Plant and Cell Physiology最新文献

{"title":"Sieve Element Plastids: An Elusive Organelle.","authors":"Viktoriya V Vasina, Michael Knoblauch","doi":"10.1093/pcp/pcag114","DOIUrl":"https://doi.org/10.1093/pcp/pcag114","url":null,"abstract":"<p><p>Vascular plants use the phloem to transport photoassimilates via sieve elements, which are cells with modified anatomy to accommodate long distance transport. Mature sieve elements lack a majority of cell organelles to reduce friction in the tube system, but some organelles like the endoplasmic reticulum, mitochondria, phloem-proteins and sieve element plastids remain. Most of the functions of sieve element organelles are poorly characterized. This leads to the fact that sieve element plastids are often omitted from textbooks as a plastid type, although they apparently derive from proplastids. As they are ultrastructurally diverse, sieve element plastids have been used for taxonomical purposes, but functional information is scarce. Here we review the currently available literature on the structure and function of sieve element plastids.</p>","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148819318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structurally similar small molecules oppositely regulate circadian timing through distinct mechanisms in Arabidopsis.","authors":"Hiyori Fujikawa, Takahiro N Uehara, Hiromi Matsuo, Saori Takao, Toshinori Kinoshita, Kenichiro Itami, Junichiro Yamaguchi, Norihito Nakamichi","doi":"10.1093/pcp/pcag121","DOIUrl":"https://doi.org/10.1093/pcp/pcag121","url":null,"abstract":"","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148797410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PpXTH5 and PpXTH6, xyloglucan endotransglucosylases, regulate plasmodesmata density and structure in the moss Physcomitrium patens.","authors":"Phu Thanh Ngo, Marcel Pascal Beier, Liang Bao, Mark P Running, Tomomichi Fujita","doi":"10.1093/pcp/pcag113","DOIUrl":"https://doi.org/10.1093/pcp/pcag113","url":null,"abstract":"<p><p>Plant cell-cell communication plays a crucial role during growth and development. While plasmodesmata (PD) are well known in this communication, their biogenesis and regulation remain poorly understood. In this study, we demonstrate that xyloglucan endotransglucosylases (XETs), enzymes involved in transglycosylation of xyloglucan chains, could impact not only cellular growth but also PD development in the moss Physcomitrium patens (P. patens). Knockout mutant lines of two XET isoforms, named Ppxth5, Ppxth6, and Ppxth5,6dko, showed growth reductions in both protonemal and gametophytic stages. Subsequent analyses of these mutants indicated a decrease in PD density, abnormal clusters of xyloglucan and callose, and their colocalization, highlighting their potential involvement in PD formation. Further evaluation of these alterations using Dendra2 photoconvertible protein showed impaired intercellular communication, indicating reduced intercellular transport compared to WT. For PD structural changes, these mutants show higher ratios of incomplete simple PD and complex PD, highlighting the critical role of xyloglucan. Specifically, proper xyloglucan distribution, which is regulated by XET activities, is required for maintenance of PD density and structure, and for intercellular transport, possibly interacting with callose during cell plate formation.</p>","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148765647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intracellular domain of Arabidopsis GENERATIVE CELL SPECIFIC 1/HAPLESS 2 is involved in double fertilization control.","authors":"Ari Yoshimura, Tomoko Igawa","doi":"10.1093/pcp/pcag118","DOIUrl":"https://doi.org/10.1093/pcp/pcag118","url":null,"abstract":"","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148762950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SlSPL10, a Transcription Factor That Suppresses Lateral Bud Outgrowth in Tomato.","authors":"Di Yang, Dengjing Huang, Yandong Yao, Xinfang Chen, Weibiao Liao","doi":"10.1093/pcp/pcag117","DOIUrl":"https://doi.org/10.1093/pcp/pcag117","url":null,"abstract":"<p><p>Lateral bud outgrowth critically determines tomato plant architecture, canopy structure, photosynthate distribution, and fruit yield. SQUAMOSA promoter binding protein-like (SPL) transcription factors regulate multiple developmental processes; however, the role of SlSPL10 in controlling lateral bud outgrowth remains unknown. In this study, we used tomato cultivar Micro-Tom (wild-type, WT), SlSPL10 knockout mutants (slspl10#1 and slspl10#2), and overexpression lines (OE-SlSPL10#6 and OE-SlSPL10#7) to assess agronomic traits and phytohormone levels. The slspl10 mutants exhibited dwarfism, increased lateral bud number, and elongated buds, whereas OE-SlSPL10 lines suppressed bud outgrowth. Phytohormone and sucrose analyses revealed that slspl10 mutants displayed elevated levels of ZT, GA3, and sucrose, but reduced levels of IAA, ABA, and SA, whereas OE-SlSPL10 lines showed opposite trends. To dissect the underlying molecular mechanism, we demonstrated that SlSPL10 directly binds to GTAC motifs in the promoters of SlARF10a and SlABI3 using yeast one-hybrid (Y1H) assays, dual-luciferase reporter (DLR) assays, and chromatin immunoprecipitation-PCR (ChIP-PCR), thereby transactivating both target genes. Virus-induced gene silencing (VIGS) of SlARF10a or SlABI3 phenocopied the slspl10 mutants, showing increased lateral bud number and elongation. Hormone measurements in VIGS lines further indicated that silencing SlARF10a specifically reduced IAA content, whereas silencing SlABI3 specifically reduced ABA content; both lines also exhibited increased ZT, GA3, and sucrose, and decreased SA. Collectively, these results demonstrate that SlSPL10 directly targets SlARF10a and SlABI3 to modulate phytohormone and sucrose homeostasis, thereby precisely inhibiting lateral bud outgrowth in tomato.