Plant and Cell Physiology最新文献_第9页

From Pollen Tube Discharge to Pronuclear Union in Arabidopsis: An Evidence-Ranked View of the Terminal Phase of Double Fertilization. 拟南芥从花粉管排出到原核结合：双受精末期的证据排序观点。

IF 4.7 2区生物学

Plant and Cell Physiology Pub Date : 2026-07-22 DOI: 10.1093/pcp/pcag100

Prakash Babu Adhikari, Ryushiro Dora Kasahara

{"title":"From Pollen Tube Discharge to Pronuclear Union in Arabidopsis: An Evidence-Ranked View of the Terminal Phase of Double Fertilization.","authors":"Prakash Babu Adhikari, Ryushiro Dora Kasahara","doi":"10.1093/pcp/pcag100","DOIUrl":"https://doi.org/10.1093/pcp/pcag100","url":null,"abstract":"Double fertilization is still commonly discussed as though its terminal phase were a single problem, 'gamete fusion.' Yet the interval between pollen tube discharge and pronuclear union comprises a sequence of transitions with different evidentiary status. This review narrows the field to that terminal window and evaluates each stage using two criteria: directness of evidence in flowering plants and mechanistic completeness of the internal sequence. Direct observation, in planta perturbation, and comparative or heterologous reasoning are treated as sources of evidence used to support or limit those evaluations. Arabidopsis serves as the primary reference system, with selected angiosperm comparisons included only where they test transferability or illuminate unresolved stages. The main stages considered are post-discharge sperm cell exposure and positioning, attachment and biased engagement, competence acquisition, plasmogamy, post-entry activation and developmental progression, closure and recovery, sperm cell nuclear migration, and pronuclear union. Several stages are now directly distinguished even where their internal mechanism remains incomplete. Flowering plant work has yet to resolve the activating step that converts surface-deployed GCS1/HAP2 into a merger-driving fusogen, the decisive arrest state between stable apposition and full cytoplasmic continuity, the sequence of pore opening, expansion, and post-fusion membrane remodeling, or the order of outer- and inner-nuclear-envelope fusion during pronuclear union. The terminal phase is therefore best understood as a sequence of experimentally distinguishable stages, and robust causal interpretation depends on treating recovery, closure, plasmogamy, and karyogamy as analytically distinct processes.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148562759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Minimum Synthetic Inducible Promoters for Host Response to Parasitic Plants. 宿主对寄生植物响应的最小合成诱导启动子。

IF 4.7 2区生物学

Plant and Cell Physiology Pub Date : 2026-07-20 DOI: 10.1093/pcp/pcag101

Imen Tanniche, Hope Gruszewski, Sukhmanpreet Kaur, James H Westwood

{"title":"Minimum Synthetic Inducible Promoters for Host Response to Parasitic Plants.","authors":"Imen Tanniche, Hope Gruszewski, Sukhmanpreet Kaur, James H Westwood","doi":"10.1093/pcp/pcag101","DOIUrl":"https://doi.org/10.1093/pcp/pcag101","url":null,"abstract":"Parasitic plants are a problem for world agriculture as their direct feeding on hosts can cause high yield losses. One approach to controlling parasitic plants would be to engineer host plants for resistance by expressing an effective defense in response to invasion by the parasite. While much attention has focused on identifying suitable resistance genes, little work has addressed the challenge of identifying promoters to drive specific expression of such genes. To meet this need, we set out to develop a parasite-inducible promoter. Starting with previously generated RNAseq data from Arabidopsis thaliana parasitized by Cuscuta campestris, we identified host genes induced by the parasite and then studied their regulatory regions to identify cis-regulatory elements (CREs). These elements, or transcription factor binding sites (TFBSs), were recombined into a set of minimum synthetic inducible promoters (MSIPs), fused to the β-glucuronidase (GUS) reporter gene, and stably transformed into A. thaliana. The MSIP lines were evaluated with and without Cuscuta, and GUS staining showed that most of the MSIPs were induced by Cuscuta parasitism, although they were also expressed in some non-parasitized tissues. The most parasite-specific MSIP contained stress-inducible TFBSs, and was also responsive to wounding, crushing and jasmonate treatment. Furthermore, A. thaliana parasitized by the root parasite Phelipanche aegyptiaca showed similar MSIP induction patterns in roots, suggesting that at least two types of parasitic plant trigger the same host transcriptional machinery. These MSIPs provide insights into host-parasite interactions and are potentially valuable for driving expression of novel parasite resistance genes.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148520778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide analysis of AP2/ERF genes identifies HpERF144 as a regulator of salt tolerance and phenylpropanoid metabolism in Herpetospermum pedunculosum. AP2/ERF基因的全基因组分析发现HpERF144是带梗Herpetospermum pedduncullosum耐盐性和苯丙素代谢的调节因子。

