Plant and Cell Physiology最新文献

{"title":"Sorghum bicolor membrane steroid binding protein 1 can bind heme and remodel ER membranes.","authors":"Khuanpiroon Ratanasopa, Rocio Ochoa-Fernandez, Silas B Mellor, Valentina Travaglia, Kasper Hinz, Pernille S Tuelung, Tomas Laursen","doi":"10.1093/pcp/pcaf160","DOIUrl":"10.1093/pcp/pcaf160","url":null,"abstract":"<p><p>Plant membrane steroid binding proteins (MSBPs) belong to the membrane-associated progesterone receptors (MAPRs), which is present in all eukaryotic kingdoms. Plant MSBPs have been shown to regulate the function of cytochrome P450 enzymes, bind different steroidal compounds and confer salt tolerance. However, the exact molecular function of plant MSBPs remains elusive. Here, we perform a phylogenetic analysis of the six MAPR genes encoded in the Sorghum bicolor genome. Of these, four group into a distinct MSBP clade characterized by being N-terminally membrane anchored followed by a cytochrome b5 domain and an extended disordered C-terminal. Biophysical characterization of SbMSBP1 demonstrates that this protein can bind heme, which leads to dimerization potentially through a heme-heme stacking mechanism. Using untargeted proteomics, We further show that MSBPs are upregulated in both root and shoot tissues upon exposure to salt stress. Based on weighted gene co-expression network analysis, we find that SbMSBP1 abundance clusters with endoplasmic reticulum (ER) remodeling and vesicle transport proteins. We further show that overexpression of SbMSBP1 in S. bicolor protoplasts and tobacco results in formation of structures consistent with organized smooth ER. Our data indicate that SbMSBP1 functions to remodel ER membranes, which may be directly linked to a functional role in stress resilience toward both biotic and abiotic stresses and furthermore could serve as a useful tool for metabolic engineering of ER-scaffolded biosynthetic pathways.</p>","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"315-328"},"PeriodicalIF":4.0,"publicationDate":"2026-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13078165/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145669565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mutations in Arabidopsis SR subfamily genes affect flowering time and the splicing of multiple floral regulators including CONSTANS and FLOWERING LOCUS C.","authors":"Di Zhang, Mingkun Huang, Min Li, Mo-Xian Chen, Ying-Gao Liu, Jianhua Zhang","doi":"10.1093/pcp/pcaf158","DOIUrl":"10.1093/pcp/pcaf158","url":null,"abstract":"<p><p>Serine/arginine-rich (SR) proteins are essential splicing factors in animals, where their mutations often cause widespread splicing defects and carcinogenesis. The plant SR subfamily proteins are homologous to the well-studied human SR splicing factor 1, but their roles remain unclear. Here, we characterize the Arabidopsis SR subfamily genes: SR30, SR34, SR34a, and SR34b. We show that GFP-tagged SR30, SR34, and SR34a co-localized with the spliceosomal protein U1-70K in speckled nuclear structures. To explore their physiological roles, we constructed a series of multiple mutants. Interestingly, the quadruple mutant displayed delayed flowering under long-day conditions but accelerated flowering under short-day conditions. Under long days, SR30, SR34, and SR34a function redundantly, as delayed flowering was observed only when all three were simultaneously disrupted. Under short days, SR34a plays a predominant role, being both necessary and sufficient to maintain normal flowering. RNA sequencing and quantitative reverse transcription PCR (qPCR) analysis revealed altered splicing of multiple flowering time regulators, including CONSTANS (CO) and FLOWERING LOCUS C. Particularly, increased production of an inhibitory CO isoform correlated with delayed flowering under long days, which was rescued by CO.1 overexpression, suggesting the phenotype was linked to CO missplicing. Overall, our findings uncover the roles of SR subfamily genes in floral transition, highlighting the physiological significance of splicing regulation in plants.</p>","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"301-314"},"PeriodicalIF":4.0,"publicationDate":"2026-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145605589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Sorghum bicolor membrane steroid binding protein 1 can bind heme and remodel ER membranes.","authors":"","doi":"10.1093/pcp/pcag008","DOIUrl":"10.1093/pcp/pcag008","url":null,"abstract":"","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"389"},"PeriodicalIF":4.0,"publicationDate":"2026-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13078156/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146053085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salicylic acid and the unique TGA transcription factor controls plant immunity against Pseudomonas syringae in Marchantia polymorpha.","authors":"Santiago Michavila, Loreto Espinosa-Cores, Sophie Kneeshaw, Salvador Torres, Jitka Siroka, Angel M Zamarreno, Marina Gonzalez-Zuloaga, Jose M Garcia-Mina, Ondrej Novak, Roberto Solano, Selena Gimenez-Ibanez","doi":"10.1093/pcp/pcaf164","DOIUrl":"10.1093/pcp/pcaf164","url":null,"abstract":"<p><p>Land plants have co-evolved with microorganisms since their transition to a terrestrial habitat, around 500 million years ago. In angiosperms, salicylic acid (SA) activates plant immunity against hemibiotrophic pathogens through TGACG-motif-binding (TGA) transcription factors, which