Plant and Cell Physiology最新文献_第10页

Identification and Transcription Analysis of Phenolic Acid Biosynthesis Pathway in Pseudotaxus chienii. 伪红豆杉酚酸生物合成途径的鉴定与转录分析。

IF 4.7 2区生物学

Plant and Cell Physiology Pub Date : 2026-07-16 DOI: 10.1093/pcp/pcag097

Xiaori Zhan, Zijing Zhou, Yuting Peng, Xinru Wei, Zixin Shao, Yanheng Hu, Yanjun Yang, Chenjia Shen

{"title":"Identification and Transcription Analysis of Phenolic Acid Biosynthesis Pathway in Pseudotaxus chienii.","authors":"Xiaori Zhan, Zijing Zhou, Yuting Peng, Xinru Wei, Zixin Shao, Yanheng Hu, Yanjun Yang, Chenjia Shen","doi":"10.1093/pcp/pcag097","DOIUrl":"https://doi.org/10.1093/pcp/pcag097","url":null,"abstract":"Pseudotaxus chienii, an endangered relic conifer endemic to China, possesses significant medicinal potential, yet its phenolic acid biosynthesis and glycosylation mechanisms remain unelucidated. This study integrated metabolomics, mass spectrometry imaging (MSI), transcriptomics, and molecular biology to systematically characterize the phenolic acid pathway in P. chienii. Untargeted metabolomics identified distinct phenolic acid accumulation patterns between P. chienii and Taxus mairei. MALDI-2 MSI visualized ten phenolic acids, confirming leaves as the primary accumulation site. Genome-wide analysis revealed a complete phenolic acid biosynthesis pathway with key enzyme-encoding genes (4 PAL, 5 CCR, 2 F5H, etc.), while HQT was absent, indicating loss of chlorogenic acid synthesis. We identified 504 glycosyltransferase (GT) genes, with 48 leaf-specific ones (predominantly GT1 subfamily). Promoter and correlation analyses highlighted WRKY (PichiChr12G332970.1) and ERF (PichiChr3G086100.1) as core transcription factors (TFs). EMSA and dual-luciferase assays validated WRKY(PichiChr12G332970.1) directly activating three GT genes. This study clarifies the molecular basis and transcriptional regulation of phenolic acid glycosylation in P. chienii, providing a framework for exploiting its medicinal resources and guiding conservation-oriented breeding.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148472474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Co-option of developmental receptor-ligand signalling in plant symbiosis. 植物共生中发育受体-配体信号的共选择。

IF 4.7 2区生物学

Plant and Cell Physiology Pub Date : 2026-07-16 DOI: 10.1093/pcp/pcag094

Álvaro Daniel Fernández-Fernández

引用次数: 0

The extracellular regulatory network and key factors governing early embryogenesis in Nicotiana tabacum. 烟草早期胚胎发生的细胞外调控网络及关键因素。

IF 4.7 2区生物学

Plant and Cell Physiology Pub Date : 2026-07-08 DOI: 10.1093/pcp/pcag095

An Luo, Ying Qiao, Siyuan Li, Xinyue Wang, Ce Shi, Pan Luo

{"title":"The extracellular regulatory network and key factors governing early embryogenesis in Nicotiana tabacum.","authors":"An Luo, Ying Qiao, Siyuan Li, Xinyue Wang, Ce Shi, Pan Luo","doi":"10.1093/pcp/pcag095","DOIUrl":"https://doi.org/10.1093/pcp/pcag095","url":null,"abstract":"The plant extracellular space, including the cell wall, is a dynamic signaling compartment outside the plasma membrane that plays crucial roles in cell-cell communication and developmental coordination. Although early embryogenesis is known to be orchestrated by both intracellular and extracellular cues, the molecular mechanisms underlying extracellular regulation remain largely unexplored. Here, we report that NtProRP1, an extracellular protein, localizes to the cell wall immediately after fertilization in Nicotiana tabacum. Targeted knockout of NtProRP1 using the CRISPR-Cas9 system resulted in arrested embryos with irregular cell shapes and aberrant division patterns in aborted seeds. Furthermore, we performed high-resolution transcriptome profiling on a limited number of isolated two-celled proembryos from wild-type (WT) and Ntprorp1 plants, enabling the detection of early transcriptional changes with minimal interference from surrounding tissues. Genes involved in the synthesis and modification of cell wall components, including cellulose, hemicellulose, and pectin, were coordinately upregulated at the onset of embryogenesis in the Ntprorp1 mutant. Further analysis suggests a possible role for NtProRP1 in cell wall formation via the cell wall integrity (CWI) maintenance mechanism, potentially through the involvement of components such as receptor-like kinases (RLKs), mitogen-activated protein kinases (MAPKs), Ca2+ flux, and multiple phytohormones. Together, these results uncover a new mechanism for NtProRP1 in regulating early embryogenesis and deliver a unique transcriptomic resource that advances understanding of extracellular signaling in plant embryogenesis.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148405513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Flexible, Top-Down Approach To Assays Of Leaf Movement Rhythms. 一个灵活的，自上而下的方法来分析叶片运动节奏。

