[Inhibition of IAA oxidase activity of wheat anionic peroxidase by chitooligosaccharides].

I V Maksimov, Z R Usupova, E A Cherepanova, R M Khairulin, V A Vakhitov
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Abstract

The study demonstrated that chitooligosaccharides with a molecular weight of 5–10 kDa and a degree of acetylation of 65% exhibited an auxin-like effect in wheat plants and also played an important role in regulating the activity of polysaccharide (chitin)–specific anion isoenzymes of peroxidase oxidizing indole acetic acid. Changes in the kinetic parameters of the interaction of the wheat anionic chitin-specific peroxidase with pI ∼3.5 with chitin oligomers in the presence of indoleacetic acid were pH-depended and indicated that chitooligosaccharides significantly impair the ability of the enzyme for oxidation at pH levels of 4.2 and 6.0. It can be assumed that chitooligosaccharides not only induce protective plant systems but also increase the accumulation of auxin in plant tissues, thus adversely affecting a number of components of the plant protective system against pathogens.

[壳寡糖对小麦阴离子过氧化物酶IAA氧化酶活性的抑制作用]。
研究表明,分子量为5 ~ 10 kDa、乙酰化程度为65%的壳寡糖在小麦植株中表现出类似生长素的作用,并在调节多糖(几丁质)特异性过氧化物酶氧化吲哚乙酸阴离子同工酶的活性中发挥重要作用。在吲哚乙酸的存在下,pI ~ 3.5的小麦阴离子几丁质特异性过氧化物酶与几丁质低聚物相互作用的动力学参数的变化是pH依赖的,表明壳寡糖在4.2和6.0的pH水平下显著损害了酶的氧化能力。可以认为,壳寡糖不仅可以诱导植物保护系统,还可以增加植物组织中生长素的积累,从而对植物保护系统的许多组成部分产生不利影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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