Plant Signaling & Behavior最新文献

{"title":"The clonal growth in Aconitum carmichaelii Debx","authors":"Jing Gao, Ran Liu, Min Luo, Guangzhi Wang","doi":"10.1080/15592324.2022.2083818","DOIUrl":"https://doi.org/10.1080/15592324.2022.2083818","url":null,"abstract":"ABSTRACT Aconitum carmichaelii Debx. is used as traditional herbal medicine in China, Japan, and other Asian countries. A. carmichaelii has two modes for reproduction: sexual reproduction with seed and vegetative reproduction with vegetative propagules. The vegetative propagules are belowground and invisible. To date, only a handful of studies for the clonal growth are available. In this study, we investigated the clonal growth by anatomical and morphological changes. Results revealed that the axillary bud appeared on the rhizome. Furthermore, the axillary meristem in the axillary bud differentiated a bud upwards and an adventitious root (AR) downwards. The AR expanded to a tuberous root in order to provide the bud nutrients for the new plant. The AR branched LRs. In addition, some lateral roots (LRs) on the AR also swelled. Both the AR and LR were found to follow a similar pattern of development. However, high lignification in the stele region of LRs inhibited further expansion. AR development was attributed to activities of the cambium and meristem cell, starch accumulation, stele lignification, and a polyarch stele. Our study not only provides a better understanding of clonal growth but also provides clues to explore the regulatory mechanisms underlying AR development in A. carmichaelii.","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":"15 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2022-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84393045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Behavior and possible function of Arabidopsis BES1/BZR1 homolog 2 in brassinosteroid signaling","authors":"Yui Otani, Mika Kawanishi, Miyu Kamimura, A. Sasaki, Yasushi Nakamura, Takako Nakamura, S. Okamoto","doi":"10.1080/15592324.2022.2084277","DOIUrl":"https://doi.org/10.1080/15592324.2022.2084277","url":null,"abstract":"ABSTRACT Two key transcription factors (TFs) in brassinosteroid (BR) signaling BRASSINOSTEROID INSENSITIVE 1-EMS-SUPPRESSOR 1 (BES1) and BRASSINAZOLE RESISTANT 1 (BZR1), belong to a small family with four BES1/BZR1 homologs (BEH1–4). To date, in contrast to the wealth of knowledge regarding BES1 and BZR1, little is known about BEH1–4. Here, we show that BEH2 was expressed preferentially in the roots and leaf margins including serrations, which was quite different from another member BEH4, and that BRs downregulated BEH2 through a module containing GSK3-like kinases and BES1/BZR1 TFs, among which BES1, rather than BZR1, contributed to this process. In addition, BEH2 consistently existed in the nucleus, suggesting that its subcellular localization is not under BR-dependent nuclear-cytoplasmic shuttling control. Furthermore, gene ontology analysis on RNA-seq data indicated that BEH2 may be implicated in stress response and photosynthesis. These findings might assist in the future elucidation of the molecular mechanisms underlying BR signaling.","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":"7 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74654208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mobility of the syntaxin PEN1 in Arabidopsis reflects functional specialization of the conserved SYP12 clade","authors":"Mengqi Liu, Hector M Rubiato, M. E. Nielsen","doi":"10.1080/15592324.2022.2084278","DOIUrl":"https://doi.org/10.1080/15592324.2022.2084278","url":null,"abstract":"ABSTRACT Plant innate immunity toward cell-wall penetrating filamentous pathogens relies on the conserved SYP12 clade of secretory syntaxins. In Arabidopsis, the two closely related SYP12 clade members, PEN1 and SYP122, play an overlapping role in this general immunity, which can be complemented by two SYP12 clade members from Marchantia (MpSYP12A and MpSYP12B). However, in addition to the conserved SYP12 clade function, PEN1 alone mediates pre-invasive immunity toward powdery mildew fungi, which likely reflects a specialization of its functionality. Here, we show that the PEN1-specific specialization in immunity correlates with a continuous BFA-sensitive recycling and the ability to accumulate strongly at the growing cell plate. This contrasts with the behavior of SYP122, MpSYP12A, and MpSYP12B, all being more stable at the plasma membrane. We suggest that the highly mobile SYP12 specialization observed for PEN1 is required for a fast pre-invasive immune response to resist attack from powdery mildew fungi.","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":"142 1 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2022-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91110687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification and expression analysis of DREB genes in alfalfa (Medicago sativa) in response to cold stress","authors":"Song Sheng, Xinyu Guo, Changzheng Wu, Yucheng Xiang, Shuhui Duan, Weiqin Yang, Wen-Jing Le, Fengchun Cao, Laihua Liu","doi":"10.1080/15592324.2022.2081420","DOIUrl":"https://doi.org/10.1080/15592324.2022.2081420","url":null,"abstract":"ABSTRACT Dehydration-responsive element-binding proteins (DREBs) belong to members of the AP2/ERF transcription factor superfamily, which has been reported to involve various abiotic-stress responses and tolerance in plants. However, research on the DREB-family is still limited in alfalfa (Medicago sativa L.), a forage legume cultivated worldwide. The recent genome-sequence release of the alfalfa cultivar “XinJiangDaYe” allowed us to identify 172 DREBs by a multi-step homolog search. The phylogenetic analysis indicated that such MsDREBs could be classified into 5 groups, namely A-1 (56 members), A-2 (39), A-3 (3), A-4 (61) and 13 (A-5 (13), thus adding substantial new