Plant and Cell Physiology最新文献

{"title":"ABCG transporters are involved in the accumulation of specialized metabolites in the oil bodies of Marchantia polymorpha.","authors":"Takehiko Kanazawa, Tomoko Kawaguchi, Yuta Moriwaki, Kan-Ichi Iwasaki, Takashi Ueda, Kenji Matsui","doi":"10.1093/pcp/pcag069","DOIUrl":"10.1093/pcp/pcag069","url":null,"abstract":"<p><p>Bioactive specialized metabolites (SMs) are synthesized and sequestered in specific cellular compartments or organelles as a self-defense strategy against their intrinsic toxicity. Liverwort-specific oil bodies accumulate large amounts of SMs and contribute to chemical defense; however, the molecular mechanisms underlying SM sequestration in oil bodies remain largely unknown. Therefore, in this study, we focused on MpABCG1 and MpABCG36, which are ATP-binding cassette (ABC) protein family members localized to the oil bodies of liverwort Marchantia polymorpha. Sesquiterpene (thujopsene, chamigrene, and himachalene) accumulation was reduced in the Mpabcg1 and Mpabcg36 loss-of-function mutants. Notably, levels of the bisbibenzyls, marchantins C and A, were predominantly reduced in Mpabcg1, but not in Mpabcg36. Although the Mpabcg1 mutant formed a number of oil bodies labeled with mCitrine-MpSYP12B (an oil body membrane marker) comparable to that of the wild type, the number of oil bodies stained with BODIPY 493/503, which has an affinity for lipophilic SMs, was reduced. This finding suggests that MpABCG1 and MpABCG36 mutations affect SM accumulation in the oil body but have little impact on oil body formation. Overall, our results highlight the involvement of MpABCG1 and MpABCG36 in the accumulation of SMs and/or their precursors in liverwort oil bodies.</p>","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"1442-1454"},"PeriodicalIF":4.7,"publicationDate":"2026-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13545645/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148006483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arabidopsis TITAN-LIKE is required for U12-type intron splicing, especially of AT-AC subtypes.","authors":"Tomoko Niwa, Junshin Miyamoto, Nao Iwase, Haruka Iwai, Takaaki Kojima, Takamasa Suzuki","doi":"10.1093/pcp/pcag057","DOIUrl":"10.1093/pcp/pcag057","url":null,"abstract":"<p><p>Many eukaryotes possess two types of spliceosomes: the U2-dependent and U12-dependent spliceosomes. The U2-dependent spliceosome processes >99% of all introns, whereas the U12-dependent spliceosome acts on only ~0.3% of introns, one-third of which start with AT and end with AC, with the remainder having GT-AG termini. How the U12-dependent spliceosome splices two types of introns with different terminal sequences remains poorly understood. Human centrosomal AT-AC splicing factor (CENATAC) is a subunit of the U12-dependent spliceosome that is particularly required for the splicing of the AT-AC subtype. The Arabidopsis genome contains a single homolog, TITAN-LIKE (TTL), but its function in splicing remains unknown. Here, we generated ttl mutants and isolated two viable alleles, of which we analyzed one, designated ttl-142, to investigate TTL's function in splicing. ttl-142 carries a 42-nucleotide deletion that removes 14 amino acid residues from the predicted protein, and homozygous mutants exhibit morphological abnormalities. Most U12-dependent introns were less efficiently spliced in ttl-142 than in the wild type, with the splicing of AT-AC introns particularly suppressed. Splicing suppression in ttl-142 was more extensive than in a drol1 (defective repression of the OLE3:LUC1) mutant, which carries a mutation in a gene specifically required for AT-AC intron splicing. Conversely, fewer genes showed altered expression levels in ttl-142 than in drol1, and most differentially expressed genes differed between the two mutants. These results suggest that the phenotypes of ttl-142 and drol1 mutants may reflect the impairment of distinct spliceosomal functions.