In-vitro cellular and in-vivo investigation of ascorbic acid and β-glycerophosphate loaded gelatin/sodium alginate injectable hydrogels for urinary incontinence treatment.

IF 4.4 3区 医学 Q2 ENGINEERING, BIOMEDICAL
Progress in Biomaterials Pub Date : 2021-06-01 Epub Date: 2021-06-24 DOI:10.1007/s40204-021-00160-9
Hessam Rezaei, Azadeh Asefnejad, Morteza Daliri-Joupari, Sedigheh Joughehdoust
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引用次数: 0

Abstract

Urinary incontinence is one of the most common disorders especially in adult women. In this study, cellular and in-vivo analyses were performed on (3-glycidyloxypropyl) trimethoxysilane (GPTMS) and CaCl2 cross-linked alginate and gelatin hydrogels containing β-glycerophosphate and ascorbic acid to evaluate the regenerative potential as injectable compression agents for the treatment of urinary incontinence. The hydrogels were prepared with different percentages of components and were named as GA1 (7.2% w/v gelatin, 6% w/v sodium alginate, 0.5:1w/w GPTMS, CaCl2 1% (wt) sodium alginate, 50 μg/mL ascorbic acid, 1.5 mg/mL β-glycerophosphate), GA2 (10% w/v gelatin, 8.5% w/v sodium alginate, 0.5:1 w/w GPTMS, CaCl2 1% (wt) sodium alginate, 50 μg/mL ascorbic acid, 1.5 mg/mL β-glycerophosphate), and GA3 (10% (w/v) gelatin, 8.5% w/v sodium alginate, 1:1 w/w GPTMS, CaCl2 1% (wt) sodium alginate, 50 μg/mL ascorbic acid, 1.5 mg/mL β-glycerophosphate) hydrogels. The results of cell studies showed that although all three samples supported cell adhesion and survival, the cellular behavior of the GA2 sample was better than the other samples. Animal tests were performed on the optimal GA2 sample, which showed that this hydrogel repaired the misfunction tissue in a rat model within 4 weeks and the molecular layer thickness was reached the normal tissue after this duration. It seems that these hydrogels, especially GA2 sample containing 10% (w/v) gelatin, 8.5% (w/v) sodium alginate, 0.5:1 (w/w) GPTMS, CaCl2 1% (wt) sodium alginate, 50 μg/mL ascorbic acid, and 1.5 mg/mL β-glycerophosphate, can act as an injetable hydrogel for urinary incontinence treatment without the need for repeating the injection.

抗坏血酸和β-甘油磷酸酯负载明胶/海藻酸钠注射水凝胶治疗尿失禁的体外细胞和体内研究。
尿失禁是最常见的疾病之一,尤其是在成年女性中。本研究对含有 β-甘油磷酸酯和抗坏血酸的(3-缩水甘油氧丙基)三甲氧基硅烷(GPTMS)和 CaCl2 交联海藻酸和明胶水凝胶进行了细胞和体内分析,以评估其作为治疗尿失禁的注射压缩剂的再生潜力。制备的水凝胶含有不同比例的成分,分别命名为 GA1(7.2% w/v 明胶、6% w/v 海藻酸钠、0.5:1w/w GPTMS、CaCl2 1%(重量)海藻酸钠、50 μg/mL 抗坏血酸、1.5 mg/mL β-甘油磷酸酯)、GA2(10% w/v 明胶、8.5% w/v 海藻酸钠、0.5:1 w/w GPTMS、CaCl2 1%(重量)海藻酸钠、50 μg/mL 抗坏血酸、1.5 mg/mL β-甘油磷酸酯)和 GA3(10%(重量)明胶、8.5%(重量)海藻酸钠、1:1 w/w GPTMS、CaCl2 1%(重量)海藻酸钠、50 μg/mL 抗坏血酸、1.5 mg/mL β-甘油磷酸酯)水凝胶。细胞研究结果表明,虽然所有三种样品都支持细胞粘附和存活,但 GA2 样品的细胞行为优于其他样品。对最佳 GA2 样品进行的动物试验表明,这种水凝胶在 4 周内修复了大鼠模型中功能失调的组织,并且在这段时间后,分子层厚度达到了正常组织的厚度。由此看来,这些水凝胶,尤其是含有 10%(重量比)明胶、8.5%(重量比)海藻酸钠、0.5:1(重量比)GPTMS、1%(重量比)海藻酸钠 CaCl2、50 μg/mL 抗坏血酸和 1.5 mg/mL β-甘油磷酸酯的 GA2 样品,可作为一种可注射的水凝胶用于治疗尿失禁,而无需重复注射。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Progress in Biomaterials
Progress in Biomaterials MATERIALS SCIENCE, BIOMATERIALS-
CiteScore
9.60
自引率
4.10%
发文量
35
期刊介绍: Progress in Biomaterials is a multidisciplinary, English-language publication of original contributions and reviews concerning studies of the preparation, performance and evaluation of biomaterials; the chemical, physical, biological and mechanical behavior of materials both in vitro and in vivo in areas such as tissue engineering and regenerative medicine, drug delivery and implants where biomaterials play a significant role. Including all areas of: design; preparation; performance and evaluation of nano- and biomaterials in tissue engineering; drug delivery systems; regenerative medicine; implantable medical devices; interaction of cells/stem cells on biomaterials and related applications.
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