Plant and Cell Physiology最新文献_第2页

SpMS1, a male sterility factor, interacts with SpAP1 to regulate unisexual flower development in dioecious spinach. 雄性不育因子 SpMS1 与 SpAP1 相互作用，调控雌雄异株菠菜单性花的发育。

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-01-29 DOI: 10.1093/pcp/pcae135

Ning Li, Bingxin Wang, Xinran Shang, Qiangwei Yang, Liang Yang, Minjie Tao, Shoaib Muhammad, Ainong Shi, Chuanliang Deng

{"title":"SpMS1, a male sterility factor, interacts with SpAP1 to regulate unisexual flower development in dioecious spinach.","authors":"Ning Li, Bingxin Wang, Xinran Shang, Qiangwei Yang, Liang Yang, Minjie Tao, Shoaib Muhammad, Ainong Shi, Chuanliang Deng","doi":"10.1093/pcp/pcae135","DOIUrl":"10.1093/pcp/pcae135","url":null,"abstract":"The emergence of unisexual flower is an important event during plant evolution. The molecular mechanism underlying the formation of unisexual flowers remains unclear in dioecious spinach. In this study, we identified the spinach MALE STERILITY1 gene, SpMS1, which serves as a masculine factor to regulate male fertility and sex reversion. Silencing SpMS1 led to stamen sterility in male flowers and the development of masculine traits in female flowers. Overexpression of SpMS1 in wild-type Arabidopsis resulted in sterile stamens and irregular pollen exine. Notably, ectopic expression of SpMS1 in Arabidopsis ms1 mutants restored pollen viability and flower fertility. Furthermore, our findings demonstrate that SpMS1 interacts with MADS-box transcription factor SpAP1 to regulate unisexual flower development. Thus, SpMS1 exhibits a conserved function in pollen fertility akin to bisexual flowers, while also acting as a key regulator of unisexual flower development in spinach. This study sheds light on the mechanism of sex differentiation in dioecious plants and also provides valuable insights for manipulating male sterility in plant breeding programs.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"60-74"},"PeriodicalIF":3.9,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142688664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evidence for decoupled water potential between myco- and photobionts during desiccation in the tripartite lichen Peltigera britannica. 在三方地衣 Peltigera britannica 的干燥过程中，证明霉菌和光生菌体之间的水势脱钩。

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-01-29 DOI: 10.1093/pcp/pcae143

Francesco Petruzzellis, Enrico Boccato, Carlotta Musso, Teresa Craighero, Alice Montagner, Tadeja Savi, Lucia Muggia, Tor Tønsberg, Mauro Tretiach, Andrea Nardini, Fabio Candotto Carniel

{"title":"Evidence for decoupled water potential between myco- and photobionts during desiccation in the tripartite lichen Peltigera britannica.","authors":"Francesco Petruzzellis, Enrico Boccato, Carlotta Musso, Teresa Craighero, Alice Montagner, Tadeja Savi, Lucia Muggia, Tor Tønsberg, Mauro Tretiach, Andrea Nardini, Fabio Candotto Carniel","doi":"10.1093/pcp/pcae143","DOIUrl":"10.1093/pcp/pcae143","url":null,"abstract":"In lichens, accurate description of thallus water status is required to understand growth and photosynthesis dynamics. A recent model suggested that myco- and photobiont layers could have a different water energy status (i.e. a different water potential, Ψ) during thallus desiccation, but data supporting this hypothesis were presented only for one chlorolichen. We compared water relations and maximum photosystem II (PSII) efficiency during desiccation in the tripartite lichen Peltigera britannica and its axenically cultured photobionts, the green alga Coccomyxa sp. and the cyanobacterium Nostoc sp. To assess whether myco- and photobiont layers had different Ψ values during desiccation, we expected that (i) water relation parameters differ between cultured photobionts and entire thalli and (ii) Ψ values inducing a significant drop in PSII efficiency are lower for entire thalli than for cultured photobionts. We found that photobionts had very different water relation parameters than entire thalli, i.e. lower water potential at the turgor loss point and higher cell wall elasticity, irrespective of the photobiont type, potentially underlying a different drought tolerance. PSII efficiency in entire thalli and cultured photobionts started to decrease below Ψ values, inducing turgor loss. Importantly, PSII efficiency in entire thalli decreased at Ψ values significantly more negative than those inducing turgor loss in cultured photobionts. These data support the hypothesis of decoupled Ψ between myco- and photobionts in lichens during desiccation. A higher Ψ ensured to the photobiont layer might represent a key adaptation to prolong photosynthesis during desiccation.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"89-100"},"PeriodicalIF":3.9,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142829764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integration of DNA Methylation, MicroRNAome, Degradome and Transcriptome Provides Insights into Petunia Anther Development. 整合 DNA 甲基化、MicroRNA 组、降解组和转录组有助于深入了解矮牵牛花药的发育。

