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[Synthesis of exo-β-glucosaminidase BY FUNGUS Penicillium sp. IB-37-2]. [真菌Penicillium sp. IB-37-2合成外-β-氨基葡萄糖酶]。
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-09-01
G E Aktuganov, N F Galimzyanova, G A Teregulova, A I Melentjev
{"title":"[Synthesis of exo-β-glucosaminidase BY FUNGUS Penicillium sp. IB-37-2].","authors":"G E Aktuganov,&nbsp;N F Galimzyanova,&nbsp;G A Teregulova,&nbsp;A I Melentjev","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A new strain Penicillium sp. IB-37-2, which actively hydrolyzes chitosan (SD ∼80–85%) but possesses low activity against colloidal chitin, was isolated. The fungus was observed to have a high level chitosanase biosynthesis (1.5–3.0 U/mL) during submerged cultivation at 28°C, with a pH of 3.5–7.0 and 220 rpm in nutrient media containing chitosan or chitin from shells of crabs. Purification of the chitosanase enzyme complex from Penicillium sp. IB-37-2 by ultrafiltration and hydrophobic chromatography, followed by denaturing electrophoresis, revealed two predominant proteins with molecular weights of 89 and 41 kDa. The purified enzyme complex demonstrated maximal activity (maximal rate of hydrolysis of dissolved chitosan) and stability at 50–55°C and a pH of 3.5–4.0. The enzyme preparation also hydrolyzed laminarin, β-(1,3)-(1,4)-glycan, and colloidal chitin. Exohydrolysis of chitosan by the preparation isolated from Penicillium sp. IB-37-2 resulted in the formation of single product, D-glucosamine.</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35890348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Inhibition of IAA oxidase activity of wheat anionic peroxidase by chitooligosaccharides]. [壳寡糖对小麦阴离子过氧化物酶IAA氧化酶活性的抑制作用]。
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-09-01
I V Maksimov, Z R Usupova, E A Cherepanova, R M Khairulin, V A Vakhitov
{"title":"[Inhibition of IAA oxidase activity of wheat anionic peroxidase by chitooligosaccharides].","authors":"I V Maksimov,&nbsp;Z R Usupova,&nbsp;E A Cherepanova,&nbsp;R M Khairulin,&nbsp;V A Vakhitov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The study demonstrated that chitooligosaccharides with a molecular weight of 5–10 kDa and a degree of acetylation of 65% exhibited an auxin-like effect in wheat plants and also played an important role in regulating the activity of polysaccharide (chitin)–specific anion isoenzymes of peroxidase oxidizing indole acetic acid. Changes in the kinetic parameters of the interaction of the wheat anionic chitin-specific peroxidase with pI ∼3.5 with chitin oligomers in the presence of indoleacetic acid were pH-depended and indicated that chitooligosaccharides significantly impair the ability of the enzyme for oxidation at pH levels of 4.2 and 6.0. It can be assumed that chitooligosaccharides not only induce protective plant systems but also increase the accumulation of auxin in plant tissues, thus adversely affecting a number of components of the plant protective system against pathogens.</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35890712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Mechanisms of the interaction of chitosan and its derivatives with the cell]. 壳聚糖及其衍生物与细胞相互作用的机制。
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-09-01
A A Zubareva, E V Svirshchevskaya
{"title":"[Mechanisms of the interaction of chitosan and its derivatives with the cell].","authors":"A A Zubareva,&nbsp;E V Svirshchevskaya","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The review summarizes the data on the mechanisms of the interaction of chitosan and other molecules, as well as the drug delivery systems based on them, with mammalian cells. The mechanisms of binding, endocytosis, and the further distribution of chitosan and nanoparticles with different physic-chemical properties in cells remain unclear at the present time.</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35890954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Compatibility of cells of the nervous system with structured biodegradable chitosan-based hydrogel matrices]. [神经系统细胞与结构可生物降解的壳聚糖基水凝胶基质的相容性]。
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-09-01
P S Timashev, K N Bardakova, N V Minaev, T S Demina, T A Mishchenko, E V Mitroshina, A A Akovantseva, A A Koroleva, D S Asyutin, L F Pimenova, N A Konovalov, T A Akopova, A B Solov'eva, I V Mukhina, M V Vedunova, B N Chichkov, V N Bagratashvili
