Plant and Cell Physiology最新文献_第3页

A Novel Alfalfa MsWRKY75 Transcription Factor Negatively Regulates Seed Germination via an ABA-Dependent Pathway Under Salinity Stress. 盐胁迫下苜蓿MsWRKY75转录因子通过aba依赖途径负调控种子萌发

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-09-24 DOI: 10.1093/pcp/pcaf119

Zhaoran Zhang, Haoyan Tang, Xinying Guo, Yingying Zheng, Linyao Wang, Ting Li, Yuguang Song, Wei Dong

{"title":"A Novel Alfalfa MsWRKY75 Transcription Factor Negatively Regulates Seed Germination via an ABA-Dependent Pathway Under Salinity Stress.","authors":"Zhaoran Zhang, Haoyan Tang, Xinying Guo, Yingying Zheng, Linyao Wang, Ting Li, Yuguang Song, Wei Dong","doi":"10.1093/pcp/pcaf119","DOIUrl":"https://doi.org/10.1093/pcp/pcaf119","url":null,"abstract":"Salinity stress severely impairs seed germination in plants. In this study, we identified MsWRKY75, a salt-responsive WRKY transcription factor from alfalfa (Medicago sativa L.), and characterized its role in modulating abscisic acid (ABA) signaling to regulate seed germination under salinity stress. Phylogenetic and structural analyses revealed that MsWRKY75, homologous to Medicago truncatula MtWRKY75, contains a conserved WRKYGQK motif and a C2H2-type zinc finger domain. Subcellular localization confirmed its nuclear localization, while yeast assays demonstrated transcriptional activation activity, supporting its function as a transcription factor. Overexpression of MsWRKY75 in Arabidopsis and M. truncatula led to enhanced suppression of seed germination under both salinity and ABA treatments, in a dose-dependent manner. Interestingly, MsWRKY75 did not affect ABA biosynthesis or catabolism, but specifically upregulated core components of the ABA signaling pathway. qRT-PCR analysis revealed strong induction of MtABI5 a key repressor of seed germination and its upstream receptor MtPYL4 in MsWRKY75-overexpressing lines. Yeast one-hybrid, dual-luciferase, and chromatin immunoprecipitation (ChIP) assays demonstrated that MsWRKY75 directly binds to the W-box cis-element within the MtABI5 promoter, activating its transcription. Mutation of the W-box motif abolished this interaction, confirming its essential role in transcriptional activation. Together, these results establish MsWRKY75 as a negative regulator of seed germination under salinity stress, acting through direct enhancement of ABA signaling. This study provides new mechanistic insights into ABA-mediated stress responses and identifies MsWRKY75 as a promising candidate for improving stress resilience through genetic engineering in legume crops.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145131952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Jasmonic Acid and Salicylic Acid Interact to Determine Spatial Regulation of Gene Expression Responses in Potato Leaf to Herbivory by Colorado Potato Beetle and Mechanical Wounding. 茉莉酸和水杨酸相互作用确定马铃薯叶片对草食甲虫和机械伤害的基因表达响应的空间调控。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-09-24 DOI: 10.1093/pcp/pcaf120

Valentina Levak, Tjaša Mahkovec Povalej, Karmen Pogačar, Katja Stare, Maja Zagorščak, Tim Hawkins, Joanne Robson, David Dobnik, Tjaša Lukan, Kristina Gruden

{"title":"Jasmonic Acid and Salicylic Acid Interact to Determine Spatial Regulation of Gene Expression Responses in Potato Leaf to Herbivory by Colorado Potato Beetle and Mechanical Wounding.","authors":"Valentina Levak, Tjaša Mahkovec Povalej, Karmen Pogačar, Katja Stare, Maja Zagorščak, Tim Hawkins, Joanne Robson, David Dobnik, Tjaša Lukan, Kristina Gruden","doi":"10.1093/pcp/pcaf120","DOIUrl":"https://doi.org/10.1093/pcp/pcaf120","url":null,"abstract":"We investigated the spatial dynamics of potato (Solanum tuberosum) responses to herbivory and mechanical wounding. We first followed the spatiotemporal response of jasmonic acid (JA)-signaling, known to be involved in the response. We generated two potato sensor lines: a JAZ degradation sensor and a downstream multicystatin transcriptional reporter. Both sensors revealed concentric, locally restricted responses on wounded leaves. Notably, JA-dependent gene expression was absent in cells immediately adjacent to the wound, whereas JAZ degradation spread continuously outward from the wound site. This pattern occurred after both herbivore attack and mechanical injury by the needle. To probe the mechanism, a salicylic acid (SA) reporter showed SA accumulation near the wound. Introducing the multicystatin reporter into SA-depleted NahG plants produced a uniform spread of multicystatin expression, confirming that SA attenuates the JA response in proximal cells. Together, these results show that a locally distinct, spatiotemporal SA-JA crosstalk shapes wound responses in potato, extending principles known from pathogen-plant interactions to herbivory and mechanical damage.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145131938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Nitric oxide delayed tomato fruit coloring by regulating chlorophyll- and carotenoid-related genes in a SlSPL6c-dependent manner. 更正：一氧化氮通过以slspl6c依赖的方式调节叶绿素和类胡萝卜素相关基因延迟番茄果实着色。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-09-23 DOI: 10.1093/pcp/pcaf118

