Process Biochemistry最新文献_第8页

Combinatorial engineering of microbial cell factory for 2′-fucosyllactose biosynthesis 2′-焦酰基乳糖生物合成微生物细胞工厂的组合工程

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-04 DOI: 10.1016/j.procbio.2025.05.024

Jingyu Zhou , Xinyue Su , Yongzhong Wang , Qiang Ding

{"title":"Combinatorial engineering of microbial cell factory for 2′-fucosyllactose biosynthesis","authors":"Jingyu Zhou , Xinyue Su , Yongzhong Wang , Qiang Ding","doi":"10.1016/j.procbio.2025.05.024","DOIUrl":"10.1016/j.procbio.2025.05.024","url":null,"abstract":"<div><div>2’-fucosyllactose (2’-FL), a predominant human milk oligosaccharide (HMOs), has showed significant potential in infant health applications including intestinal protection, cognitive development, and immune system modulation. Therefore, to enhance microbial biosynthesis of 2’-FL, we engineered a de novo synthesis pathway through deleting key enzymes gene: <em>wecB</em> (encoding UDP-N-acetyl glucosamine-2-epimerase), <em>lacZ</em> (encoding β-galactosidase), <em>wcaJ</em> (encoding UDP-glucose lipid carrier transferase), which could increase the 2’-FL titer and productivity to 2.25 g/L and 0.03 g/L/h, respectively. Furthermore, optimization of induction time further improved production performance to 4.06 g/L and 0.06 g/L/h. Through systematic gene deletion analysis, we identified <em>lacA</em> and <em>iclR</em> as critical metabolic nodes. Their knockout negatively impacted 2’-FL biosynthesis by reducing lactose availability and disturbing metabolic flux. Finally, the 2’-FL was furthermore improved to 4.75 g/L through expressing <em>gsk</em> and <em>zwf</em> genes for NADPH and GTP regeneration. This study can effectively increase the production of 2’-fucosyllactose, which can provide a research basis for the large-scale bioproduction of 2’-fucosyllactose in the industrial factory.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 202-208"},"PeriodicalIF":3.7,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144230402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tannic acid degradation potential and biochemical characterization of Paenibacillus lautus BCA501 isolated from the gut of Silver therapon (Leiopotherapon plumbeus) 银鲢肠道中劳氏芽孢杆菌BCA501单宁酸降解潜力及生化特性研究

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-04 DOI: 10.1016/j.procbio.2025.05.022

Nico G. Dumandan , Christian James B. Raiz , Annie Cita T. Kagaoan , Leo Joseph F. Labitag , Jay Ronel V. Conejos , Francisco B. Elegado , Amelia B. Hizon-Fradejas , Annabelle T. Abrera , Sheryl Lozel B. Arreola

{"title":"Tannic acid degradation potential and biochemical characterization of Paenibacillus lautus BCA501 isolated from the gut of Silver therapon (Leiopotherapon plumbeus)","authors":"Nico G. Dumandan , Christian James B. Raiz , Annie Cita T. Kagaoan , Leo Joseph F. Labitag , Jay Ronel V. Conejos , Francisco B. Elegado , Amelia B. Hizon-Fradejas , Annabelle T. Abrera , Sheryl Lozel B. Arreola","doi":"10.1016/j.procbio.2025.05.022","DOIUrl":"10.1016/j.procbio.2025.05.022","url":null,"abstract":"<div><div>Microbial degradation of tannic acid offers significant potential for mitigating the negative effects of tannins while enabling various biotechnological applications. In this study, <em>Paenibacillus lautus</em> BCA501, isolated from the gut of the Silver therapon (<em>Leiopotherapon plumbeus</em>), is reported for the first time as a tannic acid-degrading bacterium. The strain effectively utilized tannic acid for growth, achieving a biomass yield coefficient (Y<sub>X/S</sub>) of 1.30 g<sub>biomass</sub> per g<sub>substrate</sub> at 2 g/L tannic acid, which decreased to 0.594 g<sub>biomass</sub> per g<sub>substrate</sub> at 10 g/L. Kinetic analysis revealed a maximum growth rate of 0.562h<sup>-1</sup>, with substrate inhibition at 11.26 g/L based on the Monod model. Tannase activity, though growth-associated, declined from 0.300 to 0.046 U/mL with increasing substrate concentration. Despite this reduction, gallic acid release increased significantly, with its growth-associated production coefficient peaking at 2.22 g/L at 10 g/L tannic acid, demonstrating efficient hydrolysis into phenolic compounds. This study provides the first evidence of tannic acid degradation by <em>Paenibacillus lautus</em> underscoring its potential for microbial tannase production and phenolic biotransformation.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 236-243"},"PeriodicalIF":3.7,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144241089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Progress in chitosan-based materials: Enhancing edible coatings and films through modifications and functionalization for food preservation 壳聚糖基材料的进展：通过改性和功能化来增强可食用涂层和薄膜用于食品保鲜