</p>","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148697853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anther Dehiscence: Mechanisms, Regulation, and Environmental Sensitivity.","authors":"Woo-Taek Jeon, Ahyeon Cheon, Yuree Lee","doi":"10.1093/pcp/pcag115","DOIUrl":"https://doi.org/10.1093/pcp/pcag115","url":null,"abstract":"<p><p>Anther dehiscence is a developmentally programmed, mechanically executed event essential for sexual reproduction in flowering plants. Dehiscence must be synchronized with microspore and pollen maturation, coordinated with filament elongation to ensure correct anther positioning, and remain robust under fluctuating environmental conditions, particularly humidity, which strongly influences dehydration kinetics. At the same time, rapid, sufficiently wide opening requires that spatiotemporal developmental programs be converted into physical forces within a multilayered tissue. Yet despite extensive work on its genetic regulation and physical basis, these dimensions are often treated separately, leaving unresolved how developmental patterning is translated into controlled force generation in the anther wall. Here, focusing primarily on Arabidopsis, we integrate molecular patterning with biomechanics to explain (i) how lineage specification establishes the cellular architecture required for dehiscence; (ii) how hormonal and receptor-kinase signaling synchronize developmental timing with organ-level readiness; and (iii) how dehydration-driven mechanics are regulated and locally executed through epidermal transpiration, cell death, and cell-wall remodeling. We further contrast dehiscence with abscission to highlight a shared logic of spatially patterned reinforcement coupled with focal weakening, and examine how hydration dynamics, tissue mechanics, and geometry shape the timing and extent of opening. Together, these perspectives establish anther dehiscence as a model for understanding how developmental programs are translated into coordinated tissue mechanics and organ-level function.</p>","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148697881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revisiting sex plasticity in Cannabis sativa L.: A hormonal cross-talk in flower masculinisation.","authors":"Ewa Dubas, Monika Krzewska, Iwona Żur, Barbara Dziekońska, Jakub Kurczab, Mateusz Bibro, Piotr Waligórski, Paweł A Rodziewicz","doi":"10.1093/pcp/pcag111","DOIUrl":"https://doi.org/10.1093/pcp/pcag111","url":null,"abstract":"<p><p>Sex determination in Cannabis sativa L. has major implications for breeding, biotechnology, and crop management. In this species, sex is primarily governed by an XX/XY sex chromosome system, with genetic regulation constituting the principal level of control. Variation in sex expression, encompassing dioecious, monoecious, or hermaphroditic forms, may arise from interactions between sex chromosomes and autosomal factors X-to-autosome (X: A) chromosome balance. Secondary modulation of sex phenotype involves environmental and physiological influences, with phytohormonal regulation representing an important layer of sex plasticity. Epigenetic mechanisms may further contribute to the downstream fine-tuning of phenotypic sex expression. Importantly, sex expression in Cannabis can also be manipulated chemically. Several silver-based compounds, such as silver thiosulfate, are widely used to induce staminate flower formation in genetically female plants, whereas application of ethephon promotes female flower formation in genetically male individuals. Although both agents act through ethylene signalling pathway, their effects likely involve broader and more complex interactions within the molecular network, including cross-talk with other hormonal pathways. Within this context, transcriptional regulators associated with auxin signalling, including Aux/IAA - TOPLESS-RELATED co-repressors such as TPR1, may contribute to the activation of male developmental programmes by modulating hormonal response pathways. However, their precise role and position within the sex-regulatory hierarchy are yet to be clarified. The multi-layered regulation of sex determination in Cannabis sativa L. provides a valuable framework for investigating the molecular basis of this process through the integration of genetic, environmental, and hormonal factors. A more comprehensive understanding of these interacting regulatory layers may facilitate the development of strategies for early sex identification, improved control over flower sex conversion, and the optimisation of cannabinoid-rich female inflorescence production. Such advances would support both fundamental and applied approaches in Cannabis biotechnology and cultivation.