Plant and Cell Physiology最新文献_第3页

Rice Master Regulator 'HYR' Enhances Growth and Defense Mechanisms with Consequences for Fall Armyworm Growth and Host Selection.

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-03-06 DOI: 10.1093/pcp/pcaf025

Krishnarao Gandham, Julie Thomas, Awaiz Riaz, Devi Balakrishnan, Andy Pereira, Rupesh Kariyat

{"title":"Rice Master Regulator 'HYR' Enhances Growth and Defense Mechanisms with Consequences for Fall Armyworm Growth and Host Selection.","authors":"Krishnarao Gandham, Julie Thomas, Awaiz Riaz, Devi Balakrishnan, Andy Pereira, Rupesh Kariyat","doi":"10.1093/pcp/pcaf025","DOIUrl":"https://doi.org/10.1093/pcp/pcaf025","url":null,"abstract":"Rice (Oryza sativa L.), the staple food for half of the world's population, suffers heavy damage by insect herbivores, especially the emerging Fall Armyworm (FAW), Spodoptera frugiperda. HYR (HIGHER YIELD RICE), a master regulator of multiple biological pathways with an established gene regulatory network, has been found to improve rice yield to ~29% and tolerance to environmental stress. However, its impact on defense has not been explored. We hypothesized that, FAW would target HYR plants because of its vigorous growth, and lead to trade offs for defense. Through a series of experiments with HYR and its wild type, we show that HYR plants have enhanced below-ground growth, physiological traits, direct and indirect defense traits including leaf trichomes, wax and volatile organic compounds. To test possible phytohormone mediated defense signalling, we focussed on jasmonic acid and salicylic acid gene expression panel and found most of these genes are highly expressed in HYR when compared to its wildtype counterpart. Bioassays examining developmental milestones also revealed that HYR plants effectively deter FAW, and when force fed, caused negative effects. Collectively, our findings suggest that the master regulator HYR (Higher Yield Rice expressing) plants enhance growth and physiological traits, as well as physical and chemical defense mechanisms through co-ordinated defense gene expression, that deter herbivore feeding, growth, development and host selection.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143567968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chlorophyll Pigments and Their Synthetic Analogs. 叶绿素色素及其合成类似物。

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-02-28 DOI: 10.1093/pcp/pcae094

Hitoshi Tamiaki, Saki Kichishima

引用次数: 0

Engineering of Phycourobilin Synthase: PubS to a Two-Electron Reductase. 植物胭脂虫素合成酶工程：PubS 到双电子还原酶。

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-02-28 DOI: 10.1093/pcp/pcae098

Keita Miyake, Saya Iwata, Rei Narikawa

{"title":"Engineering of Phycourobilin Synthase: PubS to a Two-Electron Reductase.","authors":"Keita Miyake, Saya Iwata, Rei Narikawa","doi":"10.1093/pcp/pcae098","DOIUrl":"10.1093/pcp/pcae098","url":null,"abstract":"Phycourobilin:ferredoxin oxidoreductase (PubS) belongs to the ferredoxin-dependent bilin reductase (FDBR) family and catalyzes the reduction of the C15=C16 double bond, followed by the C4=C5 double bond of biliverdin IXα to produce phycourobilin. Among the diverse FDBR enzymes that catalyze site-specific reduction reactions of bilins, PubS lineage is the only one that reduces the C4=C5 double bond. This family can be broadly divided into four-electron reduction enzymes, which catalyze two successive two-electron reductions, such as PubS, and two-electron reduction enzymes, which catalyze a single two-electron reduction. The crystal structures of diverse FDBRs, excluding PubS, have unraveled that there are two distinct binding modes in the substrate-binding pocket. In this study, we focused on the arginine (Arg) residues that is considered crucial for substrate-binding mode in two-electron reduction enzymes. Through sequence alignment and comparison with the predicted structure of PubS, we identified a residue in PubS that corresponds to the Arg residue in the two-electron reduction enzymes. We further introduced mutations to avoid the steric hindrance associated with changes in the binding mode. Biochemical characterization of these variants showed that we successfully modified PubS from a four-electron reduction enzyme to a two-electron reduction enzyme with the accumulation of radicals. Our results provide insight into the molecular mechanisms of the chromophore binding mode and proton donation from acidic residues.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"229-237"},"PeriodicalIF":3.9,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11879140/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142352645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Light Color Regulation of Photosynthetic Antennae Biogenesis in Marine Phytoplankton. 海洋浮游植物光合触角生物发生的光色调节

