Process Biochemistry最新文献_第9页

Efficient kinetic resolution of D,L-phosphinothricin using an aminotransferase-mediated cascade 利用转氨酶介导的级联高效动力学分解D， l -膦-丙氨酸

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-02-01 DOI: 10.1016/j.procbio.2024.12.021

Puhong Yi , Yue Xu , Hanlin Liu , Yuhua Hao , Mengdan Liu , Zhiqiang Liu , Yaping Xue , Liqun Jin , Yuguo Zheng

{"title":"Efficient kinetic resolution of D,L-phosphinothricin using an aminotransferase-mediated cascade","authors":"Puhong Yi , Yue Xu , Hanlin Liu , Yuhua Hao , Mengdan Liu , Zhiqiang Liu , Yaping Xue , Liqun Jin , Yuguo Zheng","doi":"10.1016/j.procbio.2024.12.021","DOIUrl":"10.1016/j.procbio.2024.12.021","url":null,"abstract":"<div><div>2-oxo-4-[(hydroxy)(methyl)phosphinoyl]butyric acid (PPO) is a valuable prochiral keto acid for the asymmetric synthesis of the important herbicide L-phosphinothricin (L-PPT). Developing an efficient biocatalytic pathway to meet the increasing demand for PPO is highly attractive. In this study, a robust D-amino acid aminotransferase from <em>Cytobacillus firmus</em> (<em>Cf</em>DAAT) was screened, which exhibited excellent tolerability to D,L-PPT and showed remarkable specific activity and affinity toward pyruvate (61.9 U/mg, Km = 7.4 mM). A designed co-substrate regeneration system was then introduced to shift the reaction equilibrium forward by coupling with D-amino acid oxidase (DAAO) and catalase (CAT). An efficient whole-cell biocatalyst was obtained by fine-tuning RBS intensity to coordinate the co-expression of the three enzymes. Moreover, the optimum conditions to promote overall conversion efficiency were identified by response surface methodology. Consequently, with only a catalytic amount of pyruvate (3.33 mol%) added in a 1 L reaction system, 200 mM D-PPT (400 mM D,L-PPT) was completely converted in 10 h, resulting in a PPO productivity of 3.33 g⋅L<sup>−1</sup>⋅h<sup>−1</sup>, and a notable conversion rate of > 99 % at 300 mM D-PPT in 16 h. It demonstrated a considerable catalytic level and provided a competitive route for industrial PPO production from D,L-PPT.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"149 ","pages":"Pages 295-305"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143139497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Separation of C-Phycocyanin from Spirulina sp. using the coupled processes of an ion-exchange resin-packed column and ultrafiltration module 离子交换树脂填充柱与超滤模块耦合分离螺旋藻中c -藻蓝蛋白

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-02-01 DOI: 10.1016/j.procbio.2024.12.020

Takanori Hidane , Haruka Miyoshi , Mikihide Demura , Shintaro Morisada , Keisuke Ohto , Hidetaka Kawakita

{"title":"Separation of C-Phycocyanin from Spirulina sp. using the coupled processes of an ion-exchange resin-packed column and ultrafiltration module","authors":"Takanori Hidane , Haruka Miyoshi , Mikihide Demura , Shintaro Morisada , Keisuke Ohto , Hidetaka Kawakita","doi":"10.1016/j.procbio.2024.12.020","DOIUrl":"10.1016/j.procbio.2024.12.020","url":null,"abstract":"<div><div>C-Phycocyanin (CPC) is a pigment protein produced by cyanobacteria and is commonly used as a food coloring and in cosmetics. For the separation and concentration of CPC, a novel combined process of chromatographic column and ultrafiltration module is proposed. The unit of the column and ultrafiltration module are described mathematically to calculate the combined units as a process. The ion-exchange resin-packed column separated CPC with a purity of 2.0 or higher at a rate of 15 mL per cycle. Ultrafiltration module (MWCO 30 kDa) was operated in desalting mode (diafiltration: DF) and concentration mode (ultrafiltration: UF). DF reduced the salt concentration from an initial concentration of 260 mM to 40 mM, with no proteins leaking from the module. UF increased CPC concentration from 0.82 g/L to 1.48 g/L. Parameters were determined using a mathematical model to simulate the combined process of ion-exchange resin-packed column and ultrafiltration module, and conditions (permeation velocity and time of ultrafiltration) were determined to achieve the desired product characteristics. Different from separation unit operation, combined process of a resin-packed column and ultrafiltration module was capable of separating CPC with approximately twice the productivity of other methods.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"149 ","pages":"Pages 260-269"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143139501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zein-quercetin covalent nanoparticles encapsulating oregano essential oil: Improved stability, antioxidant, and antibacterial properties 玉米素-槲皮素共价纳米颗粒包封牛至精油：提高稳定性，抗氧化和抗菌性能

