Process Biochemistry最新文献_第8页

Bioconversion of snakeskin shed into bioactive hydrolysate possessing antioxidant and cell proliferative potential 蛇皮蜕皮转化为具有抗氧化和细胞增殖潜能的生物活性水解产物

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2024-11-27 DOI: 10.1016/j.procbio.2024.11.031

Vijan Lal Vikash , Deena Praveena Kumar , Parthasarathy Baskaran Sujiritha , Ganesan Ponesakki , Suresh Kumar Anandasadagopan , Numbi Ramudu Kamini

{"title":"Bioconversion of snakeskin shed into bioactive hydrolysate possessing antioxidant and cell proliferative potential","authors":"Vijan Lal Vikash , Deena Praveena Kumar , Parthasarathy Baskaran Sujiritha , Ganesan Ponesakki , Suresh Kumar Anandasadagopan , Numbi Ramudu Kamini","doi":"10.1016/j.procbio.2024.11.031","DOIUrl":"10.1016/j.procbio.2024.11.031","url":null,"abstract":"<div><div>The underutilization of keratin-rich snakeskin shed (SS) to produce bioactive hydrolysate has not been explored to prevent oxidative stress, limiting its potential in biomedical and nutritional applications. This study aims to generate SS hydrolysate (SSH) through SS degradation using a keratinase-producing <em>Bacillus altitudinis</em> VK-1120. The contents of protein and amino acid of SSH were 13.93 ± 0.48 and 5.57 ± 0.06 mg/mL, respectively, after 96 h. Morphological and chemical changes before and after bacterial degradation were analysed. The SSH contained 16 amino acids, including eight essential amino acids, and demonstrated significant antioxidant activity, with a maximum efficacy of 4.16 ± 0.03 μg/mL for 2,2-diphenyl-1-picrylhydrazyl and 1319.53 ± 8.32 μg/mL for hydroxyl radical scavenging activity, equivalent to L-ascorbic acid. Furthermore, Fe<sup>2+</sup>- chelation and ferric-reducing antioxidant power were found to be 581.33 ± 3.33 μg/mL of EDTA-Na<sub>2</sub> and 193.4 ± 5.83 μM of ferrous (II) sulphate equivalent<sub>,</sub> respectively. Cell viability and migration analysis showcased the proliferation of fibroblast cells with 50, 200, and 500 ng/mL of SSH at 24 h and a reduction in wound area with increasing SSH concentration. Therefore, SS could be utilized as a bioresource for producing both keratinase and antioxidant-rich hydrolysate for identifying biologically active molecules.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"149 ","pages":"Pages 10-21"},"PeriodicalIF":3.7,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142745870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carbon dots derived from dopamine for potent photodynamic bactericidal and antibiofilm application with biocompatibility and low bacterial resistance 由多巴胺衍生的碳点具有生物相容性和低细菌耐药性，可用于有效的光动力杀菌和抗生物膜应用

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2024-11-26 DOI: 10.1016/j.procbio.2024.11.029

Peili Li , Chen Cheng , Boxu Tang , Gan Zhao , Junhua Chen , Yunhe Xu , Da Zhang , Meizhe Yu , Xiang Ke , Zirong Li

{"title":"Carbon dots derived from dopamine for potent photodynamic bactericidal and antibiofilm application with biocompatibility and low bacterial resistance","authors":"Peili Li , Chen Cheng , Boxu Tang , Gan Zhao , Junhua Chen , Yunhe Xu , Da Zhang , Meizhe Yu , Xiang Ke , Zirong Li","doi":"10.1016/j.procbio.2024.11.029","DOIUrl":"10.1016/j.procbio.2024.11.029","url":null,"abstract":"<div><div>Given the ongoing proliferation of bacterial resistance, particularly the rise of “superbugs”, it is imperative to create novel, effective, non-toxic, and non-resistant antibacterial materials and technologies for use in medical settings. Carbon dots derived from dopamine hydrochloride and citric acid (DA-CDs) were effectively produced through a two-step method, displaying photodynamic therapy (PDT) characteristics with low-drug resistance. Only a concentration of 0.125 μg mL<sup>−1</sup> of DA-CDs can effectively break down established <em>S. aureus</em> biofilms when exposed to blue light (450–500 nm), resulting in a biofilm destruction rate of 96.2 %. And after 28 passages, the sensitivity of <em>S. aureus</em> and <em>E. coli</em> to DA-CDs when irradiated with blue light stays the same. The ultra-high negative zeta potential of DA-CDs (-35 mV) and excess intracellular reactive oxygen species (ROS) induced by DA-CDs accounted for their antibacterial and antibiofilm effects. Furthermore, DA-CDs exhibit outstanding biocompatibility without causing harm to red blood cells (<em>RBCs</em>), <em>3T3</em> Cells, bone marrow mesenchymal stem cells (BMSCs) and human umbilical vein endothelial cells (HUVECs). The DA-CDs from this study can effectively kill harmful bacteria and destroy established biofilms using PDT, providing a new alternative to antibiotics and a valuable tool for exploring new treatments for biofilm-related infections.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"148 ","pages":"Pages 222-232"},"PeriodicalIF":3.7,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142745725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antihypertensive peptides from hydrolyzed proteins of Pleurotus spp.: Production, in vitro digestion and identification 侧耳菇水解蛋白抗高血压肽的制备、体外消化和鉴定

