Plant and Cell Physiology最新文献

De-etiolation is Almost Colour Blind: the Study of Photosynthesis Awakening Under Blue and Red Light. 去叶绿素几乎是色盲的：蓝光和红光下光合作用觉醒的研究。

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2024-10-11 DOI: 10.1093/pcp/pcae119

Agnieszka K Banaś, Katarzyna Leja, Piotr Zgłobicki, Paweł Jedynak, Ewa Kowalska, Wojciech Strzałka, Joanna Grzyb, Beata Myśliwa-Kurdziel

{"title":"De-etiolation is Almost Colour Blind: the Study of Photosynthesis Awakening Under Blue and Red Light.","authors":"Agnieszka K Banaś, Katarzyna Leja, Piotr Zgłobicki, Paweł Jedynak, Ewa Kowalska, Wojciech Strzałka, Joanna Grzyb, Beata Myśliwa-Kurdziel","doi":"10.1093/pcp/pcae119","DOIUrl":"https://doi.org/10.1093/pcp/pcae119","url":null,"abstract":"The synthesis and assembly of functioning photosynthetic complexes in chloroplasts developing from etioplasts during the de-etiolation of angiosperm seedlings are imperative for the plant's autotrophic lifestyle. This study compared de-etiolation process under monochromatic red or blue light of equal photon flux density during a 24-hour illumination period of etiolated Arabidopsis seedlings. The aim was to elucidate the impact of these light wavelength on the etioplast-to-chloroplast transformation and the initiation of light-dependent photosynthetic reactions. Both treatments lead to the formation of functional young chloroplasts; however, the etioplast-to-chloroplast transition and the assembly of photosynthetic complexes occurred unevenly, with individual steps tuned by red or blue light. Ultrastructural analysis suggested faster prolamellar bodies disassembly under blue light, while low temperature fluorescence studies indicated a slower transformation of protochlorophyllide to chlorophyllide, and chlorophyll a, under these conditions. Red light further promoted the synthesis of chlorophyll b and LHCII antenna proteins. However, the efficiency of antennae in dissipating excess absorbed energy was higher for seedlings de-etiolated under blue light; the maximum quantum yield of the photosystem II reached 0.81 after 24-hour de-etiolation, equivalent to mature plants. Blue light seemed to enhance the development of well-functioning photosystems (I and II) and antennae. These findings are important for gaining a deeper understanding of photoreceptor regulation of de-etiolation and for utilizing selected light regimes to improve crop yield.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142406804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Convergent emergence of Glucomannan β-galactosyltransferase activity in Asterids and Rosids. 菊科植物和蔷薇科植物中葡萄糖甘露聚糖β-半乳糖基转移酶活性的趋同性。

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2024-10-11 DOI: 10.1093/pcp/pcae118

Konan Ishida, Matthew Penner, Kenji Fukushima, Yoshihisa Yoshimi, Louis F L Wilson, Alberto Echevarría-Poza, Li Yu, Paul Dupree

{"title":"Convergent emergence of Glucomannan β-galactosyltransferase activity in Asterids and Rosids.","authors":"Konan Ishida, Matthew Penner, Kenji Fukushima, Yoshihisa Yoshimi, Louis F L Wilson, Alberto Echevarría-Poza, Li Yu, Paul Dupree","doi":"10.1093/pcp/pcae118","DOIUrl":"https://doi.org/10.1093/pcp/pcae118","url":null,"abstract":"β-Galactoglucomannan (β-GGM) is a primary cell wall polysaccharide in rosids and asterids. The β-GGM polymer has a backbone of repeating β-(1,4)-glucosyl and mannosyl residues, usually with mono- α-(1,6)-galactosyl substitution or β-(1,2)-galactosyl α-galactosyl disaccharide sidechains on the mannosyl residues. Mannan β-GalactosylTransferases (MBGTs) are therefore required for β-GGM synthesis. The single MBGT identified so far, AtMBGT1, lies in glycosyltransferase family 47A subclade VII, and was identified in Arabidopsis. However, despite the presence of β-GGM, an orthologous gene is absent in tomato (Solanum lycopersicum), a model asterid. In this study, we screened candidate MBGT genes from the tomato genome, functionally tested the activities of encoded proteins, and identified the tomato MBGT (SlMBGT1) in GT47A-III. Interestingly therefore, AtMBGT1 and SlMBGT1 are located in different GT47A subclades. Further, phylogenetic and glucomannan structural analysis from different species raised the possibility that various asterids possess conserved MBGTs in an asterid-specific subclade of GT47A-III, indicating that MBGT activity has been acquired convergently among asterids and rosids. The present study highlights the promiscuous emergence of donor and acceptor preference in GT47A enzymes. The independent acquisition of the activity also suggests an adaptive advantage for eudicots to acquire β-GGM β-galactosylation, and hence also suggests the disaccharide side chains are important for β-GGM function.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142406803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gene targeting in Arabidopsis through one-armed homology-directed repair. 拟南芥通过单臂同源定向修复实现基因靶向。

