Phytopathology最新文献_第8页

Effect of Initial Inoculum on the Temporal and Spatial Dynamics of Wheat Blast Under Field Conditions in Bolivia. 玻利维亚田间条件下初始接种量对小麦稻瘟病时空动态的影响。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-10-01 Epub Date: 2024-10-03 DOI: 10.1094/PHYTO-12-23-0491-R

Carlos C Góngora-Canul, Alexandria Volkening, Jorge Cuéllar, Lidia Calderón, Mariela Fernández-Campos, Da Young Lee, Jorge Salgado, Andres Cruz-Sancan, C D Cruz

{"title":"Effect of Initial Inoculum on the Temporal and Spatial Dynamics of Wheat Blast Under Field Conditions in Bolivia.","authors":"Carlos C Góngora-Canul, Alexandria Volkening, Jorge Cuéllar, Lidia Calderón, Mariela Fernández-Campos, Da Young Lee, Jorge Salgado, Andres Cruz-Sancan, C D Cruz","doi":"10.1094/PHYTO-12-23-0491-R","DOIUrl":"10.1094/PHYTO-12-23-0491-R","url":null,"abstract":"Epidemiological studies to better understand wheat blast (WB) spatial and temporal patterns were conducted in three field environments in Bolivia between 2019 and 2020. The temporal dynamics of wheat leaf blast (WLB) and spike blast (WSB) were best described by the logistic model compared with the Gompertz and exponential models. The nonlinear logistic infection rates were higher under defined inoculation in experiments two and three than under undefined inoculation in experiment one, and they were also higher for WSB than for WLB. The onset of WLB began with a spatial clustering pattern according to autocorrelation analysis and Moran's index values, with higher severity and earlier onset for defined than for undefined inoculation until the last sampling time. The WSB onset did not start with a spatial clustering pattern; instead, it was detected later until the last sampling date across experiments, with higher severity and earlier onset for defined than for undefined inoculation. Maximum severity (Kmax) was 1.0 for WSB and less than 1.0 for WLB. Aggregation of WLB and WSB was higher for defined than for undefined inoculation. The directionality of hotspot development was similar for both WLB and WSB, mainly occurring concentrically for defined inoculation. Our results show no evidence of synchronized development but suggest a temporal and spatial progression of disease symptoms on wheat leaves and spikes. Thus, we recommend that monitoring and management of WB should be considered during early growth stages of wheat planted in areas of high risk.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"2273-2286"},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141559505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pseudomonas syringae pv. actinidiae Unique Effector HopZ5 Interacts with GF14C to Trigger Plant Immunity. Pseudomonas syringae pv. actinidiae 独特的效应因子 HopZ5 与 GF14C 相互作用，触发植物免疫。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-10-01 Epub Date: 2024-10-04 DOI: 10.1094/PHYTO-09-23-0330-R

Mingxia Zhou, Jinglong Zhang, Zhibo Zhao, Wei Liu, Zhiran Wu, Lili Huang

{"title":"Pseudomonas syringae pv. actinidiae Unique Effector HopZ5 Interacts with GF14C to Trigger Plant Immunity.","authors":"Mingxia Zhou, Jinglong Zhang, Zhibo Zhao, Wei Liu, Zhiran Wu, Lili Huang","doi":"10.1094/PHYTO-09-23-0330-R","DOIUrl":"10.1094/PHYTO-09-23-0330-R","url":null,"abstract":"The bacterial canker of kiwifruit caused by Pseudomonas syringae pv. actinidiae (Psa) is the most devastating disease threatening the global kiwifruit production. This pathogen delivers multiple effector proteins into plant cells to resist plant immune responses and facilitate their survival. Here, we focused on the unique effector HopZ5 in Psa, which previously has been reported to have virulence functions. In this study, our results showed that HopZ5 could cause macroscopic cell death and trigger a serious immune response by agroinfiltration in Nicotiana benthamiana, along with upregulated expression of immunity-related genes and significant accumulation of reactive oxygen species and callose. Subsequently, we confirmed that HopZ5 interacted with the phosphoserine-binding protein GF14C in both the nonhost plant N. benthamiana (NbGF14C) and the host plant kiwifruit (AcGF14C), and silencing of NbGF14C compromised HopZ5-mediated cell death, suggesting that GF14C plays a crucial role in the detection of HopZ5. Further studies showed that overexpression of NbGF14C both markedly reduced the infection of Sclerotinia sclerotiorum and Phytophthora capsica in N. benthamiana, and overexpression of AcGF14C significantly enhanced the resistance of kiwifruit against Psa, indicating that GF14C positively regulates plant immunity. Collectively, our results revealed that the virulence effector HopZ5 could be recognized by plants and interact with GF14C to activate plant immunity.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"2322-2330"},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141894112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effectiveness and Genetic Control of Trichoderma spp. as a Biological Control of Wheat Powdery Mildew Disease. 毛霉菌作为小麦白粉病生物防治的有效性和遗传控制。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-10-01 Epub Date: 2024-10-16 DOI: 10.1094/PHYTO-05-24-0157-R

