Plant and Cell Physiology最新文献_第4页

Probing Bilin-Protein Interaction in the Protochromic Photocycle of Cyanobacteriochrome RcaE by Site-Directed Mutagenesis. 通过定点突变探究蓝藻色素 RcaE 的原色光周期中的双蛋白-蛋白相互作用。

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-02-28 DOI: 10.1093/pcp/pcae085

Takanari Kamo, Takaaki Matsushita, Masako Hamada, Tomotsumi Fujisawa, Toshihiko Eki, Masashi Unno, Yuu Hirose

{"title":"Probing Bilin-Protein Interaction in the Protochromic Photocycle of Cyanobacteriochrome RcaE by Site-Directed Mutagenesis.","authors":"Takanari Kamo, Takaaki Matsushita, Masako Hamada, Tomotsumi Fujisawa, Toshihiko Eki, Masashi Unno, Yuu Hirose","doi":"10.1093/pcp/pcae085","DOIUrl":"10.1093/pcp/pcae085","url":null,"abstract":"Cyanobacteriochromes (CBCRs) are members of the phytochrome superfamily of photosensor proteins that bind a bilin chromophore. CBCRs exhibit substantial diversity in their absorption wavelengths through a variety of bilin-protein interactions. RcaE is the first discovered CBCR as a regulator of chromatic acclimation, where cyanobacteria optimize the absorption wavelength of their photosynthetic antenna. RcaE undergoes a reversible photoconversion between green-absorbing (Pg) and red-absorbing (Pr) states, where the bilin chromophore adopts a deprotonated C15-Z,anti and a protonated C15-E,syn structures, respectively. This photocycle is designated as the 'protochromic photocycle' as the change in the bilin protonation state is responsible for the large absorption shift. With the guidance of recently determined Pg and Pr structures of RcaE, in this study, we investigated bilin-protein interaction by site-directed mutagenesis on three key residues referred to as a protochromic triad and also other conserved residues interacting with the bilin. Among the protochromic triad residues, Glu217 and Lys261 are critical for the formation of the Pr state, while Leu249 is critical for the formation of both Pg and Pr states. Substitution in other conserved residues, including Val218, Phe219 and Pro220 in the wind-up helix and Phe252, Phe214 and Leu209 in a part of the bilin-binding pocket, had less substantial effects on the spectral sensitivity in RcaE. These data provide insights into our understanding of the bilin-protein interaction in the protochromic photocycle and also its evolution in the CBCRs.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"181-192"},"PeriodicalIF":3.9,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141875771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular Vesicle-Mediated Secretion of Chlorophyll Biosynthetic Intermediates in the Cyanobacterium Leptolyngbya boryana. 蓝藻 Leptolyngbya boryana 细胞外囊泡介导的叶绿素生物合成中间体分泌。

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-02-28 DOI: 10.1093/pcp/pcae095

Kentaro Usui, Haruki Yamamoto, Hitoshi Mori, Yuichi Fujita

{"title":"Extracellular Vesicle-Mediated Secretion of Chlorophyll Biosynthetic Intermediates in the Cyanobacterium Leptolyngbya boryana.","authors":"Kentaro Usui, Haruki Yamamoto, Hitoshi Mori, Yuichi Fujita","doi":"10.1093/pcp/pcae095","DOIUrl":"10.1093/pcp/pcae095","url":null,"abstract":"Extracellular vesicles (EVs) are derived from outer membranes (OMs) in Gram-negative bacteria and have diverse physiological functions. EV-mediated secretion of monovinyl protochlorophyllide (MV-Pchlide), the chlorophyll a (Chl) biosynthetic intermediate, was previously reported in a mutant lacking dark-operative Pchlide reductase in the cyanobacterium Leptolyngbya boryana. This study showed a detailed characterization of EVs from wild-type (WT) strain of L. boryana grown under photoautotrophic and dark heterotrophic conditions, focusing on the accumulation of Chl intermediates. WT L. boryana cells produce two types of EVs, low-density EVs (L-EVs) and high-density EVs (H-EVs), both under light and dark conditions. L-EVs and H-EVs showed distinct morphological features and protein compositions. L-EVs from cells grown under both light and dark conditions commonly contained carotenoids, ketomyxol glycoside and zeaxanthin as major pigments. Based on the protein compositions of EVs and other cellular membrane fractions, L-EVs and H-EVs are probably derived from low-density OMs and high-density OMs interacting with cell walls, respectively. Fluorescence detection of pigments was applied to EVs, and the two Chl intermediates, protoporphyrin IX and protoporphyrin IX monomethyl ester, were commonly detected in both L-EVs from light- and dark-grown cells, whereas L-EVs from dark-grown cells contained additional MV-Pchlide, MV-protopheophorbide and pheophorbide. The pigment ratios of L-EVs to the total culture medium of the Chl intermediates were much higher than those of carotenoids, suggesting an active transport of the Chl intermediates from the thylakoid membrane to L-EVs. Cyanobacterial EVs may play a novel role in alleviating the accumulation of Chl intermediates in cells.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"214-228"},"PeriodicalIF":3.9,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11879085/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142036758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and functional analysis of strigolactone pathway genes regulating tillering traits in sugarcane. 调节甘蔗分蘖性状的独角麦内酯途径基因的鉴定与功能分析。