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2024-10-05 DOI: 10.1093/pcp/pcae117

Michael Mudgett, Bradley Abramson, Xinhua Dai, Ruofan Kang, Ethan Young, Todd Michael, Yunde Zhao

引用次数: 0

The Armor of Orchid Petals: Insights into Cuticle Deposition Regulation. 兰花花瓣的盔甲：洞察角质层沉积调节

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2024-10-04 DOI: 10.1093/pcp/pcae116

Xiujuan Yang

引用次数: 0

Identification of High Linoleic Acid Varieties in Tetraploid perilla through Gamma-ray Irradiation and CRISPR/Cas9. 通过伽马射线照射和 CRISPR/Cas9 鉴定四倍体紫苏中的高亚油酸品种。

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2024-10-03 DOI: 10.1093/pcp/pcae084

Mid-Eum Park, Hyun-A Choi, Kyeong-Ryeol Lee, Jae Bok Heo, Hyun Uk Kim

{"title":"Identification of High Linoleic Acid Varieties in Tetraploid perilla through Gamma-ray Irradiation and CRISPR/Cas9.","authors":"Mid-Eum Park, Hyun-A Choi, Kyeong-Ryeol Lee, Jae Bok Heo, Hyun Uk Kim","doi":"10.1093/pcp/pcae084","DOIUrl":"10.1093/pcp/pcae084","url":null,"abstract":"Perilla [Perilla frutescens (L.) var frutescens] is a traditional oil crop in Asia, recognized for its seeds abundant in α-linolenic acid (18:3), a key omega-3 fatty acid known for its health benefits. Despite the known nutritional value, the reason behind the higher 18:3 content in tetraploid perilla seeds remained unexplored. Gamma irradiation yielded mutants with altered seed fatty acid composition. Among the mutants, DY-46-5 showed a 27% increase in 18:2 due to the 4-bp deletion of PfrFAD3b, and NC-65-12 displayed a 16% increase in 18:2 due to the loss of function of PfrFAD3a through a large deletion. Knocking out both copies of FATTY ACID DESATURASE3 (PfrFAD3a and PfrFAD3b) simultaneously using CRISPR/Cas9 resulted in an increase in 18:2 by up to 75% and a decrease in 18:3 to as low as 0.3% in seeds, emphasizing the pivotal roles of both genes in 18:3 synthesis in tetraploid perilla. Furthermore, diploid Perilla citriodora, the progenitor of cultivated tetraploid perilla, harbors only PfrFAD3b, with a fatty acid analysis revealing lower 18:3 levels than tetraploid perilla. In conclusion, the enhanced 18:3 content in cultivated tetraploid perilla seeds can be attributed to the acquisition of two FAD3 copies through hybridization with wild-type diploid perilla.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141875769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ancient Origin of Acetyltransferases Catalyzing O-acetylation of Plant Cell Wall Polysaccharides. 催化植物细胞壁多糖 O-乙酰化的乙酰转移酶的古老起源

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2024-10-03 DOI: 10.1093/pcp/pcae070

