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[Effect of N-phenyl-2-naphthylamine on activity of adenylate cyclase signal system components and virulence of bacterial phytopathogens and mutualists]. [N-苯基-2-萘胺对腺苷酸环化酶信号系统成分活性以及细菌性植物病原菌和互生菌毒力的影响]。
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-05-01
L A Lomovatskaya, L E Makarova, O V Kuzakova, A S Romanenko, A M Goncharova
{"title":"[Effect of N-phenyl-2-naphthylamine on activity of adenylate cyclase signal system components and virulence of bacterial phytopathogens and mutualists].","authors":"L A Lomovatskaya, L E Makarova, O V Kuzakova, A S Romanenko, A M Goncharova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The effect of N-phenyl-2-naphthylamine, negative allelochemical isolated from the exudates of roots of pea (Pisum sativum L.), on the growth and activity of the adenylate cyclase signal system and virulence factors of the bacteria Rhizobium leguminosarum bv. viciae and Pseudomonas siringae pv. pisi was studied. It was demonstrated that N-phenyl-2-naphthylamine at a physiological concentration nonspecifically inhibited the growth of these bacteria in both planktonic cultures and biofilms. One of the reasons for this phenomenon is the reduction of intra- and extracellular concentrations of cAMP due to greater activation of phosphodiesterase, which disrupts cAMP, in comparison to soluble adenylyl cyclase, which synthesizes it. At the same time, N-phenyl-2-naphthylamine did not affect activity of either membrane-bound adenylyl cyclase or bacterial virulence factors.</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":"52 3","pages":"306-11"},"PeriodicalIF":0.0,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35886308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[A search for microscopic fungi with directed hydroxylase activity for the synthesis of steroid drugs]. [对合成类固醇药物的具有定向羟化酶活性的微观真菌的研究]。
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-05-01
N V Karpova, V A Andryushina, T S Stytsenko, A V Druzhinina, T D Feofanova, A V Kurakov
{"title":"[A search for microscopic fungi with directed hydroxylase activity for the synthesis of steroid drugs].","authors":"N V Karpova,&nbsp;V A Andryushina,&nbsp;T S Stytsenko,&nbsp;A V Druzhinina,&nbsp;T D Feofanova,&nbsp;A V Kurakov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The hydroxylase activities of new strains such as Curvularia lunata, C. geniculata, C. eragrostidis, C. prasadii, Ulocladium botrytis, Alternaria tenuis, and Fusarium oxysporum toward three steroid substrates, namely, androstenedione (AD), cortexolone (S), and dehydroepiandrosterone acetate (DAA), were characterized. The 9α-hydroxylase activity of C. lunata 1011 cells against S to form 9α-hydroxy-S was shown for the first time. It was found that C. geniculata 837 and F. oxysporum 11dn1 strains can hydroxylate substrates to form pharmacologically promising 7α-hydroxysteroids. C. geniculata 837 cells selectively hydroxylate AD, resulting in 7α-hydroxytestosterone, whereas F. oxysporum 11dn1 leads to the transformation of DAA to 7α-hydroxydehydroepiandrosterone.</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":"52 3","pages":"324-32"},"PeriodicalIF":0.0,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35886311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Homo- and heterologous reporter proteins for evaluation of promoter activity in Methylomicrobium alcaliphilum 20Z]. [评价甲基微生物alcaliphilum 20Z启动子活性的同源和异源报告蛋白]。
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-05-01
I I Mustakhimov, S Y But, A S Reshetnikov, V N Khmelenina, Y A Trotsenko
{"title":"[Homo- and heterologous reporter proteins for evaluation of promoter activity in Methylomicrobium alcaliphilum 20Z].","authors":"I I Mustakhimov,&nbsp;S Y But,&nbsp;A S Reshetnikov,&nbsp;V N Khmelenina,&nbsp;Y A Trotsenko","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A number of vectors were constructed based on the plasmid from the broad range of pMHA200 hosts. Also, the expression of some key genes of the haloalkalitolerant methanotroph Methylomicrobium alcaliphilum 20Z was studied. The activities of the promoter regions of genes for hexulose phosphate synthase, glutamine synthetase, and glucokinase, as well as the promoter of the ectABC-ask operon, which encodes enzymes for osmoprotectant ectoine biosynthesis, were evaluated with the use of the gfp gene; the evaluation was proven to be ineffective. Conversely, glucokinase and a heterologous enzyme of chloramphenicol acetyltransferase were useful for the evaluation of promoter activity. In M. alcaliphilum 20Z cells, the expression level of chloramphenicol acetyltransferase transcribed from the methanol dehydrogenase promoter was higher as compared with that of glucokinase. This seems to be due to a regulatory mechanism for homologous protein expression. The introduction of a synthetic nucleotide sequence forming the secondary structure in the 5′ untranslated region of the glucokinase mRNA resulted in an increase of this enzyme level. This is the first attempt to use M. alcaliphilum 20Z for homo- and heterologous protein expression.</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":"52 3","pages":"279-86"},"PeriodicalIF":0.0,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35887433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Inhibition of oxidation of unsaturated fatty acid methyl esters by essential oils]. [精油对不饱和脂肪酸甲酯氧化的抑制作用]。
