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[Thermotolerant oil-degrading bacteria isolated from soil and water of geographically distant regions]. [从地理位置遥远的土壤和水中分离出的耐高温石油降解细菌]。
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-07-01
Ya A Delegan, A A Vetrova, V N Akimov, M A Titok, A E Filonov, A M Boronin
{"title":"[Thermotolerant oil-degrading bacteria isolated from soil and water of geographically distant regions].","authors":"Ya A Delegan,&nbsp;A A Vetrova,&nbsp;V N Akimov,&nbsp;M A Titok,&nbsp;A E Filonov,&nbsp;A M Boronin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Oil-degrading bacteria were isolated from soil and water samples taken in Russia, Kazakhstan, and the Antarctic; 13 of 86 strains proved to be thermotolerant. These bacteria utilized crude oil at 45–50°C; their growth optimum (35–37°C) and range (20–53°C) differ from those of mesophilic bacteria. Thermotolerant strains were identified as representatives of the genera Rhodococcus and Gordonia. It was shown that their ability to degrade petroleum products does not differ at 24 and 45°C. The strains Rhodococcus sp. Par7 and Gordonia sp. 1D utilized 14 and 20% of the oil, respectively, in 14 days at 45°C. All of the isolated thermotolerant bacteria grew in a medium containing 3% NaCl; the medium for the strains Gordonia amicalis 1B and Gordonia sp. 1D contained up to 10% NaCl. The bacteria G. amicalis and Rhodococcus erythropolis were able to utilize crude oil and individual hydrocarbons at higher (up to 50°C) temperatures.</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35890456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Production of humanized F(ab’)2 fragment of rabies blocking antibodies in Pichia pastoris yeast]. [毕赤酵母人源化狂犬病阻断抗体F(ab’)2片段的制备]。
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-07-01
T A Yagudin, E V Klyatchko, S S Zatsepin, E V Morozkina, S V Benevolensky, O B Shemchukova, L P Pozdnyakova, O N Solopova, P G Sveshnikov
{"title":"[Production of humanized F(ab’)2 fragment of rabies blocking antibodies in Pichia pastoris yeast].","authors":"T A Yagudin,&nbsp;E V Klyatchko,&nbsp;S S Zatsepin,&nbsp;E V Morozkina,&nbsp;S V Benevolensky,&nbsp;O B Shemchukova,&nbsp;L P Pozdnyakova,&nbsp;O N Solopova,&nbsp;P G Sveshnikov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The yeast strain Pichia pastoris, a producer of humanized F(ab’)2 fragments of rabies-blocking antibodies, has been obtained. Human chaperone BiP coexpression caused a twofold increase of the immunoglobulins secretion level. The use of Fos and Jun zippers in the composition of heavy chains facilitated the dimerization of F(ab’)2 fragments of the shared pool of secreted immunoglobulins up to 75%.</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35890563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Dihydroquercetin polymerization using laccase immobilized into an ionic liquid]. 用固定在离子液体中的漆酶聚合双氢槲皮素。
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-07-01
M E Khlupova, K V Lisitskaya, A H Amandusova, G P Shumakovich, I S Vasil'eva, E A Zaitseva, O V Morozova, A I Yaropolov
{"title":"[Dihydroquercetin polymerization using laccase immobilized into an ionic liquid].","authors":"M E Khlupova,&nbsp;K V Lisitskaya,&nbsp;A H Amandusova,&nbsp;G P Shumakovich,&nbsp;I S Vasil'eva,&nbsp;E A Zaitseva,&nbsp;O V Morozova,&nbsp;A I Yaropolov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>It was shown that the laccase (LC) included into hydrophobic ionic liquid (IL) can be reused for the biotransformation of dihydroquercetin (DHQ). The physicochemical characteristics of DHQ oligomers synthesized using LC/IL did not differ from the characteristics of the oligomers obtained with native laccase. The synthesized oligomers have a number average molecular weight of 1050 g/mol and a polydispersity index of 1.41. Oligomers possess higher antioxidant activity than the monomer.</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35889870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Impossible mechanisms of germatranol influence on the thermal stability of wheat germs]. [酵母醇影响小麦胚芽热稳定性的可能机制]。
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-07-01
A M Shigarova, O I Grabelnych, V P Baryshok, G B Borovskii
