Process Biochemistry最新文献_第8页

Performance assessment of a bio-electro-Fenton system for azo-dye removal from synthetic wastewater 生物电fenton系统去除合成废水中偶氮染料的性能评价

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-04-22 DOI: 10.1016/j.procbio.2025.04.017

Carlos Eduardo Lach , Daniele Damasceno Silveira , Tiago José Belli , Flávio Rubens Lapolli , María Ángeles Lobo-Recio

{"title":"Performance assessment of a bio-electro-Fenton system for azo-dye removal from synthetic wastewater","authors":"Carlos Eduardo Lach , Daniele Damasceno Silveira , Tiago José Belli , Flávio Rubens Lapolli , María Ángeles Lobo-Recio","doi":"10.1016/j.procbio.2025.04.017","DOIUrl":"10.1016/j.procbio.2025.04.017","url":null,"abstract":"<div><div>This investigation aimed to evaluate the application of a bio-electro-Fenton (BEF) system for Remazol brilliant violet – 5R (RBV-5R) removal. Three experimental runs were conducted under varied conditions using RBV-5R concentrations of 5, 10, and 20 mg L<sup>−1</sup>, external resistances (R<sub>ext</sub>) of 1000, 100, and 10 Ω, and a hydraulic retention time (HRT) of 12 h. At 20 mg L<sup>−1</sup> RBV-5R and 10 Ω, removal efficiencies for color, aromatic groups, and chemical oxygen demand (COD) reached 95.5 ± 0.25 %, 73.6 ± 0.4 %, and 82.4 ± 0.3 %, respectively. A residual hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) concentration of 12.25 ± 0.17 mg L<sup>−1</sup> was measured at a maximum power density of 69.4 mW m<sup>−2</sup>, with Coulomb and Faraday efficiencies of 7.2 ± 0.4 % and 5.81 ± 0.1 %. Phytotoxicity assays showed that the germination index (GI) for <em>L. sativa</em> and <em>R. sativus</em> increased from approximately 6 – 39 % after 12 h. A strong positive correlation (r = 0.926, p < 0.01) between residual H<sub>2</sub>O<sub>2</sub> and the microbial community suggests that the anodic biofilm may enhance H<sub>2</sub>O<sub>2</sub> generation at the cathode for the Fenton reaction. These results support BEF systems as a promising technology for azo dye removal without external energy or H<sub>2</sub>O<sub>2</sub> addition.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"154 ","pages":"Pages 157-171"},"PeriodicalIF":3.7,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143883139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing plant growth, polyphenols accumulation, and bioactivity of Mentha rotundifolia L. by dual application of exogenous indole acetic acid and adenine-like cytokinin 通过外源吲哚乙酸和腺嘌呤样细胞分裂素的双重应用促进薄荷的植物生长、多酚积累和生物活性

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-04-18 DOI: 10.1016/j.procbio.2025.04.006

Hadjer Kecis , Fatiha Mekircha , Lynda Gali , Mohamed Bagues , Yahia Abdelouhab , Kamel Nagaz

{"title":"Enhancing plant growth, polyphenols accumulation, and bioactivity of Mentha rotundifolia L. by dual application of exogenous indole acetic acid and adenine-like cytokinin","authors":"Hadjer Kecis , Fatiha Mekircha , Lynda Gali , Mohamed Bagues , Yahia Abdelouhab , Kamel Nagaz","doi":"10.1016/j.procbio.2025.04.006","DOIUrl":"10.1016/j.procbio.2025.04.006","url":null,"abstract":"<div><div>The present work explores the effects of combined treatments with two phytohormones, adenine-type cytokinin (AD) and indole acetic acid (IAA), on the growth, phenolic content, and biological activities of <em>Mentha rotundifolia</em> L. Four combinations of IAA and AD, which consist of 10 mg/L and/or 20 mg/L (T1, T2, T3, and T4), were applied to the plant and parameters including fresh weight, dry weight, polyphenols contents, HPLC profile, and the activity of the extracts were determined and compared to a control (untreated plants). The application of phytohormones significantly improved plant fresh and dry weights. Total phenolic contents were also increased for all treatments (a maximum of 235.7 µg GAE/mL in the aerial parts and 252.69 µg GAE /mL in the roots were recorded with the treatment T4), while flavonoids and flavonols contents varied according to the type of treatment and the plant organ. Treatments T1 and T4 induced the most significant increase in the main phenolic acids of the aerial part (quinic acid, rosmarinic acid and salvialonic acid), while individual flavonoids were mainly promoted by the treatment T4 in both organs. The antioxidant and enzyme inhibitory effects of the extracts were also enhanced for the treated samples. Naringin, rosmarinic acid and salvianolic acid B were suggested as the major phenolics contributing to AChE and α-glucosidase inhibition exhibiting low binding energies (−9.8 and −10.5, − 8.9 and −8.4, − 8.8 and − 9.8 respectively) and their contents were highest in the treatments giving the strongest inhibitory effect. The various discoveries affirm the possible use of IAA/AD in combination to induce the development and accumulation of polyphenols in <em>Mentha rotundifolia</em> L. and therefore the improvement of its pharmacological effects.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"155 ","pages":"Pages 37-50"},"PeriodicalIF":3.7,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143855800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytochemical analysis and biological activities of Leiotulus aureus extracts: Antioxidant, anticholinesterase, antidiabetic, and antidepressant properties 金银花提取物的植物化学分析和生物活性：抗氧化、抗胆碱酯酶、抗糖尿病和抗抑郁的特性