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-02-28 DOI: 10.1093/pcp/pcae146

Yiying Qi, Xiaoxi Feng, Hongyan Ding, Dadong Lin, Yuhong Lan, Yixing Zhang, Sehrish Akbar, Huihong Shi, Zhen Li, Ruiting Gao, Xiuting Hua, Yuhao Wang, Jisen Zhang

{"title":"Identification and functional analysis of strigolactone pathway genes regulating tillering traits in sugarcane.","authors":"Yiying Qi, Xiaoxi Feng, Hongyan Ding, Dadong Lin, Yuhong Lan, Yixing Zhang, Sehrish Akbar, Huihong Shi, Zhen Li, Ruiting Gao, Xiuting Hua, Yuhao Wang, Jisen Zhang","doi":"10.1093/pcp/pcae146","DOIUrl":"10.1093/pcp/pcae146","url":null,"abstract":"Saccharum officinarum and Saccharum spontaneum are two fundamental species of modern sugarcane cultivars, exhibiting divergent tillering patterns crucial for sugarcane architecture and yield. Strigolactones (SLs), a class of plant hormones, are considered to play a central role in shaping plant form and regulating tillering. Our study highlights the distinct tillering patterns observed between S. officinarum and S. spontaneum and implicates significant differences in SL levels in root exudates between the two species. Treatment with rac-GR24 (an artificial SL analog) suppressed tillering in S. spontaneum. Based on transcriptome analysis, we focused on two genes, TRANSCRIPTION ELONGATION FACTOR 1 (TEF1) and CIRCADIAN CLOCK ASSOCIATED1 (CCA1), which show higher expression in S. spontaneum or S. officinarum, respectively. While the overexpression of SoCCA1 did not lead to significant phenotypic differences, overexpression of SsTEF1 in rice stimulated tillering and inhibited plant height, demonstrating its role in tillering regulation. However, the overexpression of suggests that SoCCA1 may not be the key regulator of sugarcane tillering. Yeast one-hybrid assays identified four transcription factors (TFs) regulating SsTEF1 and four and five TFs regulating SsCCA1 and SoCCA1. This study provides a theoretical foundation for deciphering the molecular mechanisms underlying the different tillering behaviors between S. officinarum and S. spontaneum, providing valuable insights for the molecular-based design of sugarcane breeding strategies.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"260-272"},"PeriodicalIF":3.9,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Substrate specificities in the chlorophyll catabolism of aquatic protists determined with in vivo phagocytotic assays. 通过体内吞噬试验确定水生原生动物叶绿素分解代谢的底物特异性。

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-02-28 DOI: 10.1093/pcp/pcae136

Yuichiro Kashiyama, Shizuka Miichi, Hiroki Imanishi, Moe Maruyama, Shin Ogasawara, Hitoshi Tamiaki

