Plant and Cell Physiology最新文献_第5页

Molecular architecture for state transition: insights from structural biology and evolutionary trajectories. 状态转变的分子结构：来自结构生物学和进化轨迹的见解。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-09-11 DOI: 10.1093/pcp/pcaf114

Jun Minagawa

{"title":"Molecular architecture for state transition: insights from structural biology and evolutionary trajectories.","authors":"Jun Minagawa","doi":"10.1093/pcp/pcaf114","DOIUrl":"https://doi.org/10.1093/pcp/pcaf114","url":null,"abstract":"Photosynthetic state transitions rapidly reallocate excitation energy between PSI and PSII to maintain redox poise in the thylakoid electron transport chain. This process relies on reversible phosphorylation of LHCII, allowing its transient association with PSI. Cryo-electron microscopy has resolved the structural interface between phosphorylated LHCII and PSI, revealing a conserved RRpT motif that docks to a site formed by PsaH and PsaL proteins. Strikingly, analogous PSI supercomplexes have now been identified in early diverging green lineages, including the bryophyte Physcomitrium patens and the marine prasinophyte Ostreococcus tauri, each displaying lineage-specific adaptations involving the moss-specific antenna protein Lhcb9 and the prasinophyte-specific antenna protein Lhcp, respectively. These findings suggest that the core molecular architecture for state transitions originated early in green plant evolution and was subsequently remodeled in distinct lineages to support adaptation to freshwater and terrestrial habitats. LHCII phosphorylation is primarily regulated by the redox state of the plastoquinone pool and its interaction with the cytochrome b6f complex. Conserved Ser/Thr kinases (Stt7/STN7) and PP2C-type phosphatases (TAP38/PPH1) mediate this process, integrating redox signaling into photosynthetic regulation. The kinase is further modulated by thioredoxin reduced downstream of PSI, adding an additional layer of redox-dependent control. This review synthesizes recent structural, biochemical, and phylogenetic insights, reframing state transition as a photoregulatory strategy that coordinates environmental light sensing with the optimization of energy capture, photoprotection, and adaptive plasticity.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145200771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of regulatory promoter sequences directing MtCP6 transcription at the onset of nodule senescence in Medicago truncatula. 短叶紫花苜蓿结核衰老开始时MtCP6转录调控启动子序列的鉴定。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-09-11 DOI: 10.1093/pcp/pcaf110

Li Yang, Lisa Frances, Fernanda de Carvalho-Niebel, Pierre Frendo, Eric Boncompagni

{"title":"Identification of regulatory promoter sequences directing MtCP6 transcription at the onset of nodule senescence in Medicago truncatula.","authors":"Li Yang, Lisa Frances, Fernanda de Carvalho-Niebel, Pierre Frendo, Eric Boncompagni","doi":"10.1093/pcp/pcaf110","DOIUrl":"https://doi.org/10.1093/pcp/pcaf110","url":null,"abstract":"The symbiotic association of legumes with rhizobia results in the formation of new root organs called nodules. However, the lifespan of nodules is limited by the senescence process. Increased proteolytic activity is one of the hallmarks of nodule senescence. In Medicago truncatula, a papain cysteine protease encoding gene, MtCP6, is a marker for the onset of nodule senescence under both developmental and stress-induced pathways. To identify the promoter regions responsible for the senescence-related expression of MtCP6, progressive MtCP6 promoter deletions were generated and fused with the GUS reporter for promoter::GUS activity analysis in transgenic M. truncatula roots. In planta, a minimal promoter sequence of 67 bp was identified as sufficient for specific spatiotemporal transcriptional activation of MtCP6 in nodules. The functionality of this promoter regulatory module, thereafter named 'nodule senescence (NS) promoter regulatory module', was validated by both gain- and loss-of-function approaches in M. truncatula. A yeast-one-hybrid (Y1H) screen identified the AP2/ERF transcription factor ERF091, shown to positively regulate nodulation in Lotus japonicus, as an NS- interacting factor. Further Y1H and Nicotiana transactivation assays demonstrated the specificity of ERF91 to interact with and mediate transcription activation of the NS promoter regulatory motif. This work has uncovered a new senescence-related nodule-specific regulatory region and provides evidence for the likely involvement of a stress-related ERF family member in the regulation of MtCP6, at the onset of nodule senescence.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145125764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photochemical reflectance index and its relation to photosynthetic characteristics under dynamic light environment. 动态光环境下光化学反射率及其与光合特性的关系

