Process Biochemistry最新文献_第8页

Enhanced glutathione production via precision cysteine feeding in whole-cell S. cerevisiae biocatalysis under oxidative stress 氧化应激下全细胞酿酒酵母生物催化中通过精确半胱氨酸喂养提高谷胱甘肽生产

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-01-01 Epub Date: 2025-10-28 DOI: 10.1016/j.procbio.2025.10.013

Juan S. Torres-Lucas , Nelson H. Caicedo-Ortega , Carlos Alvarez-Vasco

{"title":"Enhanced glutathione production via precision cysteine feeding in whole-cell S. cerevisiae biocatalysis under oxidative stress","authors":"Juan S. Torres-Lucas , Nelson H. Caicedo-Ortega , Carlos Alvarez-Vasco","doi":"10.1016/j.procbio.2025.10.013","DOIUrl":"10.1016/j.procbio.2025.10.013","url":null,"abstract":"<div><div>A whole-cell biocatalytic process was developed to transform surplus <em>Saccharomyces cerevisiae</em> biomass—such as brewers’ or distillers’ yeast—into a high-value, glutathione–rich ingredient. A central composite design (CCD) was used to maximize total glutathione accumulation, evaluating the effects of cysteine concentration and oxidative stress under two dosage strategies: single dosage and continuous feeding. Compared to baseline, resting (non-growing) yeast cells can achieve a four-fold increase in reduced and total glutathione. Cysteine uptake saturates at ∼11 mM, but a continuous feed of 13 mM cysteine with 1.2 mM hydrogen peroxide delivers the best performance for total and reduced glutathione (2.47 % and 1.59 %, respectively). Significant quadratic effects, particularly associated with cysteine and hydrogen peroxide concentrations, were observed, evidencing increased GSH accumulation at the central point of the design. The phenomenological analysis supported by multi-objective optimization demonstrated that it is not possible to simultaneously increase total and reduced glutathione. These findings validate using yeast as a whole-cell biocatalyst, under moderate oxidative conditions and controlled cysteine feeding, as a scalable strategy for producing GSH-enriched biomass. By repurposing waste yeast with minimal additional capital investment, breweries and wineries can unlock a new revenue stream and meet growing demand for natural antioxidant supplements.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"160 ","pages":"Pages 162-173"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145467376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating propionate production in Clostridium ljungdahlii inoculated bioelectrochemical system 永达梭菌接种生物电化学体系丙酸产量的评价

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-01-01 Epub Date: 2025-10-13 DOI: 10.1016/j.procbio.2025.10.003

Zhen Fang , Qichao Fan , Yu Huang , Beisong Xu , Zhi-Jie Yang , Yang Gu , Vinod Kumar , Yang-Chun Yong

{"title":"Evaluating propionate production in Clostridium ljungdahlii inoculated bioelectrochemical system","authors":"Zhen Fang , Qichao Fan , Yu Huang , Beisong Xu , Zhi-Jie Yang , Yang Gu , Vinod Kumar , Yang-Chun Yong","doi":"10.1016/j.procbio.2025.10.003","DOIUrl":"10.1016/j.procbio.2025.10.003","url":null,"abstract":"<div><div>Bioproduction of chemical building block such as propionic acid (propionate) is of great interest in current time as it is a sustainable alternative to petrochemical synthesis. <em>Clostridium ljungdahlii</em> as an electroactive homoacetogen is able to ferment sugars or utilize CO<sub>2</sub> to produce organic acids under bioelectrochemical system (BES). However, few reports evaluated the ability of bioelectrochemically assisted propionate production via <em>C. ljungdahlii</em>. In this study, BES equipped with three-dimensional nanostructure electrode and inoculated with <em>C. ljungdahlii</em> in cathode chamber was developed to evaluate propionate production. It found that applied negative potential on cathode was necessary to stimulate propionate production from fructose. Bioelectrochemical characterizations of bacteria attached electrode revealed that <em>C. ljungdahlii</em> was able to assimilate extracellular electron. Notably, optimization of negative potential on electrode remarkably increased NAD(P)H/NAD(P)<sup>+</sup> ratio, ATP amounts, and propionate production. Furthermore, BES optimization results showed that propionate achieved 16.29 ± 0.16 mM maximum titer (43 % ratio of total organic acids) and 0.2 g/g mass yield (0.24 mol/mol carbon atom yield) from fructose. A theoretical value (1.6 mmol) of fixed CO<sub>2</sub> was calculated from mass/electron balance analysis. This work not only evaluated the performance of bioelectrochemical technology but also provided a new choice for sustainable and efficient propionate production.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"160 ","pages":"Pages 11-20"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145278026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nano pearl powder/chitosan-hyaluronic acid scaffold promotes angiogenesis in vitro and in vivo 纳米珍珠粉/壳聚糖-透明质酸支架在体外和体内促进血管生成

