NMOD: A Multi-Dimensional Omics Database for Disease Resistance Research in Nicotiana.

Abstract

High-throughput sequencing has generated extensive omics data for Nicotiana species, a key model genus in the Solanaceae family. However, fragmented data and limited cross-species integration in current databases hinder the identification of disease-resistant genes and germplasm innovation. To address these challenges, we developed Nicotiana Multi-Dimensional Omics Database (http://biodb.com.cn/NMOD/index.html). This database systematically integrates whole-genome data from 23 tobacco varieties, 168 transcriptome datasets, 777 million variation sites, and phenotypic-agronomic data from 146 global germplasm accessions. NMOD emphasizes the annotation of 29 disease-resistant gene families across 10 representative varieties, performs differential expression analysis on transcriptomes under different disease resistance treatments and integrates tools for genomic visualization (JBrowse), homology searching (BLAST), and functional enrichment analysis. In summary, NMOD provides extensive insights into tobacco genomics and genetics, holding promise to enhance future research on disease resistance mechanisms and molecular breeding in tobacco.