Plant and Cell Physiology最新文献_第8页

Plant and Algal Lipids: In All Their States and on All Scales. 植物和藻类脂质：各种状态和各种规模。

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2024-06-27 DOI: 10.1093/pcp/pcae061

Yonghua Li-Beisson, Rebecca L Roston

引用次数: 0

Characterization of Unique Eukaryotic Sphingolipids with Temperature-Dependent Δ8-Unsaturation from the Picoalga Ostreococcus tauri. 具有温度依赖性Δ8-不饱和度的独特真核鞘磷脂的特征，来自皮卡鱼 Ostreococcus Tauri。

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2024-06-27 DOI: 10.1093/pcp/pcae007

Toshiki Ishikawa, Frédéric Domergue, Alberto Amato, Florence Corellou

{"title":"Characterization of Unique Eukaryotic Sphingolipids with Temperature-Dependent Δ8-Unsaturation from the Picoalga Ostreococcus tauri.","authors":"Toshiki Ishikawa, Frédéric Domergue, Alberto Amato, Florence Corellou","doi":"10.1093/pcp/pcae007","DOIUrl":"10.1093/pcp/pcae007","url":null,"abstract":"Sphingolipids (SLs) are ubiquitous components of eukaryotic cell membranes and are found in some prokaryotic organisms and viruses. They are composed of a sphingoid backbone that may be acylated and glycosylated. Assembly of various sphingoid base, fatty acyl and glycosyl moieties results in highly diverse structures. The functional significance of variations in SL chemical diversity and abundance is still in the early stages of investigation. Among SL modifications, Δ8-desaturation of the sphingoid base occurs only in plants and fungi. In plants, SL Δ8-unsaturation is involved in cold hardiness. Our knowledge of the structure and functions of SLs in microalgae lags far behind that of animals, plants and fungi. Original SL structures have been reported from microalgae. However, functional studies are still missing. Ostreococcus tauri is a minimal microalga at the base of the green lineage and is therefore a key organism for understanding lipid evolution. In the present work, we achieved the detailed characterization of O. tauri SLs and unveiled unique glycosylceramides as sole complex SLs. The head groups are reminiscent of bacterial SLs, as they contain hexuronic acid residues and can be polyglycosylated. Ceramide backbones show a limited variety, and SL modification is restricted to Δ8-unsaturation. The Δ8-SL desaturase from O. tauri only produced E isomers. Expression of both Δ8-SL desaturase and Δ8-unsaturation of sphingolipids varied with temperature, with lower levels at 24°C than at 14°C. Overexpression of the Δ8-SL desaturase dramatically increases the level of Δ8 unsaturation at 24°C and is paralleled by a failure to increase cell size. Our work provides the first characterization of O. tauri SLs and functional evidence for the involvement of SL Δ8-unsaturation for temperature acclimation in microalgae, suggesting that this function is an ancestral feature in the green lineage.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139513325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Post-translational Regulation of BRI1-EMS Suppressor 1 and Brassinazole-resistant 1. BRI1-EMS 抑制因子 1 和抗黄铜唑因子 1 的翻译后调控。

IF 4.9 2区生物学

Plant and Cell Physiology Pub Date : 2024-06-19 DOI: 10.1093/pcp/pcae066

Juan Mao, Biaodi Shen, Wenxin Li, Linchuan Liu, Jianming Li

引用次数: 0

Letter to the Editor: Removal of B800 Bacteriochlorophyll a from Light-Harvesting Complex 3 of the Purple Photosynthetic Bacterium Rhodoblastus acidophilus. 致编辑的信：从紫色光合细菌嗜酸乳杆菌的光收集复合体 3 中去除 B800 菌体叶绿素 a。

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2024-06-11 DOI: 10.1093/pcp/pcae065

Yoshitaka Saga, Kohei Hamanishi, Shota Kawato

引用次数: 0

Light Wavelength as a Contributory Factor of Environmental Fitness in the Cyanobacterial Circadian Clock. 光波长是蓝藻昼夜节律钟环境适应性的一个促成因素。

IF 4.9 2区生物学

Plant and Cell Physiology Pub Date : 2024-05-30 DOI: 10.1093/pcp/pcae022

Naohiro Kawamoto, Shuji Nakanishi, Ginga Shimakawa

{"title":"Light Wavelength as a Contributory Factor of Environmental Fitness in the Cyanobacterial Circadian Clock.","authors":"Naohiro Kawamoto, Shuji Nakanishi, Ginga Shimakawa","doi":"10.1093/pcp/pcae022","DOIUrl":"10.1093/pcp/pcae022","url":null,"abstract":"A circadian clock is an essential system that drives the 24-h expression rhythms for adaptation to day-night cycles. The molecular mechanism of the circadian clock has been extensively studied in cyanobacteria harboring the KaiC-based timing system. Nevertheless, our understanding of the physiological significance of the cyanobacterial circadian clock is still limited. In this study, we cultured wild-type Synechococcus elongatus PCC 7942 and circadian clock mutants in day-night cycles at different light qualities and found that the growth of the circadian clock mutants was specifically impaired during 12-h blue light/12-h dark (BD) cycles for the first time. The arrhythmic mutant kaiCAA was further analyzed by photosynthetic measurements. Compared with the wild type, the mutant exhibited decreases in the chlorophyll content, the ratio of photosystem I to II, net O2 evolution rate and efficiency of photosystem II photochemistry during BD cycles. These results indicate that the circadian clock is necessary for the growth and the maintenance of the optimum function of the photosynthetic apparatus in cyanobacteria under blue photoperiodic conditions.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140028742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA- and Selectable-Marker-Free Genome-Editing System Using Zygotes from Recalcitrant Maize Inbred B73. 利用难育玉米近交系 B73 的子代进行 DNA 和无选择标记基因组编辑的系统。