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-09-11 DOI: 10.1093/pcp/pcaf111

Jing-Qi Zhang, Kouki Hikosaka, Hajime Tomimatsu

{"title":"Photochemical reflectance index and its relation to photosynthetic characteristics under dynamic light environment.","authors":"Jing-Qi Zhang, Kouki Hikosaka, Hajime Tomimatsu","doi":"10.1093/pcp/pcaf111","DOIUrl":"https://doi.org/10.1093/pcp/pcaf111","url":null,"abstract":"The photochemical reflectance index (PRI) is a normalized reflectance index that is expected to be useful for estimating photosynthetic activity based on remote-sensing images. Experimental and theoretical studies have examined how the PRI is related to photosynthesis, but they have been based on observations under steady-state light conditions. Photosynthetic systems display differential temporal responsiveness when exposed to variation in light intensity. Here, we examined the responses of the CO2 assimilation rate (A), quantum yield of PSII photochemistry (ФP), nonphotochemical quenching (NPQ), and the PRI in two poplar species, one being a hybrid that does not close stomata in the dark (nonclosing type). When dark-adapted leaves were exposed to strong light (induction phase), the response time was ФP = NPQ < PRI < A for the normal type and ФP = NPQ < PRI = A for the nonclosing type. Consequently, the PRI-NPQ and the PRI-A relationships differed between the steady-state and induction phase. On the other hand, when the light-adapted leaves were transferred from dark to light, the time response was similar among ФP, NPQ, and the PRI. Therefore, the PRI can be used to assess ФP and NPQ even under dynamic light conditions if light-adapted leaves are used. Our results imply that, following sudden increases in light intensity, CO2 assimilation in the normal type poplar is limited by stomatal conductance, and that PSII-related parameters, including the PRI, are temporally decoupled from A. Estimates of A based on the PRI would be overestimates under dynamic conditions, which needs to be taken into account when interpreting remote-sensing data.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145192520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multifaceted roles of rice ABA/stress-induced intrinsically disordered proteins in augmenting drought resistance. 水稻ABA/胁迫诱导的内在无序蛋白在增强抗旱性中的多重作用。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-09-10 DOI: 10.1093/pcp/pcaf112

Meng-Chun Lin, I-Chieh Tseng, Ching-Lan Wang, Wen-Rong Hsiao, Yun-Jhih Shih, Wan-Chi Lin, Wen-Dar Lin, Su-May Yu, Tuan-Hua David Ho

{"title":"Multifaceted roles of rice ABA/stress-induced intrinsically disordered proteins in augmenting drought resistance.","authors":"Meng-Chun Lin, I-Chieh Tseng, Ching-Lan Wang, Wen-Rong Hsiao, Yun-Jhih Shih, Wan-Chi Lin, Wen-Dar Lin, Su-May Yu, Tuan-Hua David Ho","doi":"10.1093/pcp/pcaf112","DOIUrl":"https://doi.org/10.1093/pcp/pcaf112","url":null,"abstract":"Water deficit stress causes devastating loss of crop yield worldwide. Improving crop drought resistance has become an urgent issue. Here we report that a group of abscisic acid (ABA)/drought stress-induced monocot-specific, intrinsically disordered, and highly proline-rich proteins, REPETITIVE PROLINE-RICH PROTEINS (RePRPs), play pivotal roles in drought resistance in rice seedlings. Rice ectopically expressing RePRPs outlive wild-type rice under extreme drought conditions primarily due to two underlying mechanisms. First, RePRP reduces water loss by decreasing stomata conductance in shoot and enhances levels of extracellular water barriers such as lignin and suberin, primarily in the root vascular bundle. Genes involved in lignin biosynthesis, especially the wall-bound peroxidase responsible for the final assembly of the lignin network, were induced by RePRP. Second, overexpression of RePRP leads to lowered root osmotic potential. Root cell osmotic pressure is more negative in rice plants overexpressing RePRP2 than wild-type plants, and the concentration of a key osmolyte, proline, is enhanced. Hence, ABA/stress-induced RePRP expression leads to several beneficial traits of drought resistance, including lower water loss upon dehydration and higher root water use efficiency under drought conditions. These unique stress proteins may be an important target for technology development in enhancing drought stress resistance in cereals.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145030374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation, structural characterization, and ultrafast energy transfer dynamics of the phycobilisome-photosystem II megacomplex in a thermophilic cyanobacterium. 嗜热蓝藻中藻胆体-光系统II巨型复合物的制备、结构表征和超快能量传递动力学。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-09-10 DOI: 10.1093/pcp/pcaf076

