Plant and Cell Physiology最新文献_第2页

Exploring the formation and permeability of plasmodesmata in the liverwort, Marchantia polymorpha. 多形地茅（Marchantia polymorpha）间连丝的形成及通透性研究。

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-03-31 DOI: 10.1093/pcp/pcae153

Chia-Yun Hsu, Chia-Hsuan Hsu, Hui-Yu Chang, Kuan-Ju Lu

{"title":"Exploring the formation and permeability of plasmodesmata in the liverwort, Marchantia polymorpha.","authors":"Chia-Yun Hsu, Chia-Hsuan Hsu, Hui-Yu Chang, Kuan-Ju Lu","doi":"10.1093/pcp/pcae153","DOIUrl":"10.1093/pcp/pcae153","url":null,"abstract":"Plasmodesmata are cell-wall-embedded channels that evolved in the common ancestor of land plants to increase cell-to-cell communication. Whether all the fundamental properties of plasmodesmata emerged and were inherited in all land plants at the same time is unknown. Here, we show that the bryophyte Marchantia polymorpha (a nonvascular plant) forms mostly simple plasmodesmata in early-developing gemmae. The complexity of plasmodesmata increases during gemma maturation, and complex plasmodesmata with enlarged cavities are majorly observed in thalli. In contrast to vascular plants, whose simple plasmodesmata can transport monomeric fluorescent proteins, plasmodesmata in M. polymorpha limited their permeability before the juvenile-to-adult transition. In support, callose, a known polysaccharide regulating plasmodesmata permeability in vascular plants, accumulated in most of the M. polymorpha tissues examined. Furthermore, we found that in the apical meristematic region, plasmodesmata allowed the transport of monomeric fluorescent proteins, and this relaxation might correlate with the lower accumulation of callose. Taken together, our study suggests that certain plasmodesmata properties, such as complexity progression and callose accumulation, may have evolved before the divergence between vascular and nonvascular plants.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"333-346"},"PeriodicalIF":3.9,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142953956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Poplar leaf bud resin metabolomics: seasonal profiling of leaf bud chemistry in Populus trichocarpa provides insight into resin biosynthesis. 杨树叶芽树脂代谢组学：杨树叶芽化学的季节性分析为树脂生物合成提供了见解。

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-03-31 DOI: 10.1093/pcp/pcae149

Eerik-Mikael Piirtola, David P Overy, C Peter Constabel

{"title":"Poplar leaf bud resin metabolomics: seasonal profiling of leaf bud chemistry in Populus trichocarpa provides insight into resin biosynthesis.","authors":"Eerik-Mikael Piirtola, David P Overy, C Peter Constabel","doi":"10.1093/pcp/pcae149","DOIUrl":"10.1093/pcp/pcae149","url":null,"abstract":"Trees in the genus Populus synthesize sticky and fragrant resins to protect dormant leaf buds during winter. These resins contain diverse phenolic metabolites, in particular, hydroxycinnamate esters and methylated flavonoids. Populus trichocarpa leaf bud resin is characterized by methylated dihydrochalcone aglycones. To determine how the resin profile is influenced by seasonal changes, P. trichocarpa lateral leaf bud extracts and secreted surface resin were collected monthly over a 1-year cycle. The dihydrochalcones in both sets of extracts were quantified using ultrahigh pressure liquid chromatography-mass spectrometry (UPLC-MS), and other chemical changes were monitored using nontargeted metabolomics by UPLC-high-resolution MS (UPLC-HRMS). The results indicate that the dihydrochalcone content changes over the seasons and that biosynthesis occurs concomitant with bud development in the summer months. Nontargeted metabolomics data confirmed a pattern of dramatic changes in the summer and further suggested additional periods of substantive biochemical change in the resin. While overall patterns of surface-extracted resin matched those of whole bud extracts, some of the dynamics were shifted in the surface resin samples. This study provides the basis for the use of dihydrochalcones and other identified resin components as metabolic markers for more detailed investigations of resin biosynthesis, secretion, and movement to the bud surface.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"291-303"},"PeriodicalIF":3.9,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11957272/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Involvement of MID1-COMPLEMENTING ACTIVITY 1 encoding a mechanosensitive ion channel in prehaustorium development of the stem parasitic plant Cuscuta campestris. 编码机械敏感离子通道的MID-1互补活性1在茎寄生植物菟丝子吸器前发育中的作用

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-03-31 DOI: 10.1093/pcp/pcaf009