{"title":"[Compatibility of cells of the nervous system with structured biodegradable chitosan-based hydrogel matrices].","authors":"P S Timashev,&nbsp;K N Bardakova,&nbsp;N V Minaev,&nbsp;T S Demina,&nbsp;T A Mishchenko,&nbsp;E V Mitroshina,&nbsp;A A Akovantseva,&nbsp;A A Koroleva,&nbsp;D S Asyutin,&nbsp;L F Pimenova,&nbsp;N A Konovalov,&nbsp;T A Akopova,&nbsp;A B Solov'eva,&nbsp;I V Mukhina,&nbsp;M V Vedunova,&nbsp;B N Chichkov,&nbsp;V N Bagratashvili","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Hydrogel matrices for cell cultivation have been generated by two-photon laser polymerization of unsaturated chitosan derivatives and methacrylated hyaluronic acid. The adhesive and toxic properties of the matrices have been assessed, and the matrices have been shown to have a good compatibility with primary hippocampal cell cultures. The formation of morphologically normal neural networks by cells of the nervous system cultured on the surface of hydrogel matrices has been observed. The metabolic status of dissociated hippocampal cells cultured on the matrices was similar to that of the control cultures, as shown by the results of MTT reductase activity assay. Thus, matrices based on unsaturated polysaccharide derivatives crosslinked by laser irradiation showed good compatibility with differentiated cells of the nervous system and considerable potential for use in neurotransplantation.</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35890960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The effect of high-molecular weight chitosans on the antioxidant and immune systems of the honeybee]. [高分子量壳聚糖对蜜蜂抗氧化和免疫系统的影响]。
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-09-01
E S Saltykova, A A Karimova, A R Gataullin, L R Gaifullina, R T Matniyazov, M A Frolova, A I Albulov, A G Nikolenko
{"title":"[The effect of high-molecular weight chitosans on the antioxidant and immune systems of the honeybee].","authors":"E S Saltykova,&nbsp;A A Karimova,&nbsp;A R Gataullin,&nbsp;L R Gaifullina,&nbsp;R T Matniyazov,&nbsp;M A Frolova,&nbsp;A I Albulov,&nbsp;A G Nikolenko","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>High-molecular weight chitosan (200 kDa, 75% deacetylated) and N-succinoyl chitosan (300 kDa, 75% deacetylated) were shown to have a preadaptive effect and increase the lifespan of honeybees due to the induction of protective antioxidant and immune mechanisms. Chitosan with a molecular weight of 200 kDa had a fungistatic effect on a pathogenic fungus that causes ascospherosis, a disease of bee larvae and pupae.</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35890713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Antimutagenic systems based on chitosan conjugates with plant antioxidants]. [壳聚糖与植物抗氧化剂结合的抗诱变系统]。
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-09-01
V A Alexandrova, N S Domnina, G P Snigireva
{"title":"[Antimutagenic systems based on chitosan conjugates with plant antioxidants].","authors":"V A Alexandrova,&nbsp;N S Domnina,&nbsp;G P Snigireva","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>It was shown using various methods that the radical scavenging and radical scavenging activities of chitosan conjugates with phenolic antioxidants of plant origin significantly exceed the corresponding values of their low molecular weight analogs (gallic and syringic acids, quercetin and dihydroquercetin). Cytogenetic analysis of human peripheral blood lymphocytes demonstrated that the conjugates showed pronounced antimutagenic efficiency when the cells were γ-irradiated in vitro at a dose of 2 Gy.</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35890957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Analysis of toxicity and biocompatibility of chitosan derivatives with different physico-chemical properties]. 不同理化性质壳聚糖衍生物的毒性和生物相容性分析。
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-09-01
E V Svirshchevskaya, A A Zubareva, A A Boiko, O A Shustova, M V Grechikhina, B Ts Shagdarova, V P Varlamov