{"title":"[Impossible mechanisms of germatranol influence on the thermal stability of wheat germs].","authors":"A M Shigarova,&nbsp;O I Grabelnych,&nbsp;V P Baryshok,&nbsp;G B Borovskii","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The influence of biologically active substance germatranol in low and very low doses on the respiration rate and the content of reactive oxygen species (ROS) in wheat roots under conditions of high stress was investigated. The results showed that solutions of the studied substance in concentrations of from 10–5 M to 10–10 M (depending on the temperature) increase the thermotolerance of wheat germs and affect the ROS content and respiration rate. It is assumed that germatranol can have a positive impact on plant growth and development and act as antioxidants in plant cells.</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35890458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The effect of 3,5-dicarbomethoxyphenylbiguanide on the activity of antioxidant enzymes]. [3,5-二碳甲氧基苯基双胍对抗氧化酶活性的影响]。
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-07-01
O A Safonova, T N Popova, E D Krylskiy, A A Agarkov, K K Shulgin, E M Kirilova, E S Tanygina
{"title":"[The effect of 3,5-dicarbomethoxyphenylbiguanide on the activity of antioxidant enzymes].","authors":"O A Safonova,&nbsp;T N Popova,&nbsp;E D Krylskiy,&nbsp;A A Agarkov,&nbsp;K K Shulgin,&nbsp;E M Kirilova,&nbsp;E S Tanygina","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The effect of 3,5-dicarbomethoxyphenylbiguanide, which was selected with the Prediction of Activity Spectra of Substances (PASS) computer program, on the activity of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, and glutathione transferase in the heart and the blood serum of rats with experimental rheumatoid arthritis was investigated. The studied parameters changed towards control values when the tested compound was injected in animals with the pathology. These results can be explained by the cardioprotective and antioxidant activity of the compound. The data obtained during the study may be used for the development of new preventive and therapeutic agents for the treatment of the rheumatoid arthritis.</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35890462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Neutralization of anticoagulant activity of heparin by N-[(2-hydroxy-3-trimethylammonium) propyl] chloride derivatives of chitosan]. [壳聚糖N-[(2-羟基-3-三甲基铵)丙基]氯衍生物中和肝素抗凝血活性]。
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-07-01
B Ts Shagdarova, N N Drozd, A V Il'ina, Yu S Logviniva, V P Varlamov
{"title":"[Neutralization of anticoagulant activity of heparin by N-[(2-hydroxy-3-trimethylammonium) propyl] chloride derivatives of chitosan].","authors":"B Ts Shagdarova,&nbsp;N N Drozd,&nbsp;A V Il'ina,&nbsp;Yu S Logviniva,&nbsp;V P Varlamov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Alkylated derivatives of low molecular weight chitosan with different substitution degrees of 98, 40, and 9% (I, II, and III respectively) have been synthesized. The structure of the obtained derivatives was defined by spectral assays (IR-spectroscopy and proton magnetic resonance). Chitosan derivatives were characterized with positive zeta-potential (33–51 mV) and solubility from 2 to 100 mg/mL in pH 7.4 and 25°C. It was shown that, at a concentration of 0.0014–0.0029 mg/mL, derivative I, as well as protamine sulfate, could be used to neutralize the anticoagulant activity of unfractionated or low molecular weight heparin. At a concentration of 0.0029–0.58 mg/mL, derivative I enhanced platelet aggregation, which would be necessary when hemostatic compounds or materials were used. Derivatives II and III enhanced platelet aggregation to a lesser extent.</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35890464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Physicochemical and catalytic properties of NAD+- dependent malate dehydrogenase isoforms from maize mesophyll0. 玉米叶肉中NAD+依赖性苹果酸脱氢酶异构体的理化和催化性质[j]。
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-07-01
A T Eprintsev, M O Gataullina, M S Lyashchenko
{"title":"[Physicochemical and catalytic properties of NAD+- dependent malate dehydrogenase isoforms from maize mesophyll0.","authors":"A T Eprintsev,&nbsp;M O Gataullina,&nbsp;M S Lyashchenko","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Malate dehyrogenase isoforms (46- and 70-fold purifications) with specific activities of the 640 and 990 U/mg protein were obtained in an electrophoretically homogeneous state from maize mesophyll. The physicochemical and catalytic properties of these isoforms were studied. The molecular weight and the Michaelis constants were determined; the effect of hydrogen ions on the forward and reverse MDH reaction was studied. The results of SDS-PAGE demonstrated that malate dehydrogenase isoforms have an oligomeric structure comprised of identical subunits. The first isoform with a molecular weight of 126.58 kDa is tetramer, and the second isoform with a molecular weight of 63.3 is dimer.</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35890561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Biooxidation of gold-bearing sulfide ore and subsequent biological treatment of cyanidation residues]. [含金硫化矿的生物氧化及氰化残留物的后续生物处理]。
