{"title":"外源BR通过改善水稻活性氧稳态和光合作用增强宿主对茄枯核菌的抗性。","authors":"Kejin Chu, Jiejing Dong, Hongyu Li, Changjiang Zhao","doi":"10.1094/PHYTO-10-24-0322-R","DOIUrl":null,"url":null,"abstract":"<p><p>Brassinosteroid (BR) is essential in regulating plant growth and development and response to stress. However, there are few reports on the mechanism of BR regulating rice resistance to necrotrophic fungus. In this study, rice seedlings were pretreated with BR hormone and its synthetic inhibitor Brassinazole (BRZ) and inoculated with <i>Rhizoctonia solani</i> to analyze the reactive oxygen species (ROS), photosynthetic indices, and expression of phytohormones signal components and defense-related genes in rice leaves during pathogen infection. BR treatment significantly decreased the lesion area, significantly increased the activity of antioxidant enzymes and the content of antioxidant substances, and significantly decreased the contents of O<sub>2</sub>-, H<sub>2</sub>O<sub>2</sub>, and MDA. At the same time, BR treatment enhanced photosynthetic pigment content and Fv/Fm value of rice seedlings. In addition, BR treatment can cause high expression of endogenous BR synthesis and decomposition genes and signal transduction genes, cooperate with SA, and antagonize JA signal gene expression. The structural equation analysis of tested indices uncovered firstly that high BR level stimulated the BR signal transduction pathway to regulate photosynthesis and ROS homeostasis through ROS signal, thereby enhancing the resistance of rice seedlings to <i>R. solani</i>. This study provides theoretical guidance for the application of BR analogues chemical regulators.</p>","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Exogenous BR Enhances Host Resistance to <i>Rhizoctonia solani</i> Through Reactive Oxygen Species Homeostasis and Photosynthesis Improvement in <i>Oryza sativa</i>.\",\"authors\":\"Kejin Chu, Jiejing Dong, Hongyu Li, Changjiang Zhao\",\"doi\":\"10.1094/PHYTO-10-24-0322-R\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Brassinosteroid (BR) is essential in regulating plant growth and development and response to stress. However, there are few reports on the mechanism of BR regulating rice resistance to necrotrophic fungus. In this study, rice seedlings were pretreated with BR hormone and its synthetic inhibitor Brassinazole (BRZ) and inoculated with <i>Rhizoctonia solani</i> to analyze the reactive oxygen species (ROS), photosynthetic indices, and expression of phytohormones signal components and defense-related genes in rice leaves during pathogen infection. BR treatment significantly decreased the lesion area, significantly increased the activity of antioxidant enzymes and the content of antioxidant substances, and significantly decreased the contents of O<sub>2</sub>-, H<sub>2</sub>O<sub>2</sub>, and MDA. At the same time, BR treatment enhanced photosynthetic pigment content and Fv/Fm value of rice seedlings. In addition, BR treatment can cause high expression of endogenous BR synthesis and decomposition genes and signal transduction genes, cooperate with SA, and antagonize JA signal gene expression. The structural equation analysis of tested indices uncovered firstly that high BR level stimulated the BR signal transduction pathway to regulate photosynthesis and ROS homeostasis through ROS signal, thereby enhancing the resistance of rice seedlings to <i>R. solani</i>. This study provides theoretical guidance for the application of BR analogues chemical regulators.</p>\",\"PeriodicalId\":20410,\"journal\":{\"name\":\"Phytopathology\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2025-02-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Phytopathology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1094/PHYTO-10-24-0322-R\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Phytopathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1094/PHYTO-10-24-0322-R","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Exogenous BR Enhances Host Resistance to Rhizoctonia solani Through Reactive Oxygen Species Homeostasis and Photosynthesis Improvement in Oryza sativa.
Brassinosteroid (BR) is essential in regulating plant growth and development and response to stress. However, there are few reports on the mechanism of BR regulating rice resistance to necrotrophic fungus. In this study, rice seedlings were pretreated with BR hormone and its synthetic inhibitor Brassinazole (BRZ) and inoculated with Rhizoctonia solani to analyze the reactive oxygen species (ROS), photosynthetic indices, and expression of phytohormones signal components and defense-related genes in rice leaves during pathogen infection. BR treatment significantly decreased the lesion area, significantly increased the activity of antioxidant enzymes and the content of antioxidant substances, and significantly decreased the contents of O2-, H2O2, and MDA. At the same time, BR treatment enhanced photosynthetic pigment content and Fv/Fm value of rice seedlings. In addition, BR treatment can cause high expression of endogenous BR synthesis and decomposition genes and signal transduction genes, cooperate with SA, and antagonize JA signal gene expression. The structural equation analysis of tested indices uncovered firstly that high BR level stimulated the BR signal transduction pathway to regulate photosynthesis and ROS homeostasis through ROS signal, thereby enhancing the resistance of rice seedlings to R. solani. This study provides theoretical guidance for the application of BR analogues chemical regulators.
期刊介绍：
Phytopathology publishes articles on fundamental research that advances understanding of the nature of plant diseases, the agents that cause them, their spread, the losses they cause, and measures that can be used to control them. Phytopathology considers manuscripts covering all aspects of plant diseases including bacteriology, host-parasite biochemistry and cell biology, biological control, disease control and pest management, description of new pathogen species description of new pathogen species, ecology and population biology, epidemiology, disease etiology, host genetics and resistance, mycology, nematology, plant stress and abiotic disorders, postharvest pathology and mycotoxins, and virology. Papers dealing mainly with taxonomy, such as descriptions of new plant pathogen taxa are acceptable if they include plant disease research results such as pathogenicity, host range, etc. Taxonomic papers that focus on classification, identification, and nomenclature below the subspecies level may also be submitted to Phytopathology.
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