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-03 DOI: 10.1016/j.procbio.2025.06.001

Maricarmen Iñiguez-Moreno , Josué David Hernández-Varela , Manuel Burelo , Joel H. Elizondo-Luevano , Rafael G. Araújo , Cecilia D. Treviño-Quintanilla , Dora I. Medina

{"title":"Progress in chitosan-based materials: Enhancing edible coatings and films through modifications and functionalization for food preservation","authors":"Maricarmen Iñiguez-Moreno , Josué David Hernández-Varela , Manuel Burelo , Joel H. Elizondo-Luevano , Rafael G. Araújo , Cecilia D. Treviño-Quintanilla , Dora I. Medina","doi":"10.1016/j.procbio.2025.06.001","DOIUrl":"10.1016/j.procbio.2025.06.001","url":null,"abstract":"<div><div>Chitosan has emerged as a versatile biopolymer with significant potential in food technology, particularly for developing edible coatings aimed at preserving perishable foods. This review highlights recent advancements in chitosan extraction, modification, and functionalization, emphasizing its applications in extending the shelf life of fruits, vegetables, meat, seafood, and dairy products. Chitosan-based coatings effectively reduce moisture, oxygen, and carbon dioxide permeability, thereby delaying oxidation and respiration. Their functionality can be further enhanced by incorporating nanomaterials and bioactive compounds, which improve mechanical strength, antimicrobial efficacy, and antioxidant activity while preserving the physicochemical and sensory quality of food. The development of smart chitosan coatings, responsive to changes in pH, temperature, and humidity, offers innovative possibilities for real-time food quality monitoring. Although chitosan is generally recognized as safe (GRAS), long-term toxicity studies and regulatory harmonization are necessary to ensure consumer safety. Moreover, alternative sources such as fungal-derived chitosan present sustainable, hypoallergenic options that help address the limitations of traditional crustacean-based chitosan. Overall, the use of chitosan in food preservation not only supports improved product quality and safety but also contributes to environmentally responsible and health-conscious packaging approaches, aligning with current sustainability goals in the food industry.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 175-190"},"PeriodicalIF":3.7,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144205772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of high hydrostatic pressure on Alcalase-assisted hydrolysis of soy protein isolate and the anti-aging activity of the hydrolysates 高静水压力对alcalase辅助水解大豆分离蛋白及其抗衰老活性的影响

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-06-01 DOI: 10.1016/j.procbio.2025.05.020

Xiaorui Liu , Lidan Pan , Yaxin Cheng , Die Dong , Haibo Zhao , Xiuzhen Ding , Chao Yuan , Bo Cui