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-02-01 DOI: 10.1016/j.procbio.2024.12.015

Lingyu Yin, Yuhang Cao, Meihui Wang, Baohua Kong, Qian Liu, Hui Wang, Hao Wang

{"title":"Zein-quercetin covalent nanoparticles encapsulating oregano essential oil: Improved stability, antioxidant, and antibacterial properties","authors":"Lingyu Yin, Yuhang Cao, Meihui Wang, Baohua Kong, Qian Liu, Hui Wang, Hao Wang","doi":"10.1016/j.procbio.2024.12.015","DOIUrl":"10.1016/j.procbio.2024.12.015","url":null,"abstract":"<div><div>The zein-quercetin covalent complexes were prepared by alkali treatment (A-ZEIN-Q) and free radical grafting (R-ZEIN-Q) and subsequently followed by encapsulation of oregano essential oil (OEO), and alkali grafted zein-quercetin covalent nanoparticles (A-ZQNPs-OEO) and free radical grafted zein-quercetin covalent nanoparticles (R-ZQNPs-OEO) were synthesized respectively. Additionally, the particle size, ζ-potential, microstructure, encapsulation efficiency, release behaviour, surface hydrophobicity, stability, antioxidant activity, and antibacterial activity of these nanoparticles were characterized. The results of infrared spectroscopy showed that a covalent bond was formed between zein and quercetin, the particle size of the nanoparticles decreased, and the degree of densification was higher than that of zein nanoparticles. Unlike the aggregated zein nanoparticles, the zein-quercetin nanoparticles were dispersed more uniformly. In addition, the encapsulation efficiency of OEO in A-ZQNPs-OEO and R-ZQNPs-OEO increased significantly from 45.11 % to 73.42 % and 70.90 % to that of OEO in single zein nanoparticles, respectively. Among the four nanoparticles, A-ZQNPs-OEO shows the highest stability, while the OEO-encapsulated quercetin covalent nanoparticles show better-sustained release performance, lower surface hydrophobicity, and excellent antioxidant and antibacterial activities. Overall, the synthesized OEO encapsulated zein-quercetin covalent nanoparticles possess the ability to be applied in food preservation and other applications.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"149 ","pages":"Pages 248-259"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143139492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nutritional value and chemical properties of drone milk as a source of probiotics and evaluation of antioxidant effects on reproductive structures by in vitro test 蜂乳作为益生菌来源的营养价值、化学性质及体外抗氧化作用评价

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-02-01 DOI: 10.1016/j.procbio.2024.12.007

Miroslava Kačániová , Mária Škultétyová , Eva Tvrdá , Filip Benko , Michal Ďuračka , Natália Čmiková , Eva Ivanišová , Jaroslav Havlík , Grzegorz Zaguła , Angel Antonio Carbonell Barrachina , Stefania Garzoli