Amira M I Mourad, Andreas Börner, Samar M Esmail

{"title":"Effectiveness and Genetic Control of Trichoderma spp. as a Biological Control of Wheat Powdery Mildew Disease.","authors":"Amira M I Mourad, Andreas Börner, Samar M Esmail","doi":"10.1094/PHYTO-05-24-0157-R","DOIUrl":"10.1094/PHYTO-05-24-0157-R","url":null,"abstract":"Wheat powdery mildew (WPM) is one of the most devasting diseases that affects wheat yield worldwide. Few efforts have been made to control such a serious disease. An effective way to control WPM is urgently needed. Biological control is an effective way to control plant diseases worldwide. In this study, the efficiency of three different Trichoderma spp. in controlling WPM at the seedling growth stage was tested using 35 highly diverse wheat genotypes. Highly significant differences were found in WPM resistance among the four treatments, confirming the efficiency of Trichoderma in controlling WPM. Of the three species, T. asperellum T34 (T34) was the most effective species in controlling WPM, as it reduced the symptoms by 50.56%. A set of 196 wheat genotypes was used to identify the genetic control of the WPM resistance induced by T34. A total of 39, 27, and 18 gene models were identified to contain the significant markers under Pm, T34, and the improvement in powdery mildew resistance due to T34 (T34_improvement) conditions. Furthermore, no gene model was common between T34 and Pm, suggesting the presence of completely different genetic systems controlling the resistance under T34 and Pm. The functional annotation and biological process pathways of the detected gene models confirm their association with the normal and induced resistance. This study, for the first time, confirms the efficiency of T34 in controlling WPM and provides a deep understanding of the genetic control of induced and normal resistance to WPM.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"2221-2234"},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141545197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combining Single-Gene-Resistant and Pyramided Cultivars of Perennial Crops in Agricultural Landscapes Compromises Pyramiding Benefits in Most Production Situations. 在农业景观中将多年生作物的单基因抗性品种与金字塔型栽培品种结合起来，在大多数生产情况下都会损害金字塔型栽培的效益。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-10-01 Epub Date: 2024-10-15 DOI: 10.1094/PHYTO-02-24-0075-R

Marta Zaffaroni, Julien Papaïx, Abebayehu G Geffersa, Jean-François Rey, Loup Rimbaud, Frédéric Fabre

{"title":"Combining Single-Gene-Resistant and Pyramided Cultivars of Perennial Crops in Agricultural Landscapes Compromises Pyramiding Benefits in Most Production Situations.","authors":"Marta Zaffaroni, Julien Papaïx, Abebayehu G Geffersa, Jean-François Rey, Loup Rimbaud, Frédéric Fabre","doi":"10.1094/PHYTO-02-24-0075-R","DOIUrl":"10.1094/PHYTO-02-24-0075-R","url":null,"abstract":"Although resistant cultivars are valuable in safeguarding crops against diseases, they can be rapidly overcome by pathogens. Numerous strategies have been proposed to delay pathogen adaptation (evolutionary control) while still ensuring effective protection (epidemiological control). For perennial crops, multiple resistance genes can be deployed (i) in the same cultivar (pyramiding strategy); in single-gene-resistant cultivars grown (ii) in the same field (mixture strategy) or (iii) in different fields (mosaic strategy); or (iv) in hybrid strategies that combine the three previous options. In addition, the spatial scale at which resistant cultivars are deployed can affect the plant-pathogen interaction: Small fields are thought to reduce pest density and disease transmission. Here, we used the spatially explicit stochastic model landsepi to compare the evolutionary and epidemiological control across spatial scales and deployment strategies relying on two major resistance genes. Our results, broadly focused on resistance to downy mildew of grapevine, show that the evolutionary control provided by the pyramiding strategy is at risk when single-gene-resistant cultivars are concurrently planted in the landscape (hybrid strategies), especially at low mutation probability. Moreover, the effectiveness of pyramiding compared with hybrid strategies is influenced by whether the adapted pathogen pays a fitness cost across all hosts or only for unnecessary virulence, particularly when the fitness cost is high rather than intermediate. Finally, field size did not affect model outputs for a wide range of mutation probabilities and associated fitness costs. The socioeconomic policies favoring the adoption of optimal resistant management strategies are discussed.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"2310-2321"},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141617063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whole-Genome Resequencing Reveals Significant Genetic Differentiation Between Exserohilum turcicum Populations from Maize and Sorghum and Candidate Effector Genes Related to Host Specificity. 全基因组重测序揭示了来自玉米和高粱的 Exserohilum turcicum 群体之间的显著遗传分化，以及与宿主特异性相关的候选效应基因。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-10-01 Epub Date: 2024-10-07 DOI: 10.1094/PHYTO-05-24-0172-R

