Plant and Cell Physiology最新文献

{"title":"Generation of KARRIKIN INSENSITIVE2 loss-of-function mutants in Ceratopteris richardii using a CRISPR/Cas9 system based on ribozyme-gRNA-ribozyme (RGR) technology.","authors":"Ayano Wu, Seto Yoshiya, Junko Kyozuka, Yuki Hata","doi":"10.1093/pcp/pcag129","DOIUrl":"https://doi.org/10.1093/pcp/pcag129","url":null,"abstract":"","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148963599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Higher-Order Mutants of Conserved Kinases Expose a Cryptic Target of Flowering Regulation.","authors":"Mariko Asaoka","doi":"10.1093/pcp/pcag127","DOIUrl":"https://doi.org/10.1093/pcp/pcag127","url":null,"abstract":"","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148963575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highlights from the 14th International Conference for Plant Mitochondrial Biology: Current Trends and Future Directions.","authors":"Jingxiu Ji, Rika Nakajima, Nanami Kosaka, Hsu Yi Mon, Bernard Gutmann, Tomohiko Kazama, Mizuki Takenaka, Shin-Ichi Arimura, Wataru Sakamoto","doi":"10.1093/pcp/pcag125","DOIUrl":"https://doi.org/10.1093/pcp/pcag125","url":null,"abstract":"<p><p>Plant mitochondrial biology is undergoing a rapid transformation driven by advances in genomics, structural biology, quantitative imaging, and genome engineering. Once focused primarily on respiration and bioenergetics, the field now encompasses diverse areas including genome evolution, gene expression, organelle dynamics, stress signaling, metabolism, and biotechnology. The 14th International Conference for Plant Mitochondrial Biology (ICPMB), held in Kagoshima, Japan, from 18-22 May 2026 (Fig. 1), brought together researchers to discuss recent advances across these rapidly expanding research areas. This meeting report summarizes the major scientific advances presented at ICPMB 2026 and highlights emerging directions that are defining the future of plant mitochondrial biology.</p>","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148956141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arrest and tolerance: two modules of MpHYPNOS-dependent gemma dormancy in Marchantia.","authors":"Hatsune Morinaka","doi":"10.1093/pcp/pcag109","DOIUrl":"10.1093/pcp/pcag109","url":null,"abstract":"","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"1253-1255"},"PeriodicalIF":4.7,"publicationDate":"2026-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148631129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Involvement of tRNA thiolation in uORF-mediated translational regulation during Xylogenesis in Arabidopsis thaliana.","authors":"Yuichi Nishii, Daichi Araki, Mitsuru Saraumi, Taku Takahashi","doi":"10.1093/pcp/pcag055","DOIUrl":"10.1093/pcp/pcag055","url":null,"abstract":"<p><p>Post-transcriptional modification of tRNAs is an important mechanism for regulating translation efficiency and cellular homeostasis, yet its contribution to upstream open reading frame (uORF)-mediated translational control remains largely unexplored. In this study, we investigated the role of tRNA thiolation in thermospermine-dependent regulation of xylem development in Arabidopsis thaliana. Using a suppressor screen of the thermospermine-deficient mutant acaulis5 (acl5), which exhibits dwarfism and excessive xylem differentiation, we identified suppressor-of-acl502 (sac502) as a recessive loss-of-function allele of CTU2, a gene encoding a key enzyme in the biosynthesis of the wobble uridine modification 5-methoxycarbonylmethyl-2-thiouridine. Mutations in other components of the same modification pathway, including ROL5 and TRM9, similarly suppressed the acl5 phenotype. Translational analyses using 5' leader-GUS reporter constructs revealed that the ctu2 mutation did not enhance translation of the mRNA containing a thermospermine-responsive uORF of SAC51, but instead significantly reduced translation of that of SACL3, a member of the SAC51 family, and that of LONESOME HIGHWAY (LHW), which contains another conserved uORF in the 5' leader region. Polysome profiling further demonstrated decreased association of SACL3 and LHW mRNAs with actively translating ribosomes in ctu2. Genetic interaction analyses supported the conclusion that the suppression of excessive xylem formation in acl5 by ctu2 is attributable to reduced LHW activity. In addition, ctu2 mutants displayed increased sensitivity to exogenous thermospermine, resembling the response of lhw mutants. Together, our results reveal that tRNA thiolation contributes to uORF-mediated translational regulation of key developmental regulators and identify tRNA modification as an important regulatory layer controlling vascular development.