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-04-17 DOI: 10.1016/j.procbio.2025.04.014

Elife Kaya

{"title":"Phytochemical analysis and biological activities of Leiotulus aureus extracts: Antioxidant, anticholinesterase, antidiabetic, and antidepressant properties","authors":"Elife Kaya","doi":"10.1016/j.procbio.2025.04.014","DOIUrl":"10.1016/j.procbio.2025.04.014","url":null,"abstract":"<div><div>This study aimed to evaluate the antioxidant properties, phytochemical contents (LC-MS/MS), and inhibitory effects of <em>Leiotulus aureus</em> extracts on enzymes related to Alzheimer's disease, diabetes, and depression. This is the first <em>L. aureus</em> enzyme inhibition study<em>.</em> In the study, the ethanol extract exhibited the strongest antioxidant effect scavenging DPPH (IC<sub>50</sub>: 43.76 mg/mL) and ABTS (IC<sub>50</sub>: 22.41 mg/mL) radical and Fe<sup>+3</sup>-Fe<sup>+2</sup> reducing capacity. In the LC-MS/MS analysis, fumaric acid (1.262 mg/g) and quinic acid (1.074 mg/g) were identified as major components in the ethanol extract, which also exhibited high phenolic (32.69 mg GAE/g) and flavonoid (20.19 mg QE/g) contents. In contrast genistin (0.047 mg/g) was identified as the main component in the dichloromethane: methanol extract. The ethanol extract exhibited the highest inhibitory effect against AChE and BChE, while the dichloromethane: methanol extract showed the strongest effect against α-glycosidase and α-amylase. The inhibitory effect of the dichloromethane extract (IC<sub>50</sub>: 6.54 mg/mL) on the MAO-B enzyme was found to be very close to that of the standard inhibitor pargyline HCl (IC<sub>50</sub>: 5.06 mg/mL). These findings indicate that the plant may possess multifunctional pharmacological properties, such as antioxidant, antidiabetic, and antidepressant effects, and point to a potential natural agent, especially for neuropsychiatric disorders.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"154 ","pages":"Pages 75-83"},"PeriodicalIF":3.7,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143847856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of culture conditions of Trichosporon oleaginosus DSM11815 for enhancement of growth and lipid production in sugarcane bagasse hydrolysate as a substrate 以甘蔗渣水解物为底物促进生长和产脂的油脂毛孢菌DSM11815培养条件的评价

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-04-17 DOI: 10.1016/j.procbio.2025.04.015

Yuanping Fang , Pingzhong Feng , Li Wang , Lei Qin , Zhongming Wang , Shunni Zhu , Wen Wang , Wei Qi , Shian Wang , Yong Fan

