Phytopathology最新文献_第7页

Application of Loop-Mediated Isothermal Amplification in Plant Pathogen Detection. 环介导等温扩增（LAMP）在植物病原体检测中的应用。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2025-01-01 Epub Date: 2025-01-09 DOI: 10.1094/PHYTO-10-23-0391-KC

Liuliu Yang, Yu Sun, Lin Sun, Zehao Wang, Jie Feng, Yue Liang

引用次数: 0

Field-Scale Gene Flow of Fungicide Resistance in Pyrenophora teres f. teres and the Effect of Selection Pressure on the Population Structure. 赤霉病菌对杀真菌剂抗性的田间规模基因流动以及选择压力对种群结构的影响。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2025-01-01 Epub Date: 2025-01-10 DOI: 10.1094/PHYTO-10-23-0378-KC

Leon M Hodgson, Francisco J Lopez-Ruiz, Mark R Gibberd, Geoff J Thomas, Ayalsew Zerihun

{"title":"Field-Scale Gene Flow of Fungicide Resistance in Pyrenophora teres f. teres and the Effect of Selection Pressure on the Population Structure.","authors":"Leon M Hodgson, Francisco J Lopez-Ruiz, Mark R Gibberd, Geoff J Thomas, Ayalsew Zerihun","doi":"10.1094/PHYTO-10-23-0378-KC","DOIUrl":"10.1094/PHYTO-10-23-0378-KC","url":null,"abstract":"The effectiveness of fungicides to control foliar fungal crop diseases is being diminished by the increasing spread of resistance to fungicides. One approach that may help to maintain efficacy is remediation of resistant populations by sensitive ones. However, the success of such approaches can be compromised by re-incursion of resistance through aerial spore dispersal, although knowledge of localized gene flow is lacking. Here, we report on a replicated mark-release-recapture field experiment with several treatments set up to study spore-dispersal-mediated gene flow of a mutated allele that confers demethylase inhibitor resistance in Pyrenophora teres f. teres (Ptt). Artificial inoculation of the host, barley (Hordeum vulgare), was successful across the 12-ha trial, where the introduced sensitive and resistant populations were, respectively, 6- and 13-fold the DNA concentration of the native Ptt population. Subsequent disease pressure remained low, which hampered spread of the epidemic to such an extent that gene flow was not detected at, or beyond, 2.5 m from source points. In the absence of gene flow, plots were assessed for treatment effects; fungicide applied to populations that contained 14.3% of allele mutation increased in frequency to 24.5%, whereas sensitive populations had no change in structure. Untreated controls of the native Ptt population remained genetically stable, yet untreated controls that were inoculated with sensitive Ptt had half the resistance frequency of the native population structure. The trial demonstrates the potential for management to remediate fungicide-resistant pathogen populations, where localized gene flow is minimal, to safeguard chemical crop protection into the future.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"85-96"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142366279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Characterization of Fusarium Isolates from Upland Cotton Roots in Uzbekistan and Whole-Genome Comparison with Isolates from the United States. 乌兹别克斯坦陆地棉根部镰刀菌分离物的分子特征以及与美国分离物的全基因组比较。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2025-01-01 Epub Date: 2025-01-13 DOI: 10.1094/PHYTO-04-24-0152-R

Timothy O Jobe, Ibrokhim Y Abdurakhmonov, Mauricio Ulloa, Mohamed Fokar, Zabardast T Buriev, Shukhrat E Shermatov, Abdusalom K Makamov, Dilshod E Usmanov, Mukhtor M Darmanov, Kirk Broders, Margaret L Ellis