{"title":"Substrate specificities in the chlorophyll catabolism of aquatic protists determined with in vivo phagocytotic assays.","authors":"Yuichiro Kashiyama, Shizuka Miichi, Hiroki Imanishi, Moe Maruyama, Shin Ogasawara, Hitoshi Tamiaki","doi":"10.1093/pcp/pcae136","DOIUrl":"10.1093/pcp/pcae136","url":null,"abstract":"Chlorophylls (Chls) are ubiquitous photosynthetic pigments with inherent potential to generate cytotoxic reactive oxygen species. Therefore, all phototrophs and any phagotrophs that attempt to digest phototrophic cells have presumably developed mechanisms to mitigate this phototoxicity. In aquatic environments, the Chls produced by the dominant producers, microalgae, are catabolized into nonphototoxic pigments, cyclopheophorbide enols (CPEs), either by microalga-feeding protists or autonomously, particularly by those carrying secondary chloroplasts during the dismantling of their chloroplasts. However, the biochemistry underpinning CPE-accumulating Chl catabolism remains largely unexamined. To characterize the reactions in the transformation pathway and identify the pivotal enzyme for the formation of the seven-membered ring distinctive to CPEs, we conducted qualitative in vivo experiments using hemisynthetically prepared Chl derivatives in the cells of a euglenozoan algivorous (phycophagic) protist, Peranema trichophorum NIES-4660. We supplied polymer beads coated with Chl-b derivatives with their food cells, a unicellular red alga, Cyanidioschyzon merolae, which exclusively contains Chl-a. After administration of Chl-b or its free base with the beads, we detected a CPE derivative with a formyl group at the C7 position [cyclopheophorbide b-enol (cPPB-bE)], clearly derived from the appended derivatives, and not from the Chl-a of the alga. In contrast, cPPB-bE was not detected when zinc- and copper-metalated Chls and C132-demethoxycarbonylated Chl-b were added, although the latter resulted in the generation of its demetalated free-base form. These results indicate that (I) pheophytins are the actual substrates of the cyclization enzyme and (II) cyclization proceeds after the enzymatic dechelation of the central magnesium of natural Chls.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"238-248"},"PeriodicalIF":3.9,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and characterization of PpUGT91BP1 as a trillin synthase from Paris polyphylla. 鉴定并确定 PpUGT91BP1 是来自巴黎多花植物的三聚氰胺合成酶。

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-02-28 DOI: 10.1093/pcp/pcae147

Shuyu Li, Sa Chen, Chunjin Fu, Jingjing Zhang, Jiale Xing, Xin Chai, Qian Zhang, Jie Zhou, Haining Lyu, Chengchao Xu, Jingjing Liao

{"title":"Identification and characterization of PpUGT91BP1 as a trillin synthase from Paris polyphylla.","authors":"Shuyu Li, Sa Chen, Chunjin Fu, Jingjing Zhang, Jiale Xing, Xin Chai, Qian Zhang, Jie Zhou, Haining Lyu, Chengchao Xu, Jingjing Liao","doi":"10.1093/pcp/pcae147","DOIUrl":"10.1093/pcp/pcae147","url":null,"abstract":"Polyphyllins are the active ingredients of the medicinal plant Paris polyphylla. The biosynthesis of different types of polyphyllins all requires the catalysis of glycosyltransferases. Even though significant efforts have been made to identify PpUGTs capable of catalyzing the initial glycosylation reaction, the specific glycosyltransferases responsible for the synthesis of trillin have not been reported in P. polyphylla. Here, we identified a new trillin synthase, named PpUGT91BP1, which was highly expressed in the rhizome. Importantly, PpUGT91BP1 could specifically glycosylate diosgenin but not pennogenin. To improve its catalytic efficiency, we introduced random mutations through error-prone PCR and conducted an activity-based screening. Three mutants with significantly enhanced trillin synthase activity were identified. Finally, we successfully reconstituted trillin biosynthesis in Nicotiana benthamiana, achieving a yield of 3.69 mg/g of plant dry weight using the mutant PpUGT91BP1. Taken together, our results deepen the understanding of the PpUGT91 family's role in polyphyllin biosynthesis in P. polyphylla, facilitating rational selection of better P. polyphylla cultivars and guiding future studies in the metabolic engineering of polyphllins.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"249-259"},"PeriodicalIF":3.9,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142838969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Osr40g3 Imparts Salt Tolerance by Regulating GF14e-mediated Gibberellin Metabolism to Activate EG45 in Rice.

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-02-22 DOI: 10.1093/pcp/pcaf023

Chandan Roy, Salman Sahid, Jadav Debgupta, Ananya Roy, Dibyendu Shee, Riddhi Datta, Soumitra Paul

{"title":"Osr40g3 Imparts Salt Tolerance by Regulating GF14e-mediated Gibberellin Metabolism to Activate EG45 in Rice.","authors":"Chandan Roy, Salman Sahid, Jadav Debgupta, Ananya Roy, Dibyendu Shee, Riddhi Datta, Soumitra Paul","doi":"10.1093/pcp/pcaf023","DOIUrl":"https://doi.org/10.1093/pcp/pcaf023","url":null,"abstract":"Under changing environmental conditions, salt stress is a serious threat to agriculture. The R40 family lectins are known to be associated with osmotic stress response, although their mechanism of action remains unclear. Here, we report that Osr40g3, upregulated by salt, improves salt tolerance but causes pollen sterility and poor seed development in rice when constitutively overexpressed. Osr40g3 knockout lines, on the other hand, display salt sensitivity with no other phenotypic aberration. Further studies revealed that the gene exhibits a precise tissue-specific expression pattern essential for seed development. Overexpressing the Osr40g3 with its native promoter alleviates pollen sterility and improves salt tolerance. In-depth molecular studies demonstrated that Osr40g3 positively regulates an expansin protein, OsEG45, while decreasing the stability of a 14-3-3 protein, OsGF14e. Correspondingly, OsEG45 overexpression, OsGF14e silencing lines and the double mutants display salt tolerance, supporting the involvement of the Osr40g3-OsGF14e-OsEG45 module in salt tolerance. Moreover, constitutive overexpression of Osr40g3 or silencing of OsGF14e diminishes gibberellic acid (GA) accumulation that activates the OsEG45 gene. Together, our study highlights that the Osr40g3 confers salt tolerance by negatively regulating OsGF14e while positively regulating OsEG45 via a GA-dependent pathway.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CYP722A1-mediated 16-hydroxylation of carlactonoic acid regulates the floral transition in Arabidopsis.