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-01-29 DOI: 10.1093/pcp/pcae126

Yuanzheng Yue, Wuwei Zhu, Jiahui Wang, Tengteng Wang, Lisha Shi, Hannah Rae Thomas, Huirong Hu, Lianggui Wang

{"title":"Integration of DNA Methylation, MicroRNAome, Degradome and Transcriptome Provides Insights into Petunia Anther Development.","authors":"Yuanzheng Yue, Wuwei Zhu, Jiahui Wang, Tengteng Wang, Lisha Shi, Hannah Rae Thomas, Huirong Hu, Lianggui Wang","doi":"10.1093/pcp/pcae126","DOIUrl":"10.1093/pcp/pcae126","url":null,"abstract":"Petunia hybrida is an annual herb flower that is prevalently cultivated both in public landscaping and home gardening. Anthers are vital reproductive organs for plants, but the molecular mechanism controlling petunia anther development remains elusive. In this work, we combined DNA methylation, microRNAome, degradome and transcriptome data to generate a comprehensive resource focused on exploring the complex molecular mechanism of petunia anther development. This study shows that DNA methylation could have an important impact in repressing the anther-expressed genes in the late stages of anther maturation. A total of 8,096 anther-preferential genes and 149 microRNAs (miRNAs) were identified that highly expressed in the five typical petunia anther developmental stages. Gene Ontology enrichment analysis of differentially expressed genes as well as miRNAs target genes revealed that metabolic, cellular and single-organism processes were significantly activated during the anther maturation processes. Moreover, a co-expression regulatory network for five typical anther development stages was constructed based on transcriptomic data, in which two hub transcription factors, PhERF48 and PhMS1, were demonstrated to be important regulatory genes for male fertility. Furthermore, two DNA demethylase proteins (PhDME and PhDML3) and three methyl-CpG-binding-domain proteins (PhMBD2, PhMBD3 and PhMBD4) were identified as potential critical DNA methylation regulators in petunia anther development. Our results provide new knowledge regarding the regulatory mechanism of petunia anther development, which will support the breeding of novel sterile petunia lines in the future.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"36-49"},"PeriodicalIF":3.9,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discovery of 4-(2-phenylethynyl) benzoic acid as a potential potent chemical pruner. 发现 4-(2-苯乙炔基)苯甲酸是一种潜在的强效化学修剪剂。

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-01-29 DOI: 10.1093/pcp/pcae142

Pingping Fang, Xiaofang Li, Mingxuan Li, Zhen Shi, Yusu Cheng, Jiazhong Shen, Chuanqing Zhang, Ting Sun, Kang Ning, Xinyang Wu, Dong Fang, Pei Xu

