Nanohydroxyapatite incorporated photocrosslinked gelatin methacryloyl/poly(ethylene glycol)diacrylate hydrogel for bone tissue engineering.

IF 4.4 3区医学 Q2 ENGINEERING, BIOMEDICAL

Progress in Biomaterials Pub Date : 2021-03-01 Epub Date: 2021-03-26 DOI:10.1007/s40204-021-00150-x

Sreekanth Sreekumaran, Anitha Radhakrishnan, Arun A Rauf, G Muraleedhara Kurup

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引用次数: 18

Abstract

The development of novel strategies that aim to augment the regenerative potential of bone is critical for devising better treatment options for bone defects or injuries. Facilitation of bone repair and regeneration utilizing composite hydrogels that simulates bone matrix is emerging as a viable approach in bone tissue engineering. The present study aimed to develop nanohydroxyapatite-incorporated gelatin methacryloyl (GelMA)/poly(ethylene glycol) diacrylate (PEGDA) hydrogel (GMPH hydrogel). A facile blending and photocrosslinking approach was employed to incorporate nanohydroxyapatite into the inter-crosslinked polymeric hydrogel network to obtain an ECM mimicking matrix for assisting bone tissue regeneration. Chemical characterization of GelMA and the GMPH hydrogel was carried out using FTIR and ¹H NMR. Physical properties of GMPH, such as gelation, swelling and degradation ratios, and internal morphology, signified the suitability of GMPH hydrogel for tissue engineering. Cell viability assay demonstrated a healthy proliferation of MG63 osteoblast cells in GMPH hydrogel extracted growth medium, indicating the hydrogel's cytocompatibility and suitability for bone tissue engineering. Our study documented the fabrication of a novel GelMA/PEGDA-nanohydroxyapatite hydrogel that possesses ideal physicochemical and biological properties for bone tissue engineering.

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纳米羟基磷灰石结合光交联明胶甲基丙烯/聚乙二醇二丙烯酸酯水凝胶用于骨组织工程。

旨在增强骨再生潜力的新策略的发展对于设计更好的骨缺损或骨损伤治疗方案至关重要。利用模拟骨基质的复合水凝胶促进骨修复和再生是骨组织工程中一种可行的方法。本研究旨在研制纳米羟基磷灰石-明胶甲基丙烯酰(GelMA)/聚乙二醇二丙烯酸酯(PEGDA)水凝胶(GMPH)。采用简单的混合和光交联方法将纳米羟基磷灰石纳入交联聚合物水凝胶网络中，以获得辅助骨组织再生的ECM模拟基质。利用FTIR和1H NMR对GelMA和GMPH水凝胶进行了化学表征。GMPH的物理性质，如凝胶、膨胀和降解率，以及内部形态，表明GMPH水凝胶适用于组织工程。细胞活力实验表明，MG63成骨细胞在GMPH水凝胶提取的生长培养基中具有良好的增殖能力，表明该水凝胶具有良好的细胞相容性和骨组织工程的适用性。我们的研究记录了一种新型GelMA/ pegda纳米羟基磷灰石水凝胶的制备，它具有理想的骨组织工程的物理化学和生物特性。

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来源期刊

Progress in Biomaterials MATERIALS SCIENCE, BIOMATERIALS-

CiteScore

9.60

自引率

4.10%

发文量

期刊介绍： Progress in Biomaterials is a multidisciplinary, English-language publication of original contributions and reviews concerning studies of the preparation, performance and evaluation of biomaterials; the chemical, physical, biological and mechanical behavior of materials both in vitro and in vivo in areas such as tissue engineering and regenerative medicine, drug delivery and implants where biomaterials play a significant role. Including all areas of: design; preparation; performance and evaluation of nano- and biomaterials in tissue engineering; drug delivery systems; regenerative medicine; implantable medical devices; interaction of cells/stem cells on biomaterials and related applications.