Prikladnaia biokhimiia i mikrobiologiia最新文献_第3页

[Degradation of fluorene and fluoranthene by the basidiomycete Pleurotus ostreatus]. [担子菌平菇对芴和氟蒽的降解]。

Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-11-01

N N Pozdnyakova, M P Chernyshova, V S Grinev, E O Landesman, O V Koroleva, O V Turkovskaya

引用次数: 0

[Formation of protodioscin and deltoside isomers in suspension cultures of Nepal yam (Dioscorea deltoidea Wall.) cells]. [尼泊尔山药(Dioscorea deltoidea Wall.)细胞悬浮培养中原薯蓣皂苷和三角糖苷异构体的形成]。

Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-11-01

M T Khandy, M V Titova, S V Konstantinova, D V Kochkin, I M Ivanov, A M Nosov

{"title":"[Formation of protodioscin and deltoside isomers in suspension cultures of Nepal yam (Dioscorea deltoidea Wall.) cells].","authors":"M T Khandy, M V Titova, S V Konstantinova, D V Kochkin, I M Ivanov, A M Nosov","doi":"","DOIUrl":"","url":null,"abstract":"Changes in the content of the furostanol glycosides protodioscin and deltoside, particularly that of the (25S)-isomers of the glycosides, during suspension cultivation of different lines of Nepal yam (Dioscorea deltoidea Wall.) cells of the strain IFR-DM-0.5 has been investigated. The composition of furostanol glycosides has been characterized, and the dynamics of the accumulation of individual glycosides during lengthy subcultivation of cells maintained in flasks or in a barbotage bioreactor has been analyzed. A positive correlation between the growth and accumulation of substances that belonged to the class of furostanol glycosides has been demonstrated for cultured dioscorea cells, whereas the content of some of the individual glycosides varied considerably between the lines of the strain, cultures maintained under different conditions, and even between cells in different phases of the growth cycle. The increased content of (25R)-forms of the glycosides (protodioscin and deltoside) was correlated with a decrease in the cellular growth rate, whereas an increase in culture growth intensity occurred concomitantly to an increase of the amount of (25S)-isomers. This may be indicative of the specific stimulatory effect of (25S)-glycosides, but not the (25R)-forms, on cell proliferation in vitro. Thus, the concentration of (25S)-forms may increase due to the autoselection of cells capable of intensive division during prolonged cultivation.","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35892229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Cytokine-inducing and anti-inflammatory activity of chitosan and its low-molecular derivative]. 壳聚糖及其低分子衍生物的细胞因子诱导和抗炎活性。

Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-09-01

V N Davydova, A A Kalitnik, P A Markov, A V Volod'ko, S V Popov, I M Ermak

{"title":"[Cytokine-inducing and anti-inflammatory activity of chitosan and its low-molecular derivative].","authors":"V N Davydova, A A Kalitnik, P A Markov, A V Volod'ko, S V Popov, I M Ermak","doi":"","DOIUrl":"","url":null,"abstract":"A low-molecular derivative of the polysaccharide (5 kDa) was obtained and its cytokine-inducing and anti-inflammatory activity was studied by free radical depolymerization of chitosan (110 kDa). It was shown that high-molecular chitosan in vitro inhibited the synthesis of anti-inflammatory cytokine, the tumor necrosis factor alpha induced by endotoxin. In the case of peroral introduction to experimental animals, high- and low-molecular chitosans stimulated synthesis of the anti-inflammatory cytokine IL-10 in the blood serum of mice; in this case, the activity of the high-molecular derivative was two times higher as compared with the initial polysaccharide. With peroral introduction, the initial polysaccharide (50 mg/kg) and its derivative inhibited the development of chemically induced inflammation of experimental animals’ large intestines, which was manifested as a decrease in the affected area and the degree of damage to the large intestine wall, as well as a two-fold reduction of myeloperoxidase activity. According to morphological and biochemical characteristics, the effect of chitosans was similar to that of a hormone anti-inflammatory drug, prednisolone.","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35890958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Signal molecules involved in the regulation of the wheat defense response to Septoria nodorum infection]. [信号分子参与调节小麦对芽孢杆菌感染的防御反应]。

Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-09-01

L G Yarullina, R I Kasimova, I V Maksimov

引用次数: 0

[Biodegradablescaffolds based on chitosan: Preparation, properties, and use for the cultivation of animal cells]. [基于壳聚糖的可生物降解支架:制备、性能及其在动物细胞培养中的应用]。

Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-09-01

N R Kil’deeva, M A Kasatkina, M G Drozdova, T S Demina, S A Uspenskii, S N Mikhailov, E A Markvicheva

引用次数: 0

[Determination of antibacterial activity of chitosan]. 壳聚糖抑菌活性的测定。

Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-09-01

S N Kulikov, L T Bayazitova, O F Tyupkina, P V Zelenikhin, M M Salnikova, E A Bezrodnykh, V E Tikhonov