Plant and Cell Physiology最新文献_第6页

Current perspectives on proton and electron transfer pathways in photosystem II water oxidation. 光系统II水氧化过程中质子和电子转移途径的研究进展。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-08-30 DOI: 10.1093/pcp/pcaf107

Hiroshi Ishikita, Keisuke Saito

{"title":"Current perspectives on proton and electron transfer pathways in photosystem II water oxidation.","authors":"Hiroshi Ishikita, Keisuke Saito","doi":"10.1093/pcp/pcaf107","DOIUrl":"https://doi.org/10.1093/pcp/pcaf107","url":null,"abstract":"Photosystem II (PSII) catalyzes the light-driven oxidation of water, progressing via sequential oxidation states (S-states) of the Mn4CaO5 cluster. Among structural snapshots of intermediate S-states obtained using X-ray free-electron laser (XFEL) crystallography, two-flash XFEL structures assigned to the S3 state reveal an additional oxygen atom (O6) near the O5 site of the cluster, leading to proposals that O6 is incorporated as a new substrate water molecule during the S2 to S3 transition. However, recent re-analyses of the XFEL data highlight potential complications, including conformational heterogeneity, refinement bias, and possible radiation-induced artifacts. In addition, many proposals have been put forwarded without evaluating associated proton and electron transfer processes, despite the fact that water oxidation involves the stepwise removal of protons and electrons. Here, we shed light on electron and proton transfer events during the photocycle by summarizing mechanistic proposals, including those in which O6 is not incorporated. If the remaining reduced site, Mn1(III), is oxidized during the S2 to S3 transition, this step encounters difficulties due to its high redox potential and poor electronic coupling with the electron acceptor, D1-Tyr161 (TyrZ). Efficient proton transfer requires pre-existing H-bond networks, which are absent near O5 and O6, imposing kinetic penalties on proton release. Assigning O6 as a substrate oxygen would imply that O5 is the other substrate, requiring its deprotonation earlier in the Kok cycle.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Autophagic cell death in plants. 植物细胞自噬性死亡。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-08-30 DOI: 10.1093/pcp/pcaf103

Md Arif Sakil, Prodipto Bishnu Angon, Mohammed Arif Sadik Polash, Sujit Mondal, Meskul Zannat, Md Shafiul Islam, Takuya Norizuki, Takashi Ueda

引用次数: 0

Evolution and Regulatory Diversification of Plastid F1FO-ATP Synthase. 质体F1FO-ATP合酶的进化与调控多样化。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-08-29 DOI: 10.1093/pcp/pcaf100

Kaori Kohzuma, Sota Muraoka, Minoru Kumazawa, Kentaro Ifuku

{"title":"Evolution and Regulatory Diversification of Plastid F1FO-ATP Synthase.","authors":"Kaori Kohzuma, Sota Muraoka, Minoru Kumazawa, Kentaro Ifuku","doi":"10.1093/pcp/pcaf100","DOIUrl":"https://doi.org/10.1093/pcp/pcaf100","url":null,"abstract":"F1FO-ATP synthase, the enzyme complex responsible for ATP production, is universally conserved and central to cellular energy metabolism in bacteria as well as in mitochondria and plastids - organelles derived from ancestral bacteria. Although its basic structure and rotational catalytic mechanism are conserved, F1FO-ATP synthase exhibits remarkable regulatory diversity, which is evident in its structural variations, tissue-specific isoforms, and ATP synthesis and hydrolysis mechanisms, reflecting the metabolic demands and environmental contexts of different organisms and organelles. Among the diverse F1FO-ATP synthase isoforms, the plastid F1FO-ATP synthase exhibits unique regulatory features, including redox-dependent modulation, which adjusts enzyme activity in response to light availability. Certain angiosperms possess two isoforms of the γ subunit, encoded by ATPC1 and ATPC2, which give rise to redox-sensitive and redox-insensitive forms of the enzyme, respectively. The latter is active in the dark and may contribute to the maintenance of the proton motive force regulation, thereby supporting stress adaptation in non-photosynthetic tissues. In this review, through a phylogenetic analysis of the γ subunit, we integrate structural, physiological, and evolutionary aspects of plastid F1FO-ATP synthase and discuss how the diversification of ATP synthases, especially within plastid, underpins their broader physiological significance beyond ATP production. Furthermore, we discuss why the chloroplast ATP synthase must be redox-regulated.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ABA Receptor Isoforms Differently Regulate Stomatal Movements and Generation of Reactive Oxygen Species in ABA Signaling in Arabidopsis Guard Cells. ABA受体异构体对拟南芥保护细胞ABA信号中气孔运动和活性氧产生的不同调控

