Plant and Cell Physiology最新文献_第5页

Extracellular vesicle-mediated secretion of chlorophyll biosynthetic intermediates in the cyanobacterium Leptolyngbya boryana. 蓝藻 Leptolyngbya boryana 细胞外囊泡介导的叶绿素生物合成中间体分泌。

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2024-08-22 DOI: 10.1093/pcp/pcae095

Kentaro Usui, Haruki Yamamoto, Hitoshi Mori, Yuichi Fujita

{"title":"Extracellular vesicle-mediated secretion of chlorophyll biosynthetic intermediates in the cyanobacterium Leptolyngbya boryana.","authors":"Kentaro Usui, Haruki Yamamoto, Hitoshi Mori, Yuichi Fujita","doi":"10.1093/pcp/pcae095","DOIUrl":"https://doi.org/10.1093/pcp/pcae095","url":null,"abstract":"Extracellular vesicles (EVs) are derived from the outer membrane (OM) in Gram-negative bacteria and have diverse physiological functions. EV-mediated secretion of monovinyl protochlorophyllide (MV-Pchlide), the chlorophyll a (Chl) biosynthetic intermediate, was previously reported in a mutant lacking dark-operative Pchlide reductase in the cyanobacterium Leptolyngbya boryana. This study showed a detailed characterization of EVs from the wild-type (WT) of L. boryana grown under photoautotrophic and dark heterotrophic conditions, focusing on the accumulation of Chl intermediates. WT L. boryana cells produce two types of EVs, low-density EVs (L-EVs) and high-density EVs (H-EVs), both under light and dark conditions. L-EVs and H-EVs showed distinct morphological features and protein compositions. L-EVs from cells grown under both light and dark conditions commonly contained carotenoids, ketomyxol glycoside, and zeaxanthin, as major pigments. Based on the protein compositions of EVs and other cellular membrane fractions, L-EVs and H-EVs are probably derived from low-density OM and high-density OM interacting with cell walls, respectively. Fluorescence detection of pigments was applied to EVs, and the two Chl intermediates, protoporphyrin IX and protoporphyrin IX monomethyl ester, were commonly detected in both L-EVs from light- and dark-grown cells, whereas L-EVs from dark-grown cells contained additional MV-Pchlide, MV-protopheophorbide, and pheophorbide. The pigment ratios of L-EVs to the total culture medium of the Chl intermediates were much higher than those of carotenoids, suggesting an active transport of the Chl intermediates from the thylakoid membrane to L-EVs. Cyanobacterial EVs may play a novel role in alleviating the accumulation of Chl intermediates in cells. (248 words).","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142036758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Natural variation in sexual traits and gene expression between selfing and outcrossing Arabidopsis lyrata suggests sexual selection at work. 自交和外交拟南芥在性状和基因表达方面的自然差异表明，性选择在起作用。

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2024-08-10 DOI: 10.1093/pcp/pcae090

Ömer İltaş, Martin Čertner, Clément Lafon Placette

{"title":"Natural variation in sexual traits and gene expression between selfing and outcrossing Arabidopsis lyrata suggests sexual selection at work.","authors":"Ömer İltaş, Martin Čertner, Clément Lafon Placette","doi":"10.1093/pcp/pcae090","DOIUrl":"https://doi.org/10.1093/pcp/pcae090","url":null,"abstract":"Flowering plants show significant diversity in sexual strategies, profoundly impacting the evolution of sexual traits and associated genes. Sexual selection is one of the primary evolutionary forces driving sexual trait variation, particularly evident during pollen-pistil interactions, where pollen grains compete for fertilization and females select mating partners. Multiple mating may intensify competition among pollen donors for siring, while in contrast, self-fertilization reduces sire-sire competition, relaxing the sexual selection pressure. Traits involved in male-male competition and female choice are well described, and molecular mechanisms underlying pollen development and pollen-pistil interactions have been extensively studied in the model species Arabidopsis thaliana. However, whether these molecular mechanisms are involved in sexual selection in nature remains unclear. To address this gap, we measured intrinsic pollen performance and its interaction with female choice, and investigated the associated gene expression patterns in a selfing and an outcrossing population of Arabidopsis lyrata. We found that pollen germination and pollen tube growth were significantly higher in outcrossers than selfers, and this difference was accompanied by changes in expression of genes involved in vesicle transport and cytoskeleton. Outcrosser mother plants showed a negative impact on pollen tube growth compared to selfer mother plants, together with a difference of expression for genes involved in auxin and stress response, suggesting a potential mechanism for female choice through molecular crosstalk at the post-pollination stage. Our study provides insight on the impact of sexual selection on the evolution of sexual gene expression in plants.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141913747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytochrome C and Low Temperature Promote the Protein Accumulation and Red Light Signaling of Phytochrome D. 植物色素 C 和低温促进植物色素 D 的蛋白质积累和红光信号传递