IF 4.7 2区生物学

Plant and Cell Physiology Pub Date : 2026-07-04 DOI: 10.1093/pcp/pcag091

Ann Feke, Eva M Farré

引用次数: 0

Alternative Splicing Fine-Tunes RVE8 Function in Plant Growth Regulation. 选择性剪接微调RVE8在植物生长调控中的作用

IF 4.7 2区生物学

Plant and Cell Physiology Pub Date : 2026-07-04 DOI: 10.1093/pcp/pcag092

Constanza H Assaf, Santiago Mora García, Marcelo J Yanovsky

引用次数: 0

The calmodulin-like proteins, CML13 and CML14 function as myosin light chains for the class XI myosins in Arabidopsis. 钙调素样蛋白CML13和CML14在拟南芥中作为XI类肌球蛋白轻链起作用。

IF 4.7 2区生物学

Plant and Cell Physiology Pub Date : 2026-06-30 DOI: 10.1093/pcp/pcag012

Kyle Symonds, Liam Duff, Vikas Dwivedi, Eduard Belausov, Lalita Pal, Motoki Tominaga, Takeshi Haraguchi, Einat Sadot, Kohji Ito, Wayne A Snedden

{"title":"The calmodulin-like proteins, CML13 and CML14 function as myosin light chains for the class XI myosins in Arabidopsis.","authors":"Kyle Symonds, Liam Duff, Vikas Dwivedi, Eduard Belausov, Lalita Pal, Motoki Tominaga, Takeshi Haraguchi, Einat Sadot, Kohji Ito, Wayne A Snedden","doi":"10.1093/pcp/pcag012","DOIUrl":"10.1093/pcp/pcag012","url":null,"abstract":"Myosins are crucial motor proteins associated with the actin cytoskeleton in eukaryotic cells. Structurally, myosins form heteromeric complexes, with smaller light chains such as calmodulin (CaM) bound to isoleucine-glutamine (IQ) domains in the neck region. These interactions facilitate mechano-enzymatic activity. Recently, we reported that Arabidopsis CaM-like (CML) proteins CML13 and CML14 interact with the IQ domains of various proteins and function as myosin VIII light chains. Here, we demonstrate that CaM, CML13, and CML14 specifically bind to the neck region of all 13 Arabidopsis myosin XI isoforms, with some specificity among the CaM/CML-IQ domains. We observed distinct residue preferences within the Myo XI IQ domains for CML13, CML14, and CaM. Recombinant CaM, CML13, and CML14 exhibited calcium-insensitive binding to the IQ domains of myosin XIs. CaM, CML13, and CML14 co-localized to microtubules when co-expressed with MAP65-1-myosin fusion proteins containing the IQ domains of myosin XIs. In addition, in vitro actin motility assays demonstrated that CML13, CML14, and CaM function as myosin XI light chains. A cml13 T-DNA mutant exhibited a shortened primary root phenotype that was complemented by the wild-type CML13 and was similar to that observed in a triple myosin XI mutant (xi-1,2,k). Overall, our data indicate that Arabidopsis CML13 and CML14 are novel myosin XI light chains that likely participate in various myosin XI functions.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"917-927"},"PeriodicalIF":4.7,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13365142/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146107129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Subfamily-level comparative transcriptomics of key immune regulators in plants and suspension cells reveals novel rice blast resistance genes. 植物和悬浮细胞中关键免疫调节因子的亚家族水平比较转录组学揭示了新的水稻抗稻瘟病基因。

IF 4.7 2区生物学

Plant and Cell Physiology Pub Date : 2026-06-30 DOI: 10.1093/pcp/pcag019

Wanqing Wang, Fumi Fukada, Tomoyuki Furuta, Alfino Sebastian, Kiwamu Hyodo, Natsuko Ono, Hideki Nishimura, Pingyu Wang, Yoji Kawano

