Progress in Biomaterials最新文献_第7页

Promotion of nerve regeneration by biodegradable nanofibrous scaffold following sciatic nerve transection in rats. 生物可降解纳米纤维支架促进大鼠坐骨神经横断后神经再生。

IF 4.9 3区医学

Progress in Biomaterials Pub Date : 2021-03-01 Epub Date: 2021-03-08 DOI: 10.1007/s40204-021-00151-w

Farshad Moharrami Kasmaie, Fatemeh Zamani, Sara Sayad-Fathi, Arash Zaminy

{"title":"Promotion of nerve regeneration by biodegradable nanofibrous scaffold following sciatic nerve transection in rats.","authors":"Farshad Moharrami Kasmaie, Fatemeh Zamani, Sara Sayad-Fathi, Arash Zaminy","doi":"10.1007/s40204-021-00151-w","DOIUrl":"https://doi.org/10.1007/s40204-021-00151-w","url":null,"abstract":"Peripheral nerve injuries (PNIs) are one of the common causes of morbidity and disability worldwide. Autograft is considered the gold standard treatment for PNIs. However, due to the complications associated with autografts, other sources are considered as alternatives. Recently, electrospun nanofibrous scaffolds have received wide attention in the field of tissue engineering. Exogenous tubular constructs with uniaxially aligned topographical cues to enhance the axonal re-growth are needed to bridge large nerve gaps between proximal and distal ends. Although several studies have used PLGA/PCL, but few studies have been conducted on developing a two-layer scaffold with aligned fibers properly orientated along the axis direction of the sciatic nerve to meet the physical properties required for suturing, transplantation, and nerve regeneration. In this study, we sought to design and develop PLGA-PCL-aligned nanofibers. Following the conventional examinations, we implanted the scaffolds into 7-mm sciatic nerve gaps in a rat model of nerve injury. Our in vivo evaluations did not show any adverse effects, and after eight weeks, an acceptable improvement was noted in the electrophysiological, functional, and histological analyses. Thus, it can be concluded that nanofiber scaffolds can be used as a reliable approach for repairing PNIs. However, further research is warranted.","PeriodicalId":20691,"journal":{"name":"Progress in Biomaterials","volume":"10 1","pages":"53-64"},"PeriodicalIF":4.9,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s40204-021-00151-w","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25447695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Injectable hyaluronic acid-based antibacterial hydrogel adorned with biogenically synthesized AgNPs-decorated multi-walled carbon nanotubes. 以生物合成的agnps装饰的多壁碳纳米管装饰的可注射透明质酸抗菌水凝胶。

IF 4.9 3区医学

Progress in Biomaterials Pub Date : 2021-03-01 Epub Date: 2021-03-26 DOI: 10.1007/s40204-021-00155-6

Pooyan Makvandi, Milad Ashrafizadeh, Matineh Ghomi, Masoud Najafi, Hamid Heydari Sheikh Hossein, Ali Zarrabi, Virgilio Mattoli, Rajender S Varma

{"title":"Injectable hyaluronic acid-based antibacterial hydrogel adorned with biogenically synthesized AgNPs-decorated multi-walled carbon nanotubes.","authors":"Pooyan Makvandi, Milad Ashrafizadeh, Matineh Ghomi, Masoud Najafi, Hamid Heydari Sheikh Hossein, Ali Zarrabi, Virgilio Mattoli, Rajender S Varma","doi":"10.1007/s40204-021-00155-6","DOIUrl":"https://doi.org/10.1007/s40204-021-00155-6","url":null,"abstract":"Injectable materials have shown great potential in tissue engineering applications. However, bacterial infection is one of the main challenges in using these materials in the field of regenerative medicine. In this study, biogenically synthesized silver nanoparticle-decorated multi-walled carbon nanotubes (Ag/MWCNTs) were deployed for adorning biogenic-derived AgNPs which were subsequently used in the preparation of thermosensitive hydrogels based on hyaluronic acid encompassing these green-synthesized NPs. The antibacterial capacity of AgNPs decorated on MWCNTs synthesized through Camellia sinensis extract in an organic solvent-free medium displayed a superior activity by inhibiting the growth of Gram-negative (E. coli and Klebsiella) and Gram-positive (S. aureus and E. faecalis). The injectable hydrogel nanocomposites demonstrated good mechanical properties, as well. The thermosensitive hyaluronic acid-based hydrogels also exhibited Tgel below the body temperature, indicating the transition from liquid-like behavior to elastic gel-like behavior. Such a promising injectable nanocomposite could be applied as liquid, pomade, or ointment to enter wound cavities or bone defects and subsequently its transition in situ to gel form at human body temperature bodes well for their immense potential application in the biomedical sector.","PeriodicalId":20691,"journal":{"name":"Progress in Biomaterials","volume":"10 1","pages":"77-89"},"PeriodicalIF":4.9,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s40204-021-00155-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25517756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Tissue engineering of collagen scaffolds crosslinked with plant based polysaccharides. 用植物多糖交联胶原支架的组织工程学。