</p>","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148670666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seeds and Seedlings: How Arabidopsis thaliana integrates light signalling in its earliest life stages.","authors":"David AlDaradkeh, Daphne Ezer, James Ronald","doi":"10.1093/pcp/pcag112","DOIUrl":"https://doi.org/10.1093/pcp/pcag112","url":null,"abstract":"<p><p>The ability to detect and respond to light signals is essential for a plant to maximise its fitness. These signals, including photoperiod, regulate key aspects of a plant's development, from the timing of seed germination through to the floral transition. How the photoperiod regulates hypocotyl elongation and the floral transition is now well understood at the molecular level. In contrast, how light and circadian signals are perceived and integrated in seeds is less clear. In this review, we highlight the latest insights into how light, photoperiod, and circadian signalling regulate development in seedlings and seeds. We also discuss how even though these two developmental stages employ many of the same components, re-wiring of the regulatory network that these components operate within enables the distinct developmental demands of germinating and seedling growth in responding to the environment cues. Finally, we highlight key knowledge gaps and future directions of research in how seedlings and seeds sense integrate light and circadian signals during development.</p>","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148670642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering a Novel Function of the WOX Family: SpWOX1-Mediated Male Suppression in Dioecious Spinach.","authors":"Muhammad Shoaib, Qiangwei Yang, Shuqi Wang, Wenjia Liu, Wanqing Zhang, Qiuying Guo, Ning Tang, Ning Li, Chuanliang Deng","doi":"10.1093/pcp/pcag107","DOIUrl":"https://doi.org/10.1093/pcp/pcag107","url":null,"abstract":"<p><p>Spinach, a dioecious species, serves as an important model for studying sex determination and differentiation in plants. However, the molecular mechanisms underlying these processes remain largely unresolved. The WUSCHEL-related homeobox (WOX) gene family plays critical roles in meristem maintenance, cell fate determination, and plant development, yet remains uncharacterized in spinach. In this study, a genome-wide comparative analysis identified 10-11 WOX genes across the genus Spinacia. Transcriptome analysis across floral developmental stages, combined with weighted gene co-expression network analysis, revealed that SpWOX1 exhibits female-biased expression and is associated with transcription factors involved in floral development. Functional analysis showed that silencing of SpWOX1 using virus-induced gene silencing resulted in the conversion of female flowers into hermaphroditic flowers in spinach, whereas ectopic expression in Arabidopsis thaliana led to reduced pollen viability and impaired stamen development. Furthermore, yeast two-hybrid and bimolecular fluorescence complementation assays demonstrate that SpWOX1 interacts with the masculine factor SpMS1 and three key regulators of floral development, including AFO, AS2 and ARF3. Collectively, these findings suggest that SpWOX1 functions as a suppressor of male organ development and may play a central role in the spinach sex-determination pathway. This study provides new insights into the molecular basis of sex differentiation in spinach.</p>","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148631090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of somatic cell fusion on zygotic development in rice.","authors":"Erika Toda, Takumu Kamekawa, Takeshi Izawa, Takashi Okamoto","doi":"10.1093/pcp/pcag110","DOIUrl":"https://doi.org/10.1093/pcp/pcag110","url":null,"abstract":"<p><p>In angiosperms, a zygote is the initial totipotent cell that develops into an embryo. To reveal whether and how genomic and cytoplasmic contents from different cell types affect zygotic development, we analyzed developmental profiles of zygote-soma hybrid cells produced by rice in vitro fertilization system. When a leaf protoplast (2x) was fused with a zygote (fusion product between egg and sperm cells; 2x), the development of most of the leaf protoplast-fused zygotes with a 1:1 gamete:soma ratio was arrested at the one-cell stage. By contrast, when a leaf protoplast (2x) was fused with a polyploid zygote (3x or 4x), several fractions of the leaf protoplast-fused polyploid zygotes continued to develop. Furthermore, when a leaf protoplast (1x) isolated from a haploid rice plant was used for the fusion, more than half of the haploid leaf protoplast-fused zygotes developed into globular embryo-like structures and cell masses. Several of these calli then regenerated into plantlets, with the expected ploidy levels. Accordingly, leaf and zygote nuclei were integrated into the hybrid nucleus in zygote-soma hybrid cells. The resulting hybrid genome was maintained during the hybrid cell development and regeneration. These findings suggest that developmental profiles of zygote-soma hybrid cells are affected by the gamete:soma ratio. Furthermore, the hybrid cell fate shifts to the zygotic mode when the gamete:soma ratio changes from 1:1 to 2:1.</p>","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148631153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}