{"title":"Subfamily-level comparative transcriptomics of key immune regulators in plants and suspension cells reveals novel rice blast resistance genes.","authors":"Wanqing Wang, Fumi Fukada, Tomoyuki Furuta, Alfino Sebastian, Kiwamu Hyodo, Natsuko Ono, Hideki Nishimura, Pingyu Wang, Yoji Kawano","doi":"10.1093/pcp/pcag019","DOIUrl":"10.1093/pcp/pcag019","url":null,"abstract":"Plants activate pattern-triggered immunity through key immune components, including pattern recognition receptors (PRRs), receptor-like cytoplasmic kinases (RLCKs), and transcription factors (TFs), to combat pathogens. However, a comprehensive transcriptional overview of these immune regulators at the subfamily level during biotic stress in rice is currently lacking. The aims of this study were to characterize the expression profiles of Oryza sativa (Os)PRRs, OsRLCKs, and OsTFs, and establish a robust pipeline for selecting novel candidate genes involved in plant immunity. We identified differentially expressed genes (DEGs) within these families using transcriptomic data from both rice plants infected with Magnaporthe oryzae infection and rice suspension cells subjected to chitin treatment. Our analysis revealed the transcriptional regulation of well-known immune-related subfamilies of OsPRRs, OsRLCKs, and OsTFs, such as receptor-like kinase-leucine-rich repeat XII (RLK-LRR-XII) and RLCK-VII, and identified several novel subfamilies with high proportions of DEGs that may contribute to pathogen perception and plant defense. We demonstrated that selecting candidates from overlapping DEGs between plant and suspension cell systems is an effective strategy for screening genes involved in rice immunity. Using this pipeline, novel immune regulators were identified, and their functions were confirmed. Two RLCKs, i.e. OsRLCK298 and OsBSR1, act as positive regulators of immunity against rice blast fungus, whereas two TFs, i.e. OsERF65 and OsERF96.2, act as negative regulators. This study provides a valuable transcriptomic resource and establishes a validated pipeline for gene discovery that could be applied to other stress responses and in other plant species.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"976-1001"},"PeriodicalIF":4.7,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13317940/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146150524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AtCRCT1 and AtCRCT2, homologs of CO2 responsive CCT protein in Arabidopsis thaliana, are involved in the regulation of starch synthesis-related gene expression. AtCRCT1和AtCRCT2是拟南芥CO2 Responsive CCT Protein的同源基因，参与淀粉合成相关基因的表达调控。

IF 4.7 2区生物学

Plant and Cell Physiology Pub Date : 2026-06-30 DOI: 10.1093/pcp/pcag011

Hiroshi Fukayama, Ryosuke Toge, Nozumu Chuuma, Asuka Masaki, Hikaru Hagiwara, Fumihiro Miyagawa, Daisuke Sasayama, Tomoko Hatanaka, Tetsushi Azuma, Ryutaro Morita

{"title":"AtCRCT1 and AtCRCT2, homologs of CO2 responsive CCT protein in Arabidopsis thaliana, are involved in the regulation of starch synthesis-related gene expression.","authors":"Hiroshi Fukayama, Ryosuke Toge, Nozumu Chuuma, Asuka Masaki, Hikaru Hagiwara, Fumihiro Miyagawa, Daisuke Sasayama, Tomoko Hatanaka, Tetsushi Azuma, Ryutaro Morita","doi":"10.1093/pcp/pcag011","DOIUrl":"10.1093/pcp/pcag011","url":null,"abstract":"The CO2-responsive CCT protein (CRCT) is a transcription factor that regulates the expression of starch synthesis-related genes, and consequently starch content in the vegetative organs of rice. Two CRCT homologs, AtCRCT1 and AtCRCT2, were identified in the genome of Arabidopsis thaliana. AtCRCT1 and AtCRCT2 were expressed in most organs of the plant, and AtCRCT2 in particular was expressed in their vascular bundles. The expression of these genes was markedly upregulated by sucrose treatment and tended to increase under elevated CO2 conditions. The overexpression or knockout of these genes did not have a notable effect on the growth of Arabidopsis. On the other hand, the expression of some starch synthesis-related genes, such as the ADP-glucose pyrophosphorylase large subunit3 (AtAPL3) and the glucose 6-phosphate/Pi transporter2 (AtGPT2) was significantly upregulated in the overexpression lines. Accordingly, the expression of AtAPL3 and AtGPT2 in the double knockout line was significantly lower than that in the wild-type (Col-0) under sucrose treatment. In addition, the starch content of the double knockout line at the end of the night was slightly lower than that of Col-0. Yeast two-hybrid and bimolecular fluorescence complementation analyses demonstrated that both AtCRCT1 and AtCRCT2 interact with Growth Regulation Factor 7, a 14-3-3 protein. These results suggest that AtCRCT1 and AtCRCT2 have similar molecular and physiological functions to CRCT in rice and regulate the expression of some starch synthesis-related genes. However, their effects are limited and cannot markedly affect starch content, unlike CRCT in rice.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"910-916"},"PeriodicalIF":4.7,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146066491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A C-12-hydroxylating dioxygenase contributes to steroidal glycoalkaloid diversity in wild potato. C-12羟化双加氧酶对野生马铃薯甾体糖生物碱多样性的影响。