IF 4.9 3区医学

Progress in Biomaterials Pub Date : 2021-03-01 Epub Date: 2021-02-18 DOI: 10.1007/s40204-021-00149-4

Rohit Rekulapally, K Udayachandrika, Sirisha Hamlipur, Anuja Sasidharan Nair, Biswajit Pal, Shashi Singh

{"title":"Tissue engineering of collagen scaffolds crosslinked with plant based polysaccharides.","authors":"Rohit Rekulapally, K Udayachandrika, Sirisha Hamlipur, Anuja Sasidharan Nair, Biswajit Pal, Shashi Singh","doi":"10.1007/s40204-021-00149-4","DOIUrl":"10.1007/s40204-021-00149-4","url":null,"abstract":"Ideally, a bioscaffold should mimic the characteristics of an extracellular matrix of a living organ of interest. The present study deals with the formation of composite scaffolds of collagen with gum arabic. Collagen was cross-linked with oxidized gum arabic having aldehyde groups to form a porous block. By changing the oxidation level of gum arabic, incorporation of the polysaccharides into the scaffold could be varied resulting in scaffolds with variable polysaccharide to protein content. A series of scaffolds were made by altering collagen concentration and oxidation level of gum arabic. The scaffolds were tested for their physical properties, stability, biocompatibility and ability to support the cell growth. Results implied that variable polysaccharide incorporation into the scaffolds was possible depending on the oxidation level of gum arabic which could influence the swelling behavior. The scaffolds showed non-toxic behavior towards the mesenchymal stem cells and nucleus pulposa cells using viability assay in culture conditions up to 30 days; the growth of cells was seen at all combinations of gels. Nucleus pulposa cells were able to maintain their phenotype in the GACO gels. The studies show that these scaffolds are potential candidates in applications, such as tissue engineering, and can be designed to match the requirement of different cell/tissues as per their ECM.","PeriodicalId":20691,"journal":{"name":"Progress in Biomaterials","volume":"10 1","pages":"29-41"},"PeriodicalIF":4.9,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8021656/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25388218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrospun PCL scaffold modified with chitosan nanoparticles for enhanced bone regeneration. 壳聚糖纳米颗粒修饰电纺丝PCL支架增强骨再生。

IF 4.9 3区医学

Progress in Biomaterials Pub Date : 2021-03-01 Epub Date: 2021-03-13 DOI: 10.1007/s40204-021-00153-8

Ameneh Seddighian, Fariba Ganji, Mohamadreza Baghaban-Eslaminejad, Fatemeh Bagheri