{"title":"Nutritional value and chemical properties of drone milk as a source of probiotics and evaluation of antioxidant effects on reproductive structures by in vitro test","authors":"Miroslava Kačániová , Mária Škultétyová , Eva Tvrdá , Filip Benko , Michal Ďuračka , Natália Čmiková , Eva Ivanišová , Jaroslav Havlík , Grzegorz Zaguła , Angel Antonio Carbonell Barrachina , Stefania Garzoli","doi":"10.1016/j.procbio.2024.12.007","DOIUrl":"10.1016/j.procbio.2024.12.007","url":null,"abstract":"<div><div>The objective of this study was to compare the probiotic bacteria present in drone milk (DM) and drone larvae (DL). Furthermore, the study aimed to investigate the antibacterial capacity against <em>Staphylococcus</em> and <em>Streptococcus</em> species isolated from animal semen. The <em>in vitro</em> effects of DM and DL on male reproductive cells and rabbit tissues were examined. In addition, the nutritional and chemical composition of both bee products was investigated. Fresh and freeze-dried samples were used to isolate probiotic bacteria identified by MALDI-TOF MS Biotyper. Lactobacilli, especially <em>Apilactobacillus kunkeii, Lactiplantibacillus pentosus, Lactiplantibacillus plantarum,</em> and <em>Secundilactobacillus malefermentas</em>, received the highest identification scores. The best results were observed when targeting <em>S. aureus, S. epidermidis</em>, and <em>S. vitulinus</em>. The freeze-dried materials were tested on rabbit spermatozoa and testicular fragments to ascertain the effects of DM and DL. The results showed that the samples significantly reduced the amount of reactive oxygen species in the reproductive structures which resulted in a decrease in lipid peroxidation. Furthermore, testicular fragments treated <em>in vitro</em> with DM and DL produced considerably more testosterone and cholesterol; crude protein, nitrogenous compounds and fat were all present in higher amounts in DL while the content of reducing sugars was higher in DM.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"149 ","pages":"Pages 144-157"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143139496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deep-eutectic solvent extraction of ginsenosides and ginseng polysaccharides from ginseng residue and utilization of post-extraction residue 人参渣中人参皂苷和人参多糖的深度共晶溶剂提取及提取后渣的利用

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-02-01 DOI: 10.1016/j.procbio.2024.12.003

Zhaofeng Guan , Lina Zhou , Hao Wu , Zhenyu Chu , Mingjun Liu , Jun Zhou

{"title":"Deep-eutectic solvent extraction of ginsenosides and ginseng polysaccharides from ginseng residue and utilization of post-extraction residue","authors":"Zhaofeng Guan , Lina Zhou , Hao Wu , Zhenyu Chu , Mingjun Liu , Jun Zhou","doi":"10.1016/j.procbio.2024.12.003","DOIUrl":"10.1016/j.procbio.2024.12.003","url":null,"abstract":"<div><div>In this study, ginsenosides and polysaccharides were extracted from ginseng residue (GR) using a green method combining deep eutectic solvent (DES) and ultrasound. The results showed that the optimal extraction conditions of ginsenosides optimized by orthogonal test were choline chloride/propionic acid ratio of 1:3, moisture content of 30 %, solid-liquid ratio of 1:25, and ultrasound time of 30 min, which resulted in the yield of ginsenosides of 36.76 ± 1.46 mg/g, and improved the efficiency by 218.27 % compared with that of the traditional 70 % ethanol extraction method. The ginseng polysaccharides obtained by further purification of the extract showed different in vitro antioxidant activities, with scavenging rates of 91.2 %, 98.3 %, and 96.4 % for hydroxyl radicals, DPPH, and superoxide radicals, respectively, and Fe(Ⅱ) chelating activity at 93.5 %. The extracted GR showed good adsorption properties for glucose, sodium nitrite, cholesterol, and sodium cholate by in vitro adsorption tests with adsorption rates of 60 %, 93.3 %, 87.05 %, and 72.95 %, respectively. In conclusion, the use of DES as an extraction solvent has certain application prospects for GR reuse.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"149 ","pages":"Pages 99-110"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143139499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chitosan functionalized with EDTA as a new support for enzyme immobilization and its application on enzymatic resolution of (R,S)-1-phenylethanol EDTA功能化壳聚糖作为固定化酶的新载体及其在酶解（R，S）-1-苯乙醇中的应用

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-02-01 DOI: 10.1016/j.procbio.2024.12.004

Renata R. Magalhães , Paloma G. Abrantes , Poliana G. Abrantes , Israel F. Costa , Ercules E.S. Teotonio , Juliana A. Vale