IF 4.7 2区生物学

Plant and Cell Physiology Pub Date : 2026-06-30 DOI: 10.1093/pcp/pcag022

Ryota Akiyama, Yuki Ikeyama, Hyoung Jae Lee, Bunta Watanabe, Naoyuki Umemoto, Kenji Asano, Toshiya Muranaka, Kazuki Saito, Yukihiro Sugimoto, Masaharu Mizutani

{"title":"A C-12-hydroxylating dioxygenase contributes to steroidal glycoalkaloid diversity in wild potato.","authors":"Ryota Akiyama, Yuki Ikeyama, Hyoung Jae Lee, Bunta Watanabe, Naoyuki Umemoto, Kenji Asano, Toshiya Muranaka, Kazuki Saito, Yukihiro Sugimoto, Masaharu Mizutani","doi":"10.1093/pcp/pcag022","DOIUrl":"10.1093/pcp/pcag022","url":null,"abstract":"Steroidal glycoalkaloids (SGAs), a class of specialized metabolites, are predominantly found in Solanum species. These compounds are recognized for their structural diversity and wide range of biological activities, including toxicity toward various plant pathogens and herbivores. The cultivated potato (Solanum tuberosum) primarily accumulates α-solanine and α-chaconine-bitter-tasting, toxic SGAs that together constitute >90% of the total SGAs. In contrast, the wild potato Solanum chacoense produces an array of rare SGAs, some reported to confer resistance to specific pests. However, the biosynthetic pathways of these uncommon SGAs remain poorly defined. In this study, we isolated and structurally characterized six SGAs from S. chacoense absent in cultivated potato, including two novel compounds: 12α-hydroxysolanine and 12α-hydroxychaconine. Recent evidence indicates that 2-oxoglutarate-dependent dioxygenases (DOXs) contribute to SGA structural diversification. Guided by this, we identified five candidate genes encoding DOX enzymes in S. chacoense. Biochemical assays revealed that one enzyme, designated Sc12DOX, catalyzes the C-12α hydroxylation of α-solanine and α-chaconine to yield 12α-hydroxysolanine and 12α-hydroxychaconine, respectively. These findings enhance our understanding of SGA structural and metabolic diversity within Solanum species.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"1013-1024"},"PeriodicalIF":4.7,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146182086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Volatile organic compounds prime heat tolerance through chromatin-dependent heat shock pathways in Arabidopsis. 挥发性有机化合物通过染色质依赖的热休克途径提高拟南芥的耐热性。

IF 4.7 2区生物学

Plant and Cell Physiology Pub Date : 2026-06-30 DOI: 10.1093/pcp/pcag028

Baibhav R Barbaruah, Feng Shuo, Hidetaka Ito

{"title":"Volatile organic compounds prime heat tolerance through chromatin-dependent heat shock pathways in Arabidopsis.","authors":"Baibhav R Barbaruah, Feng Shuo, Hidetaka Ito","doi":"10.1093/pcp/pcag028","DOIUrl":"10.1093/pcp/pcag028","url":null,"abstract":"Volatile organic compounds (VOCs) can prime plants for improved survival under heat stress, yet how chromatin regulation shapes VOC-triggered heat shock responses remains unclear. Here, we analyzed heat and VOC responses in Arabidopsis thaliana Col-0 and the epigenetic mutants ddm1, nrpd1, suvh456, and hda6. In a seven-VOC screen, heat and multiple VOCs induced HSFA2, with trans-2-hexenal (T2H) among the most consistent inducers. Although ddm1 and hda6 showed reduced HSFA2 induction under heat, VOC induction remained strong, indicating that volatile signaling can partially rebalance heat-dependent regulation. Priming with the VOC panel increased survival after lethal heat across genotypes, and phenotype scoring (Healthy/Affected/Dead) revealed genotype-dependent outcome shifts, including a stronger T2H-driven enrichment of Healthy seedlings in hda6. Expression profiling of HSFA1a-e regulators and the downstream chaperone HSP101 indicated intact pathway competence, while revealing quantitative rewiring in hda6. RNA-seq showed that T2H activates a program that is distinct from, but partially overlaps with, the heat response, combining a shared stress core with VOC-biased signaling and reactive oxygen species (ROS)-related modules that are amplified in hda6. Finally, analysis of public ChIP-seq datasets linked altered promoter enrichment of active marks (H3Ac and H3K4me2), particularly at HDA6-bound promoters, to basal transcript output and uneven tuning of VOC- and heat-/ROS-responsive loci. Together, these results support a model in which chromatin regulation tunes both the basal state and inducibility of VOC-responsive stress networks, thereby shaping VOC-primed thermotolerance.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"1037-1051"},"PeriodicalIF":4.7,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147318105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0