members to the DREB-family in alfalfa. Furthermore, a comprehensive survey in silico of conserved motif, gene structure, molecular weight, and isoelectric point (pI) as well as gene expression was conducted. The resulting data showed that, for cold-stress response, 33 differentially expressed MsDREBs were identified with a threshold of Log2-fold > 1, and most of which were transcriptionally upregulated within 48 h during a cold treatment(s). Moreover, the expression profiling of MsDREBs from two ecotypes of alfalfa subspecies i.e. M. sativa ssp. falcata (F56, from a colder region of Central Asia) and M. sativa ssp. sativa (B47, from Near East) revealed that most of the cold-stress responsive MsDREBs exhibited a significantly lower expression in F56, leading to a proposal of the existence of a distinct mechanism(s) for cold tolerance regulated by DREB-related action, which would have been evolved in alfalfa with a genotypic specificity. Additionally, by examining the transcriptome of a freezing-tolerance species (M. sativa cv. Zhaodong), eight DREBs were found to be implicated in a long-term freezing-stress adaptation with a great potential. Taken together, the current genome-wide identification in alfalfa points to the importance of some MsDREBs in the cold-stress response, providing some promising molecular targets to be functionally characterized for the improvement of cold tolerance in crops including alfalfa.","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":"43 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75467517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nitrogen application alleviates salt stress by enhancing osmotic balance, ROS scavenging, and photosynthesis of rapeseed seedlings (Brassica napus)","authors":"T. Tian, Jingang Wang, Haijiang Wang, Jing Cui, Xiaoyan Shi, Jianghui Song, Weidi Li, M. Zhong, Yue Qiu, Ting Xu","doi":"10.1080/15592324.2022.2081419","DOIUrl":"https://doi.org/10.1080/15592324.2022.2081419","url":null,"abstract":"ABSTRACT Nitrogen application could alleviate salt stress on crops, but the specific physiological mechanism is still unclear. Therefore, in this study, a pot experiment was conducted to explore the effects of different application rates of nitrogen (0, 0.15, 0.30, and 0.45 g·kg−1) on the growth parameters, osmotic adjustment, reactive oxygen species scavenging, and photosynthesis of rapeseed seedlings planted in the soils with different concentrations of sodium chloride (1.5, 3.5, 5.5, and 7.5 g·kg−1). The results showed that nitrogen could alleviate the inhibition of salt on rapeseed growth, and improve the antioxidant enzyme activities and the contents of non-enzymatic substances, K+, soluble protein (SP), soluble sugar (SS), and proline. Besides, there was a significant correlation between the indexes of active oxygen scavenging system, osmoregulation system, and photosynthesis. Therefore, applying appropriate amount of nitrogen can promote the growth and development of rapeseed seedlings under salt stress, accelerate the scavenging of reactive oxygen species, maintain osmotic balance, and promote photosynthesis. This study will improve our understanding on the mechanism by which nitrogen application alleviates salt stress to crops.","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":"28 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2022-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80647439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"N6-methyladenosine–modified RNA acts as a molecular glue that drives liquid–liquid phase separation in plants","authors":"H. Lee, Jiwoo Kim, P. Seo","doi":"10.1080/15592324.2022.2079308","DOIUrl":"https://doi.org/10.1080/15592324.2022.2079308","url":null,"abstract":"ABSTRACT Liquid-like condensates are organized by multivalent intrinsically disordered proteins and RNA molecules. We here demonstrate that N6-methyladenosine (m6A)-modified RNA is widespread in establishing diverse plant cell condensates. Several m6A-reader proteins contain putative prion-like domains, and the ect2/3/4 mutant exhibited reduced formation of key nuclear and cytoplasmic condensates in Arabidopsis.","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":"116 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2022-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88979383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Can electrophysiological information reflect the response of mangrove species to salt stress? A case study of rewatering and Sodium nitroprusside application","authors":"Kashif Ali Solangi, Yanyou Wu, Deke Xing, Waqar Ahmed Qureshi, Mazhar Hussain Tunio, Sher Ali Sheikh, Abdul Shabbir","doi":"10.1080/15592324.2022.2073420","DOIUrl":"https://doi.org/10.1080/15592324.2022.2073420","url":null,"abstract":"ABSTRACT The changes in plant life behaviors and water status are accompanied by electrophysiological activities. In this study, the theoretical relationship between clamping force (CF) and leaf resistance (R), capacitive reactance (XC), inductive reactance (XL), impedance (Z), and capacitance (C) were exposed as 3-parameter exponential decay and linear models based on bioenergetics, respectively, for mangrove species. The intracellular water metabolism parameters and salt transport characteristics were also determined based on mechanical equations with influences of Sodium nitroprusside (SNP) and rewatering (RW). The results show that the inherent capacitance and effective thickness could better represent Aegiceras corniculatum (A. corniculatum) species, and inherent resistance and impedance show obvious effects on Kandelia obovate (K. obovate) species at different salt levels. SNP application shows positive effect on different salt-resistance capacities of A. corniculatum, while K. obovate perform better in RW phase at high salt level. These outcomes indicates that K. obovate is more salt-resistant