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-02-28 DOI: 10.1093/pcp/pcae115

David M Kehoe, Avijit Biswas, Bo Chen, Louison Dufour, Théophile Grébert, Allissa M Haney, Kes Lynn Joseph, Indika Kumarapperuma, Adam A Nguyen, Morgane Ratin, Joseph E Sanfilippo, Animesh Shukla, Laurence Garczarek, Xiaojing Yang, Wendy M Schluchter, Frédéric Partensky

{"title":"Light Color Regulation of Photosynthetic Antennae Biogenesis in Marine Phytoplankton.","authors":"David M Kehoe, Avijit Biswas, Bo Chen, Louison Dufour, Théophile Grébert, Allissa M Haney, Kes Lynn Joseph, Indika Kumarapperuma, Adam A Nguyen, Morgane Ratin, Joseph E Sanfilippo, Animesh Shukla, Laurence Garczarek, Xiaojing Yang, Wendy M Schluchter, Frédéric Partensky","doi":"10.1093/pcp/pcae115","DOIUrl":"10.1093/pcp/pcae115","url":null,"abstract":"Photosynthesis in the world's oceans is primarily conducted by phytoplankton, microorganisms that use many different pigments for light capture. Synechococcus is a unicellular cyanobacterium estimated to be the second most abundant marine phototroph, with a global population of 7 × 1026 cells. This group's success is partly due to the pigment diversity in their photosynthetic light harvesting antennae, which maximize photon capture for photosynthesis. Many Synechococcus isolates adjust their antennae composition in response to shifts in the blue:green ratio of ambient light. This response was named type 4 chromatic acclimation (CA4). Research has made significant progress in understanding CA4 across scales, from its global ecological importance to its molecular mechanisms. Two forms of CA4 exist, each correlated with the occurrence of one of two distinct but related genomic islands. Several genes in these islands are differentially transcribed by the ambient blue:green light ratio. The encoded proteins control the addition of different pigments to the antennae proteins in blue versus green light, altering their absorption characteristics to maximize photon capture. These genes are regulated by several putative transcription factors also encoded in the genomic islands. Ecologically, CA4 is the most abundant of marine Synechococcus pigment types, occurring in over 40% of the population oceanwide. It predominates at higher latitudes and at depth, suggesting that CA4 is most beneficial under sub-saturating photosynthetic light irradiances. Future CA4 research will further clarify the ecological role of CA4 and the molecular mechanisms controlling this globally important form of phenotypic plasticity.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"168-180"},"PeriodicalIF":3.9,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142366327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tetrapyrrole photoreceptors in photosynthetic organisms.

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-02-28 DOI: 10.1093/pcp/pcaf014

Tatsuru Masuda, Rei Narikawa, Yoshitaka Saga, Haruki Yamamoto, Matthew Terry, Yuichi Fujita

引用次数: 0

Chlorophyll Degradation and Its Physiological Function. 叶绿素降解及其生理功能

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-02-28 DOI: 10.1093/pcp/pcae093

