Plant and Cell Physiology最新文献_第2页

Development of a Rapid Transformation and Amplification System in Byblis guehoi Unveils an Atypical Protein Secretion Pathway in Sessile Trichome Cells. 一种快速转化扩增系统的建立揭示了毛状细胞非典型蛋白分泌途径。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2026-04-20 DOI: 10.1093/pcp/pcag050

You-Xian Li, Chia-Hua Yu, I-Jou Hsu, Yen-Zhen Chen, Yu-Wen Huang, Alvin Chen, Wei-Ming Leu

{"title":"Development of a Rapid Transformation and Amplification System in Byblis guehoi Unveils an Atypical Protein Secretion Pathway in Sessile Trichome Cells.","authors":"You-Xian Li, Chia-Hua Yu, I-Jou Hsu, Yen-Zhen Chen, Yu-Wen Huang, Alvin Chen, Wei-Ming Leu","doi":"10.1093/pcp/pcag050","DOIUrl":"10.1093/pcp/pcag050","url":null,"abstract":"The leaves of the carnivorous plant Byblis guehoi (B. guehoi) are densely covered with structurally simple sessile trichomes, each composed of eight flattened cells arranged in a ring. These trichomes exhibit robust protein exocytosis and endocytosis, making them a suitable system for studying these processes. To track protein secretion, we established an Agrobacterium-mediated transformation protocol using root explants. Calli were induced on Byblis Callus Induction Medium (BCIM), and transgenic shoots were regenerated within two months on hygromycin-containing Byblis Selection and Regeneration Medium (BSRM). In addition, a temporary immersion system (TIS) was implemented to rapidly propagate transgenic lines, enhancing growth and facilitating acclimation. Four 35S promoter-driven constructs expressing GUS, RUBY, mGFP5, or CLV3sp-mGFP5 validated the protocol, yielding transformation efficiencies of 24.9-59.4% and regeneration efficiencies of 22.3-73.7%. Confocal microscopy revealed predominantly cytoplasmic signals for mGFP5 and plasma membrane-localized signals for CLV3sp-mGFP5 in epidermal cells. Unexpectedly, both constructs exhibited GFP signals at the plasma membrane and in the extracellular space of sessile trichome cells, regardless of the presence of a signal peptide. Western blot analysis further confirmed that mGFP5 was secreted into the mucilage independently of signal peptide presence. These findings reveal signal peptide-independent extracellular protein accumulation in glandular trichomes, deviating from the classical ER-Golgi pathway. Given that sessile trichomes of B. guehoi possess a specialized cuticular pore lacking a rigid cell wall, this structural feature may provide a plausible interface for non-classical protein export. This study establishes a rapid transformation-propagation pipeline for B. guehoi, highlighting its potential as an experimental platform for investigating protein secretion in plant glandular cells.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147723509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functions and interactions of important sulfur-containing metabolites in plant development and stress responses. 重要含硫代谢物在植物发育和胁迫反应中的作用和相互作用。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2026-04-15 DOI: 10.1093/pcp/pcag045

Zhigang Wang, Fan Chen, Yunfei Xu, Yijie Zang, Wenjia Chen, Yunxiang Zang, Huihui Fang

{"title":"Functions and interactions of important sulfur-containing metabolites in plant development and stress responses.","authors":"Zhigang Wang, Fan Chen, Yunfei Xu, Yijie Zang, Wenjia Chen, Yunxiang Zang, Huihui Fang","doi":"10.1093/pcp/pcag045","DOIUrl":"https://doi.org/10.1093/pcp/pcag045","url":null,"abstract":"Sulfur (S), an essential macroelement, plays important roles in plant numerous physiological processes. Through sulfur assimilation pathways, plants biosynthesize various S-containing compounds, including the gasotransimitter hydrogen sulfide (H2S), the primary organic S-containing amino acid cysteine (Cys), the master antioxidant glutathione (GSH), and the characteristic secondary metabolites such as glucosinolates (GSLs). Current studies have established the wide involvement of S-containing metabolites in orchestrating plant development and stimuli responses, however, the interplay and crosstalk among these molecules in coordinating these processes remain limited. This review systematically synthesizes current knowledge on sulfur assimilation and the multifaceted functions of S-containing metabolites in plants, encompassing their roles in ROS and intracellular sulfur homeostasis, protein redox modifications, and their function as signaling molecules that interact with plant hormones and other growth regulators. We also specifically delineate the functional interactions and crosstalk among some metabolites, with a focus on the paired relationships between H2S and Cys, H2S and GSH, H2S and GSLs, Cys and GSLs, and GSH and GSLs, during plant development and stress adaptation. We expect that this review will establish a comprehensive framework for understanding the regulation of sulfur metabolism and elucidate the mechanistic basis of S-containing metabolites in physiological processes. By integrating recent advances in this field, we aim to provide not only fundamental insights into sulfur nutrient utilization efficiency, but also valuable theoretical support for developing innovative strategies in crop improvement and sustainable agriculture.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147691803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Redox potentials of carotenoids associated with type-II photosynthetic reaction centers. 类胡萝卜素与ii型光合反应中心相关的氧化还原电位。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2026-04-14 DOI: 10.1093/pcp/pcaf167

