Plant and Cell Physiology最新文献_第4页

The Circadian Morning Complex Modulates ABA Signaling by Transcriptionally Repressing PYL4, PYL5 and PYL6 at Dawn. 通过在黎明时转录抑制PYL4、PYL5和PYL6，昼夜节律清晨复合体调节ABA信号。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2026-04-10 DOI: 10.1093/pcp/pcag044

Chen Su, Yufan Niu, Yingjun Yu, Yan Wang, Yuqing He, Lei Wang

{"title":"The Circadian Morning Complex Modulates ABA Signaling by Transcriptionally Repressing PYL4, PYL5 and PYL6 at Dawn.","authors":"Chen Su, Yufan Niu, Yingjun Yu, Yan Wang, Yuqing He, Lei Wang","doi":"10.1093/pcp/pcag044","DOIUrl":"https://doi.org/10.1093/pcp/pcag044","url":null,"abstract":"The circadian clock not only coordinates plant growth with daily environmental changes but also interacts with endogenous hormonal pathways, such as abscisic acid (ABA) signaling, to balance growth and abiotic stress responses. Although the feedback network between the circadian system and ABA pathway has been well-established, the regulatory relationship between the ABA receptor PYRABACTIN RESISTANCE 1-LIKE (PYLs) family and the clock remains largely unclear, and the understanding of how ABA signaling feeds back to modulate the clock is still limited. Here, we show that the null mutants of TIME FOR COFFEE (TIC) and CIRCADIAN CLOCK ASSOCIATED 1/ LATE ELONGATED HYPOCOTYL (CCA1/LHY), which are core components of circadian Morning Complex (MC), display hypersensitivity to ABA during seed germination. Mechanistically, the MC could directly bind to the promoters of PYL4, PYL5 and PYL6 to repress their transcription at dawn, which may contribute to the attenuation of ABA signal transduction. Furthermore, ABA could induce TIC expression, and TIC is required for transferring ABA signals into coherent circadian rhythm. Taken together, these findings suggest that the MC may function as a dawn-specific integrator, linking ABA signaling with the circadian core oscillator, hence offering new insights into how plants coordinate hormonal and temporal regulation.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147676058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytochrome-Mediated Light Perception in Dodders Drives Haustorium Development Through Epigenetic Mechanisms. 菟丝子光敏色素介导的光感知通过表观遗传机制驱动吸器发育。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2026-04-02 DOI: 10.1093/pcp/pcag043

Thomas Bawin, Andreas Evenstad, Alena Didriksen, German Martinez, Kirsten Krause

{"title":"Phytochrome-Mediated Light Perception in Dodders Drives Haustorium Development Through Epigenetic Mechanisms.","authors":"Thomas Bawin, Andreas Evenstad, Alena Didriksen, German Martinez, Kirsten Krause","doi":"10.1093/pcp/pcag043","DOIUrl":"https://doi.org/10.1093/pcp/pcag043","url":null,"abstract":"Cuscuta species, parasitic plants with minimal photosynthetic capacity, rely on light cues to locate hosts and initiate infection. Unlike non-parasitic plants, they exhibit a reversed shade response, growing toward low red:far-red (R:FR) light typical of dense vegetation. We investigated how red and far-red light modulate haustorium development, gene expression, and epigenetic reprogramming in C. campestris. Far-red enriched conditions promoted coiling and haustorium initiation, while red light suppressed parasitic behaviour. Phytochromes B1 and B2 displayed opposing transcriptional responses to light quality, suggesting a modified light perception mechanism. Transcriptome analyses revealed further that far-red light triggered the differential expression of over 5,000 genes, including those linked to auxin and cytokinin signalling, cell wall remodelling, and organogenesis. Gene co-expression networks identified phytochrome B2 and a Fhy1/Fhl regulator of phytochrome A as possible central hubs associated with chromatin remodelers, histone modifiers, and RNA-directed DNA methylation components. Small RNA profiling indicated stable global sRNA populations across treatments, with shifts in the expression of specific miRNA families, affecting a subset of light-responsive genes. Our findings demonstrate that far-red light perception in C. campestris engages both transcriptional and epigenetic regulation to drive haustorium development, reflecting adaptations in light signalling cascades that underpin its parasitic lifestyle.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147594029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editing strigolactone biosynthesis genes in tomato reveals novel phenotypic effects and highlights D27 as a breeding target for parasitic weed resistance. 编辑番茄独角甾内酯生物合成基因揭示了新的表型效应，并突出了D27作为抗寄生杂草的育种靶点。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2026-04-01 DOI: 10.1093/pcp/pcag042