{"title":"Electrospun PCL scaffold modified with chitosan nanoparticles for enhanced bone regeneration.","authors":"Ameneh Seddighian, Fariba Ganji, Mohamadreza Baghaban-Eslaminejad, Fatemeh Bagheri","doi":"10.1007/s40204-021-00153-8","DOIUrl":"https://doi.org/10.1007/s40204-021-00153-8","url":null,"abstract":"The encapsulation of ascorbic acid within chitosan nanoparticles (CHNs), embedded in a fibrous structure of a dexamethasone (Dex)-loaded PCL scaffold, provides a new plan for osteogenic differentiation of mesenchymal stem cells. This electrospun PCL fibrous scaffold can release Dex, as bone differentiation initiator, and ascorbic acid, as bone differentiation enhancer, in an approximately sustained release pattern for about 2 weeks. Ascorbic acid-loaded CHNs were prepared by electrospraying a mixture of chitosan and ascorbic acid, and Dex-containing PCL fibers were prepared by electrospinning a mixture of PCL and Dex. The final PCL/chitosan bilayer scaffolds were obtained by the sequential employment of electrospinning and electrospraying methods. Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR) confirmed that the CHNs were successfully incorporated into the fibrous PCL matrix. The improved proliferation of hMSCs cultured on the PCL/chitosan scaffolds was also verified. Osteogenic assays showed an increase in alkaline phosphatase activity and mineral deposits. The expression of bone-specific genes also confirmed the osteogenic differentiation of cells cultured on these PCL/chitosan bilayer scaffolds. Dual-drug-loaded PCL/chitosan scaffold enhanced the osteoblast differentiation of hMSC cells and can be served as a potential scaffold for bone tissue engineering.","PeriodicalId":20691,"journal":{"name":"Progress in Biomaterials","volume":"10 1","pages":"65-76"},"PeriodicalIF":4.9,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s40204-021-00153-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25471227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Engineered PLGA-PVP/VA based formulations to produce electro-drawn fast biodegradable microneedles for labile biomolecule delivery. 工程PLGA-PVP/VA为基础的配方，生产电拔快速可生物降解的微针，用于不稳定的生物分子递送。

IF 4.9 3区医学

Progress in Biomaterials Pub Date : 2020-12-01 Epub Date: 2020-11-03 DOI: 10.1007/s40204-020-00143-2

Valentina Onesto, Concetta Di Natale, Martina Profeta, Paolo Antonio Netti, Raffaele Vecchione

{"title":"Engineered PLGA-PVP/VA based formulations to produce electro-drawn fast biodegradable microneedles for labile biomolecule delivery.","authors":"Valentina Onesto, Concetta Di Natale, Martina Profeta, Paolo Antonio Netti, Raffaele Vecchione","doi":"10.1007/s40204-020-00143-2","DOIUrl":"https://doi.org/10.1007/s40204-020-00143-2","url":null,"abstract":"Biodegradable polymer microneedles (MNs) are recognized as non-toxic, safe and stable systems for advanced drug delivery and cutaneous treatments, allowing a direct intradermal delivery and in some cases a controlled release. Most of the microneedles found in the literature are fabricated by micromolding, which is a multistep thus typically costly process. Due to industrial needs, mold-free methods represent a very intriguing approach in microneedle fabrication. Electro-drawing (ED) has been recently proposed as an alternative fast, mild temperature and one-step strategy to the mold-based techniques for the fabrication of poly(lactic-co-glycolic acid) (PLGA) biodegradable MNs. In this work, taking advantage of the flexibility of the ED technology, we engineered microneedle inner microstructure by acting on the water-in-oil (W/O) precursor emulsion formulation to tune drug release profile. Particularly, to promote a faster release of the active pharmaceutical ingredient, we substituted part of PLGA with poly(1-vinylpyrrolidone-co-vinyl acetate) (PVP/VA), as compared to the PLGA alone in the matrix material. Moreover, we introduced lecithin and maltose as emulsion stabilizers. Microneedle inner structural analysis as well as collagenase entrapment efficiency, release and activity of different emulsion formulations were compared to reach an interconnected porosity MN structure, aimed at providing an efficient protein release profile. Furthermore, MN mechanical properties were examined as well as its ability to pierce the stratum corneum on a pig skin model, while the drug diffusion from the MN body was monitored in an in vitro collagen-based dermal model at selected time points.","PeriodicalId":20691,"journal":{"name":"Progress in Biomaterials","volume":"9 4","pages":"203-217"},"PeriodicalIF":4.9,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s40204-020-00143-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38561873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

Recent advances in the formulation of PLGA microparticles for controlled drug delivery. 用于控制药物输送的聚乳酸丙烯酸酯微粒配方的最新进展。

IF 4.9 3区医学

Progress in Biomaterials Pub Date : 2020-12-01 Epub Date: 2020-10-15 DOI: 10.1007/s40204-020-00139-y

Elena Lagreca, Valentina Onesto, Concetta Di Natale, Sara La Manna, Paolo Antonio Netti, Raffaele Vecchione