</p>","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"1356-1367"},"PeriodicalIF":4.7,"publicationDate":"2026-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147779367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptional control of gemma dormancy via dual pathways in Marchantia polymorpha.","authors":"Nami Yoshimura, Hirotaka Kato, Mikako Yoshikawa, Yuuki Sakai, Hideyuki Matsuura, Kosaku Takahashi, Daisuke Takezawa, Takehiko Kanazawa, Tomoyuki Furuya, Takashi Ueda, Yuki Kondo, Hidehiro Fukaki, Kimitsune Ishizaki","doi":"10.1093/pcp/pcag071","DOIUrl":"10.1093/pcp/pcag071","url":null,"abstract":"<p><p>Dormancy is a crucial trait that allows land plants to withstand harsh terrestrial environments by pausing growth until favorable conditions return. The liverwort Marchantia polymorpha produces dormant propagules, gemmae, for asexual reproduction. The phytohormone ABA is known to regulate dormancy in the seeds of flowering plants and the gemmae of M. polymorpha. Here, we identify a basic helix-loop-helix protein, MpHYPNOS (MpHYP), as a central regulator of gemma dormancy. MpHYP expression induces germination arrest in gemmae. Transcriptome analysis revealed that MpHYP represses a D-type cyclin gene MpCYCD;1 and induces genes involved in ABA biosynthesis. Repression of MpCYCD;1 expression and germination arrest are both maintained even in an ABA-receptor mutant. By contrast, Mphyp and ABA-related mutants lack the desiccation tolerance observed in wild-type gemmae. We propose that MpHYP regulates gemma dormancy through two parallel pathways: an ABA-independent pathway that suppresses germination through cell cycle repression, and an ABA-dependent pathway that enhances desiccation tolerance via ABA biosynthesis. These findings highlight an integrated regulatory mechanism of dormancy via phytohormonal and cell cycle control in bryophytes.</p>","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"1287-1298"},"PeriodicalIF":4.7,"publicationDate":"2026-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148034197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic profiling of the response to high night-time temperature stress in tomato leaves.","authors":"Huihui Fang, Kaixin Zheng, Yijie Zang, Yunfei Xu, Xiaofang Zhang, Weiling Zhao, Wenjia Chen, Yi Wang, Minglu Lu, Yunxiang Zang","doi":"10.1093/pcp/pcag058","DOIUrl":"10.1093/pcp/pcag058","url":null,"abstract":"<p><p>High night-time temperature (HNT) poses a major challenge to tomato (Solanum lycopersicum L.) growth and productivity. To elucidate the molecular basis of HNT responses, this study systematically examined the morphological and transcriptomic changes in tomato seedlings under prolonged HNT stress. We observed that HNT suppressed plant growth and chlorophyll content while triggering H2O2 accumulation in new leaves; concurrently, it promoted thermomorphogenesis-related adaptations, such as reduced leaf angles and lower leaf trichome density; traits potentially facilitating heat dissipation. Transcriptome profiling identified 4551 differentially expressed genes (DEGs), comprising 2104 up-regulated and 2447 down-regulated genes. Functional enrichment analysis revealed that up-regulated DEGs were primarily involved in glycosyl transfer, flavonoid biosynthesis, mismatch repair, and protein processing, whereas down-regulated DEGs were enriched in photosynthesis, metabolic, and immune signaling. These changes suggest a strategic trade-off, with down-regulated photosynthetic and metabolic activities potentially enabling the reallocation of resources toward stress resilience mechanisms. As a central heat shock response mechanism, the SlHSPs-SlHSFs system responded to HNT, with 10-day stress inducing distinct expression patterns of SlHSP70/90 genes alongside concurrent suppression of SlHSFs. Reverse transcription quantitative PCR (RT-qPCR) analysis unveiled a transcriptional shift in SlHSFs from an initial shock phase, marked by pronounced expression changes at 1-day HNT, to a sustained acclimation phase. Prolonged HNT also triggered gene-specific expression changes in the unfolded protein response pathway, as well as in genes involved in reactive oxygen species homeostasis and hormone signaling. In addition, it increased alternative splicing in genes associated with antioxidant defense, DNA repair, and protein processing. Collectively, these transcriptomic alterations reflect a systemic reprogramming that prioritizes energy conservation, redox homeostasis, and macromolecular stability to support nocturnal heat acclimation. Our findings provide novel insights into tomato adaptation to HNT and offer valuable genetic resources and a theoretical foundation for breeding HNT-resilient tomato varieties.