{"title":"Discovery of 4-(2-phenylethynyl) benzoic acid as a potential potent chemical pruner.","authors":"Pingping Fang, Xiaofang Li, Mingxuan Li, Zhen Shi, Yusu Cheng, Jiazhong Shen, Chuanqing Zhang, Ting Sun, Kang Ning, Xinyang Wu, Dong Fang, Pei Xu","doi":"10.1093/pcp/pcae142","DOIUrl":"10.1093/pcp/pcae142","url":null,"abstract":"Rocketing labor cost is a major challenge threatening agricultural sustainability and food security worldwide. The replacement of manual pruning of horticultural plants with chemical pruning has long been a goal for saving cost and reducing virus spreading. Here, guided by the structure-function relationship of allelochemical benzoic acid derivatives, we have identified 4-(2-phenylethynyl)-benzoicacid (PEBA) as a highly bioactive compound. PEBA arrested tomato seed germination at a concentration as low as 0.5 μM, and effectively suppressed lateral branching at 10-100 μM. In field conditions, a foliar spraying of 25 μM PEBA altered tomato plant architecture by repressing emergence and elongation of lateral buds, without significantly affecting plant growth and final fruit yield. Moreover, PEBA could also inhibit seed germination in cereals, albeit at a lower efficacy. The application of 500 μM and 750 μM PEBA profoundly curbed preharvest sprouting in wheat and rice. Acute toxicity analysis using silkworm and Trichogramma chilonis revealed that PEBA posed a low risk of toxicity. The persistence analysis further indicated that residue levels of PEBA in various plant organs were very low following applications. The inhibitory effect of PEBA on tomato seed germination was associated with the suppression of GA1 accumulation and reactive oxygen species generation, while its effect on lateral bud outgrowth were related to perturbations in the plant hormones and the modulation of genes involved in secondary metabolism and those encoding ABC transporters. Collectively, our findings demonstrate the potential of PEBA as a potent plant growth regulator with significant value for chemical pruning.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"75-88"},"PeriodicalIF":3.9,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142829760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Catalytic mechanism underlying the regiospecificity of coumarin-substrate transmembrane prenyltransferases in Apiaceae. 伞形科植物中香豆素底物跨膜前酰转移酶区域特异性的催化机理。

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-01-29 DOI: 10.1093/pcp/pcae134

Junwen Han, Ryosuke Munakata, Hironobu Takahashi, Takao Koeduka, Mayumi Kubota, Eiko Moriyoshi, Alain Hehn, Akifumi Sugiyama, Kazufumi Yazaki

{"title":"Catalytic mechanism underlying the regiospecificity of coumarin-substrate transmembrane prenyltransferases in Apiaceae.","authors":"Junwen Han, Ryosuke Munakata, Hironobu Takahashi, Takao Koeduka, Mayumi Kubota, Eiko Moriyoshi, Alain Hehn, Akifumi Sugiyama, Kazufumi Yazaki","doi":"10.1093/pcp/pcae134","DOIUrl":"10.1093/pcp/pcae134","url":null,"abstract":"Plant membrane-bound prenyltransferases (PTs) catalyze the transfer of prenyl groups to acceptor substrates, phenols, using prenyl diphosphates as the donor substrate. The presence of prenyl residues in the reaction products, prenylated phenols, is key to the expression of a variety of physiological activities. Plant PTs generally exhibit high specificities for both substrate recognition and prenylation sites, while the molecular mechanism involved in these enzymatic properties is largely unknown. In this study, we performed a systematic biochemical analysis to elucidate the catalytic mechanism responsible for the reaction specificity of plant PTs. Using two representative PTs, PsPT1 and PsPT2, from parsnip (Pastinaca sativa, Apiaceae), which differ only in the regiospecificity of the prenylation site, we performed domain swapping and site-directed mutagenesis of these PTs, followed by detailed enzymatic analysis combined with 3D modeling. As a result, we discovered the domains that control prenylation site specificity and further defined key amino acid residues responsible for the catalytic mechanism. In addition, we showed that the control mechanism of prenylation specificity revealed here is also highly conserved among coumarin-substrate PTs. These data suggest that the regulatory domain revealed here is commonly involved in prenylation regiospecificity in Apiaceae PTs.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"1-14"},"PeriodicalIF":3.9,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11775389/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142688657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Host responses to haustorium invasion of the stem parasitic plant, Cuscuta campestris, differ from responses to wounding. 寄主对茎寄生植物Cuscuta campestris吸器入侵的反应不同于对伤害的反应。

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-01-29 DOI: 10.1093/pcp/pcae130