IF 4.9 2区生物学

Plant and Cell Physiology Pub Date : 2024-05-30 DOI: 10.1093/pcp/pcae010

Hajime Yamada, Norio Kato, Masako Ichikawa, Keiko Mannen, Takatoshi Kiba, Yuriko Osakabe, Hitoshi Sakakibara, Minami Matsui, Takashi Okamoto

{"title":"DNA- and Selectable-Marker-Free Genome-Editing System Using Zygotes from Recalcitrant Maize Inbred B73.","authors":"Hajime Yamada, Norio Kato, Masako Ichikawa, Keiko Mannen, Takatoshi Kiba, Yuriko Osakabe, Hitoshi Sakakibara, Minami Matsui, Takashi Okamoto","doi":"10.1093/pcp/pcae010","DOIUrl":"10.1093/pcp/pcae010","url":null,"abstract":"Genome-editing tools such as the clustered regularly interspaced short palindromic repeats/Cas9 (CRISPR/Cas9) system have become essential tools for increasing the efficiency and accuracy of plant breeding. Using such genome-editing tools on maize, one of the most important cereal crops of the world, will greatly benefit the agriculture and the mankind. Conventional genome-editing methods typically used for maize involve insertion of a Cas9-guide RNA expression cassette and a selectable marker in the genome DNA; however, using such methods, it is essential to eliminate the inserted DNA cassettes to avoid legislative concerns on gene-modified organisms. Another major hurdle for establishing an efficient and broadly applicable DNA-free genome-editing system for maize is presented by recalcitrant genotypes/cultivars, since cell/tissue culture and its subsequent regeneration into plantlets are crucial for producing transgenic and/or genome-edited maize. In this study, to establish a DNA-free genome-editing system for recalcitrant maize genotypes/cultivars, Cas9-gRNA ribonucleoproteins were directly delivered into zygotes isolated from the pollinated flowers of the maize-B73 cultivar. The zygotes successfully developed and were regenerated into genome-edited plantlets by co-culture with phytosulfokine, a peptide phytohormone. The method developed herein made it possible to obtain DNA- and selectable-marker-free genome-edited recalcitrant maize genotypes/cultivars with high efficiency. This method can advance the molecular breeding of maize and other important cereals, regardless of their recalcitrant characteristics.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139575936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trans-Species Mobility of RNA Interference between Plants and Associated Organisms. 植物和相关生物之间 RNA 干扰的跨物种流动性。

IF 4.9 2区生物学

Plant and Cell Physiology Pub Date : 2024-05-30 DOI: 10.1093/pcp/pcae012

Ya-Chi Nien, Allison Vanek, Michael J Axtell

引用次数: 0

Divergent Protein Redox Dynamics and Their Relationship with Electron Transport Efficiency during Photosynthesis Induction. 光合作用诱导过程中不同蛋白质的氧化还原动力学及其与电子传递效率的关系

IF 4.9 2区生物学

Plant and Cell Physiology Pub Date : 2024-05-30 DOI: 10.1093/pcp/pcae013

Keisuke Yoshida, Toru Hisabori

{"title":"Divergent Protein Redox Dynamics and Their Relationship with Electron Transport Efficiency during Photosynthesis Induction.","authors":"Keisuke Yoshida, Toru Hisabori","doi":"10.1093/pcp/pcae013","DOIUrl":"10.1093/pcp/pcae013","url":null,"abstract":"Various chloroplast proteins are activated/deactivated during the light/dark cycle via the redox regulation system. Although the photosynthetic electron transport chain provides reducing power to redox-sensitive proteins via the ferredoxin (Fd)/thioredoxin (Trx) pathway for their enzymatic activity control, how the redox states of individual proteins are linked to electron transport efficiency remains uncharacterized. Here we addressed this subject with a focus on the photosynthetic induction phase. We used Arabidopsis plants, in which the amount of Fd-Trx reductase (FTR), a core component in the Fd/Trx pathway, was genetically altered. Several chloroplast proteins showed different redox shift responses toward low- and high-light treatments. The light-dependent reduction of Calvin-Benson cycle enzymes fructose 1,6-bisphosphatase (FBPase) and sedoheptulose 1,7-bisphosphatase (SBPase) was partially impaired in the FTR-knockdown ftrb mutant. Simultaneous analyses of chlorophyll fluorescence and P700 absorbance change indicated that the induction of the electron transport reactions was delayed in the ftrb mutant. FTR overexpression also mildly affected the reduction patterns of FBPase and SBPase under high-light conditions, which were accompanied by the modification of electron transport properties. Accordingly, the redox states of FBPase and SBPase were linearly correlated with electron transport rates. In contrast, ATP synthase was highly reduced even when electron transport reactions were not fully induced. Furthermore, the redox response of proton gradient regulation 5-like photosynthetic phenotype1 (PGRL1; a protein involved in cyclic electron transport) did not correlate with electron transport rates. Our results provide insights into the working dynamics of the redox regulation system and their differential associations with photosynthetic electron transport efficiency.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11138366/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139672527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-Term Consequences of PTI Activation and Its Manipulation by Root-Associated Microbiota. 根相关微生物群对 PTI 激活及其操纵的长期影响。

