Plant and Cell Physiology最新文献_第8页

A synteny conserved TATA binding protein 2-associated long intergenic non-coding RNA influences leaf development and ABA response in Arabidopsis thaliana. TATA结合蛋白2相关的长基因间非编码RNA影响拟南芥叶片发育和ABA响应。

IF 4.7 2区生物学

Plant and Cell Physiology Pub Date : 2026-07-28 DOI: 10.1093/pcp/pcag040

Byanka Sthefany Espinoza-López, Jesús Nieto-Hernández, Ángel Eduardo Quezada-Eguía, Susana Isabel Vargas-Camacho, Jaime Aportela-Cortez, José Luis Reyes, Catalina Arenas-Huertero

{"title":"A synteny conserved TATA binding protein 2-associated long intergenic non-coding RNA influences leaf development and ABA response in Arabidopsis thaliana.","authors":"Byanka Sthefany Espinoza-López, Jesús Nieto-Hernández, Ángel Eduardo Quezada-Eguía, Susana Isabel Vargas-Camacho, Jaime Aportela-Cortez, José Luis Reyes, Catalina Arenas-Huertero","doi":"10.1093/pcp/pcag040","DOIUrl":"10.1093/pcp/pcag040","url":null,"abstract":"Long non-coding RNAs are molecules with regulatory potential that are involved in many biological processes in plants. Despite their significant role, only a limited number has been studied in detail. Here, we describe at the molecular and bioinformatics levels a TBP2-associated long intergenic non-coding RNA (TALIR) encoded upstream of TATA-binding protein 2 (TBP2) in Arabidopsis thaliana. Downregulation of TALIR leads to plants with reduced leaf and rosette size, as well as decreased lateral root density. The expression of TBP2 in talir mutant lines was higher than in wild-type (WT), affecting the timing of expression of miR396 and GRF2, as well as miR156 and SPL9; these dysregulations may in part contribute to the altered leaf size observed. Transcriptional evidence from the TBP2 locus suggests the presence of a putative TALIR ortholog gene, which is conserved across the Brassicaceae, Eutremeae, and Camelineae tribes at the DNA sequence and synteny levels. Furthermore, TALIR is upregulated upon ABA and NaCl treatments in WT plants, while talir mutants exhibit insensitivity to ABA during germination. Together, these findings expand current knowledge of lncRNAs and provide new insights into the regulation of miR396 and miR156 in the control of leaf growth. Further, the evolutionary conservation of TALIR among tribes within the Brassicaceae family suggests a conserved functional role in this group of plants.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"1137-1150"},"PeriodicalIF":4.7,"publicationDate":"2026-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147513892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A geometric framework for 3D leaf movement by orthonormal bases: a demonstration in Maranta leuconeura. 一种基于正交基的三维叶片运动几何框架：以白桦为例。

IF 4.7 2区生物学

Plant and Cell Physiology Pub Date : 2026-07-28 DOI: 10.1093/pcp/pcag034

Miyuki T Nakata, Shotaro Sakita, Jion Shimoyama, Naoya Ando, Masahiro Takahara

{"title":"A geometric framework for 3D leaf movement by orthonormal bases: a demonstration in Maranta leuconeura.","authors":"Miyuki T Nakata, Shotaro Sakita, Jion Shimoyama, Naoya Ando, Masahiro Takahara","doi":"10.1093/pcp/pcag034","DOIUrl":"10.1093/pcp/pcag034","url":null,"abstract":"Leaf orientation (posture) influences photosynthesis and plant responses to environmental cues. However, the existing methods for quantifying posture typically compress its inherently three-dimensional structure into scalar values or single vectors, leaving the 3D aspects of leaf movement poorly understood. For a more complete geometric description, we propose representing leaf-blade posture by an orthonormal basis (ONB), defined as three perpendicular unit vectors aligned with the developmental axes of the leaf. This ONB serves as a local coordinate system and corresponds to rotation matrices used to represent orientation in three-dimensional space, embedding leaf posture within the mathematical structure of the special orthogonal group SO(3). Using three-dimensional point-cloud data, we reconstructed an ONB aligned with the three axes of the leaf blade and quantified elevation and azimuth angles. When applied to diurnal posture changes, the resulting angular patterns were consistent with previous observations. We then visualized posture changes after gravitational perturbation as continuous rotational trajectories. These trajectories could be compared with mathematically defined geodesic shortest paths and used to simulate alternative reorientation routes that satisfy constraints. The rotational trajectories could also be separated into swing and twist components, which reflect distinct deformation modes. The ONB can be obtained not only from three-dimensional point clouds but also from other measurement tools, making the approach broadly applicable. Conceptually, ONB representation places leaf posture within the geometric structure of SO(3), enabling the use of well-established mathematical tools such as rotational distance and geodesics for analyzing leaf reorientation.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"1085-1098"},"PeriodicalIF":4.7,"publicationDate":"2026-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147444756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leaf geometry-understanding plant movement in 3D. 叶片几何-了解植物运动在三维。

