Process Biochemistry最新文献_第7页

SoluBat: A bidirectional mamba framework for high-throughput protein solubility prediction in bioprocess optimization SoluBat：用于生物工艺优化中高通量蛋白质溶解度预测的双向曼巴框架

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-02-01 Epub Date: 2025-12-11 DOI: 10.1016/j.procbio.2025.12.006

Linjie Wu , Chen Zhang , Zijian Hu , Xurong Wang , Hongrong Liu , Bin Zhu

{"title":"SoluBat: A bidirectional mamba framework for high-throughput protein solubility prediction in bioprocess optimization","authors":"Linjie Wu , Chen Zhang , Zijian Hu , Xurong Wang , Hongrong Liu , Bin Zhu","doi":"10.1016/j.procbio.2025.12.006","DOIUrl":"10.1016/j.procbio.2025.12.006","url":null,"abstract":"<div><div>Protein solubility prediction is a critical step in protein engineering and biomanufacturing, directly influencing wet-lab pre-screening costs and the efficiency of high-throughput R&D. To address the limitations of existing methods in computational efficiency and model interpretability, this study proposes SoluBat, a multimodal deep learning model based on a bidirectional Mamba architecture. SoluBat integrates protein sequence, evolutionary information (PSSM), and physicochemical properties, dynamically fusing these features via an efficient gating mechanism to achieve a balance between performance and energy consumption. Experimental results demonstrate that SoluBat achieves higher accuracy and Matthews correlation coefficient than mainstream deep learning and traditional methods on multiple public datasets, while markedly reducing computational resource consumption. In addition, the model is equipped with built-in per-residue attribution analysis, supporting rational protein design. SoluBat thus provides an efficient and interpretable computational solution for high-throughput protein solubility prediction and industrial applications.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"161 ","pages":"Pages 139-151"},"PeriodicalIF":4.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145786508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sustainable extraction of marine collagen and hydrolysates: Recent advances and challenges towards commercialization 海洋胶原蛋白和水解物的可持续提取：最近的进展和商业化的挑战

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-01-01 Epub Date: 2025-10-20 DOI: 10.1016/j.procbio.2025.10.005

Farhana Islam , Tanjina Tarannum , Mahdia Mahmud , Fahim Rahman , Nafisa Islam

{"title":"Sustainable extraction of marine collagen and hydrolysates: Recent advances and challenges towards commercialization","authors":"Farhana Islam , Tanjina Tarannum , Mahdia Mahmud , Fahim Rahman , Nafisa Islam","doi":"10.1016/j.procbio.2025.10.005","DOIUrl":"10.1016/j.procbio.2025.10.005","url":null,"abstract":"<div><div>Functional bioactive properties of collagen and its hydrolysates have boosted widespread applications of collagenous products extracted from natural sources. Growing interest in marine collagen has emerged due to its unique biochemical properties, compatibility with religious and cultural restrictions, and concerns over diseases linked to mammalian collagen. Furthermore, high availability of discarded marine by-products has facilitated various extraction techniques leading to conversion of low-cost by-products (e.g., fish skin, bones, scales, etc.) into high-value collagenous derivatives. Many innovative routes are being explored at different stages of extraction to enable sustainable production of bioactive collagens from marine sources, but most of these approaches remain at an early stage of development. This review summarizes conventional, enzymatic, physical-assisted, and green solvent-based methods for marine collagen and hydrolysate production, briefly discussing marine collagen market, challenges associated with commercializing marine collagen-based products, and highlighting opportunities to provide a clearer perspective. Enzyme-assisted methods, when coupled with physical- or solvent-assisted methods, balance yield, bioactivity, and sustainability, though large-scale validation is limited. Although this field has progressed significantly, widescale unified comparisons of bioactivity and yield are lacking. Future research should integrate techno-economic and life cycle analysis with pilot-scale studies, paving the way for industrial adoption of sustainable marine collagen.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"160 ","pages":"Pages 63-85"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145418736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of iron modified walnut shell biochar on the anaerobic digestion performance and microbial community structure of sludge 铁改性核桃壳生物炭对污泥厌氧消化性能及微生物群落结构的影响

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-01-01 Epub Date: 2025-11-09 DOI: 10.1016/j.procbio.2025.11.005