{"title":"Chitosan functionalized with EDTA as a new support for enzyme immobilization and its application on enzymatic resolution of (R,S)-1-phenylethanol","authors":"Renata R. Magalhães , Paloma G. Abrantes , Poliana G. Abrantes , Israel F. Costa , Ercules E.S. Teotonio , Juliana A. Vale","doi":"10.1016/j.procbio.2024.12.004","DOIUrl":"10.1016/j.procbio.2024.12.004","url":null,"abstract":"<div><div>This research demonstrates the immobilization of lipases from <em>Burkholderia cepacia</em> (BCL), <em>Candida rugosa</em> (CRL)<em>,</em> and <em>Aspergillus niger</em> (ANL) on chitosan functionalized with EDTA (CHT-EDTA) as support, and its employment as a biocatalyst. The adsorption capacity of lipase onto the CHT-EDTA surface was observed to increase with contact time and an optimal pH value (pH 6,5). Enzymatic activity and stability of lipases before and after conjugation to the support were evaluated in different conditions (pH, temperature, and organic solvents) and then compared with those of the free one. Conformational changes were investigated using Fourier Transform Infrared spectroscopy (FTIR), Scanning Electron Microscopy (SEM), and thermogravimetric analysis (TGA). The results revealed that 112 mg/g for BCL/CHT-EDTA was the most significant amount of adsorbed lipase. The immobilized enzymes demonstrated enzymatic activity of 1.539 U/g for BCL/CHT-EDTA, followed by CRL/ and ANL/CHT-EDTA with 1.409 and 1.063 U/g, respectively. BCL/CHT-EDTA was then chosen to study the resolution of <em>R,S</em>-1-phenylethanol, achieving 99.9 % of <span><math><msub><mrow><mi>ee</mi></mrow><mrow><mi>P</mi></mrow></msub></math></span>, 82 % of <span><math><msub><mrow><mi>ee</mi></mrow><mrow><mi>S</mi></mrow></msub></math></span>, and 45 % of conversion (E > 200).</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"149 ","pages":"Pages 128-136"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143139491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing thermostability of lysine hydroxylase via a semi-rational design 半合理设计提高赖氨酸羟化酶的热稳定性

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-02-01 DOI: 10.1016/j.procbio.2024.12.005

Chengjuan Hu , Zhijie Zheng , Yue Zhang , Feifei Chen , Alei Zhang , Kequan Chen , Peicheng Luo

{"title":"Enhancing thermostability of lysine hydroxylase via a semi-rational design","authors":"Chengjuan Hu , Zhijie Zheng , Yue Zhang , Feifei Chen , Alei Zhang , Kequan Chen , Peicheng Luo","doi":"10.1016/j.procbio.2024.12.005","DOIUrl":"10.1016/j.procbio.2024.12.005","url":null,"abstract":"<div><div>(2<em>S</em>,4 <em>R</em>)-4-Hydroxylysine (4-OH-Lys), a derivative of L-lysine, possesses a unique chemical structure that makes it a crucial precursor for the synthesis of pharmaceutical molecules, with extensive applications in the pharmaceutical and biochemical industries. Lysine hydroxylase (K4H) catalyzes the conversion of L-lysine to 4-OH-Lys, offering advantages such as mild reaction conditions, straightforward reaction steps, good regioselectivity, and high catalytic efficiency compared to chemical synthesis and natural extraction methods. However, the low thermostability of K4H hinders its application in large-scale production. In this study, we employed a semi-rational design approach, guided by ΔΔG folding free energy calculations and message-passing neural networks to enhance the thermostability of K4H. After two rounds of evolution, we identified two beneficial mutants: M25 (S101P/Q257M) and M32 (Q257M/V298I). Thermostability assessments revealed that the half-lives (t<sub>1/2</sub>) of M25 and M32 at 40 °C were 23.9-fold and 13.3-fold higher than that of the wild-type (WT), with melting temperatures (T<sub>m</sub>) exceeding those of WT by 4.2 °C and 8.3 °C, respectively. Molecular dynamics simulations illuminated the mechanisms underlying this enhanced thermostability. This work provides valuable insights into the thermostability of K4H and yields key mutants that are promising candidates for practical production of 4-OH-Lys.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"149 ","pages":"Pages 111-120"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143139249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellulase immobilization within zeolitic imidazolate frameworks by in situ encapsulation 原位包封法固定化咪唑沸石框架内的纤维素酶

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-02-01 DOI: 10.1016/j.procbio.2024.12.001