Linkai Cui, Cong Wang, Mengqi Li, Yufeng Fang, Yanhong Hu

{"title":"Whole-Genome Resequencing Reveals Significant Genetic Differentiation Between Exserohilum turcicum Populations from Maize and Sorghum and Candidate Effector Genes Related to Host Specificity.","authors":"Linkai Cui, Cong Wang, Mengqi Li, Yufeng Fang, Yanhong Hu","doi":"10.1094/PHYTO-05-24-0172-R","DOIUrl":"10.1094/PHYTO-05-24-0172-R","url":null,"abstract":"Exserohilum turcicum is a devastating fungal pathogen that infects both maize and sorghum, leading to severe leaf diseases of the two crops. According to host specificity, pathogenic isolates of E. turcicum are divided into two formae speciales, namely E. turcicum f. sp. zeae and E. turcicum f. sp. sorghi. To date, the molecular mechanism underlying the host specificity of E. turcicum is marginally known. In this study, the whole genomes of 60 E. turcicum isolates collected from both maize and sorghum were resequenced, which enabled identification of 233,022 single-nucleotide polymorphisms (SNPs) in total. Phylogenetic analysis indicated that all isolates are clustered into four genetic groups that have a close relationship with host source. This observation is validated by the result of principal component analysis. Analysis of population structure revealed that there is obvious genetic differentiation between two populations from maize and sorghum. Further analysis showed that 5,431 SNPs, including 612 nonsynonymous SNPs, are completely co-segregated with the host source. These nonsynonymous SNPs are located in 539 genes, among which 18 genes are predicted to encode secretory proteins, including six putative effector genes named SIX13-like, Ecp6, GH12, GH28-1, GH28-2, and CHP1. Sequence polymorphism analysis revealed various numbers of SNPs in the coding regions of these genes. These findings provide new insights into the molecular basis of host specificity in E. turcicum.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"2351-2359"},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141760573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rhodopseudomonas palustris Atp2 Protein Exerts Antifungal Effects by Targeting the Ribosomal Protein MoRpl12 in Magnaporthe oryzae. 黄绿假单胞菌 Atp2 蛋白通过靶向 Magnaporthe oryzae 的核糖体蛋白 MoRpl12 发挥抗真菌作用

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-10-01 DOI: 10.1094/PHYTO-05-24-0169-R

Chunyan Chen, Xiyang Wu, Qiang Huang, Yingfei Qin, Chenggang Li, Xin Zhang, Pei Wang, Xinqiu Tan, Yong Liu, Yue Chen, Deyong Zhang