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2024-11-23 DOI: 10.1016/j.procbio.2024.11.027

Daniela Fernanda Lima de Carvalho Cavenaghi , Wander Miguel de Barros , Ruann Janser Soares de Castro

{"title":"Antihypertensive peptides from hydrolyzed proteins of Pleurotus spp.: Production, in vitro digestion and identification","authors":"Daniela Fernanda Lima de Carvalho Cavenaghi , Wander Miguel de Barros , Ruann Janser Soares de Castro","doi":"10.1016/j.procbio.2024.11.027","DOIUrl":"10.1016/j.procbio.2024.11.027","url":null,"abstract":"<div><div>This study aimed to investigate <em>in vitro</em> antihypertensive property of protein hydrolysates from <em>Pleurotus</em> spp. mushrooms obtained through enzymatic hydrolysis. Flavourzyme™ 500 L, Alcalase™ 2.4 L and Neutrase™ 0.8 L were used alone and in combination using an experimental design of mixtures. Antihypertensive activity was determined by angiotensin-converting enzyme (ACE) inhibition and the simulated <em>in vitro</em> digestion was performed according to the INFOGEST protocol. Results showed that most of the protein hydrolysates obtained displayed higher ACE inhibitory activity than non-hydrolyzed protein, ranging from 15.76 % and 50.87 % inhibition. The highest ACE inhibition and the lowest TCA soluble protein content (52.09 %) were detected for the protein hydrolysates obtained by using the binary mixture of Flavourzyme™ 500 L and Alcalase™ 2.4 L in equal proportions. Hydrolysis kinetics showed no significant difference in ACE inhibitory activity between 20 and 120 minutes of enzymatic reaction. The fraction with molecular weight between 3 and 5 kDa obtained after ultrafiltration showed the most contribution for ACE inhibitory activity (47 % inhibition). After simulated digestion, the hydrolysates maintained a significant ACE inhibition capacity, indicating the resistance of the peptides to the action of gastrointestinal enzymes. Through proteomic analysis, 26 peptides were identified and the sequences LPILP, IPLLP, PLLPQ and VIQYDPPQ were considered potentially antihypertensive.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"149 ","pages":"Pages 1-9"},"PeriodicalIF":3.7,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142745867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Recent advances in biomarkers detection of various diseases by biosensors derived from optical chromogenic polymeric transducers: A review 利用生物传感器检测各种疾病的生物标记物的最新进展：综述

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2024-11-21 DOI: 10.1016/j.procbio.2024.11.023