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-05-01
T A Misharina, E S Alinkina, A K Vorobjeva, M B Terenina, N I Krikunova
{"title":"[Inhibition of oxidation of unsaturated fatty acid methyl esters by essential oils].","authors":"T A Misharina,&nbsp;E S Alinkina,&nbsp;A K Vorobjeva,&nbsp;M B Terenina,&nbsp;N I Krikunova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The essential oils from 16 various spice plants were studied as natural antioxidants for the inhibition of autooxidation of polyunsaturated fatty acids methyl esters isolated from linseed oil. The content of methyl oleate, methyl linoleate, and methyl linolenoate after 1, 2, and 4 months of autooxidation were used as criteria to estimate the antioxidant efficiencies of essential oils. In 4 months, 92% of the methyl linolenoate and 79% of the methyl linoleate were oxidized in a control sample of a model system. It was found that the most effective antioxidants were essential oils from clove bud, cinnamon leaves, and oregano. They inhibited autooxidation of methyl linolenoate by 76–85%. The antioxidant properties of these essential oils were due to phenols— eugenol, carvacrol, and thymol. Essential oil from coriander did not contain phenols, but it inhibited methyl linolenoate oxidation by 38%. Essential oils from thyme, savory, mace, lemon, and tea tree inhibited methyl linolenoate oxidation by 17–24%. The other essential oils had no antioxidant properties.</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":"52 3","pages":"339-45"},"PeriodicalIF":0.0,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35886312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cloning, purification and characterization of a cellulase-free xylanase from Geobacillus thermodenitrificans AK53. 热反硝化地杆菌AK53无纤维素酶木聚糖酶的克隆、纯化及特性研究
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-05-01
M Irfan, H I Guler, A O Belduz, A A Shah, S Canakci
{"title":"Cloning, purification and characterization of a cellulase-free xylanase from Geobacillus thermodenitrificans AK53.","authors":"M Irfan,&nbsp;H I Guler,&nbsp;A O Belduz,&nbsp;A A Shah,&nbsp;S Canakci","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Geobacillus thermodenitrificans AK53 xyl gene encoding xylanase was isolated, cloned and expressed in Escherichia coli. After purifying recombinant xylanase from G. thermodenitrificans AK53 (GthAK53Xyl) to homogeneity by ammonium sulfate precipitation and ion exchange chromatography, biochemical properties of the enzyme were determined. The kinetic studies for GthAK53Xyl showed K M value to be 4.34 mg/mL (for D-xylose) and V max value to be 2028.9 μmoles mg–1 min–1. The optimal temperature and pH for enzyme activity were found out to be 70°C and 5.0, respectively. The expressed protein showed the highest sequence similarity with the xylanases of G. thermodenitrificans JK1 (JN209933) and G. thermodenitrificans T-2 (EU599644). Metal cations Mg2+ and Mn2+ were found to be required for the enzyme activity, however, Co2+, Hg2+, Fe2+ and Cu2+ ions caused inhibitor effect on it. GthAK53Xyl had no cellulolytic activity and degraded xylan in an endo-fashion. The action of the enzyme on xylan from oat spelt produced xylobiose and xylopentose. The reported results are suggestive of a xylanase exhibiting desirable kinetics, stability parameters and metal resistance required for the efficient production of xylobiose at industrial scale.</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":"52 3","pages":"296-305"},"PeriodicalIF":0.0,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35886307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Bioregulator from rat liver tissue]. [来自大鼠肝组织的生物调节剂]。
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-05-01
D I Maltsev, V P Yamskova, A P Il'na, B B Beresin, I A Yamskov
{"title":"[Bioregulator from rat liver tissue].","authors":"D I Maltsev,&nbsp;V P Yamskova,&nbsp;A P Il'na,&nbsp;B B Beresin,&nbsp;I A Yamskov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>It has been shown that the membranotropic homeostatic tissue-specific bioregulator isolated from rat liver tissue contains a nanosized peptide-protein complex consisting of low-molecular peptides (1–6.5 kDa) and a protein from the serum albumin family. This bioregulator modulated the peptide biological activity and determined the tissue specificity.</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":"52 3","pages":"346-50"},"PeriodicalIF":0.0,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35887323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Plant-Producers Of Recombinant Cytokines (Review)]. 重组细胞因子的植物生产者(综述)。
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-03-01
M S Burlakovskii, V V Yemel'yanov, L A Lutova