{"title":"Evaluation of culture conditions of Trichosporon oleaginosus DSM11815 for enhancement of growth and lipid production in sugarcane bagasse hydrolysate as a substrate","authors":"Yuanping Fang , Pingzhong Feng , Li Wang , Lei Qin , Zhongming Wang , Shunni Zhu , Wen Wang , Wei Qi , Shian Wang , Yong Fan","doi":"10.1016/j.procbio.2025.04.015","DOIUrl":"10.1016/j.procbio.2025.04.015","url":null,"abstract":"<div><div>In this study, the growth and lipid production of <em>Trichosporon oleaginosus</em> DSM11815 were optimized, and the potential of sugarcane bagasse hydrolysate as an alternative carbon source for culturing the oleaginous yeast was evaluated. The fermentation process in the potato glucose agar (PDA) medium was optimized through single-factor experiments, including initial total sugar concentration, nitrogen source type, carbon nitrogen ratio (C/N ratio), KH<sub>2</sub>PO<sub>4</sub> concentration, fed-batch period, and composition of the fed-batch solution. In the optimized PDA medium, which contained 20 g/L glucose, 7.5 g/L xylose, 0.51 g/L urea, 0.2 g/L KH<sub>2</sub>PO<sub>4</sub>, the two-stage culture through fed-batch with <em>T. oleaginosus</em> DSM11815 led to the production of dry weight of 28.2 g/L with the lipid content of 41.1 %. In comparison to the groups of the optimized PDA medium and sugarcane bagasse hydrolysate, the group that utilized sugarcane bagasse hydrolysate to replace half of the optimized PDA medium obtained the highest biomass (39.4 g/L), the highest lipid content (50.00 %), the highest glucose consumption (100 %) and the highest xylose consumption (48 %). The fatty acid composition of all groups mainly consists of long-chain fatty acids of 16 and 18 carbons. These results supported for the utilization of sugarcane bagasse hydrolysate as a cost-effective alternative carbon source.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"155 ","pages":"Pages 23-36"},"PeriodicalIF":3.7,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143855799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure-guided discovery of natural α-glucosidase inhibitors from food sources for blood sugar regulation 结构引导下从食物中发现用于血糖调节的天然α-葡萄糖苷酶抑制剂

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-04-17 DOI: 10.1016/j.procbio.2025.04.011

Bo-Hao Liu , Hong-Fu Zhao , Zi-Ying Zhao , Bo-Kang Yu , Ying-Hua Zhang , Zhi-Shen Mu

{"title":"Structure-guided discovery of natural α-glucosidase inhibitors from food sources for blood sugar regulation","authors":"Bo-Hao Liu , Hong-Fu Zhao , Zi-Ying Zhao , Bo-Kang Yu , Ying-Hua Zhang , Zhi-Shen Mu","doi":"10.1016/j.procbio.2025.04.011","DOIUrl":"10.1016/j.procbio.2025.04.011","url":null,"abstract":"<div><div>Type II diabetes mellitus (T2DM) has become a global health crisis, with prevalence rates escalating dramatically over the past three decades. α-glucosidase inhibitors have emerged as effective substances for glycemic control. In this study, a library of α-glucosidase inhibitors with definite inhibitory action was constructed, and models for the quantitative structure-activity relationship (QSAR) were developed by combining four descriptors—MOE, ChemoPy, Mordred, and RDKit, and three machine learning algorithms—RF, LDA, and SVM. Molecular docking revealed that hydrogen bonding and van der Waals interactions critically stabilize inhibitor-enzyme binding, while molecular dynamics (MD) simulations (100 ns) confirmed the structural stability of the complexes. From FooDB, amentoflavone emerged as a potent natural inhibitor with an IC<sub>50</sub> of 10.77 ± 1.03 μM. Notably, amentoflavone’s minimal side effects and natural origin highlight its potential as a safer therapeutic alternative. Our multi-faceted approach combining QSAR, molecular docking, MD dynamics, and in vitro validation, provides a rapid and cost-effective framework for discovering functional hypoglycemic compounds from food sources, advancing both mechanistic understanding and practical applications in diabetes management.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"155 ","pages":"Pages 1-12"},"PeriodicalIF":3.7,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143845217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrating waste management with biopolymer innovation: A new frontier in xanthan gum production 整合废物管理与生物聚合物创新：黄原胶生产的新前沿

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-04-16 DOI: 10.1016/j.procbio.2025.04.012

Richard Vincent Asase, Tatiana Vladimirovna Glukhareva

{"title":"Integrating waste management with biopolymer innovation: A new frontier in xanthan gum production","authors":"Richard Vincent Asase, Tatiana Vladimirovna Glukhareva","doi":"10.1016/j.procbio.2025.04.012","DOIUrl":"10.1016/j.procbio.2025.04.012","url":null,"abstract":"<div><div>Xanthan gum, is a biopolymer with extensive applications across various industries due to its versatile rheological properties. A critical challenge in xanthan production is the high cost, primarily driven by the expense of glucose, which accounts for about 70 % of the cost of production materials as the primary carbon source. Consequently, there is a growing interest in the search for cost-effective and environmentally friendly alternatives. Nevertheless, xanthan produced from these alternative raw materials comes with various technical challenges. This review therefore collates the recent findings in the use of food and agro-industrial waste as alternative carbon sources, offering a novel and sustainable solution to wastes management and cost challenge. For the first time, interdisciplinary connections between waste valorization, fermentation optimization, and changes in properties are revealed, showcasing how waste substrates can influence the properties of the obtained xanthan gum compared to gum produced by classical technology. Revealed yields and thermal stability of such gums match commercial standards, while their structure and functional properties may vary, indicating the need for quality control. Also, substituting refined sugars with waste reduced raw material costs by 60–70 %. This approach addresses waste management issues and greener production strategies in the xanthan industry.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"154 ","pages":"Pages 84-98"},"PeriodicalIF":3.7,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143847855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of environmentally friendly plasticizers derived from soybean oils using biochemical (lipases) and chemical catalysts – Performance in flexible PVC films 使用生化（脂肪酶）和化学催化剂从大豆油中提取的环保型增塑剂的生产。柔性PVC薄膜的性能