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-08-28 DOI: 10.1093/pcp/pcaf102

Ye Yin, Yuki Hayashi, Monira Sirajam, Shintaro Munemasa, Yoshimasa Nakamura, Toshinori Kinoshita, Yoshiyuki Murata, Izumi C Mori

{"title":"ABA Receptor Isoforms Differently Regulate Stomatal Movements and Generation of Reactive Oxygen Species in ABA Signaling in Arabidopsis Guard Cells.","authors":"Ye Yin, Yuki Hayashi, Monira Sirajam, Shintaro Munemasa, Yoshimasa Nakamura, Toshinori Kinoshita, Yoshiyuki Murata, Izumi C Mori","doi":"10.1093/pcp/pcaf102","DOIUrl":"https://doi.org/10.1093/pcp/pcaf102","url":null,"abstract":"Abscisic acid (ABA) signaling in stomatal guard cells is crucial for plants to cope with abiotic stress condition. Pyrabactin is a synthetic agonist of ABA that has a selective affinity to limited isoforms of ABA receptors. Here we investigated the differential utilization of downstream signaling events in guard cell ABA signaling under specific receptor isoforms taking advantage of pyrabactin affinity. Pyrabactin induced stomatal closure as well as ABA, while it did not inhibit stomatal opening. Plasma membrane inwardly rectifying K+ channel was not regulated by pyrabactin, while H+-ATPase activation was negatively regulated by pyrabactin. Pharmacological and molecular genetic evidence supported that reactive oxygen species production occurred differentially between the closure-inducing and opening-inhibiting signals in guard cells. These findings offered a previously unidentified mechanism for ABA signaling events promoting closure induction and opening inhibition of stomata, which were distinct from each other and governed by different ABA receptor isoforms discriminable by their affinity for pyrabactin.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thylakostasis: Key Factors in Thylakoid Membrane Organization with Emphasis on Biogenesis and Remodeling Proteins in Vascular Plants. 囊体平衡：维管植物类囊体膜组织的关键因素——以生物发生和重塑蛋白为重点。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-08-22 DOI: 10.1093/pcp/pcaf098

Wataru Sakamoto

{"title":"Thylakostasis: Key Factors in Thylakoid Membrane Organization with Emphasis on Biogenesis and Remodeling Proteins in Vascular Plants.","authors":"Wataru Sakamoto","doi":"10.1093/pcp/pcaf098","DOIUrl":"https://doi.org/10.1093/pcp/pcaf098","url":null,"abstract":"The thylakoid membrane (TM), a defining feature for almost all oxygen-evolving photosynthetic organisms, serves as the structural foundation for light-driven energy conversion. In vascular plants, the TM evolved into a complex architecture composed of single-layered stroma thylakoids and stacked grana thylakoids, enabling the spatial organization of two photosystems (PSII and PSI) to optimize light capture and energy transfer. In addition, two membrane regions, one connecting these two compartments (grana margin) and the other corresponding to the curvature domain in grana, function in dissipating excess energy, balancing electron transfer, and maintaining functional PSII. Recent advances in electron microscopy imaging and proteome analysis of membrane subcompartments have provided new insights into the structure and dynamic adaptations of the TM in response to diverse environmental conditions. To describe the mechanisms that govern TM architecture, dynamics and integrity, I introduce the concept of 'thylakostasis' (thylakoid homeostasis). Here, I provide an overview of the molecular components and processes central to thylakostasis, including the biosynthesis of lipids, chlorophyll, and proteins. I focus particularly on the membrane remodeling proteins whose functions have been elucidated recently, such as VIPP1, a member of the evolutionarily conserved PspA/ESCRT-III superfamily; FZL, a dynamin-like GTPase; and CURT1, a curvature-inducing protein unique to photosynthetic organisms. Together, these factors orchestrate TM biogenesis, remodeling, and adaptive flexibility essential for photosynthetic efficiency.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional conservation of the non-canonical strigolactone biosynthetic pathway in root parasitic plants. 根寄生植物非规范独脚金内酯生物合成途径的功能保护。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-08-22 DOI: 10.1093/pcp/pcaf099