Ruiqin Zhong, Earle R Adams, Zheng-Hua Ye

{"title":"Ancient Origin of Acetyltransferases Catalyzing O-acetylation of Plant Cell Wall Polysaccharides.","authors":"Ruiqin Zhong, Earle R Adams, Zheng-Hua Ye","doi":"10.1093/pcp/pcae070","DOIUrl":"10.1093/pcp/pcae070","url":null,"abstract":"Members of the domain of unknown function 231/trichome birefringence-like (TBL) family have been shown to be O-acetyltransferases catalyzing the acetylation of plant cell wall polysaccharides, including pectins, mannan, xyloglucan and xylan. However, little is known about the origin and evolution of plant cell wall polysaccharide acetyltransferases. Here, we investigated the biochemical functions of TBL homologs from Klebsormidium nitens, a representative of an early divergent class of charophyte green algae that are considered to be the closest living relatives of land plants, and Marchantia polymorpha, a liverwort that is an extant representative of an ancient lineage of land plants. The genomes of K. nitens and Marchantia polymorpha harbor two and six TBL homologs, respectively. Biochemical characterization of their recombinant proteins expressed in human embryonic kidney 293 cells demonstrated that the two K. nitens TBLs exhibited acetyltransferase activities acetylating the pectin homogalacturonan (HG) and hence were named KnPOAT1 and KnPOAT2. Among the six M. polymorpha TBLs, five (MpPOAT1 to 5) possessed acetyltransferase activities toward pectins and the remaining one (MpMOAT1) catalyzed 2-O- and 3-O-acetylation of mannan. While MpPOAT1,2 specifically acetylated HG, MpPOAT3,4,5 could acetylate both HG and rhamnogalacturonan-I. Consistent with the acetyltransferase activities of these TBLs, pectins isolated from K. nitens and both pectins and mannan from M. polymorpha were shown to be acetylated. These findings indicate that the TBL genes were recruited as cell wall polysaccharide O-acetyltransferases as early as in charophyte green algae with activities toward pectins and they underwent expansion and functional diversification to acetylate various cell wall polysaccharides during evolution of land plants.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141446882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogenetic Profiling Analysis of the Phycobilisome Revealed a Novel State-Transition Regulator Gene in Synechocystis sp. PCC 6803. 藻体系统发育分析揭示了 Synechocystis sp.

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2024-10-03 DOI: 10.1093/pcp/pcae083

Tsukasa Fukunaga, Takako Ogawa, Wataru Iwasaki, Kintake Sonoike

{"title":"Phylogenetic Profiling Analysis of the Phycobilisome Revealed a Novel State-Transition Regulator Gene in Synechocystis sp. PCC 6803.","authors":"Tsukasa Fukunaga, Takako Ogawa, Wataru Iwasaki, Kintake Sonoike","doi":"10.1093/pcp/pcae083","DOIUrl":"10.1093/pcp/pcae083","url":null,"abstract":"Phycobilisomes play a crucial role in the light-harvesting mechanisms of cyanobacteria, red algae and glaucophytes, but the molecular mechanism of their regulation is largely unknown. In the cyanobacterium, Synechocystis sp. PCC 6803, we identified slr0244 as a phycobilisome-related gene using phylogenetic profiling analysis, a method used to predict gene function based on comparative genomics. To investigate the physiological function of the slr0244 gene, we characterized slr0244 mutants spectroscopically. Disruption of the slr0244 gene impaired state transition, a process by which the distribution of light energy absorbed by the phycobilisomes between two photosystems is regulated in response to the changes in light conditions. The Slr0244 protein seems to act in the process of state transition, somewhere at or downstream of the sensing step of the redox state of the plastoquinone (PQ) pool. These findings, together with past reports describing the interaction of this gene product with thioredoxin and glutaredoxin, suggest that the slr0244 gene is a novel state-transition regulator that integrates the redox signal of PQ pools with that of the photosystem I-reducing side. The protein has two universal stress protein (USP) motifs in tandem. The second motif has two conserved cysteine residues found in USPs of other cyanobacteria and land plants. These redox-type USPs with conserved cysteines may function as redox regulators in various photosynthetic organisms. Our study also shows the efficacy of phylogenetic profiling analysis in predicting the function of cyanobacterial genes that have not been annotated so far.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11447641/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141734937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Light Color Regulation of Photosynthetic Antennae Biogenesis in Marine Phytoplankton. 海洋浮游植物光合触角生物发生的光色调节

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2024-10-03 DOI: 10.1093/pcp/pcae115

David M Kehoe, Avijit Biswas, Bo Chen, Louison Dufour, Théophile Grébert, Allissa M Haney, Kes Lynn Joseph, Indika Kumarapperuma, Adam A Nguyen, Morgane Ratin, Joseph E Sanfilippo, Animesh Shukla, Laurence Garczarek, Xiaojing Yang, Wendy M Schluchter, Frédéric Partensky