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2024-08-09 DOI: 10.1093/pcp/pcae089

Csaba Péter, Éva Ádám, Cornelia Klose, Gábor Grézal, Anita Hajdu, Gábor Steinbach, László Kozma-Bognár, Dániel Silhavy, Ferenc Nagy, András Viczián

{"title":"Phytochrome C and Low Temperature Promote the Protein Accumulation and Red Light Signaling of Phytochrome D.","authors":"Csaba Péter, Éva Ádám, Cornelia Klose, Gábor Grézal, Anita Hajdu, Gábor Steinbach, László Kozma-Bognár, Dániel Silhavy, Ferenc Nagy, András Viczián","doi":"10.1093/pcp/pcae089","DOIUrl":"https://doi.org/10.1093/pcp/pcae089","url":null,"abstract":"Light affects almost every aspect of plant development. It is perceived by photoreceptors, among which phytochromes (PHY) are responsible for monitoring the red and far-red spectrum. Arabidopsis thaliana possesses five phytochrome genes (phyA-E). Whereas functions of phyA and phyB are extensively studied, our knowledge on other phytochromes is still rudimentary. To analyze phyD function we expressed it at high levels in different phytochrome-deficient genetic backgrounds. Overexpressed phyD-YFP can govern effective light signaling but only at low temperature and in cooperation with functional phyC. Under these conditions, phyD-YFP accumulates to high levels and opposite to phyB, this pool is stable in light. By comparing the photoconvertible phyD-YFP and phyB levels and their signaling in continuous and pulsed irradiation, we showed that phyD-YFP is a less efficient photoreceptor than phyB. This conclusion is supported by the facts that only a part of the phyD-YFP pool is photoconvertible and thermal reversion of phyD-YFP is faster than that of phyB. Our data suggest that the temperature-dependent function of phyD is based on the amount of phyD protein and not on its Pfr stability, as described for phyB. We also found that phyD-YFP and phyB-GFP associate with strongly overlapping genomic locations and mediate similar changes in gene expression, however the efficiency of phyD-YFP is lower. Based on these data we propose that under certain conditions, synergistic interaction of phyD and phyC can substitute phyB function in seedlings and in adult plants, thus increases the ability of plants to respond more flexibly to environmental changes.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141907573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Jasmonic Acid Biosynthetic Genes SmLOX4 and SmLOX5 are Involved in Heat Tolerance in Eggplant. 茉莉酸合成基因 SmLOX4 和 SmLOX5 参与了茄子的耐热性。

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2024-08-09 DOI: 10.1093/pcp/pcae088

Renjian Liu, Bingbing Shu, Yuyuan Wang, Jiazhu Feng, Bingwei Yu, Yuwei Gan, Yonggui Liang, Zhengkun Qiu, Shuangshuang Yan, Bihao Cao