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-01-01 Epub Date: 2025-10-30 DOI: 10.1016/j.procbio.2025.10.016

Yawen Wang , Rong She , Wenbai Zhang , Yanan Cheng , Yanlan Yang , Xinghuanyu Xu , Pu Xu

{"title":"Nano pearl powder/chitosan-hyaluronic acid scaffold promotes angiogenesis in vitro and in vivo","authors":"Yawen Wang , Rong She , Wenbai Zhang , Yanan Cheng , Yanlan Yang , Xinghuanyu Xu , Pu Xu","doi":"10.1016/j.procbio.2025.10.016","DOIUrl":"10.1016/j.procbio.2025.10.016","url":null,"abstract":"<div><div>The repair of bone defects represents a coordinated process entailing interactions, including such as early immune - inflammation regulation, angiogenesis, osteogenic differentiation, and bone formation, among angiogenesis is crucial. Consequently, augmenting the angiogenic capacity of engineering materials has emerged as a pivotal strategy for effective bone regeneration. In this study, a nano pearl powder (NPP)/chitosan-hyaluronic acid (C-HA) composite bioactive scaffold was cultured with human umbilical vein endothelial cells (HUVECs) to evaluate its angiogenic potential <em>in vitro</em>. The NPP/C-HA scaffolds promoted HUVEC proliferation, with a cell proliferation rate of 1.49 % ± 4.5 % and a cell count of 22 ± 1.73 per field of view. Tubule formation was observed, with 101 ± 33 junction points and 78 ± 14 branch points. The relative protein expression levels of vascular endothelial growth factor (VEGF), vascular endothelial growth factor receptor 2 (VEGFR2), and basic fibroblast growth factor (bFGF) were increased. <em>In vivo</em>, the addition of NPP to the scaffolds enhanced the expression of vascular-related proteins, including platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) and VEGF. Simultaneously, microvessel density (MVD) increased to 19.51 ± 4.60. These findings suggest that NPP/C-HA may be a promising biomaterial for bone repair and regeneration.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"160 ","pages":"Pages 150-161"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145467324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sustainable eco-friendly alginate-residue composite biosorbents from marine waste Sargassum siliquosum for high efficiency Cr(VI) removal 可持续的生态友好型海藻酸渣复合生物吸附剂从海洋废物马尾藻硅藻土高效去除Cr（VI）

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-01-01 Epub Date: 2025-10-30 DOI: 10.1016/j.procbio.2025.10.014

Guruvignesh Senthilkumar , Dillirani Nagarajan , Thanh-Binh Nguyen , Balamurugan Rathinam , Chiu-Wen Chen , Jo-Shu Chang , Cheng-Di Dong