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-02-19 DOI: 10.1093/pcp/pcaf022

Masaki Kuno, Ayumi Miyamoto, Hinako Takano, Masato Homma, Nanami Shiotani, Kiyono Uchida, Hirosato Takikawa, Masatoshi Nakajima, Masaharu Mizutani, Takatoshi Wakabayashi, Yukihiro Sugimoto

{"title":"CYP722A1-mediated 16-hydroxylation of carlactonoic acid regulates the floral transition in Arabidopsis.","authors":"Masaki Kuno, Ayumi Miyamoto, Hinako Takano, Masato Homma, Nanami Shiotani, Kiyono Uchida, Hirosato Takikawa, Masatoshi Nakajima, Masaharu Mizutani, Takatoshi Wakabayashi, Yukihiro Sugimoto","doi":"10.1093/pcp/pcaf022","DOIUrl":"https://doi.org/10.1093/pcp/pcaf022","url":null,"abstract":"Strigolactones (SLs) are multifunctional plant hormones and rhizosphere signals with diverse structures, roughly classified into two categories: canonical and noncanonical SLs. In Arabidopsis thaliana, SL biosynthesis mutants exhibit increased shoot branching and early flowering, underscoring their roles in developmental regulation. Shoot branching inhibition in Arabidopsis is associated with the methylation of a noncanonical SL, carlactonoic acid (CLA), catalyzed by CLA methyltransferase (CLAMT). Canonical SLs primarily function as rhizosphere signals, with their biosynthesis in dicots mediated by CYP722C enzymes. It is hypothesized that Arabidopsis does not produce canonical SL because of the lack of the CYP722C genes in its genome. Instead, Arabidopsis possesses CYP722A1, a member of the previously uncharacterized CYP722A subfamily, distinct from the CYP722C subfamily. This study demonstrates that Arabidopsis cyp722a1 mutants exhibit an earlier floral transition without excessive shoot branching. Biochemical analysis revealed that CYP722A1 catalyzes the hydroxylation of CLA to produce 16-hydroxy-CLA (16-HO-CLA), which is subsequently methylated by CLAMT to form 16-HO-MeCLA. 16-HO-CLA and 16-HO-MeCLA were detected in the wildtype; however, these compounds were absent in max1-4 mutant, deficient in CLA synthesis, and in cyp722a1 mutant. These findings show CYP722A1-dependent 16-hydroxylation activity of CLA in Arabidopsis. Moreover, they suggest that hydroxylated CLA specifically regulates floral transition, distinct from branching inhibition. Through the identification of CYP722A1 affecting floral transition, which is the distinct role of the CYP722A subfamily, this work provides insights into the structural diversification of SLs for specialized biological functions in plant development.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Involvement of a NIMA-related kinase in cell division in a liverwort Marchantia polymorpha.

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-02-17 DOI: 10.1093/pcp/pcaf021

Hikari Mase, Aoi Sumiura, Yoshihiro Yoshitake, Takayuki Kohchi, Taku Takahashi, Hiroyasu Motose