bind to the promoter of SA-responsive loci, such as pathogenesis-related (PR) genes, to enforce plant immunity. While those mechanisms are well-known in flowering plants, our understanding in bryophytes remains limited, as genetic evidences for the role of SA during plant immunity are still missing. Here, we explore the interaction between Marchantia polymorpha and the bacterium Pseudomonas syringae to gain insights into the evolutionary immune function of SA during bryophyte-pathogen interactions. We combined transcriptomic profiling of P. syringae-infected Marchantia with the generation of SA-deficient plants in this liverwort by overexpressing the bacterial NahG gene, a SA-degrading enzyme. Our results indicate that the P. syringae-induced transcriptional footprint is enriched in SA-responsive genes and that SA-deficient Marchantia NahG plants are compromised in immune responses against P. syringae. We show that the unique MpTGA is essential for controlling resistance against Pseudomonas. Further transcriptional analyses into the coregulatory network controlled by SA and MpTGA indicate that an SA/MpTGA module activates plant defence responses through a variety of MpPRs, enriched in the regulation of class III of secretory peroxidases belonging to the MpPR9 subfamily during the early defensive response against P. syringae. Altogether, our data demonstrate the functional conservation of SA as an immune hormone and underpin the existence of a SA/MpTGA-regulated transcriptional cluster driving resistance against Pseudomonas in Marchantia.</p>","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"329-345"},"PeriodicalIF":4.0,"publicationDate":"2026-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13078155/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145696245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MarpolBase: genome database for Marchantia polymorpha featuring high quality reference genome sequences.","authors":"Yasuhiro Tanizawa, Takako Mochizuki, Masaru Yagura, Mika Sakamoto, Takatomo Fujisawa, Shogo Kawamura, Eita Shimokawa, Shohei Yamaoka, Ryuichi Nishihama, John L Bowman, Frédéric Berger, Katsuyuki T Yamato, Takayuki Kohchi, Yasukazu Nakamura","doi":"10.1093/pcp/pcaf159","DOIUrl":"10.1093/pcp/pcaf159","url":null,"abstract":"<p><p>The liverwort Marchantia polymorpha is a key model organism for understanding land plant evolution, development, and gene regulation. To support the growing demand for high-quality genomic resources, we present MarpolBase, a comprehensive and integrated genome database that hosts newly assembled, high-accuracy reference genomes for both the male Tak-1 and female Tak-2 accessions, designated as ver. 7.1 reference genomes. These new assemblies, generated using PacBio HiFi long-read sequencing, represent nearly telomere-to-telomere chromosome-level genomes, with improvements in assembly continuity, annotation accuracy, and structural resolution-especially for repeat-rich regions and sex chromosomes. MarpolBase offers not only access to genome sequences and gene annotations but also provides a unified platform for data exploration, comparative analysis, and community-driven gene nomenclature for M. polymorpha. It includes keyword-searchable gene pages with structural and functional annotations, expression data integration, genome browser visualization, and online analytical and utility tools. By unifying genome assembly, annotation, nomenclature, and analysis tools in a single platform, MarpolBase serves as a central resource for functional genomics and evolutionary studies in M. polymorpha, and a model for future plant genome databases. The genomic resources of MarpolBase are freely available at https://marchantia.info.</p>","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"377-388"},"PeriodicalIF":4.0,"publicationDate":"2026-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13078153/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145649145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of fucoxanthin-deficient strains in the haptophyte Tisochrysis lutea induced by heavy-ion beam irradiation.","authors":"Shohei Kubo, Tomoharu Sano, Masanobu Kawachi, Yoshihiro Hase, Katsuya Satoh, Yutaka Oono, Yasushi Iwata, Hirotoshi Endo, Hiroya Araie, Kohei Yoneda, Yoshiaki Maeda, Iwane Suzuki","doi":"10.1093/pcp/pcaf149","DOIUrl":"10.1093/pcp/pcaf149","url":null,"abstract":"<p><p>Although microalgae such as diatoms and haptophytes have been studied to optimize fucoxanthin production, the complete biosynthetic pathway of fucoxanthin remains unclear. In this study, we subjected the haptophyte Tisochrysis lutea cells to heavy-ion beam irradiation to induce random mutations and obtained two greenish strains, GR1 and GR2, following exposures to 45 and 100 Gy, respectively. The GR1 strain exhibited slow growth, whereas GR2 showed growth comparable to the wild-type strain. Neither GR1 nor GR2 accumulated fucoxanthin; instead, both strains accumulated fucoxanthin biosynthetic intermediates, haptoxanthin and phaneroxanthin, and harbored 74 and 148 mutation sites, respectively. As expected, higher radiation doses resulted in a greater number of mutations. Over 80% of these mutations consisted of short nucleotide insertions, primarily 4-8 bp in length. Additionally, mutations were identified in orthologs of the zeaxanthin epoxidase 1 (ZEP1) and carotenoid isomerase 5 (CRTISO5) genes, known in the diatom Phaeodactylum tricornutum, to encode enzymes that convert haptoxanthin to phaneroxanthin and phaneroxanthin to fucoxanthin in GR1 and GR2 strains, respectively. The loss of fucoxanthin decreased photosynthetic capacity to some extent. However, the amounts of chlorophyll a and c did not change, suggesting that haptoxanthin and phaneroxanthin functioned as photosynthetic accessory pigments in the light-harvesting antennae. Because the genomic analysis results aligned with those from pigment analysis, our findings demonstrate that ZEP1 and CRTISO5 in T. lutea cells are involved in fucoxanthin biosynthesis and support the broader application of heavy-ion beam irradiation in fundamental microalgal research.