Ayumi Tanaka, Hisashi Ito

{"title":"Chlorophyll Degradation and Its Physiological Function.","authors":"Ayumi Tanaka, Hisashi Ito","doi":"10.1093/pcp/pcae093","DOIUrl":"10.1093/pcp/pcae093","url":null,"abstract":"Research on chlorophyll degradation has progressed significantly in recent decades. In the 1990s, the structure of linear tetrapyrrole, which is unambiguously a chlorophyll degradation product, was determined. From the 2000s until the 2010s, the major enzymes involved in chlorophyll degradation were identified, and the pheophorbide a oxygenase/phyllobilin pathway was established. This degradation pathway encompasses several steps: (i) initial conversion of chlorophyll b to 7-hydroxymethyl chlorophyll a, (ii) conversion of 7-hydroxymethyl chlorophyll a to chlorophyll a, (iii) dechelation of chlorophyll a to pheophytin a, (iv) dephytylation of pheophytin a to pheophorbide a, (v) opening of the macrocycle to yield a red chlorophyll catabolite (RCC) and (vi) conversion of RCC to phyllobilins. This pathway converts potentially harmful chlorophyll into safe molecules of phyllobilins, which are stored in the central vacuole of terrestrial plants. The expression of chlorophyll-degrading enzymes is mediated by various transcription factors and influenced by light conditions, stress and plant hormones. Chlorophyll degradation is differently regulated in different organs and developmental stages of plants. The initiation of chlorophyll degradation induces the further expression of chlorophyll-degrading enzymes, resulting in the acceleration of chlorophyll degradation. Chlorophyll degradation was initially considered the last reaction in senescence; however, chlorophyll degradation plays crucial roles in enhancing senescence, degrading chlorophyll-protein complexes, forming photosystem II and maintaining seed quality. Therefore, controlling chlorophyll degradation has important agricultural applications.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"139-152"},"PeriodicalIF":3.9,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142036756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR/Cas9-mediated mutagenesis of SEED FATTY ACID REDUCER genes significantly increased seed oil content in soybean. CRISPR/ cas9介导的种子脂肪酸还原基因诱变显著提高了大豆种子含油量。

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-02-28 DOI: 10.1093/pcp/pcae148

Wenying Liao, Runze Guo, Jie Li, Na Liu, Lixi Jiang, James Whelan, Huixia Shou

引用次数: 0

Letter to the Editor: Removal of B800 Bacteriochlorophyll a from Light-Harvesting Complex 3 of the Purple Photosynthetic Bacterium Rhodoblastus acidophilus. 致编辑的信：从紫色光合细菌嗜酸乳杆菌的光收集复合体 3 中去除 B800 菌体叶绿素 a。

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-02-28 DOI: 10.1093/pcp/pcae065

Yoshitaka Saga, Kohei Hamanishi, Shota Kawato

引用次数: 0

Phycocyanobilin Binding and Specific Amino Acid Residues Near The Chromophore Contribute To Orange Light Perception By The Dualchrome Phytochrome Region. 植物色素结合和色团附近的特定氨基酸残基有助于双色植物色素区感知橙色光。

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-02-28 DOI: 10.1093/pcp/pcae077