Jihwan Park, Kyo Morinaga, Yuma Houki, Ayako Tsushima, Koh Aoki

{"title":"Involvement of MID1-COMPLEMENTING ACTIVITY 1 encoding a mechanosensitive ion channel in prehaustorium development of the stem parasitic plant Cuscuta campestris.","authors":"Jihwan Park, Kyo Morinaga, Yuma Houki, Ayako Tsushima, Koh Aoki","doi":"10.1093/pcp/pcaf009","DOIUrl":"10.1093/pcp/pcaf009","url":null,"abstract":"Parasitic plants pose a substantial threat to agriculture as they attack economically important crops. The stem parasitic plant Cuscuta campestris invades the host's stem with a specialized organ referred to as the haustorium, which absorbs nutrients and water from the host. Initiation of the parasitic process in C. campestris requires mechanical stimuli to its stem. However, the mechanisms by which C. campestris perceives mechanical stimuli are largely unknown. Previous studies have shown that mechanosensitive ion channels (MSCs) are involved in the perception of mechanical stimuli. To examine if MSCs are involved in prehaustorium development upon tactile stimuli, we treated C. campestris plants with an MSC inhibitor, GsMTx-4, which resulted in a reduced density of prehaustoria. To identify the specific MSC gene involved in prehaustorium development, we analyzed the known functions and expression patterns of Arabidopsis MSC genes and selected MID1-COMPLEMENTING ACTIVITY 1 (MCA1) as a primary candidate. The MSC activity of CcMCA1 was confirmed by its ability to complement the phenotype of a yeast mid1 mutant. To evaluate the effect of CcMCA1 silencing on prehaustorium development, we performed host-induced gene silencing using Nicotiana tabacum plants that express an artificial microRNA-targeting CcMCA1. In the CcMCA1-silenced C. campestris, the number of prehaustoria per millimeter of stem length decreased, and the interval length between prehaustoria increased. Additionally, the expression levels of known genes involved in prehaustorium development, such as CcLBD25, decreased significantly in the CcMCA1-silenced plants. The results suggest that CcMCA1 is involved in prehaustorium development in C. campestris.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"400-410"},"PeriodicalIF":3.9,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11957263/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Arabidopsis basic-helix-loop-helix transcription factor LRL1 activates cell wall-related genes during root hair development.

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-03-31 DOI: 10.1093/pcp/pcaf006

Shahrzad Haghir, Koh Yamada, Mariko Kato, Tomohiko Tsuge, Takuji Wada, Rumi Tominaga, Yohei Ohashi, Takashi Aoyama

{"title":"The Arabidopsis basic-helix-loop-helix transcription factor LRL1 activates cell wall-related genes during root hair development.","authors":"Shahrzad Haghir, Koh Yamada, Mariko Kato, Tomohiko Tsuge, Takuji Wada, Rumi Tominaga, Yohei Ohashi, Takashi Aoyama","doi":"10.1093/pcp/pcaf006","DOIUrl":"10.1093/pcp/pcaf006","url":null,"abstract":"Lotus japonicus-ROOT HAIR LESS1-LIKE-1 (LRL1) of Arabidopsis thaliana encodes a basic helix-loop-helix (bHLH) transcription factor (TF) involved in root hair development. Root hair development is regulated by an elaborate transcriptional network, in which GLABRA2 (GL2), a key negative regulator, directly represses bHLH TF genes, including LRL1 and ROOT HAIR DEFECTIVE6 (RHD6). Although RHD6 and its paralogous TFs have been shown to connect downstream to genes involved in cell morphological events, such as endomembrane and cell wall modification, the downstream network of LRL1 remains elusive. We found that a mutation of LRL1 causes a short-root hair phenotype and that this phenotype can be partially rescued by a transgene encoding a glucocorticoid receptor (GR) domain-fused LRL1, LRL1-GR, in the presence of glucocorticoids. Using this conditional rescue system, we identified 46 genes that are activated downstream of LRL1. Among these, the cell wall-related genes were significantly enriched and many of them were found to be immediately downstream of LRL1 without de novo protein synthesis in between. We further analyzed three representative genes, PROLINE-RICH PROTEIN1 (PRP1), PRP3, and XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDOLASE12 (XTH12). Reporter gene analyses showed that these genes are specifically transcribed in root hair cells including those in the root-hypocotyl junction, and that their proteins were localized to the cell wall of elongating root hairs, root hair bulges, and root hair bulge-expecting loci. A T-DNA insertion mutant of PRP3 showed a moderate short-root hair phenotype. Based on these results, LRL1 is likely to promote root hair development throughout the morphogenetic process by activating cell wall-related genes.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"384-399"},"PeriodicalIF":3.9,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A genetic modification strategy with dual benefit for use in a unicellular alga.