{"title":"The Jasmonic Acid Biosynthetic Genes SmLOX4 and SmLOX5 are Involved in Heat Tolerance in Eggplant.","authors":"Renjian Liu, Bingbing Shu, Yuyuan Wang, Jiazhu Feng, Bingwei Yu, Yuwei Gan, Yonggui Liang, Zhengkun Qiu, Shuangshuang Yan, Bihao Cao","doi":"10.1093/pcp/pcae088","DOIUrl":"https://doi.org/10.1093/pcp/pcae088","url":null,"abstract":"High temperature stress (HTS) affects the growth and production of vegetable crops, including eggplant (Solanum melongena L.). Jasmonic acid (JA) plays key roles in regulating resistance to biotic and abiotic stresses in plants. Nonetheless, reports on the role of JA in heat tolerance in eggplant are rare. Herein, the effects of JA on heat tolerance in eggplant and the functions of the JA biosynthetic genes SmLOX4 and SmLOX5 were analysed. The results showed that the JA content increased under high temperature treatment (HTT) and that exogenous methyl jasmonate (MeJA) treatment reduced the damage caused by HTT to eggplant. The expression of SmLOX4 and SmLOX5 was induced by HTT and was significantly positively correlated with JA biosynthesis. SmLOX4 and SmLOX5 were localized in chloroplasts. The silencing of SmLOX4 and SmLOX5 by virus-induced gene silencing (VIGS) suppressed the heat tolerance of eggplant plants, whereas the overexpression of SmLOX4 and SmLOX5 enhanced the heat tolerance of Arabidopsis thaliana plants. JA content and the expression of JA signalling-related genes decreased in the SmLOX4- and SmLOX5-silenced plants but increased in the OE-SmLOX4 and OE-SmLOX5 transgenic plants. These results revealed that SmLOX4 and SmLOX5 improved eggplant heat tolerance by mediating JA biosynthesis and JA signalling pathways.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141907574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ozone priming enhanced low temperature tolerance of wheat (Triticum aestivum L.) based on physiological, biochemical and transcriptional analyses. 基于生理、生化和转录分析的臭氧引物增强了小麦（Triticum aestivum L.）的低温耐受性。

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2024-08-03 DOI: 10.1093/pcp/pcae087

Bing Dai, Hongyan Wang, Weiqiang Li, Peng Zhang, Tianhao Liu, Xiangnan Li

{"title":"Ozone priming enhanced low temperature tolerance of wheat (Triticum aestivum L.) based on physiological, biochemical and transcriptional analyses.","authors":"Bing Dai, Hongyan Wang, Weiqiang Li, Peng Zhang, Tianhao Liu, Xiangnan Li","doi":"10.1093/pcp/pcae087","DOIUrl":"https://doi.org/10.1093/pcp/pcae087","url":null,"abstract":"Low temperature significantly inhibits the plant growth in wheat (Triticum aestivum L.), prompting the exploration of effective strategies to mitigate low temperature stress. Several priming methods enhance low temperature stress tolerant, however, the role of ozone priming remains unclear in wheat. Here we found ozone priming alleviated low temperature stress in wheat. Transcriptome analysis showed that ozone priming positively modulated 'photosynthesis-antenna proteins' pathway in wheat under low temperature. Which was confirmed by the results of the ozone-primed plants had higher trapped energy flux and electron transport flux per reaction, and less damage to chloroplasts than non-primed plants under low temperature. Ozone priming also mitigated the overstimulation of glutathione metabolism and induced the accumulation of total ascorbic acid and glutathione, maintained redox homeostasis in wheat under low temperature. Moreover, gene expressions and enzyme activities in glycolysis pathways were upregulated in ozone priming comparing with non-priming after the low temperature stress. Furthermore, exogenous antibiotics significantly increased low temperature tolerance, which further proved that the inhibition of ribosome biogenesis by ozone priming was involved in low temperature tolerance in wheat. In conclusion, ozone priming enhanced wheat low temperature tolerance through promoting light-harvesting capacity, redox homeostasis, and carbohydrate metabolism, as well as inhibiting ribosome biogenesis.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Probing bilin-protein interaction in the protochromic photocycle of cyanobacteriochrome RcaE by site-directed mutagenesis. 通过定点突变探究蓝藻色素 RcaE 的原色光周期中的双蛋白-蛋白相互作用。

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2024-08-02 DOI: 10.1093/pcp/pcae085

Takanari Kamo, Takaaki Matsushita, Masako Hamada, Tomotsumi Fujisawa, Toshihiko Eki, Masashi Unno, Yuu Hirose

