Plant Signaling & Behavior最新文献_第7页

Targeted for destruction: degradation of singlet oxygen-damaged chloroplasts. 目标破坏:降解单线态氧损伤叶绿体。

IF 2.9 4区生物学

Plant Signaling & Behavior Pub Date : 2022-12-31 DOI: 10.1080/15592324.2022.2084955

Matthew D Lemke, Jesse D Woodson

{"title":"Targeted for destruction: degradation of singlet oxygen-damaged chloroplasts.","authors":"Matthew D Lemke, Jesse D Woodson","doi":"10.1080/15592324.2022.2084955","DOIUrl":"https://doi.org/10.1080/15592324.2022.2084955","url":null,"abstract":"Photosynthesis is an essential process that plants must regulate to survive in dynamic environments. Thus, chloroplasts (the sites of photosynthesis in plant and algae cells) use multiple signaling mechanisms to report their health to the cell. Such signals are poorly understood but often involve reactive oxygen species (ROS) produced from the photosynthetic light reactions. One ROS, singlet oxygen (1O2), can signal to initiate chloroplast degradation, but the cellular machinery involved in identifying and degrading damaged chloroplasts (i.e., chloroplast quality control pathways) is unknown. To provide mechanistic insight into these pathways, two recent studies have investigated degrading chloroplasts in the Arabidopsis thaliana1O2 over-producing plastid ferrochelatase two (fc2) mutant. First, a structural analysis of degrading chloroplasts was performed with electron microscopy, which demonstrated that damaged chloroplasts can protrude into the central vacuole compartment with structures reminiscent of fission-type microautophagy. 1O2-stressed chloroplasts swelled before these interactions, which may be a mechanism for their selective degradation. Second, the roles of autophagosomes and canonical autophagy (macroautophagy) were shown to be dispensable for 1O2-initiated chloroplast degradation. Instead, putative fission-type microautophagy genes were induced by chloroplast 1O2. Here, we discuss how these studies implicate this poorly understood cellular degradation pathway in the dismantling of 1O2-damaged chloroplasts.","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":"17 1","pages":"2084955"},"PeriodicalIF":2.9,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9196835/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10476646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Identification of endophytic fungi with ACC deaminase-producing isolated from halophyte Kosteletzkya Virginica. 产ACC脱氨酶内生真菌的鉴定。

IF 2.9 4区生物学

Plant Signaling & Behavior Pub Date : 2022-12-31 DOI: 10.1080/15592324.2022.2152224

Xiaomin Wang, Zengyuan Tian, Yu Xi, Yuqi Guo

{"title":"Identification of endophytic fungi with ACC deaminase-producing isolated from halophyte Kosteletzkya Virginica.","authors":"Xiaomin Wang, Zengyuan Tian, Yu Xi, Yuqi Guo","doi":"10.1080/15592324.2022.2152224","DOIUrl":"https://doi.org/10.1080/15592324.2022.2152224","url":null,"abstract":"Seashore mallow (Kosteletzkya virginica), as a noninvasive perennial halophytic oilseed-producing dicot, is native from the Gulf to the Atlantic coasts of the U.S. The purpose of our research was to investigate 1-aminocyclopropane-1carboxylic acid deaminase (ACCD) producing endophytic fungi from K.virginica. A total of 59 endophytic fungal strains, isolated from roots in K.virginica of seedlings, were grouped into 12 genera including in Penicillium, Aspergillus, Fusarium, Trichoderma, Rhizopycnis sp., Ceriporia Donk, Trametes sp., Schizophyllum commune sp., Alternaria, Cladosporium, Cylindrocarpon, and Scytalidium according to sequences of ITS. The ACD activity of 10 endophytic fungi isolated was detected. T.asperellum had the highest ACC deaminase activity among all 10 isolated genera of fungal strains, followed by T. viride. Dry weight and fresh weight of plant, plant height, root length, SOD activity, and chlorophyll content of wheat and soybean inoculated with T.asperellum or T. viride was increased compared with non-inoculated control plants under non salt or salt stress. Further analysis showed that T.asperellum or T.viride strains induced downregulation of the expression of ethylene synthesis-related genes such as ACC oxidase (ACO) and ACC synthase (ACS), thereby reducing ethylene synthesis and damage to plants under salt stress. These endophytic fungi can be used as alternative bioinoculants to increase crop yield in saline soil.","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":"17 1","pages":"2152224"},"PeriodicalIF":2.9,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9721417/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10481704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Drug Value of Drynariae Rhizoma Root-Derived Extracellular Vesicles for Neurodegenerative Diseases Based on Proteomics and Bioinformatics. 基于蛋白质组学和生物信息学的干龙根茎细胞外泡治疗神经退行性疾病的药物价值。