{"title":"Rhodopseudomonas palustris Atp2 Protein Exerts Antifungal Effects by Targeting the Ribosomal Protein MoRpl12 in Magnaporthe oryzae.","authors":"Chunyan Chen, Xiyang Wu, Qiang Huang, Yingfei Qin, Chenggang Li, Xin Zhang, Pei Wang, Xinqiu Tan, Yong Liu, Yue Chen, Deyong Zhang","doi":"10.1094/PHYTO-05-24-0169-R","DOIUrl":"10.1094/PHYTO-05-24-0169-R","url":null,"abstract":"Rice blast is one of the most hazardous diseases affecting rice production. Previously, we discovered that the Atp2 protein of Rhodopseudomonas palustris could significantly inhibit the appressorium formation and pathogenicity of Magnaporthe oryzae. However, the molecular mechanism of this fungus has remained unknown. This study revealed that Atp2 can enter the cell and interact with the ribosomal protein MoRpl12 of M. oryzae, directly affecting the expression of the MoRpl12 protein. Silencing the MoRPL12 gene can affect cell wall integrity, growth, conidiogenesis, and fungal pathogenicity. The quantitative reverse transcription PCR results showed significant changes in the expression of conidiation-related genes in the MoRPL12 gene-silenced mutants or in the Atp2 protein-treated plants. We further found that Atp2 treatment can influence the expression of ribosomal-related genes, such as RPL, in M. oryzae. Our study revealed a novel antifungal mechanism by which the Atp2 protein binds to the ribosomal protein MoRpl12 and inhibits the pathogenicity of rice blast fungus, providing a new potential target for rice blast prevention and control.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"2235-2243"},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142366278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Validation of Fungicide Spray Strategies and Selection for Fenhexamid Resistance in Botrytis cinerea on Greenhouse-Grown Grapevines. 在温室栽培的葡萄藤上验证喷洒杀真菌剂的策略，并筛选出对芬克菌胺具有抗性的葡萄灰霉病菌。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-10-01 Epub Date: 2024-09-13 DOI: 10.1094/PHYTO-11-23-0443-R

Stephen C Boushell, Mengjun Hu

引用次数: 0

Effects of Different Light Conditions on Anatomical and Histological Features of Galls in Bacterial Gall Disease of Cerasus × yedoensis. 不同光照条件对 Cerasus × yedoensis 细菌性瘿病虫瘿解剖学和组织学特征的影响。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-10-01 Epub Date: 2024-10-14 DOI: 10.1094/PHYTO-06-22-0221-R

Takefumi Ikeda, Misaki Okuda, Makoto Ishihara, Yasuo Kon-No

{"title":"Effects of Different Light Conditions on Anatomical and Histological Features of Galls in Bacterial Gall Disease of Cerasus × yedoensis.","authors":"Takefumi Ikeda, Misaki Okuda, Makoto Ishihara, Yasuo Kon-No","doi":"10.1094/PHYTO-06-22-0221-R","DOIUrl":"10.1094/PHYTO-06-22-0221-R","url":null,"abstract":"Cerasus × yedoensis (cherry 'Somei-yoshino' Fujino) is affected by bacterial gall disease caused by Pseudomonas syringae pv. cerasicola (PSC). C. × yedoensis is often infected with PSC under weak light intensity, which indicates that susceptibility of C. × yedoensis to PSC is affected by light. To evaluate the effects of white light intensity and different light qualities, white or blue, on bacterial gall disease development, we quantitatively assessed the anatomical and histological features of bacterial-inoculated sites on branches of 2-year-old potted C. × yedoensis seedlings grown under different light intensities and qualities. The stronger the white light intensity, the less severe the gall symptoms. Gall formation was suppressed more by blue than white light of the same intensity. The validity of a simple gall index for assessing gall development with the naked eye, via quantitative evaluation of gall shape by measuring gall height, width, and volume, showed that the gall index could be used as a practical method for on-site assessments of gall development. The ratio of degeneration area in the gall remained constant, suggesting the presence of some regulatory mechanism preventing PSC from affecting the entire gall within the plant. Microscopy showed that the gall tissue is composed primarily of callus cells and has voids containing gummy material that is exuded from cracks in the gall, and the periderm develops at the gall foot but not at the gall apex, so the cells at the gall apex were necrotic or collapsed.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"2196-2206"},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141793095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Host-Driven Selection, Revealed by Comparative Analysis of Xanthomonas Type III Secretion Effectoromes, Unveils Novel Recognized Effectors. 通过比较分析黄单胞菌 III 型分泌效应器基因组发现的宿主驱动选择揭示了新型识别效应器。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-10-01 Epub Date: 2024-10-16 DOI: 10.1094/PHYTO-04-24-0147-R