IF 4.7 2区生物学

Plant and Cell Physiology Pub Date : 2026-07-28 DOI: 10.1093/pcp/pcag067

Takaaki Yonekura

引用次数: 0

The transcription factor WRKY13 activates BRUTUS to maintain iron homeostasis in Arabidopsis. 转录因子WRKY13激活BRUTUS以维持拟南芥中的铁稳态。

IF 4.7 2区生物学

Plant and Cell Physiology Pub Date : 2026-07-28 DOI: 10.1093/pcp/pcag041

Hui Song, Yifan Chen, Guangna Chen, Shuangyao Li, Shuxun Xu, Jing Zhang, Xi Wu, Shuqing Cao

{"title":"The transcription factor WRKY13 activates BRUTUS to maintain iron homeostasis in Arabidopsis.","authors":"Hui Song, Yifan Chen, Guangna Chen, Shuangyao Li, Shuxun Xu, Jing Zhang, Xi Wu, Shuqing Cao","doi":"10.1093/pcp/pcag041","DOIUrl":"10.1093/pcp/pcag041","url":null,"abstract":"Iron (Fe) is a fundamental micronutrient for plant and animal physiology. Consequently, Fe limitation in agricultural systems not only compromises crop productivity but also poses a significant threat to human nutritional security. Due to the limited bioavailability of Fe in the soil, plants have evolved adaptive strategies that can regulate Fe absorption and internal balance, thereby optimizing their survival ability under Fe-deficient conditions. BRUTUS (BTS), an Fe sensor possessing E3 ligase activity, acts as a negative regulator of the Fe deficiency response by targeting bHLH subgroup IVc transcription factors for degradation. In this study, we employed yeast one-hybrid screening to identify WRKY13, an Arabidopsis transcription factor that directly regulates BTS transcription. The expression patterns of WRKY13 and BTS exhibited similarity during the transition from Fe deficiency to Fe resupply. WRKY13 mutants display increased tolerance to Fe deficiency, whereas WRKY13 complementation lines reverse the tolerance of wrky13 mutants to wild-type levels, effectively reversing the mutant phenotype under Fe-deficient conditions. Furthermore, WRKY13 was found to directly activate BTS expression by binding to W-box motifs within the BTS promoter region. Genetic analysis positioned WRKY13 upstream of BTS within the Fe homeostasis pathway. Collectively, our findings indicate that Fe deficiency stress induces WRKY13 expression, which in turn directly activates BTS transcription to maintain cellular Fe homeostasis. In addition, upon Fe resupply, WRKY13 potentiates BTS transcriptional activation, thereby fine-tuning Fe uptake and preventing potential toxicity caused by Fe overload.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"1151-1164"},"PeriodicalIF":4.7,"publicationDate":"2026-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147581500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reconstruction of a bis(bibenzyl) biosynthetic pathway through analysis of 4-coumarate:CoA ligase and double-bond reductase in Marchantia polymorpha. 用4-香豆酸辅酶a连接酶和双键还原酶的分析重建双（联苯）生物合成途径。

IF 4.7 2区生物学

Plant and Cell Physiology Pub Date : 2026-07-28 DOI: 10.1093/pcp/pcag049

Yuka Kobayashi, Misaki Tachibana, Nagisa Kimura, Miki Hatada, Takao Koeduka, Akira Ohta, Bun-Ichi Shimizu, Taiji Nomura, Yasuo Kato, Kimitsune Ishizaki, Masaharu Mizutani, Hiroyoshi Kubo, Kojiro Takanashi