IF 2.9 4区生物学

Plant Signaling & Behavior Pub Date : 2022-12-31 DOI: 10.1080/15592324.2022.2129290

Yue Cao, Qing Zhao, Fubin Liu, Lei Zheng, Xingdong Lin, Mingyue Pan, Xuejun Tan, Ge Sun, Kewei Zhao

{"title":"Drug Value of Drynariae Rhizoma Root-Derived Extracellular Vesicles for Neurodegenerative Diseases Based on Proteomics and Bioinformatics.","authors":"Yue Cao, Qing Zhao, Fubin Liu, Lei Zheng, Xingdong Lin, Mingyue Pan, Xuejun Tan, Ge Sun, Kewei Zhao","doi":"10.1080/15592324.2022.2129290","DOIUrl":"https://doi.org/10.1080/15592324.2022.2129290","url":null,"abstract":"Extracellular vesicles (EVs) are nano-sized membrane vesicles released by various cell types. Mammalian EVs have been studied in-depth, but the role of plant EVs has rarely been explored. For the first time, EVs from Drynariae Rhizoma roots were isolated and identified using transmission electron microscopy and a flow nano analyzer. Proteomics and bioinformatics were applied to determine the protein composition and complete the functional analysis of the EVs. Seventy-seven proteins were identified from Drynariae Rhizoma root-derived EVs, with enzymes accounting for 47% of the proteins. All of the enzymes were involved in important biological processes in plants. Most of them, including NAD(P)H-quinone oxidoreductase, were enriched in the oxidative phosphorylation pathway in plants and humans, and Alzheimer's disease, Huntington's disease, and Parkinson's disease, which are associated with oxidative stress in humans. These findings suggested that EVs from Drynariae Rhizoma roots could alleviate such neurological diseases and that enzymes, especially NAD(P)H-quinone oxidoreductase, might play an important role in the process.","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":" ","pages":"2129290"},"PeriodicalIF":2.9,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9542947/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33489070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Cloning and expression analysis of DnMSI1 gene in orchid species Dendrobium nobile Lindl. 兰花品种石斛DnMSI1基因的克隆及表达分析。

IF 2.9 4区生物学

Plant Signaling & Behavior Pub Date : 2022-12-31 Epub Date: 2022-01-10 DOI: 10.1080/15592324.2021.2021649

Baolu Cui, Min Huang, Chongdai Guo, Ruihong Li, Yuqi Wang

引用次数: 3

Development of a GFP biosensor reporter for the unfolded protein response-signaling pathway in plants: incorporation of the bZIP60 intron into the GFP gene. 植物未折叠蛋白反应信号通路的GFP生物传感器报告基因的开发:bZIP60内含子与GFP基因的结合。

IF 2.9 4区生物学

Plant Signaling & Behavior Pub Date : 2022-12-31 DOI: 10.1080/15592324.2022.2098645