Mana Fukazawa, Keita Miyake, Hiroki Hoshino, Keiji Fushimi, Rei Narikawa

{"title":"Phycocyanobilin Binding and Specific Amino Acid Residues Near The Chromophore Contribute To Orange Light Perception By The Dualchrome Phytochrome Region.","authors":"Mana Fukazawa, Keita Miyake, Hiroki Hoshino, Keiji Fushimi, Rei Narikawa","doi":"10.1093/pcp/pcae077","DOIUrl":"10.1093/pcp/pcae077","url":null,"abstract":"A novel photoreceptor dualchrome 1 (DUC1), containing a fused structure of cryptochrome and phytochrome, was discovered in the marine green alga Pycnococcus provasolli. The DUC1 phytochrome region (PpDUC1-N) binds to the bilin (linear tetrapyrrole) chromophores, phytochromobilin (PΦB) or phycocyanobilin (PCB), and reversibly photoconverts between the orange-absorbing dark-adapted state and the far-red-absorbing photoproduct state. This contrasts with typical phytochromes, which photoconvert between the red-absorbing dark-adapted and far-red-absorbing photoproduct states. In this study, we examined the molecular mechanism of PpDUC1-N to sense orange light by identifying the chromophore species synthesized by P. provasolli and the amino acid residues within the PpDUC1-N responsible for sensing orange light in the dark-adapted state. We focused on the PcyA homolog of P. provasolli (PpPcyA). Coexpression with the photoreceptors followed by an enzymatic assay revealed that PpPcyA synthesized PCB. Next, we focused on the PpDUC1-N GAF domain responsible for chromophore binding and light sensing. Ten amino acid residues were selected as the mutagenesis target near the chromophore. Replacement of these residues with those conserved in typical phytochromes revealed that three mutations (F290Y/M304S/L353M) resulted in a 23-nm red shift in the dark-adapted state. Finally, we combined these constructs to obtain the PΦB-binding F290Y/M304S/L353M mutant and a 38-nm red shift was observed compared with the PCB-binding wild-type PpDUC1. The binding chromophore species and the key residues near the chromophore contribute to blue-shifted orange light sensing in the dark-adapted state of the PpDUC1-N.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"193-203"},"PeriodicalIF":3.9,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11879098/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141580651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genes for the Type-I Reaction Center and Galactolipid Synthesis are Required for Chlorophyll a Accumulation in a Purple Photosynthetic Bacterium. 紫色光合细菌叶绿素 a 的积累需要 I 型反应中心和半乳糖脂合成基因。

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-02-28 DOI: 10.1093/pcp/pcae076

Yusuke Tsukatani, Chihiro Azai, Tomoyasu Noji, Shigeru Kawai, Saori Sugimoto, Shigeru Shimamura, Yasuhiro Shimane, Jiro Harada, Tadashi Mizoguchi, Hitoshi Tamiaki, Shinji Masuda

{"title":"Genes for the Type-I Reaction Center and Galactolipid Synthesis are Required for Chlorophyll a Accumulation in a Purple Photosynthetic Bacterium.","authors":"Yusuke Tsukatani, Chihiro Azai, Tomoyasu Noji, Shigeru Kawai, Saori Sugimoto, Shigeru Shimamura, Yasuhiro Shimane, Jiro Harada, Tadashi Mizoguchi, Hitoshi Tamiaki, Shinji Masuda","doi":"10.1093/pcp/pcae076","DOIUrl":"10.1093/pcp/pcae076","url":null,"abstract":"Anoxygenic photosynthesis is diversified into two classes: chlorophototrophy based on a bacterial type-I or type-II reaction center (RC). Whereas the type-I RC contains both bacteriochlorophyll and chlorophyll, type-II RC-based phototrophy relies only on bacteriochlorophyll. However, type-II phototrophic bacteria theoretically have the potential to produce chlorophyll a by the addition of an enzyme, chlorophyll synthase, because the direct precursor for the enzyme, chlorophyllide a, is produced as an intermediate of BChl a biosynthesis. In this study, we attempted to modify the type-II proteobacterial phototroph Rhodovulum sulfidophilum to produce chlorophyll a by introducing chlorophyll synthase, which catalyzes the esterification of a diterpenoid group to chlorophyllide a thereby producing chlorophyll a. However, the resulting strain did not accumulate chlorophyll a, perhaps due to the absence of endogenous chlorophyll a-binding proteins. We further heterologously incorporated genes encoding the type-I RC complex to provide a target for chlorophyll a. Heterologous expression of type-I RC subunits, chlorophyll synthase and galactolipid synthase successfully afforded detectable accumulation of chlorophyll a in Rdv. sulfidophilum. This suggests that the type-I RC can work to accumulate chlorophyll a and that galactolipids are likely necessary for the type-I RC assembly. The evolutionary acquisition of type-I RCs could be related to prior or concomitant acquisition of galactolipids and chlorophylls.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"204-213"},"PeriodicalIF":3.9,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141727685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0