Jihwan Park, Shota Yamamoto, Ayako Tsushima, Koh Aoki

{"title":"Host responses to haustorium invasion of the stem parasitic plant, Cuscuta campestris, differ from responses to wounding.","authors":"Jihwan Park, Shota Yamamoto, Ayako Tsushima, Koh Aoki","doi":"10.1093/pcp/pcae130","DOIUrl":"10.1093/pcp/pcae130","url":null,"abstract":"The parasitic mechanism employed by the stem parasitic plant, Cuscuta campestris, involves degradation of the host epidermis and intrusion of the cortical tissue of the host stem by a specialized organ called the haustorium. In host plants, the mechanical stimuli associated with this degradation and intrusion of host tissues is considered to be comparable to wounding. However, it has not yet been clarified whether parasitic invasion and wounding induce equivalent responses in host plants. In this study, we demonstrated that parasitic intrusion-induced responses that were comparable to wounding in the host plant, Arabidopsis thaliana, including upregulation of Arabidopsis NAC DOMAIN-CONTAINING PROTEIN 71 (ANAC071), which is a key transcription factor associated with wound repair, cell division, and vascular development. Despite these similarities, we found that the mechanism regulating the induction of cell division- and vascular development-related genes at the host-parasite interface differed from that associated with wound repair. Specifically, ANAC071 was not required for the induction of cell division-related genes, as their upregulation was observed in anac071/096/011 triple mutants as well as in wild-type host plants. We also found that neither auxin nor ethylene plays a significant role in inducing the expression of vascular development-related genes. Thus, the findings show that the mechanisms responsible for upregulating cell division- and vascular development-related genes differ between parasitic and wound repair responses.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"50-59"},"PeriodicalIF":3.9,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tetrapyrrole photoreceptors in photosynthetic organisms.

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-01-27 DOI: 10.1093/pcp/pcaf014

Tatsuru Masuda, Rei Narikawa, Yoshitaka Saga, Haruki Yamamoto, Matthew Terry, Yuichi Fujita

引用次数: 0

The Arabidopsis Basic-Helix-Loop-Helix Transcription Factor LRL1 Activates Cell Wall-Related Genes during Root Hair Development.

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-01-27 DOI: 10.1093/pcp/pcaf006

Shahrzad Haghir, Koh Yamada, Mariko Kato, Tomohiko Tsuge, Takuji Wada, Rumi Tominaga, Yohei Ohashi, Takashi Aoyama

{"title":"The Arabidopsis Basic-Helix-Loop-Helix Transcription Factor LRL1 Activates Cell Wall-Related Genes during Root Hair Development.","authors":"Shahrzad Haghir, Koh Yamada, Mariko Kato, Tomohiko Tsuge, Takuji Wada, Rumi Tominaga, Yohei Ohashi, Takashi Aoyama","doi":"10.1093/pcp/pcaf006","DOIUrl":"https://doi.org/10.1093/pcp/pcaf006","url":null,"abstract":"Lotus japonicus-ROOT HAIR LESS1-LIKE1 (LRL1) of Arabidopsis thaliana encodes a basic helix-loop-helix (bHLH) transcription factor (TF) involved in root hair development. Root hair development is regulated by an elaborate transcriptional network, in which GLABRA2 (GL2), a key negative regulator, directly represses bHLH TF genes, including LRL1 and ROOT HAIR DEFECTIVE6 (RHD6). Although RHD6 and its paralogous TFs have been shown to connect downstream to genes involved in cell morphological events such as endomembrane and cell wall modification, the network downstream of LRL1 remains elusive. We found that a mutation of LRL1 causes a short-root-hair phenotype and that this phenotype can be partially rescued by a transgene encoding a glucocorticoid receptor (GR) domain-fused LRL1, LRL1-GR, in the presence of glucocorticoids. Using this conditional rescue system, we identified 46 genes that are activated downstream of LRL1. Among these, cell wall-related genes were significantly enriched and many of them were found to be immediately downstream of LRL1 without de novo protein synthesis in between. We further analyzed three representative genes, PROLINE-RICH PROTEIN1 (PRP1), PRP3, and XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDOLASE12 (XTH12). Reporter gene analyses showed that these genes are specifically transcribed in root hair cells including those in the root-hypocotyl junction, and that their proteins were localized to the cell wall of elongating root hairs, root hair bulges, and root hair bulge-expecting loci. A T-DNA insertion mutant of PRP3 showed a moderate short-root-hair phenotype. Based on these results, LRL1 is likely to promote root hair development throughout the morphogenetic process by activating cell wall-related genes.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Subgenome-informed statistical modeling of transcriptomes in 25 common wheat accessions reveals cis- and trans- regulation architectures. 对25个普通小麦品种的转录组进行亚基因组统计建模，揭示了顺式和反式调控结构。

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-01-18 DOI: 10.1093/pcp/pcaf011

Yasuyuki Nomura, Moeko Okada, Toshiaki Tameshige, Shotaro Takenaka, Kentaro K Shimizu, Shuhei Nasuda, Atsushi J Nagano