{"title":"Recent advances in the formulation of PLGA microparticles for controlled drug delivery.","authors":"Elena Lagreca, Valentina Onesto, Concetta Di Natale, Sara La Manna, Paolo Antonio Netti, Raffaele Vecchione","doi":"10.1007/s40204-020-00139-y","DOIUrl":"10.1007/s40204-020-00139-y","url":null,"abstract":"Polymeric microparticles (MPs) are recognized as very popular carriers to increase the bioavailability and bio-distribution of both lipophilic and hydrophilic drugs. Among different kinds of polymers, poly-(lactic-co-glycolic acid) (PLGA) is one of the most accepted materials for this purpose, because of its biodegradability (due to the presence of ester linkages that are degraded by hydrolysis in aqueous environments) and safety (PLGA is a Food and Drug Administration (FDA)-approved compound). Moreover, its biodegradability depends on the number of glycolide units present in the structure, indeed, lower glycol content results in an increased degradation time and conversely a higher monomer unit number results in a decreased time. Due to this feature, it is possible to design and fabricate MPs with a programmable and time-controlled drug release. Many approaches and procedures can be used to prepare MPs. The chosen fabrication methodology influences size, stability, entrapment efficiency, and MPs release kinetics. For example, lipophilic drugs as chemotherapeutic agents (doxorubicin), anti-inflammatory non-steroidal (indomethacin), and nutraceuticals (curcumin) were successfully encapsulated in MPs prepared by single emulsion technique, while water-soluble compounds, such as aptamer, peptides and proteins, involved the use of double emulsion systems to provide a hydrophilic compartment and prevent molecular degradation. The purpose of this review is to provide an overview about the preparation and characterization of drug-loaded PLGA MPs obtained by single, double emulsion and microfluidic techniques, and their current applications in the pharmaceutical industry.Graphic abstract.","PeriodicalId":20691,"journal":{"name":"Progress in Biomaterials","volume":"9 4","pages":"153-174"},"PeriodicalIF":4.9,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7718366/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38586177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design, characterization and in vitro evaluation of thin films enriched by tannic acid complexed by Fe(III) ions. 单宁酸与铁(III)离子络合富膜的设计、表征及体外评价。

IF 4.9 3区医学

Progress in Biomaterials Pub Date : 2020-12-01 Epub Date: 2020-11-21 DOI: 10.1007/s40204-020-00146-z

B Kaczmarek, O Mazur, O Miłek, M Michalska-Sionkowska, A Das, A Jaiswal, J Vishnu, K Tiwari, A Sionkowska, A M Osyczka, G Manivasagam

{"title":"Design, characterization and in vitro evaluation of thin films enriched by tannic acid complexed by Fe(III) ions.","authors":"B Kaczmarek, O Mazur, O Miłek, M Michalska-Sionkowska, A Das, A Jaiswal, J Vishnu, K Tiwari, A Sionkowska, A M Osyczka, G Manivasagam","doi":"10.1007/s40204-020-00146-z","DOIUrl":"https://doi.org/10.1007/s40204-020-00146-z","url":null,"abstract":"Materials based on carbohydrate polymers may be used for biomedical application. However, materials based on natural polymers have weak physicochemical properties. Thereby, there is a challenge to improve their properties without initiation of toxicity. The alternative method compared to toxic chemical agents' addition is the use of metal complexation method. In this study, chitosan/tannic acid mixtures modified by Fe(III) complexation are proposed and tested for potential applications as wound dressings. Thereby, surface properties, blood compatibility as well as platelet adhesion was tested. In addition, the periodontal ligament stromal cells compatibility studies were carried out. The results showed that the iron(III) addition to chitosan/tannic acid mixture improves properties due to a decrease in the surface free energy and exhibited a reduction in the hemolysis rate (below 5%). Moreover, cells cultured on the surface of films with Fe(III) showed higher metabolic activity. The current findings allow for the medical application of the proposed materials as wound dressings.","PeriodicalId":20691,"journal":{"name":"Progress in Biomaterials","volume":"9 4","pages":"249-257"},"PeriodicalIF":4.9,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s40204-020-00146-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38630466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Surface modification of electrospun silk/AMOX/PVA nanofibers by dielectric barrier discharge plasma: physiochemical properties, drug delivery and in-vitro biocompatibility. 电介质阻挡放电等离子体对电纺丝/AMOX/PVA纳米纤维的表面改性:理化性能、给药性能和体外生物相容性

