Prikladnaia biokhimiia i mikrobiologiia最新文献_第10页

[A New Approach to the Depletion of Albumin and Immunoglobulin G from Human Serum]. [从人血清中清除白蛋白和免疫球蛋白G的新方法]。

Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2015-05-01 DOI: 10.7868/s0555109915030046

E A Bormotova, B L Mil'man, T V Gupalova

引用次数: 0

[Determination of Azospirillum Brasilense Cells With Bacteriophages via Electrooptical Analysis of Microbial Suspensions]. [微生物悬浮液电光分析噬菌体测定巴西氮螺旋菌细胞]。

Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2015-05-01 DOI: 10.7868/s0555109915030083

O I Gulii, O A Karavayeva, S A Pavlii, O I Sokolov, V D Bunin, O V Ignatov

{"title":"[Determination of Azospirillum Brasilense Cells With Bacteriophages via Electrooptical Analysis of Microbial Suspensions].","authors":"O I Gulii, O A Karavayeva, S A Pavlii, O I Sokolov, V D Bunin, O V Ignatov","doi":"10.7868/s0555109915030083","DOIUrl":"https://doi.org/10.7868/s0555109915030083","url":null,"abstract":"The dependence-of changes in the electrooptical properties of Azospirillum brasilense cell suspension Sp7 during interaction with bacteriophage ΦAb-Sp7 on the number and time of interactions was studied. Incubation of cells with bacteriophage significantly changed the electrooptical signal within one minute. The selective effect of bacteriophage ΦAb on 18 strains of bacteria of the genus Azospirillum was studied: A. amazonense Ami4, A. brasilense Sp7, Cd, Sp107, Sp245, Jm6B2, Brl4, KR77, S17, S27, SR55, SR75, A. halopraeferans Au4, A. irakense KBC1, K A3, A. lipoferum Sp59b, SR65 and RG20a. We determined the limit of reliable determination of microbial cells infected with bacteriophage: - 10(4) cells/mL. The presence of foreign cell cultures of E. coli B-878 and E. coli XL-1 did not complicate the detection of A brasilense Sp7 cells with the use of bacteriophage ΦAb-Sp7. The results demonstrated that bacteriophage (ΦAb-Sp7 can be used for the detection of Azospirillum microbial cells via t electrooptical analysis of cell suspensions.","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34032678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Antioxidant Effect on the Growth And Formation of Lipids in the Fungus Lentinus tigrinus Growing on Medium with Lignosulfonate]. [木质素磺酸培养基对香菇生长及脂质形成的抗氧化作用]。

Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2015-05-01 DOI: 10.7868/s0555109915030101

A A Ivashechkin, J E Sergeeva, V V Lunin, J S Mysyakina, E P Feofilova

引用次数: 0

[Endophytic Bacteria in Microbial Drugs that Improve Plant Development (Review)]. [促进植物发育的微生物药物中的内生细菌(综述)]。

Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2015-05-01 DOI: 10.7868/s0555109915030058

V K Chebotar, N V Malfanova, A V Shcherbakov, G A Ahtemova, A Y Borisov, B Lugtenberg, I A Tihonovich

引用次数: 8

[Investigation of Protein Translocation Sec-System with Heterologous Gene Expression in Shewanella oneidensis MR-1 Bacterium Cells]. 单根希瓦氏菌MR-1细菌细胞中异源基因表达蛋白易位系统的研究

Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2015-05-01 DOI: 10.7868/s0555109915030125

N N Mordkovich, N A Okorokova, V P Veiko

引用次数: 0

[Cultivation of a novel cellulase/xylanase producer, Trichoderma longibrachiatum mutant TW 1-59-27: production of the enzyme preparation and the study of its properties]. [一种新型纤维素酶/木聚糖酶产生菌长achiatum木霉突变体TW 1-59-27的培养:酶制剂的生产及其特性研究]。

Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2015-03-01 DOI: 10.7868/s0555109915020038

A O Bekkarevicha, V A Nemashkalov, A V Koshelev, D A Goryazchev, T V Bubnova, V Yu Matys, D O Osipov, E G Kondrat'eva, O N Okunev, A P Sinitsyn

{"title":"[Cultivation of a novel cellulase/xylanase producer, Trichoderma longibrachiatum mutant TW 1-59-27: production of the enzyme preparation and the study of its properties].","authors":"A O Bekkarevicha, V A Nemashkalov, A V Koshelev, D A Goryazchev, T V Bubnova, V Yu Matys, D O Osipov, E G Kondrat'eva, O N Okunev, A P Sinitsyn","doi":"10.7868/s0555109915020038","DOIUrl":"https://doi.org/10.7868/s0555109915020038","url":null,"abstract":"As a result of gamma-mutagenesis of Trichoderma longibrachiatum TW1 and the subsequent selection of improved producers, a novel mutant strain, TW1-59-27, capable of efficiently secreting cellulase and xylanase was obtained. In a fed-batch cultivation, the new TW1-59-27 mutant was significantly more active compared with the original TW1 strain. For instance, the activities of cellulase (towards carboxymethylcellulose) and xylanase in the culture broth (CB) increased by 1.8 and two times, respectively, and the protein content increased by 1.47 times. The activity of these enzymes in the dry enzyme preparation derived from the CB of the TW1-59-27 mutant was 1.3-1.8 times higher than that in the preparation derived from the original TW1 strain. It was established that the cellulase from the enzyme preparation of the mutant strain demonstrated the maximum activity at 55-65 degrees C; it occurred in xylanase at 60 degrees C. The pH optima of these enzymes were pH 4.5-5.0 and pH 5.0-6.0, respectively. It was shown that the content of endoglucanases in the enzyme preparation increased from 7% to 13.5%; the effect is largely driven by the secretion of endoglucanase-1. An enzyme preparation with increased endoglucanase-1 content is promising for use as a feed additive in agriculture.","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33346983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[11beta-Hydroxylation of 6alpha-Fluoro-16alpha-Methyl-Deoxycorticosterone 21-Acetate by filamentous fungi]. [11 -丝状真菌对6 -氟-16 -甲基-脱氧皮质酮21-醋酸酯的羟基化]。

Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2015-03-01 DOI: 10.7868/s0555109915020105

V V Kollerov, V V Fokina, G V Sukhodolskaya, A A Shutov, M V Donova

{"title":"[11beta-Hydroxylation of 6alpha-Fluoro-16alpha-Methyl-Deoxycorticosterone 21-Acetate by filamentous fungi].","authors":"V V Kollerov, V V Fokina, G V Sukhodolskaya, A A Shutov, M V Donova","doi":"10.7868/s0555109915020105","DOIUrl":"https://doi.org/10.7868/s0555109915020105","url":null,"abstract":"Selected filamentous fungi--98 strains of 31 genera--were screened for the ability to catalyze 11beta-hydroxylation of 6alpha-fluoro-16alpha-methyl-deoxycorticosterone 21-acetate (FM-DCA). It was established that representatives of the genera Gongronella, Scopulariopsis, Epicoccum, and Curvularia have the ability to activate 11beta-hydroxylase steroids. The strains of Curvularia lunata VKM F-644 and Gongronella butleri VKM F-1033 expressed maximal activity and formed 6lpha-fluoro-16alpha-methyl-corticosterone as a major bioconversion product from FM-DCA. The structures of the major products and intermediates of the bioconversion were confirmed by TLC, H PLC, MS and 1H NMR analyses. Different pathways of 6alpha-fluoro-16alpha-methyl-corticosterone formation by C. lunata and G. butleri strains were proposed based on intermediate identification. The constitutive character and membrane-binding localization were evidence of a 11beta-hydroxylating system in G. butleri, while an inducible character and microsomal localization was confirmed for 11beta-hydroxylase of C. lunata. Under optimized conditions, the molar yield of 6alpha-fluoro-16alpha-methyl-corticosterone reached 65% at a FM-DCA substrate loading of 6 g/L.","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33347555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Biosynthesis of isocitric acid by the yeast yarrowia lipolytica and its regulation]. [解脂耶氏酵母合成异柠檬酸及其调控]。

Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2015-03-01 DOI: 10.7868/s0555109915020075

S V Kamzolova, Y N Lunin, R K Allayarov, I F Puntus, I A Laptev, V A Samojlenko, I G Morgunov

{"title":"[Biosynthesis of isocitric acid by the yeast yarrowia lipolytica and its regulation].","authors":"S V Kamzolova, Y N Lunin, R K Allayarov, I F Puntus, I A Laptev, V A Samojlenko, I G Morgunov","doi":"10.7868/s0555109915020075","DOIUrl":"https://doi.org/10.7868/s0555109915020075","url":null,"abstract":"We studied the biosynthesis of isocitric acid from rapeseed (canola) oil by the yeast Yarrowia lipolytica and its regulation. We determined a fundamental possibility for directed biosynthesis of isocitric acid by Y lipolytica yeast, with only minimal amounts of citric acid byproduct, when grown on a medium containing canola oil. Wild type strains of Y lipolytica were mutagenized by UV irradiation and treatment with N-methyl-N'-nitro-N-nitrosoguanidine (NG). Subsequent selection on media with acetate and isocitrate resulted in isolation of a UV/NG Y lipolytica UV/NG mutant that synthesized isocitrate and citrate at a ratio of 2.7:1. In the parent strain, this ratio is 1:1. Inhibition of isocitrate lyase, a key enzyme in the metabolism of isocitric acid, by the addition of itaconic acid resulted in increased synthesis of isocitrate with a ratio of isocitrate to citrate reaching 6:1. Culturing of the Y lipolytica UV/NG mutant in a pilot industrial fermenter in the presence of itaconic acid resulted in the production of 88.7 g/L of isocitric acid with a yield of 90%.","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33348958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Oil biodegradation by microbial-plant associations]. [微生物与植物结合的石油生物降解]。

Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2015-03-01 DOI: 10.7868/s0555109915020063

A A Ivanova, A A Vetrova, A E Filonov, A M Boronin

引用次数: 0

[Physiological and biochemical properties of actinobacteria as the basis of their high biodegradative activity (review)]. 放线菌的生理生化特性是其高生物降解活性的基础(综述)。

Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2015-03-01 DOI: 10.7868/s0555109915020208

I P Solvanikova, L A Golovleva

引用次数: 0