Prikladnaia biokhimiia i mikrobiologiia最新文献

[Peroxidase activity of octaheme nitrite reductases from bacteria of the Thioalkalivibrio genus]. [硫代碱弧菌属细菌亚硝酸盐八血红素还原酶的过氧化物酶活性]。

Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2017-03-01

T V Tikhonova, E S Slutskaya, V O Popov

{"title":"[Peroxidase activity of octaheme nitrite reductases from bacteria of the Thioalkalivibrio genus].","authors":"T V Tikhonova, E S Slutskaya, V O Popov","doi":"","DOIUrl":"","url":null,"abstract":"Closely related penta- and octaheme nitrite reductases catalyze the reduction of nitrite, nitric oxide, and hydroxylamine to ammonium and of sulfite to sulfide. NrfA pentaheme nitrite reductase plays the key role in anaerobic nitrate respiration and the protection of bacterial cells from stresses caused by nitrogen oxides and hydrogen peroxide. Octaheme nitrite reductases from bacteria of the Thioalkalivibrio genus are less studied, and their function in the cell is unknown. In order to estimate the possible role of octaheme nitrite reductases in the cell resistance to oxidative stress, the peroxidase activity of the enzyme from T. nitratireducens (TvNiR) has been studied in detail. Comparative analysis of the active site structure of TvNiR and cytochrome c peroxidases has shown some common features, such as a five-coordinated catalytic heme and identical catalytic residues in active sites. A model of the possible productive binding of peroxide at the active site of TvNiR has been proposed. The peroxidase activity has been measured for TvNiR hexamers and trimers under different conditions (pH, buffers, the addition of CaCl2 and EDTA). The maximum peroxidase activity of TvNiR with ABTS as a substrate (k cat = 17 s–1; k cat/K m = 855 mM–1 s–1) has been 100–300 times lower than the activity of natural peroxidases. The different activities of TvNiR trimers and hexamers indicate that the rate-limiting stage of the reaction is not the catalytic event at the active site but the electron transfer along the heme c electron-transport chain.","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35887278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Flavonoid oxidation kinetics in aqueous and aqueous organic media in the presence of peroxidase, tyrosynase, and hemoglobin]. [在有过氧化物酶、酪氨酸酶和血红蛋白存在的水性和水性有机介质中的类黄酮氧化动力学]。

Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2017-03-01

M E Barsukova, A I Tokareva, T S Buslova, L I Malinina, I A Veselova, T N Shekhovtsova

引用次数: 0

[Functionality of Metdi5511gene in Methylobacterium dichloromethanicum DM4]. [metdi5511基因在二氯甲烷甲基杆菌DM4中的功能研究]。

Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2017-03-01

Yu E Firsova, M L Torgonskaya, Yu A Trotsenko

引用次数: 0

[Production of Methane in Anaerobic Digestion of Organic Waste of Vegetable Processing Facilities]. [蔬菜加工设备有机废弃物厌氧消化产生甲烷]。

Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2017-03-01

M A Gladchenko, D A Kovalev, Yu V Litti, A N Nozhevnikova

引用次数: 0

[The new bacterial strain Paenibacillus sp. IB-1: A producer of exopolysaccharide and biologically active substances with phytohormonal and antifungal activities]. [新菌株Paenibacillus sp. IB-1:具有植物激素和抗真菌活性的胞外多糖和生物活性物质的生产者]。

Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2017-03-01

M D Bakaeva, S P Chetverikov, T Yu Korshunova, O N Loginov

{"title":"[The new bacterial strain Paenibacillus sp. IB-1: A producer of exopolysaccharide and biologically active substances with phytohormonal and antifungal activities].","authors":"M D Bakaeva, S P Chetverikov, T Yu Korshunova, O N Loginov","doi":"","DOIUrl":"","url":null,"abstract":"The bacterial strain IB-1, which exhibits antagonism towards phytopathogens and stimulates the growth of agricultural plants, was isolated from the soil. Analysis of the cultural, morphological, physiological, and biochemical features and nucleotide sequences of the genes 16S rRNA and gyrB, as well as the fatty acid composition, made it possible to attribute the strain IB-1 to the genus Paenibacillus; however, the results did not provide an unambiguous conclusion on its species. The strain Paenibacillus sp. IB-1 possesses nitrogenase activity, the ability to synthesize indoleacetic acid and cytokinin-like compounds, and antagonistic activity towards phytopathogenic fungi, which indicates prospects for its use as a biological product for agricultural purposes. A high-viscous exopolysaccharide was isolated from the cultural fluid of Paenibacillus sp. IB-1. Based on the data from IR and NMR spectroscopy, it was shown to be a heteropolymer comprised of one to four linked α-L-guluronic acid and β-D-mannuronic acid residues. The exopolysaccharide was successfully tested as an adhesive for presowing treatment of barley and wheat seeds with biofungicides.","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35888006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Immunity of a leguminous plant infected by nodular bacteria Rhizobium spp. F.] 根瘤菌侵染豆科植物的免疫特性[j]。

Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2017-03-01

A K Glyan'ko, A A Ischenko

引用次数: 0

[Competition between redox mediator and oxygen in the microbial fuel cell]. [微生物燃料电池中氧化还原介质与氧的竞争]。

Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2017-03-01

S V Alferov, S V Vozchikova, V A Arlyapov, V A Alferov, A N Reshetilov

{"title":"[Competition between redox mediator and oxygen in the microbial fuel cell].","authors":"S V Alferov, S V Vozchikova, V A Arlyapov, V A Alferov, A N Reshetilov","doi":"","DOIUrl":"","url":null,"abstract":"The maximal rates and effective constants of 2,6-dichlorphenolindophenol and oxygen reduction by bacterim Gluconobacter oxydans in bacterial fuel cells under different conditions were evaluated. In an open-circuit mode, the rate of 2,6-dichlorphenolindophenol reduction coupled with ethanol oxidation under oxygen and nirogen atmospheres were 1.0 and 1.1 μM s–1 g–1, respectively. In closed-circuit mode, these values were 0.4 and 0.44 μM s–1 g–1, respectively. The initial rate of mediator reduction with the use of membrane fractions of bacteria in oxygen and nitrogen atmospheres in open-circuit mode were 6.3 and 6.9 μM s–1 g–1, whereas these values in closed-circuit mode comprised 2.2 and 2.4 μM s–1 g–1, respectively. The oxygen reduction rates in the presence and absence of 2,6-dichlorphenolindophenol were 0.31 and 0.32 μM s–1 g–1, respectively. The data obtained in this work demonstrated independent electron transfer from bacterial redox centers to the mediator and the absence of competition between the redox mediator and oxygen. The results can make it possible to reduce costs of microbial fuel cells based on activity of acetic acid bacteria G. oxydans.","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35887430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Effect of lipopolysaccharide O-side chains on the adhesiveness of Yersinia pseudotuberculosis to J774 macrophages as revealed by optical trap]. [光学陷阱揭示的脂多糖o侧链对假结核耶尔森菌对J774巨噬细胞粘附性的影响]。

Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2017-03-01

A A Byvalov, V L Kononenko, I V Konyshev

引用次数: 0

[Synthesis of L-lactate oxidaze in yeast Yarrowia lipolytica during submerged cultivation]. [脂肪耶氏酵母深层培养过程中l -乳酸氧化酶的合成]。

Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2017-03-01

E N Biryukova, A Yu Arinbasarova, A G Medentsev

引用次数: 0

[Content of salicylic and jasmonic acids in pea roots (Pisum sativum L.) at the initial stage of symbiotic or pathogenic interaction with bacteria of the family Rhizobiaceae]. [豌豆根（Pisum sativum L.）与根瘤菌科细菌共生或致病作用初期的水杨酸和茉莉酸含量]。

Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2017-03-01

E G Rudikovskaya, G P Akimova, A V Rudikovskii, N B Katysheva, L V Dudareva

引用次数: 0