IF 4.9 3区医学

Progress in Biomaterials Pub Date : 2020-12-01 Epub Date: 2020-11-18 DOI: 10.1007/s40204-020-00144-1

Namita Ojah, Rajiv Borah, Gazi Ameen Ahmed, Manabendra Mandal, Arup Jyoti Choudhury

{"title":"Surface modification of electrospun silk/AMOX/PVA nanofibers by dielectric barrier discharge plasma: physiochemical properties, drug delivery and in-vitro biocompatibility.","authors":"Namita Ojah, Rajiv Borah, Gazi Ameen Ahmed, Manabendra Mandal, Arup Jyoti Choudhury","doi":"10.1007/s40204-020-00144-1","DOIUrl":"10.1007/s40204-020-00144-1","url":null,"abstract":"The naturally obtained protein Bombyxmori silk is a biocompatible polymer with excellent mechanical properties and have the potential in controlled drug delivery applications. In this work, we have demonstrated dielectric barrier discharge (DBD) oxygen (O2) plasma surface modified electrospun Bombyxmori silk/Amoxicillin hydrochloride trihydrate (AMOX)/polyvinyl alcohol (PVA) nanofibers for drug release applications with controlled plasma treatment duration (1-10 min). The findings indicate that plasma treated electrospun nanofibers for 1-3 min exhibited significant enhancement in tensile strength, Young's modulus, wettability and surface energy. The plasma treated electrospun nanofibers for 1-5 min showed remarkable increase in AMOX released rate, whereas the electrospun nanofibers treated with plasma irradiation beyond 5 min showed only marginal increase. Moreover, the plasma treated nanofibers also exhibited good antibacterial activity against both E. coli (gram negative) and S. aureus (gram positive) bacteria. The untreated and the plasma treated silk/AMOX/PVA electrospun nanofibers for 1-3 min showed enhanced viability of primary adipose derived mesenchymal stem cells (ADMSCs) growth on them and much less hemolysis activity (< 5%). The in vitro biocompatibility of various electrospun nanofibers were further corroborated by live/dead imaging and cytoskeletal architecture assessment demonstrating enhanced cell adhesion and spreading on the plasma treated nanofibers for 1-3 min. The findings of the present study suggest that the silk/AMOX/PVA electrospun nanofibers with plasma treatment (1-3 min) due to their enhanced drug release ability and biocompatibility can be used as potential wound dressing applications.","PeriodicalId":20691,"journal":{"name":"Progress in Biomaterials","volume":"9 4","pages":"219-237"},"PeriodicalIF":4.9,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s40204-020-00144-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38618854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

A cost effective SiO₂-CaO-Na₂O bio-glass derived from bio-waste resources for biomedical applications. 从生物废物资源中提取的具有成本效益的用于生物医学应用的SiO2-CaO-Na2O生物玻璃。

IF 4.9 3区医学

Progress in Biomaterials Pub Date : 2020-12-01 Epub Date: 2020-11-19 DOI: 10.1007/s40204-020-00145-0