Vikrant Sharma, Disha Kapil, Baljit Singh

{"title":"Recent advances in biomarkers detection of various diseases by biosensors derived from optical chromogenic polymeric transducers: A review","authors":"Vikrant Sharma, Disha Kapil, Baljit Singh","doi":"10.1016/j.procbio.2024.11.023","DOIUrl":"10.1016/j.procbio.2024.11.023","url":null,"abstract":"<div><div>The present review article discusses the various sensors for biomarker detection available for multiple diseases with the scope of helping and encouraging researchers to design devices for the effective and early detection of diseases. In general, these sensors are classified into various types based on bio-recognition by detection systems or transducers. However, based on optical transducers, these are further divided into chromogenic, luminogenic, chemiluminescence, and optical fiber. Here in this article, attempts have been made for the analysis of various analytes or biomarkers of various diseases using different polymeric systems under the classification of optical chromogenic transducers. Optical detection of biomarkers in the sample solution is obtained via a biochemical reaction that causes changes in optical properties. Herein, results can be easily analyzed by a color change produced via ligand-analyte interaction, which can be measured to help in the diagnosis of various diseases. There are various types of renal biomarkers, which can be used as an indication of the functioning of the kidney like glucose (GL), uric acid (UA), urea, creatinine (CRN), and albumin (AL). GL is also an important biomarker for diabetes mellitus. For urinary tract infections (UTIs), nitrite is an important biomarker in urine samples. Bilirubin is used as a biomarker for liver damage. Increased chloride concentration in the sweat samples can be used as a biomarker for the disease cystic fibrosis. An elevated cholesterol level inside the body acts as a biomarker for cardiac arrest. Lactate appears to be a powerful predictor of sepsis. This review mainly deals with biochemical reactions for the detection of various biomarkers of diseases. Chemical aspects of the reactions are briefly discussed for their colorimetric evaluation to determine the biomarkers. This article also discusses various polymeric supports available for developing point-of-care testing (POCT) strips.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"148 ","pages":"Pages 191-221"},"PeriodicalIF":3.7,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142721816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Degradation of puffed feather to produce functional biosurfactants by Chitinophaga eiseniae 4 H 嗜壳甲壳虫降解膨化羽毛以产生功能性生物表面活性剂 4 H

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2024-11-19 DOI: 10.1016/j.procbio.2024.11.024

Chen Yuan , Jiaxing Wang , Jin Chuan Wu , Qingxin Li

引用次数: 0

Integration of 2,3-butanediol production and extraction of active components from Chinese herbs by Bacillus licheniformis and evaluation of fermentation products in vitro 地衣芽孢杆菌生产 2,3-丁二醇与提取中草药活性成分的整合及发酵产物的体外评价

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2024-11-19 DOI: 10.1016/j.procbio.2024.11.022

Yanting Xu, Jing Zhuang, Shuo Wang, Jianying Dai, Zhi-Long Xiu

{"title":"Integration of 2,3-butanediol production and extraction of active components from Chinese herbs by Bacillus licheniformis and evaluation of fermentation products in vitro","authors":"Yanting Xu, Jing Zhuang, Shuo Wang, Jianying Dai, Zhi-Long Xiu","doi":"10.1016/j.procbio.2024.11.022","DOIUrl":"10.1016/j.procbio.2024.11.022","url":null,"abstract":"<div><div>The aim of this work was to develop an integrated process of 2,3-butanediol production and extraction of active components from Chinese herbs via fermentation, and evaluate their potential as cosmetic ingredients in vitro. A GRAS strain DL2–2 of <em>Bacillus licheniformis</em> was isolated and the fermentation medium was optimized using the ingredients that could be used in cosmetics. Four types of Chinese herbs were added solely or in combination into the fermentation medium to perform 2,3-butanediol production and active components extraction simultaneously. The release of polysaccharides and flavonoids was promoted, and the antioxidant, whitening and antibacterial abilities were improved. The highest antioxidant and antibacterial activity was obtained from the ferment filtrate of <em>Dendrobium devonianum</em> and <em>Gastrodia elata f. glauca</em>, resulting from the highest contents of flavonoids (0.44 g/L) and polyphenols (0.31 g/L), and 42.2 g/L 2,3-butanediol was also produced. The highest polysaccharide concentration was obtained from the ferment filtrate containing <em>Polygonatum kingianum</em>, which exhibited the highest inhibitory effect on tyrosinase (65 %), similar to that of 1 g/L arbutin. Therefore, the filtrate of 2,3-butanediol-Chinese herbal fermentation broth is promising as an ingredient in the production of natural high-value cosmetics.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"148 ","pages":"Pages 150-156"},"PeriodicalIF":3.7,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142706924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Laccase-mediated synthesis of phloridzin oligomers: An insight into the influence of reaction conditions and evaluation of oligomers’ biological activity 拉克酶介导的氯唑嗪低聚物合成：深入了解反应条件的影响并评估低聚物的生物活性