{"title":"Light Color Regulation of Photosynthetic Antennae Biogenesis in Marine Phytoplankton.","authors":"David M Kehoe, Avijit Biswas, Bo Chen, Louison Dufour, Théophile Grébert, Allissa M Haney, Kes Lynn Joseph, Indika Kumarapperuma, Adam A Nguyen, Morgane Ratin, Joseph E Sanfilippo, Animesh Shukla, Laurence Garczarek, Xiaojing Yang, Wendy M Schluchter, Frédéric Partensky","doi":"10.1093/pcp/pcae115","DOIUrl":"https://doi.org/10.1093/pcp/pcae115","url":null,"abstract":"Photosynthesis in the world's oceans is primarily conducted by phytoplankton, microorganisms that use many different pigments for light capture. Synechococcus is a unicellular cyanobacterium estimated to be the second most abundant marine phototroph, with a global population of 7 x 1026 cells. This group's success is partly due to the pigment diversity in their photosynthetic light harvesting antennae, which maximize photon capture for photosynthesis. Many Synechococcus isolates adjust their antennae composition in response to shifts in the blue:green ratio of ambient light. This response was named Type 4 chromatic acclimation (CA4). Research has made significant progress in understanding CA4 across scales, from its global ecological importance to its molecular mechanisms. Two forms of CA4 exist, each correlated with the occurrence of one of two distinct but related genomic islands. Several genes in these islands are differentially transcribed by the ambient blue:green light ratio. The encoded proteins control the addition of different pigments to the antennae proteins in blue versus green light, altering their absorption characteristics to maximize photon capture. These genes are regulated by several putative transcription factors also encoded in the genomic islands. Ecologically, CA4 is the most abundant of marine Synechococcus pigment types, occurring in over 40% of the population oceanwide. It predominates at higher latitudes and at depth, suggesting that CA4 is most beneficial under sub-saturating photosynthetic light irradiances. Future CA4 research will further clarify the ecological role of CA4 and the molecular mechanisms controlling this globally important form of phenotypic plasticity.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142366327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PCP Research Highlights-Plant Systemic Signaling: Bridging Distances with Mobile Molecules. PCP 研究亮点 - 植物系统信号：用移动分子拉近距离。

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2024-10-03 DOI: 10.1093/pcp/pcae075

Hiroki Tsutsui

引用次数: 0

Radicle Growth Regulation of Root Parasitic Plants by Auxin-related Compounds. 辅助素相关化合物对根寄生植物胚根生长的调节作用

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2024-10-03 DOI: 10.1093/pcp/pcae071

Kei Tsuzuki, Taiki Suzuki, Michio Kuruma, Kotaro Nishiyama, Ken-Ichiro Hayashi, Shinya Hagihara, Yoshiya Seto

{"title":"Radicle Growth Regulation of Root Parasitic Plants by Auxin-related Compounds.","authors":"Kei Tsuzuki, Taiki Suzuki, Michio Kuruma, Kotaro Nishiyama, Ken-Ichiro Hayashi, Shinya Hagihara, Yoshiya Seto","doi":"10.1093/pcp/pcae071","DOIUrl":"10.1093/pcp/pcae071","url":null,"abstract":"Root parasitic plants in the Orobanchaceae, such as Striga and Orobanche, cause significant damage to crop production. The germination step of these root parasitic plants is induced by host-root-derived strigolactones. After germination, the radicles elongate toward the host and invade the host root. We have previously discovered that a simple amino acid, tryptophan (Trp), as well as its metabolite, the plant hormone indole-3-acetic acid (IAA), can inhibit radicle elongation of Orobanche minor. These results suggest that auxin plays a crucial role in the radicle elongation step in root parasitic plants. In this report, we used various auxin chemical probes to dissect the auxin function in the radicle growth of O. minor and Striga hermonthica. We found that synthetic auxins inhibited radicle elongation. In addition, auxin receptor antagonist, auxinole, rescued the inhibition of radicle growth by exogenous IAA. Moreover, a polar transport inhibitor of auxin, N-1-naphthylphthalamic acid, affected radicle bending. We also proved that exogenously applied Trp is converted into IAA in O. minor seeds, and auxinole partly rescued this radicle elongation. Taken together, our data demonstrate a pivotal role for auxin in radicle growth. Thus, manipulation of auxin function in root parasitic plants should offer a useful approach to combat these parasites.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141470383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0