144580829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Water chestnut shell as a novel substrate for biosurfactant production: Enhanced oil recovery and soil decontamination 荸荠壳作为生产生物表面活性剂的新型底物：提高原油采收率和土壤净化

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-07-03 DOI: 10.1016/j.procbio.2025.07.001

Varsha Tripathi , Vivek Kumar Gaur , Natesan Manickam

{"title":"Water chestnut shell as a novel substrate for biosurfactant production: Enhanced oil recovery and soil decontamination","authors":"Varsha Tripathi , Vivek Kumar Gaur , Natesan Manickam","doi":"10.1016/j.procbio.2025.07.001","DOIUrl":"10.1016/j.procbio.2025.07.001","url":null,"abstract":"<div><div>In the realm of green chemistry, biosurfactants derived from fruit and vegetable wastes have garnered substantial interest due to their environmentally friendly applications. This study investigates the utilization of water chestnut shell powder (WCSP) as a novel substrate for biosurfactant production from <em>Pseudomonas aeruginosa</em> IITR110 and <em>Bacillus velezensis</em> IITR111. The extracted biosurfactants were identified as rhamnolipid and lipopeptide, with a yield of 1.18 g/L and 0.99 g/L, respectively. Their emulsification activities against crude oil demonstrated an E<sub>24</sub> value of 60.5 ± 1.8 % with rhamnolipid and 64.9 ± 1.4 % with lipopeptide. These biosurfactants exhibited robust stability across a wide range of pH 2.0–13.0, temperatures 4–120 ºC, and salinity levels 1–20 %, NaCl. The biosurfactants were employed in soil washing assay in which biosurfactants from IITR110 and IITR111 resulted in crude oil removal rates of 52.12 % and 58.03 %, respectively, outperforming the synthetic surfactant tween 20 (50.71 %). The sand pack column experiments revealed a substantially enhanced oil recovery percentage of 36 % for rhamnolipid and 43 % for lipopeptide. The novelty and significance of this study lie in the innovative use of water chestnut shell powder as an alternative substrate for biosurfactant production, thus presenting a sustainable and previously untapped approach.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 65-74"},"PeriodicalIF":3.7,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144549426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eco-friendly films from lemon peel pectin including essential oils for the sustainable tomato preservation 由柠檬皮果胶制成的环保薄膜，包括可持续保存番茄的精油

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-27 DOI: 10.1016/j.procbio.2025.06.018

Sirine Ben Dlala , Zeineb Mzoughi , Mariem Itaimi Dammak , Khaoula Khwaldia , Didier Le Cerf , Hamdi Ben Halima , Nicole Jaffrezic-Renault , Hafsa Korri-Youssoufi , Hatem Majdoub

{"title":"Eco-friendly films from lemon peel pectin including essential oils for the sustainable tomato preservation","authors":"Sirine Ben Dlala , Zeineb Mzoughi , Mariem Itaimi Dammak , Khaoula Khwaldia , Didier Le Cerf , Hamdi Ben Halima , Nicole Jaffrezic-Renault , Hafsa Korri-Youssoufi , Hatem Majdoub","doi":"10.1016/j.procbio.2025.06.018","DOIUrl":"10.1016/j.procbio.2025.06.018","url":null,"abstract":"<div><div>Several fruits and vegetables deteriorate within a few days, which poses a major challenge for their preservation and commercialization. To address this issue, this study reports on developing eco-friendly and safe films based on pectin extracted from lemon peel, enriched with essential oils, for a sustainable preservation of tomatoes. Pectin was extracted from <em>Citrus limon</em> peels using an innovative high-pressure treatment, achieving higher yields than conventional methods. The extracted pectin was characterized using advanced analytical techniques, FT-IR, Nuclear Magnetic Resonance (NMR), Size Exclusion Chromatography (SEC), and Gas Chromatography-Mass Spectrometry (GC-MS). Additionally, essential oils extracted from lemon and laurel were characterized by GC-MS to identify their active components. Pectin-chitosan-based films, with or without laurel and lemon essential oils, were prepared and evaluated for their mechanical properties, antimicrobial activity, moisture resistance, solubility, and biodegradability. These films were then tested for tomato preservation, demonstrating a significant extension of their shelf life beyond one month. Acting as a protective second skin, these films extend the freshness of fruits and vegetables by preventing microbial growth and reducing moisture loss. The results highlight the potential of these safe and eco-friendly films as a promising alternative to conventional plastic films.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 56-64"},"PeriodicalIF":3.7,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144535535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of novel dead-end filtration strategy for isolation of pullulan broth 蒲兰兰肉汤分离的新型终端过滤策略的开发