{"title":"Reconstruction of a bis(bibenzyl) biosynthetic pathway through analysis of 4-coumarate:CoA ligase and double-bond reductase in Marchantia polymorpha.","authors":"Yuka Kobayashi, Misaki Tachibana, Nagisa Kimura, Miki Hatada, Takao Koeduka, Akira Ohta, Bun-Ichi Shimizu, Taiji Nomura, Yasuo Kato, Kimitsune Ishizaki, Masaharu Mizutani, Hiroyoshi Kubo, Kojiro Takanashi","doi":"10.1093/pcp/pcag049","DOIUrl":"10.1093/pcp/pcag049","url":null,"abstract":"Marchantia polymorpha has a unique phenylpropanoid biosynthetic pathway for producing bis(bibenzyl) compounds, such as marchantins. Previous tracer studies have suggested that dihydro-p-coumaroyl-CoA, an intermediate in the M. polymorpha bis(bibenzyl) biosynthetic pathway, is derived from dihydro-p-coumaric acid, which may result from the reduction of p-coumaric acid. However, the enzymes responsible for reducing the α,β-unsaturated double bond in p-coumaric acid have not yet been identified in bryophytes or in vascular plants. To clarify the biosynthetic pathway to dihydro-p-coumaroyl-CoA in M. polymorpha, the present study characterized a 4-coumarate:CoA ligase (Mp4CL3) and a double-bond reductase (MpDBR1), both of which were upregulated in an M. polymorpha line overexpressing MpMYB2 with high accumulation of bis(bibenzyl) compounds. Enzyme assays using Escherichia coli showed that Mp4CL3 has esterification activity toward both p-coumaric acid and dihydro-p-coumaric acid with producing their corresponding CoA thioesters, whereas the reduction activity of MpDBR1 was specific to p-coumaroyl-CoA, but not to p-coumaric acid. CRISPR/Cas9-mediated knockout of MpDBR1 resulted in a significant reduction in bis(bibenzyl) content. These findings provide clear evidence for a biosynthetic route from p-coumaric acid to dihydro-p-coumaroyl-CoA via p-coumaroyl-CoA in the bis(bibenzyl) biosynthetic pathway of M. polymorpha.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"1200-1209"},"PeriodicalIF":4.7,"publicationDate":"2026-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147729776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of reactive species in modulating stilbene biosynthesis in grapevine cells. 活性物质在葡萄细胞中调节二苯乙烯合成中的作用。

IF 4.7 2区生物学

Plant and Cell Physiology Pub Date : 2026-07-28 DOI: 10.1093/pcp/pcag033

Eduardo Gómez-Copoví, Ana Belén Sabater-Jara, José Manuel Correa-Sabater, Maria A Pedreño, Lorena Almagro

{"title":"The role of reactive species in modulating stilbene biosynthesis in grapevine cells.","authors":"Eduardo Gómez-Copoví, Ana Belén Sabater-Jara, José Manuel Correa-Sabater, Maria A Pedreño, Lorena Almagro","doi":"10.1093/pcp/pcag033","DOIUrl":"10.1093/pcp/pcag033","url":null,"abstract":"Stilbene trans-resveratrol is a key specialized metabolite in grapevine (Vitis vinifera), known for its notable antioxidant, anti-inflammatory, and anticancer properties. Its biosynthesis is primarily induced under stress conditions and can be enhanced in grapevine cell cultures using elicitors such as cyclodextrins and methyl jasmonate. However, the involvement of reactive species in this process remains poorly understood. In this study, we investigated the roles of hydrogen peroxide, nitric oxide, peroxynitrite, and hydrogen sulfide in trans-resveratrol production in grapevine suspension-cultured cells treated with cyclodextrins and/or methyl jasmonate. The exogenous application of donors and scavengers/inhibitors of these reactive species revealed differential effects on trans-resveratrol accumulation. Nitric oxide and hydrogen sulfide donors significantly increased trans-resveratrol levels, whereas the use of specific scavengers reduced its production. In contrast, the addition of peroxynitrite or hydrogen peroxide donors had no significant effect, although their scavengers decreased trans-resveratrol accumulation. Moreover, confocal microscopy analyses showed that elicitation with cyclodextrins and methyl jasmonate modulated the intracellular levels of these reactive species over time. Our findings demonstrate that the balance of hydrogen peroxide, nitric oxide, peroxynitrite, and hydrogen sulfide is regulated during elicitation and plays a crucial role in modulating trans-resveratrol biosynthesis.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"1075-1084"},"PeriodicalIF":4.7,"publicationDate":"2026-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147390754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RHE1 is associated with short-day-induced hypocotyl elongation, peroxisomal ROS homeostasis, and m 6A-dependent RNA stability in Arabidopsis. 在拟南芥中，RHE1与短日照诱导的下胚轴伸长、过氧化物酶体ROS稳态和m6a依赖性RNA稳定性有关。