</p>","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"265-274"},"PeriodicalIF":4.0,"publicationDate":"2026-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13078158/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145496424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Jasmonic and Salicylic Acid Pathways Shape the Rhizosphere Microbiome, affecting Aphid Herbivory and Soil-Mediated Insect-Plant Interactions in a density-dependent manner.","authors":"Marcela Aragón, Haris Spyridis, Pieter Mostard, Michael Reichelt, Jonathan Gershenzon, Marcel Dicke, Karen J Kloth","doi":"10.1093/pcp/pcag048","DOIUrl":"https://doi.org/10.1093/pcp/pcag048","url":null,"abstract":"<p><p>Aboveground induction of plant defense pathways can shape root-associated microbial communities. However, whether these changes are pathway-specific and how they affect plant growth and resistance remains unclear. We evaluated how induction of the Jasmonic Acid (JA) and Salicylic Acid (SA) defense pathways shapes the root microbiome of Brassica oleracea, and whether these soil-mediated shifts affect plant growth and resistance to herbivory in a subsequent generation using a plant-soil feedback (PSF) approach. In the conditioning phase, defense pathways were induced either through foliar application of methyl jasmonate (MeJA) and SA solutions, or through herbivory by caterpillars (JA) and aphids (SA). Both pathways led to distinct shifts in microbial communities, with bacterial and fungal composition varying by pathway identity and induction method. JA induction resulted in more differentially abundant ASVs than SA, particularly with Proteobacteria depletion. Conversely, Planctomycetota (bacteria) and Mortierellomycota (fungi) were enriched under both pathways, suggesting that these represent general stress-responsive groups. In the feedback phase, JA- and SA-conditioned soils had no effect on resistance under high aphid pressure, whereas under low aphid density, plants grown in SA-conditioned soil exhibited reduced phloem feeding and lower aphid population development. Together, our results indicate that benefits provided by the defense-shaped root microbiome depend on pest pressure intensity and arise from overall community shifts rather than specific taxa enrichment. Our findings underscore the complex interactions between plant-defense pathways, rhizosphere microbes, and herbivores.</p>","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147691818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heavy-ion beam mutagenesis uncovers fucoxanthin biosynthesis genes in haptophytes.","authors":"Sho Fujii","doi":"10.1093/pcp/pcag027","DOIUrl":"10.1093/pcp/pcag027","url":null,"abstract":"","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"228-231"},"PeriodicalIF":4.0,"publicationDate":"2026-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147284853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Community-building resources for Marchantia-an accessible model plant system.","authors":"William Boxall, Jim Haseloff","doi":"10.1093/pcp/pcag024","DOIUrl":"10.1093/pcp/pcag024","url":null,"abstract":"","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"225-227"},"PeriodicalIF":4.0,"publicationDate":"2026-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147618808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mining of key O-methyltransferase genes and UDP-glucosyltransferase genes for ginkgotoxin and ginkgotoxin-5'-glucoside synthesis based on transcriptome sequencing analysis.","authors":"Manman Shi, Wen Zhang, Tao Wang, Pengfei Yu, Fuliang Cao, Erzheng Su","doi":"10.1093/pcp/pcag046","DOIUrl":"https://doi.org/10.1093/pcp/pcag046","url":null,"abstract":"<p><p>Ginkgo biloba seeds contain toxic ginkgotoxin (MPN) and its glucoside (MPNG), but their biosynthetic genes are poorly understood. To identify these genes, we performed multi-stage transcriptome sequencing, DEG screening, WGCNA, and enrichment analysis during seed development. MPNG accumulation predominated in mature seeds, correlating with gene expression dynamics. We identified 25 O-methyltransferase (OMT) and 40 UDP-glucosyltransferase (UGT) candidates. Among these, seven OMTs (e.g., Gb_35680, Gb_01186) and eight UGTs (e.g., Gb_34745, Gb_37494) were significantly upregulated during maturation, confirmed by qRT-PCR. WGCNA revealed four gene modules strongly correlated with MPN/MPNG accumulation, enriched in methylation, glycosylation, and secondary metabolite biosynthesis. This study integrates dynamic transcriptome and co-expression analyses to pinpoint key regulatory nodes in ginkgo toxin biosynthesis, providing novel genetic targets for developing low-toxicity cultivars through molecular breeding.</p>","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147676012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}