Keisuke Saito, Hiroshi Ishikita

{"title":"Redox potentials of carotenoids associated with type-II photosynthetic reaction centers.","authors":"Keisuke Saito, Hiroshi Ishikita","doi":"10.1093/pcp/pcaf167","DOIUrl":"10.1093/pcp/pcaf167","url":null,"abstract":"Carotenoids play dual roles in photosynthesis, extending light absorption and providing photoprotection through radical cation formation. Their ability to act as electron donors is defined by their redox potential for one-electron oxidation (Em). Here, we determine Em for carotenoids whose Em values are missing or inconsistently established in type-II reaction centers or associated antenna complexes, using a quantum chemical approach. The highest occupied molecular orbital (HOMO) energy levels correlated strongly with reported Em values in CH2Cl2, enabling the determination of reliable Em values. All-trans isomers were found to be slightly stronger electron donors than their 15-cis counterparts. As this Em difference is small, the biological significance of cis isomerization appears to lie in pigment orientation rather than intrinsic redox properties. For xanthophyll-cycle pigments, a stepwise increase in Em from zeaxanthin to antheraxanthin to violaxanthin, attributable to their substituents, was revealed. Em values measured in micelles deviated markedly not only from those in CH2Cl2 but also from HOMO energy levels in water, questioning their relevance as a reference value in protein environments. These results provide a unified framework for understanding carotenoid redox chemistry in photosynthesis.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"367-376"},"PeriodicalIF":4.0,"publicationDate":"2026-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13078154/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145744085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship between open reading frame 320, a gene causing male sterility in tomatoes, and cytoplasmic male sterility in potatoes. 番茄雄性不育基因320与马铃薯细胞质雄性不育的关系

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2026-04-14 DOI: 10.1093/pcp/pcaf157

Rika Nakajima, Rena Sanetomo, Kenta Shirasawa, Tohru Ariizumi, Kosuke Kuwabara

{"title":"Relationship between open reading frame 320, a gene causing male sterility in tomatoes, and cytoplasmic male sterility in potatoes.","authors":"Rika Nakajima, Rena Sanetomo, Kenta Shirasawa, Tohru Ariizumi, Kosuke Kuwabara","doi":"10.1093/pcp/pcaf157","DOIUrl":"10.1093/pcp/pcaf157","url":null,"abstract":"Cytoplasmic male sterility (CMS) is a trait wherein plants cannot develop normal male organs because of mitochondrial genes. In potatoes (Solanum tuberosum), reports on the relevant mitochondrial genes remain scarce. Many potato cultivars express pollen sterility caused by mitochondria, thereby limiting their use as male parents in breeding. Therefore, identifying the causal genes is crucial for potato breeding. In this study, we focused on the T/β cytoplasm type, the most prevalent cytoplasm of potato worldwide, to explore mitochondrial genes involved in CMS in potatoes. We identified a novel gene, open reading frame 320 (orf320) from potato with T/β type cytoplasm by comparing the mitochondrial genomes. The accumulation level of orf320 transcripts was drastically reduced in the anthers of a fertile potato cultivar compared with those of a sterile cultivar. Functional analysis of tomatoes showed that overexpression of orf320 with a mitochondrial transit peptide induced male sterility phenotype accompanying abnormal anther development and pollen abortion. Furthermore, an investigation of orf320 in 124 potato cultivars revealed that this gene is tightly associated with the T/β type cytoplasm and is absent from cultivars with other cytoplasm types. These findings provide evidence that orf320 is a candidate CMS-causing gene in male sterility of T/β type cytoplasm, offering valuable insights for future potato breeding.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"289-300"},"PeriodicalIF":4.0,"publicationDate":"2026-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145605164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gain, loss, and fusion: ancient and eventful origin of DIVARICATA and DRIF genes. 获得，失去和融合：DIVARICATA和DRIF基因的古老而重要的起源。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2026-04-14 DOI: 10.1093/pcp/pcaf145