</p>","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"1368-1384"},"PeriodicalIF":4.7,"publicationDate":"2026-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147842135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Untangling the evolutionary history of SABATH enzymes in non-seed plants.","authors":"Tetsuya Hisanaga","doi":"10.1093/pcp/pcag106","DOIUrl":"10.1093/pcp/pcag106","url":null,"abstract":"","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"1256-1258"},"PeriodicalIF":4.7,"publicationDate":"2026-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148631249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving perennial ryegrass transformation protocols with developmental regulators.","authors":"Jonathan P Cors, Gabriel W Olson-Jensen, Michael J Smanski, Eric Watkins","doi":"10.1093/pcp/pcag064","DOIUrl":"10.1093/pcp/pcag064","url":null,"abstract":"","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"1259-1262"},"PeriodicalIF":4.7,"publicationDate":"2026-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147988619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Starch phosphorylase acts as a pivotal scaffold connecting starch metabolism enzymes with photosystem I subunits.","authors":"Seon-Kap Hwang, Li Tian, Chun-Yeung Ng, Thomas W Okita","doi":"10.1093/pcp/pcag063","DOIUrl":"10.1093/pcp/pcag063","url":null,"abstract":"<p><p>In a previous study, we proposed that starch phosphorylase (Pho1) interacts with PsaC, a core subunit of photosystem I (PSI), although it was not clear whether the interaction was a direct 1:1 relationship or by indirect association with another factor. Here, we validated the Pho1-PsaC interaction in vivo using bimolecular fluorescence complementation analysis. Pulldown assays with wild-type, BMF136, and the Pho1-null plants revealed that glutathione S-transferase-Pho1 consistently associates with PsaC and the starch disproportionating enzyme (Dpe1) across all genotypes. These results indicate that Pho1 is essential for forming a multi-protein complex containing PsaC and Dpe1. Yeast 2-hybrid assays further validated the direct Pho1-PsaC interaction and also demonstrated that Pho1 interacts with PsaD, another subunit of PSI, but not with PsaE, indicating that PsaD functions as an additional component of the Pho1-associated heterocomplex. PsaC bound poorly to the Pho1 catalytically null variants, although PsaC fused to a HaloTag restored interaction with the catalytic null mutants, implying that the HaloTag facilitated or stabilized complex formation. Our results indicate that Pho1 is an essential factor linking starch biosynthesis to photosynthesis through its ability to form multi-protein complexes with starch metabolism enzymes and PSI subunits.</p>","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"1430-1441"},"PeriodicalIF":4.7,"publicationDate":"2026-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13545646/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147941955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional insights into a putative 2-OG oxygenase involved in jasmonic acid metabolism and xylem development in Populus trichocarpa.","authors":"Natalia Wojciechowska, Maciej Janicki, Katarzyna Marzec-Schmidt, Thierry Heitz, Magdalena Ślachetka, Agnieszka Ludwików, Agnieszka Bagniewska-Zadworna","doi":"10.1093/pcp/pcag035","DOIUrl":"10.1093/pcp/pcag035","url":null,"abstract":"<p><p>The xylem vascular tissue constitutes an efficient system of long-distance transport of water and nutrients, as well as structural support to the plant body. Despite its importance, the molecular mechanisms regulating xylogenesis remain only partially understood. In this study, we investigated two genes from Populus trichocarpa encoding 2-oxoglutarate (2-OG) and Fe(II)-dependent oxygenases, whose expression is significantly upregulated during secondary growth. Proteins encoded by those genes share similarities with Arabidopsis enzymes known as jasmonic acid oxygenases (JAOs) or jasmonate-induced oxygenases, which are involved in the hydroxylation of jasmonic acid (JA)-a hormone precursor critical for plant development and stress responses. We hypothesized that the Populus 2-OG oxygenases participate in JA metabolism and thereby influence wood formation. Their in silico structural analysis indicated that one of these proteins likely binds JA with similar efficiency to known JAO proteins. A link was supported by in vitro and in vivo experiments. Studies in an Arabidopsis JAO-depleted mutant revealed altered stem anatomy, characterized by a reduced stem diameter and an increased proportion of xylem area relative to the total stem cross-section, suggesting that disrupted JA metabolism can interfere with normal vascular development. These findings provide new insights into the hormonal regulation of vascular development and may have practical implications for improving wood quality or stress resilience in trees.