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-25 DOI: 10.1016/j.procbio.2025.06.016

Linyu Lu , Yiwen Li , Qingli Guan , Tingbin Zhao , Weifeng Cao , Changsheng Qiao

{"title":"Development of novel dead-end filtration strategy for isolation of pullulan broth","authors":"Linyu Lu , Yiwen Li , Qingli Guan , Tingbin Zhao , Weifeng Cao , Changsheng Qiao","doi":"10.1016/j.procbio.2025.06.016","DOIUrl":"10.1016/j.procbio.2025.06.016","url":null,"abstract":"<div><div>Pullulan, a natural extracellular polysaccharide, is widely used in food and related industries, cosmetics, and pharmaceuticals. The high viscosity of its fermentation broth poses significant challenges for efficient separation, making the process both difficult and time-consuming. In this study, cross-flow membrane filtration was first applied to separate a two-fold diluted broth (feed solution), where membranes with pore sizes ranging from 150 kDa to 0.8 μm rejected over 99 % of cells and approximately 50 % of pullulan. This phenomenon was attributed to higher shear forces increasing pullulan particle size in solution, thereby reducing its recovery rate. To address this limitation, a dead-end filtration strategy was developed using a 1–3 μm filtration medium with 1 % filter aid. During concentration-mode filtration, fouling mechanisms in the first stage showed indistinct differences between standard and intermediate blocking, while the second stage exhibited unclear distinctions between complete and intermediate blocking. A favorable membrane regeneration strategy was identified: sequential washing with fresh water followed by detergent powder. This method achieved 99.54 % biomass removal and a pullulan recovery rate of 90.67 %. Overall, the dead-end filtration strategy presents a novel approach for separating high-viscosity biopolymers, offering both efficiency and practicality for industrial applications.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 359-368"},"PeriodicalIF":3.7,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144490150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation, characterization and evaluation properties of a high-methoxyl pectin-iron (Ⅲ) from Actinidia arguta fruits 软枣猕猴桃果实高甲氧基果胶铁的制备、表征及性能评价（Ⅲ）

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-23 DOI: 10.1016/j.procbio.2025.06.013

Tienan Wang , Xinning Du , Yuhan Sui, Lina Chen

{"title":"Preparation, characterization and evaluation properties of a high-methoxyl pectin-iron (Ⅲ) from Actinidia arguta fruits","authors":"Tienan Wang , Xinning Du , Yuhan Sui, Lina Chen","doi":"10.1016/j.procbio.2025.06.013","DOIUrl":"10.1016/j.procbio.2025.06.013","url":null,"abstract":"<div><div>Iron deficiency anemia (IDA) was the most common nutritional disorder worldwide. As an effective and stable iron supplement, the physicochemical properties of iron complexes prepared using plant active ingredients have been extensively studied. In this study, a high methoxyl pectin AAP was extracted from the <em>Actinidia arguta</em> fruits. The synthesis process of AAP-iron (III) was optimized by single factor test and response surface methodology test. The physicochemical properties, structural characterization, and in vitro bioactivity were systematically investigated. Under the optimal synthesis conditions of pH 8.1, reaction time of 40 min, and temperature of 48 ℃, the iron content of AAP-iron (III) reached 26.62 ± 0.31 %. In addition, the -OH and -COOH groups in AAP coordinated with Fe<sup>3 +</sup> to form β-FeOOH groups. Compared with AAP, although the antioxidant capacity and α-glucosidase inhibitory activity of AAP-iron (III) were weakened, its α-amylase inhibitory activity was enhanced. In vitro digestion tests showed that AAP-iron (III) had a slow-release effect. Compared to FeSO<sub>4</sub>, AAP-iron (III) was more stable and not easily damaged by the simulated digestive solution. This study demonstrated that AAP-iron (III) was a promising new iron supplement.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 339-349"},"PeriodicalIF":3.7,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144481042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of exogenous combinations of fatty acids on the production of monensin by Streptomyces cinnamonensis 外源脂肪酸组合对肉桂链霉菌产莫能菌素的影响

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-23 DOI: 10.1016/j.procbio.2025.06.014

Shanfei Zhang , Zhenhua Wang , Dandan Tian , Yan Zhang , Chihe Sun , Qingming Hou , Jingle Liang , Qun Wu , Fubao Sun

