Partial photoswitching of rod-shaped phycobilisome production in the cyanobacterium Synechocystis sp. PCC 6803.

IF 4 2区 生物学 Q2 CELL BIOLOGY
Mutsumi Kubushiro, Takuto Otsu, Naomi Misawa, Masako Hamada, Toshihiko Eki, Shigeru Kawai, Yuu Hirose
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Abstract

Certain cyanobacteria can alter the structure of their photosynthetic supercomplex phycobilisome (PBS) in response to changes in ambient light color. This process is known as chromatic acclimation (CA) and is classified into seven types (CA1-CA7), each involving distinct modifications of PBS structure. Among them, CA1 is defined as the regulation of the rod-shaped PBS in response to green and red light, maintaining the excitation balance between the two photosystems. Synechocystis sp. PCC 6803 (PCC 6803) is a widely used model cyanobacterium for photosynthesis research and harbors the CcaSR photosensory gene cluster for CA1. In this study, we investigated the wavelength dependence of the rod-shaped PBS production in PCC 6803. RNA-Seq analysis revealed that the expression of cpcL, which encodes a rod-membrane linker protein of rod-shaped PBS, is upregulated under a wide range of visible light (470-630 nm) and partially suppressed under violet (380-420 nm) and red to far-red light (680-720 nm) conditions. Low-temperature fluorescence emission spectra revealed that the ratio of rod-shaped PBS to hemi-discoidal PBS was highest under green light (530 nm), followed by red (660 nm) and far-red (700 nm) conditions. Furthermore, the isolation of intact PBS fractions using an improved procedure revealed the presence of rod-shaped PBS containing CpcL under these light conditions. The incomplete photoswitching of the rod-shaped PBS production may be due to the adaptation of the photosensory CcaSR system in PCC 6803, which lacks green-absorbing components in its PBSs and provides green-light rich environments in their cell aggregates.

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蓝细菌胞囊藻体产生杆状藻胆酶体的部分光开关。
某些蓝藻可以改变其光合超复合体藻胆酶体(PBS)的结构,以响应环境光颜色的变化。这个过程被称为色驯化(CA),分为七种类型(CA1-CA7),每种类型都涉及PBS结构的不同修饰。其中,CA1被定义为调控杆状PBS对绿光和红光的响应,维持两个光系统之间的激发平衡。Synechocystis sp. PCC 6803 (PCC 6803)是一种被广泛用于光合作用研究的蓝藻模式菌,含有CA1的CcaSR光感基因簇。在这项研究中,我们研究了pcc6803棒状PBS生产的波长依赖性。RNA-Seq分析显示,编码杆状PBS棒膜连接蛋白的cpcL在大范围可见光(470-630 nm)下表达上调,在紫光(380-420 nm)和红光至远红光(680-720 nm)条件下表达部分抑制。低温荧光发射光谱显示,在绿光(530 nm)条件下杆状PBS与半盘状PBS的比例最高,其次是红光(660 nm)和远红光(700 nm)条件。此外,使用改进的程序分离完整的PBS馏分,发现在这些光照条件下存在含有CpcL的棒状PBS。杆状PBS产生的不完全光开关可能是由于PCC 6803中光感CcaSR系统的适应,PCC 6803的PBS中缺乏绿色吸收成分,在其细胞聚集体中提供了丰富的绿光环境。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Plant and Cell Physiology
Plant and Cell Physiology 生物-细胞生物学
CiteScore
8.40
自引率
4.10%
发文量
166
审稿时长
1.7 months
期刊介绍: Plant & Cell Physiology (PCP) was established in 1959 and is the official journal of the Japanese Society of Plant Physiologists (JSPP). The title reflects the journal''s original interest and scope to encompass research not just at the whole-organism level but also at the cellular and subcellular levels. Amongst the broad range of topics covered by this international journal, readers will find the very best original research on plant physiology, biochemistry, cell biology, molecular genetics, epigenetics, biotechnology, bioinformatics and –omics; as well as how plants respond to and interact with their environment (abiotic and biotic factors), and the biology of photosynthetic microorganisms.
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