IF 4.7 2区生物学

Plant and Cell Physiology Pub Date : 2026-07-28 DOI: 10.1093/pcp/pcag105

Yibin Lin, Yijun Meng, Hidetaka Ito

{"title":"RHE1 is associated with short-day-induced hypocotyl elongation, peroxisomal ROS homeostasis, and m 6A-dependent RNA stability in Arabidopsis.","authors":"Yibin Lin, Yijun Meng, Hidetaka Ito","doi":"10.1093/pcp/pcag105","DOIUrl":"https://doi.org/10.1093/pcp/pcag105","url":null,"abstract":"Plants dynamically adjust growth in response to short-day (SD) conditions while maintaining reactive oxygen species (ROS) homeostasis across cellular compartments. However, how light signaling is coupled to organelle-specific redox regulation remains poorly understood. Here, we identify the peroxisomal Mpv17/PMP22 family protein REGULATOR OF ROS-MEDIATED HYPOCOTYL ELONGATION 1 (RHE1) as a regulator of short-day-induced hypocotyl elongation and ROS homeostasis in Arabidopsis thaliana. RHE1 expression is associated with a light-responsive G-box element bound by HY5 and shows a strong positive relationship with N6-methyladenosine (m6A) enrichment. Environmental stresses that elevate inferred m6A levels increase RHE1 transcript abundance and stability, whereas short-day conditions reduce m6A enrichment and decrease RHE1 expression. Loss of RHE1 results in enhanced hypocotyl cell elongation and elevated ROS accumulation specifically under short-day conditions, indicating that RHE1 constrains growth through peroxisomal redox regulation. Furthermore, RHE1 transcript levels correlate with the m6A reader ECT2, and published binding datasets support direct association of ECT2 with RHE1 mRNA. Together, these findings reveal a multilayered regulatory mechanism in which HY5-associated transcription and m6A-dependent RNA stabilization converge on RHE1 to coordinate SD-dependent growth with peroxisomal ROS homeostasis. This study uncovers a previously unrecognized connection between SD signaling, epitranscriptomic regulation, and organelle-specific redox control.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148606930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arabidopsis SWEET12 contributes to the regulation of sugar allocation and defense responses during interaction with Serendipita indica. 拟南芥与Serendipita相互作用时，SWEET12参与糖分配和防御反应的调控。

IF 4.7 2区生物学

Plant and Cell Physiology Pub Date : 2026-07-28 DOI: 10.1093/pcp/pcag032

Abhimanyu Jogawat, Madhan Sanyasi, Sidhardh H Menon, Divya Goyal, Athira Mohandas Nair, Jyothilakshmi Vadassery

{"title":"Arabidopsis SWEET12 contributes to the regulation of sugar allocation and defense responses during interaction with Serendipita indica.","authors":"Abhimanyu Jogawat, Madhan Sanyasi, Sidhardh H Menon, Divya Goyal, Athira Mohandas Nair, Jyothilakshmi Vadassery","doi":"10.1093/pcp/pcag032","DOIUrl":"10.1093/pcp/pcag032","url":null,"abstract":"Carbon availability is a central determinant of beneficial plant-fungal associations, and sugar transporters are key levers of this exchange. SWEETs (SUGARS WILL EVENTUALLY BE EXPORTED TRANSPORTER) are involved in transporting various kinds of sugars in plants; however, their functional roles in fungal symbiosis are not sufficiently explored. In this study, we investigate the functional relevance of Arabidopsis SWEETs in the interaction with the endophytic fungi, Serendipita indica. Transcript profiling of SWEET genes in response to S. indica and its major elicitor, cellotriose, revealed the early root-specific induction of SWEET12. Using a SWEET12 loss-of-function mutant, we demonstrate that the absence of SWEET12 disrupts the major outcomes of mutualism, including growth promotion, balanced colonization, sugar allocation, and the accumulation of defense phytohormones (jasmonic acid and salicylic acid). Transcriptome profiling further reveals that SWEET12 buffers whole-plant responses by coordinating genes linked to carbohydrate, nitrogen, and lipid metabolism, and by tuning defense signaling and nutrient transporter networks. Our findings indicate that SWEET12 is essential for balancing fungal colonization and host defense, thereby promoting plant growth. SWEET12 does so by acting as a sugar valve that meters sugar release to the apoplast, enabling the fungus to access carbon while preserving host sugar homeostasis and immune competence.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"1056-1074"},"PeriodicalIF":4.7,"publicationDate":"2026-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147444767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of a cytosol-localized dual prenyl diphosphate synthase involved in monoterpene and sesquiterpene biosynthesis in strawberry. 参与草莓单萜和倍半萜合成的胞质定位双戊烯基二磷酸合成酶的鉴定。