引用次数: 0

Interplant signal transduction between dodder (Cuscuta) and their hosts. 菟丝子与寄主间的植物间信号转导。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-09-19 DOI: 10.1093/pcp/pcaf117

Jingxiong Zhang, Guojing Shen, Songkui Cui, Wentao Wang, Jianqiang Wu

引用次数: 0

Reduced Strigolactone Exudation as a Key Resistance Mechanism in Wild Carrots against Phelipanche aegyptiaca. 减少独角麦内酯分泌是野生胡萝卜对埃及褐斑虫抗性的关键机制。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-09-18 DOI: 10.1093/pcp/pcaf113

Sukhmanpreet Kaur, Mwafaq Ibdah, Riko Sakioka, Kyogo Nagano, Kaori Yoneyama, Philipp Simon, James Westwood, Dorothea Tholl

{"title":"Reduced Strigolactone Exudation as a Key Resistance Mechanism in Wild Carrots against Phelipanche aegyptiaca.","authors":"Sukhmanpreet Kaur, Mwafaq Ibdah, Riko Sakioka, Kyogo Nagano, Kaori Yoneyama, Philipp Simon, James Westwood, Dorothea Tholl","doi":"10.1093/pcp/pcaf113","DOIUrl":"https://doi.org/10.1093/pcp/pcaf113","url":null,"abstract":"Phelipanche aegyptiaca is a root parasitic plant that causes significant yield losses in many crops, including carrots (Daucus carota). This study investigates the resistance mechanisms of two wild carrot accessions, PI 21793 (Daucus glaber) and PI 341902 (Daucus littoralis), against Podalirius aegyptiaca compared to a cultivated carrot (P0114; D. carota). Wild carrots induced lower germination rates of P. aegyptiaca seeds and fewer successful tubercles, indicating both pre-attachment and partial post-attachment resistance mechanisms. Strigolactone analysis revealed significant quantitative differences between cultivated and wild carrots. While cultivated carrots exuded high levels of two strigolactones, one of which was putatively identified as the non-canonical strigolactone, 4-oxo-methyl-carlalactone (4-oxo-MeCLA), wild carrots released lower amounts of these compounds. Supplementation with the artificial strigolactone analog GR24 increased germination in P. aegyptiaca inoculated on wild carrots, suggesting that strigolactone deficiency and possibly altered composition are key pre-attachment resistance mechanisms. However, higher germination resulted in no significant improvement in tubercle development on wild carrots. Parasite seedlings showed necrosis-like symptoms at their attachment sites on wild carrot roots, indicating an additional post-attachment resistance mechanism. These findings provide new insights into strigolactone-mediated host resistance and highlight the potential of wild carrot accessions to contribute to the development of resistant cultivars against parasitic plants.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145092576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide identification and expression analysis Pvrboh genes reveal their potential roles in salt tolerance and calcium signaling. Pvrboh基因的全基因组鉴定和表达分析揭示了它们在盐耐受性和钙信号传导中的潜在作用。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-09-17 DOI: 10.1093/pcp/pcaf115

