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[Nodulation competitiveness of nodule bacteria: Genetic control and adaptive significance]. 结瘤细菌的结瘤竞争力:遗传控制和适应意义。
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2017-03-01
O P Onishchuk, N I Vorobyov, N A Provorov
{"title":"[Nodulation competitiveness of nodule bacteria: Genetic control and adaptive significance].","authors":"O P Onishchuk,&nbsp;N I Vorobyov,&nbsp;N A Provorov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The most recent data on the system of cmp (competitiveness) genes that determine the nodulation competitiveness of rhizobial strains, i.e., the ability to compete for nodule formation in leguminous plants, is analyzed. Three genetic approaches for the construction of economically valuable strains of rhizobia are proposed: the amplification of positive regulators of competitiveness, the inactivation of the negative regulators of this trait, and the introduction of efficient competitiveness factors into strains capable of active nitrogen fixation.</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35885804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[PEGylated recombinant L-asparaginase from Erwinia carotovora: Production, properties, and potential applications]. [来自胡萝卜欧文菌的聚乙二醇化重组l -天冬酰胺酶:生产、性质和潜在应用]。
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2017-03-01
N S Melik-Nubarov, I D Grozdova, G Yu Lomakina, M V Pokrovskaya, V S Pokrovski, S S Aleksandrova, O Yu Abakumova, O V Podobed, D V Grishin, N N Sokolov
{"title":"[PEGylated recombinant L-asparaginase from Erwinia carotovora: Production, properties, and potential applications].","authors":"N S Melik-Nubarov,&nbsp;I D Grozdova,&nbsp;G Yu Lomakina,&nbsp;M V Pokrovskaya,&nbsp;V S Pokrovski,&nbsp;S S Aleksandrova,&nbsp;O Yu Abakumova,&nbsp;O V Podobed,&nbsp;D V Grishin,&nbsp;N N Sokolov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>N-hydroxysuccinimide ester of monomethoxy polyethylene glycol hemisuccinate was synthesized. It acylated amino groups in a molecule of recombinant L-asparaginase from Erwinia carotovora. A method of L-asparaginase modification by the obtained activated polyethylene glycol derivative was developed. The best results were produced by modification of the enzyme with a 25-fold excess of reagent relative to the enzyme tetramer. The modified L-asparaginase was isolated from the reaction mixture by gel filtration on Sepharose CL-6B. The purified bioconjugate did not contain PEG unbound to the protein, demonstrated high catalytic activity, and exhibited antiproliferative action on cell cultures.</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35887283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Novel biocatalyst for productions of S-(-)-2-[6-benzyloxy -2,5,7,8-tetramethylchroman -2-yl] ethanol—precursor of natural α-tocols]. [生产S-(-)-2-[6-苄基-2,5,7,8-四甲基铬-2-基]乙醇-天然α-工具前体的新型生物催化剂]。
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2017-03-01
N I Petukhova, I I Kon'shina, A Yu Spivak, V N Odinokov, V V Zorin
{"title":"[Novel biocatalyst for productions of S-(-)-2-[6-benzyloxy -2,5,7,8-tetramethylchroman -2-yl] ethanol—precursor of natural α-tocols].","authors":"N I Petukhova,&nbsp;I I Kon'shina,&nbsp;A Yu Spivak,&nbsp;V N Odinokov,&nbsp;V V Zorin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A novel promising strain of actinobacteria Rhodococcus sp. 77-32 was identified. Its acetonetreated biomass the could be used as a biocatalyst for production of S-(-)-2-[6-benzyloxy-2,5,7,8-tetramethylchroman-2-yl] ethanol (S-BCE), a precursor of natural α-tocols. It was established that a reaction of enantioselective hydrolysis of racemic (±)-2-(2-acetoxyethyl)-6-benzyloxy-2,5,7,8-tetramethylchroman (BCEA) occurred in the phosphate buffer–acetone system, resulting in enrichment of the residual substrate by S-enantiomer (S-(+)-2-(2-acetoxyethyl)-6-benzyloxy-2,5,7,8-tetramethylchroman, S-BCEA). It was shown that the hydrolysis was accompanied by stereoinversion of the formed product, R-(+)-2-[6-benzyloxy-2,5,7,8-tetramethylchroman-2-yl] ethanol (R-BCE), into the S-BCE. The transformation conditions (acetone content, acidity, temperature, reaction duration) were optimized, providing simultaneous production of optically pure S-BCE and S-BCEA with an almost quantitative yield.</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35887284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Genetic system for maintaining the mitochondrial human genome in yeast Yarrowia lipolytica]. [酵母多脂耶氏菌维持线粒体人类基因组的遗传系统]。
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-11-01
E P Isakova, Yu I Deryabina, A V Velyakova, J K Biryukova, V V Teplova, A B Shevelev
