Plant and Cell Physiology最新文献_第7页

The rice BRITTLE CULM 4 gene encodes a membrane protein involved in cellulose synthesis in the secondary cell wall. 水稻脆性culm4基因编码一种参与次级细胞壁纤维素合成的膜蛋白。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-08-21 DOI: 10.1093/pcp/pcaf096

Masatoshi Yamaguchi, Ami Sato, Daisuke Takahashi, Kazuhisa Mori, Ryota Fujimoto, Atsuko Miyagi, Eriko Sato, Toshiki Ishikawa, Ryosuke Sano, Tetsuya Kurata, Shiro Suzuki, Yasuko Kaneko, Maki Kawai-Yamada, Toshihisa Kotake

{"title":"The rice BRITTLE CULM 4 gene encodes a membrane protein involved in cellulose synthesis in the secondary cell wall.","authors":"Masatoshi Yamaguchi, Ami Sato, Daisuke Takahashi, Kazuhisa Mori, Ryota Fujimoto, Atsuko Miyagi, Eriko Sato, Toshiki Ishikawa, Ryosuke Sano, Tetsuya Kurata, Shiro Suzuki, Yasuko Kaneko, Maki Kawai-Yamada, Toshihisa Kotake","doi":"10.1093/pcp/pcaf096","DOIUrl":"https://doi.org/10.1093/pcp/pcaf096","url":null,"abstract":"The formation of secondary cell walls, which provide mechanical strength to the plant body, depends on numerous factors. Studies on rice brittle culm (bc) mutants allow us to identify these factors and gain insights into the mechanisms of secondary cell wall formation. Rice bc4 is a recessive bc mutant with fragile culms and leaves, similar to other bc mutants. We found that the bc4 mutant exhibited reduced cellulose content in the culm cell walls compared to the japonica cultivar Taichung 65 and the indica cultivar Kasalath, while hemicellulose content remained unchanged. Transmission electron microscopy revealed reduced cell wall thickness in the sclerenchyma cells of the bc4 culm, indicating that BC4 contributes to normal cellulose synthesis or deposition in secondary cell walls. Positional cloning and subsequent genome sequencing revealed that the BC4 gene encodes a four α-helical transmembrane protein with 205 amino acids, and that the bc4 mutation results in a premature termination codon in this gene. Four bc4 mutants generated from the japonica cultivar Nipponbare, using genome editing with the CRISPR/Cas9 system, exhibited reduced cellulose content along with bc phenotypes. Gene clustering analysis based on expression patterns and metabolomic analysis suggested that BC4 functions independently from secondary cell wall CesAs and COBRA-like protein. These results suggest that the BC4 protein is a newly identified factor involved in cellulose synthesis or deposition in the secondary cell walls of rice.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Cost of Survival: Mutation in a Barley Strigolactone Repressor HvD53A Impairs Photosynthesis but Increases Drought Tolerance. 生存代价：大麦独角麦内酯抑制因子HvD53A的突变损害了光合作用，但增加了耐旱性。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-08-12 DOI: 10.1093/pcp/pcaf095

Magdalena Korek, Weronika Buchcik, Beata Chmielewska, Agata Daszkowska-Golec, Irene M Fontana, Michael Melzer, Goetz Hensel, Jochen Kumlehn, Philip B Brewer, Glen R Uhrig, Marek Marzec