{"title":"Probing bilin-protein interaction in the protochromic photocycle of cyanobacteriochrome RcaE by site-directed mutagenesis.","authors":"Takanari Kamo, Takaaki Matsushita, Masako Hamada, Tomotsumi Fujisawa, Toshihiko Eki, Masashi Unno, Yuu Hirose","doi":"10.1093/pcp/pcae085","DOIUrl":"https://doi.org/10.1093/pcp/pcae085","url":null,"abstract":"Cyanobacteriochromes (CBCRs) are members of the phytochrome superfamily of photosensor proteins that bind a bilin chromophore. CBCRs exhibit substantial diversity in their absorption wavelengths through a variety of bilin-protein interactions. RcaE is the first discovered cyanobacteriochrome as a regulator of chromatic acclimation, where cyanobacteria optimize the absorption wavelength of their photosynthetic antenna. RcaE undergoes a reversible photoconversion between a green-absorbing (Pg) and a red-absorbing (Pr) states, where the bilin chromophore adopts a deprotonated C15-Z,anti and a protonated C15-E,syn structures, respectively. This photocycle is designated as \"protochromic photocycle\" as the change of the bilin protonation state is responsible for the large absorption shift. With the guidance of recently determined Pg and Pr structures of RcaE, in this study, we investigated bilin-chromophore interaction by site-directed mutagenesis on three key residues referred to as a protochromic triad and also other conserved residues interacting with the bilin. Among the protochromic triad residues, Glu217 and Lys261 are critical for the formation of the Pr state, while Leu249 is critical for the formation of both Pg and Pr states. Substitution in other conserved residues, including Val218, Phe219, and Pro220 in the wind-up helix and Phe252, Phe214, and Leu209 in a part of the bilin-binding pocket, had less substantial effects on the spectral sensitivity in RcaE. These data provide insights into our understanding of the bilin-chromophore interaction in the protochromic photocycle and also its evolution in the CBCRs.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141875771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Chaperone NASP Contributes to de Novo Deposition of the Centromeric Histone Variant CENH3 in Arabidopsis Early Embryogenesis. 伴侣蛋白 NASP 有助于拟南芥早期胚胎发生过程中中心粒组蛋白变体 CENH3 的新沉积。

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2024-07-30 DOI: 10.1093/pcp/pcae030

Hidenori Takeuchi, Shiori Nagahara, Tetsuya Higashiyama, Frédéric Berger

{"title":"The Chaperone NASP Contributes to de Novo Deposition of the Centromeric Histone Variant CENH3 in Arabidopsis Early Embryogenesis.","authors":"Hidenori Takeuchi, Shiori Nagahara, Tetsuya Higashiyama, Frédéric Berger","doi":"10.1093/pcp/pcae030","DOIUrl":"10.1093/pcp/pcae030","url":null,"abstract":"The centromere is an essential chromosome region where the kinetochore is formed to control equal chromosome distribution during cell division. The centromere-specific histone H3 variant CENH3 (also called CENP-A) is a prerequisite for the kinetochore formation. Since CENH3 evolves rapidly, associated factors, including histone chaperones mediating the deposition of CENH3 on the centromere, are thought to act through species-specific amino acid sequences. The functions and interaction networks of CENH3 and histone chaperons have been well-characterized in animals and yeasts. However, molecular mechanisms involved in recognition and deposition of CENH3 are still unclear in plants. Here, we used a swapping strategy between domains of CENH3 of Arabidopsis thaliana and the liverwort Marchantia polymorpha to identify specific regions of CENH3 involved in targeting the centromeres and interacting with the general histone H3 chaperone, nuclear autoantigenic sperm protein (NASP). CENH3's LoopN-α1 region was necessary and sufficient for the centromere targeting in cooperation with the α2 region and was involved in interaction with NASP in cooperation with αN, suggesting a species-specific CENH3 recognition. In addition, by generating an Arabidopsis nasp knock-out mutant in the background of a fully fertile GFP-CENH3/cenh3-1 line, we found that NASP was implicated for de novo CENH3 deposition after fertilization and thus for early embryo development. Our results imply that the NASP mediates the supply of CENH3 in the context of the rapidly evolving centromere identity in land plants.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11287212/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140867612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual Regulation of Cytochrome P450 Gene Expression by Two Distinct Small RNAs, a Novel tasiRNA and miRNA, in Marchantia polymorpha. Marchantia polymorpha 中两种不同的小 RNA（一种新型 tasiRNA 和 miRNA）对细胞色素 P450 基因表达的双重调控。