{"title":"Subgenome-informed statistical modeling of transcriptomes in 25 common wheat accessions reveals cis- and trans- regulation architectures.","authors":"Yasuyuki Nomura, Moeko Okada, Toshiaki Tameshige, Shotaro Takenaka, Kentaro K Shimizu, Shuhei Nasuda, Atsushi J Nagano","doi":"10.1093/pcp/pcaf011","DOIUrl":"https://doi.org/10.1093/pcp/pcaf011","url":null,"abstract":"Common wheat is allohexaploid, where it is difficult to obtain homoeolog-distinguished transcriptome data. Lasy-Seq, a type of 3' RNA-seq, is a technology efficient at obtaining homoeolog-distinguished transcriptomes. Here we applied Lasy-Seq to obtain transcriptome data from the seedlings, second leaves, and root tips of 25 common wheat lines mainly from East Asia. Roots and seedlings were similar to each other in transcriptome profiles, but they were different from the leaves. We then asked how three homoeologous genes from different subgenomes (i.e., triads) show different levels of expression. Specifically, we examined the effects of subgenomes, lines and their interaction on the expression levels of each homoeolog triad, separately in each tissue. Of the 19,805 homoeolog triads, 51-55% showed significant effect of subgenome, suggesting cis-regulation, whereas 24-30% showed significant effect line, suggesting trans-regulation. We also found that 7.7-9.0% triads showed significant effects of the interaction. Hierarchical clustering and co-trans regulation network analysis of homoeolog triads revealed that the patterns of expression polymorphisms among the lines were shared in different genes. Our results also implied that expression variation between lines is caused by changes in a smaller number of common trans-factors. We performed GO term enrichment analysis using newly annotated and substantially improved GO annotations, which revealed that GO terms related to each tissue type function were enriched in genes expressed in the leaves and roots. Our information provides fundamental knowledge for the future breeding of plants possessing complex gene regulatory networks such as common wheat.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evidence for Peroxisomal Redundancy among the Glucose-6-phosphate Dehydrogenase Isoforms of Arabidopsis thaliana. 拟南芥葡萄糖-6-磷酸脱氢酶异构体过氧化物酶体冗余的证据。

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-01-18 DOI: 10.1093/pcp/pcaf012

Loreen Linnenbrügger, Lennart Nico Doering, Louisa-Marlen Tägtmeyer, Kerstin Fischer, Antje von Schaewen

{"title":"Evidence for Peroxisomal Redundancy among the Glucose-6-phosphate Dehydrogenase Isoforms of Arabidopsis thaliana.","authors":"Loreen Linnenbrügger, Lennart Nico Doering, Louisa-Marlen Tägtmeyer, Kerstin Fischer, Antje von Schaewen","doi":"10.1093/pcp/pcaf012","DOIUrl":"https://doi.org/10.1093/pcp/pcaf012","url":null,"abstract":"The oxidative pentose phosphate pathway (OPPP) plays an important role for the generation of reducing power in all eukaryotes. In plant cells the OPPP operates in several cellular compartments, but as full cycle only in the plastid stroma where it is essential. As suggested by our recent results, OPPP reactions are also mandatory inside peroxisomes, at least during fertilisation. For the first enzyme of the OPPP, glucose-6-phosphate dehydrogenase (G6PD), so far only one Arabidopsis isoform (G6PD1) was shown to be directed to peroxisomes under specific circumstances. Since g6pd1 knock-out plants are viable, we aimed at elucidating potential redundancy regarding peroxisomal targeting among the other G6PD isoforms. Localisation studies of so far cytosolic annotated G6PD5 and G6PD6 (both ending -PTL>) using different reporter fusions of full-length versus the last 50 amino acids revealed that GFP-C-short versions are efficiently imported into peroxisomes. Modification of the final tripeptide to a canonical peroxisomal targeting signal type 1 (PTS1) also resulted in peroxisomal localisation of the full-length versions, and revealed that G6PD5/6 import may occur as homo- or heterodimer. Interestingly, the new non-canonical PTS1 motif is highly conserved among the cytosolic G6PD isoforms of the Angiosperms, whereas members of the Poaceae (rice, maize) possess two variants, one ending with an additional amino acid (-PTLA>), and the other extended by a stronger PTS1 motif. From both evolutionary and physiological perspectives, we postulate that import as homo- and heterodimer restricted the acquisition of more efficient peroxisomal targeting motifs to leave some G6PDH activity to the cytosol.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0