{"title":"The Cost of Survival: Mutation in a Barley Strigolactone Repressor HvD53A Impairs Photosynthesis but Increases Drought Tolerance.","authors":"Magdalena Korek, Weronika Buchcik, Beata Chmielewska, Agata Daszkowska-Golec, Irene M Fontana, Michael Melzer, Goetz Hensel, Jochen Kumlehn, Philip B Brewer, Glen R Uhrig, Marek Marzec","doi":"10.1093/pcp/pcaf095","DOIUrl":"https://doi.org/10.1093/pcp/pcaf095","url":null,"abstract":"Strigolactones (SLs) are a class of plant hormones that play a crucial role in shaping plant architecture, significantly influencing plant adaptation to harsh environmental conditions. In this study, we examined the effects of a mutation in a component of the barley SL signaling pathway, the SL repressor HvDWARF53A, on plant growth and drought tolerance. We compared the results with those of a previously described barley mutant, which is highly tillered and drought-sensitive, carrying a mutation in the SL receptor gene HvDWARF14. The two mutants, hvd14.d and hvd53a.f, displayed contrasting phenotypes, including differences in plant height, tillering, and drought sensitivity. Under control conditions, ultrastructural analysis of hvd53a.f revealed smaller chloroplasts and fewer grana stacks, which may account for its reduced photosynthetic efficiency. Conversely, transcriptomic analysis linked the differentially expressed genes in hvd53a.f to antioxidation and stress responses, suggesting a potentially enhanced capacity to cope with drought. Further analysis revealed a strong connection between the SL signaling pathway and circadian clock components. Among these, CIRCADIAN CLOCK ASSOCIATED 1 emerged as a potential SL-responsive transcription factor (TF), possibly playing a key role in regulating tillering. Under drought conditions, hvd53a.f exhibited enhanced tolerance, as evidenced by higher relative water content, reduced chlorophyll degradation, and stable, albeit reduced, photosynthetic performance. Here, we identified the SL-related TF JUNGBRUNNEN 1 as a potential regulator of genes involved in water deficit response and antioxidation processes. Overall, the hvd53a.f mutation enhances drought tolerance while maintaining low, stable photosynthesis, highlighting HvD53A as a central node connecting SL signaling to stress resilience.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144837473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Field dynamics of the root endosphere microbiome assembly in paddy rice cultivated under no fertilizer input. 无施肥条件下水稻根内微生物群组合的田间动态

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-08-12 DOI: 10.1093/pcp/pcaf045

Asahi Adachi, John Jewish Dominguez, Yuniar Devi Utami, Masako Fuji, Sumire Kirita, Shunsuke Imai, Takumi Murakami, Yuichi Hongoh, Rina Shinjo, Takehiro Kamiya, Toru Fujiwara, Kiwamu Minamisawa, Naoaki Ono, Shigehiko Kanaya, Yusuke Saijo

{"title":"Field dynamics of the root endosphere microbiome assembly in paddy rice cultivated under no fertilizer input.","authors":"Asahi Adachi, John Jewish Dominguez, Yuniar Devi Utami, Masako Fuji, Sumire Kirita, Shunsuke Imai, Takumi Murakami, Yuichi Hongoh, Rina Shinjo, Takehiro Kamiya, Toru Fujiwara, Kiwamu Minamisawa, Naoaki Ono, Shigehiko Kanaya, Yusuke Saijo","doi":"10.1093/pcp/pcaf045","DOIUrl":"10.1093/pcp/pcaf045","url":null,"abstract":"Plants accommodate diverse microbial communities, termed the microbiome, which can change dynamically during plant adaptation to varying environmental conditions. However, the direction of these changes and the underlying mechanisms driving them, particularly in crops adapting to the field conditions, are not well understood. Here, we investigate the root endosphere microbiome of rice (Oryza sativa ssp. japonica) across four consecutive cultivation seasons in a high-yield, non-fertilized, and pesticide-free paddy field, compared with a neighboring fertilized and pesticide-treated field. Using 16S rRNA amplicon and metagenome sequencing, we analyzed three Japonica cultivars-Nipponbare, Hinohikari, and Kinmaze. Our findings reveal that the root endosphere microbiomes diverge based on fertilization regime and plant developmental stages, while the effects of cultivar variation are less significant. Machine learning model and metagenomic analysis of nitrogenase (nif) genes suggest enhanced nitrogen fixation activity in the non-fertilized field-grown roots, highlighting a potential role of diazotrophic, iron-reducing bacteria Telmatospirillum. These results provide valuable insights into the assembly of the rice root microbiome in nutrient-poor soil, which can aid in managing microbial homeostasis for sustainable agriculture.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"1086-1101"},"PeriodicalIF":4.0,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144249355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome analysis of dark-adapted variants identifies the phosphatase gene phsP involved in the regulation of photosynthetic and dark-heterotrophic growth in the cyanobacterium Leptolyngbya boryana. 对暗适应变异体的基因组分析确定了磷酸酶基因phsP参与调节boryana蓝细菌的光合作用和暗异养生长。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-08-12 DOI: 10.1093/pcp/pcaf043