{"title":"Effect of high hydrostatic pressure on Alcalase-assisted hydrolysis of soy protein isolate and the anti-aging activity of the hydrolysates","authors":"Xiaorui Liu , Lidan Pan , Yaxin Cheng , Die Dong , Haibo Zhao , Xiuzhen Ding , Chao Yuan , Bo Cui","doi":"10.1016/j.procbio.2025.05.020","DOIUrl":"10.1016/j.procbio.2025.05.020","url":null,"abstract":"<div><div>In this study, soy protein isolate hydrolysates (SPIH) with enhanced antiaging activity were produced. SPIH were generated by Alcalase hydrolysis of soy protein isolate (SPI) pretreated under high hydrostatic pressure (HHP: 200–500 MPa, 5–15 min). Results showed HHP treatment led to the transition of secondary structures of SPI, withα-helix and β-sheet transforming into random coil and β-turn. The dense network structure of SPI was disrupted, increasing the hydrolysis degree by 19.2 % (300 MPa, 5 min). Attributed to the radical scavenging effect by the surface exposure of hydrophobic residues, the antioxidant activity of HHP-treated SPIH were increased and reached a peak under the condition of 300 MPa for 5 min. In yeast cell anti-aging assays, HHP-treated SPIH decreased the accumulation of reactive oxygen species and malondialdehyde content, thereby delaying cellular aging. These findings demonstrated that HHP-assisted enzymatic hydrolysis enhanced the yield and bioactivity of anti-aging ingredients derived from SPI, providing a novel strategy for developing functional food.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 191-201"},"PeriodicalIF":3.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144203957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

On digital twins in bioprocessing: Opportunities and limitations 生物加工中的数字孪生：机遇与局限

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-05-31 DOI: 10.1016/j.procbio.2025.05.023

Mehrdad Shariatifar , Mohammadsadegh Salimian Rizi , Rahmat Sotudeh-Gharebagh , Reza Zarghami , Navid Mostoufi

{"title":"On digital twins in bioprocessing: Opportunities and limitations","authors":"Mehrdad Shariatifar , Mohammadsadegh Salimian Rizi , Rahmat Sotudeh-Gharebagh , Reza Zarghami , Navid Mostoufi","doi":"10.1016/j.procbio.2025.05.023","DOIUrl":"10.1016/j.procbio.2025.05.023","url":null,"abstract":"<div><div>Integrating Digital Twins (DTs) in bioprocessing has become a prominent focus within the industry. Despite the challenges associated with implementing this technology in the field, the bioprocessing sector is interested in utilizing it. This is due to its potential to enhance process efficiency and overall profitability. The adoption of DTs is driven by the prospect of online monitoring, control, and optimization, enabling the products with precise and desired characteristics. To realize this objective, researchers propose a novel strategy for implementing DTs in bioprocessing. This involves the development of a hybrid model that combines first principal models and Machine Learning (ML) algorithms. This approach effectively addresses the limitations of previous methods and establishes a closed control loop system, continuously monitoring the system and adjusting input variables to achieve optimal outcomes. This study comprehensively explores various aspects of DTs. Firstly, it discusses the concept and characteristics of DTs, along with an examination of the advantages and challenges associated with their implementation. Secondly, it comprehensively analyzes key factors that directly influence DT implementation, including sensors, data collection, and models. Thirdly, it reviews the implications of Digital Solutions (DS) and DT in downstream and upstream bioprocessing. By providing theories, case studies, and practical frameworks, this work seeks to motivate both researchers and industry practitioners to adopt DT methodologies, thereby facilitating the emergence of enhanced precision, operational efficiency, and economic viability within biomanufacturing.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 274-299"},"PeriodicalIF":3.7,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144270006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photocatalytic dye detoxification by cost-effective SnO2: Ce thin films and verification of toxicity by histology using Labeo rohita fish 采用高性价比的SnO2: Ce薄膜对光催化染料进行脱毒，并通过组织学方法验证Labeo rohita鱼的毒性

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-05-28 DOI: 10.1016/j.procbio.2025.05.014