{"title":"[Genetic system for maintaining the mitochondrial human genome in yeast Yarrowia lipolytica].","authors":"E P Isakova,&nbsp;Yu I Deryabina,&nbsp;A V Velyakova,&nbsp;J K Biryukova,&nbsp;V V Teplova,&nbsp;A B Shevelev","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>For the first time, the possibility of maintaining an intact human mitochondrial genome in a heterologous system in the mitochondria of yeast Yarrowia lipolytica is shown. A method for introducing directional changes into the structure of the mitochondrial human genome replicating in Y. lipolytica by an artificially induced ability of yeast mitochondria for homologous recombination is proposed. A method of introducing and using phenotypic selection markers for the presence or absence of defects in genes tRNA-Lys and tRNA-Leu of the mitochondrial genome is developed. The proposed system can be used to correct harmful mutations of the human mitochondrial genome associated with mitochondrial diseases and for preparative amplification of intact mitochondrial DNA with an adjusted sequence in yeast cells. The applicability of the new system for the correction of mutations in the genes of Lys- and Leu-specific tRNAs of the human mitochondrial genome associated with serious and widespread human mitochondrial diseases such as myoclonic epilepsy with lactic acidosis (MELAS) and myoclonic epilepsy with ragged-red fibers (MERRF) is shown.</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35892230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Lignin: Chemical structure, biodegradation, and practical application]. [木质素:化学结构、生物降解和实际应用]
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-11-01
E P Feofilova, I S Mysyakina
{"title":"[Lignin: Chemical structure, biodegradation, and practical application].","authors":"E P Feofilova,&nbsp;I S Mysyakina","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The review describes the natural biopolymer lignin, which is second in plant biomass abundance. It is evident now that lignin is considerably undervalued and insufficiently studied in the applied area. The review focuses on the history of the lignin discovery, methods for its extraction from plant objects, its biodegradation by fungi, the enzymes degrading lignin, and the prospects of its application in current biotechnology.</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35890719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Fluorescence polarization immunoassay of ractopamine]. 莱克多巴胺的荧光偏振免疫分析。
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-11-01
E A Zvereva, N A Shpakova, A V Zherdev, L Kiu, C Xu, S A Eremin, B B Dzantiev
{"title":"[Fluorescence polarization immunoassay of ractopamine].","authors":"E A Zvereva,&nbsp;N A Shpakova,&nbsp;A V Zherdev,&nbsp;L Kiu,&nbsp;C Xu,&nbsp;S A Eremin,&nbsp;B B Dzantiev","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A technique was developed for fluorescence polarization immunoassay (FPIA) of ractopamine, a toxic low molecular weight nonsteroidal growth regulator belonging to the most controlled contaminants of food products of animal origin. The assay is based on the competition between a sample containing ractopamine and ractopamine–fluorophore conjugate for binding to antibodies. The competition is monitored via changes in the degree of fluorescence polarization for plane-polarized excitation light, which differs for the free and antibody-bound forms of the conjugate. The optimal assay conditions were established, ensuring a high accuracy and minimal detection limit. The developed assay demonstrated a detection limit of 1 ng/mL and a range of detectable concentrations of 2.3–50 ng/mL, which met the requirements of sanitary control. The duration of the analysis was 10 min. The possible application of the developed FPIA was demonstrated with testing of turkey meat. The speed and simplicity of the proposed assay define its efficiency as a screening tool for safety of foods.</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35891168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Oil degradation by basidiomycetes in soil and peat at low temperatures]. [低温下担子菌在土壤和泥炭中的石油降解]。
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-11-01
N A Kulikova, O I Klein, D V Pivchenko, E O Landesman, N N Pozdnyakova, O V Turkovskaya, B Ts Zaichik, A O Ruzhitskii, O V Koroleva
{"title":"[Oil degradation by basidiomycetes in soil and peat at low temperatures].","authors":"N A Kulikova,&nbsp;O I Klein,&nbsp;D V Pivchenko,&nbsp;E O Landesman,&nbsp;N N Pozdnyakova,&nbsp;O V Turkovskaya,&nbsp;B Ts Zaichik,&nbsp;A O Ruzhitskii,&nbsp;O V Koroleva","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A total of 17 basidiomycete strains causing white rot and growing on oil-contaminated substrates have been screened. Three strains with high (Steccherinum murashkinskyi), average (Trametes maxima), and low (Pleurotus ostreatus) capacities for the colonization of oil-contaminated substrates have been selected. The potential for degrading crude oil hydrocarbons has been assessed with the use of fungi grown on nonsterile soil and peat at low temperatures. Candida sp. and Rhodococcus