Shintaro Hida, Marie Nishio, Kazuma Uesaka, Mari Banba, Nobuyuki Takatani, Haruki Yamamoto, Kunio Ihara, Yuichi Fujita

{"title":"Genome analysis of dark-adapted variants identifies the phosphatase gene phsP involved in the regulation of photosynthetic and dark-heterotrophic growth in the cyanobacterium Leptolyngbya boryana.","authors":"Shintaro Hida, Marie Nishio, Kazuma Uesaka, Mari Banba, Nobuyuki Takatani, Haruki Yamamoto, Kunio Ihara, Yuichi Fujita","doi":"10.1093/pcp/pcaf043","DOIUrl":"10.1093/pcp/pcaf043","url":null,"abstract":"The prevalence of parasitic plants suggests frequent evolution of photosynthetic capacity loss in the natural environments. However, no studies have observed such evolutionary events as the loss of photosynthetic capacity. Herein, we report mutations that lead to loss or decrease in the photosynthetic growth capacity of dark-adapted variants of the cyanobacterium Leptolyngbya boryana, which can grow heterotrophically even in the dark. We isolated 28 dark-adapted variants by culturing L. boryana on agar plates containing glucose and maintained them under dark-heterotrophic conditions for 7-49 months by inoculating every 10-14 days. All variants showed significantly faster dark-heterotrophic growth than the parental strains, accompanied by the loss of photosynthetic growth capacity in 15 variants. Genome resequencing revealed that 19 of the 28 variants carried various mutations in a common single gene (LBWT_21500) encoding PP2C-type serine phosphatase. Characterization of an LBWT_21500 knockout mutant revealed that the deletion of LBWT_21500 caused a trade-off between faster dark-heterotrophic growth and slower photosynthetic growth, suggesting that the mutations in LBWT_21500 are responsible for the phenotype of 8 of the 19 variants, while other additional mutations caused the loss of photosynthetic growth capacity in the other 11 variants. Transcriptomic analysis suggested that the phosphatase is involved in the global transcriptional regulatory system that optimizes photosynthetic and dark-heterotrophic metabolism using modules of the partner switching system. We proposed the name LBWT_21500 as phototrophic-heterotrophic switching phosphatase. Mutations in global transcriptional regulatory systems may serve as one of the evolutionary steps leading to the loss of photosynthetic capacity.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"1102-1118"},"PeriodicalIF":4.0,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12344097/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxygen supply is a prerequisite for response to aluminum in cultured cells of tobacco (Nicotiana tabacum). 供氧是烟草（Nicotiana tabacum）细胞对铝反应的先决条件。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-08-12 DOI: 10.1093/pcp/pcaf055