IF 4.7 2区生物学

Plant and Cell Physiology Pub Date : 2026-07-28 DOI: 10.1093/pcp/pcag051

Yoriyuki Tachibana, Hayato Ueoka, Momoka Goto, Nanako Dan, Sora Koita, Hao Li, Hiroaki Kusano, Takuji Ichino, Ryosuke Munakata, Akifumi Sugiyama, Kazufumi Yazaki

{"title":"Characterization of a cytosol-localized dual prenyl diphosphate synthase involved in monoterpene and sesquiterpene biosynthesis in strawberry.","authors":"Yoriyuki Tachibana, Hayato Ueoka, Momoka Goto, Nanako Dan, Sora Koita, Hao Li, Hiroaki Kusano, Takuji Ichino, Ryosuke Munakata, Akifumi Sugiyama, Kazufumi Yazaki","doi":"10.1093/pcp/pcag051","DOIUrl":"10.1093/pcp/pcag051","url":null,"abstract":"Plants produce more than a million metabolites, most of which are compounds from specialized metabolism. The biosynthesis of these metabolites is controlled by different organelles within cells. For terpenoids in particular, plant cells have two independent pathways: the methylerythritol phosphate (MEP) pathway in plastids and the mevalonate (MVA) pathway in the cytosol. Monoterpenes, a class of C10 compounds, play important roles in plant communication with other organisms, such as pollinators and herbivores, to aid reproduction and deter predators, respectively. Monoterpenes are generally produced by terpene synthases (TPSs), which are responsible for producing each end product from the common substrate, geranyl diphosphate (GPP), via the MEP pathway in plastids, where GPP biosynthesis also occurs. However, linalool, a major monoterpene found in strawberries (Fragaria × ananassa), is synthesized via the MVA pathway in the cytosol by a cytosol-localized TPS, named FaNES1. The enzyme that provides its substrate remains unknown. In this study, we identified two strong candidates for cytosol-localized trans-prenyltransferases. Both candidates were functionally characterized, and we demonstrated that one yielded both GPP and farnesyl diphosphate (FPP), with a higher efficiency for GPP. The other protein exclusively yielded FPP. Both proteins lack a transit peptide and are localized in the cytosol. Gene expression analysis revealed that the GPP/FPP synthase is almost constantly expressed in strawberries. We also discuss the putative molecular evolution of the unique strawberry GPP synthase.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"1225-1236"},"PeriodicalIF":4.7,"publicationDate":"2026-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147729784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Manipulating the Gibberellin Pathway Alters Harvest Index and Cannabinoid Content in Cannabis sativa L. 调控赤霉素通路改变大麻收获指数和大麻素含量。

IF 4.7 2区生物学

Plant and Cell Physiology Pub Date : 2026-07-26 DOI: 10.1093/pcp/pcag104

Lennard Garcia-de Heer, Matthew Nolan, Jos Mieog, Qi Guo, Nicolas Dimopoulos, Lei Liu, Xuan Chen, Tobias Kretzschmar

{"title":"Manipulating the Gibberellin Pathway Alters Harvest Index and Cannabinoid Content in Cannabis sativa L.","authors":"Lennard Garcia-de Heer, Matthew Nolan, Jos Mieog, Qi Guo, Nicolas Dimopoulos, Lei Liu, Xuan Chen, Tobias Kretzschmar","doi":"10.1093/pcp/pcag104","DOIUrl":"https://doi.org/10.1093/pcp/pcag104","url":null,"abstract":"Cannabis sativa is a multi-purpose crop with a wide range of industrial and medicinal end uses. Despite its long history of cultivation, it has not received the same breeding efforts as many other crops due to regulatory barriers. In particular, it bypassed the Green Revolution, which improved harvest indices of crops via developing semi-dwarf varieties by manipulating endogenous gibberellin signalling pathways. We chemically manipulated gibberellin signalling through the exogenous application of gibberellic acid and the gibberellin biosynthesis inhibitor paclobutrazol. We used multi-omic analysis to determine the effect of these applications on the proteome, transcriptome and secondary metabolite accumulation to assess the potential of gibberellin modulation for improved C. sativa end uses. Paclobutrazol treatments resulted in a semi-dwarf phenotype with an improved high harvest index and enhanced cannabinoid accumulation. Differentially expressed gene and weighted gene co-expression network analysis revealed the importance of source/sink dynamics for harvest index, with the increased expression of sucrose synthases and sugar transporters key to the phenotypic changes induced by gibberellin modulation. At the same time, the availability of hexanoate precursors via the degradation of long-chain fatty acids emerged as a likely constraint on cannabinoid accumulation. This study provides a basis for exploring the manipulation of gibberellin pathways in C. sativa varieties and their relevance to cannabinoid production.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148593038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0