Evaluation of culture conditions of Trichosporon oleaginosus DSM11815 for enhancement of growth and lipid production in sugarcane bagasse hydrolysate as a substrate

Abstract

In this study, the growth and lipid production of Trichosporon oleaginosus DSM11815 were optimized, and the potential of sugarcane bagasse hydrolysate as an alternative carbon source for culturing the oleaginous yeast was evaluated. The fermentation process in the potato glucose agar (PDA) medium was optimized through single-factor experiments, including initial total sugar concentration, nitrogen source type, carbon nitrogen ratio (C/N ratio), KH₂PO₄ concentration, fed-batch period, and composition of the fed-batch solution. In the optimized PDA medium, which contained 20 g/L glucose, 7.5 g/L xylose, 0.51 g/L urea, 0.2 g/L KH₂PO₄, the two-stage culture through fed-batch with T. oleaginosus DSM11815 led to the production of dry weight of 28.2 g/L with the lipid content of 41.1 %. In comparison to the groups of the optimized PDA medium and sugarcane bagasse hydrolysate, the group that utilized sugarcane bagasse hydrolysate to replace half of the optimized PDA medium obtained the highest biomass (39.4 g/L), the highest lipid content (50.00 %), the highest glucose consumption (100 %) and the highest xylose consumption (48 %). The fatty acid composition of all groups mainly consists of long-chain fatty acids of 16 and 18 carbons. These results supported for the utilization of sugarcane bagasse hydrolysate as a cost-effective alternative carbon source.