Daichi Okawa, Takumi Yuge, Mayu Kawabuchi, Shiho Jitsukawa, Masahiko Otani, Risa Fujita, Michio Kuruma, Kenji Miura, Satoko Yoshida, Kotaro Nishiyama, Yoshinori Fukasawa, Marco Bürger, Takahito Nomura, Yoshiya Seto

{"title":"Functional conservation of the non-canonical strigolactone biosynthetic pathway in root parasitic plants.","authors":"Daichi Okawa, Takumi Yuge, Mayu Kawabuchi, Shiho Jitsukawa, Masahiko Otani, Risa Fujita, Michio Kuruma, Kenji Miura, Satoko Yoshida, Kotaro Nishiyama, Yoshinori Fukasawa, Marco Bürger, Takahito Nomura, Yoshiya Seto","doi":"10.1093/pcp/pcaf099","DOIUrl":"https://doi.org/10.1093/pcp/pcaf099","url":null,"abstract":"Root parasitic plants in the Orobanchaceae family cause widespread and serious damage to crop production, especially in sub-Saharan Africa. The obligate root parasitic plants in this family, such as Striga and Orobanche, germinate in response to host root-derived small molecules called strigolactones (SLs). Facultative root parasitic plants in the same family, such as Phtheirospermum japonicum, do not require SLs for germination. However, they also sense host root-derived SLs for the chemotropic response to grow toward the host. Thus, both the obligate and facultative root parasitic plants can detect the presence of the host by sensing host-derived SLs. Whether root parasitic plants themselves can biosynthesize SLs has not been resolved. To address this fundamental question, we searched for SL biosynthetic genes in both obligate (Orobanche minor) and facultative (Phtheirospermum japonicum) parasitic plants and analyzed the biochemical function of those gene products. Our results demonstrate that the biochemical functions of SL biosynthetic enzymes (D27, CCD7, CDD8, CYP711A, CLAMT) in both plant species were conserved compared with the corresponding orthologs in non-parasitic plant species. Therefore, our results strongly suggest that the root parasitic plants can themselves produce SLs.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamics of full-length Arabidopsis myosin XI and its involvement in actin Remodeling. 全长拟南芥myosin XI的动态及其参与肌动蛋白重塑。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-08-21 DOI: 10.1093/pcp/pcaf097

Jun Obara, Motoki Tominaga

{"title":"Dynamics of full-length Arabidopsis myosin XI and its involvement in actin Remodeling.","authors":"Jun Obara, Motoki Tominaga","doi":"10.1093/pcp/pcaf097","DOIUrl":"https://doi.org/10.1093/pcp/pcaf097","url":null,"abstract":"Plant myosin XI plays a crucial role in intracellular transport, known as cytoplasmic streaming. Previous studies have identified associations between myosin XI and organelles by using a cargo-binding tail domain that lacks a motor domain. However, the subcellular localization and dynamics of full-length myosin XI remain poorly understood. Here, we report the imaging of GFP-fused full-length Arabidopsis myosin XI by transient expression in Nicotiana benthamiana leaves. Live-cell imaging revealed that the localization of several members of full-length Arabidopsis myosin XI differed from that of their respective tail domains. Of these myosin XI members, we focused on myosin XI-2, a major driving force of cytoplasmic streaming. Full-length myosin XI-2 localized to dot-like structures moving along actin filaments. Interestingly, these structures frequently accumulated and dissociated at actin filament intersections, indicating unique, previously unreported intracellular dynamics driven by myosin XI. This behavior was not observed by the expression of the myosin XI-2 tail domain or the ΔGTD mutant, which lacks actin-binding and cargo-binding abilities, respectively. High temporal resolution imaging with simultaneous dual-color excitation revealed that accumulation structures gradually shifted their position in conjunction with active actin filament translocation. Moreover, active actin array dynamics were observed in both full-length myosin XI-2 expressing and non-expressing cells, whereas these dynamics were suppressed in myosin XI-2-tail expressing cells. Collectively, these results suggest that myosin XI-2 regulates actin array dynamics through interactions with both actin filaments and cargo.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The rice BRITTLE CULM 4 gene encodes a membrane protein involved in cellulose synthesis in the secondary cell wall. 水稻脆性culm4基因编码一种参与次级细胞壁纤维素合成的膜蛋白。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-08-21 DOI: 10.1093/pcp/pcaf096