{"title":"Molecular Characterization of Fusarium Isolates from Upland Cotton Roots in Uzbekistan and Whole-Genome Comparison with Isolates from the United States.","authors":"Timothy O Jobe, Ibrokhim Y Abdurakhmonov, Mauricio Ulloa, Mohamed Fokar, Zabardast T Buriev, Shukhrat E Shermatov, Abdusalom K Makamov, Dilshod E Usmanov, Mukhtor M Darmanov, Kirk Broders, Margaret L Ellis","doi":"10.1094/PHYTO-04-24-0152-R","DOIUrl":"10.1094/PHYTO-04-24-0152-R","url":null,"abstract":"Fusarium oxysporum f. sp. vasinfectum (FOV) is a significant cotton (Gossypium spp.) pathogen causing vascular wilt, browning of the vascular tissues, and plant death in the most severe cases. This global disease is responsible for sizeable crop losses annually and is found in many cotton-producing regions, including the Republic of Uzbekistan and the United States. Specifically, FOV race 4 (FOV4) has been disrupting production for years. This study aimed to genetically characterize FOV4 isolates causing disease in the main cotton-producing region of Uzbekistan and compare them with FOV4 isolates from the United States. A field study conducted in the Bukhara region of the Republic of Uzbekistan in the spring of 2022 identified both FOV4 and new Fusarium isolates from Upland cotton exhibiting typical Fusarium wilt symptoms. Molecular markers were initially used to identify isolates of interest, and a phylogenetic analysis was performed using partial EF1-α sequences, followed by a comparative genomic analysis. We also report for the first time the isolation of F. solani and F. commune causing Fusarium wilt in Uzbekistan. Furthermore, we show that the FOV4 population within our sampling region of Uzbekistan may be dominated by a single biotype with an effector profile similar to that of FOV race 7. One of these effector proteins is also present in the F. commune isolate showing virulence to cotton. Whole-genome comparisons between FOV races can identify unique genetic markers for FOV4 and aid in the development of tools for breeding FOV-resistant cotton varieties.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"54-65"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transient Expression of Nicotiana tabacum Silicon-Induced Histidine-Rich Defensins in N. benthamiana Limits Necrotic Lesion Development Caused by Phytopathogenic Fungi. 在烟草根中瞬时表达烟草硅诱导的富组氨酸防御素，可限制由植物病原真菌引起的坏死性病变的发展。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2025-01-01 Epub Date: 2025-01-09 DOI: 10.1094/PHYTO-05-24-0162-R

Stephen Muhindi, Wendy Zellner, Shin-Yi Marzano, Jennifer Boldt, Scott Leisner

{"title":"Transient Expression of Nicotiana tabacum Silicon-Induced Histidine-Rich Defensins in N. benthamiana Limits Necrotic Lesion Development Caused by Phytopathogenic Fungi.","authors":"Stephen Muhindi, Wendy Zellner, Shin-Yi Marzano, Jennifer Boldt, Scott Leisner","doi":"10.1094/PHYTO-05-24-0162-R","DOIUrl":"10.1094/PHYTO-05-24-0162-R","url":null,"abstract":"Silicon (Si) supplementation permits plants to better deter infection. Supplementing hydroponically propagated Nicotiana tabacum with 1 mM potassium silicate (K2SiO3) reduced necrotic lesion development on detached leaves by both Botrytis cinerea and Sclerotinia sclerotiorum. Previously, a family of Si-induced genes was identified in N. tabacum. These genes were members of the solanaceous histidine-rich defensin (HRD) superfamily and were termed NtHRD1s (the first identified family of N. tabacum HRDs). Defensins were originally identified to participate in innate immunity. Thus, the NtHRD1s were tested for antimicrobial effects on plant pathogens. Transient expression of NtHRD1 genes within N. benthamiana leaves restricted the development of necrotic lesions caused by B. cinerea and S. sclerotiorum. Thus, the NtHRD1s may be an additional Si-responsive factor conferring beneficial effects on plants. [Formula: see text] Copyright © 2025 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"35-43"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142352513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plasmodiophora brassicae Effector PbPE23 Induces Necrotic Responses in Both Host and Nonhost Plants. 黄铜疫霉菌效应子 PbPE23 可诱导寄主植物和非寄主植物产生坏死反应。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2025-01-01 Epub Date: 2024-12-26 DOI: 10.1094/PHYTO-02-24-0064-R

Md Musharaf Hossain, Edel Pérez-López, Christopher D Todd, Yangdou Wei, Peta C Bonham-Smith

{"title":"Plasmodiophora brassicae Effector PbPE23 Induces Necrotic Responses in Both Host and Nonhost Plants.","authors":"Md Musharaf Hossain, Edel Pérez-López, Christopher D Todd, Yangdou Wei, Peta C Bonham-Smith","doi":"10.1094/PHYTO-02-24-0064-R","DOIUrl":"10.1094/PHYTO-02-24-0064-R","url":null,"abstract":"Plasmodiophora brassicae is an obligate biotroph that causes clubroot disease in cruciferous plants, including canola and Arabidopsis. In contrast to most known bacterial, oomycete, and fungal pathogens that colonize at the host apoplastic space, the protist P. brassicae establishes an intracellular colonization within various types of root cells and secretes a plethora of effector proteins to distinct cellular compartments favorable for the survival and growth of the pathogen during pathogenesis. Identification and functional characterization of P. brassicae effectors has been hampered by the limited understanding of this unique pathosystem. Here, we report a P. brassicae effector, PbPE23, containing a serine/threonine kinase domain, that induces necrosis after heterologous expression by leaf infiltration in both host and nonhost plants. Although PbPE23 is an active kinase, the kinase activity itself is not required for triggering necrosis in plants. PbPE23 shows a nucleocytoplasmic localization in Nicotiana benthamiana, and its N-terminal 25TPDPAQKQ32 sequence, resembling the contiguous hydrophilic TPAP motif and Q-rich region in many necrosis and ethylene inducing peptide 1-like proteins from plant-associated microbes, is required for the induction of necrosis. Furthermore, transcript profiling of PbPE23 reveals its high expression at the transition stages from primary to secondary infection, suggesting its potential involvement in the development of clubroot disease.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"66-76"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142293749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A New Threat to Limber Pine (Pinus flexilis) Restoration in Alberta and Beyond: First Documentation of a Cronartium ribicola Race (vcr4) Virulent to Cr4-Controlled Major Gene Resistance. 阿尔伯塔省及其他地区林缘松（Pinus flexilis）恢复面临的新威胁：Cronartium ribicola race (vcr4) 病毒对 Cr4 控制的主要抗性基因的首次记录。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2025-01-01 Epub Date: 2025-01-09 DOI: 10.1094/PHYTO-04-24-0129-R