{"title":"Sustainable eco-friendly alginate-residue composite biosorbents from marine waste Sargassum siliquosum for high efficiency Cr(VI) removal","authors":"Guruvignesh Senthilkumar , Dillirani Nagarajan , Thanh-Binh Nguyen , Balamurugan Rathinam , Chiu-Wen Chen , Jo-Shu Chang , Cheng-Di Dong","doi":"10.1016/j.procbio.2025.10.014","DOIUrl":"10.1016/j.procbio.2025.10.014","url":null,"abstract":"<div><div>This study investigated the application of low-cost marine-derived biosorbents to effectively remove higher concentrations (up to 200 mgL<sup>−1</sup>) of Cr(VI) in aqueous solutions. Brown seaweed <em>Sargassum siliquosum</em> was collected from the Southern Taiwan coastal areas. Extracted alginate and the extractive residue (post-alginate extraction) were used to prepare the alginate (EA) - alginate extraction residue (AR) composite beads using calcium chloride as a crosslinker. Maximum Cr (VI) removal of > 90 % was obtained at pH 2 with up to 200 mgL<sup>−1</sup> Cr(VI), indicating the high efficiency and tolerance of the beads. The optimum conditions for maximum Cr(VI) adsorption were pH 2, 100 mgL<sup>−1</sup> Cr(VI), and 1:10 biosorbent dosage, attaining a removal efficiency of 99.31 ± 0.71 %. The biosorbents can be reused for up to five cycles without a considerable loss of removal efficiency. Elemental mapping revealed the biosorption of Cr onto the bio-composite beads, with some changes in ionic composition, suggesting ion exchange. XPS analysis demonstrated that the absorbed Cr(VI) was reduced to non-toxic Cr(III) to some extent. This study presents an efficient method for upcycling marine waste and identifying prospective applications in environmental remediation, with the potential to treat real-world wastewaters.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"160 ","pages":"Pages 136-149"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145467330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of type of organic solid waste and process temperature on lactate-driven hydrogen production and microbial community by self-fermentation 有机固体废弃物类型和工艺温度对乳酸产氢及微生物群落的影响

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-01-01 Epub Date: 2025-10-10 DOI: 10.1016/j.procbio.2025.10.004

Marisol Pérez-Rangel, Edith Villanueva-Galindo, Idania Valdez-Vazquez, Iván Moreno-Andrade

{"title":"Influence of type of organic solid waste and process temperature on lactate-driven hydrogen production and microbial community by self-fermentation","authors":"Marisol Pérez-Rangel, Edith Villanueva-Galindo, Idania Valdez-Vazquez, Iván Moreno-Andrade","doi":"10.1016/j.procbio.2025.10.004","DOIUrl":"10.1016/j.procbio.2025.10.004","url":null,"abstract":"<div><div>Organic solid waste (OSW), depending on its origin, shows differences in the content of carbohydrates, lipids, and proteins, influencing its potential for valorization. This study aimed to evaluate the influence of three types of OSW (restaurant food waste (RFW), market waste (MW), and the organic fraction of municipal solid waste (OFMSW)) and temperature (37 and 55°C) on hydrogen production, metabolites production, and microbial community composition. The native microbiota of each OSW served as inoculum. Results confirmed that the type of feedstock significantly influenced lactate-based hydrogen production, which was temperature-dependent. Mesophilic self-fermentation of OFMSW yielded the highest hydrogen production, reaching 257 mL H<sub>2</sub>/L, 23 % higher than the obtained at thermophilic conditions and 71 % higher than that obtained from MW at mesophilic conditions. Hydrogen production of OFMSW at both 37°C and 55°C and MW at 37°C was correlated with the butyric acid production and the presence of the <em>Clostridium</em> genus. In contrast, RFW at 37 and 55 °C was associated with lactic acid production and a higher abundance of <em>Lactobacillus</em> and <em>Bacillus</em>, respectively. Results demonstrated that OFMSW can be directly converted to hydrogen, while RFW produces lactic acid, which could serve as a precursor for lactate-based hydrogen production in a two-stage process.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"160 ","pages":"Pages 1-10"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145278028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ARTP mutagenesis-driven γ-decalactone overproduction in Clavispora lusitaniae: Mechanistic insights from genomic and transcriptomic analyses ARTP诱变驱动的γ-内酯过量产生：来自基因组和转录组学分析的机制见解