IF 4.7 2区生物学

Plant and Cell Physiology Pub Date : 2026-07-19 DOI: 10.1093/pcp/pcag103

Rui Li, Daihan Chen, Zhidan Zhu, Xiao Huang, Xinghui Wu, Qin Jiang, Qinyuan Li, Shiyu Yao, Ziwei Zhu, Yixi Yang, Qiuxia Lu, Xu Zhang, Bingliang Liu, Min Sun, Qi Zhao, Yang Tao

{"title":"Genome-wide analysis of AP2/ERF genes identifies HpERF144 as a regulator of salt tolerance and phenylpropanoid metabolism in Herpetospermum pedunculosum.","authors":"Rui Li, Daihan Chen, Zhidan Zhu, Xiao Huang, Xinghui Wu, Qin Jiang, Qinyuan Li, Shiyu Yao, Ziwei Zhu, Yixi Yang, Qiuxia Lu, Xu Zhang, Bingliang Liu, Min Sun, Qi Zhao, Yang Tao","doi":"10.1093/pcp/pcag103","DOIUrl":"https://doi.org/10.1093/pcp/pcag103","url":null,"abstract":"Salt stress disrupts plant physiology and reprograms specialized metabolism. APETALA2/ethylene-responsive factor (AP2/ERF) transcription factors integrate stress and hormone signals, but their roles in linking salt tolerance with phenylpropanoid metabolism in non-model alpine plants remain poorly understood. Here, we performed a genome-wide survey of AP2/ERF genes and functionally characterized a salt-responsive ERF in Herpetospermum pedunculosum. We identified 147 HpAP2/ERF genes, whose promoters were enriched in hormone- and stress-responsive cis-elements. Salt treatment induced widespread expression changes of HpAP2/ERF genes in roots, with HpERF144 among the most rapidly and strongly up-regulated members. Virus-induced silencing and ZYMV-mediated overexpression in H. pedunculosum, together with heterologous overexpression in Nicotiana tabacum, showed that HpERF144 positively regulates salt tolerance by modulating oxidative stress-related physiological responses. Moreover, HpERF144 silencing reduced, whereas overexpression increased, total lignin and total lignan accumulation in roots under salt stress. DAP-seq and dual-luciferase assays further indicated that HpERF144 associates with and activates selected phenylpropanoid- and cell wall-related promoters, including 4CL- and CCR-like targets. These findings identify HpERF144 as a link between salt stress signaling and phenylpropanoid-associated metabolic regulation in a lignan-rich medicinal plant.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148520743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GUN2 and GUN3 Coordinate Heme and Chlorophyll Metabolism Thereby Impacting Singlet Oxygen Production and Photooxidative Responses. GUN2和GUN3协调血红素和叶绿素代谢，从而影响单线态氧气产生和光氧化反应。

IF 4.7 2区生物学

Plant and Cell Physiology Pub Date : 2026-07-19 DOI: 10.1093/pcp/pcag102

Huafan Zhu, Yuhong Li, Wenqiang Yang, Bernhard Grimm, Rongcheng Lin

{"title":"GUN2 and GUN3 Coordinate Heme and Chlorophyll Metabolism Thereby Impacting Singlet Oxygen Production and Photooxidative Responses.","authors":"Huafan Zhu, Yuhong Li, Wenqiang Yang, Bernhard Grimm, Rongcheng Lin","doi":"10.1093/pcp/pcag102","DOIUrl":"https://doi.org/10.1093/pcp/pcag102","url":null,"abstract":"Tetrapyrrole biosynthesis is an absolute essential metabolic pathway in plants that predominantly gives rise to chlorophyll, heme, and phytochromobilin. Dysregulation of tetrapyrrole biosynthesis severely impairs plant growth and development when heme and chlorophyll synthesis are not adjusted to the needs in the respective plant organ and when excessive accumulation of light-absorbing tetrapyrrole intermediates cause oxidative damage. However, how chlorophyll and heme synthesis are properly balanced during early development of greening seedlings remains largely elusive. In this study, we performed a suppressor screen on the photosensitive Arabidopsis thaliana pif1 pif3 (for phytochrome-interacting factors) double mutant, which exhibits excessive singlet oxygen (1O₂) accumulation, and identified four different point mutants of the GENOMES UNCOUPLED 3 (GUN3, also known as HY2) gene encoding phytochromobilin synthase. Further genetic analysis revealed that either mutation of GUN3 or also GUN2 (encoding heme oxygenase, also known as HY1/HO1), but not FC1 (encoding ferrochelatase), rescues the cell death phenotype of pif1 pif3, lowers 1O₂ levels, and suppresses 1O₂-responsive gene expression. Furthermore, gun2 and gun3 mutations lead to heme-mediated feedback inhibition of 5-aminolevulinic acid synthesis and consequently reduced protochlorophyllide accumulation in the pif1 pif3 background. Notably, GUN2 and GUN3 physically interact with GUN4, while their mutations markedly correspond with decreased content of GUN4 and GUN5, which are involved in Mg chelation at the beginning of chlorophyll biosynthesis. Our studies point to an important regulatory role of GUN2 and GUN3 for the mutual link of heme and chlorophyll synthesis.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148520751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vascular bundle connections between pedicel and inflorescence stem in Arabidopsis revealed by a block-face serial fluorescence microscopy at a cellular resolution. 在细胞分辨率下用块面连续荧光显微镜观察拟南芥花梗和花序茎之间的维管束连接。