Keisuke Kawakami, Miki Bandou-Uotani, Masatoshi Kida, Yoshihiro Kato, Yuma Hirota, Yuu Hirose, Daisuke Kosumi, Koji Yonekura

{"title":"Preparation, structural characterization, and ultrafast energy transfer dynamics of the phycobilisome-photosystem II megacomplex in a thermophilic cyanobacterium.","authors":"Keisuke Kawakami, Miki Bandou-Uotani, Masatoshi Kida, Yoshihiro Kato, Yuma Hirota, Yuu Hirose, Daisuke Kosumi, Koji Yonekura","doi":"10.1093/pcp/pcaf076","DOIUrl":"https://doi.org/10.1093/pcp/pcaf076","url":null,"abstract":"Phycobilisome (PBS) is a water-soluble light-harvesting supercomplex found in cyanobacteria, glaucophytes, and rhodophytes. PBS interacts with photosynthetic reaction centers, specifically photosystems II and I (PSII and PSI), embedded in the thylakoid membrane. It is widely accepted that PBS predominantly associates with PSII, which functions as the initial complex in the linear electron transport chain. Structures of various types of PBSs with different morphologies and/or absorption properties have been reported using cryo-electron microscopy and X-ray crystallography. However, the detailed energy transfer process between PBS and PSII remains to be elucidated due to the lack of a reliable preparation method for PBS-PSII megacomplexes, in which PBS and PSII interact with each other. In this study, we established a new method for isolating the PBS-PSII megacomplex using ammonium sulfate and dodecyl-α-D-maltoside as a stabilizing reagent and a detergent, respectively. In addition, we evaluated the detailed energy transfer mechanism in the PBS-PSII megacomplex, revealing the rate constants of the funnel-type excitation energy transfer from PBS to PSII. The method will enhance our understanding of the biochemical properties and energy transfer dynamics of diverse PBS-PSII megacomplexes.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145030455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CsWRKY15 from tea plant promotes its auto-resistance when intercropped with chestnut. 茶树CsWRKY15与板栗间作可提高其自抗能力。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-09-06 DOI: 10.1093/pcp/pcaf106

Yan Bai, Meng Li, Qingfen Wang, Yuan Gao, Longfeng Yu, Yanhong Liu, Tian Wu

{"title":"CsWRKY15 from tea plant promotes its auto-resistance when intercropped with chestnut.","authors":"Yan Bai, Meng Li, Qingfen Wang, Yuan Gao, Longfeng Yu, Yanhong Liu, Tian Wu","doi":"10.1093/pcp/pcaf106","DOIUrl":"https://doi.org/10.1093/pcp/pcaf106","url":null,"abstract":"To explore the role of WRKY transcription factors in resistance, a WRKY15 homologous gene, CsWRKY15, and its promoter were isolated from tea plants when intercropped with chestnut. CsWRKY15 expression was significantly induced by ethephon, polyethylene glycol (PEG), and low temperature. Notably, its expression was strongly induced by exogenous gibberellic acid (GA3). A CsWRKY15 overexpression vector was constructed and transformed into tobacco plants. Overexpressing CsWRKY15 enhanced biotic and abiotic stress tolerance in transgenic tobacco plants by increasing antioxidant enzyme activity, upregulating stress-related genes, and activating GA signaling pathway-related genes. The transgenic tobacco plants also showed improved biotic and abiotic stress resistance through enhanced physiological indicators and strengthened physical barriers. Moreover, the homeodomain-leucine zipper protein HAT5 from tea plants (CsHAT5) bound to the LTR (low temperature responsive element), GARE (GA-responsive element), and MBS (MYB binding site) elements in the CsWRKY15 promoter to activate its expression. These results indicated that CsWRKY15 play a positive regulatory role in both biotic and abiotic stress responses. Overall, enhanced GA biosynthesis and signaling activated CsWRKY15 expression, which in turn upregulated antioxidant enzymes and defense-related genes, promoting auto-resistance in tea plants when intercropped with chestnut and improving tea plants health.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145006521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NMOD: A Multi-Dimensional Omics Database for Disease Resistance Research in Nicotiana. NMOD：烟草抗病研究的多维组学数据库。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-09-03 DOI: 10.1093/pcp/pcaf101