Masatoshi Yamaguchi, Ami Sato, Daisuke Takahashi, Kazuhisa Mori, Ryota Fujimoto, Atsuko Miyagi, Eriko Sato, Toshiki Ishikawa, Ryosuke Sano, Tetsuya Kurata, Shiro Suzuki, Yasuko Kaneko, Maki Kawai-Yamada, Toshihisa Kotake

{"title":"The rice BRITTLE CULM 4 gene encodes a membrane protein involved in cellulose synthesis in the secondary cell wall.","authors":"Masatoshi Yamaguchi, Ami Sato, Daisuke Takahashi, Kazuhisa Mori, Ryota Fujimoto, Atsuko Miyagi, Eriko Sato, Toshiki Ishikawa, Ryosuke Sano, Tetsuya Kurata, Shiro Suzuki, Yasuko Kaneko, Maki Kawai-Yamada, Toshihisa Kotake","doi":"10.1093/pcp/pcaf096","DOIUrl":"https://doi.org/10.1093/pcp/pcaf096","url":null,"abstract":"The formation of secondary cell walls, which provide mechanical strength to the plant body, depends on numerous factors. Studies on rice brittle culm (bc) mutants allow us to identify these factors and gain insights into the mechanisms of secondary cell wall formation. Rice bc4 is a recessive bc mutant with fragile culms and leaves, similar to other bc mutants. We found that the bc4 mutant exhibited reduced cellulose content in the culm cell walls compared to the japonica cultivar Taichung 65 and the indica cultivar Kasalath, while hemicellulose content remained unchanged. Transmission electron microscopy revealed reduced cell wall thickness in the sclerenchyma cells of the bc4 culm, indicating that BC4 contributes to normal cellulose synthesis or deposition in secondary cell walls. Positional cloning and subsequent genome sequencing revealed that the BC4 gene encodes a four α-helical transmembrane protein with 205 amino acids, and that the bc4 mutation results in a premature termination codon in this gene. Four bc4 mutants generated from the japonica cultivar Nipponbare, using genome editing with the CRISPR/Cas9 system, exhibited reduced cellulose content along with bc phenotypes. Gene clustering analysis based on expression patterns and metabolomic analysis suggested that BC4 functions independently from secondary cell wall CesAs and COBRA-like protein. These results suggest that the BC4 protein is a newly identified factor involved in cellulose synthesis or deposition in the secondary cell walls of rice.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Cost of Survival: Mutation in a Barley Strigolactone Repressor HvD53A Impairs Photosynthesis but Increases Drought Tolerance. 生存代价：大麦独角麦内酯抑制因子HvD53A的突变损害了光合作用，但增加了耐旱性。

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-08-12 DOI: 10.1093/pcp/pcaf095

Magdalena Korek, Weronika Buchcik, Beata Chmielewska, Agata Daszkowska-Golec, Irene M Fontana, Michael Melzer, Goetz Hensel, Jochen Kumlehn, Philip B Brewer, Glen R Uhrig, Marek Marzec