Jun-Jun Liu, Richard A Sniezko, Sydney Houston, Genoa Alger, Jodie Krakowski, Anna W Schoettle, Robert Sissons, Arezoo Zamany, Holly Williams, Benjamin Rancourt, Angelia Kegley

{"title":"A New Threat to Limber Pine (Pinus flexilis) Restoration in Alberta and Beyond: First Documentation of a Cronartium ribicola Race (vcr4) Virulent to Cr4-Controlled Major Gene Resistance.","authors":"Jun-Jun Liu, Richard A Sniezko, Sydney Houston, Genoa Alger, Jodie Krakowski, Anna W Schoettle, Robert Sissons, Arezoo Zamany, Holly Williams, Benjamin Rancourt, Angelia Kegley","doi":"10.1094/PHYTO-04-24-0129-R","DOIUrl":"10.1094/PHYTO-04-24-0129-R","url":null,"abstract":"The coevolution of virulence reduces the effectiveness of host resistance to pathogens, posing a direct threat to forest species and their key ecosystem functions. This is a threat to limber pine (Pinus flexilis), an endangered species in Canada due to rapid decline mainly driven by white pine blister rust caused by Cronartium ribicola. We present the first report of a new, virulent race of C. ribicola (designated vcr4) that overcomes limber pine major gene (Cr4) resistance (MGR). Field surveys found that three parental trees (pf-503, pf-508, and pf-2015-0070) were cankered with white pine blister rust in Alberta, but their progenies showed MGR-related phenotypic segregation postinoculation with an avirulent race of C. ribicola (Avcr4). Genotyping of their progenies using Cr4-linked DNA markers and a genome-wide association study provided additional support that these cankered parental trees had Cr4-controlled MGR. To confirm the presence of vcr4, aeciospores were collected from the cankered pf-503 tree to inoculate resistant seedlings that had survived prior inoculation using the Avcr4 race, as well as seedlings of two U.S. seed parents, one previously confirmed with MGR (Cr4) and one without MGR, respectively. All inoculated seedlings showed clear stem symptoms, confirming that the virulent race is vcr4. These results provide insights into the evolution of C. ribicola virulence and reinforce caution on deployment of Cr4-controlled MGR. The information will be useful for designing a breeding program for durable resistance by layering both R genes with quantitative trait loci for resistance to white pine blister rust in North America.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"44-53"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142352510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Noninvasive, Presymptomatic Detection of Potato Cyst Nematode Infection in Tomato Using Chlorophyll Fluorescence Analysis. 利用叶绿素荧光分析对番茄马铃薯胞囊线虫感染进行无创、症状前检测。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2025-01-01 Epub Date: 2025-01-09 DOI: 10.1094/PHYTO-07-24-0206-R

Robbert van Himbeeck, Eline Laura Binnebösz, Deisy Amora, Michele Gottardi, Jaap-Jan Willig, Stefan Geisen, Johannes Helder