He Xu, Yanru Song, Bin Li, Kangkang Song, Xiaohua Zhang, Wenna Zheng, Bin Tan, Long Yang

{"title":"NMOD: A Multi-Dimensional Omics Database for Disease Resistance Research in Nicotiana.","authors":"He Xu, Yanru Song, Bin Li, Kangkang Song, Xiaohua Zhang, Wenna Zheng, Bin Tan, Long Yang","doi":"10.1093/pcp/pcaf101","DOIUrl":"https://doi.org/10.1093/pcp/pcaf101","url":null,"abstract":"High-throughput sequencing has generated extensive omics data for Nicotiana species, a key model genus in the Solanaceae family. However, fragmented data and limited cross-species integration in current databases hinder the identification of disease-resistant genes and germplasm innovation. To address these challenges, we developed Nicotiana Multi-Dimensional Omics Database (http://biodb.com.cn/NMOD/index.html). This database systematically integrates whole-genome data from 23 tobacco varieties, 168 transcriptome datasets, 777 million variation sites, and phenotypic-agronomic data from 146 global germplasm accessions. NMOD emphasizes the annotation of 29 disease-resistant gene families across 10 representative varieties, performs differential expression analysis on transcriptomes under different disease resistance treatments and integrates tools for genomic visualization (JBrowse), homology searching (BLAST), and functional enrichment analysis. In summary, NMOD provides extensive insights into tobacco genomics and genetics, holding promise to enhance future research on disease resistance mechanisms and molecular breeding in tobacco.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic Dynamics of Petal Development in the One-day Flower Species, Japanese Morning Glory (Ipomoea nil). 日花品种日本牵牛花（Ipomoea nil）花瓣发育的转录组动力学

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-09-03 DOI: 10.1093/pcp/pcaf108

Soya Nakagawa, Atsushi Hoshino, Kazuyo Ito, Hiroyo Nishide, Katsuhiro Shiratake, Atsushi J Nagano, Yasubumi Sakakibara

{"title":"Transcriptomic Dynamics of Petal Development in the One-day Flower Species, Japanese Morning Glory (Ipomoea nil).","authors":"Soya Nakagawa, Atsushi Hoshino, Kazuyo Ito, Hiroyo Nishide, Katsuhiro Shiratake, Atsushi J Nagano, Yasubumi Sakakibara","doi":"10.1093/pcp/pcaf108","DOIUrl":"https://doi.org/10.1093/pcp/pcaf108","url":null,"abstract":"Various aspects of Japanese morning glory (Ipomoea nil) petals, such as color, pattern, shape, flower opening time, and senescence, have been extensively studied. To facilitate such studies, transcriptome data were collected from flower petals at 3-h intervals over 3.5 days; the data was collected 72 h before and 12 h post-flower opening, accounting for 29 timepoints. This dataset serves as a comprehensive foundation for analyzing transcriptomic dynamics across a wide spectrum of developmental stages, ranging from closed buds to fully opened flowers and subsequently senescing petals. Gene ontology analysis highlighted substantial transcriptomic changes between the pre-flowering and post-flowering stages. The short-interval sampling facilitated the identification of 805 genes exhibiting circadian rhythmicity. Further transcriptome analysis provided insights into petal development, senescence, and coloration. The expression patterns of cell division marker genes indicated that cell division practically stops at approximately 48 h before the flower opens. Furthermore, the increased expression of genes encoding transporters for sugars, amino acids, nucleic acids, and autophagy-related genes was observed post-flower opening, indicating the translocation of nutrients from senescing petal cells to other developing tissues. Correlations were identified between the temporal expression patterns of three transcriptional regulators and distinct sets of structural genes within the anthocyanin biosynthesis pathway. These findings suggest that each regulator plays a unique role in activating specific structural genes. The temporal transcriptome data and interactive database (https://ipomoeanil.nibb.ac.jp/fpkm/) offer valuable insights into gene expression dynamics, periodicity, and correlations and provide a crucial resource for further research on I. nil and other plant species.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bigger is Better; Modern Cannabis Trichomes are Larger and More Productive than their Landrace Ancestors. 越大越好；现代大麻毛状体比它们的本土祖先更大，更多产。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-09-02 DOI: 10.1093/pcp/pcaf105

Matthew Nolan, Qi Guo, Lennard Garcia-de Heer, Lei Liu, Nicolas Dimopoulos, Bronwyn J Barkla, Tobias Kretzschmar