Yoshiyuki Tsuchiya, Maki Katsuhara, Takayuki Sasaki, Yoko Yamamoto

{"title":"Oxygen supply is a prerequisite for response to aluminum in cultured cells of tobacco (Nicotiana tabacum).","authors":"Yoshiyuki Tsuchiya, Maki Katsuhara, Takayuki Sasaki, Yoko Yamamoto","doi":"10.1093/pcp/pcaf055","DOIUrl":"10.1093/pcp/pcaf055","url":null,"abstract":"Responses to aluminum (Al) were investigated in tobacco cells (cell line SL) in a calcium-sucrose solution for up to 24 h under shaking (aerobic) condition. Microarray analysis of upregulated and downregulated genes under Al exposure and following Gene Ontology (GO) enrichment analysis of biological process category revealed only one GO term to be enriched for the upregulated genes, \"response to chitin,\" annotated with genes encoding transcription factors (NtERF1 and NtMYB3) and MAP kinase (WIPK), and nine GO terms for the downregulated genes, including \"cell wall loosening\" and \"lipid transport,\" annotated with genes encoding expansin (NtEXPA4) and lipid transfer protein (LTP)/LTP-like (NtLTP3 and NtEIG-C29), respectively. Al triggered the production of nitric oxide (NO) then reactive oxygen species (ROS). Addition of NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide decreased the levels of NO and a part of the transcriptional changes described above, but increased the levels of ROS and a loss of growth capacity, suggesting a role of the NO to induce the transcriptional changes partly and to repress these toxic responses under Al exposure. Under non-shaking (anaerobic) condition, the cells exhibited upregulation of several hypoxia-responsive genes. The cells exposed to Al exhibited the same level of Al accumulation but much lower levels of the Al responses including NO production, ROS production, a loss of growth capacity, citrate secretion, and a part of the transcriptional changes described above, compared with the cells under shaking condition. These results suggest that coexistence of oxygen with Al is necessary to trigger the Al responses related to toxicity and tolerance.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"1044-1060"},"PeriodicalIF":4.0,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12344093/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144151524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondrial proteases and their roles in mitophagy in plants, animals, and yeast. 线粒体蛋白酶及其在植物、动物和酵母的线粒体自噬中的作用。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-08-12 DOI: 10.1093/pcp/pcaf038

Kacper Ludwig, Małgorzata Heidorn-Czarna

{"title":"Mitochondrial proteases and their roles in mitophagy in plants, animals, and yeast.","authors":"Kacper Ludwig, Małgorzata Heidorn-Czarna","doi":"10.1093/pcp/pcaf038","DOIUrl":"10.1093/pcp/pcaf038","url":null,"abstract":"Mitochondria play a central role in cellular respiration and other essential metabolic and signaling pathways. To function properly, mitochondria require the maintenance of proteostasis-a balance between protein synthesis and degradation. This balance is achieved through the mitochondrial protein quality control (mtPQC) system, which includes mitochondrial proteases and mitophagy. Mitochondrial proteases ensure proper protein sorting within the mitochondria and maintain proteome homeostasis by degrading unassembled, damaged, or short-lived regulatory proteins. Numerous studies have demonstrated the critical role of mitochondrial proteases in regulating mitophagy-the selective degradation of damaged, aging, or excess mitochondria or their fragments via autophagy. Notably, the rhomboid PARL protease is involved in ubiquitin-dependent PINK1-Parkin mitophagy in mammals, while the i-AAA protease Yme1 plays a role in mitophagy in budding yeast. Despite the conservation of core autophagy genes, knowledge about the molecular mechanisms and protein regulators of mitophagy in plants remains limited. In this review, we discuss recent advances in understanding the roles of mitochondrial proteases and mitophagy across plants, animals, and yeast. By comparing these mechanisms across kingdoms, we highlight the potential regulatory function of the plant i-AAA mitochondrial protease in controlling mitophagy, providing new insights into mtPQC networks in plants.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"973-990"},"PeriodicalIF":4.0,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12344103/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143978754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of salicylic acid in low CO2 response in Arabidopsis. 水杨酸在拟南芥低co2响应中的作用。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-08-12 DOI: 10.1093/pcp/pcaf052

Kosei Yoneda, Susumu Uehara, Takakazu Matsuura, Izumi C Mori, Yasuko Ito-Inaba, Takehito Inaba