Ao Zheng , Juan Mei , Jingcheng Zheng , Quan Chen , Jiao Wu

{"title":"Effects of iron modified walnut shell biochar on the anaerobic digestion performance and microbial community structure of sludge","authors":"Ao Zheng , Juan Mei , Jingcheng Zheng , Quan Chen , Jiao Wu","doi":"10.1016/j.procbio.2025.11.005","DOIUrl":"10.1016/j.procbio.2025.11.005","url":null,"abstract":"<div><div>Composites exhibiting electron transport characteristics can enhance the methane production of anaerobic digestion (AD). In the present study, walnut shells were used to prepare biochar (BC group) and composite conductive material-magnetic biochar (MBC group), and the impact of these two types of biochar on sludge anaerobic digestion was investigated. The results showed that both materials promoted the degradation of organic matter, with cumulative methane production increasing by 32 % (MBC) and 21.5 % (BC) respectively compared to the control group. During the peak period of methane production, magnetic biochar was more efficient in promoting the generation and conversion of acetic acid, thereby increasing methane yield more efficiently. Magnetic biochar was proven to have higher electron transfer efficiency and enhanced electron transfer in the AD system. Microbial community analysis indicated that the addition of BC and MBC increased the relative abundance of electroactive bacteria like <em>f__Synergistaceae</em> and <em>f__Bacteroidetes_vadinHA17</em>, which were conducive to direct interspecies electron transfer (DIET). For all groups, the dominant archaea genera were <em>Methanosarcina</em> and <em>Methanosaeta</em>. Especially, MBC enriched the acetoclastic methanogens-<em>Methanosaeta</em>, which might be due to the loaded iron oxides facilitating the acetate conversion.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"160 ","pages":"Pages 244-254"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145526095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Silico-driven engineering of Halomonas elongata L-asparaginase: Towards enhanced proteolytic resistance in lymphoblastic leukemia 长盐单胞菌l -天冬酰胺酶的硅驱动工程：增强淋巴细胞白血病的蛋白水解抗性

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-01-01 Epub Date: 2025-10-29 DOI: 10.1016/j.procbio.2025.10.015

Maryam Samadaei Ghadikolaei , Sedigheh Asad , Vahideh Hassan-Zadeh

{"title":"In Silico-driven engineering of Halomonas elongata L-asparaginase: Towards enhanced proteolytic resistance in lymphoblastic leukemia","authors":"Maryam Samadaei Ghadikolaei , Sedigheh Asad , Vahideh Hassan-Zadeh","doi":"10.1016/j.procbio.2025.10.015","DOIUrl":"10.1016/j.procbio.2025.10.015","url":null,"abstract":"<div><div>The short half-life of L-asparaginase in leukemia patients due to serum proteases limits its therapeutic efficacy. This study aimed to enhance the proteolytic stability of <em>Halomonas elongata</em> L-asparaginase against trypsin. Using trRosetta, the enzyme’s 3D structure was modeled, and substrate-binding residues were identified via molecular docking. Candidate mutation sites were selected based on secondary structure, dimerization, trypsin cleavage motifs, and epitope mapping. A site-saturation mutagenesis library led to the Arg206Thr variant, which exhibited 1.7-fold higher specific activity (2400 U/mg)<strong>,</strong> ∼50 % longer serum half-life (3.47 h)<strong>,</strong> enhanced trypsin resistance, and anti-proliferative activity against K562 cells (IC50 = 1.45 U/mL)<strong>.</strong> In silico analyses confirmed improved structural stability and substrate binding. This work demonstrates that rational, structure-guided mutation can simultaneously improve enzyme stability and activity in a novel L-asparaginase, providing a promising candidate for therapeutic application in leukemia.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"160 ","pages":"Pages 206-217"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145526094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Start-up pathway of palladium-catalyzed methane-membrane biofilm reactor for Fe(II)EDTA-NO reduction 钯催化甲烷膜生物膜反应器还原Fe(II)EDTA-NO的启动途径

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-01-01 Epub Date: 2025-10-26 DOI: 10.1016/j.procbio.2025.10.007

Shuang Fu , Feiyue Qian , Junjie Zhao , Shuai Wang , Wang Zhao , Yixuan Yin , Jianfang Wang