Rina Carrillo, David A Christopher

{"title":"Development of a GFP biosensor reporter for the unfolded protein response-signaling pathway in plants: incorporation of the bZIP60 intron into the GFP gene.","authors":"Rina Carrillo, David A Christopher","doi":"10.1080/15592324.2022.2098645","DOIUrl":"https://doi.org/10.1080/15592324.2022.2098645","url":null,"abstract":"The ability to measure the activation of the unfolded protein response (UPR) in plants is important when they are exposed to stressful environments. To this end, we developed a unique and versatile biosensor-reporter system to indicate the activation of UPR in living plant cells. The small cytoplasmically spliced intron from the bZIP60 locus was incorporated into the 5' end of the GFP gene, creating the 35S::bZIP60 intron:GFP construct. When this construct is transiently expressed in Arabidopsis protoplasts, the presence of the bZIP60 intron prevents GFP mRNA from being translated under non-UPR conditions. However, when UPR is activated, the IRE1 kinase/ribonuclease splices this intron from the GFP mRNA and its translation proceeds, generating GFP fluorescence. We demonstrated the utility of the system in Arabidopsis leaf protoplasts treated with DTT, which is a chemical inducer of UPR, followed by visualization and quantification using confocal microscopy. The 35S::bZIP60 intron:GFP construct was also expressed in protoplasts from an overexpressor line containing the coding sequence for the UPR-induced, protein folding chaperone, protein disulfide isomerase-9 (PDI9). PDI9 also influences the strength of the UPR signaling pathway. Protoplasts from WT and PDI9 overexpressor plants treated with DTT exhibited significantly higher GFP fluorescence relative to untreated protoplasts, indicating that the bZIP60 intron was spliced from the GFP mRNA in response to activation of UPR. RT-PCR further confirmed the higher induction of PDI9 and bZIP60 (total and spliced) mRNA levels in DTT-treated protoplasts relative to controls. This system can be adapted for monitoring crop stress and for basic studies dissecting the UPR signaling pathway.","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":" ","pages":"2098645"},"PeriodicalIF":2.9,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302528/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40606940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Chemically defined elicitors activate priming in tomato seedlings. 化学定义的激发子激活番茄幼苗的启动。

IF 2.9 4区生物学

Plant Signaling & Behavior Pub Date : 2022-12-31 DOI: 10.1080/15592324.2022.2095143

Kiran R Kharat, Raveendran Pottathil

{"title":"Chemically defined elicitors activate priming in tomato seedlings.","authors":"Kiran R Kharat, Raveendran Pottathil","doi":"10.1080/15592324.2022.2095143","DOIUrl":"https://doi.org/10.1080/15592324.2022.2095143","url":null,"abstract":"Tomato (Solanum lycopersicum L.) is an important crop that possesses about 35,000 genes. The treatment of plants with elicitors or pathogen attacks causes a cascade of defense reactions. We investigated tomato responses to the BamFXTM solution containing Zn and Cu elicitors and report the results of comparative transcriptome analysis of tomato seeds treated with Zn and Cu elicitors. The seeds were treated with optimum concentrations of Bam-FX solutions and subjected to cold methanolic extraction methods to obtain the secondary metabolites produced within them at different time intervals post-Bam-FX treatment. The metabolite mixture was analyzed using gas chromatography-mass spectrometry (GCMS). In transcriptome sequencing, GO and KEGG analyses revealed that the majority of the DEGs in BamFx-treated tomato was associated with primary and secondary metabolism, plant hormone signal transduction, TF regulation, transport, and responses to stimuli.The secondary metabolites found in the BamFX treated tomato seedlings - Esters of Fumaric acid, Succinic acid etc. The transcript levels of most auxin transporter-encoding genes changed significantly in the BamFX-treated seedlings (e.g., Solyc01g007010.3, a RING-type E3 ubiquitin transferase). The gene Solyc07g061720.3 for Gibberellin 2-oxidase and the Phorbol-ester/DAG-type domain-containing protein (Solyc02g068680.1) associated with the intracellular signaling genes were found upregulated in the BamFx-treated seeds. The time-dependent effect of the BamFX (1:500 for 60 min) was found to be regulating Abscisic acid signaling pathway genes (Solyc09g015380.1). This study identified many candidate genes for future functional analyses and laid a theoretical foundation for an improved understanding of the molecular mechanisms involved in the BamFx treatment of tomatoes to improve stress resistance.","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":"17 1","pages":"2095143"},"PeriodicalIF":2.9,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9746373/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10408825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Building bridges: mycelium-mediated plant-plant electrophysiological communication. 搭建桥梁:菌丝介导的植物-植物电生理通讯。

IF 2.9 4区生物学

Plant Signaling & Behavior Pub Date : 2022-12-31 DOI: 10.1080/15592324.2022.2129291