Alessandro Nicolia, Alessia Cuccurullo, Kento Tamada, Kaori Yoneyama, José Luis Rambla, Antonio Granell, Francesco Camerlengo, Giovanna Festa, Gianluca Francese, Felice Contaldi, Antonietta D'Alessandro, Maria Manuela Rigano, Luigia Principio, Nunzio D'Agostino, Teodoro Cardi

{"title":"Editing strigolactone biosynthesis genes in tomato reveals novel phenotypic effects and highlights D27 as a breeding target for parasitic weed resistance.","authors":"Alessandro Nicolia, Alessia Cuccurullo, Kento Tamada, Kaori Yoneyama, José Luis Rambla, Antonio Granell, Francesco Camerlengo, Giovanna Festa, Gianluca Francese, Felice Contaldi, Antonietta D'Alessandro, Maria Manuela Rigano, Luigia Principio, Nunzio D'Agostino, Teodoro Cardi","doi":"10.1093/pcp/pcag042","DOIUrl":"https://doi.org/10.1093/pcp/pcag042","url":null,"abstract":"Parasitic weed infestations represent an increasing threat to agriculture worldwide, especially in the Mediterranean region. Phelipanche ramosa (L.) and P. aegyptiaca (Pers.) (broomrapes) cause severe yield losses in field-grown tomato (Solanum lycopersicum L.). Strigolactones (SLs) are apocarotenoid phytohormones that play a critical role in plant physiology and development but also act as the primary germination signals for parasitic weed seeds. In this study, we generated CRISPR/Cas9 tomato knock-out (KO) lines targeting the SlD27 gene and three other key genes involved in SL biosynthesis (SlCCD7, SlCCD8, SlMAX1), all in the same genetic background. All edited lines exhibited undetectable SL levels in root exudates, leading to a strong reduction in the in vitro germination of Phelipanche spp. seeds. Consistently, reduced parasitism was also observed in vivo when Sld27 lines were tested. A comprehensive evaluation of morphological, reproductive, and fruit-related traits revealed gene-specific phenotypic effects, including changes in vegetative growth, fruit set, fruit development, and volatilome. Specifically, knock-out of two CCDs and the SlMAX1 affected shoot architecture, fruit development, and the production of volatile organic compounds during fruit ripening. In contrast, the Sld27 lines, newly developed in this study, displayed a mild phenotype generally comparable to non-edited control plants and likely due to the expression of SlD27 paralogues. Overall, our results indicate that SlD27 represents a promising breeding target for enhancing resistance to parasitic weeds in tomato while minimizing negative impacts on plant development and fruit quality.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147594005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The transcription factor WRKY13 activates BRUTUS to maintain iron homeostasis in Arabidopsis. 转录因子WRKY13激活BRUTUS以维持拟南芥中的铁稳态。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2026-03-31 DOI: 10.1093/pcp/pcag041

Hui Song, Yifan Chen, Guangna Chen, Shuangyao Li, Shuxun Xu, Jing Zhang, Xi Wu, Shuqing Cao

{"title":"The transcription factor WRKY13 activates BRUTUS to maintain iron homeostasis in Arabidopsis.","authors":"Hui Song, Yifan Chen, Guangna Chen, Shuangyao Li, Shuxun Xu, Jing Zhang, Xi Wu, Shuqing Cao","doi":"10.1093/pcp/pcag041","DOIUrl":"https://doi.org/10.1093/pcp/pcag041","url":null,"abstract":"Iron (Fe) is a fundamental micronutrient for plant and animal physiology. Consequently, Fe limitation in agricultural systems not only compromises crop productivity but also poses a significant threat to human nutritional security. Due to the limited bioavailability of Fe in the soil, plants have evolved adaptive strategies that can regulate Fe absorption and internal balance, thereby optimizing their survival ability under Fe-deficient conditions. BTS (BRUTUS), a Fe sensor possessing E3 ligase activity, acts as a negative regulator of the Fe deficiency response by targeting bHLH subgroup IVc transcription factors for degradation. In this study, we employed yeast one-hybrid screening to identify WRKY13, an Arabidopsis transcription factor that directly regulates BTS transcription. The expression patterns of WRKY13 and BTS exhibited similarity during the transition from Fe deficiency to Fe-resupply. WRKY13 mutants display increased tolerance to Fe deficiency, whereas WRKY13 complementation lines reverse the tolerance of wrky13 mutants to wild-type levels, effectively reversing the mutant phenotype under Fe-deficient conditions. Furthermore, WRKY13 was found to directly activate BTS expression by binding to W-box motifs within the BTS promoter region. Genetic analysis positioned WRKY13 upstream of BTS within the Fe homeostasis pathway. Collectively, our findings indicate that Fe deficiency stress induces WRKY13 expression, which in turn directly activates BTS transcription to maintain cellular Fe homeostasis. In addition, upon Fe resupply, WRKY13 potentiates BTS transcriptional activation, thereby fine-tuning Fe uptake and preventing potential toxicity caused by Fe overload.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147581500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Synteny Conserved TATA Binding Protein 2-associated Long Intergenic Non-coding RNA Influences Leaf Development and ABA Response in Arabidopsis thaliana. TATA结合蛋白2相关的长基因间非编码RNA影响拟南芥叶片发育和ABA响应。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2026-03-26 DOI: 10.1093/pcp/pcag040