{"title":"Start-up pathway of palladium-catalyzed methane-membrane biofilm reactor for Fe(II)EDTA-NO reduction","authors":"Shuang Fu , Feiyue Qian , Junjie Zhao , Shuai Wang , Wang Zhao , Yixuan Yin , Jianfang Wang","doi":"10.1016/j.procbio.2025.10.007","DOIUrl":"10.1016/j.procbio.2025.10.007","url":null,"abstract":"<div><div>The reaction rate and stability limit the application of the methane-based membrane biofilm reactor(CH<sub>4</sub>-MBfR) in the reduction of Fe(II)EDTA-NO. In this study, a palladium-catalyzed membrane was fabricated on a polypropylene (PP) membrane and applied to a CH<sub>4</sub>-MBfR. The effects of the Pd-CH<sub>4</sub>-MBfR on Fe(II)EDTA-NO reduction were evaluated by two start-up strategies: direct start-up by feeding only Fe(II)EDTA-NO, and enhanced start-up by sequentially feeding different concentrations of NaNO<sub>3</sub> and NaNO<sub>3</sub>+Fe(III)EDTA before introducing Fe(II)EDTA-NO. It was shown that the reduction of Fe(II)EDTA-NO by palladium-catalyzed membranes in the direct startup pathway was not significantly different from that of conventional PP membranes, with a nitrogen removal efficiency of 30 % in both cases, and no iron reduction was observed. In contrast, the nitrogen removal efficiency of Pd-CH<sub>4</sub>-MBfR in the enhanced start-up pathway was 60 %, and the iron reduction was about 10 %. The start-up methods had significant effects on the microbial community structure. The enhanced start-up method increased the relative abundance of methane-oxidizing denitrifying bacteria. The incorporation of metallic palladium facilitated the enrichment of <em>Pseudomonas</em>, <em>Methylomonas,</em> and <em>Methylomona</em>. The results support that the Pd-CH<sub>4</sub>-MBfR is a potentially sustainable and reliable catalytic platform for reducing Fe(II)EDTA-NO by an enhanced start-up pathway.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"160 ","pages":"Pages 218-228"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145526096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation of High Fischer ratio oligopeptides by the strategy of COF-TpPa-1 coated magnetic graphene oxide immobilized α-chymotrypsin and phenylalanine modified chitosan resin COF-TpPa-1包被磁性氧化石墨烯固定α-凝乳胰蛋白酶和苯丙氨酸修饰壳聚糖树脂制备高菲舍尔比寡肽

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-01-01 Epub Date: 2025-10-10 DOI: 10.1016/j.procbio.2025.10.002

Xueli Shi , Liting Shi , Yi Zhang , Yongxi He , Lingjiao Wang , Xuecheng Liu , Yonghong Zhou , Haoshuang Ju , Huihui Liu , Xiaolan Liu , Xiaojiao Sun

{"title":"Preparation of High Fischer ratio oligopeptides by the strategy of COF-TpPa-1 coated magnetic graphene oxide immobilized α-chymotrypsin and phenylalanine modified chitosan resin","authors":"Xueli Shi , Liting Shi , Yi Zhang , Yongxi He , Lingjiao Wang , Xuecheng Liu , Yonghong Zhou , Haoshuang Ju , Huihui Liu , Xiaolan Liu , Xiaojiao Sun","doi":"10.1016/j.procbio.2025.10.002","DOIUrl":"10.1016/j.procbio.2025.10.002","url":null,"abstract":"<div><div>High Fischer Ratio Oligopeptides (HFO) are bioactive compounds with health benefits and applications in nutrition and therapeutics. However, high cost in enzymatic hydrolysis and non-specific adsorption losses of branched chain amino acids (BCAAs) posed challenges to large-scale preparation of HFO. This study introduced an innovative approach involving immobilization of α-chymotrypsin on COF-coated magnetic graphene (MG@TpPa-1-α-CT) for the hydrolysis of whey protein, coupled with using phenylalanine-modified chitosan resin (PCCR) as specific adsorbent for aromatic amino acids (AAAs). MG@TpPa-1 demonstrated an impressive immobilized-capacity of 144 mg·g<sup>−1</sup> for α-CT, along with a Kₘ of 18.98 mg·mL<sup>−1</sup> and a Vₘₐₓ of 13.44 µmol·min<sup>−1</sup> and exhibited enhanced working stability. Leveraging n-π interactions, PCCR demonstrated exceptional adsorption capabilities of 30.2 mg·g<sup>−1</sup> and 8.2 mg·g<sup>−1</sup> for phenylalanine and leucine, and elevated the F-value of whey protein hydrolysate to 28.2. This study provided a novel strategy for HFO’s large-scale preparation.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"160 ","pages":"Pages 21-30"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145278027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Haematococcus pluvialis residue as a promising dye adsorbent: Enhanced by choline chloride:p-toluenesulfonic acid and cellulase treatment 雨红球菌渣作为一种有前途的染料吸附剂：经氯化胆碱、对甲苯磺酸和纤维素酶处理增强

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-01-01 Epub Date: 2025-10-28 DOI: 10.1016/j.procbio.2025.10.012