Yao Xiao, Shatrupa Ray, Saul Burdman, Doron Teper

{"title":"Host-Driven Selection, Revealed by Comparative Analysis of Xanthomonas Type III Secretion Effectoromes, Unveils Novel Recognized Effectors.","authors":"Yao Xiao, Shatrupa Ray, Saul Burdman, Doron Teper","doi":"10.1094/PHYTO-04-24-0147-R","DOIUrl":"10.1094/PHYTO-04-24-0147-R","url":null,"abstract":"Xanthomonas species are specialized plant pathogens, often exhibiting a narrow host range. They rely on the translocation of effector proteins through the type III secretion system to colonize their respective hosts. The effector arsenal varies among Xanthomonas spp., typically displaying species-specific compositions. This species-specific effector composition, collectively termed the effectorome, is thought to influence host specialization. We determined the plant host-derived effectoromes of more than 300 deposited genomes of Xanthomonas species associated with either Solanaceae or Brassicaceae hosts. Comparative analyses revealed clear species-specific effectorome signatures. However, Solanaceae or Brassicaceae host-associated effectorome signatures were not detected. Nevertheless, host biases in the presence or absence of specific effector classes were observed. To assess whether host-associated effector absence results from selective pressures, we introduced effectors unique to Solanaceae pathogens to X. campestris pv. campestris and effectors unique to Brassicaceae pathogens to X. euvesicatoria pv. euvesicatoria (Xeue) and evaluated if these introductions hindered virulence on their respective hosts. Introducing the effector XopI into X. campestris pv. campestris reduced virulence on white cabbage leaves without affecting localized or systemic colonization. Introducing the XopAC or XopJ5 effectors into Xeue reduced virulence and colonization on tomato but not on pepper. Additionally, XopAC and XopJ5 induced a hypersensitive response on tomato leaves when delivered by Xeue or through Agrobacterium-mediated transient expression, confirming recognition in tomato. This study demonstrates the role of host-derived selection in establishing species-specific effectoromes, identifying XopAC and XopJ5 as recognized effectors in tomato.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"2207-2220"},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141971650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional Characterization of the Histone Acetyltransferase FcElp3 in Lotus Rhizome Rot-Causing Fungus Fusarium commune. 荷花根茎腐烂病致病真菌 Fusarium commune 中组蛋白乙酰转移酶 FcElp3 的功能特征。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-10-01 Epub Date: 2024-10-04 DOI: 10.1094/PHYTO-01-24-0017-R

Lifang Ye, Weigang Kuang, Lianhu Zhang, Yachun Lin, Yifan Zhang, Xiaotang Sun, Ruqiang Cui

{"title":"Functional Characterization of the Histone Acetyltransferase FcElp3 in Lotus Rhizome Rot-Causing Fungus Fusarium commune.","authors":"Lifang Ye, Weigang Kuang, Lianhu Zhang, Yachun Lin, Yifan Zhang, Xiaotang Sun, Ruqiang Cui","doi":"10.1094/PHYTO-01-24-0017-R","DOIUrl":"10.1094/PHYTO-01-24-0017-R","url":null,"abstract":"Fusarium commune is the main pathogen of lotus rhizome rot, which causes the wilt of many plants. Histone acetyltransferase plays a critical part in the growth and virulence of fungi. In the present study, we identified an FcElp3 in F. commune homologous to histone acetyltransferase Elp3. We further constructed a mutant strain of F. commune to determine the function of FcElp3 in fungal growth and pathogenicity. The results showed that the deletion of FcElp3 resulted in reduced mycelial growth and sporulation. Compared with the wild type, the ΔFcElp3 strain showed more tolerance to osmotic stress and cell wall stress responses but was highly sensitive to oxidative stress. The subcellular localization results indicated that FcElp3 was distributed in both the cytoplasm and nucleus. Western blotting showed that FcElp3 was important for acetylation of H3K14 and H4K8. RNA sequencing analysis showed significant transcriptional changes in the ΔFcElp3 mutant, with 3,098 genes upregulated and 5,770 genes downregulated. Peroxisome was the most significantly enriched metabolic pathway for downregulated genes. This led to a significant decrease in the expression of the core transcription factor Fcap1 involved in the oxidative stress response. Pathogenicity tests revealed that the ΔFcElp3 mutant's pathogenicity on lotus was significantly decreased. Together, these findings clearly demonstrated that FcElp3 was involved in fungal growth, development, stress response, and pathogenicity via the direct regulation of multiple target genes.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"2300-2309"},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141617064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0