Aniket Sengupta, Dianella Howarth

{"title":"Gain, loss, and fusion: ancient and eventful origin of DIVARICATA and DRIF genes.","authors":"Aniket Sengupta, Dianella Howarth","doi":"10.1093/pcp/pcaf145","DOIUrl":"10.1093/pcp/pcaf145","url":null,"abstract":"Comprising one of the largest plant gene families, MYB genes are major regulators of growth and development across plant tissues. Their evolutionary history is complex with recurrent gain and loss of the MYB domains, sometimes within the same multi-domained gene, creating a reticulate phylogenetic history, with various parts of the same gene having conflicting phylogenetic histories. Multiple MYB genes may co-operate or compete, thus constituting an on/off switch regulating transcription of downstream genes. We determined the phylogenetic origin of the multi-domained MYB regulators called DIVARICATA (DIV) genes, their cofactors the DRIF genes, and their competitors the LFG genes. We report that DIV arose through the fusion of two simpler MYB genes that resulted in a gene with three MYB domains (MYBA-MYB1-MYB2). The MYBA domain was later lost through a nongradual process resulting in the two-domained MYB1-MYB2 DIV genes in green plants (including flowering plants). Further truncation of the MYB1 domain resulted in LFG genes that have only the MYB2 domain. The MYBA and the MYB2 domains were derived from the SHAQKY clade of MYB domains; the MYB1 domain and the MYBD domain of DRIF were derived from the clade associated with the SANT2 domain of ZUO1/ZRF genes. We discuss how the duplication and truncation of DIV has been repeatedly recruited in the evolution of on/off switches. Components of the DIV-based regulatory network, and their close homologs, are present in a diversity of eukaryotes suggesting that their interaction may be ancestral to a large group of eukaryotes.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"250-264"},"PeriodicalIF":4.0,"publicationDate":"2026-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145565020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rab geranylgeranyl transferase activity is required for proper sterol biosynthesis in Arabidopsis thaliana. 拟南芥中适宜的甾醇生物合成需要香叶转移酶活性。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2026-04-14 DOI: 10.1093/pcp/pcaf166

Małgorzata Gutkowska, Marta Zajbt-Łuczniewska, Daniel Buszewicz, Anna Anielska-Mazur, Agata Lipko, Cezary Pączkowski, Grzegorz Spólnik, Maciej Sojka, Radosław Jaźwiec, Emilia Samborowska, Ewa Swiezewska, Marta Hoffman

{"title":"Rab geranylgeranyl transferase activity is required for proper sterol biosynthesis in Arabidopsis thaliana.","authors":"Małgorzata Gutkowska, Marta Zajbt-Łuczniewska, Daniel Buszewicz, Anna Anielska-Mazur, Agata Lipko, Cezary Pączkowski, Grzegorz Spólnik, Maciej Sojka, Radosław Jaźwiec, Emilia Samborowska, Ewa Swiezewska, Marta Hoffman","doi":"10.1093/pcp/pcaf166","DOIUrl":"10.1093/pcp/pcaf166","url":null,"abstract":"In all eukaryotic cells, protein prenylation and cytoplasmic isoprenoid biosynthesis pathways leading to sterols, dolichols, and other isoprenoid compounds share a common precursor pool of isopentenyl diphosphate and the isomeric dimethylallyl diphosphate. Despite this, little is known about the interplay between these processes. Here we ask whether perturbation of protein prenylation in plants influences isoprenoid biosynthesis in the endoplasmic reticulum, and in particular if it affects sterol and dolichol biosynthesis. We use an Arabidopsis thaliana mutant with defects in the Rab geranylgeranyl transferase as a viable model of protein hypoprenylation, and we show that sterol and dolichol content is significantly elevated in the mutant plants. Also sterol composition is changed: cholesterol content is increased and some atypical sterol pathway intermediates are accumulating. Our results show that plant sterol biosynthesis involves high levels of crosstalk between pathway branches than previously reported and receives regulatory input from protein prenylation pathways.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"346-366"},"PeriodicalIF":4.0,"publicationDate":"2026-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13078166/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145715428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant UBX domain-containing proteins use distinct strategies to stably engage the unfoldase CDC48A. 植物UBX结构域蛋白使用不同的策略稳定地参与CDC48A展开酶。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2026-04-14 DOI: 10.1093/pcp/pcaf173