IF 4.7 2区生物学

Plant and Cell Physiology Pub Date : 2026-07-17 DOI: 10.1093/pcp/pcag098

Dongbo Shi, Florian Trier, Matias Volman, Atsushi Kasai

引用次数: 0

Live-Cell Imaging Enables Reporter-Free Monitoring Of The Circadian Rhythm In Individual Synechocystis Cells. 活细胞成像使报告自由监测昼夜节律在单个胞囊细胞。

IF 4.7 2区生物学

Plant and Cell Physiology Pub Date : 2026-07-17 DOI: 10.1093/pcp/pcag099

Lennart Witting, Florian P Stirba, Julius Nohr, Ekaterina Ivanova, Petra Kolkhof, Dietrich Kohlheyer, Anika Wiegard, Ilka M Axmann

{"title":"Live-Cell Imaging Enables Reporter-Free Monitoring Of The Circadian Rhythm In Individual Synechocystis Cells.","authors":"Lennart Witting, Florian P Stirba, Julius Nohr, Ekaterina Ivanova, Petra Kolkhof, Dietrich Kohlheyer, Anika Wiegard, Ilka M Axmann","doi":"10.1093/pcp/pcag099","DOIUrl":"https://doi.org/10.1093/pcp/pcag099","url":null,"abstract":"In vivo monitoring of circadian rhythms depends on reliable and non-invasive detection methods. This is often achieved by expressing reporter genes heterologously under the control of a circadian promoter. The activity or fluorescence of the gene product is then used as a readout. To avoid the generation of such reporter strains, we recently established a reporter-free detection method for cyanobacterial batch cultures. To determine whether these rhythms are driven at the level of individual cells or result from population-based effects, such as gating of cell division, we analysed individual Synechocystis sp. PCC 6803 cells by combining a microfluidic cultivation technique with time-lapse microscopy imaging. Hundreds of time-lapse image sequences were processed using our deep learning cell segmentation workflow. Although the cells had been entrained by a 12-hour light-dark cycle, cell division did not display a circadian rhythm. This indicates the absence of circadian gating of cell division in Synechocystis. Instead, we observed a circadian oscillation in the average brightness of the phase contrast in individual Synechocystis cells. To demonstrate how phase-contrast analysis of single cells can be complemented by backscatter analysis of batch cultures, we investigated the wildtype, a deletion mutant known to affect circadian rhythms (∆kaiC3) and complementation strains. We concluded that phase contrast and backscatter likely measured the same rhythmic changes in the refractive index of the cells. The method presented here will advance circadian research by enabling the analysis of circadian rhythms in individual cells without the need for expression of reporter molecules.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148472517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and Transcription Analysis of Phenolic Acid Biosynthesis Pathway in Pseudotaxus chienii. 伪红豆杉酚酸生物合成途径的鉴定与转录分析。

IF 4.7 2区生物学

Plant and Cell Physiology Pub Date : 2026-07-16 DOI: 10.1093/pcp/pcag097

Xiaori Zhan, Zijing Zhou, Yuting Peng, Xinru Wei, Zixin Shao, Yanheng Hu, Yanjun Yang, Chenjia Shen