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-04-15 DOI: 10.1016/j.procbio.2025.04.013

Guilherme J. Sabi , Ana L.A. Simões , Raphael A.B. Gomes , Washington A. da Silva , Luiz F. Gorup , Adriano A. Mendes

{"title":"Production of environmentally friendly plasticizers derived from soybean oils using biochemical (lipases) and chemical catalysts – Performance in flexible PVC films","authors":"Guilherme J. Sabi , Ana L.A. Simões , Raphael A.B. Gomes , Washington A. da Silva , Luiz F. Gorup , Adriano A. Mendes","doi":"10.1016/j.procbio.2025.04.013","DOIUrl":"10.1016/j.procbio.2025.04.013","url":null,"abstract":"<div><div>A new and sustainable approach to produce biobased plasticizers from renewable raw materials such as refined soybean oil (RSBO) and used soybean cooking oil (USCO) for flexible PVC film applications has been demonstrated. Decyl esters have been produced via a two-step enzymatic process (hydroesterification), which involves the complete conversion of these oils into free fatty acids (FFAs) via hydrolysis, followed by the esterification of the resulting FFAs with decanol in solvent-free systems. A third step has been utilized to incorporate epoxy groups via an in situ epoxidation process. The properties of PVC films containing these biobased decyl esters were investigated and compared with those of plasticized film using petroleum-derived dioctyl phthalate (DOP) and pure PVC films. The introduction of polar groups (ester and/or epoxy) into the plasticizer structures, confirmed via standard methods and NMR analysis, enhances their interaction and dispersion within the PVC network. The incorporation of these biobased plasticizers, particularly the epoxidized decyl esters derived from waste oil (EDE-USCO), provided the PVC films with improved thermal, mechanical, optical, and migration stability properties relative to those prepared with DOP. The potential of these biobased plasticizers, especially EDE-USCO, for producing flexible PVC films with favorable properties has been established.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"154 ","pages":"Pages 63-74"},"PeriodicalIF":3.7,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143838899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analysis of anti-solvent and emulsification techniques for the synthesis of quercetin-epigallocatechin gallate nanowires: Characterization and biomedical applications 槲皮素-表没食子儿茶素没食子酸酯纳米线合成的抗溶剂和乳化工艺对比分析：表征及其生物医学应用

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-04-15 DOI: 10.1016/j.procbio.2025.04.010

Ola M. El-Borady , Mohamed Moustafa Abdelaal

{"title":"Comparative analysis of anti-solvent and emulsification techniques for the synthesis of quercetin-epigallocatechin gallate nanowires: Characterization and biomedical applications","authors":"Ola M. El-Borady , Mohamed Moustafa Abdelaal","doi":"10.1016/j.procbio.2025.04.010","DOIUrl":"10.1016/j.procbio.2025.04.010","url":null,"abstract":"<div><div>The current study was based on the idea of accomplishing the advantages of two polyphenols, quercetin (QT) and Epigallocatechin Gallate (EGCG), by synthesizing a nano-combination model using two different methods: emulsification (Mix1) and the anti-solvent method (Mix2). Morphological studies of QT, EGCG, Mix1, and Mix2 were performed using transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), Brunauer-Emmett- Teller (BET) surface area analysis, and Fourier-transform infrared spectroscopy (FTIR). Antimicrobial efficacy and Minimum Inhibitory Concentration (MIC) were tested against <em>Salmonella typhi</em> and nine other human pathogens. Antioxidant activity was also evaluated. The results showed the formation of nanowires using both methods. Mix1 exhibited agglomerated and adhered particles, whereas Mix2 exhibited rougher morphology. Maximum absorbance for both Mix1 and Mix2 was observed at 374 nm. FT-IR analysis confirmed that QT and EGCG were physically attracted to each other. Mix1 and Mix2 exhibit enhanced synergistic antimicrobial and antioxidant properties compared with the original polyphenols, which may enhance their bioavailability.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"155 ","pages":"Pages 51-59"},"PeriodicalIF":3.7,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143869901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a versatile and high-loading capacity yeast surface assembly system via SpyC/SpyT conjugation 利用SpyC/SpyT偶联技术开发一种多功能高负荷酵母表面组装系统