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-07-01
A T Kanaev, A G Bulaev, G V Semenchenko, Z K Kanaeva, A A Shilmanova
{"title":"[Biooxidation of gold-bearing sulfide ore and subsequent biological treatment of cyanidation residues].","authors":"A T Kanaev,&nbsp;A G Bulaev,&nbsp;G V Semenchenko,&nbsp;Z K Kanaeva,&nbsp;A A Shilmanova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The percolation biooxidation parameters of ore from the Bakyrchik deposit were studied. An investigation of the technological parameters (such as the concentration of leaching agents, irrigation intensity, and pauses at various stages of the leaching) revealed the optimal mode for precious metal extraction. The stages of the ore processing were biooxidation, gold extraction by cyanidation or thiosulfate leaching, and biological destruction of cyanide. The gold and silver recovery rates by cyanidation were 64.0 and 57.3%, respectively. The gold and silver recovery rates by thiosulfate leaching were 64.0 and 57.3%, respectively. Gold and silver recovery rates from unoxidized ore (control experiment) by cyanidation were 20.9 and 26.8%, respectively. Thiosulfate leaching of unoxidized ore allowed the extraction of 38.8 and 24.2% of the gold and silver, respectively. Cyanidation residues were treated with bacteria of the genus Alcaligenes in order to destruct cyanide.</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35890457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Whole-cell bacterial biosensors for the detection of aromatic hydrocarbons and their chlorinated derivatives]. [用于检测芳香烃及其氯化衍生物的全细胞细菌生物传感器]。
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-07-01
E G Plotnikova, E S Shumkova, M S Shumkov
{"title":"[Whole-cell bacterial biosensors for the detection of aromatic hydrocarbons and their chlorinated derivatives].","authors":"E G Plotnikova,&nbsp;E S Shumkova,&nbsp;M S Shumkov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The review summarizes the data on new directions in biosensor technologies based on whole bacterial cells. Biosensors for the monitoring of mono(poly)aromatic hydrocarbons and their chlorinated derivatives, which are constructed with genetically modified bacterial cells bearing a reporter gene fusion, are considered. The operating principle of these biosensors is based on the expression of reporter genes (luc, lux, gfp, rfp) under the control of a promoter and a regulator that specifically respond to a detected compound.</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35890559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Immunodetection of bacteriophages by a piezoelectric resonator with lateral electric field]. 基于侧电场的压电谐振器的噬菌体免疫检测。
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-07-01
O I Gulii, B D Zaitsev, A M Shikhabudinov, A A Teplykh, I A Borodina, S A Pavlii, O S Larionova, A S Fomin, S A Staroverov, L A Dykman, O V Ignatov
{"title":"[Immunodetection of bacteriophages by a piezoelectric resonator with lateral electric field].","authors":"O I Gulii,&nbsp;B D Zaitsev,&nbsp;A M Shikhabudinov,&nbsp;A A Teplykh,&nbsp;I A Borodina,&nbsp;S A Pavlii,&nbsp;O S Larionova,&nbsp;A S Fomin,&nbsp;S A Staroverov,&nbsp;L A Dykman,&nbsp;O V Ignatov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>It has been demonstrated that electroacoustic analysis with polyclonal antibodies can be used for bacteriophage detection. The frequency dependences of the real and imaginary parts of electrical impedance of a resonator with a viral suspension with antibodies were shown to be essentially different from the dependences of a resonator with control viral suspension without antibodies. It was shown that ΦAl-Sp59b bacteriophages were detected with the use of antibodies in the presence of foreign virus particles. The ΦAl-Sp59b bacteriophage content in the analyzed suspension was ~1010–106 phages/mL; the time of analysis was no more than 5 min. The optimally informative parameter for obtaining reliable information was the change in the real or imaginary part of electrical impedance at a fixed frequency near the resonance upon the addition of specific antibodies to the analyzed suspension. It was demonstrated that the interaction between bacteriophages and antibodies can be recorded, offering good prospects for the development of a biological sensor for liquid-phase identification and virus detection.</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35889871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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