{"title":"The Cost of Survival: Mutation in a Barley Strigolactone Repressor HvD53A Impairs Photosynthesis but Increases Drought Tolerance.","authors":"Magdalena Korek, Weronika Buchcik, Beata Chmielewska, Agata Daszkowska-Golec, Irene M Fontana, Michael Melzer, Goetz Hensel, Jochen Kumlehn, Philip B Brewer, Glen R Uhrig, Marek Marzec","doi":"10.1093/pcp/pcaf095","DOIUrl":"https://doi.org/10.1093/pcp/pcaf095","url":null,"abstract":"Strigolactones (SLs) are a class of plant hormones that play a crucial role in shaping plant architecture, significantly influencing plant adaptation to harsh environmental conditions. In this study, we examined the effects of a mutation in a component of the barley SL signaling pathway, the SL repressor HvDWARF53A, on plant growth and drought tolerance. We compared the results with those of a previously described barley mutant, which is highly tillered and drought-sensitive, carrying a mutation in the SL receptor gene HvDWARF14. The two mutants, hvd14.d and hvd53a.f, displayed contrasting phenotypes, including differences in plant height, tillering, and drought sensitivity. Under control conditions, ultrastructural analysis of hvd53a.f revealed smaller chloroplasts and fewer grana stacks, which may account for its reduced photosynthetic efficiency. Conversely, transcriptomic analysis linked the differentially expressed genes in hvd53a.f to antioxidation and stress responses, suggesting a potentially enhanced capacity to cope with drought. Further analysis revealed a strong connection between the SL signaling pathway and circadian clock components. Among these, CIRCADIAN CLOCK ASSOCIATED 1 emerged as a potential SL-responsive transcription factor (TF), possibly playing a key role in regulating tillering. Under drought conditions, hvd53a.f exhibited enhanced tolerance, as evidenced by higher relative water content, reduced chlorophyll degradation, and stable, albeit reduced, photosynthetic performance. Here, we identified the SL-related TF JUNGBRUNNEN 1 as a potential regulator of genes involved in water deficit response and antioxidation processes. Overall, the hvd53a.f mutation enhances drought tolerance while maintaining low, stable photosynthesis, highlighting HvD53A as a central node connecting SL signaling to stress resilience.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144837473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Field dynamics of the root endosphere microbiome assembly in paddy rice cultivated under no fertilizer input. 无施肥条件下水稻根内微生物群组合的田间动态

IF 4 2区生物学

Plant and Cell Physiology Pub Date : 2025-08-12 DOI: 10.1093/pcp/pcaf045

Asahi Adachi, John Jewish Dominguez, Yuniar Devi Utami, Masako Fuji, Sumire Kirita, Shunsuke Imai, Takumi Murakami, Yuichi Hongoh, Rina Shinjo, Takehiro Kamiya, Toru Fujiwara, Kiwamu Minamisawa, Naoaki Ono, Shigehiko Kanaya, Yusuke Saijo

{"title":"Field dynamics of the root endosphere microbiome assembly in paddy rice cultivated under no fertilizer input.","authors":"Asahi Adachi, John Jewish Dominguez, Yuniar Devi Utami, Masako Fuji, Sumire Kirita, Shunsuke Imai, Takumi Murakami, Yuichi Hongoh, Rina Shinjo, Takehiro Kamiya, Toru Fujiwara, Kiwamu Minamisawa, Naoaki Ono, Shigehiko Kanaya, Yusuke Saijo","doi":"10.1093/pcp/pcaf045","DOIUrl":"10.1093/pcp/pcaf045","url":null,"abstract":"Plants accommodate diverse microbial communities, termed the microbiome, which can change dynamically during plant adaptation to varying environmental conditions. However, the direction of these changes and the underlying mechanisms driving them, particularly in crops adapting to the field conditions, are not well understood. Here, we investigate the root endosphere microbiome of rice (Oryza sativa ssp. japonica) across four consecutive cultivation seasons in a high-yield, non-fertilized, and pesticide-free paddy field, compared with a neighboring fertilized and pesticide-treated field. Using 16S rRNA amplicon and metagenome sequencing, we analyzed three Japonica cultivars-Nipponbare, Hinohikari, and Kinmaze. Our findings reveal that the root endosphere microbiomes diverge based on fertilization regime and plant developmental stages, while the effects of cultivar variation are less significant. Machine learning model and metagenomic analysis of nitrogenase (nif) genes suggest enhanced nitrogen fixation activity in the non-fertilized field-grown roots, highlighting a potential role of diazotrophic, iron-reducing bacteria Telmatospirillum. These results provide valuable insights into the assembly of the rice root microbiome in nutrient-poor soil, which can aid in managing microbial homeostasis for sustainable agriculture.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":"1086-1101"},"PeriodicalIF":4.0,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144249355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0