Yuya Liu , Fuzhen Liu , Zhan Wang, Huiling Huang, Jing Gao

{"title":"Haematococcus pluvialis residue as a promising dye adsorbent: Enhanced by choline chloride:p-toluenesulfonic acid and cellulase treatment","authors":"Yuya Liu , Fuzhen Liu , Zhan Wang, Huiling Huang, Jing Gao","doi":"10.1016/j.procbio.2025.10.012","DOIUrl":"10.1016/j.procbio.2025.10.012","url":null,"abstract":"<div><div>The cell wall of <em>Haematococcus pluvialis</em> (<em>H. pluvialis</em>) is rich in polysaccharides. After astaxanthin extraction, cellulose and hemicellulose remain abundantly present in the residue, which could form chemical interactions of hydroxyl (-OH), carbonyl (C<img>O) with dyes. In this study, <em>H. pluvialis</em> residue (HPR) was treated using acid deep eutectic solvent (DES) combined with cellulase at low temperature. The treatment parameters and adsorbent conditions were investigated to improve the removal rate of Congo red (CR) from water. The HPR-based biosorbent was characterized via SEM, FTIR, and elemental and BET analysis. After the treatment with choline chloride:p-toluenesulfonic acid (ChCl:pTSA) at 60 °C for 3 h and cellulase at 60 °C for 16 h, the specific surface area of the HPR was increased from 1.44 m<sup>2</sup>/g to 7.45 m<sup>2</sup>/g, while the pore volume increased from 0.0031 cm<sup>3</sup>/g to 0.0599 cm<sup>3</sup>/g, and the maximum removal rate of CR reached 95 % with a dosage of 0.67 g/L. The adsorption kinetic was better described by pseudo-second-order model. The Langmuir model provided the best fit and the maximum sorption capacity of 443.42 mg/g was recorded. The findings demonstrate that HPR treated with acid DES and cellulase can serve as an eco-friendly bioadsorbent for anionic dye removal.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"160 ","pages":"Pages 111-127"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145467334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced docosahexaenoic acid production of Schizochytrium sp. H016 utilizing rapeseed meal hydrolysate 利用菜籽粕水解物增强Schizochytrium sp. H016生产二十二碳六烯酸

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-01-01 Epub Date: 2025-10-30 DOI: 10.1016/j.procbio.2025.10.011

Yumei Feng , Yuanmin Zhu , Bohan Wang , Feifan Zhao , Yanxia Zhang , Xinqi Zhu , Huaixin Li , Longjiang Yu

{"title":"Enhanced docosahexaenoic acid production of Schizochytrium sp. H016 utilizing rapeseed meal hydrolysate","authors":"Yumei Feng , Yuanmin Zhu , Bohan Wang , Feifan Zhao , Yanxia Zhang , Xinqi Zhu , Huaixin Li , Longjiang Yu","doi":"10.1016/j.procbio.2025.10.011","DOIUrl":"10.1016/j.procbio.2025.10.011","url":null,"abstract":"<div><div>Rapeseed meal hydrolysate (RSMH), is a low-cost, nitrogen-rich substrate with high potential for microbial fermentation. This study investigated the feasibility of using RSMH as an alternative nitrogen source to enhance docosahexaenoic acid (DHA) production in <em>Schizochytrium</em> sp. H016. Nutritional analysis revealed that RSMH contains abundant amino acids and phenolics, which are favorable for microbial growth and stress tolerance. Fermentation results showed that replacing yeast extract with RSMH significantly improved biomass accumulation, lipid production, and DHA yield. At 120 h, the maximum biomass, lipid, and DHA yields reached 45.80 g/L, 18.62 g/L, and 7.30 g/L, respectively, corresponding to increases of 23.96 %, 97.66 %, and 94.15 % compared to the control. RT-qPCR analysis revealed that RSMH upregulated key genes involved in glycolysis, fatty acid biosynthesis, triacylglycerol assembly, and antioxidant defense, which together promoted carbon flux toward lipid synthesis and improved oxidative stress resistance. Confocal microscopy further confirmed the increased accumulation of lipid droplets in RSMH medium. Fed-batch fermentation in a 5-L bioreactor yielded DHA and lipids of 15.51 and 33.81 g/L, respectively, with a final biomass of 63.80 g/L. These findings demonstrate that RSMH effectively substitutes yeast extract and upgrades rapeseed meal into a feedstock for DHA production.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"160 ","pages":"Pages 102-110"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145418735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An innovative yeast-alginate-based system designed for the effective biosorption of cadmium, lead, and arsenate 一种创新的酵母-海藻酸盐为基础的系统设计的镉，铅和砷酸盐的有效生物吸附