Srinath Palakurthy, K Venugopal Reddy, Sushil Patel, P Abdul Azeem

{"title":"A cost effective SiO2-CaO-Na2O bio-glass derived from bio-waste resources for biomedical applications.","authors":"Srinath Palakurthy, K Venugopal Reddy, Sushil Patel, P Abdul Azeem","doi":"10.1007/s40204-020-00145-0","DOIUrl":"https://doi.org/10.1007/s40204-020-00145-0","url":null,"abstract":"The present paper describes the in vitro bioactivity, cytocompatibility and degradation performance of SiO2-CaO-Na2O bio-glass synthesized using bio-waste. Egg shells and rice husk ash (RHA) bio-wastes were used as sources of calcium oxide (CaO) and silica (SiO2), respectively. Glass samples were obtained by melt-quenching technique. Bioactivity was studied using in vitro experiments in simulated body fluid (SBF), degradation behaviour was evaluated in Tris-HCl buffer solutions recommended by ISO 10993-14 standards and cytocompatibility was estimated using MTT assay. The formation of hydroxyapatite was characterized by XRD, FTIR and SEM-EDS after soaking the glass samples in SBF solution. XRD confirmed the phase of hydroxyapatite with its standard JCPDS data. FTIR analyses revealed the occurrence of distinctive functional groups related to hydroxyapatite. Surface micrographs showed the agglomerated globular shape morphology of hydroxyapatite, while EDS analysis confirmed the existence of biological elements of apatite such as Ca, P and O. Degradation study results showed that the glass thus prepared has considerable controlled degradation rate. MTT assay revealed the cytocompatibility nature for different dosages (1000-50 μg/mL) of the prepared glass with MG-63 cells. These results perfectly established that egg shells and RHA are potentially beneficial resources for the production of bio-glasses.","PeriodicalId":20691,"journal":{"name":"Progress in Biomaterials","volume":"9 4","pages":"239-248"},"PeriodicalIF":4.9,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s40204-020-00145-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38623419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Enhanced brain targeting efficiency using 5-FU (fluorouracil) lipid-drug conjugated nanoparticles in brain cancer therapy. 在脑癌治疗中使用 5-FU（氟尿嘧啶）脂质药物共轭纳米粒子提高脑靶向效率。

IF 4.9 3区医学

Progress in Biomaterials Pub Date : 2020-12-01 Epub Date: 2020-11-30 DOI: 10.1007/s40204-020-00147-y

Gajanan Shinde, Sangita Shiyani, Santosh Shelke, Rashmi Chouthe, Deepak Kulkarni, Khushboo Marvaniya

{"title":"Enhanced brain targeting efficiency using 5-FU (fluorouracil) lipid-drug conjugated nanoparticles in brain cancer therapy.","authors":"Gajanan Shinde, Sangita Shiyani, Santosh Shelke, Rashmi Chouthe, Deepak Kulkarni, Khushboo Marvaniya","doi":"10.1007/s40204-020-00147-y","DOIUrl":"10.1007/s40204-020-00147-y","url":null,"abstract":"The present investigation was aimed to synthesize, optimize, and characterize lipid/drug conjugate nanoparticles for delivering 5-fluorouracil (5-FU) to treat brain cancer. The Box-Behnken design was used to optimize the formulation, evaluate the particle size, entrapment efficiency, morphology, in vitro drug release study, and stability profiles. The in vitro performance was executed using cell line studies. The in vivo performance was carried out for pharmacokinetic studies, sterility test, biodistribution studies, and distribution lipid-drug conjugated (LDC) nanoparticles in the brain. Particle size, zeta potential, entrapment efficiency, and morphology of the optimized formulation demonstrated desirable results. In vitro release pattern showed initial fast release, followed by sustained release up to 48 h. Cytotoxic effects of blank stearic acid nanoparticles, LDC nanoparticles, and 5-FU solution on human glioma cell lines U373 MG cell showed more cytotoxicity by LDC-NPs compared to others. The values reported for LDC (AUC = 19.37 ± 0.09 µg/mL h and VD 2.4 ± 0.24 mL) and pure drug (AUC = 8.37 ± 0.04 µg/mL h and VD = 5.24 ± 0.29 mL) indicate higher concentrations of LDC in systemic circulation, while pure 5-FU was found to be largely available in tissue rather than blood circulation. The t1/2 for LDC represents an approximate rise by ninefold, while MRT (12.10 ± 0.44 h) denotes 12-fold rise than pure 5-FU indicating the prolonged circulation of LDC. Free 5-FU concentration in the brain was maximum (5.24 ± 0.01 μg/g) after 3 h, while for the optimized formulation of LDC it was twofold greater estimated as 11.52 ± 0.32 μg/g. In conclusion, the efficiency of 5-FU to treat the brain is increased when it is formulated with LDC nanoparticles.","PeriodicalId":20691,"journal":{"name":"Progress in Biomaterials","volume":"9 4","pages":"259-275"},"PeriodicalIF":4.9,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7718375/pdf/40204_2020_Article_147.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38654826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0