</p>","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"1299-1315"},"PeriodicalIF":4.7,"publicationDate":"2026-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13545655/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147463776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PaMYB55 regulates cellulose and lignin deposition to control floral organ development, cell wall integrity, and pathogen resistance in Phalaenopsis orchids.","authors":"Wan-Ting Mao, Wei-Han Hsu, Pei-Tzu Lin, Chang-Hsien Yang","doi":"10.1093/pcp/pcag062","DOIUrl":"10.1093/pcp/pcag062","url":null,"abstract":"<p><p>R2R3-MYB transcription factors are key regulators of plant development and secondary cell wall biosynthesis, yet their roles in orchids remain poorly understood. Here, we identify and functionally characterize PaMYB55 from Phalaenopsis Sogo Yukidian V3, an ortholog of Arabidopsis MYB55, predominantly expressed during late flower development. Virus-induced gene silencing (VIGS) of PaMYB55 resulted in smaller sepals and petals due to reduced cell expansion with decreased expression of expansion-related genes (PaEXPA10-like and PaXTH6), accompanied by altered epidermal integrity, sunken striations, and premature floral senescence. Histochemical staining and quantitative assays revealed decreased cellulose and lignin deposition in both floral organs and peduncles, correlating with downregulation of cellulose synthase (PaCESA4, PaCESA7, PaCESA9) and lignin biosynthetic genes (PaPAL1, PaC4H, PaCCR1, PaCCoAMT, PaCOMT1-3, PaF5H, PaCAD2). Furthermore, PaMYB55 silencing compromised pathogen restriction, as shown by enhanced spread of green fluorescent protein-tagged Cymbidium mosaic virus beyond vascular bundles into mesophyll tissues, and upregulated immune-related genes. Our findings demonstrate that PaMYB55 positively regulates cell expansion and cell wall integrity by promoting lignin and cellulose biosynthesis, thereby contributing to floral morphology, tissue structural stability, and defense against pathogen spread. This work advances our understanding of MYB functions in orchids and highlights PaMYB55 as a potential target for improving both ornamental traits and disease resistance in breeding programs.</p>","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"1414-1429"},"PeriodicalIF":4.7,"publicationDate":"2026-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147942695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Grass Expression Atlas: an RNA-seq-based expression resource for grass species.","authors":"Kota Kambara, Qi Chen, Daisuke Tsugama","doi":"10.1093/pcp/pcag122","DOIUrl":"https://doi.org/10.1093/pcp/pcag122","url":null,"abstract":"<p><p>Grass Expression Atlas (GExA) is an interactive web-based resource for rapid exploration of gene expression across diverse tissues, developmental stages, and conditions in grass species. GExA integrates publicly available RNA sequencing (RNA-seq) datasets for four millets: pearl millet (Cenchrus americanus), foxtail millet (Setaria italica), proso millet (Panicum miliaceum), and finger millet (Eleusine coracana), and includes barley (Hordeum vulgare), sorghum (Sorghum bicolor) and rice (Oryza sativa) as reference species. Public RNA-seq datasets were reanalyzed using a unified workflow within each species to obtain read counts for each gene, which were then converted into consistently formatted expression matrices in transcripts per million (TPM). The current release comprises 4,753 samples from 457 BioProjects, including 987 pearl millet samples and 2,216 foxtail millet samples, and is provided through a user-friendly web interface. GExA is designed for scalable expansion to additional species via the pipeline used in this study. GExA is freely available athttps://www.gexa.anesc.u-tokyo.ac.jp/.</p>","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148881403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}