{"title":"Noninvasive, Presymptomatic Detection of Potato Cyst Nematode Infection in Tomato Using Chlorophyll Fluorescence Analysis.","authors":"Robbert van Himbeeck, Eline Laura Binnebösz, Deisy Amora, Michele Gottardi, Jaap-Jan Willig, Stefan Geisen, Johannes Helder","doi":"10.1094/PHYTO-07-24-0206-R","DOIUrl":"10.1094/PHYTO-07-24-0206-R","url":null,"abstract":"Potato cyst nematodes (PCNs) are notorious pathogens in all major potato production areas worldwide. Mainly due to the low mobility of this soil pathogen, PCN infestations are mostly observed as patches (\"foci\") that only cover a fraction of the acreage. In-field presymptomatic localization of these pathogens is valuable, as it would allow for the localized application of control measures. Although the mapping of foci is technically feasible, it is unpractical, as it would require the analysis of numerous soil samples. We investigated whether chlorophyll fluorescence (Chl-F) could be suitable as a rapid, nondestructive method for early PCN detection. To this end, the impact of four Globodera pallida densities on the Chl-F of tomato was investigated in a phenotyping greenhouse for 26 days. Furthermore, the classical plant performance indicators of biomass and root surface area were compared with Chl-F. Thermal dissipation (NPQ) and an estimate of the photosynthetic rate (ΦPSII) responded at 1 day postinoculation, and ΦPSII was most sensitive to low PCN infection levels. Chl-F parameters responded more readily to PCN infection than biomass and root surface area. The maximum quantum yield of photosystem II (Fv/Fm) and the potential activity of photosystem II (Fv/F0) initially increased at low PCN infection levels, whereas a sharp decrease was observed at higher infestation levels. Hence, our data suggest that low PCN levels promoted plant performance before becoming detrimental at higher levels. Although Chl-F allowed for early and sensitive PCN detection, it remains to be investigated whether these signals can be distinguished from those produced by other belowground stressors in the field. [Formula: see text] Copyright © 2025 The Author(s). This is an open access article distributed under the CC BY 4.0 International license.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"77-84"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142293750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High Genetic Diversity and Limited Regional Population Differentiation in Populations of Calonectria pseudoreteaudii from Eucalyptus Plantations. 桉树种植园中 Calonectria pseudoreteaudii 种群的遗传多样性较高，区域种群分化有限。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2025-01-01 Epub Date: 2025-01-17 DOI: 10.1094/PHYTO-05-24-0154-R

WenWen Li, FeiFei Liu, ShuaiFei Chen, Michael J Wingfield, Tuan A Duong

{"title":"High Genetic Diversity and Limited Regional Population Differentiation in Populations of Calonectria pseudoreteaudii from Eucalyptus Plantations.","authors":"WenWen Li, FeiFei Liu, ShuaiFei Chen, Michael J Wingfield, Tuan A Duong","doi":"10.1094/PHYTO-05-24-0154-R","DOIUrl":"10.1094/PHYTO-05-24-0154-R","url":null,"abstract":"Calonectria pseudoreteaudii causes a serious and widespread disease known as Calonectria leaf blight in Eucalyptus plantations of southern China. Little is known regarding the population biology or reproductive biology of this pathogen in the affected areas. The aims of this study were to investigate the genetic diversity, population structure, and reproductive mode of C. pseudoreteaudii from affected Eucalyptus plantations of southern China. Ten polymorphic simple sequence repeat markers were developed for the species and were used to genotype 311 isolates from eight populations. The mating types of all isolates were identified using the MAT gene primers. The results revealed a high level of genetic diversity of the pathogen in all investigated populations. Of the 90 multilocus genotypes detected, 10 were shared between at least two populations. With the exception of one population from HuiZhou, GuangDong (7HZ), the most dominant genotype was shared in the seven remaining populations. Discriminant analysis of principal components and population differentiation analyses showed that the 7HZ population was well differentiated from the others and that there was no significant differentiation between the remaining populations. Analysis of molecular variance suggested that most molecular variation was within populations (86%). Index of association analysis was consistent with a predominantly asexual life cycle for C. pseudoreteaudii in the studied regions. Although both mating types were detected in seven of the eight populations, the MAT1-1/MAT1-2 ratios in these populations deviated significantly from the 1:1 ratio expected in a randomly mating population.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"97-105"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142352512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New, Complete Circularized Genomes of Xanthomonas citri pv. mangiferaeindicae Produced from Short- and Long-Read Co-Assembly Shed Light on Strains that Emerged a Decade Ago on Mango and Cashew in Burkina Faso. 新的、完整的柠檬黄单胞菌（Xanthomonas citri pv. mangiferaeindicae）环化基因组是通过短读码和长读码联合组装产生的，它揭示了十年前在布基纳法索芒果和腰果上出现的菌株。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2025-01-01 Epub Date: 2025-01-17 DOI: 10.1094/PHYTO-08-24-0267-SC

Claudine Boyer, Pierre Lefeuvre, Cyrille Zombre, Adrien Rieux, Issa Wonni, Lionel Gagnevin, Olivier Pruvost