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-03-31 DOI: 10.1093/pcp/pcaf024

Shin-Ya Miyagishima

引用次数: 0

Divergent control of seed germination by cytokinins in weedy broomrapes and witchweeds.

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-03-24 DOI: 10.1093/pcp/pcaf032

Guillaume Brun, Estelle Billard, Adéla Hýlová, Grégory Montiel, Lenka Plačková, Karel Doležal, Virginie Puech-Pagès, Philippe Simier, Susann Wicke, Lukáš Spíchal, Philippe Delavault, Jean-Bernard Pouvreau

{"title":"Divergent control of seed germination by cytokinins in weedy broomrapes and witchweeds.","authors":"Guillaume Brun, Estelle Billard, Adéla Hýlová, Grégory Montiel, Lenka Plačková, Karel Doležal, Virginie Puech-Pagès, Philippe Simier, Susann Wicke, Lukáš Spíchal, Philippe Delavault, Jean-Bernard Pouvreau","doi":"10.1093/pcp/pcaf032","DOIUrl":"https://doi.org/10.1093/pcp/pcaf032","url":null,"abstract":"Broomrapes (Phelipanche and Orobanche spp.) and witchweeds (Striga spp.) are parasitic weeds that are increasingly threatening crops worldwide. Seeds of these species rely on host-derived signals such as strigolactones to germinate. While cytokinins were also reported as germination inducers of witchweeds, their role during germination of broomrapes remains unexplored. Our study shows that some but not all cytokinins stimulate Striga hermonthica germination independently of strigolactones, and that high concentrations of bioactive cytokinins trigger Striga seedlings to differentiate into fully extruded embryo-like structures. In contrast, cytokinin free bases but not ribosylated or glycosylated conjugates are extremely potent inhibitors of broomrapes germination. Germination inhibition upon the cytokinin signaling inhibitor PI-55 and inhibitor of cytokinin degradation INCYDE suggest that the cytokinin perception and degradation machinery is conserved in parasitic weeds. In Phelipanche ramosa, gene expression analyses combined with targeted quantification of cytokinin contents revealed that strigolactones first induce an increase in ABA catabolism, then a modification of the cytokinin endogenous pool in favor of inactive conjugates. Overall, this study provides valuable insights into the hormonal interplay governing seed germination in broomrapes and witchweeds, paving the way for future studies aimed at developing novel strategies for parasitic weed control.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143701380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular pH, cell length and cell differentiation do not firmly correlate across Arabidopsis root tissues.

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-03-24 DOI: 10.1093/pcp/pcaf031

H Nicholay Diaz-Ardila, Christian S Hardtke

引用次数: 0

The phytochrome B signaling regulates salt-mediated seedling growth in the dark.

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-03-19 DOI: 10.1093/pcp/pcaf029

Peipei Qi, Weiping Mo, Rongcheng Lin

{"title":"The phytochrome B signaling regulates salt-mediated seedling growth in the dark.","authors":"Peipei Qi, Weiping Mo, Rongcheng Lin","doi":"10.1093/pcp/pcaf029","DOIUrl":"https://doi.org/10.1093/pcp/pcaf029","url":null,"abstract":"Light is an essential environmental factor that facilitates the robust upward growth of post-germinative seedlings emerging from buried seeds that is partly mediated by the photoreceptors. Salinity stress hampers plant growth and development and reduces yield. However, the involvement and regulatory role of photoreceptors and light signaling factors to salt stress are large unknown. Here, we report that mutants of the phytochrome B (phyB) photoreceptor showed reduced sensitivity to salt-inhibited hypocotyl elongation in darkness, and that PHYTOCHROME INTERACTING FACTOR 3 (PIF3) acts downstream of phyB in regulating this process in Arabidopsis thaliana. We show that SALT OVERLY SENSITIVE 2 (SOS2) regulates phyB protein accumulation under salt stress in darkness. Surprisingly, salt treatment induces phyB nuclear body formation in darkness. Moreover, we found that the phosphorylation at residue Ser-86 of phyB is essential for its function, and the scaffold protein 14-3-3κ is involved in the regulation of phyB under salt stress in darkness. Our study reveals a regulatory role of the phyB-PIF3 module in mediating post-germination growth in darkness in response to salt stress.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expanded Functional Roles Of R2R3-MYB (S6) Transcription Factors In Balancing Phenylpropanoid And Phenolamide Pathway In Solanaceae.