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-01-01 Epub Date: 2025-11-04 DOI: 10.1016/j.procbio.2025.10.017

Wenbin Ren , Jun Wang , Xuan Xin , Wei Wang , Weidong Bai , Mengmeng Zhang , Wenhong Zhao

{"title":"ARTP mutagenesis-driven γ-decalactone overproduction in Clavispora lusitaniae: Mechanistic insights from genomic and transcriptomic analyses","authors":"Wenbin Ren , Jun Wang , Xuan Xin , Wei Wang , Weidong Bai , Mengmeng Zhang , Wenhong Zhao","doi":"10.1016/j.procbio.2025.10.017","DOIUrl":"10.1016/j.procbio.2025.10.017","url":null,"abstract":"<div><div>γ-Decalactone (GDL), a natural fragrance with applications in food, cosmetics, and therapeutics, faces production challenges via chemical synthesis due to racemization and environmental concerns. Microbial fermentation offers a sustainable alternative, yet requires high-yielding strains. This study employed atmospheric and room temperature plasma (ARTP) mutagenesis to enhance GDL production in <em>Clavispora lusitaniae</em>. Among the numerous mutants, A69 exhibited a 36 % increase in GDL titer (1.57 g/L) after 48 h of fermentation compared to the parental strain (1.15 g/L) and maintained genetic stability across ten generations. Following fermentation condition optimization, mutant A69 reached a maximum GDL production of 1.99 g/L at 48 h, and 3.78 g/L at 8th day. Genomic and transcriptomic analyses revealed that the enhanced GDL production in A69 may be associated with mutations in β-oxidation-related genes (<em>POX1</em>) and upregulation of fatty acid degradation-related genes (<em>ALD4</em>, <em>FAA2</em>). This study proposes novel strategies to enhance microbial GDL production.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"160 ","pages":"Pages 174-185"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145467332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing Wolffia globosa cultivation: Feasibility of aerated suspended-growth systems as alternative cultivating option and the influence of abiotic factors on biomass production, CO₂ capture, and biochemical composition 加强全球沃尔夫的培养：曝气悬浮生长系统作为替代培养方案的可行性以及非生物因素对生物量生产、CO 2捕获和生化组成的影响

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-01-01 Epub Date: 2025-10-15 DOI: 10.1016/j.procbio.2025.10.006

Kittikoon Sucunthowong , Shiva Rezaei Motlagh , Jareeluk Plengsakul , Sorawit Powtongsook , Kasidit Nootong

{"title":"Enhancing Wolffia globosa cultivation: Feasibility of aerated suspended-growth systems as alternative cultivating option and the influence of abiotic factors on biomass production, CO₂ capture, and biochemical composition","authors":"Kittikoon Sucunthowong , Shiva Rezaei Motlagh , Jareeluk Plengsakul , Sorawit Powtongsook , Kasidit Nootong","doi":"10.1016/j.procbio.2025.10.006","DOIUrl":"10.1016/j.procbio.2025.10.006","url":null,"abstract":"<div><div>This study evaluated the feasibility of well-mixed suspended-growth systems for cultivating <em>Wolffia globosa</em> and investigated the effects of abiotic factors on growth, CO<sub>2</sub> capture, and biochemical composition of <em>Wolffia</em>. Continuous aeration at 0.4 and 0.8 vvm significantly improved biomass productivity compared to static water cultivation systems, while continuous stirring at 600 rpm caused extensive frond damage and lower biomass concentrations. Under optimized aerated conditions (500 mg/L initial biomass, 80 mg N/L initial nitrate, and 402 μmol photon/m<sup>2</sup>·s light intensity), the maximum biomass productivity reached 199.94 ± 25.88 mg/L·d, representing 83–277 % increase over static water cultivation. The corresponding CO<sub>2</sub> fixation rate (283.71 ± 36.73 mg CO<sub>2</sub>/L·d) was comparable to those of <em>Chlorella</em>, highlighting the potential of <em>W. globosa</em> for biological CO<sub>2</sub> mitigation. Protein contents under optimized condition was 21.5 % of dry weight, with nitrate availability positively influencing protein accumulation. Key carotenoids, including lutein, β-carotene, neoxanthin, and violaxanthin, were detected, with no significant difference (p > 0.05) between suspended growth and static water systems. Furthermore, due to large frond size, biomass harvesting can be effectively conducted using filtration with low-cost, locally available materials.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"160 ","pages":"Pages 31-40"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145326751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New biosensor method as a first step in assessing microbial metabolism: Case study of Rhodococcus methylotrophy 新的生物传感器方法作为评估微生物代谢的第一步：甲基化红球菌的案例研究