{"title":"Involvement of a NIMA-related kinase in cell division in a liverwort Marchantia polymorpha.","authors":"Hikari Mase, Aoi Sumiura, Yoshihiro Yoshitake, Takayuki Kohchi, Taku Takahashi, Hiroyasu Motose","doi":"10.1093/pcp/pcaf021","DOIUrl":"https://doi.org/10.1093/pcp/pcaf021","url":null,"abstract":"NIMA-related kinases (NEKs) regulate a series of mitotic events in fungi and animals, whereas plant NEKs have been shown to control the growth direction of cells and organs. Plant NEKs are highly expressed in the meristem, but whether they regulate meristematic activity remains unknown. The liverwort Marchantia polymorpha has a single functional MpNEK1 gene, and its knockout results in twisted rhizoid growth. For a gain-of-function approach, we generated lines for the inducible expression of MpNEK1 using an estrogen receptor mediated system. Estradiol treatment effectively induced the accumulation of MpNEK1 mRNA and MpNEK1-Citrine fusion protein throughout the plant. MpNEK1 overexpression severely suppressed rhizoid and thallus growth, ultimately leading to the lethality of juvenile plants. This severe effect was observed even at the nanomolar level of estradiol. EdU staining and microtubule imaging clearly indicated suppression of cell division by estradiol-induced MpNEK1. MpNEK1 induction also reduced cortical microtubule density and dynamics, but not severely affected cell growth and morphology in thalli. Overexpression of kinase-deficient MpNEK1 also suppressed thallus and rhizoid growth, although to a slightly lesser extent than wild-type MpNEK1, indicating a phosphorylation-independent mechanism of growth suppression. Furthermore, Mpnek1 mutants exhibited growth suppression in their reproductive organs, the gametangiophores. This supports the role of MpNEK1 in cell division, as observed in both fungi and animals.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143441792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Abiotic stress-regulated LEA gene mediates the response to drought, salinity, and cold stress in Medicago sativa L.

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-02-10 DOI: 10.1093/pcp/pcaf020

Xiaoyu Wang, Yulu Hu, Ying Dong, Linsheng Zhang, Bo Wang

{"title":"Abiotic stress-regulated LEA gene mediates the response to drought, salinity, and cold stress in Medicago sativa L.","authors":"Xiaoyu Wang, Yulu Hu, Ying Dong, Linsheng Zhang, Bo Wang","doi":"10.1093/pcp/pcaf020","DOIUrl":"https://doi.org/10.1093/pcp/pcaf020","url":null,"abstract":"Late embryogenesis abundant (LEA) proteins are typical stress-related proteins widely distributed across various organisms. Their anti-stress functions in higher plants have garnered significant attention and have been extensively studied; however, no such studies have been reported on the entire protein family in Medicago sativa. In this study, we identified a total of 83 MsLEA proteins in M. sativa and conducted a comprehensive analysis to elucidate their functions in response to abiotic stresses. The results indicated that these proteins could be classified into seven groups and were distributed across eight chromosomes. Collineation analysis revealed that segmental duplication primarily drove the expansion of MsLEA genes. Furthermore, the promoters of MsLEA genes were found to be enriched with cis-elements associated with various stress responses. Through transcriptome and qRT-PCR analysis, nine MsLEA genes related to drought, salinity, and cold stress were identified, with MsLEA69 selected for further validation. The ectopic expression of MsLEA69 improves osmotic and extreme temperature tolerance by increasing the activity of stress-related enzymes in both prokaryotic and eukaryotic cells. These comprehensive analyses and identifications lay the groundwork for future research into the functional mechanisms of MsLEA proteins and offer potential candidate genes for enhancing resistance breeding in M. sativa.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143383285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An alternative pathway to starch granule initiation unraveled in Chlamydomonas reinhardtii.

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2025-02-07 DOI: 10.1093/pcp/pcaf019

Adeline Courseaux, Philippe Deschamps, David Dauvillée

{"title":"An alternative pathway to starch granule initiation unraveled in Chlamydomonas reinhardtii.","authors":"Adeline Courseaux, Philippe Deschamps, David Dauvillée","doi":"10.1093/pcp/pcaf019","DOIUrl":"https://doi.org/10.1093/pcp/pcaf019","url":null,"abstract":"The initiation of starch granule synthesis remains a relatively poorly understood phenomenon. Recent advances enabled the establishment of a model explaining the synthesis of new starch granules in Arabidopsis thaliana. These characterizations revealed the involvement of both a specific starch synthase isoform (SS4) and of several non-enzymatic proteins in this process. In this work, we investigated whether the initiation of starch synthesis in the green microalgae Chlamydomonas reinhardtii involves the same machinery as those uncovered in the plant model. Our extensive phylogenetic analysis revealed that most of the key players that were identified in higher plants are not found in microalgae suggesting that a different pathway is taking place. We showed that restoration of ADP-glucose synthesis in a mutant strain devoid of any endogenous primers allowed normal starch synthesis, revealing the existence of an initiation mechanism in Chlamydomonas. Our biochemical characterizations revealed that starch synthase isoform 3 possesses the intrinsic capacity to initiate polysaccharide synthesis in vitro and could be one of the functions involved in starch initiation. Our work suggests that the initiation of starch synthesis in Chlamydomonas involves a different pathway to that described in Arabidopsis and that further efforts will be required to identify the proteins involved in this process.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143365465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0