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-03-13 DOI: 10.1093/pcp/pcaf028

Vincenzo D'Amelia, Anna Lisa Piccinelli, Teresa Docimo, Valerio Cirillo, Albino Maggio, Pasquale Chiaiese, Marco Possenti, Fabio D'Orso, Annalisa Staiti, Riccardo Aversano, Domenico Carputo

{"title":"Expanded Functional Roles Of R2R3-MYB (S6) Transcription Factors In Balancing Phenylpropanoid And Phenolamide Pathway In Solanaceae.","authors":"Vincenzo D'Amelia, Anna Lisa Piccinelli, Teresa Docimo, Valerio Cirillo, Albino Maggio, Pasquale Chiaiese, Marco Possenti, Fabio D'Orso, Annalisa Staiti, Riccardo Aversano, Domenico Carputo","doi":"10.1093/pcp/pcaf028","DOIUrl":"https://doi.org/10.1093/pcp/pcaf028","url":null,"abstract":"Events of duplication and neo/subfunctionalization have significantly expanded the functional roles of R2R3 MYB transcription factors in plants. In a previous study, we demonstrated that two paralogous R2R3 MYBs from Solanum tuberosum and S. commersonii, AN1 and AN2 respectively, induce anthocyanin pigmentation to varying extents when transiently overexpressed. However, questions related to the distinct functions of these genes remained unanswered. In this study, we further investigated these genes by comparing transgenic tobacco plants that constitutively overexpress AN1 and AN2. We observed that differences between AN1 and AN2 not only influenced plant pigmentation but also impacted the structural features of vascular tissues. Both genes promoted the accumulation of phenolamides; however, AN1 showed a stronger capacity to regulate the phenylpropanoid pathway. In addition, our results suggested a potential role for AN2 in regulating additional biological processes potentially involved in vascular development, as indicated by GUS promoter localization study. Collectively, these results shed new light on the potentially ancestral functions of these R2R3 MYB genes, extending their known impact beyond anthocyanin biosynthesis.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fine-tuned terpene synthase gene expression, functional promiscuity, and subcellular localization: Implications for the evolution of complex floral volatile bouquet in Caladenia orchids.

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-03-08 DOI: 10.1093/pcp/pcaf026

Fei Zhou, Yanan Zhao, James Perkins, Haiyang Xu, Eran Pichersky, Rod Peakall, Darren C J Wong

{"title":"Fine-tuned terpene synthase gene expression, functional promiscuity, and subcellular localization: Implications for the evolution of complex floral volatile bouquet in Caladenia orchids.","authors":"Fei Zhou, Yanan Zhao, James Perkins, Haiyang Xu, Eran Pichersky, Rod Peakall, Darren C J Wong","doi":"10.1093/pcp/pcaf026","DOIUrl":"10.1093/pcp/pcaf026","url":null,"abstract":"Chemically mediated floral volatile signals are crucial for pollinator attraction across angiosperms. However, beyond model plant systems, the molecular mechanisms underpinning their tissue-specific biosynthesis, regulation, and emission are still poorly understood. In this study of a food-deceptive insect pollinated orchid (Caladenia denticulata), we elucidated the molecular basis of α-pinene biosynthesis - the major floral volatile emitted by this species and diverse lower abundance monoterpenes and sesquiterpenes. To achieve this, we combined comparative transcriptomics between active glandular trichome-rich sepal tips and labellum and non-active remaining flower tissues, floral volatile headspace profiling, phylogenetic analysis of a multigene family, and protein functional assays. We found (1) multiple branch points of the terpene synthase (TPS) biosynthetic pathway were highly expressed and co-ordinately upregulated in the active floral tissues compared to non-active ones, (2) the monoterpene synthase CdTPS-b3 underpinning α-pinene biosynthesis and a bona fide promiscuous TPS CdTPS-b4 that may contribute to the diverse array of low-abundance mono- and sesquiterpenes found in its flowers, and (3) dual localization (plastid and cytosol) of CdTPS-b3 and CdTPS-b4. Our findings highlight metabolic pathway specialization at multiple TPS pathway branch points supporting the biosynthesis and emission of α-pinene in C. denticulata flowers that are implicated in its generalist pollinator attraction. Furthermore, the complexity of diverse floral terpenes in Caladenia is likely mediated by finely tuned TPS gene expression, functional promiscuity, and subcellular localization. We predict that the combination of these three mechanisms underpin the evolution of multiple deceptive pollination strategies in Caladenia.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143582356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0