{"title":"[Plant-Producers Of Recombinant Cytokines (Review)].","authors":"M S Burlakovskii,&nbsp;V V Yemel'yanov,&nbsp;L A Lutova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Cytokines are a family of signaling polypeptides involved in cell-cell interactions in the process of the immune response, as well as in the regulation of a number of normal physiological functions. Cytokines are used in medicine for the treatment of cancer, immune disorders, viral infections, and other socially significant diseases, but the extent of their use is limited by the high production cost of the active agent. The development of this area of pharmacology is associated with the success of genetic engineering, which allows the production of significant amounts of protein by transgenic organisms. The review discusses the latest advances in the production of various cytokines with the use of genetically modified plants.</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":"52 2","pages":"149-67"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34442857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Secretion of Phenolic Compounds into Root Exudates of Pea Seedlings upon Inoculation with Rhizobium leguminosarum bv. viceae or Pseudomonas siringae pv. Pisi]. [接种豆荚根瘤菌 v. viceae 或 Pseudomonas siringae pv. Pisi 后豌豆幼苗根部渗出物中酚类化合物的分泌]。
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-03-01
L E Makarova, L V Dudareva, I G Petrova, G G Vasil'eva
{"title":"[Secretion of Phenolic Compounds into Root Exudates of Pea Seedlings upon Inoculation with Rhizobium leguminosarum bv. viceae or Pseudomonas siringae pv. Pisi].","authors":"L E Makarova, L V Dudareva, I G Petrova, G G Vasil'eva","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The content of apigenin, naringenin, pisatin, dibutyl-ortho-phthalate, and N-phenyl-2-naphthyl-amine were assayed in root exudates of pea (Pisum sativum L.) seedlings one day after their inoculation with Rhizobium leguminosarum, bv. viceae or Pseudomonas siringae pv. pisi, which represent, respectively, mutualistic and antagonistic strategies of interaction with a host plant. After inoculation with either bacteria, the concentrations of apigenin and pisatin in the root exudates were equal, whereas the concentrations of naringenin and N-phenyl-2-naphthylamine were different and those of dibutyl-o-phthalate were unchanged. A certain role is suggested for the phenolic compounds in an accomplishment of symbiotic relations of bacteria with a host plant.</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":"52 2","pages":"217-22"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34442862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Antibacterial Activity of Alkylated and Acylated Derivatives of Low-Molecular Weight Chitosan]. [低分子量壳聚糖烷基化和酰化衍生物的抑菌活性]。
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-03-01
B Ts Shagdarova, A V Il'ina, V P Varlamov
{"title":"[Antibacterial Activity of Alkylated and Acylated Derivatives of Low-Molecular Weight Chitosan].","authors":"B Ts Shagdarova,&nbsp;A V Il'ina,&nbsp;V P Varlamov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A number of alkylated (quaternized) and acylated derivatives of low-molecular weight chitosan were obtained. The structure and composition of the compounds were confirmed by the results of IR and PMR spectroscopy, as well as conductometric titration. The effect of the acyl substituent and the degree of substitution of N-(2-hydroxy-3-trimethylammonium) with the propyl fragment appended to amino groups of the C2 atom of polymer chains on antibacterial activity against typical representatives of gram-positive and gram-negative microorganisms (Staphylococcus epidermidis and Escherichia coli) was studied. The highest activity was in the case of N-[(2-hydroxy-3-trimethylammonium)propyl]chitosan chloride with the maximal substitution (98%). The minimal inhibitory concentration of the derivative was 0.48 µg/mL and 3.90 µg/mL for S. epidermis and E. coli, respectively.</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":"52 2","pages":"237-41"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34615165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Expression Of DNA-Encoded Antidote to Organophosphorus Toxins in the Methylotrophic Yeast Pichia Pastoris]. [甲基营养酵母毕赤酵母中有机磷毒素解毒剂的dna编码表达]。
Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-03-01
S S Terekhov, T V Bobik, Yu A Mokrushina, A V Stepanova, N M Aleksandrova, I V Smirnov, A A Belogurov, N A Ponomarenko, A G Gabibov
{"title":"[Expression Of DNA-Encoded Antidote to Organophosphorus Toxins in the Methylotrophic Yeast Pichia Pastoris].","authors":"S S Terekhov,&nbsp;T V Bobik,&nbsp;Yu A Mokrushina,&nbsp;A V Stepanova,&nbsp;N M Aleksandrova,&nbsp;I V Smirnov,&nbsp;A A Belogurov,&nbsp;N A Ponomarenko,&nbsp;A G Gabibov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A platform for the cloning and expression of active human butyrylcholinesterase (BuChE) in the yeast Pichia pastoris is first presented. Genetic constructs for BuChE gene expression, separately and in conjunction with a proline-rich peptide called proline-rich attachment domain (PRAD), are based on the vector pPICZαA. It is shown that the highest level of production is achieved in the expression of a BuChE gene without PRAD pPICZαA. It is found that one can obtain up to 125 mg of active enzyme from 1 L of culture medium at an optimal pH environment (pH 7.6), an optical seed culture density of 3 o.u., and an optimum methanol addition mode of (0.5% methanol in the first day and 0.2% thereafter from the second day).</p>","PeriodicalId":20415,"journal":{"name":"Prikladnaia biokhimiia i mikrobiologiia","volume":"52 2","pages":"184-92"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34442860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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