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-01-01 Epub Date: 2025-11-02 DOI: 10.1016/j.procbio.2025.11.001

Elena Emelyanova

{"title":"New biosensor method as a first step in assessing microbial metabolism: Case study of Rhodococcus methylotrophy","authors":"Elena Emelyanova","doi":"10.1016/j.procbio.2025.11.001","DOIUrl":"10.1016/j.procbio.2025.11.001","url":null,"abstract":"<div><div>The new simple and easy-to-use electrochemical biosensor method has not been previously used to assess the growth substrate for <em>Rhodococcus</em> methylotrophs. This method implies the combined use of laboratory models of microbial sensor devices to evaluate the constitutiveness and inducibility of enzymes of the initial metabolism of substrate. Earlier, two facultative methylotrophs were compared using traditional methods: <em>Rhodococc</em>us <em>wratislaviensis</em> VKM Ac-2782 was able to grow in the methanol-containing medium, no growth of <em>Rhodococcus koreensis</em> DNP505<sup>T</sup> was observed. In this study, using the biosensor approach, it was shown that differences in growth of cultures were due to the presence/absence of the enzyme of the initial metabolism of methanol. The existence of the constitutive enzyme of the initial metabolism of methanol predetermined the growth of <em>R. wratislaviensis</em> in the presence of methanol. Methanol penetrated into <em>R. koreensis</em> cells; but the culture did not contain the enzyme for the first stage of methanol metabolism. As a result, <em>R. koreensis</em> did not grow in the methanol-containing medium. The obtained results confirm that the new biosensor method is an indispensable tool for assessing the availability of substrate for microbial cultures, significantly facilitating research in the field of taxonomy, microbial bioremediation and fermentation of microorganisms.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"160 ","pages":"Pages 128-135"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145467331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whole-cell biotechnological applications with thermoacidophilic Crenarchaeota: Opportunities and challenges 嗜热酸性绿藻的全细胞生物技术应用：机遇与挑战

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-01-01 Epub Date: 2025-10-19 DOI: 10.1016/j.procbio.2025.10.008

Longinus Ifeanyi Igbojionu , Marta Maso Martinez , Alan D. Goddard , Alfred Fernandez-Castane