{"title":"Identification and Transcription Analysis of Phenolic Acid Biosynthesis Pathway in Pseudotaxus chienii.","authors":"Xiaori Zhan, Zijing Zhou, Yuting Peng, Xinru Wei, Zixin Shao, Yanheng Hu, Yanjun Yang, Chenjia Shen","doi":"10.1093/pcp/pcag097","DOIUrl":"https://doi.org/10.1093/pcp/pcag097","url":null,"abstract":"Pseudotaxus chienii, an endangered relic conifer endemic to China, possesses significant medicinal potential, yet its phenolic acid biosynthesis and glycosylation mechanisms remain unelucidated. This study integrated metabolomics, mass spectrometry imaging (MSI), transcriptomics, and molecular biology to systematically characterize the phenolic acid pathway in P. chienii. Untargeted metabolomics identified distinct phenolic acid accumulation patterns between P. chienii and Taxus mairei. MALDI-2 MSI visualized ten phenolic acids, confirming leaves as the primary accumulation site. Genome-wide analysis revealed a complete phenolic acid biosynthesis pathway with key enzyme-encoding genes (4 PAL, 5 CCR, 2 F5H, etc.), while HQT was absent, indicating loss of chlorogenic acid synthesis. We identified 504 glycosyltransferase (GT) genes, with 48 leaf-specific ones (predominantly GT1 subfamily). Promoter and correlation analyses highlighted WRKY (PichiChr12G332970.1) and ERF (PichiChr3G086100.1) as core transcription factors (TFs). EMSA and dual-luciferase assays validated WRKY(PichiChr12G332970.1) directly activating three GT genes. This study clarifies the molecular basis and transcriptional regulation of phenolic acid glycosylation in P. chienii, providing a framework for exploiting its medicinal resources and guiding conservation-oriented breeding.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148472474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Co-option of developmental receptor-ligand signalling in plant symbiosis. 植物共生中发育受体-配体信号的共选择。

IF 4.7 2区生物学

Plant and Cell Physiology Pub Date : 2026-07-16 DOI: 10.1093/pcp/pcag094

Álvaro Daniel Fernández-Fernández

引用次数: 0

The extracellular regulatory network and key factors governing early embryogenesis in Nicotiana tabacum. 烟草早期胚胎发生的细胞外调控网络及关键因素。

IF 4.7 2区生物学

Plant and Cell Physiology Pub Date : 2026-07-08 DOI: 10.1093/pcp/pcag095

An Luo, Ying Qiao, Siyuan Li, Xinyue Wang, Ce Shi, Pan Luo

{"title":"The extracellular regulatory network and key factors governing early embryogenesis in Nicotiana tabacum.","authors":"An Luo, Ying Qiao, Siyuan Li, Xinyue Wang, Ce Shi, Pan Luo","doi":"10.1093/pcp/pcag095","DOIUrl":"https://doi.org/10.1093/pcp/pcag095","url":null,"abstract":"The plant extracellular space, including the cell wall, is a dynamic signaling compartment outside the plasma membrane that plays crucial roles in cell-cell communication and developmental coordination. Although early embryogenesis is known to be orchestrated by both intracellular and extracellular cues, the molecular mechanisms underlying extracellular regulation remain largely unexplored. Here, we report that NtProRP1, an extracellular protein, localizes to the cell wall immediately after fertilization in Nicotiana tabacum. Targeted knockout of NtProRP1 using the CRISPR-Cas9 system resulted in arrested embryos with irregular cell shapes and aberrant division patterns in aborted seeds. Furthermore, we performed high-resolution transcriptome profiling on a limited number of isolated two-celled proembryos from wild-type (WT) and Ntprorp1 plants, enabling the detection of early transcriptional changes with minimal interference from surrounding tissues. Genes involved in the synthesis and modification of cell wall components, including cellulose, hemicellulose, and pectin, were coordinately upregulated at the onset of embryogenesis in the Ntprorp1 mutant. Further analysis suggests a possible role for NtProRP1 in cell wall formation via the cell wall integrity (CWI) maintenance mechanism, potentially through the involvement of components such as receptor-like kinases (RLKs), mitogen-activated protein kinases (MAPKs), Ca2+ flux, and multiple phytohormones. Together, these results uncover a new mechanism for NtProRP1 in regulating early embryogenesis and deliver a unique transcriptomic resource that advances understanding of extracellular signaling in plant embryogenesis.","PeriodicalId":20575,"journal":{"name":"Plant and Cell Physiology","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148405513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Flexible, Top-Down Approach To Assays Of Leaf Movement Rhythms. 一个灵活的，自上而下的方法来分析叶片运动节奏。

IF 4.7 2区生物学

Plant and Cell Physiology Pub Date : 2026-07-04 DOI: 10.1093/pcp/pcag091

Ann Feke, Eva M Farré

引用次数: 0