sp. commercial strains have been used as reference organisms with oil-degrading ability. All microorganisms introduced in oil-contaminated soil have proved to be ineffective, whereas the inoculation of peat with basidiomycetes and oil-degrading microorganisms accelerated the destruction of oil hydrocarbons. The greatest degradation potential of oil-aliphatic hydrocarbons has been found in S. murashlinskyi. T. maxima turned out to be the most successful in degrading aromatic hydrocarbons. It has been suggested that aboriginal microflora contributes importantly to the effectiveness of oil-destructing microorganisms. T. maxima and S. murashkinskyi strains are promising for further study as oil-oxidizing agents during bioremediation of oil-contaminated peat soil under conditions of low temperatures.</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35892226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[VapC toxin inhibition as a method for prevention of the formation of resting forms of mycobacteria]. [VapC毒素抑制作为预防分枝杆菌静止形态形成的方法]。
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-11-01
O I Demidenok, M S Shumkov, A V Goncharenko
{"title":"[VapC toxin inhibition as a method for prevention of the formation of resting forms of mycobacteria].","authors":"O I Demidenok,&nbsp;M S Shumkov,&nbsp;A V Goncharenko","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>It has been shown that inactivation of the VapC toxin from the VapВС toxin-antitoxin system prevents mycobacterial cells from transitioning to an ovoid state that meets the criteria of dormancy. The results indicate a potential target for medicines that prevent the development of latent tuberculosis infection and provide a basis to obtain bacterial cells for the testing of compounds that are active towards dormant forms of mycobacteria.</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35890718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[New strains of basidiomycetes that produce bioethanol from lignocellulose biomass]. [从木质纤维素生物质中生产生物乙醇的担子菌的新菌株]。
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-11-01
E Yu Kozhevnikova, D A Petrova, D S Kopitsyn, A A Nivikov, A V Shnyreva, A V Barkov, V A Vinokurov
{"title":"[New strains of basidiomycetes that produce bioethanol from lignocellulose biomass].","authors":"E Yu Kozhevnikova,&nbsp;D A Petrova,&nbsp;D S Kopitsyn,&nbsp;A A Nivikov,&nbsp;A V Shnyreva,&nbsp;A V Barkov,&nbsp;V A Vinokurov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Sixty six isolates were screened for ability of bioethanol production; dynamics of product accumulation and substrate utilization were investigated for two selected strains Trametes hirsuta MT-24.24 and Trametes versicolor IT-1. The strains’ efficiency was evaluated as bioethanol production by 1 g biomass. Strain T. versicolor IT-1 producing over 33 g/L of the ethanol for 9 d was selected. Direct conversion of Na-carboxymethyl cellulose, microcrystalline cellulose and straw was shown with ethanol yields of 2.1, 1.6 and 1.7 g/L, respectively, for 9 d fermentation time.</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35892228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Catalytic properties of lipase adsorbed on nanocarbon-containing mesoporous silica in esterification and transesterification reactions]. [含纳米碳介孔二氧化硅吸附脂肪酶在酯化和酯交换反应中的催化性能]。
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-11-01
G A Kovalenko, L V Perminova, T V Chuenko, N A Rudina
{"title":"[Catalytic properties of lipase adsorbed on nanocarbon-containing mesoporous silica in esterification and transesterification reactions].","authors":"G A Kovalenko,&nbsp;L V Perminova,&nbsp;T V Chuenko,&nbsp;N A Rudina","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Nanocarbon-containing mesoporous silica covered with a varying amounts of nanostructured carbon of different morphologies were used as supports to immobilize Thermomyces lanuginosus lipase. The catalytic properties of the prepared biocatalysts were studied in both the transesterification of vegetable (linseed) oil in the presence of ethyl acetate and the esterification of the fatty acid (capric C10:0) in the presence of secondary (isopropyl or isoamyl) alcohols. The physico-chemical characteristics, such as the amount of adsorbed lipase, its specific activity, and the dependence of the activity and stability of the prepared biocatalysts on the support type were evaluated. The Michaelis-Menten kinetics was studied in the esterification of capric acid with isoamyl alcohol. The prepared biocatalysts were shown to retain up to 90% activity for >1000 h in the synthesis of isoamyl caprate. The half-time of the biocatalysts inactivation in the transesterification of linseed oil was found to be more than 700 h at 40°C.</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35890717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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