{"title":"[Analysis of toxicity and biocompatibility of chitosan derivatives with different physico-chemical properties].","authors":"E V Svirshchevskaya,&nbsp;A A Zubareva,&nbsp;A A Boiko,&nbsp;O A Shustova,&nbsp;M V Grechikhina,&nbsp;B Ts Shagdarova,&nbsp;V P Varlamov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A comparative study of the toxicity and hemocompatibility of chitosan and its derivatives with different acetylation degrees, molecular masses, charges, and hydrophobicity has been performed. It has been shown that only positively charged chitosan derivatives activate platelets and suppress cell proliferation, regardless of the acetylation degree, molecular mass, and hydrophobicity. Chitosan quaternization decreases toxicity at a low degree of substitution and abruptly increases it at a high one. Negatively charged chitosan derivatives were nontoxic and compatible with blood components. It was concluded that the toxicity of chitosan and its derivatives is defined by their charge and solubility at a neutral pH.</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35890956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Enzymatic hydrolysis of chitosan films in water and physiological solution]. [壳聚糖膜在水和生理溶液中的酶解]。
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-09-01
V V Chernova, I F Tuktarova, E I Kulish
{"title":"[Enzymatic hydrolysis of chitosan films in water and physiological solution].","authors":"V V Chernova,&nbsp;I F Tuktarova,&nbsp;E I Kulish","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>It was shown that the processes of enzymatic hydrolysis of chitosan in aqueous acetic acid and on the surface of chitosan films in a solution of hyaluronidase in acetic acid are described by uniform kinetic constants. Kinetic parameters of enzymatic hydrolysis of the chitosan film samples in water and in physiological solution (Ringer–Locke’s solution) were determined. It was found that the introduction of medicinal agents and low-molecular-weight electrolytes to a chitosan-based film material reduces the rate of enzymatic hydrolysis of the films, which may indicate a possible increase in their service life when used on the wound surface.</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35890346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The obtainment and characteristics of Kalanchoe pinnata L. plants expressing the artificial gene of the cecropin P1 antimicrobial peptide]. [表达抗菌肽cecropin P1人工基因的凤尾莲植株的获得及特性]。
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-07-01
N S Zakharchenko, E B Rukavtsova, T V Shevchuk, O V Furs, S V Pigoleva, A A Lebedeva, I A Chulina, L K Baidakova, Ya I Bur'yanov
{"title":"[The obtainment and characteristics of Kalanchoe pinnata L. plants expressing the artificial gene of the cecropin P1 antimicrobial peptide].","authors":"N S Zakharchenko,&nbsp;E B Rukavtsova,&nbsp;T V Shevchuk,&nbsp;O V Furs,&nbsp;S V Pigoleva,&nbsp;A A Lebedeva,&nbsp;I A Chulina,&nbsp;L K Baidakova,&nbsp;Ya I Bur'yanov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Kalanchoe pinnata L. plants bearing an artificial CP1 gene encoding the cecropin P1 antimicrobial peptide have been obtained. The presence of the CP1 gene in the plant genome has been confirmed by PCR. Cecropin P1 synthesis in transgenic plants has been shown by MALDI mass spectrometry and Western blotting. The obtained plants have been highly resistant to bacterial and fungal phytopathogens, and their extracts have demonstrated antimicrobial activity towards human and animal pathogens. It has been shown that transgenic plants bearing the CP1 gene can be colonized by the beneficial associative microorganisms Methylovorus mays.</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35890461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Biosynthesis of silver nanoparticles with the participation of extracellular Mn-dependent peroxidase from Azospirillum]. [氮螺旋藻细胞外锰依赖过氧化物酶参与银纳米颗粒的生物合成]。
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-07-01
M A Kupryashina, E P Vetchinkina, V E Nikitina
{"title":"[Biosynthesis of silver nanoparticles with the participation of extracellular Mn-dependent peroxidase from Azospirillum].","authors":"M A Kupryashina,&nbsp;E P Vetchinkina,&nbsp;V E Nikitina","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The accumulation of nanoparticles of colloidal silver with spherical shape in culture liquid of Azospirillum brasilense has been shown by transmission electron microscopy. Bacterial extracellular Mn-peroxidases were found to participate in silver reduction from silver nitrate with the formation of nanoparticles. A mechanism of extracellular biosynthesis of silver nanoparticles by A. brasilense bacteria was proposed</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35890564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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