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2024-11-19 DOI: 10.1016/j.procbio.2024.11.021

Ana Vukoičić , Ana Milivojević , Ljiljana Tolić Stojadinović , Svetlana Grujić , Ljubodrag Vujisić , Vele Tešević , Dejan Bezbradica

{"title":"Laccase-mediated synthesis of phloridzin oligomers: An insight into the influence of reaction conditions and evaluation of oligomers’ biological activity","authors":"Ana Vukoičić , Ana Milivojević , Ljiljana Tolić Stojadinović , Svetlana Grujić , Ljubodrag Vujisić , Vele Tešević , Dejan Bezbradica","doi":"10.1016/j.procbio.2024.11.021","DOIUrl":"10.1016/j.procbio.2024.11.021","url":null,"abstract":"<div><div>The effect of key experimental factors on phloridzin oligomerization catalyzed by <em>Trametes versicolor</em> laccase was investigated, and biological activity of synthesized oligomers was evaluated. Mass spectrometry analysis of the obtained reaction mixture showed the presence of oligomers with the degrees of polymerization two and three with dimer as the major product. The detected masses of the formed oligomers indicated the repetition of phloridzin units in their structure and the loss of two hydrogen atoms during the linkage formation, while NMR analysis of the major dimer confirmed the presence of two phloridzin molecules linked via a C5-C5 bond. The effect of temperature, substrate, and enzyme concentration on phloridzin conversion and products’ yield was examined, and for achieving the highest products yield (4.1 mg/ml), optimal factors were 40 °C temperature, 5 mg/ml phloridzin concentration and 0.5 mg/ml laccase concentration. Synthesized oligomers showed good antioxidant activity compared to phloridzin and very good potential to be used as skin prebiotics since they exhibited inhibitory effect on opportunistic pathogen <em>Staphylococcus aureus</em> and stimulating effect on commensal bacteria <em>Staphylococcus epidermidis</em> in a broad range of tested concentrations. The highest prebiotic capacity was demonstrated at a concentration of 0.0195 mg/ml in <em>S. epidermidis</em> and <em>S. aureus</em> co-culture.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"148 ","pages":"Pages 104-113"},"PeriodicalIF":3.7,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142706922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous saccharification and fermentation of municipal cardboard waste for bioethanol production using immobilized cellulases and xylanases onto Cu-MOF 利用固定在 Cu-MOF 上的纤维素酶和木聚糖酶同时对城市纸板废料进行糖化和发酵以生产生物乙醇

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2024-11-19 DOI: 10.1016/j.procbio.2024.11.025

Tanya Kuthiala , Sudarshan Sahu , Gursharan Singh , Madhu Khatri , Shailendra Kumar Arya

{"title":"Simultaneous saccharification and fermentation of municipal cardboard waste for bioethanol production using immobilized cellulases and xylanases onto Cu-MOF","authors":"Tanya Kuthiala , Sudarshan Sahu , Gursharan Singh , Madhu Khatri , Shailendra Kumar Arya","doi":"10.1016/j.procbio.2024.11.025","DOIUrl":"10.1016/j.procbio.2024.11.025","url":null,"abstract":"<div><div>This study aims to establish a cost-effective and environmentally friendly method for bioethanol production by optimizing enzymatic hydrolysis of cardboard waste, a significant component of municipal solid waste, in combination with fermentation by <em>Saccharomyces cerevisiae</em>. The use of a cellulases and xylanases enzyme blend, sourced from <em>Thermobifida fusca</em> and <em>Bacillus pumilus</em>, respectively, enhances saccharification efficiency. Pre-treatment with 2 % NaOH results in a substantial 66 % saccharification efficiency at a 5 % solid/substrate loading. Further improvement in hydrolysis efficiency is achieved through enzyme immobilization on Cu-BTC-MOFs, which were characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), and Field Emission Scanning Electron Microscopy (FESEM). The immobilized enzyme blend increases saccharification efficiency to 78 %, compared to 66 % for free enzyme systems. Reusability studies demonstrate a gradual reduction in saccharification efficiency across successive cycles, reaching 71.29 % by the twelfth cycle. When applied in a simultaneous saccharification and fermentation (SSF) setup under optimized conditions, the system yields a notable bioethanol production with a yield of 0.55 g/g and a productivity of 0.20 g/L·h. This study offers potential applications in sustainable bioethanol production and waste valorization by enhancing enzymatic hydrolysis and fermentation of municipal cardboard waste. The approach demonstrates promise for scaling bioenergy production and contributes to addressing waste management challenges, supporting green energy initiatives.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"148 ","pages":"Pages 114-123"},"PeriodicalIF":3.7,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142707010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Linguistics of microbiota – Quorum sensing in anaerobic digestion, biochemistry, characteristics, and perspectives of future research – A mini-review 微生物群的语言学 - 厌氧消化中的法定人数感应、生物化学、特点和未来研究展望 - 小综述