Byanka Sthefany Espinoza-López, Jesús Nieto-Hernández, Ángel Eduardo Quezada Eguía, Susana Isabel Vargas Camacho, Jaime Aportela Cortez, José Luis Reyes, Catalina Arenas-Huertero

{"title":"A Synteny Conserved TATA Binding Protein 2-associated Long Intergenic Non-coding RNA Influences Leaf Development and ABA Response in Arabidopsis thaliana.","authors":"Byanka Sthefany Espinoza-López, Jesús Nieto-Hernández, Ángel Eduardo Quezada Eguía, Susana Isabel Vargas Camacho, Jaime Aportela Cortez, José Luis Reyes, Catalina Arenas-Huertero","doi":"10.1093/pcp/pcag040","DOIUrl":"https://doi.org/10.1093/pcp/pcag040","url":null,"abstract":"Long non-coding RNAs are molecules with regulatory potential involved in many biological processes of plants. Despite their significant role, only a limited number have been studied in detail. Here, we describe at the molecular and bioinformatic levels a TBP2-associated long intergenic non-coding RNA (TALIR) encoded upstream of TATA-binding protein 2 (TBP2) of Arabidopsis thaliana. Downregulation of TALIR leads to plants with reduced leaf and rosette size, as well as decreased lateral root density. The expression of TBP2 in talir mutant lines is increased, which affects the timing of expression of MIR396 and GRF2, as well as MIR156 and SPL9. Therefore, these dysregulations may, in part, contribute to the altered leaf size observed. Transcriptional evidence from TBP2 loci suggests the presence of a putative TALIR ortholog gene conserved across the Brassiceae, Eutremeae, and Camelineae tribes at the DNA sequence and synteny level. Furthermore, TALIR is upregulated upon ABA and NaCl treatments in WT plants, whereas talir mutants exhibit insensitivity to ABA during germination. Together, these findings expand current knowledge of lncRNAs and provide new insights into the regulation of MIR396 and MIR156 in the control of leaf growth. Further, the evolutionary conservation of TALIR among tribes within the Brassicaceae family suggests a conserved functional role in this group of plants.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147513892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A SABATH family enzyme regulates development in part via the gibberellin-related pathway in the liverwort Marchantia polymorpha. SABATH家族酶在一定程度上通过赤霉素相关途径调节多形地茅的发育。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2026-03-24 DOI: 10.1093/pcp/pcag039

Shogo Kawamura, Eita Shimokawa, Maika Ito, Isuzu Nakamura, Takehiko Kanazawa, Megumi Iwano, Rui Sun, Yoshihiro Yoshitake, Shohei Yamaoka, Shinjiro Yamaguchi, Takashi Ueda, Misako Kato, Takayuki Kohchi

{"title":"A SABATH family enzyme regulates development in part via the gibberellin-related pathway in the liverwort Marchantia polymorpha.","authors":"Shogo Kawamura, Eita Shimokawa, Maika Ito, Isuzu Nakamura, Takehiko Kanazawa, Megumi Iwano, Rui Sun, Yoshihiro Yoshitake, Shohei Yamaoka, Shinjiro Yamaguchi, Takashi Ueda, Misako Kato, Takayuki Kohchi","doi":"10.1093/pcp/pcag039","DOIUrl":"https://doi.org/10.1093/pcp/pcag039","url":null,"abstract":"The SABATH family enzymes are a group of plant-specific methyltransferases that catalyze the methylation of many small molecules, including several plant hormones. While this family emerged anciently before the evolution of land plants from streptophyte algae, little is known about their biological function in plant lineages other than angiosperms. Here, we identified 12 SABATH family genes from the liverwort Marchantia polymorpha and found that MpSABATH2 plays a critical role in development. Mpsabath2 mutants were severely inhibited in thallus growth and gemma cup formation, while spontaneously forming sexual branches under non-inductive conditions. These phenotypes resembled the developmental responses to far-red light, which was also supported by transcriptome analysis. Further genetic analysis connected this phenomenon with gibberellin (GA)-related metabolism. Blocking GA biosynthesis partially rescued Mpsabath2 phenotypes, which were restored by treatment with the GA precursor, ent-kaurenoic acid. Given that MpSABATH2 belongs to a phylogenetic clade distinct from previously reported phytohormone-methylating SABATH enzymes, our findings suggest that SABATH family enzymes independently acquired roles in developmental regulation through parallel evolution in land plants.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147504467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic analyses reveal common heterophylly regulations in eight amphibious eudicots. 转录组学分析揭示了八种两栖植物的共同异源性调控。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2026-03-23 DOI: 10.1093/pcp/pcag037