K. Ravichandran , K. Gayathri , M. Baneto , B. Kaleeswaran , P.K. Praseetha , B. Deepa , N. Anuradha

{"title":"Photocatalytic dye detoxification by cost-effective SnO2: Ce thin films and verification of toxicity by histology using Labeo rohita fish","authors":"K. Ravichandran , K. Gayathri , M. Baneto , B. Kaleeswaran , P.K. Praseetha , B. Deepa , N. Anuradha","doi":"10.1016/j.procbio.2025.05.014","DOIUrl":"10.1016/j.procbio.2025.05.014","url":null,"abstract":"<div><div>This study is aimed to provide detailed insights into the photocatalytic degradation ability of cerium-doped tin oxide (SnO<sub>2</sub>: Ce) thin films fabricated using a cost-effective nebulizer spray pyrolysis technique. Characterization of the deposited samples was performed using XRD, SEM, EDS, E-mapping, XPS, PL and UV and the obtained results were correlated with the photocatalytic dye detoxification results. The effect of cerium doping at different concentrations (2.5, 5.0 and 7.5 wt%) on the ability of the films to break down methylene blue (MB), a toxic dye commonly found in industrial effluents was studied. The toxicity of the water treated with the doped films was evaluated by exposing freshwater fish <em>Labeo rohita</em> to the treated water and performing the histology study of different organs of the test fish using the paraffin wax method. The results were compared with the toxicity observed in the fish exposed to untreated methylene blue dye solution. The photocatalytic studies revealed a significant enhancement in the photocatalytic dye degrading efficiency of SnO<sub>2</sub> with the addition of Ce. The SnO<sub>2</sub>: Ce (5 %) film exhibited 96.44 % decomposition of Methylene Blue (MB) dye molecules.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 118-135"},"PeriodicalIF":3.7,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144178795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fiber composite hydrogels: Advances in food preservation and biomedical applications 纤维复合水凝胶：食品保鲜和生物医学应用的进展

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-05-28 DOI: 10.1016/j.procbio.2025.05.019

Xiaowei Xu , Qian Ning , Hanbin Chen , Jie Pang

引用次数: 0

SpyTag/SpyCatcher-mediated SpyRing cyclization improves the operational stability and renaturation efficiency of immobilized enzymes after catalytic inactivation SpyTag/ spycatcher介导的SpyRing环化提高了固定化酶在催化失活后的操作稳定性和还原效率

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-05-27 DOI: 10.1016/j.procbio.2025.05.017

Yao Ma , Jincheng Miao , Qiwei Chen , Hongxu Sun , Yanhong Chang , Hui Luo

{"title":"SpyTag/SpyCatcher-mediated SpyRing cyclization improves the operational stability and renaturation efficiency of immobilized enzymes after catalytic inactivation","authors":"Yao Ma , Jincheng Miao , Qiwei Chen , Hongxu Sun , Yanhong Chang , Hui Luo","doi":"10.1016/j.procbio.2025.05.017","DOIUrl":"10.1016/j.procbio.2025.05.017","url":null,"abstract":"<div><div>L-Phenylserine aldolase (LPA), a promising industrial multi-subunit enzyme, catalyzes the condensation of aldehydes with glycine to form β-hydroxy amino acids. This study investigated the effect of SpyTag/SpyCatcher-mediated SpyRing cyclization on operational stability and renaturation after catalytic inactivation. Two immobilized forms of LPA were prepared on a glyoxyl-agarose support (Gx): the native Gx-LPA and its SpyRing-cyclized variant, Gx-SRLPA. The immobilized cyclized enzyme, Gx-SRLPA, exhibited superior operational stability compared to Gx-LPA. A renaturation strategy involving partial unfolding with low concentrations of guanidine hydrochloride (GuHCl) followed by renaturation, proved effective. Gx-SRLPA recovered up to 87.9 % of its activity at 1 M GuHCl and pH 9, exceeding Gx-LPA’s 52.9 % recovery at 1 M GuHCl and pH 7. The presence of dithiothreitol in the unfolding solution and weak alkaline conditions further enhanced Gx-SRLPA’s renaturation. This study highlights the stabilizing effect of the SpyRing structure on enzymes and provides valuable insights into the renaturation of immobilized multi-subunit proteins.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 93-103"},"PeriodicalIF":3.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144166434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient degradation of azo dyes by an engineered myoglobin with a modified heme active site showing high peroxidase activity 具有高过氧化物酶活性的修饰血红素活性位点的工程肌红蛋白有效降解偶氮染料

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-05-27 DOI: 10.1016/j.procbio.2025.05.018