{"title":"New, Complete Circularized Genomes of Xanthomonas citri pv. mangiferaeindicae Produced from Short- and Long-Read Co-Assembly Shed Light on Strains that Emerged a Decade Ago on Mango and Cashew in Burkina Faso.","authors":"Claudine Boyer, Pierre Lefeuvre, Cyrille Zombre, Adrien Rieux, Issa Wonni, Lionel Gagnevin, Olivier Pruvost","doi":"10.1094/PHYTO-08-24-0267-SC","DOIUrl":"10.1094/PHYTO-08-24-0267-SC","url":null,"abstract":"We report high-quality genomes of three strains of Xanthomonas citri pv. mangiferaeindicae, the causal agent of mango bacterial canker disease, including the pathotype strain of this pathovar and two strains from Burkina Faso that emerged a decade ago. These strains hosted two to three plasmids of sizes ranging from 19 to 86 kb. Genome mining revealed the presence of several secretion systems and effectors involved in the virulence of xanthomonads with (i) a type I secretion system of the hlyDB group; (ii) xps and xcs type II secretion systems; (iii) a type III secretion system with several type III effectors, including transcription activator-like effectors; (iv) several type IV secretion systems associated with plasmid or integrative conjugative elements mobility; (v) three type V secretion system subclasses (Va, Vb, and Vc); and (vi) a single i3* type VI secretion system. The two strains isolated in Burkina Faso from mango (Mangifera indica) and cashew (Anacardium occidentale) differed by only 14 single-nucleotide polymorphisms and shared identical secretion systems and type III effector repertoires. Several transcription activator-like effectors were identified in each strain, some of which may target plant genes previously found implicated in disease development in other xanthomonad-associated pathosystems. These results support the emergence in Burkina Faso a decade ago of very closely related strains that became epidemic on mango and cashew (i.e., two distinct host genera of a same plant family). These new genomic resources will contribute to better understanding the biology and evolution of this agriculturally major crop pathogen.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"14-19"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pink-pigmented variant of Clavibacter michiganensis expands phenotypic range of tomato bacterial canker pathogen. Clavibacter michiganensis 的粉红色变种扩大了番茄细菌性腐烂病病原体的表型范围。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-12-10 DOI: 10.1094/PHYTO-07-24-0236-R

Malihe Haghverdi, S Mohsen Taghavi, Sadegh Zarei, Hamzeh Mafakheri, Hamid Abachi, Martial Briand, Geraldine Taghouti, Perrine Portier, Marie-Agnes Jacques, Ebrahim Osdaghi

{"title":"Pink-pigmented variant of Clavibacter michiganensis expands phenotypic range of tomato bacterial canker pathogen.","authors":"Malihe Haghverdi, S Mohsen Taghavi, Sadegh Zarei, Hamzeh Mafakheri, Hamid Abachi, Martial Briand, Geraldine Taghouti, Perrine Portier, Marie-Agnes Jacques, Ebrahim Osdaghi","doi":"10.1094/PHYTO-07-24-0236-R","DOIUrl":"https://doi.org/10.1094/PHYTO-07-24-0236-R","url":null,"abstract":"Bacterial canker of tomato caused by the Gram-positive corynebacterial species Clavibacter michiganensis is one of the most destructive seed-borne diseases in both open air and greenhouse tomatoes. The pathogen is a regulated agent in all tomato-producing countries as translocation of infected tomato materials transports the bacterium into new areas. Clavibacter michiganensis is generally known to have yellow-pigmented colonies on culture media, which is a key differentiative phenotypic feature in standard diagnostic guidelines. During 2020 and 2021, pink-pigmented corynebacterial strains were isolated from tomato seeds (cv. Sun 6189F1) and plants showing severe canker symptoms in Southern Iran. The six pink-pigmented strains were pathogenic on tomato and pepper seedlings under greenhouse conditions, and gave positive results with C. michiganensis-specific primers pairs described in the literature. Phylogenomics and DNA similarity calculations showed that the pink-pigmented strains were highly similar to the authentic yellow-pigmented members of the pathogen. Thus, they were identified as a new phenotypic variant of tomato bacterial canker pathogen. Whole genome screenings accomplished with PCR-based assays showed that the pink strains contain all pathogenicity determinant genes described in C. michiganensis. Further, orthologous gene clusters in the pink-pigmented strains were more similar to the pathogenic members of C. michiganensis than to those of non-pathogenic tomato-associated Clavibacter species. Results obtained in this study demonstrate the emergence of a new pink-pigmented variant of C. michiganensis and highlight the importance of colony pigmentation/morphology in culture-based detection of the bacterium. The need for updating diagnostic guidelines on the colony variants of the pathogen is further discussed.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142802074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0