Matthew Adam Thomas, Robin Lewis Cooper

{"title":"Building bridges: mycelium-mediated plant-plant electrophysiological communication.","authors":"Matthew Adam Thomas, Robin Lewis Cooper","doi":"10.1080/15592324.2022.2129291","DOIUrl":"https://doi.org/10.1080/15592324.2022.2129291","url":null,"abstract":"Whether through root secretions or by emitting volatile organic compounds, plant communication has been well-documented. While electrical activity has been documented in plants and mycorrhizal bodies on the individual and ramet, electrical propagation as a means of communication between plants has been hypothesized but understudied. This study aimed to test the hypothesis that plants can communicate with one another electrically via conductively isolated mycelial pathways. We created a bio-electric circuit linking two plants using a mycelial network grown from a blend of mycorrhizal fungi which was directly inoculated onto potato dextrose agar, or onto the host plants placed on the agar. The mycelium that grew was forced to cross, or \"bridge,\" an air gap between the two islands of agar - thus forming the isolated conductive pathway between plants. Using this plant-fungal biocircuit we assessed electrical propagation between Pisum sativum and Cucumis sativus. We found that electrical signals were reliably conducted across the mycelial bridges from one plant to another upon the induction of a wound response. Our findings provide evidence that mechanical input can be communicated between plant species and opens the door to testing how this information can affect plant and fungal physiology.","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":"17 1","pages":"2129291"},"PeriodicalIF":2.9,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9673936/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10426697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

OsTZF1, a CCCH-tandem zinc finger protein gene, driven under own promoter produces no pleiotropic effects and confers salt and drought tolerance in rice. OsTZF1是一个ccch串联锌指蛋白基因，在自身启动子驱动下不产生多效性，使水稻具有耐盐和耐旱性。

IF 2.9 4区生物学

Plant Signaling & Behavior Pub Date : 2022-12-31 DOI: 10.1080/15592324.2022.2142725

Muhammad Ilyas, Safdar Hussain Shah, Yasunari Fujita, Kyonoshin Maruyama, Kazuo Nakashima, Kazuko Yamaguchi-Shinozaki, Asad Jan

{"title":"OsTZF1, a CCCH-tandem zinc finger protein gene, driven under own promoter produces no pleiotropic effects and confers salt and drought tolerance in rice.","authors":"Muhammad Ilyas, Safdar Hussain Shah, Yasunari Fujita, Kyonoshin Maruyama, Kazuo Nakashima, Kazuko Yamaguchi-Shinozaki, Asad Jan","doi":"10.1080/15592324.2022.2142725","DOIUrl":"https://doi.org/10.1080/15592324.2022.2142725","url":null,"abstract":"Different abiotic stresses induce OsTZF1, a tandem CCCH-type zinc finger domain gene, in rice. Here, we report that transgenic rice plants overexpressing OsTZF1 under own promoter (POsTZF1:OsTZF1-OX [for overexpression]) transferred to soil showed normal growth similar to vector control plants. The POsTZF1:OsTZF1-OX produced normal leaves without any lesion mimic phenotype and exhibited normal seed setting. The POsTZF1:OsTZF1-OX plants showed significantly increased tolerance to salt and drought stresses and enhanced post stress recovery. Microarray analysis revealed a total of 846 genes up-regulated and 360 genes down-regulated in POsTZF1:OsTZF1-OX salt-treated plants. Microarray analysis of POsTZF1:OsTZF1-OX plants showed the regulation of many abiotic stress tolerance genes. These results suggest that OsTZF1-OX under own promoter show abiotic stress tolerance and produces no pleiotropic effect on phenotype of transgenic rice plant.","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":"17 1","pages":"2142725"},"PeriodicalIF":2.9,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9677997/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10426699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Novel roles for Arabidopsis dynamin-related proteins DRP1A and DRP2B in resistance against Botrytis cinerea fungal infection. 拟南芥动力蛋白相关蛋白DRP1A和DRP2B在抗灰葡萄孢真菌感染中的新作用

IF 2.9 4区生物学

Plant Signaling & Behavior Pub Date : 2022-12-31 DOI: 10.1080/15592324.2022.2129296

Grant Mc Gowan, Gayani Ekanayake, Robert A Ingle, Antje Heese

引用次数: 0

Phenotypic effect of growth media on Arabidopsis thaliana root hair growth. 生长培养基对拟南芥根毛生长的表型影响。

IF 2.9 4区生物学

Plant Signaling & Behavior Pub Date : 2022-12-31 DOI: 10.1080/15592324.2022.2104002

Naomi Claeijs, Kris Vissenberg

引用次数: 1