{"title":"Effect of exogenous combinations of fatty acids on the production of monensin by Streptomyces cinnamonensis","authors":"Shanfei Zhang , Zhenhua Wang , Dandan Tian , Yan Zhang , Chihe Sun , Qingming Hou , Jingle Liang , Qun Wu , Fubao Sun","doi":"10.1016/j.procbio.2025.06.014","DOIUrl":"10.1016/j.procbio.2025.06.014","url":null,"abstract":"<div><div>Monensin is produced by the fermentation of <em>Streptomyces cinnamonensis</em>, which is in wide use in the agricultural and pharmaceutical industries. To improve the monensin yield, this study focused on evaluating the role of fatty acids in vegetable oil on the strain growth and secondary metabolism. Results showed that soybean oil was the most effective for monensin fermentation, followed by olive, peanut, camellia, corn, and linseed oils. Four fatty acids, oleic acid, linoleic acid, stearic acid, and palmitic acid, predominantly consisting of these vegetable oils, were observed to exert a positive effect on the monensin fermentation, thus resulting in the monensin titer increasing by 8-, 6-, 2-, and 3-fold, though far inferior to the soybean oil (10-fold). Optimization of fatty acid addition, comprising 1.38 % oleic, 2.78 % linoleic, 0.31 % stearic, and 0.50 % palmitic acid, elevated the monensin titer to 15.0 g/L. With a fed-batch fermentation in a 10-L tank, the fermentation reached 26.71 g/L of the monensin, above 10 % higher than that of the soybean oil. The suitable combination of fatty acids in vegetable oil was favorable for the microbial growth and monensin synthesis, which can shed light on the desirable application of different oil varieties in the industrial.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 350-358"},"PeriodicalIF":3.7,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144481040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polycaprolactone nanoparticles as a delivery vehicle for styrax liquidus: Therapeutic effects on ulcer and gastric cancer cells 聚己内酯纳米颗粒作为液体styrus的递送载体：对溃疡和胃癌细胞的治疗作用

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-23 DOI: 10.1016/j.procbio.2025.06.015

Eda Ülkeryıldız-Balçık , Hilal Sahin-Nadeem , Gulderen Cosgun , Dilara Konuk-Takma , Mahmut Yıldıztekin , Aleyna Gezen , Emine Yazici , Ece Oylumlu , Gamze Tanriover , Mehmet Torun

{"title":"Polycaprolactone nanoparticles as a delivery vehicle for styrax liquidus: Therapeutic effects on ulcer and gastric cancer cells","authors":"Eda Ülkeryıldız-Balçık , Hilal Sahin-Nadeem , Gulderen Cosgun , Dilara Konuk-Takma , Mahmut Yıldıztekin , Aleyna Gezen , Emine Yazici , Ece Oylumlu , Gamze Tanriover , Mehmet Torun","doi":"10.1016/j.procbio.2025.06.015","DOIUrl":"10.1016/j.procbio.2025.06.015","url":null,"abstract":"<div><div>Styrax liquidus (SL), a balsam from <em>Liquidambar orientalis</em> Mill., possesses high antioxidant, anti-inflammatory, anti-ulcer, and anti-cancer properties but is limited in food and medicine applications due to the immiscibility, high molecular size, and unable to pass the lipid membranes of the cells. Nano-delivery systems enhance bioavailability, stability, and controlled release of bioactive compounds. In this study, SL-loaded poly(ε-caprolactone) (PCL+SL) nanoparticles were manufactured by nanoprecipitation (NP) and flash nanoprecipitation (FNP) methods. Characterizations included size, PDI, zeta potential, morphology, encapsulation efficiency, and <em>in vitro</em> digestion release studies. SL concentration influenced nanoparticle properties, with FNP yielding efficient and size-controlled particles more readily. Spherical-smooth nanoparticles were produced within size ranges of 220–510 nm for NP and 171–383 nm for FNP, with narrow size distribution PDI (0.2 %). High encapsulation efficiencies and sustained gastric release (69–75 %) with low oral release (1.59–2.88 %) were observed. Optimal formulations showed a gastroprotective effect with high urease inhibition rates in range of 55.80 % - 54.69 % at 0.2 mg/mL concentration. An increased cytotoxicity effect of SL (IC<sub>50</sub> of 15.8 µg/mL, 81 % p < 0.0001) against AGS human gastric adenocarcinoma cells, followed by PCL+SL nanoparticles which have potential as “a delivery vehicle” in cancer treatment.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 42-55"},"PeriodicalIF":3.7,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144489595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anaerobic digestion of fats, oils, and greases (FOG) combined with carboxylic acids: Biogas kinetics, microbiome, and metabolic pathways 脂肪、油和油脂（FOG）与羧酸结合的厌氧消化：沼气动力学、微生物组和代谢途径