because RW process is consistent with actual situation, and response of A. corniculatum at high salt stress is irreversible, even in RW. It is concluded that the electrophysiological parameters could be used for the determination of salt-resistant capacities, which gave more enhanced and reliable information of mangroves’ life activities.","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":"39 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2022-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73838802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The cultivable endophytic fungal community of Scutellaria baicalensis: diversity and relevance to flavonoid production by the host","authors":"Xiaoping Cui, Lei Wang, Huiyong Fang, Yuguang Zheng, C. Su","doi":"10.1080/15592324.2022.2068834","DOIUrl":"https://doi.org/10.1080/15592324.2022.2068834","url":null,"abstract":"ABSTRACT Scutellaria baicalensis (SB), a traditional Chinese medicinal plant, is widely used because of its important pharmacological activities. However, the endophytic fungi that promote flavonoid accumulation in SB remain unclear. Therefore, we analyzed the endophytic fungal community of SB and screened the endophytic fungi that might promote flavonoid synthesis in SB. ITS1/ITS4Blast was used to identify the endophytic fungi in SB. In total, 687 strains were identified in 57 genera. The dominant genus in the leaves and stems was Alternaria and that in the roots was Fusarium. Alternaria was the dominant genus in SB collected from all sites and in wild and cultivated SB. Alpha diversity indexes indicated more abundant endophytic fungi in samples from Chengde, the genuine producing area of SB, than in those from other sites. Beta diversity index analysis indicated that SB plants with closer geographical relationships showed more similar endophytic fungal community profiles. Spearman correlation analysis revealed that baicalin, wogonoside, wogonin, and oroxylin A contents were significantly correlated with the relative abundance of Alternaria. Overall, the results indicate the importance of geographical factors in influencing the endophytic fungal community of SB and suggest that the presence of Alternaria spp. might contribute to flavonoid synthesis in SB.","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":"45 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2022-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84351384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A conserved oxalyl-coenzyme A decarboxylase in oxalate catabolism","authors":"N. Cheng, Vincent Paris, Xiaolan Rao, Xiaoqiang Wang, P. Nakata","doi":"10.1080/15592324.2022.2062555","DOIUrl":"https://doi.org/10.1080/15592324.2022.2062555","url":null,"abstract":"ABSTRACT The ability to biosynthesize oxalic acid can provide beneficial functions to plants; however, uncontrolled or prolonged exposure to this strong organic acid results in multiple physiological problems. Such problems include a disruption of membrane integrity, mitochondrial function, metal chelation, and free radical formation. Recent work suggests that a CoA-dependent pathway of oxalate catabolism plays a critical role in regulating tissue oxalate concentrations in plants. Although this CoA-dependent pathway of oxalate catabolism is important, large gaps in our knowledge of the enzymes catalyzing each step remain. Evidence that an oxalyl-CoA decarboxylase (OXC) catalyzes the second step in this pathway, accelerating the conversion of oxalyl-CoA to formyl-CoA, has been reported. Induction studies revealed that OXC gene expression was upregulated in response to an exogenous oxalate supply. Phylogenetic analysis indicates that OXCs are conserved across plant species. Evolutionarily the plant OXCs can be separated into dicot and monocot classes. Multiple sequence alignments and molecular modeling suggest that OXCs have similar functionality with three conserved domains, the N-terminal PYR domain, the middle R domain, and the C-terminal PP domain. Further study of this CoA-dependent pathway of oxalate degradation would benefit efforts to develop new strategies to improve the nutrition quality of crops.","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":"15 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2022-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78477692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ABA activated SnRK2 kinases: an emerging role in plant growth and physiology","authors":"M. Hasan, Xu-Dong Liu, Muhammed Waseem, Guangqian Yao, N. Alabdallah, M. S. Jahan, Xiang-Wen Fang","doi":"10.1080/15592324.2022.2071024","DOIUrl":"https://doi.org/10.1080/15592324.2022.2071024","url":null,"abstract":"ABSTRACT Members of the SNF1-related protein kinase 2 (SnRK2) family are plant-specific serine or threonine kinases that play a pivotal role in the response of plants to abiotic stresses. Members of this plant-specific kinase family have included a critical regulator (SnRK2) of abscisic acid (ABA) response in plants. Plant organ development is governed substantially by the interaction of the SnRK2 and the phytohormone abscisic acid (ABA). Recent research has revealed a synergistic link between SnRK2 and ABA signaling in a plant’s response to stress such as drought and shoot growth. SnRK2 kinases play a dual role in the control of SnRK1 and the development of a plant. The dual role of SnRK2 kinases promotes plant growth under optimal conditions and in the absence of ABA while inhibiting the growth of plants in response to ABA. In this review, we have uncovered the roles of ABA-activated SnRK2 kinases in plants, as well as their physiological mechanisms.","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":"79 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2022-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83368330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}