Jing-Jing Liu , Bin-Bin Jiang , Ya-Han Zhu , Shu-Qin Gao , Ying-Wu Lin

{"title":"Efficient degradation of azo dyes by an engineered myoglobin with a modified heme active site showing high peroxidase activity","authors":"Jing-Jing Liu , Bin-Bin Jiang , Ya-Han Zhu , Shu-Qin Gao , Ying-Wu Lin","doi":"10.1016/j.procbio.2025.05.018","DOIUrl":"10.1016/j.procbio.2025.05.018","url":null,"abstract":"<div><div>Azo dyes, widely used in global textiles, persist in wastewater causing significant ecological and health risks, necessitating efficient degradation to protect ecosystems and public health. The development of artificial metalloenzymes has provided advanced biotechnological solutions to address this challenge. This study presents a highly efficient artificial metalloenzyme, F43Y/H64D myoglobin (F43Y/H64D Mb), engineered from sperm whale myoglobin with a modified heme active site by replacement of Phe43 with Tyr and His64 with Asp, respectively, for azo dye degradation. The engineered enzyme achieved degradation efficiencies of approximately 93 %, 93 %, and 90 % for Acid Orange 7, Methyl Orange, and Acid Blue 92, respectively, within 10 seconds. Its overall catalytic efficiency was found to be approximately 3.6 times higher than that of horseradish peroxidase, a widely used natural enzyme. HPLC and ESI-MS analyses proposed degradation pathways, and bacterial toxicity tests confirmed reduced toxicity post-degradation. Furthermore, F43Y/H64D Mb exhibited excellent performance in natural aquatic environments, surpassing many recently reported results in efficiency, reaction speed, and operational simplicity. These findings highlight the feasibility of F43Y/H64D Mb for efficient azo dye decolorization and degradation in wastewater, offering a novel enzymatic tool for sustainable environmental remediation.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 153-163"},"PeriodicalIF":3.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144194426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Platelets as circulatory drug carriers: Emerging potential and its advantages over conventional delivery systems 血小板作为循环药物载体：新兴潜力及其相对于传统给药系统的优势

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-05-26 DOI: 10.1016/j.procbio.2025.05.016

Farzana Naushin , Aryesh Acharjee , Antik Chakraborty , Md Tarique Waheed , Jaydeep Bhattacharya

{"title":"Platelets as circulatory drug carriers: Emerging potential and its advantages over conventional delivery systems","authors":"Farzana Naushin , Aryesh Acharjee , Antik Chakraborty , Md Tarique Waheed , Jaydeep Bhattacharya","doi":"10.1016/j.procbio.2025.05.016","DOIUrl":"10.1016/j.procbio.2025.05.016","url":null,"abstract":"<div><div>Traditional systemic drug delivery methods are often limited by faster clearance rates and high dose requirements. While innovative drug delivery systems, such as liposomes, polymersomes, and solid lipid nanoparticles etc., offer the ability to carry hydrophilic and hydrophobic drugs. But the lack of specificity in the current delivery systems leads to reduced therapeutic efficacy. To overcome these, functionalization with small molecules like peptides, aptamers, and antibodies is done. But they introduce new immunogenicity, stability, and complexity challenges, making these methods costly and less practical. To address these issues, circulatory cell-based drug delivery systems have gained attention as a promising alternative. Unlike red blood cells (RBCs), which have the Rh factor that can complicate, platelets are devoid of this factor and possess inherent advantages. Their natural properties, including non-immunogenicity, hemostasis, pathogen interaction, tissue regeneration, and wound healing, make them highly effective for targeted drug delivery. Furthermore, platelets can form leaky junctions at tumor sites, enhancing localized drug delivery and improving therapeutic outcomes. Thus, platelet-based drug delivery systems are gaining popularity. Moreover, the lower production costs make platelets an efficient and promising tool for a smart drug delivery system. This review highlights the significant role of platelets in advancing targeted drug delivery and their potential to revolutionize therapeutic strategies.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"156 ","pages":"Pages 136-152"},"PeriodicalIF":3.7,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144185011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0