Ling Pan, Lirong Cai, Yang Lu, Di Peng

{"title":"Genome-wide identification and expression analysis Pvrboh genes reveal their potential roles in salt tolerance and calcium signaling.","authors":"Ling Pan, Lirong Cai, Yang Lu, Di Peng","doi":"10.1093/pcp/pcaf115","DOIUrl":"https://doi.org/10.1093/pcp/pcaf115","url":null,"abstract":"Salinity stress is a major environmental factor that limits plant growth and productivity. Understanding the mechanisms of plant salt tolerance is crucial for improving crop yields in saline soils. The Respiratory Burst Oxidases (Rboh) gene family triggers the production of reactive oxygen species (ROS), which act as a signaling molecule to regulate plant tolerance and development under saline conditions. However, a comprehensive analysis of the Rboh gene family in halophytic plants is largely unexplored. In this study, we characterized members of the Rboh gene family in Paspalum vaginatum, a typical halophyte. Evolutionary analysis revealed numerical differences in Pvrboh genes compared to those related species. Additionally, cis-acting elements related to plant hormones, light response, and plant development were identified in Pvrboh promoters. All Pvrboh genes were found to be membrane-localized and upregulated under high salinity, contributing to either root-sourced or leaf-sourced H2O2 production. Gene structural analysis and Ca2+ inhibitor experiments further indicated that Pvrboh4 and Pvrboh5 were closely associated with Ca2+ signaling, whereas Pvrboh6 did not exhibit such an association. Split-luciferase assay in N. benthamiana showed that PvRBOH5 strongly interacted with AtCPK5. Furthermore, a gene regulatory network analysis revealed multiple transcription factors from 32 distinct families that may regulate Pvrboh5 expression. These findings provide new insights into the role of Pvrboh genes in enhancing plant salt tolerance, offering potential targets for improving stress resilience in crops.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145092555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low-temperature-induced stress activates lipid deacylation at the sn-1 position in the cyanobacterium Synechocystis sp. PCC 6803. 低温诱导的应激激活了蓝细菌Synechocystis sp. PCC 6803中sn-1位点的脂质去酰化。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-09-17 DOI: 10.1093/pcp/pcaf116

Sumie Keta, Honoka Saruhashi, Kazutaka Ikeda, Nobuyuki Takatani, Ui Matsumoto, Kouji Kojima, Yuya Senoo, Tatsuo Omata, Makiko Aichi

{"title":"Low-temperature-induced stress activates lipid deacylation at the sn-1 position in the cyanobacterium Synechocystis sp. PCC 6803.","authors":"Sumie Keta, Honoka Saruhashi, Kazutaka Ikeda, Nobuyuki Takatani, Ui Matsumoto, Kouji Kojima, Yuya Senoo, Tatsuo Omata, Makiko Aichi","doi":"10.1093/pcp/pcaf116","DOIUrl":"https://doi.org/10.1093/pcp/pcaf116","url":null,"abstract":"Acyl-acyl carrier protein synthetase (Aas), involved in free fatty acid (FFA) recycling, is essential for growth of Synechocystis sp. PCC 6803 at 30 °C under the high-light (HL) stress conditions that activates lipid deacylation at the sn-2 position (400 μmol photons m-2 s-1), but not under low-light (LL) conditions (50 μmol photons m-2 s-1) Kojima et al. et al. (High-light-induced stress activates lipid deacylation at the sn-2 position in the cyanobacterium Synechocystis sp. PCC 6803. Plant Cell Physiol. 2022;63:82-91). Even under the LL conditions, however, Aas-deficient mutant cells grew much more slowly than WT cells at 22 °C. The mutant accumulated several times larger amounts of FFAs when cultivated at 22 °C than when cultivated at 30 °C, with C18 polyunsaturated FFAs comprising > 90% of the total FFAs. At 22 °C, the mutant cells also accumulated lysolipids derived from all four major lipid classes of cyanobacteria. The lysolipids were found to carry a C16 fatty acyl moiety. Since C18 and C16 fatty acids are esterified to the sn-1 and sn-2 positions, respectively, of membrane lipids in Synechocystis, the findings indicated that the low-temperature conditions activated lipid deacylation at the sn-1 position. In Synechococcus elongatus PCC 7942, whose membrane lipids carry mainly C16 fatty acids as acyl moieties and do not produce polyunsaturated fatty acids, low temperature activation of lipid deacylation was detected in an Aas-deficient mutant, but the amount of resulting FFAs was small, and no growth inhibition was observed at 22 °C. These results suggested that accumulation of toxic polyunsaturated FFAs was the cause of growth inhibition of the Synechocystis Aas mutant at 22 °C and that Aas is normally preventing accumulation of the toxic products.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145092566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multifaceted roles of U-box E3 ligases in plant development. U-Box E3连接酶在植物发育中的多方面作用

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-09-13 DOI: 10.1093/pcp/pcaf059