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-04-12 DOI: 10.1016/j.procbio.2025.04.004

Ke Xu , Hailin Liu , Chao Du , Faying Zhang , Wenya Wang , Guimin Zhang

{"title":"Development of a versatile and high-loading capacity yeast surface assembly system via SpyC/SpyT conjugation","authors":"Ke Xu , Hailin Liu , Chao Du , Faying Zhang , Wenya Wang , Guimin Zhang","doi":"10.1016/j.procbio.2025.04.004","DOIUrl":"10.1016/j.procbio.2025.04.004","url":null,"abstract":"<div><div>The yeast surface display (YSD) system for enzyme immobilization has a wide range of applications and is of great value in various studies. However, despite the many advantages of the YSD system, the amount of proteins it can display is limited, which restricts its potential applications. To enhance the applicability of the YSD system, we introduced a highly efficient and stable conjugation tool (SpyCatcher-SpyTag (SpyC-SpyT)) into the YSD system and developed a <em>Saccharomyces cerevisiae</em> surface assembly (SSA) system with high loading capacity. The SpyC-SpyT can spontaneously form molecular isopeptide bonds, and the target proteins can be assembled in the SSA system within 5 min. By increasing the copy of SpyC and integrating the expression fragment into the rDNA region, we successfully screened a genetically stable and high-copy strain that could assemble ∼7.8 × 10<sup>6</sup> SpyT-eGFP molecular count/cell. The organophosphate hydrolase immobilized by the SSA system not only has the similar enzyme activity as the free enzyme, but also has improved thermal stability and enhanced tolerance to acidic and alkaline environments. Further, we found that the immobilized enzyme still retained 77.7 % of its enzyme activity after 6 cycles. In conclusion, we successfully constructed a novel, efficient and stable SSA system for the development of the YSD system.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"155 ","pages":"Pages 13-22"},"PeriodicalIF":3.7,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143848702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Covalent immobilization of glucoamylase on 3D-printed poly(lactic acid) carriers for starch hydrolysis in stirred tank reactor 在 3D 打印聚（乳酸）载体上共价固定葡萄糖淀粉酶，用于搅拌罐反应器中的淀粉水解

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-04-11 DOI: 10.1016/j.procbio.2025.04.003

David Spressão de Lima Junior , Ilana Sessak , Daniela Remonatto , Julián Paul Martínez Galán , Murilo Daniel de Mello Innocentini , Ariela Veloso de Paula , Daniela Alonso Bocchini

{"title":"Covalent immobilization of glucoamylase on 3D-printed poly(lactic acid) carriers for starch hydrolysis in stirred tank reactor","authors":"David Spressão de Lima Junior , Ilana Sessak , Daniela Remonatto , Julián Paul Martínez Galán , Murilo Daniel de Mello Innocentini , Ariela Veloso de Paula , Daniela Alonso Bocchini","doi":"10.1016/j.procbio.2025.04.003","DOIUrl":"10.1016/j.procbio.2025.04.003","url":null,"abstract":"<div><div>This study presents a novel 3D-printed poly(lactic acid) (PLA) carrier for the covalent immobilization of a commercial glucoamylase. Enzyme carriers were functionalized by aminolysis with ethylenediamine (EDA) and activated with glutaraldehyde. Since covalent immobilization using glutaraldehyde as activating agent involves the formation of imine bonds which are inherently unstable, the use of borohydride as a reducing agent to stabilize these imine bonds after the functionalization step was considered. The highest enzymatic activity (13.68 U g<sup>−1</sup> carrier) was obtained when immobilization was performed at pH 10 using NaBH<sub>4</sub> (immobilization yield of 32.80 % ± 0.51 %). Optimal activity conditions were pH 4.92 and 50 °C for the soluble enzyme and pH 5.5 and 50 °C for the immobilized enzyme. The operational stability of immobilized glucoamylase was evaluated for 10 consecutive reaction cycles (of 10 minutes each) and the enzyme maintained 65.19 % of its original activity at the end of the third cycle. Starch saccharification in a stirred tank reactor by immobilized glucoamylase reached 95 % conversion after 12 h and 74 % conversion after 12 h in the first reuse cycle. These findings demonstrate the potential of PLA for glucoamylase immobilization, offering promising prospects for efficient and sustainable starch hydrolysis in industrial enzymatic processes.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"154 ","pages":"Pages 52-62"},"PeriodicalIF":3.7,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143834315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0