Junrui Zhang, Jun Wang, Anandsukeerthi Sandholu, Umar F Shahul Hameed, Stefan T Arold

{"title":"Plant UBX domain-containing proteins use distinct strategies to stably engage the unfoldase CDC48A.","authors":"Junrui Zhang, Jun Wang, Anandsukeerthi Sandholu, Umar F Shahul Hameed, Stefan T Arold","doi":"10.1093/pcp/pcaf173","DOIUrl":"10.1093/pcp/pcaf173","url":null,"abstract":"The AAA+ ATPase CDC48A is a central regulator of proteostasis in plants, functioning through interactions with a diverse set of cofactors. Among these, the plant-specific ubiquitin regulatory X (UBX) domain-containing proteins (PUX) are key adaptors that direct CDC48A to specific substrates and pathways. The molecular basis of PUX-CDC48A interactions remains incompletely understood. Here, we combined structural, biophysical, and computational approaches to dissect the binding modes of representative PUX proteins from different subfamilies in Arabidopsis thaliana. Although all PUX proteins tested exhibit low micromolar affinities for CDC48A, they form unexpectedly stable complexes, suggesting additional mechanisms of interaction. We identified two distinct strategies for complex stabilization, producing different dynamic features. One relies on combining two weak associations: PUX5 employs a SHP-UBX module that engages the CDC48A N domain at two proximal sites, whereas PUX2 uses a SHP motif and a distant PUB domain to engage the N- and C-termini of CDC48A. In contrast, PUX6, PUX7, and PUX9 allosterically stabilize the association between their UBX domain and the CDC48A N domain. These multi-pronged strategies likely enable durable, yet reversible associations, facilitating fine-tuned competitive regulation of CDC48A activity across diverse cellular contexts. Our findings provide a mechanistic framework for understanding how PUX proteins achieve specificity, stability, and regulatory flexibility in directing CDC48A function.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"236-249"},"PeriodicalIF":4.0,"publicationDate":"2026-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13078151/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145850693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Calmodulin-dependent protein kinase-related kinase CRK5 contributes to the regulation of the PIN3 cellular localization domain upon plant weight increase. CRK5参与体重增加时PIN3细胞定位域的调控。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2026-04-14 DOI: 10.1093/pcp/pcaf168

Paula Brunot-Garau, Àngela Carrió-Seguí, Javier Agustí

引用次数: 0

Xylan reducing end sequence-containing oligosaccharides function as priming acceptors for Arabidopsis and Setaria viridis IRX10. 含木聚糖还原末端序列的低聚糖作为拟南芥和狗尾草IRX10的启动受体。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2026-04-14 DOI: 10.1093/pcp/pcaf154

Seichi Suzuki, Mayu Sakamoto, Haruki Toda, Yasuhiko Kizuka, Tadashi Ishii, Takeshi Ishimizu, Shiro Suzuki