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2024-07-30 DOI: 10.1093/pcp/pcae029

Yu-Ling Hung, Syuan-Fei Hong, Wei-Lun Wei, Shiuan Cheng, Jia-Zhen Yu, Veny Tjita, Qian-Yuan Yong, Ryuichi Nishihama, Takayuki Kohchi, John L Bowman, Yuan-Chi Chien, Yen-Hsin Chiu, Ho-Chun Yang, Mei-Yeh Jade Lu, Zhao-Jun Pan, Chun-Neng Wang, Shih-Shun Lin

{"title":"Dual Regulation of Cytochrome P450 Gene Expression by Two Distinct Small RNAs, a Novel tasiRNA and miRNA, in Marchantia polymorpha.","authors":"Yu-Ling Hung, Syuan-Fei Hong, Wei-Lun Wei, Shiuan Cheng, Jia-Zhen Yu, Veny Tjita, Qian-Yuan Yong, Ryuichi Nishihama, Takayuki Kohchi, John L Bowman, Yuan-Chi Chien, Yen-Hsin Chiu, Ho-Chun Yang, Mei-Yeh Jade Lu, Zhao-Jun Pan, Chun-Neng Wang, Shih-Shun Lin","doi":"10.1093/pcp/pcae029","DOIUrl":"10.1093/pcp/pcae029","url":null,"abstract":"The miR390-derived TAS3 trans-acting short-interfering RNAs (tasiRNAs) module represents a conserved RNA silencing pathway in the plant kingdom; however, its characterization in the bryophyte Marchantia polymorpha is limited. This study elucidated that MpDCL4 processes MpTAS3 double-stranded RNA (dsRNA) to generate tasiRNAs, primarily from the 5'- and 3'-ends of dsRNA. Notably, we discovered a novel tasiRNA, tasi78A, which can negatively regulate a cytochrome P450 gene, MpCYP78A101. Additionally, tasi78A was abundant in MpAGO1, and transient expression assays underscored the role of tasi78A in repressing MpCYP78A101. A microRNA, miR11700, also regulates MpCYP78A101 expression. This coordinate regulation suggests a role in modulating auxin signaling at apical notches of gemma, influencing the growth and sexual organ development of M. polymorpha and emphasizing the significance of RNA silencing in MpCYP78A101 regulation. However, phylogenetic analysis identified another paralog of the CYP78 family, Mp1g14150, which may have a redundant role with MpCYP78A101, explaining the absence of noticeable morphological changes in loss-of-function plants. Taken together, our findings provide new insights into the combined regulatory roles of miR390/MpTAS3/miR11700 in controlling MpCYP78A101 and expand our knowledge about the biogenesis and regulation of tasiRNAs in M. polymorpha.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140306658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proposal of Reference Genes for Tomato Fruit Ripening qRT-PCR Data Normalization. 番茄果实成熟 qRT-PCR 数据归一化参考基因的建议。

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2024-07-30 DOI: 10.1093/pcp/pcae051

Ricardo Rivera-Silva, Ricardo A Chávez Montes, Alejandra Cervera, Fabiola Jaimes-Miranda

引用次数: 0

Correction to: Spatio-Temporal Study of Galactolipid Biosynthesis in Duckweed Using Mass Spectrometry Imaging and in vivo Isotope Labeling. 更正：利用质谱成像和体内同位素标记对鸭茅中半乳糖脂生物合成的时空研究

IF 3.9 2区生物学

Plant and Cell Physiology Pub Date : 2024-07-30 DOI: 10.1093/pcp/pcae064

引用次数: 0