{"title":"Role of salicylic acid in low CO2 response in Arabidopsis.","authors":"Kosei Yoneda, Susumu Uehara, Takakazu Matsuura, Izumi C Mori, Yasuko Ito-Inaba, Takehito Inaba","doi":"10.1093/pcp/pcaf052","DOIUrl":"10.1093/pcp/pcaf052","url":null,"abstract":"Plants use CO2 to produce carbohydrates via photosynthesis. A number of studies have focused on the high CO2 response of plants, whereas little is known about the low CO2 response of plants. Considering that phytohormones regulate responses to various environmental stresses, we hypothesized that they might also regulate the low CO2 response. In this study, we show that Arabidopsis plants adjust the low CO2 response by inducing salicylic acid (SA) and abscisic acid (ABA). Phytohormone analysis revealed a dramatic increase in SA under low CO2 conditions. Furthermore, the increase in SA was completely abolished in the SA induction-deficient2 mutant, indicating that SA is primarily synthesized via isochorismate synthase under low CO2 conditions. The level of ABA also increased under low CO2 conditions. Consistent with these observations, the expression of genes involved in SA and ABA accumulation was altered in response to low CO2 concentrations. Gene expression analysis indicated that the expression of SA- and ABA-responsive genes increased under low CO2 conditions, although the SA response of Pathogenesis-Related genes was compromised. Under low CO2 conditions, it became evident that the expression of programmed cell death-associated genes was altered, and that of photosynthesis-associated genes was suppressed. The responses of these genes were further manifested when plants accumulated high levels of SA. Taken together, we suggest that plants increase their levels of SA and ABA in response to low CO2 environments. Furthermore, the increase in SA appears to be associated with the suppression of non-essential genes and the alteration of cell death-associated genes.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"1005-1019"},"PeriodicalIF":4.0,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sulfur dioxide-induced guard cell death and stomatal closure are attenuated in nitrate/proton antiporter AtCLCa mutants. 在硝酸盐/质子反转运体AtCLCa突变体中，二氧化硫诱导的保护性细胞死亡和气孔关闭减弱。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-08-12 DOI: 10.1093/pcp/pcaf042

Lia Ooi, Takakazu Matsuura, Izumi C Mori

{"title":"Sulfur dioxide-induced guard cell death and stomatal closure are attenuated in nitrate/proton antiporter AtCLCa mutants.","authors":"Lia Ooi, Takakazu Matsuura, Izumi C Mori","doi":"10.1093/pcp/pcaf042","DOIUrl":"10.1093/pcp/pcaf042","url":null,"abstract":"Guard cells surrounding the stomata play a crucial role in regulating the entrance of hazardous gases such as SO2 into leaves. Stomatal closure could be a plant response to mitigate SO2 damage, although the mechanism for SO2-induced closure remains controversial. Proposed mediators for SO2-induced stomatal closure include phytohormones, reactive oxygen species, gasotransmitters, and cytosolic acidification. In this study, we investigated the mechanism of stomatal closure in Arabidopsis in response to SO2. Despite an increment in auxin and jasmonates after SO2 exposure, the addition of auxin did not cause stomatal closure and jasmonate-insensitive mutants exhibited SO2-induced stomatal closure suggesting auxin and jasmonates are not mediators leading to the closure. In addition, supplementation of scavenging reagents for reactive oxygen species and gasotransmitters did not inhibit SO2-induced closure. Instead, we found that cytosolic acidification is a credible mechanism for SO2-induced stomatal closure in Arabidopsis. CLCa mutants coding H+/nitrate antiporter, involved in cytosolic pH homeostasis, showed less sensitive stomatal phenotype against SO2. These results suggest that cytosolic pH homeostasis plays a tenable role in SO2 response in guard cells.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"1076-1085"},"PeriodicalIF":4.0,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12344094/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144249356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nitric oxide delayed tomato fruit coloring by regulating chlorophyll- and carotenoid-related genes in a SlSPL6c-dependent manner. 一氧化氮通过调节叶绿素延缓番茄果实着色，一氧化氮通过调节叶绿素和类胡萝卜素相关基因以依赖slspl6c的方式延缓番茄果实着色。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-08-12 DOI: 10.1093/pcp/pcaf051

Jitao Zhang, Yandong Yao, Kangding Yao, Zhiqi Ding, Wangjun Zhang, Yongjie Zhu, Wanyi Su, Weibiao Liao