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-01-01 Epub Date: 2025-11-10 DOI: 10.1016/j.procbio.2025.11.003

Carolina Moreno-Rivas , Jaqueline García-Hernández , Jose Andre-i Sarabia-Sainz , Alma Rosa Islas-Rubio , Luz Vázquez-Moreno , Tomás Jesús Madera-Santana , Patricia Quintana-Owen , Gabriela Ramos Clamont Montfort

{"title":"An innovative yeast-alginate-based system designed for the effective biosorption of cadmium, lead, and arsenate","authors":"Carolina Moreno-Rivas , Jaqueline García-Hernández , Jose Andre-i Sarabia-Sainz , Alma Rosa Islas-Rubio , Luz Vázquez-Moreno , Tomás Jesús Madera-Santana , Patricia Quintana-Owen , Gabriela Ramos Clamont Montfort","doi":"10.1016/j.procbio.2025.11.003","DOIUrl":"10.1016/j.procbio.2025.11.003","url":null,"abstract":"<div><div>This study developed two biosorption systems for removing heavy metals from water. System A utilized a yeast-alginate framework for cadmium (Cd²⁺) and lead (Pb²⁺), while System B employed an iron nanoparticle-functionalized yeast-alginate system for arsenate [As(V)]. Both systems were tested in a fixed-bed column. System A achieved removal efficiencies of 82.3 ± 1.5 % for cadmium and 91.7 ± 0.6 % for lead, whereas System B had a removal rate of 64.6 ± 1.5 % for arsenate. Two models were developed to assess simultaneous biosorption: Model 1 used tandem columns with separate systems, and Model 2 combined both systems in a single column. Model 1 outperformed Model 2, with biosorption capacities of 115.9 ± 0.9 µg/g for Cd²⁺, 195.1 ± 5.3 µg/g for Pb²⁺, and 71.0 ± 1.2 µg/g for [As(V)]. Hydrochloric acid (HCl) and acetic acid (CH₃COOH) were also investigated as potential desorption treatments, but yielded low results. The presence of common anions in water did not significantly affect biosorption. Overall, the tandem biosorption model seems to be a more practical, affordable, reusable, and eco-friendly option for effectively removing Cd²⁺, Pb²⁺, and [As(V)] from drinking water.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"160 ","pages":"Pages 229-243"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145526093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Self-assembling multi-enzyme complex for production of spermidine from putrescine 从腐胺中合成亚精胺的自组装多酶复合物

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-01-01 Epub Date: 2025-11-04 DOI: 10.1016/j.procbio.2025.11.002

Jiaxiang Yuan, Yi Liu, Xinfeng Hu, Jiao Feng, Kequan Chen, Xin Wang, Sheng Xu

{"title":"Self-assembling multi-enzyme complex for production of spermidine from putrescine","authors":"Jiaxiang Yuan, Yi Liu, Xinfeng Hu, Jiao Feng, Kequan Chen, Xin Wang, Sheng Xu","doi":"10.1016/j.procbio.2025.11.002","DOIUrl":"10.1016/j.procbio.2025.11.002","url":null,"abstract":"<div><div>Spermidine, a naturally occurring polyamine compound, has garnered considerable attention in biomedical research due to its lifespan-extending properties and potential applications in cancer therapeutics. Traditional biosynthetic methods, while environmentally friendly, suffer from prolonged reaction times. This study innovatively constructs a self-assembling multi-enzyme complex to enhance spermidine production from putrescine. In this study, a two-enzyme complex of spermidine synthase (SpeE) and S-adenosylmethionine decarboxylase (SpeD) was formed via the specific interaction between CohesinⅡ and DockerinⅡ to catalyze spermidine synthesis. The reaction time dropped sharply from 8 h in the free-enzyme system to just 3 h. Optimal spermidine-production conditions were determined by studying metal-ion additives, reaction temperature, and pH. Furthermore, a sophisticated multi-enzyme complex with cyanobacterial carboxysome protein (CcmK2) as a natural scaffold was developed. This CcmK2-SpeE-SpeD complex showed excellent stability and catalytic efficiency under harsh conditions, underscoring the protective role of protein scaffolds. Overall, this study offers an effective spermidine production strategy and highlights the potential of protein engineering and synthetic biology in enhancing biocatalytic processes for industrial uses.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"160 ","pages":"Pages 186-193"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145467333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0