Prikladnaia biokhimiia i mikrobiologiia最新文献_第9页

[Inhibition of Linseed Oil Autooxidation by Essential Oils and Extracts from Spice Plants]. [香料植物的精油和提取物对亚麻籽油自氧化的抑制作用]。

Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2015-07-01

T A Misharina, E S Alinkina, M B Terenina, N I Krikunova, V I Kiseleva, I B Medvedeva, M G Semenova

引用次数: 0

A NOVEL OLEAGINOUS YEAST STRAIN WITH HIGH LIPID PRODUCTIVITY AND ITS APPLICATION TO ALTERNATIVE BIODIESEL PRODUCTION. 一株高脂质产油酵母菌及其在替代生物柴油生产中的应用。

Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2015-07-01 DOI: 10.7868/s0555109915030034

A Areesirisuk, C H Chiu, T B Yen, C H Liu, J H Guo

引用次数: 0

[Effect of pH of Adsorption Buffers on the Number and Antigen-Binding Activity of Monoclonal Antibodies Immobilized on the Surface of Polystyrene Microplates]. [吸附缓冲液pH对固定在聚苯乙烯微孔板表面的单克隆抗体数量和抗原结合活性的影响]。

Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2015-07-01

Yu N Tarakanova, A D Dmitriev, Yu S Massino, A A Pechelulko, O L Segal, Yu O Skoblov, T I Ulanova, V F Lavrov, D A Dmitriev

{"title":"[Effect of pH of Adsorption Buffers on the Number and Antigen-Binding Activity of Monoclonal Antibodies Immobilized on the Surface of Polystyrene Microplates].","authors":"Yu N Tarakanova, A D Dmitriev, Yu S Massino, A A Pechelulko, O L Segal, Yu O Skoblov, T I Ulanova, V F Lavrov, D A Dmitriev","doi":"","DOIUrl":"","url":null,"abstract":"The change in the concentration and antigen-binding activity of 28 monoclonal antibodies was studied after their adsorption on the surface of polystyrene microplates in buffers with different pH values (1.0, 2.8, 7.5, 9.6, and 11.9). We used 16 clones to the HIV p24 protein and 12 clones to the surface antigen of Hepatitis B Virus. The binding efficiency of adsorbed antibodies to the labeled antigen was evaluated by the slope of the linear region of the binding curve to the concentration axis. It was shown that the antigen-binding activity of six antibodies (21.5%) statistically significantly increased after adsorption at pH 2.8 and 11.9 as compared to pH 7.5 and 9.5. The maximum amount of antibodies was found to be adsorbed on the solid surface at pH 7.5. The analysis of the binding of 125I-HBs-antigen to adsorbed antibodies made it possible to evaluate the concentration of active antibodies on the polystyrene surface. It was shown that the increase in the antigen-binding activity was due to an increase in the proportion of antibodies with retained activity after adsorption at pH 2.8 and 11.9. Under these conditions, about 20% of the antibodies retained their antigen-binding activity, and 6% did so after immobilization at pH 7.5.","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34160381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Intensification of Microbial Decomposition of Organic Fraction of Municipal Waste: Laboratory and Field Experiments]. [强化微生物对城市垃圾有机组分的分解:实验室和现场试验]。

Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2015-07-01

A A Nikitina, M V Kevbrina, A Yu Kallistova, V K Nekrasova, Yu V Litti, A N Nozhevnikova

{"title":"[Intensification of Microbial Decomposition of Organic Fraction of Municipal Waste: Laboratory and Field Experiments].","authors":"A A Nikitina, M V Kevbrina, A Yu Kallistova, V K Nekrasova, Yu V Litti, A N Nozhevnikova","doi":"","DOIUrl":"","url":null,"abstract":"Methods of intensifying the anaerobic microbial decomposition of the organic fraction of municipal solid waste (MSW) on an MSW landfill and in anaerobic reactors were studied. It was discovered that it is preferable for the initiation and stabilization of the process of anaerobic digestion of organic waste in laboratory bioreactors at 20 and 50 degrees C to use a mixture of activated suspension of soil from the anaerobic zone of the landfill and digested sewage sludge. Stimulation of methanogenesis was shown in field conditions when digested sewage sludge was added directly into the upper layer of anaerobic zone of the landfill. The investigation of methane production during fermentation of concentrated food waste with a mixture of excessive activated sludge in the laboratory under thermophilic conditions (50 degrees C) has shown that the main problem at the first stage of the process was the acidification of the digested mixture due to the accumulation of volatile fatty acids. It was shown that for stable operation of the bioreactor under thermophilic conditions the amount of inoculum added during the start up should be no less than 30%-50%--based on volatile suspended solids. A sharp decrease in the digestion temperature from 50 to 20 degrees C did not cause methanogenesis termination, since the thermophilically fermented biomass contained both thermophilic and mesophilic methanogens.","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34158853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Biologically Active Peptides of King Crab Hepatopancreas]. 帝王蟹肝胰脏的生物活性肽。

Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2015-07-01

V V Bogdanov, B B Berezin, A P Il'ina, V P Yamskova, I A Yamskov

引用次数: 0

[Biologically Active Peptides Isolated from Dill Anethum graveolens L]. [d] Anethum graveolens的生物活性肽[j]。

Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2015-05-01 DOI: 10.7868/s0555109915030113

O G Kulikova, D I Maltsev, A P Ilyina, A V Burdina, V P Yamskova, I A Yamskov

引用次数: 0

[Biotechnological Applications of the Extremophilic Yeast Yarrowia lipolytica (Review)]. [嗜极酵母解脂耶氏菌的生物技术应用[j]。

Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2015-05-01 DOI: 10.7868/s0555109915030150

V Yu Sekova, E P Isakova, Yu I Deryabina

引用次数: 0

[The Engineering of a Yarrowia lipolytica Yeast Strain Capable of Homologous Recombination of the Mitochondrial Genome]. [能同源重组线粒体基因组的多脂耶氏菌酵母菌的工程研究]。

Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2015-05-01 DOI: 10.7868/s0555109915030095

E P Isakova, E Yu Epova, V Yu Sekova, E V Trubnikova, Yu K Kudykina, M V Zylkova, M A Guseva, Yu I Deryabina

{"title":"[The Engineering of a Yarrowia lipolytica Yeast Strain Capable of Homologous Recombination of the Mitochondrial Genome].","authors":"E P Isakova, E Yu Epova, V Yu Sekova, E V Trubnikova, Yu K Kudykina, M V Zylkova, M A Guseva, Yu I Deryabina","doi":"10.7868/s0555109915030095","DOIUrl":"https://doi.org/10.7868/s0555109915030095","url":null,"abstract":"None of the studied eukaryotic species has a natural system for homologous recombination of the mitochondrial genome. We propose an integrated genetic construct pQ-SRUS, which allows introduction of the recA gene from Bacillus subtilis into the nuclear genome of an extremophilic yeast, Yarrowia lipolytica. The targeting of recombinant RecA to the yeast mitochondria is provided by leader sequences (5'-UTR and 3'-UTR) derived from the SOD2 gene mRNA, which exhibits affinity to the outer mitochondrial membrane and thus provides cotranslational transport of RecA to the inner space of the mitochondria. The Y. lipolytica strain bearing the pQ-SRUS construct has the unique ability to integrate DNA constructs into the mitochondrial genome. This fact was confirmed using a tester construct, pQ-NIHN, intended for the introduction of the EYFP gene into the translation initiation region of the Y. lipolytica ND1 mitochondrial gene. The Y. lipolytica strain bearing pQ-SRUS makes it possible to engineer recombinant producers based on Y. lipolytica bearing transgenes in the mitochondrial genome. They are promising for the construction of a genetic system for in vivo replication and modification of the human mitochondrial genome. These strains may be used as a tool for the treatment of human mitochondrial diseases (including genetically inherited ones).","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34032679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Antimicrobial Activity of Fungi Strains of Trichoderma from Middle Siberia]. [中西伯利亚木霉菌种的抑菌活性]。

Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2015-05-01 DOI: 10.7868/s0555109915030149

V S Sadykova, A V Kurakov, A E Kuvarina, E A Rogozhin

引用次数: 0

[Quantitative Assessment of the Combined Effect of the Nitrogen Status, Light and Dehydration of Mycelium on Conidiation in Neurospora crassa]. [氮态、光照和菌丝体脱水对粗神经孢子菌孢子作用的定量评价]。

Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2015-05-01 DOI: 10.7868/s055510991503006x

S Filippovich, G Bachurina, D Shcherbakov

{"title":"[Quantitative Assessment of the Combined Effect of the Nitrogen Status, Light and Dehydration of Mycelium on Conidiation in Neurospora crassa].","authors":"S Filippovich, G Bachurina, D Shcherbakov","doi":"10.7868/s055510991503006x","DOIUrl":"https://doi.org/10.7868/s055510991503006x","url":null,"abstract":"We performed a quantitative assessment of the conidia yield in Neurospora crassa in response to treatment with different conidiation effectors. Depending on nitrogen source and intactness of nitrite reductase (NiR) and nitrate reductase (NR), light and dehydration affected the number of viable conidia produced by the ascomycete. In most variants of the nitrogen status, the combined action of light and dehydration synergistically increased the conidia yield. Conidiation in wild-type cells cultivated on the medium with NH4Cl as a sole nitrogen source did not respond to light, whereas illumination of the same culture grown on NH4NO3- or NaNO3-containing medium stimulated the process of spore formation. In response to light exposure, conidia formation occurred in the same way in the nit-2 (no NR and NiR) and nit-6 (no NiR) mutants cultivated in the presence of NH4Cl, but differed greatly when grown on the medium with NH4NO3. The results obtained indicate the possibility that NR and NiR participate in the photoconidiation regulation (wild-type strain on the medium with secondary nitrogen source); however, they cannot be necessary because light-dependent stimulation of spore formation was observed in nit-2 and nit-6 mutants.","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34034206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0