Gaojie Li, Tomoaki Sakamoto, Seisuke Kimura, Hongwei Hou

{"title":"Transcriptomic analyses reveal common heterophylly regulations in eight amphibious eudicots.","authors":"Gaojie Li, Tomoaki Sakamoto, Seisuke Kimura, Hongwei Hou","doi":"10.1093/pcp/pcag037","DOIUrl":"https://doi.org/10.1093/pcp/pcag037","url":null,"abstract":"Heterophylly, the ability of plants to produce various leaf forms in response to environments, is widely seen in nature. Aquatic plants emerged from various lineages of land plants, and amphibious species are those that can survive in both terrestrial and submerged conditions. In this study, we conducted phenotypical studies and transcriptomic analyses of eight amphibious eudicots. We quantified leaf shape changes under terrestrial and submerged conditions and found that dissection index (DI) is a proper indicator to quantify leaf complexity among heterophyllous species. We identified groups of differentially expressed genes (DEGs) related to heterophylly, mainly in terms of leaf shape, stomata development, and phytohormone biosynthesis. Different heterophyllous species have diverse regulations of leaf shape and we verified the function of candidate class I KNOTTED1-LIKE HOMEOBOX (KNOX1) genes using transgenic Arabidopsis lines. Stomatal densities are commonly decreased under water and genes related to its development are also downregulated. Abscisic acid (ABA) may be a key factor regulating heterophylly, and CATALASE genes are upregulated under submergence. Our study uncovered common and special characteristics among eight amphibious eudicots, providing resources to reveal mechanisms of heterophylly and convergent evolutions.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147504527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Actin fragmentation induced by Colletotrichum higginsianum infection facilitates hyphal invasion in Arabidopsis thaliana leaves. 炭疽杆菌侵染诱导的肌动蛋白断裂有利于菌丝侵入拟南芥叶片。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2026-03-23 DOI: 10.1093/pcp/pcag038

Takashi L Shimada, Takumi Higaki, Kazuo Ebine, Yoshitaka Takano, Yusuke Saijo, Takashi Ueda

{"title":"Actin fragmentation induced by Colletotrichum higginsianum infection facilitates hyphal invasion in Arabidopsis thaliana leaves.","authors":"Takashi L Shimada, Takumi Higaki, Kazuo Ebine, Yoshitaka Takano, Yusuke Saijo, Takashi Ueda","doi":"10.1093/pcp/pcag038","DOIUrl":"https://doi.org/10.1093/pcp/pcag038","url":null,"abstract":"Actin filaments are essential for plant cellular functions, and their dynamics are altered in response to fungal infection, contributing to defense responses. However, the mechanisms by which pathogens suppress actin-mediated immunity remain largely unknown. Here, we report that infection by the adapted fungus Colletotrichum higginsianum induces actin fragmentation in Arabidopsis thaliana leaf cells. Imaging analyses revealed that actin fragmentation with increased apparent actin thickness occurred following C. higginsianum inoculation. Actin fragmentation was observed two days after inoculation, preceding hyphal invasion on day three. Rhodamine-phalloidin staining confirmed that endogenous actin filaments were disrupted in infected cells. Pretreatment with latrunculin B, which induces actin fragmentation, significantly increased the rate of C. higginsianum hyphal invasion, suggesting that fragmented actin filaments may facilitate fungal entry. Notably, actin fragmentation occurred even in pattern recognition receptor mutants, indicating that it is not triggered by pathogen-associated molecular patterns (PAMP) perception. No obvious changes in microtubule organization were observed. Inoculation with the non-adapted C. tropicale did not induce actin fragmentation in A. thaliana. In contrast, C. higginsianum induced actin fragmentation in cucumber and Brassica crops, whereas C. orbiculare did not, despite being pathogenic to cucumber. These findings suggest that C. higginsianum employs a distinctive infection strategy involving actin fragmentation that is independent of canonical immune recognition.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147499691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