</p>","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"1346-1355"},"PeriodicalIF":4.7,"publicationDate":"2026-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13545651/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147779427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trimming galactose side chains of arabinogalactan proteins alters pectin and hemicellulose deposition in secondary cell walls of Arabidopsis thaliana floral stem internodes.","authors":"Lucía Albornos, Paula Iriondo-Ocampo, Iván Campos-Seoane, Berta Dopico, Ignacio Martín","doi":"10.1093/pcp/pcag060","DOIUrl":"10.1093/pcp/pcag060","url":null,"abstract":"<p><p>Shaping the cell wall composition and structure to meet the requirements of different tissues and developmental stages relies on multiple actors, including arabinogalactan proteins (AGPs). Although the specific role of these proteins in cell wall dynamics is still under debate, especially in events involving significant remodeling of the cell wall, their carbohydrate motif, type II arabinogalactan (AGII), seems to be crucial for their function. This study aims to investigate the function of AGII, specifically the galactose residues of its side chains, in the structural organization of the cell wall during the cessation of elongation and the transition to secondary growth. To achieve this, we characterized floral stem internodes of Arabidopsis thaliana plants overproducing the chickpea βV-galactosidase protein (35S::βV-Gal plants), an enzyme that specifically hydrolyzes the β-(1,3)- and β-(1,6)-galactosyl residues of AGII. Changes induced in the cell wall by trimming galactose residues of AGII resulted in a noticeable increase in homogalacturonan methyl esterification. Additionally, these neutral galactose side chains may regulate hemicellulose-cellulose interactions and influence xylan distribution through the cellulose network, which in turn affects the deposition of lignin and determines its recalcitrance to enzymatic degradation.</p>","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"1385-1398"},"PeriodicalIF":4.7,"publicationDate":"2026-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13545650/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147842087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Purine permease 5 contributes to riboflavin distribution in Arabidopsis reproductive organs.","authors":"Rui Shibata, Hikari Kuwata, Takuto Sugimoto, Madoka Kikuchi, Takahiro Ishikawa, Takahisa Ogawa","doi":"10.1093/pcp/pcag061","DOIUrl":"10.1093/pcp/pcag061","url":null,"abstract":"<p><p>Riboflavin (vitamin B₂; RF) and its derivatives flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) are indispensable cofactors for redox reactions in plants. While higher plants possess a conserved pathway for de novo RF biosynthesis, how flavins are transported and spatially distributed between tissues remains unresolved. In particular, the molecular identity of plasma membrane (PM) transporters involved in flavin transport in plants remains largely unknown. Here, we identify Arabidopsis purine permease 5 (AtPUP5) as a PM-localized protein associated with RF transport and its distribution in plants. Using an RF-auxotrophic yeast mutant, we show that AtPUP5 enhances intracellular accumulation of RF and, to a lesser extent, FMN, whereas FAD accumulation is inefficient under the conditions tested. In planta, AtPUP5 overexpression increases RF accumulation following external application. In contrast, loss-of-function mutants do not display defects in bulk RF uptake at the whole-plant level, indicating that AtPUP5 is not essential for global RF acquisition. Notably, AtPUP5 deficiency results in RF overaccumulation in reproductive organs, including inflorescences, siliques, and seeds, irrespective of external RF supply. This organ-specific phenotype is fully suppressed by genetic complementation and coincides spatially with strong AtPUP5 promoter activity in reproductive tissues. These findings suggest that AtPUP5 contributes to the regulation of localized RF distribution in reproductive tissues. Together, our study provides a molecular framework for PM-mediated regulation of RF distribution in plants and highlights spatial regulation as an important component of flavin homeostasis.</p>","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"1399-1413"},"PeriodicalIF":4.7,"publicationDate":"2026-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13545647/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147942642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A SABATH family enzyme regulates development in part via the gibberellin-related pathway in the liverwort Marchantia polymorpha.","authors":"Shogo Kawamura, Eita Shimokawa, Maika Ito, Isuzu Nakamura, Takehiko Kanazawa, Megumi Iwano, Rui Sun, Yoshihiro Yoshitake, Shohei Yamaoka, Shinjiro Yamaguchi, Takashi Ueda, Misako Kato, Takayuki Kohchi","doi":"10.1093/pcp/pcag039","DOIUrl":"10.1093/pcp/pcag039","url":null,"abstract":"<p><p>The SABATH family enzymes are a group of plant-specific methyltransferases that catalyze the methylation of many small molecules, including several plant hormones. While this family originated before the evolution of land plants from streptophyte algae, little is known about its biological function in plant lineages other than angiosperms. Here, we identified 12 SABATH family genes from the liverwort Marchantia polymorpha and found that MpSABATH2 plays a critical role in development. Mpsabath2 mutants were severely inhibited in thallus growth and gemma cup formation, while they spontaneously formed sexual branches under noninductive conditions. These phenotypes resembled the developmental responses to far-red light, which was also supported by transcriptome analysis. Further genetic analysis connected this phenomenon with gibberellin (GA)-related metabolism. Blocking GA biosynthesis partially rescued Mpsabath2 phenotypes, which were restored by treatment with the GA precursor, ent-kaurenoic acid. Given that MpSABATH2 belongs to a phylogenetic clade distinct from previously reported phytohormone-methylating SABATH enzymes, our findings suggest that SABATH family enzymes independently acquired roles in developmental regulation through parallel evolution in land plants.