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2024-11-19 DOI: 10.1016/j.procbio.2024.11.026

Michał Kozłowski , Krzysztof Marycz , Andrzej Białowiec

{"title":"Linguistics of microbiota – Quorum sensing in anaerobic digestion, biochemistry, characteristics, and perspectives of future research – A mini-review","authors":"Michał Kozłowski , Krzysztof Marycz , Andrzej Białowiec","doi":"10.1016/j.procbio.2024.11.026","DOIUrl":"10.1016/j.procbio.2024.11.026","url":null,"abstract":"<div><div>This manuscript discusses the mechanism of quorum sensing, provides a brief description of the phenomenon, biochemistry properties, and quorum quenching mechanism, and provides a perspective for future research with some implications regarding its use in industry. Particular emphasis was placed on using these signal molecules in anaerobic digestion. A proper understanding of the processes occurring during methane fermentation could be crucial to in the prospects of increasing biogas production. The types of signaling molecules vary between microorganisms, and the way they are synthesized or secreted (active or passive) differs. This type of communication could induce biofilm formation, bioluminescence, metabolite synthesis, motility, and conjugation. Appropriate knowledge and understanding of the operation of signaling molecules might be essential to increasing the efficiency of anaerobic digestion processes. The phenomenon of quorum sensing itself can be successfully used in medicine, renewable energy (biogas plants), or the chemical industry.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"148 ","pages":"Pages 183-190"},"PeriodicalIF":3.7,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142706921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing riboflavin production by modulating the expression of the rib operon in Bacillus subtilis 通过调节枯草芽孢杆菌核糖操作子的表达提高核黄素产量

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2024-11-17 DOI: 10.1016/j.procbio.2024.11.020

Mengyu Yao , Miaomiao Xia , Jinlong Li , Huina Dong , Liping Du , Dawei Zhang

{"title":"Enhancing riboflavin production by modulating the expression of the rib operon in Bacillus subtilis","authors":"Mengyu Yao , Miaomiao Xia , Jinlong Li , Huina Dong , Liping Du , Dawei Zhang","doi":"10.1016/j.procbio.2024.11.020","DOIUrl":"10.1016/j.procbio.2024.11.020","url":null,"abstract":"<div><div>Riboflavin is an essential vitamin for humans and animals, with its annual demand experiencing a consistent upward trajectory. In recent years, newly constructed riboflavin producing strains are difficult to reach the level of industrial strains. Therefore, developing competitive strains is crucial for advancing the riboflavin industry. The expression level of the <em>rib</em> operon is strongly correlated with riboflavin biosynthesis. The strain S12, which overexpressed the <em>rib</em> operon of <em>B. subtilis</em> using plasmids with approximately 8 copies, achieved a 53.9 % increase in riboflavin biosynthesis during shake-flask fermentation. However, the strain cannot grow normally in a 7.5-L bioreactor. The expression of <em>rib</em> operon may have competed with cell growth for GTP or produced toxic intermediate ArP. Therefore, the strain S16 replaced <em>ribA</em> in <em>rib</em> operon with a monofunctional DHBP synthase, increased 20 % of riboflavicn production in a 7.5-L bioreactor. Furthermore, the strain S24, which overexpressed the <em>rib</em> operon using the phase-dependent promoter P<sub><em>yqgZ</em></sub>, attained a riboflavin production of 29 g/L in a 7.5-L bioreactor, achieving the highest reported riboflavin production to date. This study reports for the first time using the phase-dependent promoter for overexpression of <em>rib</em> operon, effectively regulated the relationship between cell growth and riboflavin production.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"148 ","pages":"Pages 139-149"},"PeriodicalIF":3.7,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142706926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0