2b proteins of highly virulent cucumber mosaic virus strains are associated with suppression of DCL4 activity. 高毒黄瓜花叶病毒株的2b蛋白与DCL4活性的抑制有关。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2026-03-23 DOI: 10.1093/pcp/pcag036

Toshiyuki Fukuhara, Shuhei Miyashita, Nobuhiro Suzuki, Hideki Takahashi

{"title":"2b proteins of highly virulent cucumber mosaic virus strains are associated with suppression of DCL4 activity.","authors":"Toshiyuki Fukuhara, Shuhei Miyashita, Nobuhiro Suzuki, Hideki Takahashi","doi":"10.1093/pcp/pcag036","DOIUrl":"https://doi.org/10.1093/pcp/pcag036","url":null,"abstract":"RNA silencing constitutes a fundamental antiviral defense mechanism in plants. Although its role in virus resistance has been extensively studied, the relationship between the dicing activity of Dicer-an essential enzyme in RNA silencing-and antiviral defense remains unclear. To address this knowledge gap, we previously established a simple in vitro dicing assay that enables the direct assessment of the activities of two Dicers, DCL3 and DCL4. We hypothesized that this assay could reveal how viral infection modulates DCL3 and DCL4 activities. Here, we report a negative correlation between NbDCL4 activity and symptom severity using the Nicotiana benthamiana-cucumber mosaic virus (CMV) system. We demonstrate that NbDCL4 activity is specifically suppressed in plants infected with highly virulent subgroup I (sg I) strains of CMV. In vitro dicing assays and small RNA sequencing analyses further reveal that NbDCL4 activity toward long double-stranded RNA (dsRNA) remains unaffected, whereas its activity toward shorter dsRNAs (~50 nt) is markedly inhibited in CMV(sg I)-infected plants. Subsequent analyses using a 2b-deficient disarmed mutant and a less virulent subgroup II strain of CMV indicate that this inhibition is mediated by the sg I 2b protein, a viral suppressor of RNA silencing. Taken together, these findings provide mechanistic insights into the molecular basis of CMV virulence, suggesting that the severe symptoms induced by highly virulent CMV strains are, at least in part, attributable to 2b-mediated inhibition of DCL4 activity.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147499697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional insights into a putative 2-OG oxygenase involved in jasmonic acid metabolism and xylem development in Populus trichocarpa. 2-OG加氧酶参与毛杨茉莉酸代谢和木质部发育的功能研究。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2026-03-16 DOI: 10.1093/pcp/pcag035

Natalia Wojciechowska, Maciej Janicki, Katarzyna Marzec-Schmidt, Thierry Heitz, Magdalena Ślachetka, Agnieszka Ludwików, Agnieszka Bagniewska-Zadworna

{"title":"Functional insights into a putative 2-OG oxygenase involved in jasmonic acid metabolism and xylem development in Populus trichocarpa.","authors":"Natalia Wojciechowska, Maciej Janicki, Katarzyna Marzec-Schmidt, Thierry Heitz, Magdalena Ślachetka, Agnieszka Ludwików, Agnieszka Bagniewska-Zadworna","doi":"10.1093/pcp/pcag035","DOIUrl":"https://doi.org/10.1093/pcp/pcag035","url":null,"abstract":"The xylem vascular tissue constitutes an efficient system of long-distance transport of water and nutrients, as well as structural support to the plant body. Despite its importance, the molecular mechanisms regulating xylogenesis remain only partially understood. In this study, we investigated two genes from Populus trichocarpa, encoding 2-oxoglutarate and Fe(II)-dependent oxygenases, whose expression is significantly upregulated during secondary growth. Proteins encoded by those genes share similarities with Arabidopsis enzymes known as jasmonic acid oxygenases (JAOs) or jasmonate-induced oxygenases (JOXs), which are involved in the hydroxylation of jasmonic acid (JA) - a hormone precursor critical for plant development and stress responses. We hypothesized that the Populus 2-OG oxygenases participate in JA metabolism and thereby influence wood formation. Their in silico structural analysis indicated that one of these proteins likely binds JA with similar efficiency to known JAO proteins. A link was supported by in vitro and in vivo experiments. Studies in an Arabidopsis JAO-depleted mutant revealed altered stem anatomy, characterized by a reduced stem diameter and an increased proportion of xylem area relative to the total stem cross-section, suggesting that disrupted JA metabolism can interfere with normal vascular development. These findings provide new insights into the hormonal regulation of vascular development and may have practical implications for improving wood quality or stress resilience in trees.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147463776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0