{"title":"Bigger is Better; Modern Cannabis Trichomes are Larger and More Productive than their Landrace Ancestors.","authors":"Matthew Nolan, Qi Guo, Lennard Garcia-de Heer, Lei Liu, Nicolas Dimopoulos, Bronwyn J Barkla, Tobias Kretzschmar","doi":"10.1093/pcp/pcaf105","DOIUrl":"https://doi.org/10.1093/pcp/pcaf105","url":null,"abstract":"Cannabis sativa L. (Cannabis) is a medicinal plant that produces and stores an abundance of therapeutic and psychoactive secondary metabolites, including phytocannabinoids and terpenes, in the glandular trichomes of its female flowers. We postulate that glandular trichome productivity has been under strong artificial selection in the pursuit for ever more potent cultivars. By comparing glandular trichomes of two modern cultivars and two traditional landraces, contrasting for Cannabidiol (CBD) and Tetrahydrocannabinol (THC) contents, this study aims to identify drivers of enhanced phytocannabinoid productivity in improved drug cultivars. Fluorescent light microscopy, targeted metabolite analysis, and quantitative proteomics were used to examine differences in trichome morphology and metabolic activity. The increased concentrations of phytocannabinoids and terpenes of modern cannabis cultivars were reflected in larger trichomes, that contained more secretory cells compared to traditional landraces. Proteomic analysis indicated that these modern trichome phenotypes were supported by increased metabolic activity, particularly in pathways related to energy production and lipid metabolism. Weighted Gene Co-expression Network Analysis (WGCNA) suggested that histone H2A (H2AXA) involved in DNA repair, Regulator of Fatty-acid Compostion3 (RFC3) involved in non-photosynthetic plastid development and olivetolic acid cyclase (OAC) involved in phytocannabinoid biosynthesis are central hub proteins associated with high Tetrahydrocannabinolic acid (THCA) production. This study highlights the morphological and molecular differences observed between the specific modern and traditional Cannabis cultivars analysed in this study, offering valuable insights for enhancing phytocannabinoid production through targeted breeding and biotechnological approaches.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolism of L-threonate, an ascorbate degradation product, requires a protein with L-threonate metabolizing domains in Arabidopsis. l -苏酸是一种抗坏血酸降解产物，其代谢需要一种具有l -苏酸代谢结构域的蛋白质。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-09-02 DOI: 10.1093/pcp/pcaf104

Kojiro Yamamoto, Yukino Yamashita, Tamami Hamada, Atsuko Miyagi, Hideki Murayama, Akane Hamada, Takanori Maruta

{"title":"Metabolism of L-threonate, an ascorbate degradation product, requires a protein with L-threonate metabolizing domains in Arabidopsis.","authors":"Kojiro Yamamoto, Yukino Yamashita, Tamami Hamada, Atsuko Miyagi, Hideki Murayama, Akane Hamada, Takanori Maruta","doi":"10.1093/pcp/pcaf104","DOIUrl":"https://doi.org/10.1093/pcp/pcaf104","url":null,"abstract":"L-Threonate is one of the major degradation products of ascorbate in plants. While bacteria can utilize L-threonate as a sole carbon source by converting it to dihydroxyacetone phosphate, a glycolysis intermediate, through a three- or four-step metabolic pathway, the corresponding processes in plants remain uncharacterized. Remarkably, an Arabidopsis gene encodes a unique protein containing domains homologous to all three enzymes involved in the bacterial three-step pathway. We designated this protein as L-threonate metabolizing domains (LTD) and investigated its functional role in plant L-threonate metabolism. Despite extensive efforts, recombinant expression of LTD was unsuccessful, likely due to its large protein size. Therefore, a reverse genetic approach was employed, using ltd knockout Arabidopsis lines to explore LTD function. Under continuous dark conditions, where ascorbate degradation is facilitated, LTD transcription was significantly upregulated, leading to increased L-threonate dehydrogenase activity. Knockout lines of LTD exhibited no detectable L-threonate dehydrogenase activity under both light and dark conditions, alongside elevated levels of L-threonate compared to wild-type plants. Although it remains to be determined whether LTD itself possesses all the enzymatic activities required for the bacterial three-step pathway, these results indicate that LTD is essential for L-threonate metabolism in Arabidopsis. The LTD gene is highly conserved among land plants but is absent in green algae, providing a hypothesis that the rise in ascorbate concentrations during plant evolution necessitated a more active metabolism of ascorbate degradation products.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0