H N Karthik, Sourabh Parmar, Nilesh D Gawande, Subramanian Sankaranarayanan

引用次数: 0

Jacalin-induced cell proliferation during tissue-repair process in partially incised flowering stems of Arabidopsis thaliana. 拟南芥部分切开开花茎组织修复过程中jacalin诱导的细胞增殖。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-09-13 DOI: 10.1093/pcp/pcaf071

Afiifah Machfuudzoh, Ryo Koshiba, Takumi Higaki, Yusuke Ohba, Masashi Asahina, Shinobu Satoh, Hiroaki Iwai

{"title":"Jacalin-induced cell proliferation during tissue-repair process in partially incised flowering stems of Arabidopsis thaliana.","authors":"Afiifah Machfuudzoh, Ryo Koshiba, Takumi Higaki, Yusuke Ohba, Masashi Asahina, Shinobu Satoh, Hiroaki Iwai","doi":"10.1093/pcp/pcaf071","DOIUrl":"10.1093/pcp/pcaf071","url":null,"abstract":"Stem wounding is one of the most common abiotic stresses affecting plant development and productivity. During tissue-repair process of partially incised flowering stems of Arabidopsis thaliana, wounded tissues heal by a cascade of processes including tissue adhesion, cell dedifferentiation, cell proliferation, and subsequently tissue redifferentiation leading to the formation of new differentiated tissue. Cell proliferation is a key process in the recovery of wounded tissues. Nonetheless, the mechanisms regulating the initiation of cell proliferation remain elusive. In this study, we identified that jacalin, a lectin derived from the seeds of jackfruits (Artocarpus integrifolia), plays a role in inducing cell proliferation. Adding jacalin on the incision gap of the incised stem caused enhanced cell proliferation. One of A. thaliana JACALIN-RELATED LECTINS, JAL34, was highly expressed especially at 1 to 5 days after incision (DAI). Histological analysis of the defected mutant of jal34 showed an inactive cell proliferation. Our results suggest that JAL34 is involved in initiating cell proliferation during tissue-repair process.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"1229-1239"},"PeriodicalIF":4.0,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144542047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A herbivore-induced salicylic acid carboxyl methyl transferase produces methyl salicylate in tomato to mediate defense signaling and deter pests. 草食诱导的水杨酸羧基甲基转移酶在番茄中产生水杨酸甲酯介导防御信号和抑制害虫。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-09-13 DOI: 10.1093/pcp/pcaf066

Ariel Sorg, Jorrel Mendoza, Hui Liu, Kristin Roy, Zachary Gorman, Denise M Tieman, Cindy McKenzie, Gilles J Basset, Anna K Block

{"title":"A herbivore-induced salicylic acid carboxyl methyl transferase produces methyl salicylate in tomato to mediate defense signaling and deter pests.","authors":"Ariel Sorg, Jorrel Mendoza, Hui Liu, Kristin Roy, Zachary Gorman, Denise M Tieman, Cindy McKenzie, Gilles J Basset, Anna K Block","doi":"10.1093/pcp/pcaf066","DOIUrl":"10.1093/pcp/pcaf066","url":null,"abstract":"Methyl salicylate (MeSA) is a component of the herbivore-induced volatile blend of tomato (Solanum lycopersicum), and an intermediate in long distance salicylic acid (SA) signaling. To investigate the role of MeSA in interactions between tomato and the lepidopteran pests Helicoverpa zea and Manduca sexta, we used transgenic tomato lines that were antisense (AS) or overexpressors (OE) of the tomato SA carboxyl methyl transferase SlSAMT1, which under- or over-produce MeSA, respectively. Transgenic AS plants produced less MeSA and were more susceptible to both insects compared to wild-type plants, while OE plants had elevated MeSA production but displayed increased resistance only to M. sexta. Pairwise choice and toxicity assays revealed that MeSA itself impacted the larvae as a deterrent rather than a toxin, and metabolic profiling indicated that increased susceptibility in the AS lines and resistance in the OE lines may be related to altered jasmonic acid and ABA mediated herbivore-induced defense signaling, and modified production of inducible defense compounds such as flavonoids and phenolamides. To expand beyond Lepidoptera, the settling preference of the piercing-sucking insect Bemisia tabaci was also examined and it displayed a strong preference for lines with less SlSAMT1 expression. These data indicate that herbivore-inducible SlSAMT1 expression and its associated MeSA production play an important and complex role in the defense of tomato against insect pests.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"1192-1204"},"PeriodicalIF":4.0,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144576045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0