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-19 DOI: 10.1016/j.procbio.2025.06.011

Nandini Thakur , Ali S. Alkorbi , Mohammed Jalalah , Farid A. Harraz , Mostafa M. Koutb , El-Sayed Salama

{"title":"Anaerobic digestion of fats, oils, and greases (FOG) combined with carboxylic acids: Biogas kinetics, microbiome, and metabolic pathways","authors":"Nandini Thakur , Ali S. Alkorbi , Mohammed Jalalah , Farid A. Harraz , Mostafa M. Koutb , El-Sayed Salama","doi":"10.1016/j.procbio.2025.06.011","DOIUrl":"10.1016/j.procbio.2025.06.011","url":null,"abstract":"<div><div>The addition of carboxylic acids (C2 and C3) in anaerobic digestion (AD) can stimulate microbial growth and improve biogas production. Thus, C2 and C3 were added separately in two phases with an increasing fat, oil, and grease (FOG) concentration. The effects of C2 and C3 on biogas and volatile fatty acids (VFAs) production were studied. The microbial community shifts among bacteria and archaea were also examined, along with the metabolic pathways and functional enzymes. Biogas production during FOG digestion was increased by 1.69- and 1.47-fold upon the addition of C2 and C3, respectively, at the end of phase I. The accumulation of VFAs and long-chain fatty acids limited the biogas production in phase II. The <em>Clostridium</em> (28.8–48.3 %) and <em>Turicibacter</em> (20.1–8.7 %) were abundant in C2 and C3 amended FOG reactors. The addition of C3 also upsurged <em>Pseudigracilibacillus</em> (27.5 %) and <em>Thiopseudomonas</em> (9.08 %). <em>Methanosphaera</em> was the predominant archaea accounted for 79.1 % and 84.6 % in C2 and C3 amended reactors, respectively. The major predicted metabolic pathways were carbon metabolism (ko01200) and ABC transporters (ko02010). Glucosidase and coenzyme F<sub>420</sub> hydrogenase were the foremost bacterial and archaeal enzymes, respectively. Digestion of complex substrates (such as FOG) could be effectively enhanced by the addition of carboxylic acids.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 329-338"},"PeriodicalIF":3.7,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144481041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the interaction of preosteoblast cells with poly-L lysine surface-modified chitosan/hydroxyapatite scaffolds and their potential applications in bone tissue engineering 聚l赖氨酸表面修饰壳聚糖/羟基磷灰石支架与成骨前细胞的相互作用及其在骨组织工程中的潜在应用

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-19 DOI: 10.1016/j.procbio.2025.06.012

Tuğçe Kan Mutlu , Betül Mutlu Özçınar , İsmet Köksal , Rabia Çakır

{"title":"Investigating the interaction of preosteoblast cells with poly-L lysine surface-modified chitosan/hydroxyapatite scaffolds and their potential applications in bone tissue engineering","authors":"Tuğçe Kan Mutlu , Betül Mutlu Özçınar , İsmet Köksal , Rabia Çakır","doi":"10.1016/j.procbio.2025.06.012","DOIUrl":"10.1016/j.procbio.2025.06.012","url":null,"abstract":"<div><div>Bone is a multifunctional organ that undergoes constant structural and biological changes. In cases of damage due to trauma, cancer, infection or hormonal imbalances, medical intervention is required for bone regeneration. This study aims to develop a tissue scaffold that promotes bone tissue regeneration by enhancing cell adhesion, proliferation, and mineralization. For this purpose, tissue scaffolds with varying contents were produced using chitosan (Ch), hydroxyapatite (HA), and poly-L-lysine (PLL) as scaffold materials by freeze-drying method and characterized. In studies conducted to evaluate the biological activities of the scaffolds on MC3T3-E1 preosteoblast, the PLL-Ch/HA 1:2 scaffold exhibited approximately 20 % higher cell viability than the control on days 3 and 7 of extract analysis. In cells cultured on the scaffold, PLL-coated Ch/HA scaffolds showed a greater proliferative effect than uncoated Ch/HA scaffolds on day 7 of culture, resulting in a significant increase in cell viability. Furthermore, the observed increase in calcification and mineralization when cells were cultured on PLL-modified scaffolds could be attributed to PLL promoting cell adhesion and proliferation, resulting in increased calcium deposition. The surface modification of Ch/HA composite scaffolds with PLL has revealed optimal performance in bone tissue engineering due to their favorable performance.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"157 ","pages":"Pages 33-41"},"PeriodicalIF":3.7,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144489596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0