</p>","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"1316-1327"},"PeriodicalIF":4.7,"publicationDate":"2026-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147504467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pinoresinol/lariciresinol reductases and secoisolariciresinol dehydrogenases involved in the specific production of (+)-enantiomer of matairesinol in Daphne genkwa.","authors":"Fernando Satoshi Tutihashi, Mitsuki Hirota, Masaomi Yamamura, Keisuke Kobayashi, Takefumi Hattori, Yuki Tobimatsu, Björn Robert Hamberger, Toshiaki Umezawa","doi":"10.1093/pcp/pcag054","DOIUrl":"10.1093/pcp/pcag054","url":null,"abstract":"<p><p>Lignans are phenylpropanoid dimers in which the monomers are linked by the central carbon atoms of their propyl side chains. In addition to the biosynthesis of biologically active lignans, the unique stereochemical mechanisms of biosynthetic reactions have been of long-standing interest. The enantiomeric composition of pinoresinol and lariciresinol-the two lignans farthest upstream in the lignan biosynthetic pathway-varies greatly among the plant species; these compounds are not enantiomerically pure and are mixtures of both enantiomers. The enantiomeric composition of the next intermediate, secoisolariciresinol, also varies by plant species, with some being enantiomerically pure. Conversely, the next compound, matairesinol, is always enantiomerically pure. In most cases, it is levorotatory, while the opposite dextrorotatory enantiomer, (+)-matairesinol, has been obtained from Thymelaeaceae plants, including Daphne genkwa. The conversion of pinoresinol to secoisolariciresinol via lariciresinol is catalyzed by pinoresinol/lariciresinol reductases (PLRs). Secoisolariciresinol is then oxidized by secoisolariciresinol dehydrogenases (SIRDs) to afford matairesinol. Many PLRs from various plant species preferentially reduce (+)-pinoresinol to (-)-secoisolariciresinol, while others preferentially reduce (-)-pinoresinol to (+)-secoisolariciresinol. However, the specific formation and accumulation of (+)-matairesinol in Thymelaeaceae plants remained unknown. This study demonstrates that DgPLRs selectively reduce (-)-lariciresinol but not (+)-lariciresinol, resulting in the formation of (+)-secoisolariciresinol. Furthermore, DgSIRDs were found to selectively oxidize (+)-secoisolariciresinol over (-)-secoisolariciresinol, yielding (+)-matairesinol. Thus, the selectivity of DgPLRs for lariciresinol enantiomers, rather than pinoresinol enantiomers and the selectivity of DgSIRDs for secoisolariciresinol enantiomers, are responsible for the accumulation of nearly enantiomerically pure (+)-secoisolariciresinol and enantiomerically pure (+)-matairesinol in D. genkwa.</p>","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"1328-1345"},"PeriodicalIF":4.7,"publicationDate":"2026-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147779353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Triacylglycerol synthesis in microalgae under abiotic stress: an updated integrative review.","authors":"Vipul Swarup Bhatnagar, Marta Saldat, Agnieszka Zienkiewicz, Zhi-Yan Du, Krzysztof Zienkiewicz","doi":"10.1093/pcp/pcag059","DOIUrl":"10.1093/pcp/pcag059","url":null,"abstract":"<p><p>Microalgae exhibit remarkable metabolic plasticity, rapidly redirecting carbon toward triacylglycerol (TAG) accumulation under environmental stress. In this review, we synthesize recent advances in understanding how nutrient limitation, light fluctuations, salinity, temperature extremes, and chemical modulators regulate TAG biosynthesis in diverse microalgal systems. Under stress, TAG accumulation is typically linked to the coordinated transcriptional and metabolic reprogramming of the glycerol-3-phosphate (Kennedy) pathway, with diacylglycerol acyltransferases acting as the pivotal enzymes that complete TAG biosynthesis. However, these responses are highly species- and isoform-specific, reflecting substantial evolutionary divergence. In addition to enzyme-level control, increasing evidence points to a central role for intracellular signaling networks and transcription factors in coordinating these responses. Although upstream regulatory mechanisms differ, their outcomes commonly result in enhanced TAG accumulation and the remodeling of fatty acid pools. Together, these findings support a hierarchical model in which environmental sensing, signal integration, and transcriptional regulation coordinate TAG biosynthesis as an adaptive response to environmental stressors.</p>","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"1263-1286"},"PeriodicalIF":4.7,"publicationDate":"2026-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13545648/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147842084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}