{"title":"Xylan reducing end sequence-containing oligosaccharides function as priming acceptors for Arabidopsis and Setaria viridis IRX10.","authors":"Seichi Suzuki, Mayu Sakamoto, Haruki Toda, Yasuhiko Kizuka, Tadashi Ishii, Takeshi Ishimizu, Shiro Suzuki","doi":"10.1093/pcp/pcaf154","DOIUrl":"10.1093/pcp/pcaf154","url":null,"abstract":"Xylan, one of the most abundant hemicelluloses in plant cell walls, consists of β-(1 → 4)-linked xylosyl (Xyl) residues and often contains a conserved reducing end sequence (RES) in dicots and gymnosperms, comprising β-d-Xylp-(1 → 3)-α-l-Rhap-(1 → 2)-α-d-GalpA-(1 → 4)-d-Xylp. This tetrasaccharide has been proposed to function as a priming module (\"primer hypothesis\") or a termination signal (\"terminator hypothesis\") in xylan biosynthesis, yet its precise biochemical role remains unclear. Here, we examined whether the RES-containing oligosaccharide with one additional Xyl residue at the non-reducing end (X-RES) acts as a priming acceptor for IRREGULAR XYLEM10 (IRX10) proteins from a dicot, Arabidopsis thaliana, and a grass, Setaria viridis. Both recombinant AtIRX10L and SvIRX10 utilized fluorescently labeled X-RES and the canonical primer Xyl5 as acceptor substrates. Time-course analyses revealed that X-RES promoted a more efficient transition of +X1 to +X2 product, i.e. with minimal accumulation of the +X1 product and enhanced formation of the +X2 and longer products, suggesting that the RES motif facilitates seamless elongation. Consistent with these substrate-dependent differences, docking simulations showed that X-RES and its elongated form (X2-RES) bound more stably to the predicted IRX10 active site than the corresponding linear oligosaccharides Xyl5 and Xyl6. Moreover, the ability of SvIRX10 to recognize X-RES, despite the RES motif not yet being detected in grass xylan, suggests that the RES-primed elongation may represent an ancestral substrate recognition in grasses. Our findings identify a structurally unique RES-containing oligosaccharide that functions as a primer in vitro, thereby extending current understanding of acceptor substrate flexibility in xylan biosynthesis.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"275-288"},"PeriodicalIF":4.0,"publicationDate":"2026-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145588386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Conserved Sequence Insert of Fibrillins is Involved in Plastoglobule Association and Lipid Binding. 纤维蛋白的一个保守序列插入物参与了微质体结合和脂质结合。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2026-04-14 DOI: 10.1093/pcp/pcag047

Kiran-Kumar Shivaiah, Roselane Kithan-Lundquist, April K Kaneshiro, Duncan M Boren, Febri A Susanto, Andres Herrera-Tequia, Josh V Vermaas, Peter Knut Lundquist

{"title":"A Conserved Sequence Insert of Fibrillins is Involved in Plastoglobule Association and Lipid Binding.","authors":"Kiran-Kumar Shivaiah, Roselane Kithan-Lundquist, April K Kaneshiro, Duncan M Boren, Febri A Susanto, Andres Herrera-Tequia, Josh V Vermaas, Peter Knut Lundquist","doi":"10.1093/pcp/pcag047","DOIUrl":"https://doi.org/10.1093/pcp/pcag047","url":null,"abstract":"Plastoglobule lipid droplets of chloroplasts serve complex roles affecting plant development, stress tolerance and photosynthesis. They harbor a set of approximately 40 proteins that collectively dictate plastoglobule functions. Due to the monolayer structure of plastoglobules, which encompass a neutral lipid core, these proteins must associate monotopically on the plastoglobule surface. However, targeting determinants have not been identified for plastoglobule proteins, and the protein-membrane interaction mechanisms that establish the plastoglobule proteome remain unclear. Here, we demonstrate that most plastoglobule-localized Fibrillins harbor an extra sequence insert with predicted amphipathic helical structure, placing it at the lip of the β-barrel. Deletions of the insert in two plastoglobule-targeted FBNs disrupt their localization, indicating that they are necessary for effective plastoglobule association. Molecular dynamics simulations support the specific interaction of the amphipathic helical insert of AtFBN1a with membranes rich in lipid packing defects which are expected to be especially prevalent on the tightly curved surface of plastoglobules. Proteomic analyses indicate AtFBN1a influences the plastoglobule proteome through outcompeting and recruiting specific proteins. We also demonstrate that the plastoglobule-localized FBNs, AtFBN1a and AtFBN7a, bind unsaturated fatty acids, particularly C18:1, and that elimination of the amphipathic helical inserts suppress fatty acid binding in AtFBN1a, but promotes fatty acid binding in AtFBN7a. Predicted amphipathic helices can be identified on two-thirds of plastoglobule proteins, indicating the use of amphipathic helices may be a general mechanism by which proteins selectively associate with plastoglobules.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147691826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0