Shanshan Tong , Chaozhong Xu , Kanghong Wang, Jia Ouyang, Xiaoli Gu

{"title":"Cellulase immobilization within zeolitic imidazolate frameworks by in situ encapsulation","authors":"Shanshan Tong , Chaozhong Xu , Kanghong Wang, Jia Ouyang, Xiaoli Gu","doi":"10.1016/j.procbio.2024.12.001","DOIUrl":"10.1016/j.procbio.2024.12.001","url":null,"abstract":"<div><div>The industrial applications of cellulase are often restricted by its high cost and poor stability under extreme conditions. In this study, cellulase was immobilized using zeolitic imidazolate framework-8 (ZIF-8) through a one-pot encapsulation method, resulting in the formation of cellulase@ZIF-8 composite designed to address these challenges. The effects of varying Zn²⁺/2-methylimidazole molar ratios and different amounts of cellulase on the properties of cellulase@ZIF-8 were systematically investigated. Cellulase was found to act as a nucleation site, accelerating the formation of cellulase@ZIF-8 while promoting controlled crystal growth. At lower Zn²⁺/2-methylimidazole ratios, cross-shaped cellulase@ZIF-8 crystals with moderate enzymatic performance were obtained. Conversely, at higher Zn²⁺/2-methylimidazole ratios, the resulting spindle-shaped cellulase@ZIF-8 crystals exhibited superior enzyme activity of 327.8 U/g, and a relative activity of 88.6 %. Furthermore, this composite demonstrates excellent thermal and storage stability. The immobilized enzyme retained 92.8 % of its activity at a temperature of 70 °C. Additionally, it maintained 69.8 % of its relative enzymatic activity after undergoing five cycles. These findings have significant implications for the future application of cellulase@ZIF-8 composites in efficient and cost-effective lignocellulosic bioconversion.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"149 ","pages":"Pages 65-73"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143139251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cofactor immobilization for efficient dehydrogenase driven upgrading of xylose 辅助因子固定化高效脱氢酶驱动木糖升级

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-02-01 DOI: 10.1016/j.procbio.2024.11.028

Karolina Bachosz , Agnieszka Rybarczyk , Adam Piasecki , Jakub Zdarta , Anne S. Meyer , Teofil Jesionowski

{"title":"Cofactor immobilization for efficient dehydrogenase driven upgrading of xylose","authors":"Karolina Bachosz , Agnieszka Rybarczyk , Adam Piasecki , Jakub Zdarta , Anne S. Meyer , Teofil Jesionowski","doi":"10.1016/j.procbio.2024.11.028","DOIUrl":"10.1016/j.procbio.2024.11.028","url":null,"abstract":"<div><div>In this study, the coupled immobilization on nanosilica of xylose dehydrogenase and alcohol dehydrogenase was accomplished with high efficiency, which was 90 %. Moreover, immobilization of the cofactors oxidized and reduced nicotinamide adenine dinucleotide, i.e., NAD<sup>+</sup> and NADH, on silica material was examined and the impact on the effectiveness of the process was determined. The highest efficiency of NAD<sup>+</sup> immobilization was found to be 56 %, which was obtained after 24 h of immobilization at 30 °C, pH 7 For NADH, the best immobilization efficiency was 53 % which was achieved after 24 h at 25 °C, pH 7. The <em>K</em><sub>M</sub> and <em>V</em><sub>max</sub> values were determined for various configurations of the biocatalytic systems showing, as expected, that immobilization of the enzymes decreased the catalytic rate (<em>V</em><sub>max</sub>) and slightly increased the <em>K</em><sub>M</sub>, but verifying that the immobilization of the cofactors did not significantly affect the kinetics, but would enable high conversion, and potentially continued enzymatic reaction. The use of the system configuration with co-immobilized enzymes, immobilized NAD<sup>+</sup> and immobilized NADH thus allowed for obtaining over 90 % efficiency of xylose conversion in one batch, which was significantly higher than the systems with single free or only one immobilized cofactor. Using UV-Vis measurements, it was confirmed that effective cofactor regeneration occurred in the systems with immobilized components thus allowing for sustained enzyme catalyzed upgrading of xylose to xylonic acid.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"149 ","pages":"Pages 36-44"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143139876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A renewable natural resource for ferulic acid; An efficient precursor in biotechnological production of vanillin and strategies to enhance the yield of bio-vanillin from ferulic acid - Review 阿魏酸的可再生天然资源；生物技术生产香兰素的有效前体及提高阿魏酸生物香兰素收率的策略综述

IF 3.7 3区生物学

Process Biochemistry Pub Date : 2025-02-01 DOI: 10.1016/j.procbio.2024.12.012

Dhesiga Krishnan, Manian Rameshpathy

引用次数: 0