{"title":"Nitric oxide delayed tomato fruit coloring by regulating chlorophyll- and carotenoid-related genes in a SlSPL6c-dependent manner.","authors":"Jitao Zhang, Yandong Yao, Kangding Yao, Zhiqi Ding, Wangjun Zhang, Yongjie Zhu, Wanyi Su, Weibiao Liao","doi":"10.1093/pcp/pcaf051","DOIUrl":"10.1093/pcp/pcaf051","url":null,"abstract":"Nitric oxide (NO) and the SQUAMOSA Promoter Binding Protein-Like family both have been shown to be involved in the fruit ripening process. This study explored the function of SlSPL6c gene in tomato (Solanum lycopersicum) fruit color change, as well as its involvement in NO-regulated coloring. The NO donor S-nitrosoglutathione (GSNO) delayed tomato fruit coloring by increasing SlSPL6c expression. The silencing of SlSPL6c accelerated tomato fruit color change, whereas overexpression of the gene delayed fruit coloring. In the SlSPL6c-silenced fruits, GSNO did not exert a significant influence on chlorophyll and carotenoid level. Whereas the chlorophyll level was significantly upregulated and carotenoid level was downregulated by GSNO in the SlSPL6c overexpression fruits. Moreover, GSNO significantly downregulated the relative expression level of the chlorophyll degradation genes (SGR1, RCCR) and carotenoid synthesis genes (PSY1) in both WT and SlSPL6c overexpression fruits; however, it did not affect the expression level of these genes in SlSPL6c silenced fruits. Thus, NO might delay tomato fruit coloring by downregulating chlorophyll degradation and carotenoid synthesis gene expression in a manner dependent on SlSPL6c. The insights gained from this study may have practical implications for the development of strategies to extend fruit shelf life in tomato and other fruit crops.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"991-1004"},"PeriodicalIF":4.0,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144094680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide SNPs data and past distribution models reveal genetic admixture and isolation in the range-restricted Psittacanthus auriculatus mistletoe species. 全基因组snp数据和过去的分布模型揭示了范围受限的Psittacanthus auriculatus槲寄生物种的遗传混合和分离。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-08-12 DOI: 10.1093/pcp/pcaf094

Juan Francisco Ornelas, Saddan Morales-Saldaña, Antonio Acini Vásquez-Aguilar

{"title":"Genome-wide SNPs data and past distribution models reveal genetic admixture and isolation in the range-restricted Psittacanthus auriculatus mistletoe species.","authors":"Juan Francisco Ornelas, Saddan Morales-Saldaña, Antonio Acini Vásquez-Aguilar","doi":"10.1093/pcp/pcaf094","DOIUrl":"https://doi.org/10.1093/pcp/pcaf094","url":null,"abstract":"The study of speciation via adaptation to different hosts is a crucial process in the evolution of parasitism. However, historical isolation and ecological factors also contribute to explaining genetic structure across space, particularly for species with restricted host ranges, alongside factors like reproductive systems, genetic drift, and mutations. Using genome-wide data, we determine the genetic structure and genetic diversity in Psittacanthus auriculatus (Loranthaceae) across its restricted geographical range in the xeric valleys surrounded by complex mountain ranges in Oaxaca. We used single nucleotide polymorphisms (SNPs) from 69 genotyped individuals of this hemiparasitic mistletoe from eight populations obtained through Genotyping by Sequencing (GBS). We used these genomic data combined with species distribution and ecological modelling and Approximate Bayesian Computation (ABC) methods, to infer the evolutionary history of P. auriculatus populations. Our analyses yielded two genetic groups, one composed of individuals from the western valleys (northern locations), and the other composed of individuals from the central valleys (southern locations) in Oaxaca, with further genetic substructure in one of the northern locations historically isolated. A scenario of habitat fragmentation during the Late Pleistocene was highly supported by species distribution modelling, in which the predicted distribution of P. auriculatus was contracted and fragmented during the Last Glacial Maximum (LGM) and expanded during the interglacials (Last Inter Glacial and present) for a more connected distribution. Our results underline historical isolation and environmental factors in moulding population genetic differentiation and structure of P. auriculatus mistletoes.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144837472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0