Eva Garcia, Alexandra K Kahn, Cristiana Rodrigues, Alexandra Camelo, Christophe Espirito Santo, Helvecio D Coletta-Filho, Rodrigo P P Almeida, Joana Costa
{"title":"苛养木杆菌亚种的分离、系统发育推断及早期分化。葡萄牙Cova da Beira地区的fastidiosa。","authors":"Eva Garcia, Alexandra K Kahn, Cristiana Rodrigues, Alexandra Camelo, Christophe Espirito Santo, Helvecio D Coletta-Filho, Rodrigo P P Almeida, Joana Costa","doi":"10.1094/PHYTO-01-25-0009-R","DOIUrl":null,"url":null,"abstract":"<p><p>The introduction of <i>Xylella fastidiosa</i>, a plant pathogen with a broad host range, poses a significant threat to agriculture and natural ecosystems. We analysed six <i>X. fastidiosa</i> subsp. <i>fastidiosa</i> strains obtained from the Beira Interior region of Portugal, an area known for fruit production. Whole-genome sequencing and phylogenetic analyses were performed to genetically characterize these strains and determine their origin. The results suggest that these outbreak-demarcated areas originated from a single introduction event traced back to California, USA. All six strains belong to subspecies <i>fastidiosa</i>, sequence type ST1, which has been reported to infect a variety of economically important crops, including cherry, plum, almond, and grapevine. A molecular clock analysis estimated that the introduction occurred between 2010 and 2020 (95% HPD 1999 - 2022), with the strains forming a clade that diverged circa 2020. The findings underscore the importance of continued surveillance in both agricultural and unmanaged ecosystems, as the presence and potential impact of <i>X. fastidiosa</i> on Portuguese landscapes remain largely unknown.</p>","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":""},"PeriodicalIF":3.1000,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Isolation, Phylogenetic Inferences, and Early Diversification of <i>Xylella fastidiosa</i> subsp. <i>fastidiosa</i> in Cova da Beira Region, Portugal.\",\"authors\":\"Eva Garcia, Alexandra K Kahn, Cristiana Rodrigues, Alexandra Camelo, Christophe Espirito Santo, Helvecio D Coletta-Filho, Rodrigo P P Almeida, Joana Costa\",\"doi\":\"10.1094/PHYTO-01-25-0009-R\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The introduction of <i>Xylella fastidiosa</i>, a plant pathogen with a broad host range, poses a significant threat to agriculture and natural ecosystems. We analysed six <i>X. fastidiosa</i> subsp. <i>fastidiosa</i> strains obtained from the Beira Interior region of Portugal, an area known for fruit production. Whole-genome sequencing and phylogenetic analyses were performed to genetically characterize these strains and determine their origin. The results suggest that these outbreak-demarcated areas originated from a single introduction event traced back to California, USA. All six strains belong to subspecies <i>fastidiosa</i>, sequence type ST1, which has been reported to infect a variety of economically important crops, including cherry, plum, almond, and grapevine. A molecular clock analysis estimated that the introduction occurred between 2010 and 2020 (95% HPD 1999 - 2022), with the strains forming a clade that diverged circa 2020. The findings underscore the importance of continued surveillance in both agricultural and unmanaged ecosystems, as the presence and potential impact of <i>X. fastidiosa</i> on Portuguese landscapes remain largely unknown.</p>\",\"PeriodicalId\":20410,\"journal\":{\"name\":\"Phytopathology\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":3.1000,\"publicationDate\":\"2025-05-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Phytopathology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1094/PHYTO-01-25-0009-R\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Phytopathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1094/PHYTO-01-25-0009-R","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Isolation, Phylogenetic Inferences, and Early Diversification of Xylella fastidiosa subsp. fastidiosa in Cova da Beira Region, Portugal.
The introduction of Xylella fastidiosa, a plant pathogen with a broad host range, poses a significant threat to agriculture and natural ecosystems. We analysed six X. fastidiosa subsp. fastidiosa strains obtained from the Beira Interior region of Portugal, an area known for fruit production. Whole-genome sequencing and phylogenetic analyses were performed to genetically characterize these strains and determine their origin. The results suggest that these outbreak-demarcated areas originated from a single introduction event traced back to California, USA. All six strains belong to subspecies fastidiosa, sequence type ST1, which has been reported to infect a variety of economically important crops, including cherry, plum, almond, and grapevine. A molecular clock analysis estimated that the introduction occurred between 2010 and 2020 (95% HPD 1999 - 2022), with the strains forming a clade that diverged circa 2020. The findings underscore the importance of continued surveillance in both agricultural and unmanaged ecosystems, as the presence and potential impact of X. fastidiosa on Portuguese landscapes remain largely unknown.
期刊介绍:
Phytopathology publishes articles on fundamental research that advances understanding of the nature of plant diseases, the agents that cause them, their spread, the losses they cause, and measures that can be used to control them. Phytopathology considers manuscripts covering all aspects of plant diseases including bacteriology, host-parasite biochemistry and cell biology, biological control, disease control and pest management, description of new pathogen species description of new pathogen species, ecology and population biology, epidemiology, disease etiology, host genetics and resistance, mycology, nematology, plant stress and abiotic disorders, postharvest pathology and mycotoxins, and virology. Papers dealing mainly with taxonomy, such as descriptions of new plant pathogen taxa are acceptable if they include plant disease research results such as pathogenicity, host range, etc. Taxonomic papers that focus on classification, identification, and nomenclature below the subspecies level may also be submitted to Phytopathology.