{"title":"Whole-cell biotechnological applications with thermoacidophilic Crenarchaeota: Opportunities and challenges","authors":"Longinus Ifeanyi Igbojionu , Marta Maso Martinez , Alan D. Goddard , Alfred Fernandez-Castane","doi":"10.1016/j.procbio.2025.10.008","DOIUrl":"10.1016/j.procbio.2025.10.008","url":null,"abstract":"<div><div>Extremophiles are microorganisms that thrive in harsh environmental conditions where no life exists. These environmental conditions can involve temperature, acidity, salinity, pressure, or radiation that are typically uninhabitable to most microorganisms. The thermoacidophilic Archaea (e.g., <em>Acidianus</em>, <em>Metallosphaera</em> and <em>Sulfolobus)</em> can thrive at high temperatures and low pHs. These organisms are capable of autotrophic, lithotrophic, heterotrophic, chemoheterotrophic, and chemolithoautotrophic lifestyles, and thus can easily be cultivated on many different substrates. Additionally, their innate capacities to oxidise ferrous iron and/or reduced inorganic sulfur compounds have gained recognition for their utility in biomining operations. Members of <em>Acidianus</em> and <em>Metallosphaera</em> have been applied in bioleaching operations to extract valuable metals from low-grade ores and mineral concentrates. The advances in <em>Sulfolobus</em> genetics have presented opportunities for their application as platform organisms in biotechnological and biorefinery processes due to the ability to cultivate these extremophilic organisms under non-sterile conditions. Furthermore, utilising inexpensive and sustainable feedstock such as lignocellulosic biomass is one key advantage of lowering cultivation costs. This review presents current research developments on thermoacidophilic Sulfolobales members, emphasising their whole-cell applications for biomining operations, biotechnology and biorefinery. Several laboratory-scale studies found these organisms promising for large-scale deployment based on their unique characteristics.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"160 ","pages":"Pages 86-101"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145418734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing microbial induced calcium carbonate precipitation (MICCP) in self-healing concrete by addressing cell encapsulation: Investigating the impact of exogenous carbonate ions 通过处理细胞封装来增强自愈混凝土中微生物诱导的碳酸钙沉淀（MICCP）：研究外源碳酸盐离子的影响

IF 4 3区生物学

Process Biochemistry Pub Date : 2026-01-01 Epub Date: 2025-10-30 DOI: 10.1016/j.procbio.2025.10.010

Seyed Ali Rahmaninezhad , Mohammad Houshmand , Parsa Namakiaraghi , Amirreza Sadighi , Kiana Ahmari , Divya Kamireddi , Yaghoob (Amir) Farnam , Caroline L. Schauer , Ahmad R Najafi , Christopher M. Sales

{"title":"Enhancing microbial induced calcium carbonate precipitation (MICCP) in self-healing concrete by addressing cell encapsulation: Investigating the impact of exogenous carbonate ions","authors":"Seyed Ali Rahmaninezhad , Mohammad Houshmand , Parsa Namakiaraghi , Amirreza Sadighi , Kiana Ahmari , Divya Kamireddi , Yaghoob (Amir) Farnam , Caroline L. Schauer , Ahmad R Najafi , Christopher M. Sales","doi":"10.1016/j.procbio.2025.10.010","DOIUrl":"10.1016/j.procbio.2025.10.010","url":null,"abstract":"<div><div>This study investigates the impact of calcium ion-rich media on biomineralization yield during microbially-induced calcium carbonate precipitation (MICCP) reactions, presenting a novel approach to enhance crack-filling kinetics crucial for concrete self-healing. Our findings reveal that bacterial cells, nucleation sites for calcium carbonate precipitation dominating their surfaces, become encapsulated by calcium carbonate crystals, particularly in calcium-rich environments. This encapsulation was hypothesized to impede cell access to nutrients and urea, resulting in significant reductions in cell growth (98.4 %), urea hydrolysis (84.6 %), and calcium carbonate production (81.2 %), delaying MICCP initiation by up to 10 days. Moreover, the consumption of dissolved carbonate ions due to calcium carbonate precipitation diminishes media buffering capacity, further retarding bacterial growth. Conversely, adding exogenous sodium carbonate accelerated bacterial growth (up to 122-fold), urea hydrolysis, and biomineralization (up to 10-fold), expediting crack-filling in cement mortar specimens. We also found that higher initial cell concentrations (OD600 of 1) provided more nucleation sites, reducing cell encapsulation and synergistically enhancing MICCP kinetics while maintaining media buffering capacity. These findings offer mechanistic insight into the importance of mitigating cell encapsulation during MICCP to enhance concrete crack-filling.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"160 ","pages":"Pages 194-205"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145467323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0