IF 4.9 2区生物学

Plant and Cell Physiology Pub Date : 2024-05-30 DOI: 10.1093/pcp/pcae033

Ryohei Thomas Nakano, Tomohisa Shimasaki

{"title":"Long-Term Consequences of PTI Activation and Its Manipulation by Root-Associated Microbiota.","authors":"Ryohei Thomas Nakano, Tomohisa Shimasaki","doi":"10.1093/pcp/pcae033","DOIUrl":"10.1093/pcp/pcae033","url":null,"abstract":"In nature, plants are constantly colonized by a massive diversity of microbes engaged in mutualistic, pathogenic or commensal relationships with the host. Molecular patterns present in these microbes activate pattern-triggered immunity (PTI), which detects microbes in the apoplast or at the tissue surface. Whether and how PTI distinguishes among soil-borne pathogens, opportunistic pathogens, and commensal microbes within the soil microbiota remains unclear. PTI is a multimodal series of molecular events initiated by pattern perception, such as Ca2+ influx, reactive oxygen burst, and extensive transcriptional and metabolic reprogramming. These short-term responses may manifest within minutes to hours, while the long-term consequences of chronic PTI activation persist for days to weeks. Chronic activation of PTI is detrimental to plant growth, so plants need to coordinate growth and defense depending on the surrounding biotic and abiotic environments. Recent studies have demonstrated that root-associated commensal microbes can activate or suppress immune responses to variable extents, clearly pointing to the role of PTI in root-microbiota interactions. However, the molecular mechanisms by which root commensals interfere with root immunity and root immunity modulates microbial behavior remain largely elusive. Here, with a focus on the difference between short-term and long-term PTI responses, we summarize what is known about microbial interference with host PTI, especially in the context of root microbiota. We emphasize some missing pieces that remain to be characterized to promote the ultimate understanding of the role of plant immunity in root-microbiota interactions.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140319072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High Myristic Acid in Glycerolipids Enhances the Repair of Photodamaged Photosystem II under Strong Light. 甘油三酯中的高肉豆蔻酸可增强强光下光损光系统 II 的修复能力。

IF 4.9 2区生物学

Plant and Cell Physiology Pub Date : 2024-05-30 DOI: 10.1093/pcp/pcae021

Kazuki Kurima, Haruhiko Jimbo, Takashi Fujihara, Masakazu Saito, Toshiki Ishikawa, Hajime Wada

{"title":"High Myristic Acid in Glycerolipids Enhances the Repair of Photodamaged Photosystem II under Strong Light.","authors":"Kazuki Kurima, Haruhiko Jimbo, Takashi Fujihara, Masakazu Saito, Toshiki Ishikawa, Hajime Wada","doi":"10.1093/pcp/pcae021","DOIUrl":"10.1093/pcp/pcae021","url":null,"abstract":"Cyanobacteria inhabit areas with a broad range of light, temperature and nutrient conditions. The robustness of cyanobacterial cells, which can survive under different conditions, may depend on the resilience of photosynthetic activity. Cyanothece sp. PCC 8801 (Cyanothece), a freshwater cyanobacterium isolated from a Taiwanese rice field, had a higher repair activity of photodamaged photosystem II (PSII) under intense light than Synechocystis sp. PCC 6803 (Synechocystis), another freshwater cyanobacterium. Cyanothece contains myristic acid (14:0) as the major fatty acid at the sn-2 position of the glycerolipids. To investigate the role of 14:0 in the repair of photodamaged PSII, we used a Synechocystis transformant expressing a T-1274 encoding a lysophosphatidic acid acyltransferase (LPAAT) from Cyanothece. The wild-type and transformant cells contained 0.2 and 20.1 mol% of 14:0 in glycerolipids, respectively. The higher content of 14:0 in the transformants increased the fluidity of the thylakoid membrane. In the transformants, PSII repair was accelerated due to an enhancement in the de novo synthesis of D1 protein, and the production of singlet oxygen (1O2), which inhibited protein synthesis, was suppressed. The high content of 14:0 increased transfer of light energy received by phycobilisomes to PSI and CP47 in PSII and the content of carotenoids. These results indicated that an increase in 14:0 reduced 1O2 formation and enhanced PSII repair. The higher content of 14:0 in the glycerolipids may be required as a survival strategy for Cyanothece inhabiting a rice field under direct sunlight.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11138363/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140028741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0